AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054374


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATACGTTTCTTGTATT+TGG 0.021710 2.1:+17968453 None:intergenic
CCTGTTCCTGCTAGGAATTC+TGG 0.226525 2.1:-17968319 MS.gene054374:CDS
CTAATACGTTTCTTGTATTT+GGG 0.263359 2.1:+17968454 None:intergenic
GGTAAGGCTGATAATTCTTC+TGG 0.342682 2.1:-17968298 MS.gene054374:CDS
CCAAATACAAGAAACGTATT+AGG 0.357110 2.1:-17968453 MS.gene054374:CDS
TTTGATTGATGGAAGGGATT+GGG 0.366091 2.1:+17968561 None:intergenic
TTGATGGAAGGGATTGGGGC+TGG 0.379607 2.1:+17968566 None:intergenic
TGATGGAAGGGATTGGGGCT+GGG 0.417770 2.1:+17968567 None:intergenic
TTGCTGTTCCTGTTCCTGCT+AGG 0.444869 2.1:-17968327 MS.gene054374:CDS
CCATTGTTGCCGTTGAGAAA+TGG 0.447061 2.1:+17968637 None:intergenic
CTTTGATTGATGGAAGGGAT+TGG 0.447339 2.1:+17968560 None:intergenic
CCAGAATTCCTAGCAGGAAC+AGG 0.463154 2.1:+17968319 None:intergenic
CGTTTCTTGTATTTGGGATG+AGG 0.463720 2.1:+17968460 None:intergenic
TAGGATGAAGAAGAAGTATC+AGG 0.470403 2.1:-17968434 MS.gene054374:CDS
CATAGCTTTGATTGATGGAA+GGG 0.486622 2.1:+17968555 None:intergenic
AGAAGAACAGCCTCAGACTC+AGG 0.489039 2.1:-17968245 MS.gene054374:CDS
TTGATTGATGGAAGGGATTG+GGG 0.489144 2.1:+17968562 None:intergenic
GGATAGAGAGAGTGTGGATG+TGG 0.489261 2.1:+17968588 None:intergenic
CCTCGACATTCCGCTTCAAT+CGG 0.508100 2.1:-17968272 MS.gene054374:CDS
TCCTGCTAGGAATTCTGGTA+AGG 0.524466 2.1:-17968314 MS.gene054374:CDS
TCTTCTTGCTCCGATTGAAG+CGG 0.531289 2.1:+17968262 None:intergenic
TCATAGCTTTGATTGATGGA+AGG 0.545842 2.1:+17968554 None:intergenic
GGGCTGGGATAGAGAGAGTG+TGG 0.548257 2.1:+17968582 None:intergenic
GCCTTACCAGAATTCCTAGC+AGG 0.562008 2.1:+17968313 None:intergenic
CCATTTCTCAACGGCAACAA+TGG 0.562511 2.1:-17968637 MS.gene054374:CDS
CAAAACTAAGCCTGAGTCTG+AGG 0.567568 2.1:+17968235 None:intergenic
AATCAAAGCTATGAAAACAA+TGG 0.574259 2.1:-17968545 MS.gene054374:CDS
CAACGACAAAACCGTGTCTG+TGG 0.582090 2.1:-17968500 MS.gene054374:CDS
AAAGCTATGAAAACAATGGA+AGG 0.583695 2.1:-17968541 MS.gene054374:CDS
ACGAGTAACTTCCACAGACA+CGG 0.613173 2.1:+17968489 None:intergenic
CTCTCAACACCATTTCTCAA+CGG 0.613389 2.1:-17968646 MS.gene054374:CDS
GGGCTTTGAGAGACGAGTGA+GGG 0.632183 2.1:+17968609 None:intergenic
TGGGCTTTGAGAGACGAGTG+AGG 0.637946 2.1:+17968608 None:intergenic
CCGATTGAAGCGGAATGTCG+AGG 0.647031 2.1:+17968272 None:intergenic
GATAGAGAGAGTGTGGATGT+GGG 0.697666 2.1:+17968589 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGTTTTATTCTTACTAAT+AGG + chr2.1:17968541-17968560 None:intergenic 15.0%
! AATCAAAGCTATGAAAACAA+TGG - chr2.1:17968345-17968364 MS.gene054374:CDS 25.0%
! CTAATACGTTTCTTGTATTT+GGG + chr2.1:17968439-17968458 None:intergenic 25.0%
AAAGCTATGAAAACAATGGA+AGG - chr2.1:17968349-17968368 MS.gene054374:CDS 30.0%
CCAAATACAAGAAACGTATT+AGG - chr2.1:17968437-17968456 MS.gene054374:CDS 30.0%
CCTAATACGTTTCTTGTATT+TGG + chr2.1:17968440-17968459 None:intergenic 30.0%
!!! GTTTTCATAGCTTTGATTGA+TGG + chr2.1:17968343-17968362 None:intergenic 30.0%
TTTGATTGATGGAAGGGATT+GGG + chr2.1:17968332-17968351 None:intergenic 35.0%
! CCTGAAAACGTTTTCAAAGT+TGG - chr2.1:17968487-17968506 MS.gene054374:CDS 35.0%
!! CATAGCTTTGATTGATGGAA+GGG + chr2.1:17968338-17968357 None:intergenic 35.0%
!! CCAACTTTGAAAACGTTTTC+AGG + chr2.1:17968490-17968509 None:intergenic 35.0%
!! TAGGATGAAGAAGAAGTATC+AGG - chr2.1:17968456-17968475 MS.gene054374:CDS 35.0%
!! TCATAGCTTTGATTGATGGA+AGG + chr2.1:17968339-17968358 None:intergenic 35.0%
CTCTCAACACCATTTCTCAA+CGG - chr2.1:17968244-17968263 MS.gene054374:CDS 40.0%
CTTTGATTGATGGAAGGGAT+TGG + chr2.1:17968333-17968352 None:intergenic 40.0%
GGTAAGGCTGATAATTCTTC+TGG - chr2.1:17968592-17968611 MS.gene054374:CDS 40.0%
TTGATTGATGGAAGGGATTG+GGG + chr2.1:17968331-17968350 None:intergenic 40.0%
! CGTTTCTTGTATTTGGGATG+AGG + chr2.1:17968433-17968452 None:intergenic 40.0%
ACGAGTAACTTCCACAGACA+CGG + chr2.1:17968404-17968423 None:intergenic 45.0%
CCATTGTTGCCGTTGAGAAA+TGG + chr2.1:17968256-17968275 None:intergenic 45.0%
CCATTTCTCAACGGCAACAA+TGG - chr2.1:17968253-17968272 MS.gene054374:CDS 45.0%
TCCTGCTAGGAATTCTGGTA+AGG - chr2.1:17968576-17968595 MS.gene054374:CDS 45.0%
TCTTCTTGCTCCGATTGAAG+CGG + chr2.1:17968631-17968650 None:intergenic 45.0%
! GATAGAGAGAGTGTGGATGT+GGG + chr2.1:17968304-17968323 None:intergenic 45.0%
AGAAGAACAGCCTCAGACTC+AGG - chr2.1:17968645-17968664 MS.gene054374:CDS 50.0%
CAACGACAAAACCGTGTCTG+TGG - chr2.1:17968390-17968409 MS.gene054374:CDS 50.0%
CCAGAATTCCTAGCAGGAAC+AGG + chr2.1:17968574-17968593 None:intergenic 50.0%
CCTCGACATTCCGCTTCAAT+CGG - chr2.1:17968618-17968637 MS.gene054374:CDS 50.0%
CCTGTTCCTGCTAGGAATTC+TGG - chr2.1:17968571-17968590 MS.gene054374:CDS 50.0%
GCCTTACCAGAATTCCTAGC+AGG + chr2.1:17968580-17968599 None:intergenic 50.0%
! GGATAGAGAGAGTGTGGATG+TGG + chr2.1:17968305-17968324 None:intergenic 50.0%
! TTGCTGTTCCTGTTCCTGCT+AGG - chr2.1:17968563-17968582 MS.gene054374:CDS 50.0%
!! AGACACGGTTTTGTCGTTGG+TGG + chr2.1:17968389-17968408 None:intergenic 50.0%
!! CACAGACACGGTTTTGTCGT+TGG + chr2.1:17968392-17968411 None:intergenic 50.0%
CCGATTGAAGCGGAATGTCG+AGG + chr2.1:17968621-17968640 None:intergenic 55.0%
TGATGGAAGGGATTGGGGCT+GGG + chr2.1:17968326-17968345 None:intergenic 55.0%
TTGATGGAAGGGATTGGGGC+TGG + chr2.1:17968327-17968346 None:intergenic 55.0%
!! GGGCTTTGAGAGACGAGTGA+GGG + chr2.1:17968284-17968303 None:intergenic 55.0%
!! TGGGCTTTGAGAGACGAGTG+AGG + chr2.1:17968285-17968304 None:intergenic 55.0%
GGGCTGGGATAGAGAGAGTG+TGG + chr2.1:17968311-17968330 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 17968240 17968672 17968240 ID=MS.gene054374
chr2.1 mRNA 17968240 17968672 17968240 ID=MS.gene054374.t1;Parent=MS.gene054374
chr2.1 exon 17968240 17968672 17968240 ID=MS.gene054374.t1.exon1;Parent=MS.gene054374.t1
chr2.1 CDS 17968240 17968672 17968240 ID=cds.MS.gene054374.t1;Parent=MS.gene054374.t1
Gene Sequence

