Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057590.t1 | RHN44295.1 | 62.1 | 103 | 19 | 2 | 80 | 182 | 92 | 174 | 6.60E-23 | 117.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057590.t1 | G7L237 | 62.1 | 103 | 19 | 2 | 80 | 182 | 118 | 200 | 4.8e-23 | 117.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055229 | MS.gene057590 | 0.80364 | 3.17E-49 | -1.69E-46 |
| MS.gene057590 | MS.gene057591 | 0.893699 | 4.93E-75 | -1.69E-46 |
| MS.gene057590 | MS.gene45357 | 0.82676 | 2.27E-54 | -1.69E-46 |
| MS.gene057590 | MS.gene49515 | 0.839837 | 1.25E-57 | -1.69E-46 |
| MS.gene057590 | MS.gene51454 | 0.806196 | 9.25E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057590.t1 | MTR_7g011390 | 58.974 | 195 | 21 | 4 | 27 | 182 | 26 | 200 | 6.45e-57 | 178 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATTTAACAATACATTAAA+TGG | 0.208866 | 7.2:+85987395 | None:intergenic |
| CTGGCTGCTGCTGGCATCCA+AGG | 0.246519 | 7.2:+85987455 | None:intergenic |
| ATCCACCGGCTGGCTGCTGC+TGG | 0.269951 | 7.2:+85987446 | None:intergenic |
| GTGAAGTTGTATCATCTTCA+TGG | 0.356415 | 7.2:+85987892 | None:intergenic |
| CAGTTTCTTCTTCTTAGACT+TGG | 0.357409 | 7.2:+85988695 | None:intergenic |
| TCTAAATTCACTCTGGTCAT+TGG | 0.364019 | 7.2:-85988274 | MS.gene057590:CDS |
| GTGCACATGCCAACACAATA+TGG | 0.384828 | 7.2:-85987858 | MS.gene057590:CDS |
| TGGCTGCTGCTGGCATCCAA+GGG | 0.406161 | 7.2:+85987456 | None:intergenic |
| ACCTCCTTGCCATATTGTGT+TGG | 0.459988 | 7.2:+85987849 | None:intergenic |
| GGCATGTGCACAGGTTGAAT+TGG | 0.460863 | 7.2:+85987870 | None:intergenic |
| GGATGCCAGCAGCAGCCAGC+CGG | 0.479702 | 7.2:-85987451 | MS.gene057590:CDS |
| CCGTTCGTTCCCATGGGTGG+AGG | 0.484615 | 7.2:-85988744 | None:intergenic |
| TATTGTGTTGGCATGTGCAC+AGG | 0.498215 | 7.2:+85987861 | None:intergenic |
| GCAGGCCCGCAAAGCAAAAG+AGG | 0.498850 | 7.2:-85988316 | MS.gene057590:CDS |
| TTCCCGTTCGTTCCCATGGG+TGG | 0.500541 | 7.2:-85988747 | None:intergenic |
| TGAAAATGATATGAAGCCCT+TGG | 0.509252 | 7.2:-85987472 | MS.gene057590:CDS |
| TGCCAGCAGCAGCCAGCCGG+TGG | 0.518060 | 7.2:-85987448 | MS.gene057590:CDS |
| TGTTGAGCTCATCCACCGGC+TGG | 0.547658 | 7.2:+85987436 | None:intergenic |
| AGCCTCTTCAGCTAATCGAC+GGG | 0.554287 | 7.2:+85988356 | None:intergenic |
| CTCTTGCTCTAAATTCACTC+TGG | 0.587930 | 7.2:-85988281 | MS.gene057590:CDS |
| AGCCCGTCGATTAGCTGAAG+AGG | 0.588284 | 7.2:-85988358 | MS.gene057590:CDS |
| GAGCCTCTTCAGCTAATCGA+CGG | 0.591168 | 7.2:+85988355 | None:intergenic |
| GACTTGTTGAGCTCATCCAC+CGG | 0.601518 | 7.2:+85987432 | None:intergenic |
| CATGCCAACACAATATGGCA+AGG | 0.661413 | 7.2:-85987853 | MS.gene057590:CDS |
