Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45357.t1 | XP_003621282.2 | 72.3 | 213 | 21 | 3 | 26 | 220 | 25 | 217 | 1.70E-36 | 162.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45357.t1 | G7L237 | 72.3 | 213 | 21 | 3 | 26 | 220 | 25 | 217 | 1.2e-36 | 162.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057576 | MS.gene45357 | 0.88212 | 1.36E-70 | -1.69E-46 |
| MS.gene057577 | MS.gene45357 | 0.905241 | 5.28E-80 | -1.69E-46 |
| MS.gene057584 | MS.gene45357 | 0.882318 | 1.15E-70 | -1.69E-46 |
| MS.gene057586 | MS.gene45357 | 0.848958 | 4.40E-60 | -1.69E-46 |
| MS.gene057588 | MS.gene45357 | 0.916439 | 1.78E-85 | -1.69E-46 |
| MS.gene057590 | MS.gene45357 | 0.82676 | 2.27E-54 | -1.69E-46 |
| MS.gene057591 | MS.gene45357 | 0.916054 | 2.82E-85 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene45357.t1 | MTR_7g011390 | 76.050 | 238 | 18 | 3 | 1 | 220 | 1 | 217 | 4.22e-85 | 251 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGGCTGCTGCTGGCATCCA+AGG | 0.270786 | 7.3:+86336555 | None:intergenic |
| ATCCAACGGCTGGCTGCTGC+TGG | 0.310560 | 7.3:+86336546 | None:intergenic |
| CAGTTTCTTCTTCTTAGACT+TGG | 0.357409 | 7.3:+86337953 | None:intergenic |
| TCTAAATTCACTCTGGTCAT+TGG | 0.364019 | 7.3:-86337380 | MS.gene45357:CDS |
| GTGCACATGCCAACACAATA+TGG | 0.384828 | 7.3:-86336964 | MS.gene45357:CDS |
| TGAGGATAAAGCTGCTGTTC+AGG | 0.399727 | 7.3:-86336491 | MS.gene45357:CDS |
| TGGCTGCTGCTGGCATCCAA+GGG | 0.406161 | 7.3:+86336556 | None:intergenic |
| GTGAAGCTGTATCATCTTCA+TGG | 0.410616 | 7.3:+86336998 | None:intergenic |
| TTATGATCAGATTTATCATC+AGG | 0.427079 | 7.3:-86337813 | MS.gene45357:intron |
| GGTCCAATATCTAGAGTAGT+TGG | 0.437672 | 7.3:+86336449 | None:intergenic |
| GGCATGTGCACAGGTTGAAT+TGG | 0.460863 | 7.3:+86336976 | None:intergenic |
| ATAAAGCTGCTGTTCAGGAG+GGG | 0.477624 | 7.3:-86336486 | MS.gene45357:CDS |
| TGCCAGCAGCAGCCAGCCGT+TGG | 0.479830 | 7.3:-86336548 | MS.gene45357:CDS |
| CCGTTCGTTCCCATGGGTGG+AGG | 0.484615 | 7.3:-86338002 | None:intergenic |
| TATTGTGTTGGCATGTGCAC+AGG | 0.498215 | 7.3:+86336967 | None:intergenic |
| GCAGGCCCGCAAAGCAAAAG+AGG | 0.498850 | 7.3:-86337422 | MS.gene45357:CDS |
| TTCCCGTTCGTTCCCATGGG+TGG | 0.500541 | 7.3:-86338005 | None:intergenic |
| ACCACCTTGCCATATTGTGT+TGG | 0.505366 | 7.3:+86336955 | None:intergenic |
| TGAAAATGATATGAAGCCCT+TGG | 0.509252 | 7.3:-86336572 | MS.gene45357:CDS |
| GTCCAATATCTAGAGTAGTT+GGG | 0.512418 | 7.3:+86336450 | None:intergenic |
| TAAAGCTGCTGTTCAGGAGG+GGG | 0.518520 | 7.3:-86336485 | MS.gene45357:CDS |
| AGCCTCTTCTGCTAATCGAC+GGG | 0.530366 | 7.3:+86337516 | None:intergenic |
