AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21522

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21522.t1 MTR_5g013440 96.414 251 9 0 1 251 2 252 0.0 505
MS.gene21522.t1 MTR_4g099370 75.299 251 62 0 1 251 1 251 1.55e-149 417
MS.gene21522.t1 MTR_4g099400 60.355 169 67 0 81 249 10 178 4.81e-77 231
MS.gene21522.t1 MTR_4g098930 41.036 251 140 2 9 251 3 253 1.63e-64 202
MS.gene21522.t1 MTR_4g075790 41.176 221 126 3 31 250 26 243 2.25e-58 186
MS.gene21522.t1 MTR_4g075790 39.412 170 102 1 81 250 9 177 1.77e-43 146
MS.gene21522.t1 MTR_0809s0010 32.819 259 153 7 9 250 16 270 4.47e-34 124
MS.gene21522.t1 MTR_4g028640 31.739 230 147 7 26 250 40 264 2.40e-30 114
MS.gene21522.t1 MTR_0118s0070 34.802 227 135 8 33 250 53 275 3.67e-30 114
MS.gene21522.t1 MTR_2g090265 31.278 227 141 7 33 251 38 257 4.53e-30 113
MS.gene21522.t1 MTR_2g090270 30.531 226 142 7 33 250 40 258 5.74e-29 110
MS.gene21522.t1 MTR_0122s0020 30.973 226 141 7 33 250 46 264 1.53e-27 106
MS.gene21522.t1 MTR_4g102450 26.538 260 164 9 11 251 11 262 2.49e-23 95.5
MS.gene21522.t1 MTR_1g085460 29.185 233 144 9 31 250 33 257 2.56e-23 95.5
MS.gene21522.t1 MTR_7g111010 27.132 258 162 11 11 250 5 254 8.58e-23 94.0
MS.gene21522.t1 MTR_3g435430 28.926 242 156 9 17 250 14 247 1.03e-22 93.6
MS.gene21522.t1 MTR_7g013140 28.163 245 161 10 14 250 14 251 1.26e-21 90.9
MS.gene21522.t1 MTR_3g094040 28.829 222 143 8 31 244 34 248 1.40e-21 90.5
MS.gene21522.t1 MTR_3g094060 28.444 225 146 8 31 247 34 251 1.83e-21 90.5
MS.gene21522.t1 MTR_5g011210 26.695 236 146 6 31 251 40 263 1.87e-21 90.5
MS.gene21522.t1 MTR_7g110990 27.468 233 148 9 31 250 15 239 2.66e-21 89.7
MS.gene21522.t1 MTR_7g013120 27.642 246 163 8 14 250 14 253 8.16e-21 88.6
MS.gene21522.t1 MTR_8g058990 28.175 252 165 10 8 250 9 253 1.12e-20 88.2
MS.gene21522.t1 MTR_7g011060 29.565 230 148 10 25 251 23 241 2.28e-20 87.0
MS.gene21522.t1 MTR_5g075320 28.829 222 143 8 32 245 33 247 9.84e-20 85.5
MS.gene21522.t1 MTR_7g013150 28.185 259 169 10 1 250 3 253 1.12e-19 85.5
MS.gene21522.t1 MTR_3g466760 25.703 249 161 9 14 245 15 256 1.62e-19 85.1
MS.gene21522.t1 MTR_3g466790 25.397 252 164 9 11 245 6 250 1.82e-19 85.1
MS.gene21522.t1 MTR_5g041700 27.572 243 158 9 14 245 8 243 1.36e-18 82.4
MS.gene21522.t1 MTR_0122s0020 32.704 159 96 5 33 184 46 200 1.44e-18 81.6
MS.gene21522.t1 MTR_4g081950 27.273 242 161 8 11 244 44 278 1.45e-18 83.2
MS.gene21522.t1 MTR_8g464350 26.695 236 142 10 31 250 34 254 3.60e-18 81.6
MS.gene21522.t1 MTR_7g061720 25.214 234 158 8 24 247 28 254 4.13e-17 78.6
MS.gene21522.t1 MTR_4g099380 41.667 108 48 3 112 218 1 94 1.32e-16 77.8
