Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055117.t1 | XP_013449050.2 | 93.9 | 179 | 11 | 0 | 1 | 179 | 1 | 179 | 9.50E-91 | 342.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055117.t1 | A0A396H0Q3 | 93.9 | 179 | 11 | 0 | 1 | 179 | 1 | 179 | 6.9e-91 | 342.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055117 | MS.gene30456 | 0.804377 | 2.22E-49 | -1.69E-46 |
| MS.gene055117 | MS.gene46020 | 0.802818 | 4.68E-49 | -1.69E-46 |
| MS.gene055117 | MS.gene46065 | 0.802125 | 6.51E-49 | -1.69E-46 |
| MS.gene055117 | MS.gene78812 | 0.806561 | 7.75E-50 | -1.69E-46 |
| MS.gene055117 | MS.gene94254 | 0.807318 | 5.36E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055117.t1 | MTR_7g066110 | 94.083 | 169 | 10 | 0 | 11 | 179 | 1 | 169 | 3.88e-118 | 332 |
| MS.gene055117.t1 | MTR_4g018860 | 69.822 | 169 | 51 | 0 | 1 | 169 | 1 | 169 | 2.08e-85 | 250 |
| MS.gene055117.t1 | MTR_4g018880 | 67.416 | 178 | 58 | 0 | 1 | 178 | 1 | 178 | 1.21e-83 | 244 |
| MS.gene055117.t1 | MTR_4g018850 | 66.851 | 181 | 58 | 1 | 1 | 179 | 1 | 181 | 2.50e-78 | 233 |
| MS.gene055117.t1 | MTR_4g018880 | 68.939 | 132 | 41 | 0 | 1 | 132 | 1 | 132 | 7.40e-62 | 188 |
| MS.gene055117.t1 | MTR_6g452730 | 49.123 | 171 | 83 | 2 | 7 | 176 | 4 | 171 | 6.10e-55 | 172 |
| MS.gene055117.t1 | MTR_1g101120 | 52.941 | 153 | 71 | 1 | 15 | 166 | 18 | 170 | 3.37e-54 | 171 |
| MS.gene055117.t1 | MTR_7g066090 | 85.526 | 76 | 11 | 0 | 1 | 76 | 1 | 76 | 1.60e-40 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055117.t1 | AT3G54040 | 56.667 | 180 | 72 | 4 | 1 | 178 | 1 | 176 | 6.72e-67 | 202 |
| MS.gene055117.t1 | AT3G54040 | 59.172 | 169 | 63 | 4 | 1 | 167 | 57 | 221 | 2.60e-66 | 203 |
| MS.gene055117.t1 | AT5G52390 | 44.134 | 179 | 99 | 1 | 1 | 178 | 1 | 179 | 6.68e-51 | 162 |
Find 45 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACAATATTGGGGCAATTT+TGG | 0.141377 | 7.4:-38731022 | None:intergenic |
| TGTTGACCTTTACTTCAATT+TGG | 0.213939 | 7.4:+38731040 | MS.gene055117:CDS |
| CTAAAGATACATGGATAAAA+AGG | 0.222715 | 7.4:-38730614 | None:intergenic |
| AAACATAATCAAAGGTAATT+TGG | 0.227054 | 7.4:-38730583 | None:intergenic |
| GAAGTAAAGGTCAACAATAT+TGG | 0.286026 | 7.4:-38731033 | None:intergenic |
| AAGTAAAGGTCAACAATATT+GGG | 0.297379 | 7.4:-38731032 | None:intergenic |
| CTTTACTTCAATTTGGCTGC+TGG | 0.332953 | 7.4:+38731047 | MS.gene055117:CDS |
| TCTTTCTCAATAGCTTCTTC+AGG | 0.363584 | 7.4:+38730786 | MS.gene055117:CDS |
| GTGAGCTCTGGAGCTGCACC+TGG | 0.378232 | 7.4:+38731659 | MS.gene055117:CDS |