| GATAAAGCTGCTGTTCAGGA+GGG | 0.535418 | 7.3:-86336487 | MS.gene45357:CDS |
| TGTTGAGCTCATCCAACGGC+TGG | 0.538954 | 7.3:+86336536 | None:intergenic |
| TGCCCGTCGATTAGCAAAAG+AGG | 0.550604 | 7.3:-86337464 | MS.gene45357:CDS |
| AGCCCGTCGATTAGCAGAAG+AGG | 0.554670 | 7.3:-86337518 | MS.gene45357:CDS |
| CTCTTGCTCTAAATTCACTC+TGG | 0.587930 | 7.3:-86337387 | MS.gene45357:CDS |
| GAGCCTCTTCTGCTAATCGA+CGG | 0.589148 | 7.3:+86337515 | None:intergenic |
| GACTTGTTGAGCTCATCCAA+CGG | 0.593483 | 7.3:+86336532 | None:intergenic |
| AAAGCTGCTGTTCAGGAGGG+GGG | 0.608878 | 7.3:-86336484 | MS.gene45357:CDS |
| GGATAAAGCTGCTGTTCAGG+AGG | 0.611925 | 7.3:-86336488 | MS.gene45357:CDS |
| GAACAGCAGCTTTATCCTCA+AGG | 0.621167 | 7.3:+86336494 | None:intergenic |
| AACACAAAACGTTAGCCTTG+AGG | 0.622562 | 7.3:-86336509 | MS.gene45357:CDS |
| CATGCCAACACAATATGGCA+AGG | 0.666641 | 7.3:-86336959 | MS.gene45357:CDS |
| GCCAACACAATATGGCAAGG+TGG | 0.729546 | 7.3:-86336956 | MS.gene45357:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGATATGAATTGTTAATT+TGG | - | chr7.3:86336749-86336768 | MS.gene45357:intron | 15.0% |
| !! | TTATAAGAAATTAAGCAAAA+GGG | - | chr7.3:86337814-86337833 | MS.gene45357:CDS | 15.0% |
| !!! | ATTTTTAATTATGTATTTGC+AGG | - | chr7.3:86337847-86337866 | MS.gene45357:intron | 15.0% |
| !! | ATAAAATGATACTACTAATC+TGG | - | chr7.3:86336700-86336719 | MS.gene45357:intron | 20.0% |
| !! | CTAGTAAATGTTATGAAAAT+TGG | + | chr7.3:86336550-86336569 | None:intergenic | 20.0% |
| !! | CTTATAAGAAATTAAGCAAA+AGG | - | chr7.3:86337813-86337832 | MS.gene45357:intron | 20.0% |
| !! | TGCTTAATTTCTTATAAGTA+AGG | + | chr7.3:86337811-86337830 | None:intergenic | 20.0% |
| !!! | ATAAAAAAAAGTCTTGTAGA+AGG | + | chr7.3:86337523-86337542 | None:intergenic | 20.0% |
| !!! | ATGAATTGTTAATTTGGTTA+TGG | - | chr7.3:86336755-86336774 | MS.gene45357:intron | 20.0% |
| !!! | CATAGCTTTTCTTTAAAATT+GGG | - | chr7.3:86337720-86337739 | MS.gene45357:intron | 20.0% |
| !!! | TTTTAACTAACTTGTTATGT+TGG | - | chr7.3:86336668-86336687 | MS.gene45357:intron | 20.0% |
| ! | ACCTACAAATACAAATACAA+TGG | + | chr7.3:86336869-86336888 | None:intergenic | 25.0% |
| ! | TAAAAAAAAAGCAGAAGAAG+AGG | - | chr7.3:86336961-86336980 | MS.gene45357:CDS | 25.0% |
| ! | TCCATTGTATTTGTATTTGT+AGG | - | chr7.3:86336865-86336884 | MS.gene45357:intron | 25.0% |
| ! | TTATGATCAGATTTATCATC+AGG | - | chr7.3:86336636-86336655 | MS.gene45357:intron | 25.0% |
| !! | GTTTTCTTAAAAATTCCTCT+AGG | - | chr7.3:86337343-86337362 | MS.gene45357:CDS | 25.0% |
| !! | GTTTTCTTAAAAATTGCTCT+AGG | - | chr7.3:86337179-86337198 | MS.gene45357:intron | 25.0% |
| !!! | ACTTGGATTTCTTTTTTGTT+CGG | + | chr7.3:86336482-86336501 | None:intergenic | 25.0% |
