Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057600.t1 | XP_003604693.2 | 99.1 | 213 | 1 | 1 | 1 | 212 | 212 | 424 | 1.30E-110 | 409.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057600.t1 | Q9H7Z3 | 32.3 | 167 | 93 | 3 | 49 | 204 | 293 | 450 | 4.6e-16 | 86.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057600.t1 | G7JP36 | 99.1 | 213 | 1 | 1 | 1 | 212 | 212 | 424 | 9.2e-111 | 409.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05350 | MS.gene057600 | 0.807877 | 4.08E-50 | -1.69E-46 |
| MS.gene057598 | MS.gene057600 | 0.864789 | 9.44E-65 | -1.69E-46 |
| MS.gene057599 | MS.gene057600 | 0.909916 | 3.35E-82 | -1.69E-46 |
| MS.gene057600 | MS.gene057601 | 0.871512 | 6.46E-67 | -1.69E-46 |
| MS.gene057600 | MS.gene057869 | 0.803064 | 4.17E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene058081 | 0.813255 | 2.81E-51 | -1.69E-46 |
| MS.gene057600 | MS.gene058919 | 0.807057 | 6.09E-50 | -1.69E-46 |
| MS.gene057600 | MS.gene062156 | 0.810374 | 1.19E-50 | -1.69E-46 |
| MS.gene057600 | MS.gene063209 | 0.803893 | 2.80E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene063772 | 0.808316 | 3.29E-50 | -1.69E-46 |
| MS.gene057600 | MS.gene069519 | 0.824968 | 6.05E-54 | -1.69E-46 |
| MS.gene057600 | MS.gene070713 | -0.806691 | 7.27E-50 | -1.69E-46 |
| MS.gene057600 | MS.gene072994 | 0.855782 | 5.01E-62 | -1.69E-46 |
| MS.gene057600 | MS.gene073132 | 0.815913 | 7.24E-52 | -1.69E-46 |
| MS.gene057600 | MS.gene073387 | 0.803762 | 2.99E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene073390 | 0.820138 | 8.04E-53 | -1.69E-46 |
| MS.gene057600 | MS.gene073727 | 0.821788 | 3.35E-53 | -1.69E-46 |
| MS.gene057600 | MS.gene07510 | 0.826763 | 2.27E-54 | -1.69E-46 |
| MS.gene057600 | MS.gene24203 | 0.813447 | 2.55E-51 | -1.69E-46 |
| MS.gene057600 | MS.gene28198 | 0.820748 | 5.82E-53 | -1.69E-46 |
| MS.gene057600 | MS.gene29041 | 0.805329 | 1.41E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene30301 | 0.807306 | 5.39E-50 | -1.69E-46 |
| MS.gene057600 | MS.gene30828 | 0.810273 | 1.25E-50 | -1.69E-46 |
| MS.gene057600 | MS.gene32884 | 0.805771 | 1.14E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene34065 | 0.839632 | 1.41E-57 | -1.69E-46 |
| MS.gene057600 | MS.gene34691 | 0.802258 | 6.11E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene36154 | 0.812804 | 3.53E-51 | -1.69E-46 |
| MS.gene057600 | MS.gene40068 | 0.801296 | 9.63E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene40518 | 0.803686 | 3.10E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene43430 | 0.812981 | 3.22E-51 | -1.69E-46 |
| MS.gene057600 | MS.gene43838 | 0.802421 | 5.66E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene43973 | 0.81955 | 1.10E-52 | -1.69E-46 |
| MS.gene057600 | MS.gene44621 | 0.803138 | 4.02E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene45732 | 0.80065 | 1.30E-48 | -1.69E-46 |
| MS.gene057600 | MS.gene50639 | 0.806103 | 9.68E-50 | -1.69E-46 |
| MS.gene057600 | MS.gene52437 | 0.804648 | 1.95E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene53064 | 0.800127 | 1.67E-48 | -1.69E-46 |
| MS.gene057600 | MS.gene56421 | 0.811186 | 7.94E-51 | -1.69E-46 |
| MS.gene057600 | MS.gene57127 | 0.800257 | 1.57E-48 | -1.69E-46 |
