Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057599.t1 | XP_003604693.2 | 99.2 | 257 | 1 | 1 | 1 | 256 | 168 | 424 | 9.30E-140 | 506.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057599.t1 | Q9H7Z3 | 32.3 | 167 | 93 | 3 | 93 | 248 | 293 | 450 | 5.5e-16 | 86.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057599.t1 | G7JP36 | 99.2 | 257 | 1 | 1 | 1 | 256 | 168 | 424 | 6.7e-140 | 506.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057598 | MS.gene057599 | 0.849305 | 3.52E-60 | -1.69E-46 |
| MS.gene057599 | MS.gene057600 | 0.909916 | 3.35E-82 | -1.69E-46 |
| MS.gene057599 | MS.gene057601 | 0.90493 | 7.33E-80 | -1.69E-46 |
| MS.gene057599 | MS.gene93340 | 0.809141 | 2.19E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAAATGTTAAATAAAACC+AGG | - | 33147:2641-2660 | MS.gene057599:CDS | 20.0% |
| !! | TGAAATGTTAAATAAAACCA+GGG | - | 33147:2642-2661 | MS.gene057599:CDS | 20.0% |
| !!! | TTGTTTGATAAGCTTTTAAA+AGG | + | 33147:2855-2874 | MS.gene057599:intergenic | 20.0% |
| ! | AAAATACTCTTACAACATTC+TGG | - | 33147:2910-2929 | MS.gene057599:CDS | 25.0% |
| ! | TGAAAAGAAAATTAGCATTC+TGG | - | 33147:2783-2802 | MS.gene057599:CDS | 25.0% |
| ! | TTTATACCTTTATCAGATGT+TGG | - | 33147:2550-2569 | MS.gene057599:CDS | 25.0% |
| !! | AGTAATAGATTTTCATTCTC+TGG | + | 33147:2829-2848 | MS.gene057599:intergenic | 25.0% |
| !! | GTAATAGATTTTCATTCTCT+GGG | + | 33147:2828-2847 | MS.gene057599:intergenic | 25.0% |
| !!! | TGATGATGAAATTTTAGAGT+CGG | + | 33147:2602-2621 | MS.gene057599:intergenic | 25.0% |
| AACCAAACTATTTCATCATG+CGG | + | 33147:2694-2713 | MS.gene057597:intergenic | 30.0% | |
| ATAATCCCTTGAATATGTCT+GGG | - | 33147:2326-2345 | MS.gene057599:CDS | 30.0% | |
| ATAGCTCAGATGTGTTAATT+GGG | - | 33147:2878-2897 | MS.gene057599:CDS | 30.0% | |
| ATCATCACTTGAAGAATCTT+GGG | - | 33147:2615-2634 | MS.gene057599:CDS | 30.0% | |
| ATGATAAAATGAAGTCTGCT+GGG | - | 33147:2425-2444 | MS.gene057599:CDS | 30.0% | |
| ATGTTAAAGGTTTGTATTGG+TGG | - | 33147:2353-2372 | MS.gene057599:CDS | 30.0% | |
| GACATATTCAAGGGATTATA+CGG | + | 33147:2325-2344 | MS.gene057599:intergenic | 30.0% | |
| GACATGTTAAAGGTTTGTAT+TGG | - | 33147:2350-2369 | MS.gene057599:CDS | 30.0% | |
| TATAATCCCTTGAATATGTC+TGG | - | 33147:2325-2344 | MS.gene057599:CDS | 30.0% | |
| ! | ATTCTGGAAGTTACAAGTTA+TGG | - | 33147:2926-2945 | MS.gene057599:CDS | 30.0% |
| !! | TTCTAAAGCCATATACTTTC+CGG | + | 33147:2461-2480 | MS.gene057599:intergenic | 30.0% |
| !!! | GATGATGAAATTTTAGAGTC+GGG | + | 33147:2601-2620 | MS.gene057599:intergenic | 30.0% |
| AACTCATCTTGTGTTGTATG+AGG | + | 33147:2532-2551 | MS.gene057597:intergenic | 35.0% | |
| AATTCTTGCATGTTGTTCAG+AGG | - | 33147:2953-2972 | MS.gene057599:CDS | 35.0% | |
| ACCAAACTATTTCATCATGC+GGG | + | 33147:2693-2712 | MS.gene057599:intergenic | 35.0% | |
| AGATGTGTTAATTGGGAGAT+GGG | - | 33147:2885-2904 | MS.gene057599:CDS | 35.0% | |
| CATCATCACTTGAAGAATCT+TGG | - | 33147:2614-2633 | MS.gene057599:CDS | 35.0% | |
| GATAGCTCAGATGTGTTAAT+TGG | - | 33147:2877-2896 | MS.gene057599:CDS | 35.0% | |
| GATGATAAAATGAAGTCTGC+TGG | - | 33147:2424-2443 | MS.gene057599:CDS | 35.0% | |
| GATGTACCAACATCTGATAA+AGG | + | 33147:2559-2578 | MS.gene057599:intergenic | 35.0% | |
| ! | GAAAATTAGCATTCTGGAGA+AGG | - | 33147:2789-2808 | MS.gene057599:CDS | 35.0% |
