Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057758.t1 | RHN59945.1 | 70.7 | 123 | 31 | 3 | 1 | 121 | 4 | 123 | 6.30E-38 | 166.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057758.t1 | A0A396I8G4 | 70.7 | 123 | 31 | 3 | 1 | 121 | 4 | 123 | 4.6e-38 | 166.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055875 | MS.gene057758 | 0.829026 | 6.48E-55 | -1.69E-46 |
| MS.gene057656 | MS.gene057758 | 0.902013 | 1.50E-78 | -1.69E-46 |
| MS.gene057755 | MS.gene057758 | 0.889483 | 2.33E-73 | -1.69E-46 |
| MS.gene057757 | MS.gene057758 | 0.951307 | 2.69E-109 | -1.69E-46 |
| MS.gene057758 | MS.gene057760 | 0.931706 | 2.54E-94 | -1.69E-46 |
| MS.gene057758 | MS.gene057763 | 0.940876 | 1.10E-100 | -1.69E-46 |
| MS.gene057758 | MS.gene52126 | 0.934008 | 7.84E-96 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057758.t1 | MTR_4g069090 | 49.606 | 127 | 52 | 7 | 3 | 120 | 6 | 129 | 1.67e-22 | 91.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057758.t1 | AT3G18860 | 35.484 | 124 | 73 | 4 | 4 | 120 | 9 | 132 | 1.09e-16 | 75.1 |
| MS.gene057758.t1 | AT3G18860 | 35.484 | 124 | 73 | 4 | 4 | 120 | 9 | 132 | 1.09e-16 | 75.1 |
Find 33 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAAAAGCGGTGGTGTTTC+TGG | 0.222387 | 4.4:-36346886 | MS.gene057758:CDS |
| TGAATAATGCTGTGGTTGTT+TGG | 0.299775 | 4.4:-36346858 | MS.gene057758:CDS |
| CTTGTTTCATCTTCTATTGA+TGG | 0.317174 | 4.4:-36346757 | MS.gene057758:CDS |
| GTTACTGGTATTGTGGTTGA+TGG | 0.375152 | 4.4:-36346787 | MS.gene057758:CDS |
| GATCATGAATCGGAGGTTAC+TGG | 0.380364 | 4.4:-36346802 | MS.gene057758:CDS |
| GAATAATGCTGTGGTTGTTT+GGG | 0.416958 | 4.4:-36346857 | MS.gene057758:CDS |
| CTGACCAAATGATTAGGCTT+TGG | 0.426979 | 4.4:-36346999 | MS.gene057758:CDS |
| TTACTGGTATTGTGGTTGAT+GGG | 0.435643 | 4.4:-36346786 | MS.gene057758:CDS |
| ATACATCGGACGGTGCTTGC+GGG | 0.440853 | 4.4:+36346934 | None:intergenic |
| CTGTCCAAAGCCTAATCATT+TGG | 0.453917 | 4.4:+36346995 | None:intergenic |
| TCATCTTCTATTGATGGGTA+GGG | 0.456782 | 4.4:-36346751 | None:intergenic |
| TTGTTTCATCTTCTATTGAT+GGG | 0.457455 | 4.4:-36346756 | MS.gene057758:CDS |
| CCTCATCTGACCAAATGATT+AGG | 0.457665 | 4.4:-36347005 | MS.gene057758:CDS |
| TTCATCTTCTATTGATGGGT+AGG | 0.466392 | 4.4:-36346752 | None:intergenic |
| TACTGGTATTGTGGTTGATG+GGG | 0.482696 | 4.4:-36346785 | MS.gene057758:CDS |
| ATTAGGCTTTGGACAGTTCA+CGG | 0.484634 | 4.4:-36346988 | MS.gene057758:CDS |
| TTTGCCGGTGTTGCCGCAGA+TGG | 0.489699 | 4.4:+36347035 | None:intergenic |
