Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52126.t1 | RHN59945.1 | 73.7 | 152 | 33 | 3 | 5 | 155 | 6 | 151 | 1.20E-56 | 229.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52126.t1 | A0A396I8G4 | 73.7 | 152 | 33 | 3 | 5 | 155 | 6 | 151 | 8.7e-57 | 229.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055875 | MS.gene52126 | 0.831658 | 1.47E-55 | -1.69E-46 |
| MS.gene057656 | MS.gene52126 | 0.896388 | 3.87E-76 | -1.69E-46 |
| MS.gene057755 | MS.gene52126 | 0.852094 | 5.78E-61 | -1.69E-46 |
| MS.gene057757 | MS.gene52126 | 0.919734 | 3.11E-87 | -1.69E-46 |
| MS.gene057758 | MS.gene52126 | 0.934008 | 7.84E-96 | -1.69E-46 |
| MS.gene057760 | MS.gene52126 | 0.877231 | 7.42E-69 | -1.69E-46 |
| MS.gene057763 | MS.gene52126 | 0.898203 | 6.68E-77 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52126.t1 | MTR_4g069090 | 55.882 | 170 | 60 | 8 | 1 | 162 | 3 | 165 | 3.48e-44 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52126.t1 | AT3G18860 | 41.667 | 168 | 88 | 5 | 1 | 162 | 5 | 168 | 1.49e-34 | 129 |
| MS.gene52126.t1 | AT3G18860 | 41.667 | 168 | 88 | 5 | 1 | 162 | 5 | 168 | 1.49e-34 | 129 |
Find 50 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAAAAGCGGTGGTGTTTC+TGG | 0.222387 | 4.1:+29450948 | MS.gene52126:CDS |
| ATTTGAAGAGGTGGAGTAAT+TGG | 0.238721 | 4.1:+29451630 | MS.gene52126:CDS |
| TGAATAATGCTGTGGTTGTT+TGG | 0.299775 | 4.1:+29450976 | MS.gene52126:CDS |
| GGCGGAGTGCACAGCGTAAT+TGG | 0.307415 | 4.1:-29450749 | None:intergenic |
| CTTGTTTCATCTTCTATTGA+TGG | 0.317174 | 4.1:+29451077 | MS.gene52126:CDS |
| AGCTTTATAACTGCTTGAAT+TGG | 0.341084 | 4.1:-29451695 | None:intergenic |
| CACTCAGAATTATGTCTCTT+AGG | 0.345912 | 4.1:+29451790 | MS.gene52126:CDS |
| CCCACAGGAGAGCTTGTTAC+AGG | 0.356136 | 4.1:+29451719 | MS.gene52126:CDS |
| GTTACTGGTATTGTGGTTGA+TGG | 0.375152 | 4.1:+29451047 | MS.gene52126:CDS |
| GATCATGAATCGGAGGTTAC+TGG | 0.380364 | 4.1:+29451032 | MS.gene52126:CDS |
| GAATAATGCTGTGGTTGTTT+GGG | 0.416958 | 4.1:+29450977 | MS.gene52126:CDS |
| CCTCATCTGACCGAATGATT+AGG | 0.433114 | 4.1:+29450829 | MS.gene52126:CDS |
| TTACTGGTATTGTGGTTGAT+GGG | 0.435643 | 4.1:+29451048 | MS.gene52126:CDS |
| GTTCGAATTGCACGGAGACA+TGG | 0.436264 | 4.1:+29451657 | MS.gene52126:CDS |
| ATACATCGGACGGTGCTTGC+GGG | 0.440853 | 4.1:-29450900 | None:intergenic |
| TTGTTTCATCTTCTATTGAT+GGG | 0.457455 | 4.1:+29451078 | MS.gene52126:CDS |
| CTGACCGAATGATTAGGCTT+TGG | 0.468286 | 4.1:+29450835 | MS.gene52126:CDS |