| GCCAACACAATATGGCAAGG+AGG | 0.737684 | 7.2:-85987850 | MS.gene057590:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTAATTATTTATTTGC+AGG | - | chr7.2:85988640-85988659 | MS.gene057590:intron | 10.0% |
| !! | ATACTAAATGTTATGAAAAT+TGG | + | chr7.2:85987519-85987538 | None:intergenic | 15.0% |
| !! | ATGTTCTGTTATTGTTAATT+TGG | - | chr7.2:85987660-85987679 | MS.gene057590:intron | 20.0% |
| !!! | CATAGCTTTTCTTTAAAATT+GGG | - | chr7.2:85988515-85988534 | MS.gene057590:intron | 20.0% |
| !!! | TGTTATTGTTAATTTGGTTA+TGG | - | chr7.2:85987666-85987685 | MS.gene057590:intron | 20.0% |
| !!! | TTTTAACTAACTTGTTATGT+TGG | - | chr7.2:85987634-85987653 | MS.gene057590:intron | 20.0% |
| ! | TTCCTTACTTATAAGCAAAA+GGG | - | chr7.2:85988607-85988626 | MS.gene057590:intron | 25.0% |
| ! | TTTCCTTACTTATAAGCAAA+AGG | - | chr7.2:85988606-85988625 | MS.gene057590:intron | 25.0% |
| !! | GTTTTCTTAAAAATTCCTCT+AGG | - | chr7.2:85988142-85988161 | MS.gene057590:intron | 25.0% |
| !! | GTTTTCTTAAAAATTGCTCT+AGG | - | chr7.2:85987978-85987997 | MS.gene057590:intron | 25.0% |
| !! | TTTTGCTTATAAGTAAGGAA+AGG | + | chr7.2:85988607-85988626 | None:intergenic | 25.0% |
| !!! | ACTTGGATTTCTTTTTTGTT+CGG | + | chr7.2:85987433-85987452 | None:intergenic | 25.0% |
| !!! | CCAATTTTAAAGAAAAGCTA+TGG | + | chr7.2:85988517-85988536 | None:intergenic | 25.0% |
| !!! | CCATAGCTTTTCTTTAAAAT+TGG | - | chr7.2:85988514-85988533 | MS.gene057590:intron | 25.0% |
| !!! | GCAATTTTTAAGAAAACCTA+CGG | + | chr7.2:85987975-85987994 | None:intergenic | 25.0% |
| !!! | GGAATTTTTAAGAAAACCTA+CGG | + | chr7.2:85988139-85988158 | None:intergenic | 25.0% |
| AGATGAAAAGTGAATAGAAG+TGG | + | chr7.2:85988449-85988468 | None:intergenic | 30.0% | |
| CTTCTTACTAATACACCAAA+TGG | - | chr7.2:85988541-85988560 | MS.gene057590:intron | 30.0% | |
| GAATAGAAGTGGTATACTTA+TGG | + | chr7.2:85988438-85988457 | None:intergenic | 30.0% | |
| TTATGATCAGATTTATCGTC+AGG | - | chr7.2:85987602-85987621 | MS.gene057590:intron | 30.0% | |
| CAAAAAAGTAGCAGAAGAAG+AGG | - | chr7.2:85987760-85987779 | MS.gene057590:intron | 35.0% | |
| CAGTTTCTTCTTCTTAGACT+TGG | + | chr7.2:85987450-85987469 | None:intergenic | 35.0% | |
| GTGAAGTTGTATCATCTTCA+TGG | + | chr7.2:85988253-85988272 | None:intergenic | 35.0% | |
| TCAACTAATATCCAGACACT+AGG | + | chr7.2:85988390-85988409 | None:intergenic | 35.0% | |
| TCTAAATTCACTCTGGTCAT+TGG | - | chr7.2:85987868-85987887 | MS.gene057590:CDS | 35.0% | |
| TCTTGCTCAAAAAAAACTGC+AGG | - | chr7.2:85987808-85987827 | MS.gene057590:intron | 35.0% | |
| TGAAAATGATATGAAGCCCT+TGG | - | chr7.2:85988670-85988689 | MS.gene057590:CDS | 35.0% | |
| ! | AGCGTTTTTGTAGTCATGTT+TGG | + | chr7.2:85987920-85987939 | None:intergenic | 35.0% |
