AlfalfaGEDB | MS.gene94254

M. sativa cultivar XinJiangDaYe / MS.gene94254

KEGG: -
GO: -
PFAM: PF14368.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94254.t1	XP_003623161.2	96.6	117	4	0	1	117	1	117	9.10E-58	232.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94254.t1	G7L5L2	96.6	117	4	0	1	117	1	117	6.5e-58	232.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050082	MS.gene94254	0.851829	6.88E-61	-1.69E-46
MS.gene050170	MS.gene94254	-0.812214	4.74E-51	-1.69E-46
MS.gene050338	MS.gene94254	0.82834	9.49E-55	-1.69E-46
MS.gene050496	MS.gene94254	-0.803962	2.71E-49	-1.69E-46
MS.gene050904	MS.gene94254	-0.848949	4.42E-60	-1.69E-46
MS.gene051138	MS.gene94254	0.806286	8.86E-50	-1.69E-46
MS.gene051651	MS.gene94254	0.82491	6.25E-54	-1.69E-46
MS.gene052606	MS.gene94254	0.804243	2.37E-49	-1.69E-46
MS.gene053420	MS.gene94254	0.80059	1.34E-48	-1.69E-46
MS.gene055117	MS.gene94254	0.807318	5.36E-50	-1.69E-46
MS.gene055158	MS.gene94254	0.902273	1.15E-78	-1.69E-46
MS.gene055159	MS.gene94254	0.809124	2.21E-50	-1.69E-46
MS.gene055748	MS.gene94254	-0.801871	7.34E-49	-1.69E-46
MS.gene055751	MS.gene94254	-0.813087	3.06E-51	-1.69E-46
MS.gene05589	MS.gene94254	-0.824748	6.82E-54	-1.69E-46
MS.gene056387	MS.gene94254	-0.813954	1.97E-51	-1.69E-46
MS.gene056504	MS.gene94254	-0.80681	6.87E-50	-1.69E-46
MS.gene056618	MS.gene94254	0.813696	2.25E-51	-1.69E-46
MS.gene056697	MS.gene94254	0.802812	4.70E-49	-1.69E-46
MS.gene056856	MS.gene94254	0.82493	6.18E-54	-1.69E-46
MS.gene057768	MS.gene94254	0.809358	1.97E-50	-1.69E-46
MS.gene058334	MS.gene94254	0.806331	8.66E-50	-1.69E-46
MS.gene058382	MS.gene94254	0.800031	1.74E-48	-1.69E-46
MS.gene05960	MS.gene94254	0.805068	1.60E-49	-1.69E-46
MS.gene06000	MS.gene94254	-0.833189	6.15E-56	-1.69E-46
MS.gene06042	MS.gene94254	0.817035	4.06E-52	-1.69E-46
MS.gene060823	MS.gene94254	0.829832	4.13E-55	-1.69E-46
MS.gene061044	MS.gene94254	0.827735	1.33E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94254.t1	MTR_7g065160	96.581	117	4	0	1	117	1	117	3.28e-80	231
MS.gene94254.t1	MTR_7g065210	87.179	117	15	0	1	117	1	117	5.24e-73	213
MS.gene94254.t1	MTR_8g070040	45.455	121	61	3	1	117	1	120	3.79e-25	92.0
MS.gene94254.t1	MTR_8g070015	42.105	114	61	3	1	110	1	113	2.11e-19	77.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene94254.t1	AT5G05960	61.261	111	36	2	7	117	13	116	1.03e-42	136
MS.gene94254.t1	AT3G53980	64.211	95	33	1	23	117	21	114	2.62e-42	135
MS.gene94254.t1	AT3G53980	64.211	95	33	1	23	117	21	114	2.62e-42	135
MS.gene94254.t1	AT2G37870	49.107	112	51	4	10	117	6	115	5.67e-26	94.0

