Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46065.t1 | XP_003623166.1 | 93.2 | 117 | 8 | 0 | 1 | 117 | 1 | 117 | 2.90E-56 | 227.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46065.t1 | G7L5L7 | 93.2 | 117 | 8 | 0 | 1 | 117 | 1 | 117 | 2.1e-56 | 227.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050082 | MS.gene46065 | 0.838862 | 2.23E-57 | -1.69E-46 |
| MS.gene050338 | MS.gene46065 | 0.831612 | 1.51E-55 | -1.69E-46 |
| MS.gene050444 | MS.gene46065 | -0.802518 | 5.40E-49 | -1.69E-46 |
| MS.gene050904 | MS.gene46065 | -0.838834 | 2.27E-57 | -1.69E-46 |
| MS.gene051138 | MS.gene46065 | 0.820543 | 6.49E-53 | -1.69E-46 |
| MS.gene051364 | MS.gene46065 | 0.800462 | 1.43E-48 | -1.69E-46 |
| MS.gene051651 | MS.gene46065 | 0.823144 | 1.62E-53 | -1.69E-46 |
| MS.gene052129 | MS.gene46065 | 0.820376 | 7.09E-53 | -1.69E-46 |
| MS.gene052606 | MS.gene46065 | 0.808305 | 3.31E-50 | -1.69E-46 |
| MS.gene053108 | MS.gene46065 | 0.800755 | 1.24E-48 | -1.69E-46 |
| MS.gene053776 | MS.gene46065 | 0.811358 | 7.29E-51 | -1.69E-46 |
| MS.gene055117 | MS.gene46065 | 0.802125 | 6.51E-49 | -1.69E-46 |
| MS.gene055158 | MS.gene46065 | 0.91179 | 4.08E-83 | -1.69E-46 |
| MS.gene055159 | MS.gene46065 | 0.80717 | 5.76E-50 | -1.69E-46 |
| MS.gene055748 | MS.gene46065 | -0.817946 | 2.53E-52 | -1.69E-46 |
| MS.gene055750 | MS.gene46065 | -0.802561 | 5.29E-49 | -1.69E-46 |
| MS.gene055751 | MS.gene46065 | -0.825181 | 5.39E-54 | -1.69E-46 |
| MS.gene05589 | MS.gene46065 | -0.815658 | 8.26E-52 | -1.69E-46 |
| MS.gene056283 | MS.gene46065 | -0.802945 | 4.41E-49 | -1.69E-46 |
| MS.gene056387 | MS.gene46065 | -0.811004 | 8.69E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene46065 | -0.807106 | 5.94E-50 | -1.69E-46 |
| MS.gene056618 | MS.gene46065 | 0.810993 | 8.74E-51 | -1.69E-46 |
| MS.gene056856 | MS.gene46065 | 0.833904 | 4.08E-56 | -1.69E-46 |
| MS.gene057768 | MS.gene46065 | 0.813491 | 2.49E-51 | -1.69E-46 |
| MS.gene058334 | MS.gene46065 | 0.803089 | 4.12E-49 | -1.69E-46 |
| MS.gene059526 | MS.gene46065 | 0.806917 | 6.52E-50 | -1.69E-46 |
| MS.gene05960 | MS.gene46065 | 0.806461 | 8.13E-50 | -1.69E-46 |
| MS.gene06000 | MS.gene46065 | -0.821164 | 4.67E-53 | -1.69E-46 |
| MS.gene060762 | MS.gene46065 | -0.809534 | 1.80E-50 | -1.69E-46 |
| MS.gene060823 | MS.gene46065 | 0.833533 | 5.05E-56 | -1.69E-46 |
| MS.gene060982 | MS.gene46065 | -0.802696 | 4.96E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46065.t1 | MTR_7g065210 | 93.162 | 117 | 8 | 0 | 1 | 117 | 1 | 117 | 1.01e-78 | 227 |
| MS.gene46065.t1 | MTR_7g065160 | 88.889 | 117 | 13 | 0 | 1 | 117 | 1 | 117 | 8.18e-75 | 217 |
| MS.gene46065.t1 | MTR_8g070040 | 45.455 | 121 | 61 | 3 | 1 | 117 | 1 | 120 | 9.40e-26 | 93.6 |
| MS.gene46065.t1 | MTR_8g070015 | 42.105 | 114 | 61 | 3 | 1 | 110 | 1 | 113 | 5.03e-20 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46065.t1 | AT3G53980 | 68.421 | 95 | 29 | 1 | 23 | 117 | 21 | 114 | 2.24e-44 | 140 |
| MS.gene46065.t1 | AT3G53980 | 68.421 | 95 | 29 | 1 | 23 | 117 | 21 | 114 | 2.24e-44 | 140 |
| MS.gene46065.t1 | AT5G05960 | 58.120 | 117 | 44 | 2 | 5 | 117 | 1 | 116 | 8.88e-42 | 134 |
