AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38598


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38598.t1 MTR_3g102980 93.939 198 7 3 1 195 41 236 5.15e-118 334
MS.gene38598.t1 MTR_1g018420 57.500 200 67 6 6 193 37 230 4.21e-68 208
MS.gene38598.t1 MTR_1g106730 53.171 205 55 11 7 187 55 242 4.79e-52 167
MS.gene38598.t1 MTR_0160s0030 40.152 132 53 4 44 175 240 345 2.00e-19 84.7
MS.gene38598.t1 MTR_4g027440 45.652 92 48 2 36 125 212 303 4.07e-19 84.0
MS.gene38598.t1 MTR_1g067650 30.769 169 90 3 18 169 1 159 8.85e-19 79.7
MS.gene38598.t1 MTR_1g067640 30.357 168 90 3 19 169 1 158 5.37e-18 77.8
MS.gene38598.t1 MTR_4g028610 34.211 152 64 3 45 160 228 379 1.01e-17 80.5
MS.gene38598.t1 MTR_7g023560 34.375 128 70 2 12 139 12 125 2.95e-17 76.3
MS.gene38598.t1 MTR_1g090703 30.698 215 94 9 2 163 91 303 4.48e-17 78.2
MS.gene38598.t1 MTR_1g062780 32.178 202 80 8 12 167 81 271 9.47e-17 76.6
MS.gene38598.t1 MTR_5g099040 35.915 142 66 3 8 124 94 235 2.30e-16 75.5
MS.gene38598.t1 MTR_1g067660 33.529 170 85 5 7 169 6 154 7.04e-16 72.0
MS.gene38598.t1 MTR_5g062300 40.625 128 65 3 8 124 81 208 1.94e-15 72.4
MS.gene38598.t1 MTR_7g100100 43.038 79 38 2 44 122 34 105 2.14e-15 70.9
MS.gene38598.t1 MTR_4g109340 38.514 148 49 8 45 160 349 486 1.06e-14 72.0
MS.gene38598.t1 MTR_1g115345 39.496 119 60 3 4 122 12 118 1.15e-14 69.7
MS.gene38598.t1 MTR_2g084315 29.907 214 96 6 1 160 60 273 2.27e-14 70.1
MS.gene38598.t1 MTR_2g096540 37.037 108 43 2 15 122 13 95 2.32e-14 67.0
MS.gene38598.t1 MTR_4g100860 31.638 177 73 6 4 160 153 301 3.90e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38598.t1 AT1G27730 52.660 188 74 5 7 191 50 225 1.11e-48 158
MS.gene38598.t1 AT5G04340 50.777 193 80 7 1 182 41 229 4.88e-47 154
MS.gene38598.t1 AT3G19580 51.282 195 78 7 7 187 66 257 8.46e-46 152
MS.gene38598.t1 AT3G19580 51.282 195 78 7 7 187 66 257 8.46e-46 152
MS.gene38598.t1 AT5G43170 50.543 184 51 8 7 186 45 192 7.22e-39 132
MS.gene38598.t1 AT3G49930 48.718 156 61 4 7 160 55 193 1.14e-33 119
MS.gene38598.t1 AT5G67450 42.541 181 75 5 7 160 59 237 7.01e-31 113
MS.gene38598.t1 AT2G45120 40.237 169 78 5 4 160 143 300 1.59e-24 98.2
MS.gene38598.t1 AT3G60580 37.209 172 79 4 4 160 119 276 2.98e-24 97.1
MS.gene38598.t1 AT2G28200 33.846 195 91 7 6 163 70 263 4.19e-23 94.0
MS.gene38598.t1 AT1G49900 51.579 95 40 2 45 138 194 283 5.62e-22 93.2
MS.gene38598.t1 AT1G49900 40.000 135 72 3 43 177 749 874 1.75e-18 83.2
MS.gene38598.t1 AT5G56200 47.423 97 38 2 44 127 342 438 1.01e-20 89.4