| !!! | CCAATTTTAAAGAAAAGCTA+TGG | + | chr7.3:86337722-86337741 | None:intergenic | 25.0% |
| !!! | CCATAGCTTTTCTTTAAAAT+TGG | - | chr7.3:86337719-86337738 | MS.gene45357:intron | 25.0% |
| !!! | GCAATTTTTAAGAAAACCTA+CGG | + | chr7.3:86337176-86337195 | None:intergenic | 25.0% |
| !!! | GGAATTTTTAAGAAAACCTA+CGG | + | chr7.3:86337340-86337359 | None:intergenic | 25.0% |
| AATGATACTACTAATCTGGA+TGG | - | chr7.3:86336704-86336723 | MS.gene45357:intron | 30.0% | |
| AGATGAAAAGTGAATAGAAG+TGG | + | chr7.3:86337654-86337673 | None:intergenic | 30.0% | |
| ATTGATTGACATGAATGTTC+AGG | + | chr7.3:86337624-86337643 | None:intergenic | 30.0% | |
| ! | GATGATGATGATGATGATAT+TGG | - | chr7.3:86336797-86336816 | MS.gene45357:intron | 30.0% |
| AATATCCAGACACACACATT+AGG | + | chr7.3:86337589-86337608 | None:intergenic | 35.0% | |
| CAAAAAAGTAGCAGAAGAAG+AGG | - | chr7.3:86336907-86336926 | MS.gene45357:intron | 35.0% | |
| CAGTTTCTTCTTCTTAGACT+TGG | + | chr7.3:86336499-86336518 | None:intergenic | 35.0% | |
| CTTCTTGCTAATACACCAAA+TGG | - | chr7.3:86337746-86337765 | MS.gene45357:intron | 35.0% | |
| TCTAAATTCACTCTGGTCAT+TGG | - | chr7.3:86337069-86337088 | MS.gene45357:intron | 35.0% | |
| TCTTGCTCAAAAAAAACTGC+AGG | - | chr7.3:86337009-86337028 | MS.gene45357:intron | 35.0% | |
| TGAAAATGATATGAAGCCCT+TGG | - | chr7.3:86337877-86337896 | MS.gene45357:intron | 35.0% | |
| TGGAAAAGATAGATAGAGAG+AGG | + | chr7.3:86336530-86336549 | None:intergenic | 35.0% | |
| ! | AGCGTTTTTGTAGTCATGTT+TGG | + | chr7.3:86337121-86337140 | None:intergenic | 35.0% |
| ! | GAAAAAGCGTATCATCTTCA+TGG | + | chr7.3:86337290-86337309 | None:intergenic | 35.0% |
| ! | GCGTTTTTGTAGTCATGTTT+GGG | + | chr7.3:86337120-86337139 | None:intergenic | 35.0% |
| ! | TTTTGTAATCTGTATGCAGC+TGG | - | chr7.3:86337263-86337282 | MS.gene45357:intron | 35.0% |
| ! | TTTTGTAGTCATGTTTGGGT+AGG | + | chr7.3:86337116-86337135 | None:intergenic | 35.0% |
| !! | AAGCACGTTGTTTCTTAGTA+AGG | + | chr7.3:86337220-86337239 | None:intergenic | 35.0% |
| !! | ATGATATTGGCTCTCTGTAT+AGG | - | chr7.3:86336810-86336829 | MS.gene45357:intron | 35.0% |
| !! | CGTTGTTTCTTAGTAAGGAA+AGG | + | chr7.3:86337215-86337234 | None:intergenic | 35.0% |
| !!! | CTCTTCTTCTGCTACTTTTT+TGG | + | chr7.3:86336909-86336928 | None:intergenic | 35.0% |
| AAAGGACAAGACAATCCTAG+AGG | + | chr7.3:86337197-86337216 | None:intergenic | 40.0% | |
| AAATCTACAAACACAGCCGT+AGG | - | chr7.3:86337157-86337176 | MS.gene45357:CDS | 40.0% | |
| AACAGGAGATAGGTTTATGC+AGG | + | chr7.3:86337556-86337575 | None:intergenic | 40.0% | |