| MS.gene057600 | MS.gene59416 | 0.803724 | 3.04E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene63530 | 0.816121 | 6.51E-52 | -1.69E-46 |
| MS.gene057600 | MS.gene67893 | 0.810598 | 1.06E-50 | -1.69E-46 |
| MS.gene057600 | MS.gene68264 | 0.806944 | 6.43E-50 | -1.69E-46 |
| MS.gene057600 | MS.gene71202 | 0.810276 | 1.25E-50 | -1.69E-46 |
| MS.gene057600 | MS.gene74930 | 0.800423 | 1.45E-48 | -1.69E-46 |
| MS.gene057600 | MS.gene75866 | 0.823878 | 1.09E-53 | -1.69E-46 |
| MS.gene057600 | MS.gene79285 | 0.811034 | 8.57E-51 | -1.69E-46 |
| MS.gene057600 | MS.gene80094 | 0.81648 | 5.41E-52 | -1.69E-46 |
| MS.gene057600 | MS.gene83624 | 0.801768 | 7.70E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene84840 | 0.803048 | 4.20E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene85650 | 0.82802 | 1.13E-54 | -1.69E-46 |
| MS.gene057600 | MS.gene86826 | 0.820525 | 6.55E-53 | -1.69E-46 |
| MS.gene057600 | MS.gene87669 | 0.817633 | 2.98E-52 | -1.69E-46 |
| MS.gene057600 | MS.gene88649 | 0.800551 | 1.37E-48 | -1.69E-46 |
| MS.gene057600 | MS.gene90431 | 0.836634 | 8.34E-57 | -1.69E-46 |
| MS.gene057600 | MS.gene90585 | 0.803242 | 3.83E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene91208 | 0.803679 | 3.11E-49 | -1.69E-46 |
| MS.gene057600 | MS.gene91416 | 0.838058 | 3.60E-57 | -1.69E-46 |
| MS.gene057600 | MS.gene92340 | 0.821154 | 4.69E-53 | -1.69E-46 |
| MS.gene057600 | MS.gene93340 | 0.858402 | 8.45E-63 | -1.69E-46 |
| MS.gene057600 | MS.gene96210 | 0.837473 | 5.09E-57 | -1.69E-46 |
| MS.gene057600 | MS.gene99986 | 0.834122 | 3.60E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057600.t1 | MTR_4g016590 | 99.061 | 213 | 1 | 1 | 1 | 212 | 212 | 424 | 1.32e-143 | 429 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057600.t1 | AT3G17712 | 60.664 | 211 | 83 | 0 | 2 | 212 | 204 | 414 | 5.83e-76 | 244 |
| MS.gene057600.t1 | AT3G17740 | 60.664 | 211 | 83 | 0 | 2 | 212 | 204 | 414 | 2.49e-74 | 243 |
| MS.gene057600.t1 | AT3G17712 | 52.607 | 211 | 75 | 1 | 2 | 212 | 204 | 389 | 7.09e-58 | 195 |
| MS.gene057600.t1 | AT3G17712 | 52.607 | 211 | 75 | 1 | 2 | 212 | 235 | 420 | 1.75e-57 | 194 |
Find 0 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAAATGTTAAATAAAACC+AGG | - | 33150:1231-1250 | MS.gene057600:CDS | 20.0% |
| !! | TGAAATGTTAAATAAAACCA+GGG | - | 33150:1232-1251 | MS.gene057600:CDS | 20.0% |
| !!! | TTGTTTGATAAGCTTTTAAA+AGG | + | 33150:1445-1464 | MS.gene057600:intergenic | 20.0% |
| ! | AAAATACTCTTACAACATTC+TGG | - | 33150:1500-1519 | MS.gene057600:CDS | 25.0% |
| ! | TGAAAAGAAAATTAGCATTC+TGG | - | 33150:1373-1392 | MS.gene057600:CDS | 25.0% |
| ! | TTTATACCTTTATCAGATGT+TGG | - | 33150:1140-1159 | MS.gene057600:CDS | 25.0% |
| !! | AGTAATAGATTTTCATTCTC+TGG | + | 33150:1419-1438 | MS.gene057600:intergenic | 25.0% |
| !! | GTAATAGATTTTCATTCTCT+GGG | + | 33150:1418-1437 | MS.gene057600:intergenic | 25.0% |
| !!! | TGATGATGAAATTTTAGAGT+CGG | + | 33150:1192-1211 | MS.gene057600:intergenic | 25.0% |
| AACCAAACTATTTCATCATG+CGG | + | 33150:1284-1303 | MS.gene05760:intergenic | 30.0% | |
| ATAGCTCAGATGTGTTAATT+GGG | - | 33150:1468-1487 | MS.gene057600:CDS | 30.0% | |
| ATCATCACTTGAAGAATCTT+GGG | - | 33150:1205-1224 | MS.gene057600:CDS | 30.0% | |
| ! | ATTCTGGAAGTTACAAGTTA+TGG | - | 33150:1516-1535 | MS.gene057600:CDS | 30.0% |
