AlfalfaGEDB | MS.gene36319

M. sativa cultivar XinJiangDaYe / MS.gene36319

KEGG: K02961
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00366.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36319.t1	RHN76391.1	99.3	151	1	0	1	151	25	175	2.80E-75	291.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36319.t1	P17092	71.3	129	33	3	19	144	7	134	1.1e-40	167.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36319.t1	B7FMT1	99.3	151	1	0	1	151	1	151	2.0e-75	291.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene36319	0.805697	1.18E-49	-1.69E-46
MS.gene049285	MS.gene36319	0.834403	3.06E-56	-1.69E-46
MS.gene049320	MS.gene36319	0.831123	1.99E-55	-1.69E-46
MS.gene050163	MS.gene36319	0.850019	2.23E-60	-1.69E-46
MS.gene050165	MS.gene36319	0.844126	9.17E-59	-1.69E-46
MS.gene050333	MS.gene36319	0.826709	2.34E-54	-1.69E-46
MS.gene050504	MS.gene36319	0.832142	1.12E-55	-1.69E-46
MS.gene050518	MS.gene36319	0.855596	5.67E-62	-1.69E-46
MS.gene050612	MS.gene36319	0.838771	2.36E-57	-1.69E-46
MS.gene050950	MS.gene36319	0.803559	3.29E-49	-1.69E-46
MS.gene05100	MS.gene36319	0.816658	4.94E-52	-1.69E-46
MS.gene051810	MS.gene36319	0.819583	1.08E-52	-1.69E-46
MS.gene053213	MS.gene36319	0.825296	5.06E-54	-1.69E-46
MS.gene053286	MS.gene36319	0.827485	1.52E-54	-1.69E-46
MS.gene053322	MS.gene36319	0.807306	5.39E-50	-1.69E-46
MS.gene054373	MS.gene36319	0.916989	9.16E-86	-1.69E-46
MS.gene054374	MS.gene36319	0.988724	3.73E-175	-1.69E-46
MS.gene054408	MS.gene36319	0.821093	4.85E-53	-1.69E-46
MS.gene054642	MS.gene36319	0.8433	1.53E-58	-1.69E-46
MS.gene054954	MS.gene36319	0.812091	5.05E-51	-1.69E-46
MS.gene055362	MS.gene36319	0.804566	2.03E-49	-1.69E-46
MS.gene055499	MS.gene36319	0.804278	2.33E-49	-1.69E-46
MS.gene055502	MS.gene36319	0.817289	3.56E-52	-1.69E-46
MS.gene055748	MS.gene36319	0.811665	6.25E-51	-1.69E-46
MS.gene055751	MS.gene36319	0.807158	5.79E-50	-1.69E-46
MS.gene056657	MS.gene36319	0.828326	9.56E-55	-1.69E-46
MS.gene056692	MS.gene36319	0.810798	9.64E-51	-1.69E-46
MS.gene057011	MS.gene36319	0.812399	4.32E-51	-1.69E-46
MS.gene058130	MS.gene36319	0.806407	8.35E-50	-1.69E-46
MS.gene05837	MS.gene36319	0.813148	2.96E-51	-1.69E-46
MS.gene058419	MS.gene36319	0.82188	3.19E-53	-1.69E-46
MS.gene058544	MS.gene36319	0.80386	2.85E-49	-1.69E-46
MS.gene058773	MS.gene36319	0.815238	1.02E-51	-1.69E-46
MS.gene059022	MS.gene36319	0.81378	2.15E-51	-1.69E-46
MS.gene059029	MS.gene36319	0.829492	4.99E-55	-1.69E-46
MS.gene059677	MS.gene36319	0.824971	6.05E-54	-1.69E-46
MS.gene059718	MS.gene36319	0.8001	1.69E-48	-1.69E-46
MS.gene059960	MS.gene36319	0.834166	3.51E-56	-1.69E-46
MS.gene06077	MS.gene36319	0.820019	8.56E-53	-1.69E-46
MS.gene06092	MS.gene36319	0.810049	1.40E-50	-1.69E-46
MS.gene061150	MS.gene36319	0.820977	5.16E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36319.t1	MTR_2g101770	99.338	151	1	0	1	151	1	151	6.65e-110	308