MS.gene21522.t1 MTR_1g026020 23.810 231 158 9 31 250 32 255 3.66e-16 75.9
MS.gene21522.t1 MTR_0040s0140 28.112 249 160 10 11 247 13 254 5.84e-16 75.5
MS.gene21522.t1 MTR_5g079950 24.786 234 159 9 24 247 29 255 8.46e-16 75.1
MS.gene21522.t1 MTR_2g097030 25.439 228 151 9 31 247 37 256 2.93e-15 73.6
MS.gene21522.t1 MTR_2g090255 27.600 250 161 10 11 247 8 250 4.30e-14 70.1
MS.gene21522.t1 MTR_5g076950 27.660 235 143 11 31 251 44 265 5.99e-14 70.1
MS.gene21522.t1 MTR_6g012520 26.613 248 164 10 8 247 3 240 6.74e-13 66.6
MS.gene21522.t1 MTR_4g063975 26.667 180 117 6 31 200 35 209 1.05e-12 66.2
MS.gene21522.t1 MTR_3g064510 26.122 245 151 11 23 247 48 282 1.81e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21522.t1 AT4G17030 63.180 239 85 3 11 248 11 247 1.02e-113 327
MS.gene21522.t1 AT3G45970 39.614 207 122 3 47 250 15 221 9.18e-51 166
MS.gene21522.t1 AT4G38400 40.099 202 119 2 51 250 47 248 1.35e-48 161
MS.gene21522.t1 AT3G45970 38.916 203 121 3 51 250 46 248 7.90e-48 159
MS.gene21522.t1 AT3G45960 38.298 188 115 1 64 250 12 199 2.58e-47 156
MS.gene21522.t1 AT3G45960 37.129 202 125 2 51 250 46 247 3.72e-47 157
MS.gene21522.t1 AT1G65680 32.917 240 141 8 23 250 42 273 1.36e-34 125
MS.gene21522.t1 AT4G28250 30.534 262 163 9 5 251 4 261 4.82e-30 113
MS.gene21522.t1 AT2G20750 29.771 262 158 9 12 251 11 268 6.11e-28 108
MS.gene21522.t1 AT4G28250 29.389 262 160 10 5 251 4 255 5.33e-25 100
MS.gene21522.t1 AT2G40610 29.839 248 160 8 5 244 7 248 1.93e-24 98.2
MS.gene21522.t1 AT5G05290 29.730 222 142 7 32 245 36 251 2.98e-24 97.8
MS.gene21522.t1 AT5G39290 30.769 234 133 11 31 250 61 279 3.91e-23 95.5
MS.gene21522.t1 AT3G60570 29.200 250 152 10 14 250 20 257 1.11e-22 94.0
MS.gene21522.t1 AT5G39270 30.769 234 133 11 31 250 61 279 4.47e-22 92.4
MS.gene21522.t1 AT5G39310 30.531 226 143 9 32 250 94 312 6.20e-22 92.8
MS.gene21522.t1 AT4G01630 24.046 262 180 8 1 250 1 255 1.08e-21 90.9
MS.gene21522.t1 AT2G45110 31.579 228 138 8 34 250 39 259 2.03e-21 90.5
MS.gene21522.t1 AT5G56320 27.419 248 161 9 12 247 9 249 1.53e-20 87.8
MS.gene21522.t1 AT5G56320 27.419 248 161 9 12 247 9 249 1.53e-20 87.8
MS.gene21522.t1 AT3G60570 30.876 217 125 10 47 250 5 209 4.22e-20 85.9
MS.gene21522.t1 AT5G56320 27.419 248 161 9 12 247 9 249 4.88e-20 87.4
MS.gene21522.t1 AT5G39280 30.088 226 145 9 31 250 57 275 5.19e-20 87.0
MS.gene21522.t1 AT5G39300 30.088 226 145 9 31 250 58 276 8.42e-20 86.3
MS.gene21522.t1 AT2G03090 26.721 247 166 8 9 247 11 250 9.93e-20 85.9
MS.gene21522.t1 AT1G65681 27.962 211 140 6 47 250 17 222 1.65e-19 84.3