MS.gene38598.t1 AT5G03510 33.668 199 92 5 3 163 64 260 1.91e-19 84.0
MS.gene38598.t1 AT1G02040 33.103 145 71 3 5 123 104 248 3.95e-17 78.2
MS.gene38598.t1 AT1G02030 34.194 155 79 4 7 160 123 255 1.13e-16 76.3
MS.gene38598.t1 AT4G35280 38.400 125 53 4 8 124 138 246 1.44e-16 76.3
MS.gene38598.t1 AT3G46080 40.171 117 46 3 9 122 12 107 4.95e-16 72.4
MS.gene38598.t1 AT2G17180 31.481 162 72 6 8 160 121 252 7.17e-16 73.9
MS.gene38598.t1 AT2G37430 48.718 78 31 1 45 122 48 116 9.82e-16 72.0
MS.gene38598.t1 AT5G59820 37.815 119 52 3 15 133 18 114 1.21e-15 71.2
MS.gene38598.t1 AT3G46090 37.008 127 60 3 2 127 6 113 1.87e-15 70.9
MS.gene38598.t1 AT5G04390 28.509 228 93 7 8 166 96 322 2.87e-15 73.2
MS.gene38598.t1 AT3G53600 42.308 78 33 2 45 122 50 115 1.13e-14 68.9
MS.gene38598.t1 AT2G28710 39.640 111 43 3 12 122 14 100 1.87e-14 68.2
MS.gene38598.t1 AT3G10470 38.235 102 40 4 44 122 182 283 1.28e-12 65.9
MS.gene38598.t1 AT3G46070 34.545 110 55 3 13 122 16 108 1.49e-12 63.2
MS.gene38598.t1 AT5G61470 36.250 80 41 2 44 123 228 297 7.02e-11 60.5

Find 51 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCTACTGGACAGGCTTT+GGG 0.284852 3.2:-24606872 MS.gene38598:CDS
TTGCGGACATAACAGCTTTC+CGG 0.302034 3.2:+24606999 None:intergenic
CTTCCCTACTGGACAGGCTT+TGG 0.307779 3.2:-24606873 MS.gene38598:CDS
GGCAGCGTTGGTGCCGGTGC+CGG 0.399502 3.2:-24606824 MS.gene38598:CDS
GTTGGTGCCGGTGCCGGTGC+TGG 0.425482 3.2:-24606818 MS.gene38598:CDS
CATAACAGCTTTCCGGTGAC+TGG 0.439265 3.2:+24607006 None:intergenic
GGCTTCTTTGCAGCAGGTAA+AGG 0.446061 3.2:+24606668 None:intergenic
AATTTCCAGCTTGAAAAGAC+AGG 0.452679 3.2:+24606646 None:intergenic
CTGATCTTCAGCGGTGGTTG+CGG 0.460016 3.2:+24606982 None:intergenic
TTTGAAAAGTCCGGAAAAGC+CGG 0.466243 3.2:+24606722 None:intergenic
ACTGGCTTTGTGTCCACCTA+GGG 0.475209 3.2:+24607024 None:intergenic
CTTACCGTTGGAAGCAGAGC+TGG 0.488264 3.2:+24606931 None:intergenic
GCATTTGTGACTGAGTTTAA+CGG 0.491121 3.2:+24607081 None:intergenic
ATGAGTCTTGTTCTTACCGT+TGG 0.503655 3.2:+24606919 None:intergenic
TGTCACAAATCCTTCCCTAC+TGG 0.506283 3.2:-24606884 MS.gene38598:CDS
AGACAGGGCTTCTTTGCAGC+AGG 0.516606 3.2:+24606662 None:intergenic
TACGAAGGCAGCGTTGGTGC+CGG 0.519961 3.2:-24606830 MS.gene38598:CDS
TTGAAAAGTCCGGAAAAGCC+GGG 0.523321 3.2:+24606723 None:intergenic
GACTGGCTTTGTGTCCACCT+AGG 0.526559 3.2:+24607023 None:intergenic
CAAATCCTTCCCTACTGGAC+AGG 0.527321 3.2:-24606879 MS.gene38598:CDS
CTGAGTTTAACGGTGGTTAG+CGG 0.533899 3.2:+24607091 None:intergenic
CCTCCCAAAGCCTGTCCAGT+AGG 0.538116 3.2:+24606869 None:intergenic
