AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09712


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09712.t1 MTR_5g013440 93.678 174 11 0 18 191 79 252 1.57e-122 346
MS.gene09712.t1 MTR_4g099370 69.767 172 52 0 20 191 80 251 3.24e-93 272
MS.gene09712.t1 MTR_4g099400 57.738 168 71 0 22 189 11 178 1.67e-75 224
MS.gene09712.t1 MTR_4g075790 37.278 169 105 1 22 190 10 177 2.88e-41 138
MS.gene09712.t1 MTR_4g075790 37.278 169 105 1 22 190 76 243 3.78e-40 137
MS.gene09712.t1 MTR_4g098930 38.857 175 106 1 18 191 79 253 1.26e-39 135
MS.gene09712.t1 MTR_2g090265 28.161 174 116 5 22 191 89 257 1.22e-18 81.3
MS.gene09712.t1 MTR_2g090270 26.705 176 120 5 19 190 88 258 1.26e-17 78.6
MS.gene09712.t1 MTR_4g099380 34.932 146 75 5 52 191 1 132 4.84e-17 77.4
MS.gene09712.t1 MTR_0809s0010 30.114 176 116 5 18 190 99 270 2.18e-16 75.5
MS.gene09712.t1 MTR_4g028640 27.273 176 120 6 18 190 94 264 4.37e-16 74.3
MS.gene09712.t1 MTR_0122s0020 26.554 177 121 5 18 190 93 264 1.79e-15 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09712.t1 AT4G17030 57.396 169 71 1 20 188 80 247 2.78e-74 224
MS.gene09712.t1 AT4G38400 37.647 170 105 1 22 190 79 248 5.25e-37 129
MS.gene09712.t1 AT3G45970 36.842 171 106 2 22 190 51 221 2.16e-36 127
MS.gene09712.t1 AT3G45960 34.706 170 110 1 22 190 30 199 3.69e-36 125
MS.gene09712.t1 AT3G45970 36.842 171 106 2 22 190 78 248 3.97e-36 127
MS.gene09712.t1 AT3G45960 34.706 170 110 1 22 190 78 247 1.18e-35 125
MS.gene09712.t1 AT1G65680 28.814 177 116 6 20 190 101 273 8.25e-18 79.3
MS.gene09712.t1 AT4G28250 26.705 176 121 6 20 191 90 261 6.36e-14 68.6
MS.gene09712.t1 AT5G05290 26.316 190 126 7 3 185 69 251 1.01e-11 62.4

Find 55 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCCGTGGCTATTCAAATT+TGG 0.158342 5.2:-5405855 MS.gene09712:CDS
TGGCTATAATATCTTGTTTA+AGG 0.174105 5.2:-5405750 MS.gene09712:CDS
TACGCATTGGCCTCCATTCT+TGG 0.310288 5.2:+5405541 None:intergenic
ATCTCTAGTGATTGGGAAGC+TGG 0.319429 5.2:-5405408 MS.gene09712:CDS
GTGGTAGTGCTGGGCTGTAT+TGG 0.321854 5.2:-5405449 MS.gene09712:CDS
CCATTCTCGTCACACAATTC+TGG 0.326522 5.2:+5405935 None:intergenic
TGGTAGTGCTGGGCTGTATT+GGG 0.329472 5.2:-5405448 MS.gene09712:CDS
CCCCGTGGCTATTCAAATTT+GGG 0.333249 5.2:-5405854 MS.gene09712:CDS
AGGCCAATGCGTAGGGCATT+TGG 0.356145 5.2:-5405531 MS.gene09712:CDS
CCGATTTCGGTGTGGGAGAT+AGG 0.376563 5.2:-5405895 MS.gene09712:CDS
CCTATCTCCCACACCGAAAT+CGG 0.384864 5.2:+5405895 None:intergenic
