Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054373.t1 | RHN76391.1 | 99.2 | 118 | 1 | 0 | 25 | 142 | 58 | 175 | 2.70E-56 | 228 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054373.t1 | P17092 | 65.9 | 135 | 42 | 2 | 4 | 135 | 1 | 134 | 1.3e-38 | 160.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054373.t1 | B7FMT1 | 99.2 | 118 | 1 | 0 | 25 | 142 | 34 | 151 | 2.0e-56 | 228.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054373 | MS.gene054374 | 0.93701 | 6.93E-98 | -1.69E-46 |
| MS.gene054373 | MS.gene36319 | 0.916989 | 9.16E-86 | -1.69E-46 |
| MS.gene054373 | MS.gene57647 | 0.801175 | 1.02E-48 | -1.69E-46 |
| MS.gene054373 | MS.gene57661 | 0.809554 | 1.79E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGTTTTATTCTTACTAAT+AGG | + | 15294:9695-9714 | MS.gene054373:intergenic | 15.0% |
| ! | AATCAAAGCTATGAAAACAA+TGG | - | 15294:9499-9518 | MS.gene054373:CDS | 25.0% |
| ! | CTAATACGTTTCTTGTATTT+GGG | + | 15294:9593-9612 | MS.gene054373:intergenic | 25.0% |
| AAAGCTATGAAAACAATGGA+AGG | - | 15294:9503-9522 | MS.gene054373:CDS | 30.0% | |
| CCAAATACAAGAAACGTATT+AGG | - | 15294:9591-9610 | MS.gene054373:CDS | 30.0% | |
| CCTAATACGTTTCTTGTATT+TGG | + | 15294:9594-9613 | MS.gene054373:intergenic | 30.0% | |
| !!! | GTTTTCATAGCTTTGATTGA+TGG | + | 15294:9497-9516 | MS.gene054373:intergenic | 30.0% |
| TTTGATTGATGGAAGGGATT+GGG | + | 15294:9486-9505 | MS.gene054373:intergenic | 35.0% | |
| ! | CCTGAAAACGTTTTCAAAGT+TGG | - | 15294:9641-9660 | MS.gene054373:CDS | 35.0% |
| !! | CATAGCTTTGATTGATGGAA+GGG | + | 15294:9492-9511 | MS.gene054373:intergenic | 35.0% |
| !! | CCAACTTTGAAAACGTTTTC+AGG | + | 15294:9644-9663 | MS.gene054373:intergenic | 35.0% |
| !! | TAGGATGAAGAAGAAGTATC+AGG | - | 15294:9610-9629 | MS.gene054373:CDS | 35.0% |
| !! | TCATAGCTTTGATTGATGGA+AGG | + | 15294:9493-9512 | MS.gene054373:intergenic | 35.0% |
| CTCTCAACACCATTTCTCAA+CGG | - | 15294:9400-9419 | MS.gene054373:CDS | 40.0% | |
| CTTTGATTGATGGAAGGGAT+TGG | + | 15294:9487-9506 | MS.gene054373:intergenic | 40.0% | |
| GGTAAGGCTGATAATTCTTC+TGG | - | 15294:9746-9765 | MS.gene054373:CDS | 40.0% | |
| TTGATTGATGGAAGGGATTG+GGG | + | 15294:9485-9504 | MS.gene054373:intergenic | 40.0% | |
| ! | CGTTTCTTGTATTTGGGATG+AGG | + | 15294:9587-9606 | MS.gene054373:intergenic | 40.0% |
| ACGAGTAACTTCCACAGACA+CGG | + | 15294:9558-9577 | MS.gene054373:intergenic | 45.0% | |
| CCATTGTTGCCGTTGAGAAA+TGG | + | 15294:9412-9431 | MS.gene054373:intergenic | 45.0% | |
| CCATTTCTCAACGGCAACAA+TGG | - | 15294:9409-9428 | MS.gene054373:CDS | 45.0% | |
| TCCTGCTAGGAATTCTGGTA+AGG | - | 15294:9730-9749 | MS.gene054373:CDS | 45.0% | |
| TCTTCTTGCTCCGATTGAAG+CGG | + | 15294:9785-9804 | MS.gene054373:intergenic | 45.0% | |
| AGAAGAACAGCCTCAGACTC+AGG | - | 15294:9799-9818 | MS.gene054373:CDS | 50.0% | |
| CAACGACAAAACCGTGTCTG+TGG | - | 15294:9544-9563 | MS.gene054373:CDS | 50.0% | |
| CCAGAATTCCTAGCAGGAAC+AGG | + | 15294:9728-9747 | MS.gene054373:intergenic | 50.0% | |
| CCTCGACATTCCGCTTCAAT+CGG | - | 15294:9772-9791 | MS.gene054373:CDS | 50.0% | |
| CCTGTTCCTGCTAGGAATTC+TGG | - | 15294:9725-9744 | MS.gene054373:CDS | 50.0% | |
| GCCTTACCAGAATTCCTAGC+AGG | + | 15294:9734-9753 | MS.gene054373:intergenic | 50.0% | |
| ! | GGGATAGAGAGTGTGGATGT+GGG | + | 15294:9460-9479 | MS.gene054373:intergenic | 50.0% |
| ! | TGGGATAGAGAGTGTGGATG+TGG | + | 15294:9461-9480 | MS.gene054373:intergenic | 50.0% |
| ! | TTGCTGTTCCTGTTCCTGCT+AGG | - | 15294:9717-9736 | MS.gene054373:CDS | 50.0% |
| !! | AGACACGGTTTTGTCGTTGG+TGG | + | 15294:9543-9562 | MS.gene054373:intergenic | 50.0% |
| !! | CACAGACACGGTTTTGTCGT+TGG | + | 15294:9546-9565 | MS.gene054373:intergenic | 50.0% |
| CCGATTGAAGCGGAATGTCG+AGG | + | 15294:9775-9794 | MS.gene054373:intergenic | 55.0% | |
| TGATGGAAGGGATTGGGGCT+GGG | + | 15294:9480-9499 | MS.gene054373:intergenic | 55.0% | |
| TTGATGGAAGGGATTGGGGC+TGG | + | 15294:9481-9500 | MS.gene054373:intergenic | 55.0% | |
| !! | GGGCTTTGAGAGACGAGTGA+GGG | + | 15294:9440-9459 | MS.gene054373:intergenic | 55.0% |
| !! | TGGGCTTTGAGAGACGAGTG+AGG | + | 15294:9441-9460 | MS.gene054373:intergenic | 55.0% |
| TGGGGCTGGGATAGAGAGTG+TGG | + | 15294:9467-9486 | MS.gene054373:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15294 | gene | 9396 | 9826 | 9396 | ID=MS.gene054373 |
| 15294 | mRNA | 9396 | 9826 | 9396 | ID=MS.gene054373.t1;Parent=MS.gene054373 |
| 15294 | exon | 9396 | 9826 | 9396 | ID=MS.gene054373.t1.exon1;Parent=MS.gene054373.t1 |
| 15294 | CDS | 9396 | 9826 | 9396 | ID=cds.MS.gene054373.t1;Parent=MS.gene054373.t1 |
| Gene Sequence |
| Protein sequence |