| CAGTTTCATGAACAGGAGAT+AGG | + | chr7.3:86337566-86337585 | None:intergenic | 40.0% | |
| CATTAGGCAGTTTCATGAAC+AGG | + | chr7.3:86337573-86337592 | None:intergenic | 40.0% | |
| GTGAAGCTGTATCATCTTCA+TGG | + | chr7.3:86337454-86337473 | None:intergenic | 40.0% | |
| ! | CTCTTGCTCTAAATTCACTC+TGG | - | chr7.3:86337062-86337081 | MS.gene45357:intron | 40.0% |
| !! | AACACAAAACGTTAGCCTTG+AGG | - | chr7.3:86337940-86337959 | MS.gene45357:intron | 40.0% |
| AAAGCTACAAACACAGCCGT+AGG | - | chr7.3:86337321-86337340 | MS.gene45357:CDS | 45.0% | |
| AACTGCCTAATGTGTGTGTC+TGG | - | chr7.3:86337581-86337600 | MS.gene45357:intron | 45.0% | |
| ACCACCTTGCCATATTGTGT+TGG | + | chr7.3:86337497-86337516 | None:intergenic | 45.0% | |
| AGGAAAGAAGACAGTCCTAG+AGG | + | chr7.3:86337791-86337810 | None:intergenic | 45.0% | |
| ATAAAGCTGCTGTTCAGGAG+GGG | - | chr7.3:86337963-86337982 | MS.gene45357:CDS | 45.0% | |
| CATGCCAACACAATATGGCA+AGG | - | chr7.3:86337490-86337509 | MS.gene45357:CDS | 45.0% | |
| GATAAAGCTGCTGTTCAGGA+GGG | - | chr7.3:86337962-86337981 | MS.gene45357:CDS | 45.0% | |
| GGAAAGAAGACAGTCCTAGA+GGG | + | chr7.3:86337790-86337809 | None:intergenic | 45.0% | |
| GTGCACATGCCAACACAATA+TGG | - | chr7.3:86337485-86337504 | MS.gene45357:CDS | 45.0% | |
| TGAGGATAAAGCTGCTGTTC+AGG | - | chr7.3:86337958-86337977 | MS.gene45357:CDS | 45.0% | |
| ! | AGCCTCTTTTGCTAATCGAC+GGG | + | chr7.3:86336990-86337009 | None:intergenic | 45.0% |
| ! | GAACAGCAGCTTTATCCTCA+AGG | + | chr7.3:86337958-86337977 | None:intergenic | 45.0% |
| ! | GAGCCTCTTTTGCTAATCGA+CGG | + | chr7.3:86336991-86337010 | None:intergenic | 45.0% |
| ! | TATTGTGTTGGCATGTGCAC+AGG | + | chr7.3:86337485-86337504 | None:intergenic | 45.0% |
| !! | GACTTGTTGAGCTCATCCAA+CGG | + | chr7.3:86337920-86337939 | None:intergenic | 45.0% |
| !! | TACAAAATAAAAATAAAAAA+TGG | + | chr7.3:86336593-86336612 | None:intergenic | 5.0% |
| AGCCTCTTCTGCTAATCGAC+GGG | + | chr7.3:86336936-86336955 | None:intergenic | 50.0% | |
| CACCAAATGGAGAGAGCCTT+AGG | - | chr7.3:86337759-86337778 | MS.gene45357:intron | 50.0% | |
| GAGCCTCTTCTGCTAATCGA+CGG | + | chr7.3:86336937-86336956 | None:intergenic | 50.0% | |
| GCCAACACAATATGGCAAGG+TGG | - | chr7.3:86337493-86337512 | MS.gene45357:CDS | 50.0% | |
| GGATAAAGCTGCTGTTCAGG+AGG | - | chr7.3:86337961-86337980 | MS.gene45357:CDS | 50.0% | |
| TAAAGCTGCTGTTCAGGAGG+GGG | - | chr7.3:86337964-86337983 | MS.gene45357:CDS | 50.0% | |
| TGCCTAAGGCTCTCTCCATT+TGG | + | chr7.3:86337764-86337783 | None:intergenic | 50.0% | |
| ! | CTGCTGCCTCTTTTGCTTTG+CGG | + | chr7.3:86337036-86337055 | None:intergenic | 50.0% |
| ! | GGCATGTGCACAGGTTGAAT+TGG | + | chr7.3:86337476-86337495 | None:intergenic | 50.0% |