AGACTGTTATCTCTAGTGAT+TGG 0.400920 5.2:-5405416 MS.gene09712:CDS
GCCATTCTTATTCTCTATGT+TGG 0.402224 5.2:-5405695 MS.gene09712:CDS
CTCCAAGTGAGTGGTAGTGC+TGG 0.409157 5.2:-5405459 MS.gene09712:CDS
GACTGTTATCTCTAGTGATT+GGG 0.436426 5.2:-5405415 MS.gene09712:CDS
AACAGGAAGATTGCCAAGAA+TGG 0.466268 5.2:-5405554 MS.gene09712:intron
ATTCTTATTCTCTATGTTGG+TGG 0.472042 5.2:-5405692 MS.gene09712:CDS
TCTCTAGTGATTGGGAAGCT+GGG 0.472781 5.2:-5405407 MS.gene09712:CDS
TATGTGGTGGTGACCGATTT+CGG 0.479504 5.2:-5405908 MS.gene09712:CDS
TCGCAGGCGTTTCTTCGCTA+TGG 0.490569 5.2:-5406196 MS.gene09712:CDS
TCGCTATGGAAGAATGGATC+TGG 0.490619 5.2:-5406182 MS.gene09712:CDS
CCAGAATTGTGTGACGAGAA+TGG 0.501363 5.2:-5405935 MS.gene09712:CDS
ATCACTAGAGATAACAGTCT+TGG 0.514327 5.2:+5405419 None:intergenic
CTCTAGTGATTGGGAAGCTG+GGG 0.520412 5.2:-5405406 MS.gene09712:CDS
AACTTGATATCACCTCTTGG+AGG 0.525176 5.2:+5405486 None:intergenic
CTCAACTTGATATCACCTCT+TGG 0.527086 5.2:+5405483 None:intergenic
TTATAGCCATTGTATTTGCA+AGG 0.532628 5.2:+5405764 None:intergenic
GCCCAGCACTACCACTCACT+TGG 0.534848 5.2:+5405457 None:intergenic
ACCAACATAGAGAATAAGAA+TGG 0.537864 5.2:+5405694 None:intergenic
GCCCAAATTTGAATAGCCAC+GGG 0.544992 5.2:+5405853 None:intergenic
TGCCCAAATTTGAATAGCCA+CGG 0.546821 5.2:+5405852 None:intergenic
GTTCCAAATGCCCTACGCAT+TGG 0.550110 5.2:+5405528 None:intergenic
GTAAATGATGGCAATGTCGC+AGG 0.552094 5.2:-5406212 None:intergenic
TCCAAGTGAGTGGTAGTGCT+GGG 0.552324 5.2:-5405458 MS.gene09712:CDS
GTTTCTTCGCTATGGAAGAA+TGG 0.552967 5.2:-5406188 MS.gene09712:CDS
AAGAATGGAGGCCAATGCGT+AGG 0.554025 5.2:-5405539 MS.gene09712:CDS
TGGAAGAATGGATCTGGTTG+TGG 0.555017 5.2:-5406176 MS.gene09712:CDS
TATAGCCATTGTATTTGCAA+GGG 0.561411 5.2:+5405765 None:intergenic
GTGGTGACCGATTTCGGTGT+GGG 0.566642 5.2:-5405902 MS.gene09712:CDS
AACTGCTGTTCAGTTGTGGC+AGG 0.569473 5.2:-5405657 MS.gene09712:intron
ATAAGAATGGCTAAGTAGTG+AGG 0.575706 5.2:+5405707 None:intergenic
ACTTGATATCACCTCTTGGA+GGG 0.580063 5.2:+5405487 None:intergenic
AGAATGGAGGCCAATGCGTA+GGG 0.588746 5.2:-5405538 MS.gene09712:CDS
TAGTGCTGGGCTGTATTGGG+TGG 0.610771 5.2:-5405445 MS.gene09712:CDS
AGGAAGATTGCCAAGAATGG+AGG 0.614849 5.2:-5405551 MS.gene09712:CDS
TGACGAGAATGGAGCATATG+TGG 0.619740 5.2:-5405924 MS.gene09712:CDS
AGAATCCCTTGCAAATACAA+TGG 0.620915 5.2:-5405770 MS.gene09712:CDS
ATGTAACTGCTGTTCAGTTG+TGG 0.651186 5.2:-5405661 MS.gene09712:CDS
GGTGGTGACCGATTTCGGTG+TGG 0.653687 5.2:-5405903 MS.gene09712:CDS