Find 21 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTATTGCAGTTCTTGTTGC+AGG	0.361788	7.3:+40774386	MS.gene94254:CDS
AGCTTAAAAGCTTCATTGTC+TGG	0.382047	7.3:-40774455	None:intergenic
TCAAACGTTGCTAAAATGTC+TGG	0.431901	7.3:+40774590	MS.gene94254:CDS
CAGCAAGGTTGCAACGCTTA+GGG	0.465106	7.3:-40774640	None:intergenic
GGATAGTAACAGCAATTTGA+GGG	0.472152	7.3:-40774619	None:intergenic
TCAGCAAGGTTGCAACGCTT+AGG	0.488806	7.3:-40774641	None:intergenic
TGTGCTCAGGTTAAGAAACT+TGG	0.492815	7.3:+40774533	MS.gene94254:CDS
GTGAAATTGTTGTGCTTCGT+TGG	0.500117	7.3:+40774365	MS.gene94254:CDS
GGGATAGTAACAGCAATTTG+AGG	0.508534	7.3:-40774620	None:intergenic
GTCACAAAATTGTTGTGCTC+AGG	0.514410	7.3:+40774520	MS.gene94254:CDS
CGTCCACACTTGTAACCGAC+AGG	0.520932	7.3:-40774668	None:intergenic
CGCCCTGTCGGTTACAAGTG+TGG	0.534054	7.3:+40774665	MS.gene94254:CDS
GTTGAAAGTGCTGGTGAATG+TGG	0.539242	7.3:+40774419	MS.gene94254:CDS
AAAGTGCTGGTGAATGTGGA+AGG	0.549386	7.3:+40774423	MS.gene94254:CDS
AACCTTGCTGATCGCCCTGT+CGG	0.551684	7.3:+40774653	MS.gene94254:CDS
ATTCATAGAGTTGAAAGTGC+TGG	0.554882	7.3:+40774410	MS.gene94254:CDS
ATAACAGCACAAAGACAACT+TGG	0.604458	7.3:-40774563	None:intergenic
AGTGCTGGTGAATGTGGAAG+GGG	0.630405	7.3:+40774425	MS.gene94254:CDS
AACCGACAGGGCGATCAGCA+AGG	0.631783	7.3:-40774655	None:intergenic
AAGTGCTGGTGAATGTGGAA+GGG	0.641454	7.3:+40774424	MS.gene94254:CDS
GTCCACACTTGTAACCGACA+GGG	0.709589	7.3:-40774667	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAATTATTTATCTTTTTTCT+TGG	+	chr7.3:40774945-40774964	MS.gene94254:intron	10.0%
!!!	TACTTATTTGACATTTTTTT+AGG	-	chr7.3:40774907-40774926	None:intergenic	15.0%
	AGCTTAAAAGCTTCATTGTC+TGG	-	chr7.3:40774458-40774477	None:intergenic	35.0%
	ATAACAGCACAAAGACAACT+TGG	-	chr7.3:40774566-40774585	None:intergenic	35.0%
	GGATAGTAACAGCAATTTGA+GGG	-	chr7.3:40774622-40774641	None:intergenic	35.0%
	TACAAAAACGACAGAGTAGT+TGG	-	chr7.3:40774701-40774720	None:intergenic	35.0%
	TCAAACGTTGCTAAAATGTC+TGG	+	chr7.3:40774590-40774609	MS.gene94254:CDS	35.0%
!	ATTCATAGAGTTGAAAGTGC+TGG	+	chr7.3:40774410-40774429	MS.gene94254:CDS	35.0%
	GGGATAGTAACAGCAATTTG+AGG	-	chr7.3:40774623-40774642	None:intergenic	40.0%
	GTCACAAAATTGTTGTGCTC+AGG	+	chr7.3:40774520-40774539	MS.gene94254:CDS	40.0%
	GTGAAATTGTTGTGCTTCGT+TGG	+	chr7.3:40774365-40774384	MS.gene94254:CDS	40.0%
	TGTGCTCAGGTTAAGAAACT+TGG	+	chr7.3:40774533-40774552	MS.gene94254:CDS	40.0%
	GGTATTGCAGTTCTTGTTGC+AGG	+	chr7.3:40774386-40774405	MS.gene94254:CDS	45.0%
!	TCTTTTGCTGCACTTGCACA+AGG	-	chr7.3:40774485-40774504	None:intergenic	45.0%
!!	AAAGTGCTGGTGAATGTGGA+AGG	+	chr7.3:40774423-40774442	MS.gene94254:CDS	45.0%
!!	AAGTGCTGGTGAATGTGGAA+GGG	+	chr7.3:40774424-40774443	MS.gene94254:CDS	45.0%
!!	GTTGAAAGTGCTGGTGAATG+TGG	+	chr7.3:40774419-40774438	MS.gene94254:CDS	45.0%
	CAGCAAGGTTGCAACGCTTA+GGG	-	chr7.3:40774643-40774662	None:intergenic	50.0%
	GTCCACACTTGTAACCGACA+GGG	-	chr7.3:40774670-40774689	None:intergenic	50.0%
	TCAGCAAGGTTGCAACGCTT+AGG	-	chr7.3:40774644-40774663	None:intergenic	50.0%
!!	AGTGCTGGTGAATGTGGAAG+GGG	+	chr7.3:40774425-40774444	MS.gene94254:CDS	50.0%
	AACCTTGCTGATCGCCCTGT+CGG	+	chr7.3:40774653-40774672	MS.gene94254:CDS	55.0%
	CGTCCACACTTGTAACCGAC+AGG	-	chr7.3:40774671-40774690	None:intergenic	55.0%
	AACCGACAGGGCGATCAGCA+AGG	-	chr7.3:40774658-40774677	None:intergenic	60.0%
!	CGCCCTGTCGGTTACAAGTG+TGG	+	chr7.3:40774665-40774684	MS.gene94254:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	40774353	40775031	40774353	ID=MS.gene94254
chr7.3	mRNA	40774353	40775031	40774353	ID=MS.gene94254.t1;Parent=MS.gene94254
chr7.3	exon	40774353	40774689	40774353	ID=MS.gene94254.t1.exon1;Parent=MS.gene94254.t1
chr7.3	CDS	40774353	40774689	40774353	ID=cds.MS.gene94254.t1;Parent=MS.gene94254.t1
chr7.3	exon	40775015	40775031	40775015	ID=MS.gene94254.t1.exon2;Parent=MS.gene94254.t1
chr7.3	CDS	40775015	40775031	40775015	ID=cds.MS.gene94254.t1;Parent=MS.gene94254.t1

