Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57661.t1 | XP_024636800.1 | 98.5 | 67 | 1 | 0 | 9 | 75 | 38 | 104 | 9.90E-28 | 132.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57661.t1 | P81748 | 43.1 | 65 | 37 | 0 | 9 | 73 | 37 | 101 | 5.7e-08 | 58.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57661.t1 | G7JUA1 | 98.5 | 67 | 1 | 0 | 9 | 75 | 776 | 842 | 7.2e-28 | 132.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene57661 | 0.80455 | 2.05E-49 | -1.69E-46 |
| MS.gene049222 | MS.gene57661 | 0.805191 | 1.50E-49 | -1.69E-46 |
| MS.gene049232 | MS.gene57661 | 0.80771 | 4.43E-50 | -1.69E-46 |
| MS.gene049285 | MS.gene57661 | 0.844826 | 5.94E-59 | -1.69E-46 |
| MS.gene049320 | MS.gene57661 | 0.849279 | 3.58E-60 | -1.69E-46 |
| MS.gene05010 | MS.gene57661 | 0.82549 | 4.56E-54 | -1.69E-46 |
| MS.gene050163 | MS.gene57661 | 0.826738 | 2.30E-54 | -1.69E-46 |
| MS.gene050165 | MS.gene57661 | 0.837626 | 4.65E-57 | -1.69E-46 |
| MS.gene050333 | MS.gene57661 | 0.829247 | 5.73E-55 | -1.69E-46 |
| MS.gene050504 | MS.gene57661 | 0.847564 | 1.07E-59 | -1.69E-46 |
| MS.gene050518 | MS.gene57661 | 0.854864 | 9.26E-62 | -1.69E-46 |
| MS.gene050612 | MS.gene57661 | 0.844409 | 7.70E-59 | -1.69E-46 |
| MS.gene050699 | MS.gene57661 | 0.802102 | 6.58E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene57661 | 0.808907 | 2.46E-50 | -1.69E-46 |
| MS.gene050986 | MS.gene57661 | 0.809972 | 1.45E-50 | -1.69E-46 |
| MS.gene05100 | MS.gene57661 | 0.810088 | 1.37E-50 | -1.69E-46 |
| MS.gene051551 | MS.gene57661 | 0.804718 | 1.89E-49 | -1.69E-46 |
| MS.gene051810 | MS.gene57661 | 0.833589 | 4.89E-56 | -1.69E-46 |
| MS.gene053213 | MS.gene57661 | 0.816913 | 4.33E-52 | -1.69E-46 |
| MS.gene053286 | MS.gene57661 | 0.823422 | 1.40E-53 | -1.69E-46 |
| MS.gene054373 | MS.gene57661 | 0.809554 | 1.79E-50 | -1.69E-46 |
| MS.gene054374 | MS.gene57661 | 0.857534 | 1.53E-62 | -1.69E-46 |
| MS.gene054642 | MS.gene57661 | 0.859164 | 5.00E-63 | -1.69E-46 |
| MS.gene054954 | MS.gene57661 | 0.850502 | 1.63E-60 | -1.69E-46 |
| MS.gene055362 | MS.gene57661 | 0.832113 | 1.14E-55 | -1.69E-46 |
| MS.gene055502 | MS.gene57661 | 0.832272 | 1.04E-55 | -1.69E-46 |
| MS.gene055577 | MS.gene57661 | 0.804261 | 2.35E-49 | -1.69E-46 |
| MS.gene055634 | MS.gene57661 | 0.823975 | 1.04E-53 | -1.69E-46 |
| MS.gene055638 | MS.gene57661 | 0.812685 | 3.74E-51 | -1.69E-46 |
| MS.gene055751 | MS.gene57661 | 0.802353 | 5.84E-49 | -1.69E-46 |
| MS.gene055776 | MS.gene57661 | 0.818681 | 1.73E-52 | -1.69E-46 |
| MS.gene056374 | MS.gene57661 | 0.802236 | 6.17E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene57661 | 0.817283 | 3.57E-52 | -1.69E-46 |