AAGTTGAGGCTCCAAGTGAG+TGG 0.655665 5.2:-5405468 MS.gene09712:CDS
CAAGAGGTGATATCAAGTTG+AGG 0.672009 5.2:-5405482 MS.gene09712:CDS
GACTTCATCATGAGTCCCCG+TGG 0.688519 5.2:-5405869 MS.gene09712:CDS
GATGCAGAGAACCCTCCAAG+AGG 0.690257 5.2:-5405498 MS.gene09712:CDS
CCCAAATTTGAATAGCCACG+GGG 0.693150 5.2:+5405854 None:intergenic
CGAGAATGGAGCATATGTGG+TGG 0.757236 5.2:-5405921 MS.gene09712:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAAATAATGATGAAAT+AGG - chr5.2:5405610-5405629 MS.gene09712:intron 10.0%
!! TGAAGTAATATATATTATAA+AGG - chr5.2:5405543-5405562 MS.gene09712:CDS 10.0%
!! TATTAACAATCTCAAAATAA+AGG + chr5.2:5405955-5405974 None:intergenic 15.0%
!! ACATTTCTTGAAATTGAATT+GGG - chr5.2:5405985-5406004 MS.gene09712:intron 20.0%
!! TACATTTCTTGAAATTGAAT+TGG - chr5.2:5405984-5406003 MS.gene09712:intron 20.0%
! AAATAATGATGAAATAGGTG+AGG - chr5.2:5405615-5405634 MS.gene09712:intron 25.0%
! TATTCAAATTTGGGCAAAAA+TGG - chr5.2:5405737-5405756 MS.gene09712:CDS 25.0%
!! AACTAATTGTTAGTTGATGT+TGG + chr5.2:5405579-5405598 None:intergenic 25.0%
!! AGAACTTTTTAAATATGGTG+TGG - chr5.2:5405772-5405791 MS.gene09712:CDS 25.0%
!! TCTGCAGAACTTTTTAAATA+TGG - chr5.2:5405767-5405786 MS.gene09712:CDS 25.0%
!! TGGCTATAATATCTTGTTTA+AGG - chr5.2:5405832-5405851 MS.gene09712:CDS 25.0%
ACCAACATAGAGAATAAGAA+TGG + chr5.2:5405891-5405910 None:intergenic 30.0%
ATTCTTATTCTCTATGTTGG+TGG - chr5.2:5405890-5405909 MS.gene09712:CDS 30.0%
TATAGCCATTGTATTTGCAA+GGG + chr5.2:5405820-5405839 None:intergenic 30.0%
TGAATTGGGTTTACTTGAAA+TGG - chr5.2:5405999-5406018 MS.gene09712:intron 30.0%
TTATAGCCATTGTATTTGCA+AGG + chr5.2:5405821-5405840 None:intergenic 30.0%
! ACTTTTTAAATATGGTGTGG+TGG - chr5.2:5405775-5405794 MS.gene09712:CDS 30.0%
AGAATCCCTTGCAAATACAA+TGG - chr5.2:5405812-5405831 MS.gene09712:CDS 35.0%
AGACTGTTATCTCTAGTGAT+TGG - chr5.2:5406166-5406185 MS.gene09712:CDS 35.0%
ATAAGAATGGCTAAGTAGTG+AGG + chr5.2:5405878-5405897 None:intergenic 35.0%
ATCACTAGAGATAACAGTCT+TGG + chr5.2:5406166-5406185 None:intergenic 35.0%
GACTGTTATCTCTAGTGATT+GGG - chr5.2:5406167-5406186 MS.gene09712:CDS 35.0%
GCCATTCTTATTCTCTATGT+TGG - chr5.2:5405887-5405906 MS.gene09712:CDS 35.0%
GGGTTTACTTGAAATGGTAA+TGG - chr5.2:5406005-5406024 MS.gene09712:intron 35.0%
!! ACTTGAAATGGTAATGGAAC+AGG - chr5.2:5406011-5406030 MS.gene09712:intron 35.0%
AACAGGAAGATTGCCAAGAA+TGG - chr5.2:5406028-5406047 MS.gene09712:intron 40.0%
AACTTGATATCACCTCTTGG+AGG + chr5.2:5406099-5406118 None:intergenic 40.0%
