Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05769.t1 | XP_013460345.1 | 98 | 202 | 4 | 0 | 1 | 202 | 58 | 259 | 3.40E-105 | 391 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05769.t1 | F4J1Q9 | 54.0 | 200 | 92 | 0 | 1 | 200 | 32 | 231 | 8.4e-60 | 231.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05769.t1 | A0A072UYA2 | 98.0 | 202 | 4 | 0 | 1 | 202 | 58 | 259 | 2.5e-105 | 391.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050838 | MS.gene05769 | 0.812575 | 3.96E-51 | -1.69E-46 |
| MS.gene05769 | MS.gene072641 | 0.810352 | 1.20E-50 | -1.69E-46 |
| MS.gene05769 | MS.gene22160 | 0.825679 | 4.11E-54 | -1.69E-46 |
| MS.gene05769 | MS.gene28632 | 0.809772 | 1.60E-50 | -1.69E-46 |
| MS.gene05769 | MS.gene29891 | 0.822875 | 1.87E-53 | -1.69E-46 |
| MS.gene05769 | MS.gene33384 | 0.803461 | 3.45E-49 | -1.69E-46 |
| MS.gene05769 | MS.gene38070 | 0.820493 | 6.66E-53 | -1.69E-46 |
| MS.gene05769 | MS.gene49777 | 0.807899 | 4.03E-50 | -1.69E-46 |
| MS.gene05769 | MS.gene88859 | 0.832038 | 1.19E-55 | -1.69E-46 |
| MS.gene05769 | MS.gene92572 | 0.818201 | 2.22E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTAGAGAGTAGGAAGAAT+TGG | 0.296425 | 3.1:+49320952 | None:intergenic |
| TGGACCCAAAGGGAGATTAA+TGG | 0.351913 | 3.1:-49321337 | MS.gene05769:CDS |
| CAGGAAAGGTTCTGATATTA+AGG | 0.358857 | 3.1:+49321379 | None:intergenic |
| GATACCATTAATCTCCCTTT+GGG | 0.372516 | 3.1:+49321333 | None:intergenic |
| GAACTCTATCTTGCTGTTAC+CGG | 0.374940 | 3.1:-49321294 | MS.gene05769:CDS |
| ATTGATGGAACAGGGTTTCA+TGG | 0.377251 | 3.1:-49321048 | MS.gene05769:CDS |
| TGATACCATTAATCTCCCTT+TGG | 0.389795 | 3.1:+49321332 | None:intergenic |
| TTGTGGAATGGTGCAATTGA+TGG | 0.399316 | 3.1:-49321063 | MS.gene05769:CDS |
| ATACCTTATGCACTAGCATC+TGG | 0.414036 | 3.1:-49321489 | MS.gene05769:CDS |
| GACATAGGTCAACATGCATT+TGG | 0.427451 | 3.1:-49321357 | MS.gene05769:CDS |
| CCTCCAGATGCTAGTGCATA+AGG | 0.428913 | 3.1:+49321486 | None:intergenic |
| TCCTTCTAAAATAAGAAAAC+CGG | 0.435747 | 3.1:+49321275 | None:intergenic |
| CGTTCGGTACTAGTTTGTTA+AGG | 0.436402 | 3.1:+49321247 | None:intergenic |
| CACCTAGCAGGATTAACAAT+TGG | 0.437085 | 3.1:-49321216 | MS.gene05769:CDS |
| GCTAGGTGAATTTGCACGTT+CGG | 0.452957 | 3.1:+49321231 | None:intergenic |
| AATGGTGCAATTGATGGAAC+AGG | 0.475315 | 3.1:-49321057 | MS.gene05769:CDS |
| TGGATGAGCACTATAACAAA+AGG | 0.515129 | 3.1:+49320972 | None:intergenic |
| GGAACAGGGTTTCATGGTAA+AGG | 0.523162 | 3.1:-49321042 | MS.gene05769:CDS |
| AATGCATGTTGACCTATGTC+AGG | 0.538557 | 3.1:+49321360 | None:intergenic |
| TTGTTGTCTTATGTTTCTGC+CGG | 0.549030 | 3.1:-49321123 | MS.gene05769:CDS |
| CAGGGTTTCATGGTAAAGGA+AGG | 0.550822 | 3.1:-49321038 | MS.gene05769:CDS |
| AACGTGCAAATTCACCTAGC+AGG | 0.559395 | 3.1:-49321228 | MS.gene05769:CDS |
| CGATGAAGCTAAAGCACCAC+CGG | 0.563358 | 3.1:+49321104 | None:intergenic |
| CTAGCAGGATTAACAATTGG+TGG | 0.578790 | 3.1:-49321213 | MS.gene05769:CDS |
| GCCTGTGTAACAACATGCTA+TGG | 0.583300 | 3.1:+49321431 | None:intergenic |
| TTGTCTTATGTTTCTGCCGG+TGG | 0.592999 | 3.1:-49321120 | MS.gene05769:CDS |