Gene Sequence

>MS.gene94254

ATGGAGGGTCTTGTGAAATTGTTGTGCTTCGTTGGTATTGCAGTTCTTGTTGCAGGAATTCATAGAGTTGAAAGTGCTGGTGAATGTGGAAGGGGTACTACACCAGACAATGAAGCTTTTAAGCTAATTCCTTGTGCAAGTGCAGCAAAAGATGAAAATGCTAGTGTGTCACAAAATTGTTGTGCTCAGGTTAAGAAACTTGGTCAGAATCCAAGTTGTCTTTGTGCTGTTATGCTTTCAAACGTTGCTAAAATGTCTGGTGCTAACCCTCAAATTGCTGTTACTATCCCTAAGCGTTGCAACCTTGCTGATCGCCCTGTCGGTTACAAGTGTGGACGTAAGTTACCAACTACTCTGTCGTTTTTGTAAAAAAGTTGTTCGTATTTATTTGTTGTTTTCAAAGCTCAATGTTAGAGAAATAAGTGAACTAAAACTATAAATAGTTAAGAGTATTATAGAAAATAGATAAATAACTCTATCAAAAGTAAAAAAAAAAAAAATAATTTTGCTAAAATCTACTTTCTTTTTATAGAACTAAAAACATATTTATGCCTAAAAAAATGTCAAATAAGTATGCTGCTAATATATGTTTTAATTATTTATCTTTTTTCTTGGTAGATCAATATGCTAGCTAATTCTTTTCACAATCTCTTATATTGCAGCTTACACTCTTCCTTGA

Protein sequence

>MS.gene94254.t1

MEGLVKLLCFVGIAVLVAGIHRVESAGECGRGTTPDNEAFKLIPCASAAKDENASVSQNCCAQVKKLGQNPSCLCAVMLSNVAKMSGANPQIAVTIPKRCNLADRPVGYKCGPYTLP