| CTTAGGATACTTTGTTTACA+TGG | 0.379493 | 7.4:-38731732 | None:intergenic |
| TCTTGTGACAGAGCAGAAAC+AGG | 0.399775 | 7.4:-38731683 | None:intergenic |
| TTCAGGAAAACGGTGTTTGT+TGG | 0.405418 | 7.4:+38730803 | MS.gene055117:CDS |
| ATGATCGAGCTTGTGAGCTC+TGG | 0.439225 | 7.4:+38731647 | MS.gene055117:CDS |
| TTCAATTTGGCTGCTGGGGA+AGG | 0.450339 | 7.4:+38731053 | MS.gene055117:CDS |
| TTTACTTCAATTTGGCTGCT+GGG | 0.473999 | 7.4:+38731048 | MS.gene055117:CDS |
| CAATAGCTTCTTCAGGAAAA+CGG | 0.477557 | 7.4:+38730793 | MS.gene055117:CDS |
| AGAAGTAGTGGTGGAGCAAA+TGG | 0.478800 | 7.4:+38730875 | MS.gene055117:CDS |
| TACTTTGTTTACATGGGAGA+AGG | 0.498445 | 7.4:-38731725 | None:intergenic |
| CAGCAGCCAAATTGAAGTAA+AGG | 0.504408 | 7.4:-38731046 | None:intergenic |
| GACAGAGCAGAAACAGGGCC+AGG | 0.529854 | 7.4:-38731677 | None:intergenic |
| TTAGGATACTTTGTTTACAT+GGG | 0.551833 | 7.4:-38731731 | None:intergenic |
| ACTTTGTTTACATGGGAGAA+GGG | 0.558007 | 7.4:-38731724 | None:intergenic |
| GCTCGATCATAGCGCGATGA+GGG | 0.572009 | 7.4:-38731634 | None:intergenic |
| CTTTGTTTACATGGGAGAAG+GGG | 0.572016 | 7.4:-38731723 | None:intergenic |
| ACAGAGAAGGCAGCGGATGG+TGG | 0.581266 | 7.4:+38730828 | MS.gene055117:CDS |
| TGTGTGTACCTAAAGATACA+TGG | 0.585474 | 7.4:-38730623 | None:intergenic |
| GTAGTGGTGGAGCAAATGGC+GGG | 0.589388 | 7.4:+38730879 | MS.gene055117:CDS |
| GTGTTTGTTGGAGACAGAGA+AGG | 0.593902 | 7.4:+38730815 | MS.gene055117:CDS |
| TTACTTCAATTTGGCTGCTG+GGG | 0.597417 | 7.4:+38731049 | MS.gene055117:CDS |
| GATCATAGCGCGATGAGGGT+CGG | 0.606329 | 7.4:-38731630 | None:intergenic |
| ATGTAGGACATCAGAAGTAG+TGG | 0.608155 | 7.4:+38730863 | MS.gene055117:CDS |
| CTACTTCTGATGTCCTACAT+TGG | 0.613644 | 7.4:-38730860 | None:intergenic |
| GAGACAGAGAAGGCAGCGGA+TGG | 0.613801 | 7.4:+38730825 | MS.gene055117:CDS |
| AGCTCGATCATAGCGCGATG+AGG | 0.614616 | 7.4:-38731635 | None:intergenic |
| AGTAGTGGTGGAGCAAATGG+CGG | 0.620474 | 7.4:+38730878 | MS.gene055117:CDS |
| TGTGTGTGAGGAGTTGCAAA+AGG | 0.620931 | 7.4:+38730755 | MS.gene055117:CDS |
| GTTGGAGACAGAGAAGGCAG+CGG | 0.628566 | 7.4:+38730821 | MS.gene055117:CDS |
| TAGGACATCAGAAGTAGTGG+TGG | 0.629632 | 7.4:+38730866 | MS.gene055117:CDS |
| CTTGTGACAGAGCAGAAACA+GGG | 0.634654 | 7.4:-38731682 | None:intergenic |
| AGTAAAGGTCAACAATATTG+GGG | 0.636729 | 7.4:-38731031 | None:intergenic |
| GTGGCGTCGAGTACCAATGT+AGG | 0.640711 | 7.4:+38730847 | MS.gene055117:CDS |
| TAATTTGGATGAGAAAGACA+TGG | 0.651318 | 7.4:-38730568 | None:intergenic |