MS.gene21522.t1 AT2G20750 31.122 196 112 7 12 186 11 204 1.65e-18 81.6
MS.gene21522.t1 AT3G15370 27.734 256 150 11 11 247 8 247 5.43e-18 80.9
MS.gene21522.t1 AT5G02260 27.391 230 150 9 28 247 32 254 9.79e-18 80.5
MS.gene21522.t1 AT1G12560 24.123 228 159 6 31 250 40 261 4.72e-17 78.6
MS.gene21522.t1 AT2G39700 25.446 224 150 8 31 244 34 250 8.64e-17 77.8
MS.gene21522.t1 AT4G28250 30.457 197 115 7 5 187 4 192 1.76e-16 76.3
MS.gene21522.t1 AT4G28250 30.612 196 114 7 5 186 4 191 3.70e-16 75.1
MS.gene21522.t1 AT1G62980 23.228 254 175 7 12 251 10 257 6.92e-16 75.5
MS.gene21522.t1 AT3G29030 28.311 219 147 8 28 244 38 248 1.17e-15 74.7
MS.gene21522.t1 AT2G37640 26.432 227 150 8 31 247 39 258 1.55e-15 74.3
MS.gene21522.t1 AT5G39260 28.444 225 146 10 31 250 48 262 6.38e-15 72.8
MS.gene21522.t1 AT1G20190 25.431 232 151 10 32 250 30 252 4.12e-14 70.5
MS.gene21522.t1 AT4G38210 27.600 250 160 12 12 247 10 252 1.49e-12 65.9
MS.gene21522.t1 AT2G37640 26.066 211 139 8 47 247 30 233 1.59e-12 65.5
MS.gene21522.t1 AT3G03220 26.957 230 151 9 31 251 43 264 1.74e-12 65.9
MS.gene21522.t1 AT3G15370 25.926 216 131 9 47 247 76 277 1.76e-12 66.2

Find 67 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATTTGGTGCTTAAAATT+AGG 0.152890 5.1:-6364906 None:intergenic
TCCCCGTGGCTATTCAAATT+TGG 0.158342 5.1:+6365809 MS.gene21522:CDS
TGGCTATAATATCTTGTTTA+AGG 0.174105 5.1:+6365914 MS.gene21522:CDS
TGCTCTAGAGCAACCTATTA+TGG 0.246885 5.1:+6364987 MS.gene21522:CDS
CTTGGATTTGCATAACAATC+TGG 0.272291 5.1:-6365014 None:intergenic
ACAATCTGGGCTACCATAAT+AGG 0.288543 5.1:-6365000 None:intergenic
GCTTGTGGCTATGGTGATTA+TGG 0.309081 5.1:+6365422 MS.gene21522:CDS
TACGCATTGGCCTCCATTCT+TGG 0.310288 5.1:-6366124 None:intergenic
TGACACAAACAAAACCAATT+TGG 0.321541 5.1:-6364920 None:intergenic
GTGGTAGTGCTGGGCTGTAT+TGG 0.321854 5.1:+6366216 MS.gene21522:CDS
CCATTCTCGTCACACAATTC+TGG 0.326522 5.1:-6365729 None:intergenic
TGGTAGTGCTGGGCTGTATT+GGG 0.329472 5.1:+6366217 MS.gene21522:CDS
CCCCGTGGCTATTCAAATTT+GGG 0.333249 5.1:+6365810 MS.gene21522:CDS
ATTCCTAGTGATTGGGAAGC+TGG 0.347548 5.1:+6366257 MS.gene21522:CDS
AGAATGTTATTCCTAGTGAT+TGG 0.349568 5.1:+6366249 MS.gene21522:CDS
AGGCCAATGCGTAGGGCATT+TGG 0.356145 5.1:+6366134 MS.gene21522:CDS
ATCACTAGGAATAACATTCT+TGG 0.372273 5.1:-6366246 None:intergenic
CCGATTTCGGTGTGGGAGAT+AGG 0.376563 5.1:+6365769 MS.gene21522:CDS
CCTATCTCCCACACCGAAAT+CGG 0.384864 5.1:-6365769 None:intergenic
GCCATTCTTATTCTCTATGT+TGG 0.402224 5.1:+6365969 MS.gene21522:CDS
CTCCAAGTGAGTGGTAGTGC+TGG 0.409157 5.1:+6366206 MS.gene21522:CDS
TTCCTAGTGATTGGGAAGCT+GGG 0.431541 5.1:+6366258 MS.gene21522:CDS