| AGGGTTTCATGGTAAAGGAA+GGG | 0.593801 | 3.1:-49321037 | MS.gene05769:CDS |
| GCAAACAAACTAACAGCAGA+AGG | 0.594296 | 3.1:+49320994 | None:intergenic |
| CAACATGCATTTGGACCCAA+AGG | 0.594762 | 3.1:-49321348 | MS.gene05769:CDS |
| ATGCACTAGCATCTGGAGGA+TGG | 0.597399 | 3.1:-49321482 | MS.gene05769:CDS |
| GCCATAGCATGTTGTTACAC+AGG | 0.601297 | 3.1:-49321432 | MS.gene05769:CDS |
| ATCAGAACCTTTCCTGACAT+AGG | 0.626705 | 3.1:-49321372 | MS.gene05769:CDS |
| AACATGCATTTGGACCCAAA+GGG | 0.628001 | 3.1:-49321347 | MS.gene05769:CDS |
| ATGTCCTCTAACAAAACAGT+AGG | 0.628376 | 3.1:+49321150 | None:intergenic |
| CACCAATTGTTAATCCTGCT+AGG | 0.640186 | 3.1:+49321214 | None:intergenic |
| TACTCTCTACAACTCGATGA+GGG | 0.644461 | 3.1:-49320941 | MS.gene05769:CDS |
| ATGTTGACCTATGTCAGGAA+AGG | 0.648068 | 3.1:+49321365 | None:intergenic |
| CTACTCTCTACAACTCGATG+AGG | 0.655719 | 3.1:-49320942 | MS.gene05769:CDS |
| CCTTATGCACTAGCATCTGG+AGG | 0.658489 | 3.1:-49321486 | MS.gene05769:CDS |
| ATGTTGTTACACAGGCACAT+TGG | 0.661935 | 3.1:-49321424 | MS.gene05769:CDS |
| CTCATCGAGTTGTAGAGAGT+AGG | 0.667969 | 3.1:+49320943 | None:intergenic |
| ATGGTGCAATTGATGGAACA+GGG | 0.728483 | 3.1:-49321056 | MS.gene05769:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCCTTCTAAAATAAGAAAAC+CGG | + | chr3.1:49321155-49321174 | None:intergenic | 25.0% |
| !!! | ACCGGTTTTCTTATTTTAGA+AGG | - | chr3.1:49321151-49321170 | MS.gene05769:CDS | 30.0% |
| ATGTCCTCTAACAAAACAGT+AGG | + | chr3.1:49321280-49321299 | None:intergenic | 35.0% | |
| CACATTGGTCAAAAAATGCA+TGG | - | chr3.1:49321018-49321037 | MS.gene05769:CDS | 35.0% | |
| GATACCATTAATCTCCCTTT+GGG | + | chr3.1:49321097-49321116 | None:intergenic | 35.0% | |
| TGATACCATTAATCTCCCTT+TGG | + | chr3.1:49321098-49321117 | None:intergenic | 35.0% | |
| TGGATGAGCACTATAACAAA+AGG | + | chr3.1:49321458-49321477 | None:intergenic | 35.0% | |
| TTGTAGAGAGTAGGAAGAAT+TGG | + | chr3.1:49321478-49321497 | None:intergenic | 35.0% | |
| ! | ATCTTTTCGTTGTTGTGGAA+TGG | - | chr3.1:49321352-49321371 | MS.gene05769:CDS | 35.0% |
| ! | CAGGAAAGGTTCTGATATTA+AGG | + | chr3.1:49321051-49321070 | None:intergenic | 35.0% |
| ! | TCTTCATCTTTTCGTTGTTG+TGG | - | chr3.1:49321347-49321366 | MS.gene05769:CDS | 35.0% |
| ! | TTGTTGTCTTATGTTTCTGC+CGG | - | chr3.1:49321304-49321323 | MS.gene05769:CDS | 35.0% |
| !! | GTAAAGGAAGGGTTTTTAGA+TGG | - | chr3.1:49321401-49321420 | MS.gene05769:CDS | 35.0% |
| !! | TTTGCCTACTGTTTTGTTAG+AGG | - | chr3.1:49321273-49321292 | MS.gene05769:CDS | 35.0% |
| AACATGCATTTGGACCCAAA+GGG | - | chr3.1:49321080-49321099 | MS.gene05769:CDS | 40.0% | |
| AATGCATGTTGACCTATGTC+AGG | + | chr3.1:49321070-49321089 | None:intergenic | 40.0% | |
| AATGGTGCAATTGATGGAAC+AGG | - | chr3.1:49321370-49321389 | MS.gene05769:CDS | 40.0% | |
| AGGGTTTCATGGTAAAGGAA+GGG | - | chr3.1:49321390-49321409 | MS.gene05769:CDS | 40.0% | |
| ATACCTTATGCACTAGCATC+TGG | - | chr3.1:49320938-49320957 | MS.gene05769:CDS | 40.0% | |
| ATCAGAACCTTTCCTGACAT+AGG | - | chr3.1:49321055-49321074 | MS.gene05769:CDS | 40.0% | |