| TACTGGTATTGTGGTTGATG+GGG | 0.482696 | 4.1:+29451049 | MS.gene52126:CDS |
| CACGATGATGATGAAGTTTG+CGG | 0.484126 | 4.1:+29450771 | MS.gene52126:CDS |
| ATTAGGCTTTGGACAGTTCA+CGG | 0.484634 | 4.1:+29450846 | MS.gene52126:CDS |
| TTTGCCGGTGTTGCCGCAGA+TGG | 0.489699 | 4.1:-29450799 | None:intergenic |
| CTGTCCAAAGCCTAATCATT+CGG | 0.499408 | 4.1:-29450839 | None:intergenic |
| GCACCGTCCGATGTATATCA+TGG | 0.517069 | 4.1:+29450907 | MS.gene52126:CDS |
| GTTGTTTGGGACGTGGATAG+TGG | 0.528762 | 4.1:+29450990 | MS.gene52126:CDS |
| ATAGCCATCTGCGGCAACAC+CGG | 0.532590 | 4.1:+29450795 | MS.gene52126:CDS |
| GGAGACATGGGCGGCTCACG+AGG | 0.534589 | 4.1:+29451670 | MS.gene52126:CDS |
| TATACATCGGACGGTGCTTG+CGG | 0.535733 | 4.1:-29450901 | None:intergenic |
| TGTGCAGTAATTTGAAGAGG+TGG | 0.537765 | 4.1:+29451621 | MS.gene52126:intron |
| TGCTGTGGTTGTTTGGGACG+TGG | 0.540919 | 4.1:+29450983 | MS.gene52126:CDS |
| TTGGAATTGTTCGAATTGCA+CGG | 0.545602 | 4.1:+29451649 | MS.gene52126:CDS |
| ACCTGTAACAAGCTCTCCTG+TGG | 0.546432 | 4.1:-29451720 | None:intergenic |
| TTCGAATTGCACGGAGACAT+GGG | 0.566298 | 4.1:+29451658 | MS.gene52126:CDS |
| GAAGACACTAGATCATGAAT+CGG | 0.571511 | 4.1:+29451022 | MS.gene52126:CDS |
| TCTTGTGCAGTAATTTGAAG+AGG | 0.591310 | 4.1:+29451618 | MS.gene52126:intron |
| TGGTTGTGTGAATAATGCTG+TGG | 0.608311 | 4.1:+29450968 | MS.gene52126:CDS |
| TCTGAGTGTAACCCAACAAG+TGG | 0.616383 | 4.1:-29451775 | None:intergenic |
| ATCGGAGGTTACTGGTATTG+TGG | 0.622916 | 4.1:+29451040 | MS.gene52126:CDS |
| CCTAATCATTCGGTCAGATG+AGG | 0.625575 | 4.1:-29450829 | None:intergenic |
| CCTGTAACAAGCTCTCCTGT+GGG | 0.625764 | 4.1:-29451719 | None:intergenic |
| AACCCTACTTTGAAAAGCGG+TGG | 0.631203 | 4.1:+29450939 | MS.gene52126:CDS |
| GACACTAGATCATGAATCGG+AGG | 0.635591 | 4.1:+29451025 | MS.gene52126:CDS |
| GCAGTTATAAAGCTACCCAC+AGG | 0.640686 | 4.1:+29451704 | MS.gene52126:CDS |
| CCGAACCCTACTTTGAAAAG+CGG | 0.648212 | 4.1:+29450936 | MS.gene52126:CDS |
| AATCCATGATATACATCGGA+CGG | 0.656836 | 4.1:-29450910 | None:intergenic |
| GTGGAATCCATGATATACAT+CGG | 0.668837 | 4.1:-29450914 | None:intergenic |
| GAGACATGGGCGGCTCACGA+GGG | 0.670094 | 4.1:+29451671 | MS.gene52126:CDS |
| CGCAAACTTCATCATCATCG+TGG | 0.680166 | 4.1:-29450770 | None:intergenic |
| AAACTTCATCATCATCGTGG+CGG | 0.709204 | 4.1:-29450767 | None:intergenic |
| GTTTGCGGAATAGCCATCTG+CGG | 0.710293 | 4.1:+29450786 | MS.gene52126:CDS |
| GAATTGCACGGAGACATGGG+CGG | 0.723851 | 4.1:+29451661 | MS.gene52126:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTATAGAGATTAATGAAT+TGG | + | chr4.1:29451373-29451392 | MS.gene52126:intron | 20.0% |