TTAGAGGGAGCTTGTGGCTA+TGG 0.433052 5.1:+6365413 MS.gene21522:intron
AACTTGATCTCACCTCTTGG+AGG 0.448456 5.1:-6366179 None:intergenic
GAATGTTATTCCTAGTGATT+GGG 0.462804 5.1:+6366250 MS.gene21522:CDS
AACAGGAAGATTGCCAAGAA+TGG 0.466268 5.1:+6366111 MS.gene21522:intron
ATTCTTATTCTCTATGTTGG+TGG 0.472042 5.1:+6365972 MS.gene21522:CDS
TATGTGGTGGTGACCGATTT+CGG 0.479504 5.1:+6365756 MS.gene21522:CDS
TCGCAGGCGTTTCTTCGCTA+TGG 0.490569 5.1:+6365468 MS.gene21522:CDS
TCGCTATGGAAGAATGGATC+TGG 0.490619 5.1:+6365482 MS.gene21522:CDS
CTCAACTTGATCTCACCTCT+TGG 0.495730 5.1:-6366182 None:intergenic
TTGGATTTGCATAACAATCT+GGG 0.496289 5.1:-6365013 None:intergenic
CCAGAATTGTGTGACGAGAA+TGG 0.501363 5.1:+6365729 MS.gene21522:CDS
TCCTAGTGATTGGGAAGCTG+GGG 0.513323 5.1:+6366259 MS.gene21522:CDS
TTATAGCCATTGTATTTGCA+AGG 0.532628 5.1:-6365900 None:intergenic
GCCCAGCACTACCACTCACT+TGG 0.534848 5.1:-6366208 None:intergenic
ACTTGATCTCACCTCTTGGA+GGG 0.535508 5.1:-6366178 None:intergenic
ACCAACATAGAGAATAAGAA+TGG 0.537864 5.1:-6365970 None:intergenic
GCCCAAATTTGAATAGCCAC+GGG 0.544992 5.1:-6365811 None:intergenic
TGCCCAAATTTGAATAGCCA+CGG 0.546821 5.1:-6365812 None:intergenic
GTTCCAAATGCCCTACGCAT+TGG 0.550110 5.1:-6366137 None:intergenic
GTAAATGATGGCAATGTCGC+AGG 0.552094 5.1:+6365452 MS.gene21522:CDS
TCCAAGTGAGTGGTAGTGCT+GGG 0.552324 5.1:+6366207 MS.gene21522:CDS
GTTTCTTCGCTATGGAAGAA+TGG 0.552967 5.1:+6365476 MS.gene21522:CDS
AAGAATGGAGGCCAATGCGT+AGG 0.554025 5.1:+6366126 MS.gene21522:CDS
TGGAAGAATGGATCTGGTTG+TGG 0.555017 5.1:+6365488 MS.gene21522:CDS
TCTTGAGAGGAACACAAGAC+AGG 0.557963 5.1:-6364954 None:intergenic
TATAGCCATTGTATTTGCAA+GGG 0.561411 5.1:-6365899 None:intergenic
GTGGTGACCGATTTCGGTGT+GGG 0.566642 5.1:+6365762 MS.gene21522:CDS
AACTGCTGTTCAGTTGTGGC+AGG 0.569473 5.1:+6366007 MS.gene21522:CDS
ATAAGAATGGCTAAGTAGTG+AGG 0.575706 5.1:-6365957 None:intergenic
AGAATGGAGGCCAATGCGTA+GGG 0.588746 5.1:+6366127 MS.gene21522:CDS
TATGGACAGACAGTAAATGA+TGG 0.603311 5.1:+6365440 MS.gene21522:CDS
TAGTGCTGGGCTGTATTGGG+TGG 0.610771 5.1:+6366220 MS.gene21522:CDS
AGGAAGATTGCCAAGAATGG+AGG 0.614849 5.1:+6366114 MS.gene21522:intron
TGACGAGAATGGAGCATATG+TGG 0.619740 5.1:+6365740 MS.gene21522:CDS
AGAATCCCTTGCAAATACAA+TGG 0.620915 5.1:+6365894 MS.gene21522:CDS
GCAAACAAAAGAGTCTTGAG+AGG 0.624968 5.1:-6364967 None:intergenic
GCCCCAGCTTCCCAATCACT+AGG 0.636884 5.1:-6366260 None:intergenic
ATGTAACTGCTGTTCAGTTG+TGG 0.651186 5.1:+6366003 MS.gene21522:CDS
GGTGGTGACCGATTTCGGTG+TGG 0.653687 5.1:+6365761 MS.gene21522:CDS
AAGTTGAGGCTCCAAGTGAG+TGG 0.655665 5.1:+6366197 MS.gene21522:CDS