AGGGAAGGATTTGTGACAGA+TGG 0.550231 3.2:+24606889 None:intergenic
TTCTCATCTTATCAAGCCCT+AGG 0.550654 3.2:-24607040 MS.gene38598:CDS
GTAACTGCCTCTGAAGGAGT+TGG 0.551176 3.2:-24606785 MS.gene38598:CDS
CTCCCAAAGCCTGTCCAGTA+GGG 0.562622 3.2:+24606870 None:intergenic
TGTCACTACGAAGGCAGCGT+TGG 0.571767 3.2:-24606836 MS.gene38598:CDS
GGTAGTCACGGCGGATGAAG+TGG 0.575582 3.2:+24606952 None:intergenic
GCTACTTCCAGCACCGGCAC+CGG 0.578492 3.2:+24606811 None:intergenic
TAAGATCAAAATCACGGTGG+TGG 0.583762 3.2:+24606750 None:intergenic
CAAAGCCTGTCCAGTAGGGA+AGG 0.583815 3.2:+24606874 None:intergenic
GGAAGCAGAGCTGGTAGTCA+CGG 0.585255 3.2:+24606940 None:intergenic
CTCATCATGCTTGCTCGCAG+CGG 0.585296 3.2:-24607151 MS.gene38598:CDS
GTGGACACAAAGCCAGTCAC+CGG 0.585980 3.2:-24607018 MS.gene38598:CDS
ATTTCCAGCTTGAAAAGACA+GGG 0.591447 3.2:+24606647 None:intergenic
TACAGCGCTACTTCCAGCAC+CGG 0.591699 3.2:+24606805 None:intergenic
ATTATTGATCAGTAATGAGA+AGG 0.592327 3.2:+24606623 None:intergenic
AGCGCTGTAACTGCCTCTGA+AGG 0.607763 3.2:-24606791 MS.gene38598:CDS
CCTACTGGACAGGCTTTGGG+AGG 0.612476 3.2:-24606869 MS.gene38598:CDS
TCATCTTATCAAGCCCTAGG+TGG 0.625860 3.2:-24607037 MS.gene38598:CDS
TGACGATCCAACTCCTTCAG+AGG 0.633382 3.2:+24606778 None:intergenic
ACTACCAGCTCTGCTTCCAA+CGG 0.645257 3.2:-24606935 MS.gene38598:CDS
GGAGGTTAAGATCAAAATCA+CGG 0.658091 3.2:+24606744 None:intergenic
AGTCACGGCGGATGAAGTGG+TGG 0.674100 3.2:+24606955 None:intergenic
CACAAGCGTTGTCACTACGA+AGG 0.677003 3.2:-24606845 MS.gene38598:CDS
TTTGTGACTGAGTTTAACGG+TGG 0.692042 3.2:+24607084 None:intergenic
AGCAGAGCTGGTAGTCACGG+CGG 0.700777 3.2:+24606943 None:intergenic
GGTGATCTGATCTTCAGCGG+TGG 0.700953 3.2:+24606976 None:intergenic
GGTGGTGATCTGATCTTCAG+CGG 0.704088 3.2:+24606973 None:intergenic
AAAAGTCCGGAAAAGCCGGG+AGG 0.720865 3.2:+24606726 None:intergenic
GGTTAAGATCAAAATCACGG+TGG 0.807668 3.2:+24606747 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACTTCTTTGAAAAGTC+CGG + chr3.2:24607117-24607136 None:intergenic 25.0%
! CTTTTCAAAGAAGTTTTTCG+TGG - chr3.2:24607119-24607138 MS.gene38598:CDS 30.0%
AATTTCCAGCTTGAAAAGAC+AGG + chr3.2:24607184-24607203 None:intergenic 35.0%
ATTTCCAGCTTGAAAAGACA+GGG + chr3.2:24607183-24607202 None:intergenic 35.0%
CAACGACAAAAAGTCTGATT+CGG - chr3.2:24606705-24606724 MS.gene38598:CDS 35.0%
GCATTTGTGACTGAGTTTAA+CGG + chr3.2:24606749-24606768 None:intergenic 35.0%
GGAGGTTAAGATCAAAATCA+CGG + chr3.2:24607086-24607105 None:intergenic 35.0%
!! TGATTTTGATCTTAACCTCC+CGG - chr3.2:24607086-24607105 MS.gene38598:CDS 35.0%