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36319.t1	AT1G79850	61.765	136	47	3	10	143	15	147	3.35e-52	163

Find 37 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTAATACGTTTCTTGTATT+TGG	0.021710	2.2:-1940491	None:intergenic
CCTGTTCCTGCTAGGAATTC+TGG	0.226525	2.2:+1940625	MS.gene36319:CDS
CTAATACGTTTCTTGTATTT+GGG	0.263359	2.2:-1940490	None:intergenic
GGTAAGGCTGATAATTCTTC+TGG	0.342682	2.2:+1940646	MS.gene36319:CDS
CCAAATACAAGAAACGTATT+AGG	0.357110	2.2:+1940491	MS.gene36319:CDS
TTTGATTGATGGAAGGGATT+GGG	0.366091	2.2:-1940383	None:intergenic
TTGATGGAAGGGATTGGGGC+TGG	0.379607	2.2:-1940378	None:intergenic
TGATGGAAGGGATTGGGGCT+GGG	0.417770	2.2:-1940377	None:intergenic
TTGCTGTTCCTGTTCCTGCT+AGG	0.444869	2.2:+1940617	MS.gene36319:CDS
CCATTGTTGCCGTTGAGAAA+TGG	0.447061	2.2:-1940307	None:intergenic
CTTTGATTGATGGAAGGGAT+TGG	0.447339	2.2:-1940384	None:intergenic
CCAGAATTCCTAGCAGGAAC+AGG	0.463154	2.2:-1940625	None:intergenic
CGTTTCTTGTATTTGGGATG+AGG	0.463720	2.2:-1940484	None:intergenic
TAGGATGAAGAAGAAGTATC+AGG	0.470403	2.2:+1940510	MS.gene36319:CDS
CATAGCTTTGATTGATGGAA+GGG	0.486622	2.2:-1940389	None:intergenic
AGAAGAACAGCCTCAGACTC+AGG	0.489039	2.2:+1940699	MS.gene36319:CDS
TTGATTGATGGAAGGGATTG+GGG	0.489144	2.2:-1940382	None:intergenic
GGATAGAGAGAGTGTGGATG+TGG	0.489261	2.2:-1940356	None:intergenic
CCTCGACATTCCGCTTCAAT+CGG	0.508100	2.2:+1940672	MS.gene36319:CDS
TCCTGCTAGGAATTCTGGTA+AGG	0.524466	2.2:+1940630	MS.gene36319:CDS
TCTTCTTGCTCCGATTGAAG+CGG	0.531289	2.2:-1940682	None:intergenic
TCATAGCTTTGATTGATGGA+AGG	0.545842	2.2:-1940390	None:intergenic
GGGCTGGGATAGAGAGAGTG+TGG	0.548257	2.2:-1940362	None:intergenic
GCCTTACCAGAATTCCTAGC+AGG	0.562008	2.2:-1940631	None:intergenic
CCATTTCTCAACGGCAACAA+TGG	0.562511	2.2:+1940307	MS.gene36319:CDS
AAATGGTGTTGAGAGCGTGC+TGG	0.564865	2.2:-1940290	None:intergenic
CAAAACTAAGCCTGAGTCTG+AGG	0.567568	2.2:-1940709	None:intergenic
AATCAAAGCTATGAAAACAA+TGG	0.574259	2.2:+1940399	MS.gene36319:CDS
AATGGTGTTGAGAGCGTGCT+GGG	0.576801	2.2:-1940289	None:intergenic
CAACGACAAAACCGTGTCTG+TGG	0.582090	2.2:+1940444	MS.gene36319:CDS
AAAGCTATGAAAACAATGGA+AGG	0.583695	2.2:+1940403	MS.gene36319:CDS
ACGAGTAACTTCCACAGACA+CGG	0.613173	2.2:-1940455	None:intergenic
CTCTCAACACCATTTCTCAA+CGG	0.613389	2.2:+1940298	MS.gene36319:CDS
GGGCTTTGAGAGACGAGTGA+GGG	0.632183	2.2:-1940335	None:intergenic
TGGGCTTTGAGAGACGAGTG+AGG	0.637946	2.2:-1940336	None:intergenic
CCGATTGAAGCGGAATGTCG+AGG	0.647031	2.2:-1940672	None:intergenic
GATAGAGAGAGTGTGGATGT+GGG	0.697666	2.2:-1940355	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAGTTTTATTCTTACTAAT+AGG	-	chr2.2:1940595-1940614	None:intergenic	15.0%
!	AATCAAAGCTATGAAAACAA+TGG	+	chr2.2:1940399-1940418	MS.gene36319:CDS	25.0%
!	CTAATACGTTTCTTGTATTT+GGG	-	chr2.2:1940493-1940512	None:intergenic	25.0%
	AAAGCTATGAAAACAATGGA+AGG	+	chr2.2:1940403-1940422	MS.gene36319:CDS	30.0%
	CCAAATACAAGAAACGTATT+AGG	+	chr2.2:1940491-1940510	MS.gene36319:CDS	30.0%
	CCTAATACGTTTCTTGTATT+TGG	-	chr2.2:1940494-1940513	None:intergenic	30.0%