GACTTCATCATGAGTCCCCG+TGG 0.688519 5.1:+6365795 MS.gene21522:CDS
GATGCAGAGAACCCTCCAAG+AGG 0.690257 5.1:+6366167 MS.gene21522:CDS
CCCAAATTTGAATAGCCACG+GGG 0.693150 5.1:-6365810 None:intergenic
CAAGAGGTGAGATCAAGTTG+AGG 0.697595 5.1:+6366183 MS.gene21522:CDS
CGAGAATGGAGCATATGTGG+TGG 0.757236 5.1:+6365743 MS.gene21522:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAAATAATGATGAAAT+AGG + chr5.1:6365692-6365711 MS.gene21522:intron 10.0%
!! TGAAGTAATATATATTATAA+AGG + chr5.1:6365625-6365644 MS.gene21522:intron 10.0%
!! TATTAACAATCTCAAAATAA+AGG - chr5.1:6366037-6366056 None:intergenic 15.0%
!!! ATTCAATTTTTATCTCAAAT+TGG + chr5.1:6365191-6365210 MS.gene21522:intron 15.0%
!! ACACATTTATAAAAATTGAC+AGG - chr5.1:6365231-6365250 None:intergenic 20.0%
!! ACATTTCTTGAAATTGAATT+GGG + chr5.1:6366068-6366087 MS.gene21522:intron 20.0%
!! CACATTTATAAAAATTGACA+GGG - chr5.1:6365230-6365249 None:intergenic 20.0%
!! TACATTTCTTGAAATTGAAT+TGG + chr5.1:6366067-6366086 MS.gene21522:intron 20.0%
! AAATAATGATGAAATAGGTG+AGG + chr5.1:6365697-6365716 MS.gene21522:intron 25.0%
! CATAAAATACATAATCTGAG+TGG + chr5.1:6365377-6365396 MS.gene21522:intron 25.0%
! TATTCAAATTTGGGCAAAAA+TGG + chr5.1:6365819-6365838 MS.gene21522:CDS 25.0%
!! AACTAATTGTTAGTTGATGT+TGG - chr5.1:6365661-6365680 None:intergenic 25.0%
!! AGAACTTTTTAAATATGGTG+TGG + chr5.1:6365854-6365873 MS.gene21522:CDS 25.0%
!! CATTGAATCGGTTTATAAAT+CGG - chr5.1:6365298-6365317 None:intergenic 25.0%
!! TCTGCAGAACTTTTTAAATA+TGG + chr5.1:6365849-6365868 MS.gene21522:CDS 25.0%
!! TGGCTATAATATCTTGTTTA+AGG + chr5.1:6365914-6365933 MS.gene21522:CDS 25.0%
!!! CAGTATACAACATTTTTTTC+CGG + chr5.1:6365321-6365340 MS.gene21522:intron 25.0%
ACCAACATAGAGAATAAGAA+TGG - chr5.1:6365973-6365992 None:intergenic 30.0%
AGAATGTTATTCCTAGTGAT+TGG + chr5.1:6366249-6366268 MS.gene21522:CDS 30.0%
ATCACTAGGAATAACATTCT+TGG - chr5.1:6366249-6366268 None:intergenic 30.0%
ATTAGTAGAAGACACTTACT+TGG - chr5.1:6365035-6365054 None:intergenic 30.0%
ATTCTTATTCTCTATGTTGG+TGG + chr5.1:6365972-6365991 MS.gene21522:CDS 30.0%
GAATGTTATTCCTAGTGATT+GGG + chr5.1:6366250-6366269 MS.gene21522:CDS 30.0%
TATAGCCATTGTATTTGCAA+GGG - chr5.1:6365902-6365921 None:intergenic 30.0%
TGAATTGGGTTTACTTGAAA+TGG + chr5.1:6366082-6366101 MS.gene21522:intron 30.0%
TGACACAAACAAAACCAATT+TGG - chr5.1:6364923-6364942 None:intergenic 30.0%
TGTATACTGTCTCATTGAAT+CGG - chr5.1:6365310-6365329 None:intergenic 30.0%
TTATAGCCATTGTATTTGCA+AGG - chr5.1:6365903-6365922 None:intergenic 30.0%
TTGGATTTGCATAACAATCT+GGG - chr5.1:6365016-6365035 None:intergenic 30.0%
! ACTTTTTAAATATGGTGTGG+TGG + chr5.1:6365857-6365876 MS.gene21522:CDS 30.0%