GGTTAAGATCAAAATCACGG+TGG + chr3.2:24607083-24607102 None:intergenic 40.0%
TAAGATCAAAATCACGGTGG+TGG + chr3.2:24607080-24607099 None:intergenic 40.0%
TTCTCATCTTATCAAGCCCT+AGG - chr3.2:24606787-24606806 MS.gene38598:CDS 40.0%
TTTGAAAAGTCCGGAAAAGC+CGG + chr3.2:24607108-24607127 None:intergenic 40.0%
TTTGTGACTGAGTTTAACGG+TGG + chr3.2:24606746-24606765 None:intergenic 40.0%
!! ATGAGTCTTGTTCTTACCGT+TGG + chr3.2:24606911-24606930 None:intergenic 40.0%
AGGGAAGGATTTGTGACAGA+TGG + chr3.2:24606941-24606960 None:intergenic 45.0%
CTGAGTTTAACGGTGGTTAG+CGG + chr3.2:24606739-24606758 None:intergenic 45.0%
TCATCTTATCAAGCCCTAGG+TGG - chr3.2:24606790-24606809 MS.gene38598:CDS 45.0%
TGTCACAAATCCTTCCCTAC+TGG - chr3.2:24606943-24606962 MS.gene38598:CDS 45.0%
TTGAAAAGTCCGGAAAAGCC+GGG + chr3.2:24607107-24607126 None:intergenic 45.0%
TTGCGGACATAACAGCTTTC+CGG + chr3.2:24606831-24606850 None:intergenic 45.0%
! GAAGTTTTTCGTGGATGACG+AGG - chr3.2:24607128-24607147 MS.gene38598:CDS 45.0%
!! CGACAAAAAGTCTGATTCGG+TGG - chr3.2:24606708-24606727 MS.gene38598:CDS 45.0%
ACTACCAGCTCTGCTTCCAA+CGG - chr3.2:24606892-24606911 MS.gene38598:CDS 50.0%
CAAATCCTTCCCTACTGGAC+AGG - chr3.2:24606948-24606967 MS.gene38598:CDS 50.0%
CATAACAGCTTTCCGGTGAC+TGG + chr3.2:24606824-24606843 None:intergenic 50.0%
GGCTTCTTTGCAGCAGGTAA+AGG + chr3.2:24607162-24607181 None:intergenic 50.0%
GGTGGTGATCTGATCTTCAG+CGG + chr3.2:24606857-24606876 None:intergenic 50.0%
TGACGATCCAACTCCTTCAG+AGG + chr3.2:24607052-24607071 None:intergenic 50.0%
TTCCCTACTGGACAGGCTTT+GGG - chr3.2:24606955-24606974 MS.gene38598:CDS 50.0%
! CACAAGCGTTGTCACTACGA+AGG - chr3.2:24606982-24607001 MS.gene38598:CDS 50.0%
! GTAACTGCCTCTGAAGGAGT+TGG - chr3.2:24607042-24607061 MS.gene38598:CDS 50.0%
! TCTTAACCTCCCGGCTTTTC+CGG - chr3.2:24607095-24607114 MS.gene38598:CDS 50.0%
!! ACTGGCTTTGTGTCCACCTA+GGG + chr3.2:24606806-24606825 None:intergenic 50.0%
!! GAAGCCCTGTCTTTTCAAGC+TGG - chr3.2:24607176-24607195 MS.gene38598:CDS 50.0%
AAAAGTCCGGAAAAGCCGGG+AGG + chr3.2:24607104-24607123 None:intergenic 55.0%
AGACAGGGCTTCTTTGCAGC+AGG + chr3.2:24607168-24607187 None:intergenic 55.0%
AGCGCTGTAACTGCCTCTGA+AGG - chr3.2:24607036-24607055 MS.gene38598:CDS 55.0%
CAAAGCCTGTCCAGTAGGGA+AGG + chr3.2:24606956-24606975 None:intergenic 55.0%
CTCATCATGCTTGCTCGCAG+CGG - chr3.2:24606676-24606695 MS.gene38598:CDS 55.0%
CTCCCAAAGCCTGTCCAGTA+GGG + chr3.2:24606960-24606979 None:intergenic 55.0%
CTGATCTTCAGCGGTGGTTG+CGG + chr3.2:24606848-24606867 None:intergenic 55.0%