ACTTGATATCACCTCTTGGA+GGG + chr5.2:5406098-5406117 None:intergenic 40.0%
ATGTAACTGCTGTTCAGTTG+TGG - chr5.2:5405921-5405940 MS.gene09712:CDS 40.0%
CTCAACTTGATATCACCTCT+TGG + chr5.2:5406102-5406121 None:intergenic 40.0%
GTTTCTTCGCTATGGAAGAA+TGG - chr5.2:5405394-5405413 MS.gene09712:CDS 40.0%
TGCCCAAATTTGAATAGCCA+CGG + chr5.2:5405733-5405752 None:intergenic 40.0%
! CAAGAGGTGATATCAAGTTG+AGG - chr5.2:5406100-5406119 MS.gene09712:intron 40.0%
AGGAAGATTGCCAAGAATGG+AGG - chr5.2:5406031-5406050 MS.gene09712:intron 45.0%
ATCTCTAGTGATTGGGAAGC+TGG - chr5.2:5406174-5406193 MS.gene09712:CDS 45.0%
CCAGAATTGTGTGACGAGAA+TGG - chr5.2:5405647-5405666 MS.gene09712:intron 45.0%
CCATTCTCGTCACACAATTC+TGG + chr5.2:5405650-5405669 None:intergenic 45.0%
CCCAAATTTGAATAGCCACG+GGG + chr5.2:5405731-5405750 None:intergenic 45.0%
CCCCGTGGCTATTCAAATTT+GGG - chr5.2:5405728-5405747 MS.gene09712:CDS 45.0%
GCCCAAATTTGAATAGCCAC+GGG + chr5.2:5405732-5405751 None:intergenic 45.0%
TCCCCGTGGCTATTCAAATT+TGG - chr5.2:5405727-5405746 MS.gene09712:CDS 45.0%
TCGCTATGGAAGAATGGATC+TGG - chr5.2:5405400-5405419 MS.gene09712:CDS 45.0%
TCTCTAGTGATTGGGAAGCT+GGG - chr5.2:5406175-5406194 MS.gene09712:CDS 45.0%
TGACGAGAATGGAGCATATG+TGG - chr5.2:5405658-5405677 MS.gene09712:CDS 45.0%
TGGAAGAATGGATCTGGTTG+TGG - chr5.2:5405406-5405425 MS.gene09712:CDS 45.0%
! GAGGATTTTTGCTGCGTTCA+TGG + chr5.2:5405859-5405878 None:intergenic 45.0%
! TATGTGGTGGTGACCGATTT+CGG - chr5.2:5405674-5405693 MS.gene09712:CDS 45.0%
AACTGCTGTTCAGTTGTGGC+AGG - chr5.2:5405925-5405944 MS.gene09712:CDS 50.0%
AAGAATGGAGGCCAATGCGT+AGG - chr5.2:5406043-5406062 MS.gene09712:intron 50.0%
AAGTTGAGGCTCCAAGTGAG+TGG - chr5.2:5406114-5406133 MS.gene09712:intron 50.0%
AGAATGGAGGCCAATGCGTA+GGG - chr5.2:5406044-5406063 MS.gene09712:intron 50.0%
CCTATCTCCCACACCGAAAT+CGG + chr5.2:5405690-5405709 None:intergenic 50.0%
CGAGAATGGAGCATATGTGG+TGG - chr5.2:5405661-5405680 MS.gene09712:CDS 50.0%
CTCTAGTGATTGGGAAGCTG+GGG - chr5.2:5406176-5406195 MS.gene09712:CDS 50.0%
GTTCCAAATGCCCTACGCAT+TGG + chr5.2:5406057-5406076 None:intergenic 50.0%
! TACGCATTGGCCTCCATTCT+TGG + chr5.2:5406044-5406063 None:intergenic 50.0%
! TCCAAGTGAGTGGTAGTGCT+GGG - chr5.2:5406124-5406143 MS.gene09712:intron 50.0%
! TGGTAGTGCTGGGCTGTATT+GGG - chr5.2:5406134-5406153 MS.gene09712:intron 50.0%
GACTTCATCATGAGTCCCCG+TGG - chr5.2:5405713-5405732 MS.gene09712:CDS 55.0%
GATGCAGAGAACCCTCCAAG+AGG - chr5.2:5406084-5406103 MS.gene09712:intron 55.0%