| MS.gene056510 | MS.gene57661 | 0.811153 | 8.07E-51 | -1.69E-46 |
| MS.gene056657 | MS.gene57661 | 0.847967 | 8.27E-60 | -1.69E-46 |
| MS.gene058419 | MS.gene57661 | 0.841869 | 3.66E-58 | -1.69E-46 |
| MS.gene058773 | MS.gene57661 | 0.829909 | 3.95E-55 | -1.69E-46 |
| MS.gene059022 | MS.gene57661 | 0.844776 | 6.13E-59 | -1.69E-46 |
| MS.gene059029 | MS.gene57661 | 0.810178 | 1.31E-50 | -1.69E-46 |
| MS.gene05913 | MS.gene57661 | 0.805662 | 1.20E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene57661 | 0.835116 | 2.02E-56 | -1.69E-46 |
| MS.gene059718 | MS.gene57661 | 0.801853 | 7.40E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene57661 | 0.829797 | 4.21E-55 | -1.69E-46 |
| MS.gene060337 | MS.gene57661 | -0.820103 | 8.19E-53 | -1.69E-46 |
| MS.gene060703 | MS.gene57661 | 0.870724 | 1.18E-66 | -1.69E-46 |
| MS.gene06077 | MS.gene57661 | 0.820817 | 5.61E-53 | -1.69E-46 |
| MS.gene060982 | MS.gene57661 | 0.805678 | 1.19E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57661.t1 | MTR_4g127370 | 98.507 | 67 | 1 | 0 | 9 | 75 | 776 | 842 | 2.48e-39 | 138 |
| MS.gene57661.t1 | MTR_4g127360 | 79.104 | 67 | 14 | 0 | 9 | 75 | 38 | 104 | 1.82e-32 | 109 |
| MS.gene57661.t1 | MTR_4g014040 | 63.768 | 69 | 25 | 0 | 9 | 77 | 38 | 106 | 2.32e-28 | 99.4 |
| MS.gene57661.t1 | MTR_4g014040 | 64.706 | 68 | 24 | 0 | 8 | 75 | 37 | 104 | 1.42e-27 | 97.1 |
| MS.gene57661.t1 | MTR_4g014040 | 64.706 | 68 | 24 | 0 | 9 | 76 | 38 | 105 | 3.16e-27 | 97.4 |
| MS.gene57661.t1 | MTR_7g059460 | 57.353 | 68 | 29 | 0 | 8 | 75 | 37 | 104 | 9.91e-23 | 84.7 |
| MS.gene57661.t1 | MTR_7g059460 | 54.930 | 71 | 32 | 0 | 8 | 78 | 37 | 107 | 1.12e-22 | 84.7 |
| MS.gene57661.t1 | MTR_7g070250 | 47.143 | 70 | 37 | 0 | 8 | 77 | 37 | 106 | 1.32e-19 | 76.6 |
| MS.gene57661.t1 | MTR_4g013730 | 47.761 | 67 | 35 | 0 | 8 | 74 | 37 | 103 | 1.15e-18 | 74.3 |
| MS.gene57661.t1 | MTR_0562s0020 | 47.761 | 67 | 35 | 0 | 8 | 74 | 37 | 103 | 1.15e-18 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57661.t1 | AT5G57170 | 87.879 | 66 | 8 | 0 | 9 | 74 | 38 | 103 | 1.35e-35 | 117 |
| MS.gene57661.t1 | AT5G57170 | 86.567 | 67 | 9 | 0 | 8 | 74 | 37 | 103 | 3.37e-35 | 116 |
| MS.gene57661.t1 | AT5G01650 | 60.870 | 69 | 27 | 0 | 8 | 76 | 37 | 105 | 1.88e-27 | 96.7 |
| MS.gene57661.t1 | AT5G01650 | 60.000 | 70 | 28 | 0 | 8 | 77 | 37 | 106 | 3.33e-27 | 96.3 |
| MS.gene57661.t1 | AT5G01650 | 60.000 | 70 | 28 | 0 | 8 | 77 | 37 | 106 | 3.33e-27 | 96.3 |
| MS.gene57661.t1 | AT5G01650 | 61.765 | 68 | 26 | 0 | 8 | 75 | 37 | 104 | 6.22e-27 | 95.5 |
| MS.gene57661.t1 | AT3G51660 | 41.429 | 70 | 41 | 0 | 10 | 79 | 39 | 108 | 5.65e-14 | 62.4 |