| GATCAATGTGTGAATGCTTG+CGG | 0.665620 | 7.4:+38730915 | MS.gene055117:CDS |
| GACAGCTGAAGTTGTGTGTG+AGG | 0.677896 | 7.4:+38730743 | MS.gene055117:intron |
| TGAATGCTTGCGGAGTTGAG+AGG | 0.714850 | 7.4:+38730925 | MS.gene055117:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAAATAAATAAATTGCAT+AGG | - | chr7.4:38730694-38730713 | None:intergenic | 10.0% |
| !! | AACAAAAAATAAAACAAAGA+AGG | + | chr7.4:38731471-38731490 | MS.gene055117:intron | 15.0% |
| !! | AAGAAATTAAAACAAAAAGA+AGG | - | chr7.4:38731267-38731286 | None:intergenic | 15.0% |
| !! | TAACTTCAAATTTAATCAAA+GGG | + | chr7.4:38731426-38731445 | MS.gene055117:intron | 15.0% |
| !! | TTAACTTCAAATTTAATCAA+AGG | + | chr7.4:38731425-38731444 | MS.gene055117:intron | 15.0% |
| !! | TTTGAACAATTCAAATAATT+GGG | + | chr7.4:38731170-38731189 | MS.gene055117:intron | 15.0% |
| !!! | TTCTTTTTGTTTTAATTTCT+TGG | + | chr7.4:38731266-38731285 | MS.gene055117:intron | 15.0% |
| !!! | TTTTGAACAATTCAAATAAT+TGG | + | chr7.4:38731169-38731188 | MS.gene055117:intron | 15.0% |
| !!! | TTTTTCAAAATAAGATGAAA+AGG | - | chr7.4:38731234-38731253 | None:intergenic | 15.0% |
| !! | AAACATAATCAAAGGTAATT+TGG | - | chr7.4:38730586-38730605 | None:intergenic | 20.0% |
| !! | CTCTCTTGATATTATAATAA+AGG | + | chr7.4:38731546-38731565 | MS.gene055117:intron | 20.0% |
| !! | TAAAGATACATGGATAAAAA+GGG | - | chr7.4:38730616-38730635 | None:intergenic | 20.0% |
| !! | TAATTGGAAAATATATAGCA+GGG | - | chr7.4:38730660-38730679 | None:intergenic | 20.0% |
| !! | TTGTTCAAAATATGATGAAA+AGG | - | chr7.4:38731159-38731178 | None:intergenic | 20.0% |
| !! | TTTCTTGAAGGAAAAAAATA+TGG | + | chr7.4:38731295-38731314 | MS.gene055117:intron | 20.0% |
| ! | AAGTAAAGGTCAACAATATT+GGG | - | chr7.4:38731035-38731054 | None:intergenic | 25.0% |
| ! | ATAGGTAACTAGATAGTAAT+TGG | - | chr7.4:38730676-38730695 | None:intergenic | 25.0% |
| ! | ATCTTCATGCATAAAAGTTA+TGG | - | chr7.4:38731100-38731119 | None:intergenic | 25.0% |
| ! | CTAAAGATACATGGATAAAA+AGG | - | chr7.4:38730617-38730636 | None:intergenic | 25.0% |
| ! | GAACAGAAAAACATAATCAA+AGG | - | chr7.4:38730594-38730613 | None:intergenic | 25.0% |
| ! | GTAATTGGAAAATATATAGC+AGG | - | chr7.4:38730661-38730680 | None:intergenic | 25.0% |
| ! | GTTAAAATGTTAGATCATGA+CGG | - | chr7.4:38731410-38731429 | None:intergenic | 25.0% |
| ! | TTTGCTTAATTACAAAGCAA+TGG | + | chr7.4:38731340-38731359 | MS.gene055117:intron | 25.0% |
| !! | CTTTTTATCCATGTATCTTT+AGG | + | chr7.4:38730615-38730634 | MS.gene055117:CDS | 25.0% |