TCGCAGGCGTTTCTTCGCTA+TGG - chr5.2:5405386-5405405 MS.gene09712:CDS 55.0%
! AGGCCAATGCGTAGGGCATT+TGG - chr5.2:5406051-5406070 MS.gene09712:intron 55.0%
! CCGATTTCGGTGTGGGAGAT+AGG - chr5.2:5405687-5405706 MS.gene09712:CDS 55.0%
! CTCCAAGTGAGTGGTAGTGC+TGG - chr5.2:5406123-5406142 MS.gene09712:intron 55.0%
! GTGGTAGTGCTGGGCTGTAT+TGG - chr5.2:5406133-5406152 MS.gene09712:intron 55.0%
! GTGGTGACCGATTTCGGTGT+GGG - chr5.2:5405680-5405699 MS.gene09712:CDS 55.0%
! TAGTGCTGGGCTGTATTGGG+TGG - chr5.2:5406137-5406156 MS.gene09712:intron 55.0%
GCCCAGCACTACCACTCACT+TGG + chr5.2:5406128-5406147 None:intergenic 60.0%
! GGTGGTGACCGATTTCGGTG+TGG - chr5.2:5405679-5405698 MS.gene09712:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 5405374 5406230 5405374 ID=MS.gene09712
chr5.2 mRNA 5405374 5406230 5405374 ID=MS.gene09712.t1;Parent=MS.gene09712
chr5.2 exon 5406163 5406230 5406163 ID=MS.gene09712.t1.exon1;Parent=MS.gene09712.t1
chr5.2 CDS 5406163 5406230 5406163 ID=cds.MS.gene09712.t1;Parent=MS.gene09712.t1
chr5.2 exon 5405658 5405967 5405658 ID=MS.gene09712.t1.exon2;Parent=MS.gene09712.t1
chr5.2 CDS 5405658 5405967 5405658 ID=cds.MS.gene09712.t1;Parent=MS.gene09712.t1
chr5.2 exon 5405374 5405571 5405374 ID=MS.gene09712.t1.exon3;Parent=MS.gene09712.t1
chr5.2 CDS 5405374 5405571 5405374 ID=cds.MS.gene09712.t1;Parent=MS.gene09712.t1
Gene Sequence

>MS.gene09712

ATGATGGCAATGTCGCAGGCGTTTCTTCGCTATGGAAGAATGGATCTGGTTGTGGTGCATGCTATCAAGTAATAACTTTACTTAACTTTACTATTATTTTACTCGTGTTAATTAATGAATATGAAAAAATATGATTTATCATATATTCATTAACTTATGTGACATATTTTGAAGTAATATATATTATAAAGGAAAAATTGTACCAACATCAACTAACAATTAGTTACTTATGAAATATAATAAATAATGATGAAATAGGTGAGGTGCAAAATACCAGAATTGTGTGACGAGAATGGAGCATATGTGGTGGTGACCGATTTCGGTGTGGGAGATAGGACAGACTTCATCATGAGTCCCCGTGGCTATTCAAATTTGGGCAAAAATGGTGATGCATCTGCAGAACTTTTTAAATATGGTGTGGTGGATATAGAATATAAAAGAATCCCTTGCAAATACAATGGCTATAATATCTTGTTTAAGGTCCATGAACGCAGCAAAAATCCTCACTACTTAGCCATTCTTATTCTCTATGTTGGTGGAACAAATGATGTAACTGCTGTTCAGTTGTGGCAGGTATACCTTTATTTTGAGATTGTTAATATTTTTTAGTTACATTTCTTGAAATTGAATTGGGTTTACTTGAAATGGTAATGGAACAGGAAGATTGCCAAGAATGGAGGCCAATGCGTAGGGCATTTGGAACAGTGTTTGATGCAGAGAACCCTCCAAGAGGTGATATCAAGTTGAGGCTCCAAGTGAGTGGTAGTGCTGGGCTGTATTGGGTGGAGTCCAAGACTGTTATCTCTAGTGATTGGGAAGCTGGGGCTGTTTATGACTCACAAATTCAGTTTGATTAA

Protein sequence

>MS.gene09712.t1

MMAMSQAFLRYGRMDLVVVHAIKCKIPELCDENGAYVVVTDFGVGDRTDFIMSPRGYSNLGKNGDASAELFKYGVVDIEYKRIPCKYNGYNILFKVHERSKNPHYLAILILYVGGTNDVTAVQLWQEDCQEWRPMRRAFGTVFDAENPPRGDIKLRLQVSGSAGLYWVESKTVISSDWEAGAVYDSQIQFD