Find 19 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGATATCAATTGGCGGACT+TGG | 0.336187 | 8.4:+7312131 | MS.gene57661:CDS |
| ATAAAATTTGATGTAGAATC+TGG | 0.367314 | 8.4:-7312225 | None:intergenic |
| CAATGTATAGTTTAGTTTGA+AGG | 0.396267 | 8.4:-7312196 | None:intergenic |
| GGTGGAGTGCCTATTGCATT+TGG | 0.425683 | 8.4:+7312077 | MS.gene57661:CDS |
| ACTGAAGAGCCGGCTGCCTA+TGG | 0.457767 | 8.4:+7312104 | MS.gene57661:CDS |
| TCAGTCCCACCAAATGCAAT+AGG | 0.463249 | 8.4:-7312086 | None:intergenic |
| TATGGAGAGCTGATATCAAT+TGG | 0.477027 | 8.4:+7312122 | MS.gene57661:CDS |
| ATCATCACATACTGAAACAA+AGG | 0.508697 | 8.4:-7312044 | None:intergenic |
| GGAGTGCCTATTGCATTTGG+TGG | 0.518848 | 8.4:+7312080 | MS.gene57661:CDS |
| AATTGATATCAGCTCTCCAT+AGG | 0.553233 | 8.4:-7312120 | None:intergenic |
| TCACACACAGTCTCCGCCAT+AGG | 0.558423 | 8.4:-7312670 | None:intergenic |
| GAGTGCCTATTGCATTTGGT+GGG | 0.560113 | 8.4:+7312081 | MS.gene57661:CDS |
| GGAGAGCTGATATCAATTGG+CGG | 0.574098 | 8.4:+7312125 | MS.gene57661:CDS |
| ATCAGCTCTCCATAGGCAGC+CGG | 0.584190 | 8.4:-7312113 | None:intergenic |
| ATTTGGTGGGACTGAAGAGC+CGG | 0.598688 | 8.4:+7312094 | MS.gene57661:CDS |
| AACACCGTATCAGTAGCCTA+TGG | 0.601391 | 8.4:+7312654 | MS.gene57661:CDS |
| TCCGCCATAGGCTACTGATA+CGG | 0.627746 | 8.4:-7312658 | None:intergenic |
| ACCGTATCAGTAGCCTATGG+CGG | 0.689344 | 8.4:+7312657 | MS.gene57661:CDS |
| TATGGCGGAGACTGTGTGTG+AGG | 0.693756 | 8.4:+7312672 | MS.gene57661:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAACAGTTTCAATATAATA+TGG | - | chr8.4:7312389-7312408 | None:intergenic | 15.0% |
| !! | TATTAAATTATTACTGTTCT+GGG | + | chr8.4:7312463-7312482 | MS.gene57661:intron | 15.0% |
| !! | ATAAAATTTGATGTAGAATC+TGG | - | chr8.4:7312228-7312247 | None:intergenic | 20.0% |
| !! | ATTAAATTATTACTGTTCTG+GGG | + | chr8.4:7312464-7312483 | MS.gene57661:intron | 20.0% |
| !! | GTATTAAATTATTACTGTTC+TGG | + | chr8.4:7312462-7312481 | MS.gene57661:intron | 20.0% |
| ! | AAGAAATCGTAAATTTACCT+CGG | - | chr8.4:7312255-7312274 | None:intergenic | 25.0% |
| ! | GTATTGTTCTTATAAAGTCT+TGG | + | chr8.4:7312625-7312644 | MS.gene57661:intron | 25.0% |
| !! | ATACATGAAATAGAGTACTA+TGG | - | chr8.4:7312609-7312628 | None:intergenic | 25.0% |
| !! | TATGTGATGATTTTGTTGAA+TGG | + | chr8.4:7312056-7312075 | MS.gene57661:CDS | 25.0% |
| !!! | CAATGTATAGTTTAGTTTGA+AGG | - | chr8.4:7312199-7312218 | None:intergenic | 25.0% |
| ACGAGTTAGAATGTATGAAA+TGG | - | chr8.4:7312351-7312370 | None:intergenic | 30.0% | |
| ATATATATGACTGTGAATGC+TGG | + | chr8.4:7312290-7312309 | MS.gene57661:intron | 30.0% | |
| ATCATCACATACTGAAACAA+AGG | - | chr8.4:7312047-7312066 | None:intergenic | 30.0% | |