| MS.gene46065.t1 | AT2G37870 | 44.144 | 111 | 60 | 2 | 8 | 117 | 6 | 115 | 2.07e-25 | 92.8 |
Find 24 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTAAATGCTTCCATATC+TGG | 0.265044 | 7.2:+42683995 | None:intergenic |
| TAGCAAAGTTGCAGCGTTTA+GGG | 0.296288 | 7.2:+42683810 | None:intergenic |
| GGTATTGCAGTTCTTGTTGC+AGG | 0.317135 | 7.2:-42684064 | MS.gene46065:CDS |
| TTAGCAAAGTTGCAGCGTTT+AGG | 0.351023 | 7.2:+42683809 | None:intergenic |
| CGTCCACACTTATAACCAAT+GGG | 0.425846 | 7.2:+42683782 | None:intergenic |
| TCAAACGTTGCTAAAATGTC+TGG | 0.462464 | 7.2:-42683860 | MS.gene46065:CDS |
| AAGGGGTACTACACCAGATA+TGG | 0.470603 | 7.2:-42684008 | MS.gene46065:CDS |
| GGATAGTAACAGCAATTTGA+GGG | 0.472152 | 7.2:+42683831 | None:intergenic |
| GGGATAGTAACAGCAATTTG+AGG | 0.508534 | 7.2:+42683830 | None:intergenic |
| CGAGGCAAAACAATTTCATA+AGG | 0.511907 | 7.2:+42684089 | None:intergenic |
| GTATTGCAGTTCTTGTTGCA+GGG | 0.512563 | 7.2:-42684063 | MS.gene46065:CDS |
| GTTGAAAGTGCAGGTGAATG+TGG | 0.515102 | 7.2:-42684031 | MS.gene46065:CDS |
| AACTTTGCTAATCGCCCCAT+TGG | 0.518869 | 7.2:-42683797 | MS.gene46065:CDS |
| ACGTCCACACTTATAACCAA+TGG | 0.527913 | 7.2:+42683781 | None:intergenic |
| TCTTGTGCTGCACTTGCACA+AGG | 0.541464 | 7.2:+42683968 | None:intergenic |
| AAAGTGCAGGTGAATGTGGA+AGG | 0.549386 | 7.2:-42684027 | MS.gene46065:CDS |
| CGCCCCATTGGTTATAAGTG+TGG | 0.571913 | 7.2:-42683785 | MS.gene46065:CDS |
| TGTGATCAGACTAAGAAACT+TGG | 0.574065 | 7.2:-42683917 | MS.gene46065:CDS |
| AAAACTGCACAAAGACAACT+TGG | 0.590774 | 7.2:+42683887 | None:intergenic |
| ATTCATAGAGTTGAAAGTGC+AGG | 0.591870 | 7.2:-42684040 | MS.gene46065:CDS |
| AGTGCAGGTGAATGTGGAAG+GGG | 0.630405 | 7.2:-42684025 | MS.gene46065:CDS |
| AAGTGCAGGTGAATGTGGAA+GGG | 0.641454 | 7.2:-42684026 | MS.gene46065:CDS |
| CAAGAACTGCAATACCAACG+AGG | 0.744882 | 7.2:+42684071 | None:intergenic |
| GTCCACACTTATAACCAATG+GGG | 0.748420 | 7.2:+42683783 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATAACTTTTATATGAAAA+CGG | + | chr7.2:42683802-42683821 | None:intergenic | 10.0% |
| !!! | TTTTAACAATAAATTTTCTT+AGG | + | chr7.2:42683929-42683948 | None:intergenic | 10.0% |
| !! | ATGTAACATTAATAATTGAT+AGG | - | chr7.2:42683854-42683873 | MS.gene46065:CDS | 15.0% |
| !!! | GTTTTCATATAAAAGTTATT+TGG | - | chr7.2:42683801-42683820 | MS.gene46065:CDS | 15.0% |
| !! | AATGAGATTATAGATAGTTA+AGG | - | chr7.2:42683880-42683899 | MS.gene46065:CDS | 20.0% |
| !! | ATGAGATTATAGATAGTTAA+GGG | - | chr7.2:42683881-42683900 | MS.gene46065:CDS | 20.0% |
| !!! | AAATCAGAATTTTATAAGAG+TGG | + | chr7.2:42684078-42684097 | None:intergenic | 20.0% |
| ! | TTGTTAAAATCTTCCCTTTA+TGG | - | chr7.2:42683940-42683959 | MS.gene46065:CDS | 25.0% |
| !! | CTATTTGTCAATTTTCTTGA+TGG | - | chr7.2:42684039-42684058 | MS.gene46065:CDS | 25.0% |
| !!! | TAGATAGTTAAGGGTATTAT+AGG | - | chr7.2:42683890-42683909 | MS.gene46065:CDS | 25.0% |
| TGCCTAAGAAAAATGTGAAA+CGG | - | chr7.2:42683987-42684006 | MS.gene46065:CDS | 30.0% | |
| ! | TACCGTTTCACATTTTTCTT+AGG | + | chr7.2:42683992-42684011 | None:intergenic | 30.0% |
| !! | CTAGTTTCTAGTTCCATAAA+GGG | + | chr7.2:42683956-42683975 | None:intergenic | 30.0% |
| !! | TCTAGTTTCTAGTTCCATAA+AGG | + | chr7.2:42683957-42683976 | None:intergenic | 30.0% |
