Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057755.t1 | RHN59945.1 | 57.1 | 389 | 109 | 5 | 5 | 392 | 6 | 337 | 3.70E-89 | 339 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057755.t1 | G5EES6 | 27.5 | 331 | 208 | 11 | 37 | 343 | 49 | 371 | 9.7e-06 | 53.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057755.t1 | A0A396I8G4 | 57.1 | 389 | 109 | 5 | 5 | 392 | 6 | 337 | 2.7e-89 | 339.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050442 | MS.gene057755 | 0.805884 | 1.08E-49 | -1.69E-46 |
| MS.gene051147 | MS.gene057755 | 0.808287 | 3.34E-50 | -1.69E-46 |
| MS.gene053998 | MS.gene057755 | 0.832876 | 7.36E-56 | -1.69E-46 |
| MS.gene055875 | MS.gene057755 | 0.911529 | 5.48E-83 | -1.69E-46 |
| MS.gene057656 | MS.gene057755 | 0.95406 | 6.90E-112 | -1.69E-46 |
| MS.gene057755 | MS.gene057756 | 0.884741 | 1.48E-71 | -1.69E-46 |
| MS.gene057755 | MS.gene057757 | 0.877348 | 6.76E-69 | -1.69E-46 |
| MS.gene057755 | MS.gene057758 | 0.889483 | 2.33E-73 | -1.69E-46 |
| MS.gene057755 | MS.gene057760 | 0.95249 | 2.17E-110 | -1.69E-46 |
| MS.gene057755 | MS.gene057763 | 0.869636 | 2.67E-66 | -1.69E-46 |
| MS.gene057755 | MS.gene060167 | 0.935844 | 4.46E-97 | -1.69E-46 |
| MS.gene057755 | MS.gene071033 | 0.810385 | 1.18E-50 | -1.69E-46 |
| MS.gene057755 | MS.gene08589 | 0.815903 | 7.28E-52 | -1.69E-46 |
| MS.gene057755 | MS.gene22076 | 0.806884 | 6.62E-50 | -1.69E-46 |
| MS.gene057755 | MS.gene23656 | 0.808217 | 3.45E-50 | -1.69E-46 |
| MS.gene057755 | MS.gene31231 | 0.802513 | 5.41E-49 | -1.69E-46 |
| MS.gene057755 | MS.gene31283 | 0.801992 | 6.93E-49 | -1.69E-46 |
| MS.gene057755 | MS.gene33624 | 0.810464 | 1.14E-50 | -1.69E-46 |
| MS.gene057755 | MS.gene34667 | 0.802173 | 6.36E-49 | -1.69E-46 |
| MS.gene057755 | MS.gene34700 | 0.854725 | 1.02E-61 | -1.69E-46 |
| MS.gene057755 | MS.gene39666 | 0.801201 | 1.01E-48 | -1.69E-46 |
| MS.gene057755 | MS.gene45422 | 0.801744 | 7.79E-49 | -1.69E-46 |
| MS.gene057755 | MS.gene46250 | 0.827713 | 1.34E-54 | -1.69E-46 |
| MS.gene057755 | MS.gene48704 | 0.82921 | 5.85E-55 | -1.69E-46 |
| MS.gene057755 | MS.gene51139 | 0.805471 | 1.31E-49 | -1.69E-46 |
| MS.gene057755 | MS.gene52126 | 0.852094 | 5.78E-61 | -1.69E-46 |
| MS.gene057755 | MS.gene52525 | 0.805423 | 1.34E-49 | -1.69E-46 |
| MS.gene057755 | MS.gene53064 | 0.804225 | 2.39E-49 | -1.69E-46 |
| MS.gene057755 | MS.gene54564 | 0.829958 | 3.85E-55 | -1.69E-46 |
| MS.gene057755 | MS.gene70231 | 0.800717 | 1.26E-48 | -1.69E-46 |
| MS.gene057755 | MS.gene71662 | 0.808157 | 3.56E-50 | -1.69E-46 |
| MS.gene057755 | MS.gene77715 | 0.803437 | 3.49E-49 | -1.69E-46 |
| MS.gene057755 | MS.gene88091 | 0.819586 | 1.07E-52 | -1.69E-46 |
| MS.gene057755 | MS.gene88112 | 0.830133 | 3.49E-55 | -1.69E-46 |
| MS.gene057755 | MS.gene90823 | 0.803268 | 3.78E-49 | -1.69E-46 |
| MS.gene057755 | MS.gene98087 | 0.820869 | 5.46E-53 | -1.69E-46 |
| MS.gene057755 | MS.gene98088 | 0.83189 | 1.29E-55 | -1.69E-46 |
| MS.gene057755 | MS.gene99746 | 0.805153 | 1.53E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057755.t1 | MTR_4g069090 | 56.208 | 443 | 128 | 12 | 1 | 390 | 3 | 432 | 5.59e-145 | 433 |
| MS.gene057755.t1 | MTR_4g119620 | 31.128 | 257 | 145 | 15 | 20 | 258 | 81 | 323 | 3.17e-18 | 85.9 |
| MS.gene057755.t1 | MTR_2g012630 | 26.848 | 257 | 160 | 13 | 26 | 266 | 20 | 264 | 1.76e-12 | 70.5 |
| MS.gene057755.t1 | MTR_2g012630 | 26.848 | 257 | 160 | 13 | 26 | 266 | 20 | 264 | 1.85e-12 | 70.5 |
| MS.gene057755.t1 | MTR_2g102277 | 27.778 | 252 | 150 | 11 | 62 | 294 | 18 | 256 | 8.13e-12 | 68.2 |