| ATGGTGCAATTGATGGAACA+GGG | - | chr3.1:49321371-49321390 | MS.gene05769:CDS | 40.0% | |
| ATGTTGACCTATGTCAGGAA+AGG | + | chr3.1:49321065-49321084 | None:intergenic | 40.0% | |
| ATGTTGTTACACAGGCACAT+TGG | - | chr3.1:49321003-49321022 | MS.gene05769:CDS | 40.0% | |
| ATTGATGGAACAGGGTTTCA+TGG | - | chr3.1:49321379-49321398 | MS.gene05769:CDS | 40.0% | |
| CACCAATTGTTAATCCTGCT+AGG | + | chr3.1:49321216-49321235 | None:intergenic | 40.0% | |
| CACCTAGCAGGATTAACAAT+TGG | - | chr3.1:49321211-49321230 | MS.gene05769:CDS | 40.0% | |
| CTAGCAGGATTAACAATTGG+TGG | - | chr3.1:49321214-49321233 | MS.gene05769:CDS | 40.0% | |
| GACATAGGTCAACATGCATT+TGG | - | chr3.1:49321070-49321089 | MS.gene05769:CDS | 40.0% | |
| GCAAACAAACTAACAGCAGA+AGG | + | chr3.1:49321436-49321455 | None:intergenic | 40.0% | |
| GGTCAAAAAATGCATGGACA+TGG | - | chr3.1:49321024-49321043 | MS.gene05769:CDS | 40.0% | |
| TACTCTCTACAACTCGATGA+GGG | - | chr3.1:49321486-49321505 | MS.gene05769:CDS | 40.0% | |
| TTGTGGAATGGTGCAATTGA+TGG | - | chr3.1:49321364-49321383 | MS.gene05769:CDS | 40.0% | |
| !! | CGTTCGGTACTAGTTTGTTA+AGG | + | chr3.1:49321183-49321202 | None:intergenic | 40.0% |
| !! | GAACTCTATCTTGCTGTTAC+CGG | - | chr3.1:49321133-49321152 | MS.gene05769:CDS | 40.0% |
| AACGTGCAAATTCACCTAGC+AGG | - | chr3.1:49321199-49321218 | MS.gene05769:CDS | 45.0% | |
| CAACATGCATTTGGACCCAA+AGG | - | chr3.1:49321079-49321098 | MS.gene05769:CDS | 45.0% | |
| CAGGGTTTCATGGTAAAGGA+AGG | - | chr3.1:49321389-49321408 | MS.gene05769:CDS | 45.0% | |
| CTACTCTCTACAACTCGATG+AGG | - | chr3.1:49321485-49321504 | MS.gene05769:CDS | 45.0% | |
| GCCATAGCATGTTGTTACAC+AGG | - | chr3.1:49320995-49321014 | MS.gene05769:CDS | 45.0% | |
| GCCTGTGTAACAACATGCTA+TGG | + | chr3.1:49320999-49321018 | None:intergenic | 45.0% | |
| GCTAGGTGAATTTGCACGTT+CGG | + | chr3.1:49321199-49321218 | None:intergenic | 45.0% | |
| GGAACAGGGTTTCATGGTAA+AGG | - | chr3.1:49321385-49321404 | MS.gene05769:CDS | 45.0% | |
| TGGACCCAAAGGGAGATTAA+TGG | - | chr3.1:49321090-49321109 | MS.gene05769:CDS | 45.0% | |
| ! | CTCATCGAGTTGTAGAGAGT+AGG | + | chr3.1:49321487-49321506 | None:intergenic | 45.0% |
| ! | TTGTCTTATGTTTCTGCCGG+TGG | - | chr3.1:49321307-49321326 | MS.gene05769:CDS | 45.0% |
| !! | GGAAGGGTTTTTAGATGGAG+TGG | - | chr3.1:49321406-49321425 | MS.gene05769:CDS | 45.0% |
| ATGCACTAGCATCTGGAGGA+TGG | - | chr3.1:49320945-49320964 | MS.gene05769:CDS | 50.0% | |
| CCTCCAGATGCTAGTGCATA+AGG | + | chr3.1:49320944-49320963 | None:intergenic | 50.0% | |
| CCTTATGCACTAGCATCTGG+AGG | - | chr3.1:49320941-49320960 | MS.gene05769:CDS | 50.0% | |
| ! | CGATGAAGCTAAAGCACCAC+CGG | + | chr3.1:49321326-49321345 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 49320921 | 49321528 | 49320921 | ID=MS.gene05769 |
| chr3.1 | mRNA | 49320921 | 49321528 | 49320921 | ID=MS.gene05769.t1;Parent=MS.gene05769 |
| chr3.1 | exon | 49320921 | 49321528 | 49320921 | ID=MS.gene05769.t1.exon1;Parent=MS.gene05769.t1 |
| chr3.1 | CDS | 49320921 | 49321528 | 49320921 | ID=cds.MS.gene05769.t1;Parent=MS.gene05769.t1 |
| Gene Sequence |
| Protein sequence |