CTTACCGTTGGAAGCAGAGC+TGG + chr3.2:24606899-24606918 None:intergenic 55.0%
CTTCCCTACTGGACAGGCTT+TGG - chr3.2:24606954-24606973 MS.gene38598:CDS 55.0%
GGAAGCAGAGCTGGTAGTCA+CGG + chr3.2:24606890-24606909 None:intergenic 55.0%
GGTGATCTGATCTTCAGCGG+TGG + chr3.2:24606854-24606873 None:intergenic 55.0%
GTGGACACAAAGCCAGTCAC+CGG - chr3.2:24606809-24606828 MS.gene38598:CDS 55.0%
TACAGCGCTACTTCCAGCAC+CGG + chr3.2:24607025-24607044 None:intergenic 55.0%
TGTCACTACGAAGGCAGCGT+TGG - chr3.2:24606991-24607010 MS.gene38598:CDS 55.0%
!! GACTGGCTTTGTGTCCACCT+AGG + chr3.2:24606807-24606826 None:intergenic 55.0%
AGCAGAGCTGGTAGTCACGG+CGG + chr3.2:24606887-24606906 None:intergenic 60.0%
AGTCACGGCGGATGAAGTGG+TGG + chr3.2:24606875-24606894 None:intergenic 60.0%
CCTCCCAAAGCCTGTCCAGT+AGG + chr3.2:24606961-24606980 None:intergenic 60.0%
GGTAGTCACGGCGGATGAAG+TGG + chr3.2:24606878-24606897 None:intergenic 60.0%
!! CCTACTGGACAGGCTTTGGG+AGG - chr3.2:24606958-24606977 MS.gene38598:CDS 60.0%
!! TACGAAGGCAGCGTTGGTGC+CGG - chr3.2:24606997-24607016 MS.gene38598:CDS 60.0%
!! GCTACTTCCAGCACCGGCAC+CGG + chr3.2:24607019-24607038 None:intergenic 65.0%
!! GGCAGCGTTGGTGCCGGTGC+CGG - chr3.2:24607003-24607022 MS.gene38598:CDS 75.0%
!! GTTGGTGCCGGTGCCGGTGC+TGG - chr3.2:24607009-24607028 MS.gene38598:CDS 75.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 24606631 24607218 24606631 ID=MS.gene38598
chr3.2 mRNA 24606631 24607218 24606631 ID=MS.gene38598.t1;Parent=MS.gene38598
chr3.2 exon 24606631 24607218 24606631 ID=MS.gene38598.t1.exon1;Parent=MS.gene38598.t1
chr3.2 CDS 24606631 24607218 24606631 ID=cds.MS.gene38598.t1;Parent=MS.gene38598.t1
Gene Sequence

>MS.gene38598

ATGGATCAATCTTCATGCACTGAAGAAGAGTATCTCGCTCTTTGTCTCATCATGCTTGCTCGCAGCGGTAACAACAACGACAAAAAGTCTGATTCGGTGGCGACGCCGCTAACCACCGTTAAACTCAGTCACAAATGCTCAGTCTGCAACAAAGCTTTCTCATCTTATCAAGCCCTAGGTGGACACAAAGCCAGTCACCGGAAAGCTGTTATGTCCGCAACCACCGCTGAAGATCAGATCACCACCACTTCATCCGCCGTGACTACCAGCTCTGCTTCCAACGGTAAGAACAAGACTCATGAGTGTTCCATCTGTCACAAATCCTTCCCTACTGGACAGGCTTTGGGAGGACACAAGCGTTGTCACTACGAAGGCAGCGTTGGTGCCGGTGCCGGTGCTGGAAGTAGCGCTGTAACTGCCTCTGAAGGAGTTGGATCGTCACACAGCCACCACCGTGATTTTGATCTTAACCTCCCGGCTTTTCCGGACTTTTCAAAGAAGTTTTTCGTGGATGACGAGGTTTCTAGTCCTTTACCTGCTGCAAAGAAGCCCTGTCTTTTCAAGCTGGAAATTCCTTCTCATTACTGA

Protein sequence

>MS.gene38598.t1

MDQSSCTEEEYLALCLIMLARSGNNNDKKSDSVATPLTTVKLSHKCSVCNKAFSSYQALGGHKASHRKAVMSATTAEDQITTTSSAVTTSSASNGKNKTHECSICHKSFPTGQALGGHKRCHYEGSVGAGAGAGSSAVTASEGVGSSHSHHRDFDLNLPAFPDFSKKFFVDDEVSSPLPAAKKPCLFKLEIPSHY