!!!	GTTTTCATAGCTTTGATTGA+TGG	-	chr2.2:1940397-1940416	None:intergenic	30.0%
	TTTGATTGATGGAAGGGATT+GGG	-	chr2.2:1940386-1940405	None:intergenic	35.0%
!	CCTGAAAACGTTTTCAAAGT+TGG	+	chr2.2:1940541-1940560	MS.gene36319:CDS	35.0%
!!	CATAGCTTTGATTGATGGAA+GGG	-	chr2.2:1940392-1940411	None:intergenic	35.0%
!!	CCAACTTTGAAAACGTTTTC+AGG	-	chr2.2:1940544-1940563	None:intergenic	35.0%
!!	TAGGATGAAGAAGAAGTATC+AGG	+	chr2.2:1940510-1940529	MS.gene36319:CDS	35.0%
!!	TCATAGCTTTGATTGATGGA+AGG	-	chr2.2:1940393-1940412	None:intergenic	35.0%
	CTCTCAACACCATTTCTCAA+CGG	+	chr2.2:1940298-1940317	MS.gene36319:CDS	40.0%
	CTTTGATTGATGGAAGGGAT+TGG	-	chr2.2:1940387-1940406	None:intergenic	40.0%
	GGTAAGGCTGATAATTCTTC+TGG	+	chr2.2:1940646-1940665	MS.gene36319:CDS	40.0%
	TTGATTGATGGAAGGGATTG+GGG	-	chr2.2:1940385-1940404	None:intergenic	40.0%
!	CGTTTCTTGTATTTGGGATG+AGG	-	chr2.2:1940487-1940506	None:intergenic	40.0%
	ACGAGTAACTTCCACAGACA+CGG	-	chr2.2:1940458-1940477	None:intergenic	45.0%
	CCATTGTTGCCGTTGAGAAA+TGG	-	chr2.2:1940310-1940329	None:intergenic	45.0%
	CCATTTCTCAACGGCAACAA+TGG	+	chr2.2:1940307-1940326	MS.gene36319:CDS	45.0%
	TCCTGCTAGGAATTCTGGTA+AGG	+	chr2.2:1940630-1940649	MS.gene36319:CDS	45.0%
	TCTTCTTGCTCCGATTGAAG+CGG	-	chr2.2:1940685-1940704	None:intergenic	45.0%
!	GATAGAGAGAGTGTGGATGT+GGG	-	chr2.2:1940358-1940377	None:intergenic	45.0%
	AGAAGAACAGCCTCAGACTC+AGG	+	chr2.2:1940699-1940718	MS.gene36319:CDS	50.0%
	CAACGACAAAACCGTGTCTG+TGG	+	chr2.2:1940444-1940463	MS.gene36319:CDS	50.0%
	CCAGAATTCCTAGCAGGAAC+AGG	-	chr2.2:1940628-1940647	None:intergenic	50.0%
	CCTCGACATTCCGCTTCAAT+CGG	+	chr2.2:1940672-1940691	MS.gene36319:CDS	50.0%
	CCTGTTCCTGCTAGGAATTC+TGG	+	chr2.2:1940625-1940644	MS.gene36319:CDS	50.0%
	GCCTTACCAGAATTCCTAGC+AGG	-	chr2.2:1940634-1940653	None:intergenic	50.0%
!	AAATGGTGTTGAGAGCGTGC+TGG	-	chr2.2:1940293-1940312	None:intergenic	50.0%
!	AATGGTGTTGAGAGCGTGCT+GGG	-	chr2.2:1940292-1940311	None:intergenic	50.0%
!	GGATAGAGAGAGTGTGGATG+TGG	-	chr2.2:1940359-1940378	None:intergenic	50.0%
!	TTGCTGTTCCTGTTCCTGCT+AGG	+	chr2.2:1940617-1940636	MS.gene36319:CDS	50.0%
!!	AGACACGGTTTTGTCGTTGG+TGG	-	chr2.2:1940443-1940462	None:intergenic	50.0%
!!	CACAGACACGGTTTTGTCGT+TGG	-	chr2.2:1940446-1940465	None:intergenic	50.0%
	CCGATTGAAGCGGAATGTCG+AGG	-	chr2.2:1940675-1940694	None:intergenic	55.0%
	TGATGGAAGGGATTGGGGCT+GGG	-	chr2.2:1940380-1940399	None:intergenic	55.0%
	TTGATGGAAGGGATTGGGGC+TGG	-	chr2.2:1940381-1940400	None:intergenic	55.0%
!!	GGGCTTTGAGAGACGAGTGA+GGG	-	chr2.2:1940338-1940357	None:intergenic	55.0%
!!	TGGGCTTTGAGAGACGAGTG+AGG	-	chr2.2:1940339-1940358	None:intergenic	55.0%
	GGGCTGGGATAGAGAGAGTG+TGG	-	chr2.2:1940365-1940384	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	1940271	1940726	1940271	ID=MS.gene36319
chr2.2	mRNA	1940271	1940726	1940271	ID=MS.gene36319.t1;Parent=MS.gene36319
chr2.2	exon	1940271	1940726	1940271	ID=MS.gene36319.t1.exon1;Parent=MS.gene36319.t1
chr2.2	CDS	1940271	1940726	1940271	ID=cds.MS.gene36319.t1;Parent=MS.gene36319.t1