>MS.gene054374

CACGCTCTCAACACCATTTCTCAACGGCAACAATGGCGTCGCCCTCACTCGTCTCTCAAAGCCCACATCCACACTCTCTCTATCCCAGCCCCAATCCCTTCCATCAATCAAAGCTATGAAAACAATGGAAGGAAAAGTTGTGTGTGCCACCAACGACAAAACCGTGTCTGTGGAAGTTACTCGTTTAGCACCTCATCCCAAATACAAGAAACGTATTAGGATGAAGAAGAAGTATCAGGCTCATGATCCTGAAAACGTTTTCAAAGTTGGTGATGTTGTTCAGCTTTGTAAAATTAGACCTATTAGTAAGAATAAAACTTTTCTTGCTGTTCCTGTTCCTGCTAGGAATTCTGGTAAGGCTGATAATTCTTCTGGTGACCTCGACATTCCGCTTCAATCGGAGCAAGAAGAACAGCCTCAGACTCAGGCTTAG

Protein sequence

>MS.gene054374.t1

HALNTISQRQQWRRPHSSLKAHIHTLSIPAPIPSINQSYENNGRKSCVCHQRQNRVCGSYSFSTSSQIQETYUDEEEVSGSUSUKRFQSWUCCSALUNUTYUUEUNFSCCSCSCUEFWUGUUFFWUPRHSASIGARRTASDSGL