| GTTTATTACTCTGAACTTAG+AGG | + | chr8.4:7312404-7312423 | MS.gene57661:intron | 30.0% | |
| AATTGATATCAGCTCTCCAT+AGG | - | chr8.4:7312123-7312142 | None:intergenic | 35.0% | |
| AGGTTTCTCATTGTGTTTGT+TGG | + | chr8.4:7312424-7312443 | MS.gene57661:intron | 35.0% | |
| TATGGAGAGCTGATATCAAT+TGG | + | chr8.4:7312122-7312141 | MS.gene57661:CDS | 35.0% | |
| ! | CATCAAATTTTATGACTCCG+AGG | + | chr8.4:7312235-7312254 | MS.gene57661:CDS | 35.0% |
| ! | CTCAGTTTTGCATTAACACT+TGG | - | chr8.4:7312158-7312177 | None:intergenic | 35.0% |
| ! | GTGATGATTTTGTTGAATGG+TGG | + | chr8.4:7312059-7312078 | MS.gene57661:CDS | 35.0% |
| ! | TTTCTCATTGTGTTTGTTGG+AGG | + | chr8.4:7312427-7312446 | MS.gene57661:intron | 35.0% |
| AACACCGTATCAGTAGCCTA+TGG | + | chr8.4:7312654-7312673 | MS.gene57661:CDS | 45.0% | |
| GAGTGCCTATTGCATTTGGT+GGG | + | chr8.4:7312081-7312100 | MS.gene57661:CDS | 45.0% | |
| GGAGAGCTGATATCAATTGG+CGG | + | chr8.4:7312125-7312144 | MS.gene57661:CDS | 45.0% | |
| TCAGTCCCACCAAATGCAAT+AGG | - | chr8.4:7312089-7312108 | None:intergenic | 45.0% | |
| TTGTTGGAGGAGGCAAAGAT+AGG | + | chr8.4:7312440-7312459 | MS.gene57661:intron | 45.0% | |
| ! | CTCATTGTGTTTGTTGGAGG+AGG | + | chr8.4:7312430-7312449 | MS.gene57661:intron | 45.0% |
| !! | CTGATATCAATTGGCGGACT+TGG | + | chr8.4:7312131-7312150 | MS.gene57661:CDS | 45.0% |
| ACCGTATCAGTAGCCTATGG+CGG | + | chr8.4:7312657-7312676 | MS.gene57661:CDS | 50.0% | |
| TCCGCCATAGGCTACTGATA+CGG | - | chr8.4:7312661-7312680 | None:intergenic | 50.0% | |
| ! | ATTTGGTGGGACTGAAGAGC+CGG | + | chr8.4:7312094-7312113 | MS.gene57661:CDS | 50.0% |
| ! | GGAGTGCCTATTGCATTTGG+TGG | + | chr8.4:7312080-7312099 | MS.gene57661:CDS | 50.0% |
| ! | GGTGGAGTGCCTATTGCATT+TGG | + | chr8.4:7312077-7312096 | MS.gene57661:CDS | 50.0% |
| ATCAGCTCTCCATAGGCAGC+CGG | - | chr8.4:7312116-7312135 | None:intergenic | 55.0% | |
| TCACACACAGTCTCCGCCAT+AGG | - | chr8.4:7312673-7312692 | None:intergenic | 55.0% | |
| ACTGAAGAGCCGGCTGCCTA+TGG | + | chr8.4:7312104-7312123 | MS.gene57661:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 7312032 | 7312692 | 7312032 | ID=MS.gene57661 |
| chr8.4 | mRNA | 7312032 | 7312692 | 7312032 | ID=MS.gene57661.t1;Parent=MS.gene57661 |
| chr8.4 | exon | 7312032 | 7312256 | 7312032 | ID=MS.gene57661.t1.exon1;Parent=MS.gene57661.t1 |
| chr8.4 | CDS | 7312032 | 7312256 | 7312032 | ID=cds.MS.gene57661.t1;Parent=MS.gene57661.t1 |
| chr8.4 | exon | 7312642 | 7312692 | 7312642 | ID=MS.gene57661.t1.exon2;Parent=MS.gene57661.t1 |
| chr8.4 | CDS | 7312642 | 7312692 | 7312642 | ID=cds.MS.gene57661.t1;Parent=MS.gene57661.t1 |
| Gene Sequence |
| Protein sequence |