| ! | TGCCCGTCGATTAGCAAAAG+AGG | - | chr7.3:86336985-86337004 | MS.gene45357:CDS | 50.0% |
| ! | TGCTGCCTCTTTTGCTTTGC+GGG | + | chr7.3:86337035-86337054 | None:intergenic | 50.0% |
| AAAGCTGCTGTTCAGGAGGG+GGG | - | chr7.3:86337965-86337984 | MS.gene45357:CDS | 55.0% | |
| AGCCTTAGGCAATGCCCTCT+AGG | - | chr7.3:86337773-86337792 | MS.gene45357:intron | 55.0% | |
| TGTTGAGCTCATCCAACGGC+TGG | + | chr7.3:86337916-86337935 | None:intergenic | 55.0% | |
| ! | AGCCCGTCGATTAGCAGAAG+AGG | - | chr7.3:86336931-86336950 | MS.gene45357:intron | 55.0% |
| !! | GTCCTAGAGGGCATTGCCTA+AGG | + | chr7.3:86337778-86337797 | None:intergenic | 55.0% |
| GCAGGCCCGCAAAGCAAAAG+AGG | - | chr7.3:86337027-86337046 | MS.gene45357:intron | 60.0% | |
| TGGCTGCTGCTGGCATCCAA+GGG | + | chr7.3:86337896-86337915 | None:intergenic | 60.0% | |
| ! | GCTGTTCAGGAGGGGGGTAT+TGG | - | chr7.3:86337971-86337990 | MS.gene45357:CDS | 60.0% |
| CTGGCTGCTGCTGGCATCCA+AGG | + | chr7.3:86337897-86337916 | None:intergenic | 65.0% | |
| ! | ATCCAACGGCTGGCTGCTGC+TGG | + | chr7.3:86337906-86337925 | None:intergenic | 65.0% |
| TGCCAGCAGCAGCCAGCCGT+TGG | - | chr7.3:86337901-86337920 | MS.gene45357:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 86336459 | 86338012 | 86336459 | ID=MS.gene45357 |
| chr7.3 | mRNA | 86336459 | 86338012 | 86336459 | ID=MS.gene45357.t1;Parent=MS.gene45357 |
| chr7.3 | exon | 86337952 | 86338012 | 86337952 | ID=MS.gene45357.t1.exon1;Parent=MS.gene45357.t1 |
| chr7.3 | CDS | 86337952 | 86338012 | 86337952 | ID=cds.MS.gene45357.t1;Parent=MS.gene45357.t1 |
| chr7.3 | exon | 86337814 | 86337857 | 86337814 | ID=MS.gene45357.t1.exon2;Parent=MS.gene45357.t1 |
| chr7.3 | CDS | 86337814 | 86337857 | 86337814 | ID=cds.MS.gene45357.t1;Parent=MS.gene45357.t1 |
| chr7.3 | exon | 86337297 | 86337584 | 86337297 | ID=MS.gene45357.t1.exon3;Parent=MS.gene45357.t1 |
| chr7.3 | CDS | 86337297 | 86337584 | 86337297 | ID=cds.MS.gene45357.t1;Parent=MS.gene45357.t1 |
| chr7.3 | exon | 86337133 | 86337189 | 86337133 | ID=MS.gene45357.t1.exon4;Parent=MS.gene45357.t1 |
| chr7.3 | CDS | 86337133 | 86337189 | 86337133 | ID=cds.MS.gene45357.t1;Parent=MS.gene45357.t1 |
| chr7.3 | exon | 86336957 | 86337025 | 86336957 | ID=MS.gene45357.t1.exon5;Parent=MS.gene45357.t1 |
| chr7.3 | CDS | 86336957 | 86337025 | 86336957 | ID=cds.MS.gene45357.t1;Parent=MS.gene45357.t1 |
| chr7.3 | exon | 86336459 | 86336602 | 86336459 | ID=MS.gene45357.t1.exon6;Parent=MS.gene45357.t1 |
| chr7.3 | CDS | 86336459 | 86336602 | 86336459 | ID=cds.MS.gene45357.t1;Parent=MS.gene45357.t1 |
| Gene Sequence |
| Protein sequence |