| AAAACTGCACAAAGACAACT+TGG | + | chr7.2:42683637-42683656 | None:intergenic | 35.0% | |
| AGCTTAAATGCTTCCATATC+TGG | + | chr7.2:42683529-42683548 | None:intergenic | 35.0% | |
| CGAGGCAAAACAATTTCATA+AGG | + | chr7.2:42683435-42683454 | None:intergenic | 35.0% | |
| GGATAGTAACAGCAATTTGA+GGG | + | chr7.2:42683693-42683712 | None:intergenic | 35.0% | |
| TAAGTAACTAACAAGTCCCT+CGG | - | chr7.2:42683762-42683781 | MS.gene46065:intron | 35.0% | |
| TCAAACGTTGCTAAAATGTC+TGG | - | chr7.2:42683661-42683680 | MS.gene46065:intron | 35.0% | |
| TGTGATCAGACTAAGAAACT+TGG | - | chr7.2:42683604-42683623 | MS.gene46065:intron | 35.0% | |
| ! | ATTCATAGAGTTGAAAGTGC+AGG | - | chr7.2:42683481-42683500 | MS.gene46065:intron | 35.0% |
| !! | ATGAAATTGTTTTGCCTCGT+TGG | - | chr7.2:42683436-42683455 | MS.gene46065:intron | 35.0% |
| ACGTCCACACTTATAACCAA+TGG | + | chr7.2:42683743-42683762 | None:intergenic | 40.0% | |
| CGTCCACACTTATAACCAAT+GGG | + | chr7.2:42683742-42683761 | None:intergenic | 40.0% | |
| GGCAACTTAAAAGAAACCGA+GGG | + | chr7.2:42683781-42683800 | None:intergenic | 40.0% | |
| GGGATAGTAACAGCAATTTG+AGG | + | chr7.2:42683694-42683713 | None:intergenic | 40.0% | |
| GTATTGCAGTTCTTGTTGCA+GGG | - | chr7.2:42683458-42683477 | MS.gene46065:intron | 40.0% | |
| GTCCACACTTATAACCAATG+GGG | + | chr7.2:42683741-42683760 | None:intergenic | 40.0% | |
| TAGCAAAGTTGCAGCGTTTA+GGG | + | chr7.2:42683714-42683733 | None:intergenic | 40.0% | |
| TTAGCAAAGTTGCAGCGTTT+AGG | + | chr7.2:42683715-42683734 | None:intergenic | 40.0% | |
| AAAGTGCAGGTGAATGTGGA+AGG | - | chr7.2:42683494-42683513 | MS.gene46065:intron | 45.0% | |
| AACTTTGCTAATCGCCCCAT+TGG | - | chr7.2:42683724-42683743 | MS.gene46065:intron | 45.0% | |
| AAGTGCAGGTGAATGTGGAA+GGG | - | chr7.2:42683495-42683514 | MS.gene46065:intron | 45.0% | |
| CAAGAACTGCAATACCAACG+AGG | + | chr7.2:42683453-42683472 | None:intergenic | 45.0% | |
| CGGCAACTTAAAAGAAACCG+AGG | + | chr7.2:42683782-42683801 | None:intergenic | 45.0% | |
| GGTATTGCAGTTCTTGTTGC+AGG | - | chr7.2:42683457-42683476 | MS.gene46065:intron | 45.0% | |
| ! | GTTGAAAGTGCAGGTGAATG+TGG | - | chr7.2:42683490-42683509 | MS.gene46065:intron | 45.0% |
| !! | AAGGGGTACTACACCAGATA+TGG | - | chr7.2:42683513-42683532 | MS.gene46065:intron | 45.0% |
| AGTGCAGGTGAATGTGGAAG+GGG | - | chr7.2:42683496-42683515 | MS.gene46065:intron | 50.0% | |
| TCTTGTGCTGCACTTGCACA+AGG | + | chr7.2:42683556-42683575 | None:intergenic | 50.0% | |
| ! | CGCCCCATTGGTTATAAGTG+TGG | - | chr7.2:42683736-42683755 | MS.gene46065:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 42683424 | 42684119 | 42683424 | ID=MS.gene46065 |
| chr7.2 | mRNA | 42683424 | 42684119 | 42683424 | ID=MS.gene46065.t1;Parent=MS.gene46065 |
| chr7.2 | exon | 42683783 | 42684119 | 42683783 | ID=MS.gene46065.t1.exon1;Parent=MS.gene46065.t1 |
| chr7.2 | CDS | 42683783 | 42684119 | 42683783 | ID=cds.MS.gene46065.t1;Parent=MS.gene46065.t1 |
| chr7.2 | exon | 42683424 | 42683440 | 42683424 | ID=MS.gene46065.t1.exon2;Parent=MS.gene46065.t1 |
| chr7.2 | CDS | 42683424 | 42683440 | 42683424 | ID=cds.MS.gene46065.t1;Parent=MS.gene46065.t1 |
| Gene Sequence |
| Protein sequence |