! CCTAATTTTAAGCACCAAAT+TGG + chr5.1:6364906-6364925 MS.gene21522:CDS 30.0%
!! CCAATTTGGTGCTTAAAATT+AGG - chr5.1:6364909-6364928 None:intergenic 30.0%
AAAAATTGACAGGGTTGAGT+AGG - chr5.1:6365221-6365240 None:intergenic 35.0%
ACCTAGACATTGCATTACTT+TGG + chr5.1:6365347-6365366 MS.gene21522:intron 35.0%
AGAATCCCTTGCAAATACAA+TGG + chr5.1:6365894-6365913 MS.gene21522:CDS 35.0%
ATAAGAATGGCTAAGTAGTG+AGG - chr5.1:6365960-6365979 None:intergenic 35.0%
CTTGGATTTGCATAACAATC+TGG - chr5.1:6365017-6365036 None:intergenic 35.0%
GCCATTCTTATTCTCTATGT+TGG + chr5.1:6365969-6365988 MS.gene21522:CDS 35.0%
GGGTTTACTTGAAATGGTAA+TGG + chr5.1:6366088-6366107 MS.gene21522:intron 35.0%
TATGGACAGACAGTAAATGA+TGG + chr5.1:6365440-6365459 MS.gene21522:CDS 35.0%
!! ACTTGAAATGGTAATGGAAC+AGG + chr5.1:6366094-6366113 MS.gene21522:intron 35.0%
!! GGCTATTAACCTGTTTTAGA+GGG + chr5.1:6365398-6365417 MS.gene21522:intron 35.0%
!! TGGCTATTAACCTGTTTTAG+AGG + chr5.1:6365397-6365416 MS.gene21522:intron 35.0%
!!! GAGTGTTGTGCACATTTTTT+AGG + chr5.1:6365254-6365273 MS.gene21522:intron 35.0%
AACAGGAAGATTGCCAAGAA+TGG + chr5.1:6366111-6366130 MS.gene21522:intron 40.0%
AATGCAATGTCTAGGTTCAC+CGG - chr5.1:6365343-6365362 None:intergenic 40.0%
ACAATCTGGGCTACCATAAT+AGG - chr5.1:6365003-6365022 None:intergenic 40.0%
ATGTAACTGCTGTTCAGTTG+TGG + chr5.1:6366003-6366022 MS.gene21522:CDS 40.0%
GCAAACAAAAGAGTCTTGAG+AGG - chr5.1:6364970-6364989 None:intergenic 40.0%
GCCAAAGTAATGCAATGTCT+AGG - chr5.1:6365351-6365370 None:intergenic 40.0%
GTTTCTTCGCTATGGAAGAA+TGG + chr5.1:6365476-6365495 MS.gene21522:CDS 40.0%
TGCCCAAATTTGAATAGCCA+CGG - chr5.1:6365815-6365834 None:intergenic 40.0%
!! TGCTCTAGAGCAACCTATTA+TGG + chr5.1:6364987-6365006 MS.gene21522:CDS 40.0%
AACTTGATCTCACCTCTTGG+AGG - chr5.1:6366182-6366201 None:intergenic 45.0%
ACTTGATCTCACCTCTTGGA+GGG - chr5.1:6366181-6366200 None:intergenic 45.0%
AGGAAGATTGCCAAGAATGG+AGG + chr5.1:6366114-6366133 MS.gene21522:intron 45.0%
ATTCCTAGTGATTGGGAAGC+TGG + chr5.1:6366257-6366276 MS.gene21522:CDS 45.0%
CCAGAATTGTGTGACGAGAA+TGG + chr5.1:6365729-6365748 MS.gene21522:CDS 45.0%
CCATTCTCGTCACACAATTC+TGG - chr5.1:6365732-6365751 None:intergenic 45.0%
CCCAAATTTGAATAGCCACG+GGG - chr5.1:6365813-6365832 None:intergenic 45.0%
CCCCGTGGCTATTCAAATTT+GGG + chr5.1:6365810-6365829 MS.gene21522:CDS 45.0%
CTCAACTTGATCTCACCTCT+TGG - chr5.1:6366185-6366204 None:intergenic 45.0%
GCCCAAATTTGAATAGCCAC+GGG - chr5.1:6365814-6365833 None:intergenic 45.0%
GCTTGTGGCTATGGTGATTA+TGG + chr5.1:6365422-6365441 MS.gene21522:CDS 45.0%
GTAAATGATGGCAATGTCGC+AGG + chr5.1:6365452-6365471 MS.gene21522:CDS 45.0%