| AGTAAAGGTCAACAATATTG+GGG | - | chr7.4:38731034-38731053 | None:intergenic | 30.0% | |
| GAAGTAAAGGTCAACAATAT+TGG | - | chr7.4:38731036-38731055 | None:intergenic | 30.0% | |
| TGTTGACCTTTACTTCAATT+TGG | + | chr7.4:38731040-38731059 | MS.gene055117:CDS | 30.0% | |
| ! | ACTTTTATGCATGAAGATCA+TGG | + | chr7.4:38731102-38731121 | MS.gene055117:intron | 30.0% |
| CAATAGCTTCTTCAGGAAAA+CGG | + | chr7.4:38730793-38730812 | MS.gene055117:CDS | 35.0% | |
| CTTTGATACTATGCAAATGC+AGG | + | chr7.4:38731572-38731591 | MS.gene055117:intron | 35.0% | |
| TCTTTCTCAATAGCTTCTTC+AGG | + | chr7.4:38730786-38730805 | MS.gene055117:CDS | 35.0% | |
| TGTGTGTACCTAAAGATACA+TGG | - | chr7.4:38730626-38730645 | None:intergenic | 35.0% | |
| TTTACTTCAATTTGGCTGCT+GGG | + | chr7.4:38731048-38731067 | MS.gene055117:CDS | 35.0% | |
| ! | CAACAATATTGGGGCAATTT+TGG | - | chr7.4:38731025-38731044 | None:intergenic | 35.0% |
| ! | GAAGGTTCTTTTCTTGTAGA+AGG | + | chr7.4:38731489-38731508 | MS.gene055117:intron | 35.0% |
| ! | GTTTTCACCACAAATCACTA+TGG | - | chr7.4:38731196-38731215 | None:intergenic | 35.0% |
| ! | TCTTGGTTGCATTTTCTTGA+AGG | + | chr7.4:38731283-38731302 | MS.gene055117:intron | 35.0% |
| !! | CAAAGTTTTGCTGTGAATTG+TGG | - | chr7.4:38730987-38731006 | None:intergenic | 35.0% |
| AATTGGGCCATAGTGATTTG+TGG | + | chr7.4:38731186-38731205 | MS.gene055117:intron | 40.0% | |
| ATGTAGGACATCAGAAGTAG+TGG | + | chr7.4:38730863-38730882 | MS.gene055117:CDS | 40.0% | |
| CAGCAGCCAAATTGAAGTAA+AGG | - | chr7.4:38731049-38731068 | None:intergenic | 40.0% | |
| CTACTTCTGATGTCCTACAT+TGG | - | chr7.4:38730863-38730882 | None:intergenic | 40.0% | |
| CTTTACTTCAATTTGGCTGC+TGG | + | chr7.4:38731047-38731066 | MS.gene055117:CDS | 40.0% | |
| GATCAATGTGTGAATGCTTG+CGG | + | chr7.4:38730915-38730934 | MS.gene055117:CDS | 40.0% | |
| TTACTTCAATTTGGCTGCTG+GGG | + | chr7.4:38731049-38731068 | MS.gene055117:CDS | 40.0% | |
| TTCAGGAAAACGGTGTTTGT+TGG | + | chr7.4:38730803-38730822 | MS.gene055117:CDS | 40.0% | |
| ! | GGAGTTGAGAGGAATTTTGT+TGG | + | chr7.4:38730936-38730955 | MS.gene055117:CDS | 40.0% |
| ! | TTTTGCATCTCACAAAGGTC+AGG | - | chr7.4:38731608-38731627 | None:intergenic | 40.0% |
| CTTGTGACAGAGCAGAAACA+GGG | - | chr7.4:38731685-38731704 | None:intergenic | 45.0% | |
| TAGGACATCAGAAGTAGTGG+TGG | + | chr7.4:38730866-38730885 | MS.gene055117:CDS | 45.0% | |
| TCTTGTGACAGAGCAGAAAC+AGG | - | chr7.4:38731686-38731705 | None:intergenic | 45.0% | |
| TGTGTGTGAGGAGTTGCAAA+AGG | + | chr7.4:38730755-38730774 | MS.gene055117:CDS | 45.0% | |