| ! | ATGGATTGACATGAATGTTC+AGG | + | chr7.2:85988419-85988438 | None:intergenic | 35.0% |
| ! | CATGATGATGATGATGATCT+TGG | - | chr7.2:85987705-85987724 | MS.gene057590:intron | 35.0% |
| ! | GAAAAAGCGTATCATCTTCA+TGG | + | chr7.2:85988089-85988108 | None:intergenic | 35.0% |
| ! | GCGTTTTTGTAGTCATGTTT+GGG | + | chr7.2:85987919-85987938 | None:intergenic | 35.0% |
| ! | GTCCCTTTTGCTTATAAGTA+AGG | + | chr7.2:85988612-85988631 | None:intergenic | 35.0% |
| ! | TTTTGTAATCTGTATGCAGC+TGG | - | chr7.2:85988062-85988081 | MS.gene057590:CDS | 35.0% |
| ! | TTTTGTAGTCATGTTTGGGT+AGG | + | chr7.2:85987915-85987934 | None:intergenic | 35.0% |
| !! | AAGCACGTTGTTTCTTAGTA+AGG | + | chr7.2:85988019-85988038 | None:intergenic | 35.0% |
| !! | CGTTGTTTCTTAGTAAGGAA+AGG | + | chr7.2:85988014-85988033 | None:intergenic | 35.0% |
| !!! | CTCTTCTTCTGCTACTTTTT+TGG | + | chr7.2:85987762-85987781 | None:intergenic | 35.0% |
| AAAGGACAAGACAATCCTAG+AGG | + | chr7.2:85987996-85988015 | None:intergenic | 40.0% | |
| AAATCTACAAACACAGCCGT+AGG | - | chr7.2:85987956-85987975 | MS.gene057590:intron | 40.0% | |
| AACAGGAGATAGGTTTATGC+AGG | + | chr7.2:85988357-85988376 | None:intergenic | 40.0% | |
| CAGTTTCATGAACAGGAGAT+AGG | + | chr7.2:85988367-85988386 | None:intergenic | 40.0% | |
| GATAAGAAGACAGTCCTAGA+GGG | + | chr7.2:85988585-85988604 | None:intergenic | 40.0% | |
| TGTTGTAATCTGTATGCAGC+TGG | - | chr7.2:85988226-85988245 | MS.gene057590:CDS | 40.0% | |
| ! | ATGATCTTGGCTCTCTGTAT+AGG | - | chr7.2:85987718-85987737 | MS.gene057590:intron | 40.0% |
| ! | CTCTTGCTCTAAATTCACTC+TGG | - | chr7.2:85987861-85987880 | MS.gene057590:CDS | 40.0% |
| AAAGCTACAAACACAGCCGT+AGG | - | chr7.2:85988120-85988139 | MS.gene057590:intron | 45.0% | |
| ACCTCCTTGCCATATTGTGT+TGG | + | chr7.2:85988296-85988315 | None:intergenic | 45.0% | |
| CACTAGGCAGTTTCATGAAC+AGG | + | chr7.2:85988374-85988393 | None:intergenic | 45.0% | |
| CATGCCAACACAATATGGCA+AGG | - | chr7.2:85988289-85988308 | MS.gene057590:CDS | 45.0% | |
| GGATAAGAAGACAGTCCTAG+AGG | + | chr7.2:85988586-85988605 | None:intergenic | 45.0% | |
| GTGCACATGCCAACACAATA+TGG | - | chr7.2:85988284-85988303 | MS.gene057590:CDS | 45.0% | |
| TCATGAAACTGCCTAGTGTC+TGG | - | chr7.2:85988376-85988395 | MS.gene057590:CDS | 45.0% | |
| ! | TATTGTGTTGGCATGTGCAC+AGG | + | chr7.2:85988284-85988303 | None:intergenic | 45.0% |
| !! | TACAAAATAAAAATAAAAAA+TGG | + | chr7.2:85987559-85987578 | None:intergenic | 5.0% |
| AGCCTCTTCAGCTAATCGAC+GGG | + | chr7.2:85987789-85987808 | None:intergenic | 50.0% | |
| CACCAAATGGAGAGAGCCTT+AGG | - | chr7.2:85988554-85988573 | MS.gene057590:intron | 50.0% | |
| GAGCCTCTTCAGCTAATCGA+CGG | + | chr7.2:85987790-85987809 | None:intergenic | 50.0% | |