TCCCCGTGGCTATTCAAATT+TGG + chr5.1:6365809-6365828 MS.gene21522:CDS 45.0%
TCGCTATGGAAGAATGGATC+TGG + chr5.1:6365482-6365501 MS.gene21522:CDS 45.0%
TCTTGAGAGGAACACAAGAC+AGG - chr5.1:6364957-6364976 None:intergenic 45.0%
TGACGAGAATGGAGCATATG+TGG + chr5.1:6365740-6365759 MS.gene21522:CDS 45.0%
TGGAAGAATGGATCTGGTTG+TGG + chr5.1:6365488-6365507 MS.gene21522:CDS 45.0%
TTCCTAGTGATTGGGAAGCT+GGG + chr5.1:6366258-6366277 MS.gene21522:CDS 45.0%
! CAAGAGGTGAGATCAAGTTG+AGG + chr5.1:6366183-6366202 MS.gene21522:CDS 45.0%
! GAGGATTTTTGCTGCGTTCA+TGG - chr5.1:6365941-6365960 None:intergenic 45.0%
! TATGTGGTGGTGACCGATTT+CGG + chr5.1:6365756-6365775 MS.gene21522:CDS 45.0%
AACTGCTGTTCAGTTGTGGC+AGG + chr5.1:6366007-6366026 MS.gene21522:CDS 50.0%
AAGAATGGAGGCCAATGCGT+AGG + chr5.1:6366126-6366145 MS.gene21522:CDS 50.0%
AAGTTGAGGCTCCAAGTGAG+TGG + chr5.1:6366197-6366216 MS.gene21522:CDS 50.0%
AGAATGGAGGCCAATGCGTA+GGG + chr5.1:6366127-6366146 MS.gene21522:CDS 50.0%
CCTATCTCCCACACCGAAAT+CGG - chr5.1:6365772-6365791 None:intergenic 50.0%
CGAGAATGGAGCATATGTGG+TGG + chr5.1:6365743-6365762 MS.gene21522:CDS 50.0%
GTTCCAAATGCCCTACGCAT+TGG - chr5.1:6366140-6366159 None:intergenic 50.0%
TCCTAGTGATTGGGAAGCTG+GGG + chr5.1:6366259-6366278 MS.gene21522:CDS 50.0%
TTAGAGGGAGCTTGTGGCTA+TGG + chr5.1:6365413-6365432 MS.gene21522:intron 50.0%
! TACGCATTGGCCTCCATTCT+TGG - chr5.1:6366127-6366146 None:intergenic 50.0%
! TCCAAGTGAGTGGTAGTGCT+GGG + chr5.1:6366207-6366226 MS.gene21522:CDS 50.0%
! TGGTAGTGCTGGGCTGTATT+GGG + chr5.1:6366217-6366236 MS.gene21522:CDS 50.0%
!! CCACAAGCTCCCTCTAAAAC+AGG - chr5.1:6365410-6365429 None:intergenic 50.0%
!!! CCTGTTTTAGAGGGAGCTTG+TGG + chr5.1:6365407-6365426 MS.gene21522:intron 50.0%
GACTTCATCATGAGTCCCCG+TGG + chr5.1:6365795-6365814 MS.gene21522:CDS 55.0%
GATGCAGAGAACCCTCCAAG+AGG + chr5.1:6366167-6366186 MS.gene21522:CDS 55.0%
TCGCAGGCGTTTCTTCGCTA+TGG + chr5.1:6365468-6365487 MS.gene21522:CDS 55.0%
! AGGCCAATGCGTAGGGCATT+TGG + chr5.1:6366134-6366153 MS.gene21522:CDS 55.0%
! CCGATTTCGGTGTGGGAGAT+AGG + chr5.1:6365769-6365788 MS.gene21522:CDS 55.0%
! CTCCAAGTGAGTGGTAGTGC+TGG + chr5.1:6366206-6366225 MS.gene21522:CDS 55.0%
! GTGGTAGTGCTGGGCTGTAT+TGG + chr5.1:6366216-6366235 MS.gene21522:CDS 55.0%
! GTGGTGACCGATTTCGGTGT+GGG + chr5.1:6365762-6365781 MS.gene21522:CDS 55.0%
! TAGTGCTGGGCTGTATTGGG+TGG + chr5.1:6366220-6366239 MS.gene21522:CDS 55.0%
GCCCAGCACTACCACTCACT+TGG - chr5.1:6366211-6366230 None:intergenic 60.0%
GCCCCAGCTTCCCAATCACT+AGG - chr5.1:6366263-6366282 None:intergenic 60.0%