| !! | TTCTAAAGCCATATACTTTC+CGG | + | 33150:1051-1070 | MS.gene057600:intergenic | 30.0% |
| !!! | GATGATGAAATTTTAGAGTC+GGG | + | 33150:1191-1210 | MS.gene057600:intergenic | 30.0% |
| AACTCATCTTGTGTTGTATG+AGG | + | 33150:1122-1141 | MS.gene05760:intergenic | 35.0% | |
| AATTCTTGCATGTTGTTCAG+AGG | - | 33150:1543-1562 | MS.gene057600:CDS | 35.0% | |
| ACCAAACTATTTCATCATGC+GGG | + | 33150:1283-1302 | MS.gene057600:intergenic | 35.0% | |
| AGATGTGTTAATTGGGAGAT+GGG | - | 33150:1475-1494 | MS.gene057600:CDS | 35.0% | |
| CATCATCACTTGAAGAATCT+TGG | - | 33150:1204-1223 | MS.gene057600:CDS | 35.0% | |
| GATAGCTCAGATGTGTTAAT+TGG | - | 33150:1467-1486 | MS.gene057600:CDS | 35.0% | |
| GATGTACCAACATCTGATAA+AGG | + | 33150:1149-1168 | MS.gene057600:intergenic | 35.0% | |
| ! | GAAAATTAGCATTCTGGAGA+AGG | - | 33150:1379-1398 | MS.gene057600:CDS | 35.0% |
| ! | GTTGTATGAGGTGACAATTT+TGG | + | 33150:1110-1129 | MS.gene057600:intergenic | 35.0% |
| AACCATGGAAACCTTGAACT+TGG | + | 33150:1576-1595 | MS.gene05760:intergenic | 40.0% | |
| ACCCGCATGATGAAATAGTT+TGG | - | 33150:1279-1298 | MS.gene057600:CDS | 40.0% | |
| AGCATACATCTTCCTAACCA+TGG | + | 33150:1591-1610 | MS.gene057600:intergenic | 40.0% | |
| AGTTCAAGGTTTCCATGGTT+AGG | - | 33150:1576-1595 | MS.gene057600:CDS | 40.0% | |
| ATAAGGTTGCAGGAATGCAA+CGG | - | 33150:1324-1343 | MS.gene057600:CDS | 40.0% | |
| CAGATGTGTTAATTGGGAGA+TGG | - | 33150:1474-1493 | MS.gene057600:CDS | 40.0% | |
| GAGGAATTTCTCCAAGTTCA+AGG | - | 33150:1562-1581 | MS.gene057600:CDS | 40.0% | |
| GGAGTTCAACAAACTGACAA+GGG | - | 33150:1253-1272 | MS.gene057600:CDS | 40.0% | |
| TCAGATGTTGGTACATCTCA+AGG | - | 33150:1152-1171 | MS.gene057600:CDS | 40.0% | |
| TGCAACCTTATCTTGGAACT+CGG | + | 33150:1315-1334 | MS.gene057600:intergenic | 40.0% | |
| ! | ATACTGGTCCGGAAAGTATA+TGG | - | 33150:1040-1059 | MS.gene057600:CDS | 40.0% |
| ! | ATTGAAGCTTTGTCTGCTTC+AGG | - | 33150:1617-1636 | MS.gene057600:CDS | 40.0% |
| ! | TTTTGCCGAGTTCCAAGATA+AGG | - | 33150:1307-1326 | MS.gene057600:CDS | 40.0% |
| !!! | ATTTTGGAGCAACAAGACGT+AGG | + | 33150:1094-1113 | MS.gene057600:intergenic | 40.0% |
| CTCCAAGTTCAAGGTTTCCA+TGG | - | 33150:1571-1590 | MS.gene057600:CDS | 45.0% | |
| GAGTTCCAAGATAAGGTTGC+AGG | - | 33150:1314-1333 | MS.gene057600:CDS | 45.0% | |
| GCATTCCTGCAACCTTATCT+TGG | + | 33150:1322-1341 | MS.gene057600:intergenic | 45.0% | |
| GGGAGTTCAACAAACTGACA+AGG | - | 33150:1252-1271 | MS.gene057600:CDS | 45.0% | |
| !! | TGTCAGTTTGTTGAACTCCC+TGG | + | 33150:1252-1271 | MS.gene057600:intergenic | 45.0% |
| CTTCAGGCAGCAAGCACTCT+AGG | - | 33150:1633-1652 | MS.gene057600:CDS | 55.0% | |
| GCAGGAATGCAACGGCAGAA+AGG | - | 33150:1332-1351 | MS.gene057600:CDS | 55.0% | |
| TGAAGTCTGCTGGGCGATAC+TGG | - | 33150:1024-1043 | MS.gene057600:CDS | 55.0% | |
| TCTGCTGGGCGATACTGGTC+CGG | - | 33150:1029-1048 | MS.gene057600:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33150 | gene | 1023 | 1658 | 1023 | ID=MS.gene057600 |
| 33150 | mRNA | 1023 | 1658 | 1023 | ID=MS.gene057600.t1;Parent=MS.gene057600 |
| 33150 | exon | 1023 | 1658 | 1023 | ID=MS.gene057600.t1.exon1;Parent=MS.gene057600.t1 |
| 33150 | CDS | 1023 | 1658 | 1023 | ID=cds.MS.gene057600.t1;Parent=MS.gene057600.t1 |
| Gene Sequence |
| Protein sequence |