Gene Sequence

>MS.gene36319

ATGTGGCTTCTGCAACTTCCCAGCACGCTCTCAACACCATTTCTCAACGGCAACAATGGCGTCGCCCTCACTCGTCTCTCAAAGCCCACATCCACACTCTCTCTATCCCAGCCCCAATCCCTTCCATCAATCAAAGCTATGAAAACAATGGAAGGAAAAGTTGTGTGTGCCACCAACGACAAAACCGTGTCTGTGGAAGTTACTCGTTTAGCACCTCATCCCAAATACAAGAAACGTATTAGGATGAAGAAGAAGTATCAGGCTCATGATCCTGAAAACGTTTTCAAAGTTGGTGATGTTGTTCAGCTTTGTAAAATTAGACCTATTAGTAAGAATAAAACTTTTCTTGCTGTTCCTGTTCCTGCTAGGAATTCTGGTAAGGCTGATAATTCTTCTGGTGACCTCGACATTCCGCTTCAATCGGAGCAAGAAGAACAGCCTCAGACTCAGGCTTAG

Protein sequence

>MS.gene36319.t1

MWLLQLPSTLSTPFLNGNNGVALTRLSKPTSTLSLSQPQSLPSIKAMKTMEGKVVCATNDKTVSVEVTRLAPHPKYKKRIRMKKKYQAHDPENVFKVGDVVQLCKIRPISKNKTFLAVPVPARNSGKADNSSGDLDIPLQSEQEEQPQTQA