| GCCAACACAATATGGCAAGG+AGG | - | chr7.2:85988292-85988311 | MS.gene057590:CDS | 50.0% | |
| TGCCTAAGGCTCTCTCCATT+TGG | + | chr7.2:85988559-85988578 | None:intergenic | 50.0% | |
| ! | CTGCTGCCTCTTTTGCTTTG+CGG | + | chr7.2:85987835-85987854 | None:intergenic | 50.0% |
| ! | GGCATGTGCACAGGTTGAAT+TGG | + | chr7.2:85988275-85988294 | None:intergenic | 50.0% |
| ! | TGCTGCCTCTTTTGCTTTGC+GGG | + | chr7.2:85987834-85987853 | None:intergenic | 50.0% |
| !! | GACTTGTTGAGCTCATCCAC+CGG | + | chr7.2:85988713-85988732 | None:intergenic | 50.0% |
| AGCCTTAGGCAATGCCCTCT+AGG | - | chr7.2:85988568-85988587 | MS.gene057590:intron | 55.0% | |
| ! | AGCCCGTCGATTAGCTGAAG+AGG | - | chr7.2:85987784-85987803 | MS.gene057590:intron | 55.0% |
| !! | GTCCTAGAGGGCATTGCCTA+AGG | + | chr7.2:85988573-85988592 | None:intergenic | 55.0% |
| GCAGGCCCGCAAAGCAAAAG+AGG | - | chr7.2:85987826-85987845 | MS.gene057590:intron | 60.0% | |
| TGGCTGCTGCTGGCATCCAA+GGG | + | chr7.2:85988689-85988708 | None:intergenic | 60.0% | |
| TGTTGAGCTCATCCACCGGC+TGG | + | chr7.2:85988709-85988728 | None:intergenic | 60.0% | |
| CTGGCTGCTGCTGGCATCCA+AGG | + | chr7.2:85988690-85988709 | None:intergenic | 65.0% | |
| GGATGCCAGCAGCAGCCAGC+CGG | - | chr7.2:85988691-85988710 | MS.gene057590:CDS | 70.0% | |
| ! | ATCCACCGGCTGGCTGCTGC+TGG | + | chr7.2:85988699-85988718 | None:intergenic | 70.0% |
| TGCCAGCAGCAGCCAGCCGG+TGG | - | chr7.2:85988694-85988713 | MS.gene057590:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 85987410 | 85988754 | 85987410 | ID=MS.gene057590 |
| chr7.2 | mRNA | 85987410 | 85988754 | 85987410 | ID=MS.gene057590.t1;Parent=MS.gene057590 |
| chr7.2 | exon | 85988659 | 85988754 | 85988659 | ID=MS.gene057590.t1.exon1;Parent=MS.gene057590.t1 |
| chr7.2 | CDS | 85988659 | 85988754 | 85988659 | ID=cds.MS.gene057590.t1;Parent=MS.gene057590.t1 |
| chr7.2 | exon | 85988191 | 85988424 | 85988191 | ID=MS.gene057590.t1.exon2;Parent=MS.gene057590.t1 |
| chr7.2 | CDS | 85988191 | 85988424 | 85988191 | ID=cds.MS.gene057590.t1;Parent=MS.gene057590.t1 |
| chr7.2 | exon | 85988027 | 85988083 | 85988027 | ID=MS.gene057590.t1.exon3;Parent=MS.gene057590.t1 |
| chr7.2 | CDS | 85988027 | 85988083 | 85988027 | ID=cds.MS.gene057590.t1;Parent=MS.gene057590.t1 |
| chr7.2 | exon | 85987851 | 85987919 | 85987851 | ID=MS.gene057590.t1.exon4;Parent=MS.gene057590.t1 |
| chr7.2 | CDS | 85987851 | 85987919 | 85987851 | ID=cds.MS.gene057590.t1;Parent=MS.gene057590.t1 |
| chr7.2 | exon | 85987410 | 85987502 | 85987410 | ID=MS.gene057590.t1.exon5;Parent=MS.gene057590.t1 |
| chr7.2 | CDS | 85987410 | 85987502 | 85987410 | ID=cds.MS.gene057590.t1;Parent=MS.gene057590.t1 |
| Gene Sequence |
| Protein sequence |