! GGTGGTGACCGATTTCGGTG+TGG + chr5.1:6365761-6365780 MS.gene21522:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 6364900 6366313 6364900 ID=MS.gene21522
chr5.1 mRNA 6364900 6366313 6364900 ID=MS.gene21522.t1;Parent=MS.gene21522
chr5.1 exon 6364900 6365035 6364900 ID=MS.gene21522.t1.exon1;Parent=MS.gene21522.t1
chr5.1 CDS 6364900 6365035 6364900 ID=cds.MS.gene21522.t1;Parent=MS.gene21522.t1
chr5.1 exon 6365417 6365523 6365417 ID=MS.gene21522.t1.exon2;Parent=MS.gene21522.t1
chr5.1 CDS 6365417 6365523 6365417 ID=cds.MS.gene21522.t1;Parent=MS.gene21522.t1
chr5.1 exon 6365714 6366028 6365714 ID=MS.gene21522.t1.exon3;Parent=MS.gene21522.t1
chr5.1 CDS 6365714 6366028 6365714 ID=cds.MS.gene21522.t1;Parent=MS.gene21522.t1
chr5.1 exon 6366116 6366313 6366116 ID=MS.gene21522.t1.exon4;Parent=MS.gene21522.t1
chr5.1 CDS 6366116 6366313 6366116 ID=cds.MS.gene21522.t1;Parent=MS.gene21522.t1
Gene Sequence

>MS.gene21522

ATGGAACCTAATTTTAAGCACCAAATTGGTTTTGTTTGTGTCATATTGCTCTTACCTGTCTTGTGTTCCTCTCAAGACTCTTTTGTTTGCTCTAGAGCAACCTATTATGGTAGCCCAGATTGTTATGCAAATCCAAGTAAGTGTCTTCTACTAATATATACTCGTGCAAAATTTATTATATATAGTGCATGTGACATAGCACATTATTTAAAAAAAATTATACATAAAATTTTAATATATTAATTATTATGTTACTATTTTCTTTGTATTTTAATCTCGATCATTTATCTCATTCAATTTTTATCTCAAATTGGTTAACCTACTCAACCCTGTCAATTTTTATAAATGTGTATAGAGTGTTGTGCACATTTTTTAGGTTTGACATGTTTTTGCGACCGATTTATAAACCGATTCAATGAGACAGTATACAACATTTTTTTCCGGTGAACCTAGACATTGCATTACTTTGGCTCTTTGCATAAAATACATAATCTGAGTGGCTATTAACCTGTTTTAGAGGGAGCTTGTGGCTATGGTGATTATGGACAGACAGTAAATGATGGCAATGTCGCAGGCGTTTCTTCGCTATGGAAGAATGGATCTGGTTGTGGTGCATGCTATCAAGTAATAACTTTACTTAACTTTACTATTATTTTACGCGTGTTAATTAATGAATATGAAAAAATATGATTTATCATATATTCATTAACTGATGTGACATATTTTGAAGTAATATATATTATAAAGGAAAAATTGTACCAACATCAACTAACAATTAGTTACTTATGAAATATAATAAATAATGATGAAATAGGTGAGGTGCAAAATACCAGAATTGTGTGACGAGAATGGAGCATATGTGGTGGTGACCGATTTCGGTGTGGGAGATAGGACAGACTTCATCATGAGTCCCCGTGGCTATTCAAATTTGGGCAAAAATGGTGATGCATCTGCAGAACTTTTTAAATATGGTGTGGTGGATATAGAATATAAAAGAATCCCTTGCAAATACAATGGCTATAATATCTTGTTTAAGGTCCATGAACGCAGCAAAAATCCTCACTACTTAGCCATTCTTATTCTCTATGTTGGTGGAACAAATGATGTAACTGCTGTTCAGTTGTGGCAGGTATACCTTTATTTTGAGATTGTTAATATTTTTTTAGTTACATTTCTTGAAATTGAATTGGGTTTACTTGAAATGGTAATGGAACAGGAAGATTGCCAAGAATGGAGGCCAATGCGTAGGGCATTTGGAACAGTGTTTGATGCAGAGAACCCTCCAAGAGGTGAGATCAAGTTGAGGCTCCAAGTGAGTGGTAGTGCTGGGCTGTATTGGGTGGAGTCCAAGAATGTTATTCCTAGTGATTGGGAAGCTGGGGCTGTTTATGACTCACAAATTCAGTTTGATTAA
Protein sequence

>MS.gene21522.t1

MEPNFKHQIGFVCVILLLPVLCSSQDSFVCSRATYYGSPDCYANPKGACGYGDYGQTVNDGNVAGVSSLWKNGSGCGACYQVRCKIPELCDENGAYVVVTDFGVGDRTDFIMSPRGYSNLGKNGDASAELFKYGVVDIEYKRIPCKYNGYNILFKVHERSKNPHYLAILILYVGGTNDVTAVQLWQEDCQEWRPMRRAFGTVFDAENPPRGEIKLRLQVSGSAGLYWVESKNVIPSDWEAGAVYDSQIQFD