| MS.gene057755.t1 | MTR_2g102277 | 27.778 | 252 | 150 | 11 | 62 | 294 | 18 | 256 | 1.07e-11 | 67.8 |
| MS.gene057755.t1 | MTR_4g095058 | 26.374 | 273 | 165 | 11 | 35 | 286 | 306 | 563 | 4.72e-11 | 65.5 |
| MS.gene057755.t1 | MTR_4g095058 | 26.374 | 273 | 165 | 11 | 35 | 286 | 308 | 565 | 5.12e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057755.t1 | AT3G18860 | 45.261 | 422 | 170 | 9 | 1 | 371 | 5 | 416 | 1.26e-109 | 342 |
| MS.gene057755.t1 | AT3G18860 | 45.261 | 422 | 170 | 9 | 1 | 371 | 5 | 416 | 1.31e-109 | 342 |
| MS.gene057755.t1 | AT3G49660 | 29.412 | 255 | 156 | 13 | 17 | 258 | 71 | 314 | 2.21e-19 | 89.4 |
| MS.gene057755.t1 | AT1G79990 | 26.718 | 262 | 160 | 11 | 52 | 294 | 8 | 256 | 1.43e-11 | 67.4 |
| MS.gene057755.t1 | AT1G79990 | 26.718 | 262 | 160 | 11 | 52 | 294 | 8 | 256 | 1.49e-11 | 67.4 |
| MS.gene057755.t1 | AT4G02730 | 27.311 | 238 | 149 | 11 | 35 | 258 | 104 | 331 | 7.06e-11 | 63.9 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33352 | gene | 15507 | 25808 | 15507 | ID=MS.gene057755 |
| 33352 | mRNA | 15507 | 25808 | 15507 | ID=MS.gene057755.t1;Parent=MS.gene057755 |
| 33352 | exon | 15507 | 15877 | 15507 | ID=MS.gene057755.t1.exon1;Parent=MS.gene057755.t1 |
| 33352 | CDS | 15507 | 15877 | 15507 | ID=cds.MS.gene057755.t1;Parent=MS.gene057755.t1 |
| 33352 | exon | 16406 | 16518 | 16406 | ID=MS.gene057755.t1.exon2;Parent=MS.gene057755.t1 |
| 33352 | CDS | 16406 | 16518 | 16406 | ID=cds.MS.gene057755.t1;Parent=MS.gene057755.t1 |
| 33352 | exon | 16658 | 16744 | 16658 | ID=MS.gene057755.t1.exon3;Parent=MS.gene057755.t1 |
| 33352 | CDS | 16658 | 16744 | 16658 | ID=cds.MS.gene057755.t1;Parent=MS.gene057755.t1 |
| 33352 | exon | 17045 | 17105 | 17045 | ID=MS.gene057755.t1.exon4;Parent=MS.gene057755.t1 |
| 33352 | CDS | 17045 | 17105 | 17045 | ID=cds.MS.gene057755.t1;Parent=MS.gene057755.t1 |
| 33352 | exon | 17212 | 17351 | 17212 | ID=MS.gene057755.t1.exon5;Parent=MS.gene057755.t1 |
| 33352 | CDS | 17212 | 17351 | 17212 | ID=cds.MS.gene057755.t1;Parent=MS.gene057755.t1 |
| 33352 | exon | 18586 | 18797 | 18586 | ID=MS.gene057755.t1.exon6;Parent=MS.gene057755.t1 |
| 33352 | CDS | 18586 | 18797 | 18586 | ID=cds.MS.gene057755.t1;Parent=MS.gene057755.t1 |
| 33352 | exon | 18913 | 18982 | 18913 | ID=MS.gene057755.t1.exon7;Parent=MS.gene057755.t1 |
| 33352 | CDS | 18913 | 18982 | 18913 | ID=cds.MS.gene057755.t1;Parent=MS.gene057755.t1 |
| 33352 | exon | 19072 | 19128 | 19072 | ID=MS.gene057755.t1.exon8;Parent=MS.gene057755.t1 |
| 33352 | CDS | 19072 | 19128 | 19072 | ID=cds.MS.gene057755.t1;Parent=MS.gene057755.t1 |
| 33352 | exon | 21307 | 21360 | 21307 | ID=MS.gene057755.t1.exon9;Parent=MS.gene057755.t1 |
| 33352 | CDS | 21307 | 21360 | 21307 | ID=cds.MS.gene057755.t1;Parent=MS.gene057755.t1 |
| 33352 | exon | 22024 | 22101 | 22024 | ID=MS.gene057755.t1.exon10;Parent=MS.gene057755.t1 |
| 33352 | CDS | 22024 | 22101 | 22024 | ID=cds.MS.gene057755.t1;Parent=MS.gene057755.t1 |
| 33352 | exon | 22482 | 22535 | 22482 | ID=MS.gene057755.t1.exon11;Parent=MS.gene057755.t1 |
| 33352 | CDS | 22482 | 22535 | 22482 | ID=cds.MS.gene057755.t1;Parent=MS.gene057755.t1 |
| 33352 | exon | 23182 | 23259 | 23182 | ID=MS.gene057755.t1.exon12;Parent=MS.gene057755.t1 |
| 33352 | CDS | 23182 | 23259 | 23182 | ID=cds.MS.gene057755.t1;Parent=MS.gene057755.t1 |
| 33352 | exon | 25740 | 25808 | 25740 | ID=MS.gene057755.t1.exon13;Parent=MS.gene057755.t1 |
| 33352 | CDS | 25740 | 25808 | 25740 | ID=cds.MS.gene057755.t1;Parent=MS.gene057755.t1 |
| Gene Sequence |
| Protein sequence |