| ! | GTTGTATGAGGTGACAATTT+TGG | + | 33147:2520-2539 | MS.gene057599:intergenic | 35.0% |
| ! | TTGTATTGGTGGAATCAAAG+TGG | - | 33147:2364-2383 | MS.gene057599:CDS | 35.0% |
| AACCATGGAAACCTTGAACT+TGG | + | 33147:2986-3005 | MS.gene057597:intergenic | 40.0% | |
| ACCCGCATGATGAAATAGTT+TGG | - | 33147:2689-2708 | MS.gene057599:CDS | 40.0% | |
| AGATGGCGATATTGATGCTT+TGG | - | 33147:2402-2421 | MS.gene057599:CDS | 40.0% | |
| AGCATACATCTTCCTAACCA+TGG | + | 33147:3001-3020 | MS.gene057599:intergenic | 40.0% | |
| AGTTCAAGGTTTCCATGGTT+AGG | - | 33147:2986-3005 | MS.gene057599:CDS | 40.0% | |
| ATAAGGTTGCAGGAATGCAA+CGG | - | 33147:2734-2753 | MS.gene057599:CDS | 40.0% | |
| CAGATGTGTTAATTGGGAGA+TGG | - | 33147:2884-2903 | MS.gene057599:CDS | 40.0% | |
| GAATCAAAGTGGTTCACTTG+GGG | - | 33147:2375-2394 | MS.gene057599:CDS | 40.0% | |
| GAGGAATTTCTCCAAGTTCA+AGG | - | 33147:2972-2991 | MS.gene057599:CDS | 40.0% | |
| GGAATCAAAGTGGTTCACTT+GGG | - | 33147:2374-2393 | MS.gene057599:CDS | 40.0% | |
| GGAGTTCAACAAACTGACAA+GGG | - | 33147:2663-2682 | MS.gene057599:CDS | 40.0% | |
| TCAGATGTTGGTACATCTCA+AGG | - | 33147:2562-2581 | MS.gene057599:CDS | 40.0% | |
| TGCAACCTTATCTTGGAACT+CGG | + | 33147:2725-2744 | MS.gene057599:intergenic | 40.0% | |
| TGGAATCAAAGTGGTTCACT+TGG | - | 33147:2373-2392 | MS.gene057599:CDS | 40.0% | |
| ! | ATACTGGTCCGGAAAGTATA+TGG | - | 33147:2450-2469 | MS.gene057599:CDS | 40.0% |
| ! | ATTGAAGCTTTGTCTGCTTC+AGG | - | 33147:3027-3046 | MS.gene057599:CDS | 40.0% |
| ! | TTTTGCCGAGTTCCAAGATA+AGG | - | 33147:2717-2736 | MS.gene057599:CDS | 40.0% |
| !!! | ATTTTGGAGCAACAAGACGT+AGG | + | 33147:2504-2523 | MS.gene057599:intergenic | 40.0% |
| AGGGATTATACGGTTTGTGC+CGG | + | 33147:2315-2334 | MS.gene057599:intergenic | 45.0% | |
| ATGTCGCCCAGACATATTCA+AGG | + | 33147:2335-2354 | MS.gene057599:intergenic | 45.0% | |
| ATGTCTGGGCGACATGTTAA+AGG | - | 33147:2340-2359 | MS.gene057599:CDS | 45.0% | |
| CTCCAAGTTCAAGGTTTCCA+TGG | - | 33147:2981-3000 | MS.gene057599:CDS | 45.0% | |
| GAGTTCCAAGATAAGGTTGC+AGG | - | 33147:2724-2743 | MS.gene057599:CDS | 45.0% | |
| GCATTCCTGCAACCTTATCT+TGG | + | 33147:2732-2751 | MS.gene057599:intergenic | 45.0% | |
| GGGAGTTCAACAAACTGACA+AGG | - | 33147:2662-2681 | MS.gene057599:CDS | 45.0% | |
| TGTCGCCCAGACATATTCAA+GGG | + | 33147:2334-2353 | MS.gene057599:intergenic | 45.0% | |
| !! | TGTCAGTTTGTTGAACTCCC+TGG | + | 33147:2662-2681 | MS.gene057599:intergenic | 45.0% |
| GGGATTATACGGTTTGTGCC+GGG | + | 33147:2314-2333 | MS.gene057599:intergenic | 50.0% | |
| GGTTCACTTGGGGAAAGAGA+TGG | - | 33147:2385-2404 | MS.gene057599:CDS | 50.0% | |
| CTTCAGGCAGCAAGCACTCT+AGG | - | 33147:3043-3062 | MS.gene057599:CDS | 55.0% | |
| GCAGGAATGCAACGGCAGAA+AGG | - | 33147:2742-2761 | MS.gene057599:CDS | 55.0% | |
| TGAAGTCTGCTGGGCGATAC+TGG | - | 33147:2434-2453 | MS.gene057599:CDS | 55.0% | |
| TCTGCTGGGCGATACTGGTC+CGG | - | 33147:2439-2458 | MS.gene057599:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33147 | gene | 2300 | 3068 | 2300 | ID=MS.gene057599 |
| 33147 | mRNA | 2300 | 3068 | 2300 | ID=MS.gene057599.t1;Parent=MS.gene057599 |
| 33147 | exon | 2300 | 3068 | 2300 | ID=MS.gene057599.t1.exon1;Parent=MS.gene057599.t1 |
| 33147 | CDS | 2300 | 3068 | 2300 | ID=cds.MS.gene057599.t1;Parent=MS.gene057599.t1 |
| Gene Sequence |
| Protein sequence |