| ! | AGAAGTAGTGGTGGAGCAAA+TGG | + | chr7.4:38730875-38730894 | MS.gene055117:CDS | 45.0% |
| ! | CGGTCTTTTGCATCTCACAA+AGG | - | chr7.4:38731613-38731632 | None:intergenic | 45.0% |
| ! | GTGTTTGTTGGAGACAGAGA+AGG | + | chr7.4:38730815-38730834 | MS.gene055117:CDS | 45.0% |
| ATGATCGAGCTTGTGAGCTC+TGG | + | chr7.4:38731647-38731666 | MS.gene055117:CDS | 50.0% | |
| GACAGCTGAAGTTGTGTGTG+AGG | + | chr7.4:38730743-38730762 | MS.gene055117:intron | 50.0% | |
| TGAATGCTTGCGGAGTTGAG+AGG | + | chr7.4:38730925-38730944 | MS.gene055117:CDS | 50.0% | |
| TTCAATTTGGCTGCTGGGGA+AGG | + | chr7.4:38731053-38731072 | MS.gene055117:CDS | 50.0% | |
| ! | AGTAGTGGTGGAGCAAATGG+CGG | + | chr7.4:38730878-38730897 | MS.gene055117:CDS | 50.0% |
| AGCTCGATCATAGCGCGATG+AGG | - | chr7.4:38731638-38731657 | None:intergenic | 55.0% | |
| GATCATAGCGCGATGAGGGT+CGG | - | chr7.4:38731633-38731652 | None:intergenic | 55.0% | |
| GCTCGATCATAGCGCGATGA+GGG | - | chr7.4:38731637-38731656 | None:intergenic | 55.0% | |
| GTTGGAGACAGAGAAGGCAG+CGG | + | chr7.4:38730821-38730840 | MS.gene055117:CDS | 55.0% | |
| ! | GTAGTGGTGGAGCAAATGGC+GGG | + | chr7.4:38730879-38730898 | MS.gene055117:CDS | 55.0% |
| ! | GTGGCGTCGAGTACCAATGT+AGG | + | chr7.4:38730847-38730866 | MS.gene055117:CDS | 55.0% |
| ACAGAGAAGGCAGCGGATGG+TGG | + | chr7.4:38730828-38730847 | MS.gene055117:CDS | 60.0% | |
| GACAGAGCAGAAACAGGGCC+AGG | - | chr7.4:38731680-38731699 | None:intergenic | 60.0% | |
| GAGACAGAGAAGGCAGCGGA+TGG | + | chr7.4:38730825-38730844 | MS.gene055117:CDS | 60.0% | |
| !! | GTGAGCTCTGGAGCTGCACC+TGG | + | chr7.4:38731659-38731678 | MS.gene055117:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 38730570 | 38731739 | 38730570 | ID=MS.gene055117 |
| chr7.4 | mRNA | 38730570 | 38731739 | 38730570 | ID=MS.gene055117.t1;Parent=MS.gene055117 |
| chr7.4 | exon | 38730570 | 38730636 | 38730570 | ID=MS.gene055117.t1.exon1;Parent=MS.gene055117.t1 |
| chr7.4 | CDS | 38730570 | 38730636 | 38730570 | ID=cds.MS.gene055117.t1;Parent=MS.gene055117.t1 |
| chr7.4 | exon | 38730748 | 38731074 | 38730748 | ID=MS.gene055117.t1.exon2;Parent=MS.gene055117.t1 |
| chr7.4 | CDS | 38730748 | 38731074 | 38730748 | ID=cds.MS.gene055117.t1;Parent=MS.gene055117.t1 |
| chr7.4 | exon | 38731594 | 38731739 | 38731594 | ID=MS.gene055117.t1.exon3;Parent=MS.gene055117.t1 |
| chr7.4 | CDS | 38731594 | 38731739 | 38731594 | ID=cds.MS.gene055117.t1;Parent=MS.gene055117.t1 |
| Gene Sequence |
| Protein sequence |