| AAACACGATGCTGAAGTTTG+CGG | 0.506056 | 4.4:-36347063 | MS.gene057758:CDS |
| GCACCGTCCGATGTATATCA+TGG | 0.517069 | 4.4:-36346927 | MS.gene057758:CDS |
| GTTGTTTGGGACGTGGATAG+TGG | 0.528762 | 4.4:-36346844 | MS.gene057758:CDS |
| ATAGCCATCTGCGGCAACAC+CGG | 0.532590 | 4.4:-36347039 | MS.gene057758:CDS |
| TATACATCGGACGGTGCTTG+CGG | 0.535733 | 4.4:+36346933 | None:intergenic |
| TGCTGTGGTTGTTTGGGACG+TGG | 0.540919 | 4.4:-36346851 | MS.gene057758:CDS |
| GAAGACACTAGATCATGAAT+CGG | 0.571511 | 4.4:-36346812 | MS.gene057758:CDS |
| TGGTTGTGTGAATAATGCTG+TGG | 0.608311 | 4.4:-36346866 | MS.gene057758:CDS |
| CCTAATCATTTGGTCAGATG+AGG | 0.609733 | 4.4:+36347005 | None:intergenic |
| ATCGGAGGTTACTGGTATTG+TGG | 0.622916 | 4.4:-36346794 | MS.gene057758:CDS |
| AACCCTACTTTGAAAAGCGG+TGG | 0.631203 | 4.4:-36346895 | MS.gene057758:CDS |
| GACACTAGATCATGAATCGG+AGG | 0.635591 | 4.4:-36346809 | MS.gene057758:CDS |
| CCGAACCCTACTTTGAAAAG+CGG | 0.648212 | 4.4:-36346898 | MS.gene057758:CDS |
| AATCCATGATATACATCGGA+CGG | 0.656836 | 4.4:+36346924 | None:intergenic |
| GTGGAATCCATGATATACAT+CGG | 0.668837 | 4.4:+36346920 | None:intergenic |
| GTTTGCGGAATAGCCATCTG+CGG | 0.710293 | 4.4:-36347048 | MS.gene057758:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTGTTTCATCTTCTATTGAT+GGG | - | chr4.4:36347093-36347112 | MS.gene057758:CDS | 25.0% |
| CTTGTTTCATCTTCTATTGA+TGG | - | chr4.4:36347092-36347111 | MS.gene057758:CDS | 30.0% | |
| ! | TATCAAAAGATGTTTTCGAC+AGG | + | chr4.4:36346893-36346912 | None:intergenic | 30.0% |
| AATCCATGATATACATCGGA+CGG | + | chr4.4:36346928-36346947 | None:intergenic | 35.0% | |
| GAAGACACTAGATCATGAAT+CGG | - | chr4.4:36347037-36347056 | MS.gene057758:CDS | 35.0% | |
| GAATAATGCTGTGGTTGTTT+GGG | - | chr4.4:36346992-36347011 | MS.gene057758:CDS | 35.0% | |
| GTGGAATCCATGATATACAT+CGG | + | chr4.4:36346932-36346951 | None:intergenic | 35.0% | |
| TGAATAATGCTGTGGTTGTT+TGG | - | chr4.4:36346991-36347010 | MS.gene057758:CDS | 35.0% | |
| TTACTGGTATTGTGGTTGAT+GGG | - | chr4.4:36347063-36347082 | MS.gene057758:CDS | 35.0% | |
| AAACACGATGCTGAAGTTTG+CGG | - | chr4.4:36346786-36346805 | MS.gene057758:CDS | 40.0% | |
| CCTAATCATTTGGTCAGATG+AGG | + | chr4.4:36346847-36346866 | None:intergenic | 40.0% | |
| CCTCATCTGACCAAATGATT+AGG | - | chr4.4:36346844-36346863 | MS.gene057758:CDS | 40.0% | |
| CTGACCAAATGATTAGGCTT+TGG | - | chr4.4:36346850-36346869 | MS.gene057758:CDS | 40.0% | |
| CTGTCCAAAGCCTAATCATT+TGG | + | chr4.4:36346857-36346876 | None:intergenic | 40.0% | |
| GTTACTGGTATTGTGGTTGA+TGG | - | chr4.4:36347062-36347081 | MS.gene057758:CDS | 40.0% | |
| TGGTTGTGTGAATAATGCTG+TGG | - | chr4.4:36346983-36347002 | MS.gene057758:CDS | 40.0% | |
| ! | AGATGAGGTGACTATTTTGC+CGG | + | chr4.4:36346832-36346851 | None:intergenic | 40.0% |
| ! | TACTGGTATTGTGGTTGATG+GGG | - | chr4.4:36347064-36347083 | MS.gene057758:CDS | 40.0% |
| !! | ATTAGGCTTTGGACAGTTCA+CGG | - | chr4.4:36346861-36346880 | MS.gene057758:CDS | 40.0% |
| ATCGGAGGTTACTGGTATTG+TGG | - | chr4.4:36347055-36347074 | MS.gene057758:CDS | 45.0% | |
| GACACTAGATCATGAATCGG+AGG | - | chr4.4:36347040-36347059 | MS.gene057758:CDS | 45.0% | |
| GATCATGAATCGGAGGTTAC+TGG | - | chr4.4:36347047-36347066 | MS.gene057758:CDS | 45.0% | |
| TTGAAAAGCGGTGGTGTTTC+TGG | - | chr4.4:36346963-36346982 | MS.gene057758:CDS | 45.0% | |
| ! | AACCCTACTTTGAAAAGCGG+TGG | - | chr4.4:36346954-36346973 | MS.gene057758:CDS | 45.0% |
| ! | CCGAACCCTACTTTGAAAAG+CGG | - | chr4.4:36346951-36346970 | MS.gene057758:CDS | 45.0% |
| ! | CTTTTCAAAGTAGGGTTCGG+TGG | + | chr4.4:36346951-36346970 | None:intergenic | 45.0% |
| !! | ACACCACCGCTTTTCAAAGT+AGG | + | chr4.4:36346960-36346979 | None:intergenic | 45.0% |
| !! | CACCACCGCTTTTCAAAGTA+GGG | + | chr4.4:36346959-36346978 | None:intergenic | 45.0% |
| !! | CCGCTTTTCAAAGTAGGGTT+CGG | + | chr4.4:36346954-36346973 | None:intergenic | 45.0% |
| GCACCGTCCGATGTATATCA+TGG | - | chr4.4:36346922-36346941 | MS.gene057758:CDS | 50.0% | |
| GTTTGCGGAATAGCCATCTG+CGG | - | chr4.4:36346801-36346820 | MS.gene057758:CDS | 50.0% | |
| TATACATCGGACGGTGCTTG+CGG | + | chr4.4:36346919-36346938 | None:intergenic | 50.0% | |
| ! | GTTGTTTGGGACGTGGATAG+TGG | - | chr4.4:36347005-36347024 | MS.gene057758:CDS | 50.0% |
| ATACATCGGACGGTGCTTGC+GGG | + | chr4.4:36346918-36346937 | None:intergenic | 55.0% | |
| ATAGCCATCTGCGGCAACAC+CGG | - | chr4.4:36346810-36346829 | MS.gene057758:CDS | 55.0% | |
| ! | TGCTGTGGTTGTTTGGGACG+TGG | - | chr4.4:36346998-36347017 | MS.gene057758:CDS | 55.0% |
| !! | TTTGCCGGTGTTGCCGCAGA+TGG | + | chr4.4:36346817-36346836 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 36346753 | 36347118 | 36346753 | ID=MS.gene057758 |
| chr4.4 | mRNA | 36346753 | 36347118 | 36346753 | ID=MS.gene057758.t1;Parent=MS.gene057758 |
| chr4.4 | exon | 36346753 | 36347118 | 36346753 | ID=MS.gene057758.t1.exon1;Parent=MS.gene057758.t1 |
| chr4.4 | CDS | 36346753 | 36347118 | 36346753 | ID=cds.MS.gene057758.t1;Parent=MS.gene057758.t1 |
| Gene Sequence |
| Protein sequence |