| !! | ATTTATTTGTGATAAAGTGT+TGG | + | chr4.1:29451187-29451206 | MS.gene52126:intron | 20.0% |
| !!! | TTTATTTGTGATAAAGTGTT+GGG | + | chr4.1:29451188-29451207 | MS.gene52126:intron | 20.0% |
| ! | TTGTTTCATCTTCTATTGAT+GGG | + | chr4.1:29451078-29451097 | MS.gene52126:CDS | 25.0% |
| !!! | TACAGGTATTGATACATTTT+CGG | + | chr4.1:29451736-29451755 | MS.gene52126:CDS | 25.0% |
| AATTACTGCACAAGAAATTC+AGG | - | chr4.1:29451613-29451632 | None:intergenic | 30.0% | |
| ATGATACACTTCTGAATGTT+AGG | + | chr4.1:29451533-29451552 | MS.gene52126:intron | 30.0% | |
| CTTGTTTCATCTTCTATTGA+TGG | + | chr4.1:29451077-29451096 | MS.gene52126:CDS | 30.0% | |
| GTTGTGTAGATTCTTTAGTA+AGG | + | chr4.1:29451294-29451313 | MS.gene52126:intron | 30.0% | |
| TACACTTATTATTCTCGTGT+TGG | - | chr4.1:29451224-29451243 | None:intergenic | 30.0% | |
| ! | AACTTTTCATTACCACTTGT+TGG | + | chr4.1:29451763-29451782 | MS.gene52126:CDS | 30.0% |
| ! | ACTTTTCATTACCACTTGTT+GGG | + | chr4.1:29451764-29451783 | MS.gene52126:CDS | 30.0% |
| ! | AGCTTTATAACTGCTTGAAT+TGG | - | chr4.1:29451698-29451717 | None:intergenic | 30.0% |
| ! | TATCAAAAGATGTTTTCGAC+AGG | - | chr4.1:29450878-29450897 | None:intergenic | 30.0% |
| AATCCATGATATACATCGGA+CGG | - | chr4.1:29450913-29450932 | None:intergenic | 35.0% | |
| AGTCAAATTACCAGAGACAT+AGG | + | chr4.1:29451338-29451357 | MS.gene52126:intron | 35.0% | |
| ATTTGAAGAGGTGGAGTAAT+TGG | + | chr4.1:29451630-29451649 | MS.gene52126:CDS | 35.0% | |
| GAAGACACTAGATCATGAAT+CGG | + | chr4.1:29451022-29451041 | MS.gene52126:CDS | 35.0% | |
| GAATAATGCTGTGGTTGTTT+GGG | + | chr4.1:29450977-29450996 | MS.gene52126:CDS | 35.0% | |
| GTGGAATCCATGATATACAT+CGG | - | chr4.1:29450917-29450936 | None:intergenic | 35.0% | |
| TAATAAGTGTAGCTGAGATG+AGG | + | chr4.1:29451233-29451252 | MS.gene52126:intron | 35.0% | |
| TCTTGTGCAGTAATTTGAAG+AGG | + | chr4.1:29451618-29451637 | MS.gene52126:intron | 35.0% | |
| TGAATAATGCTGTGGTTGTT+TGG | + | chr4.1:29450976-29450995 | MS.gene52126:CDS | 35.0% | |
| TTAAAGTCTTCCTATGTCTC+TGG | - | chr4.1:29451351-29451370 | None:intergenic | 35.0% | |
| TTACTGGTATTGTGGTTGAT+GGG | + | chr4.1:29451048-29451067 | MS.gene52126:CDS | 35.0% | |
| TTGGAATTGTTCGAATTGCA+CGG | + | chr4.1:29451649-29451668 | MS.gene52126:CDS | 35.0% | |
| ! | TCATCTTCTATTGATGGGTA+GGG | + | chr4.1:29451083-29451102 | MS.gene52126:intron | 35.0% |
| ! | TTCATCTTCTATTGATGGGT+AGG | + | chr4.1:29451082-29451101 | MS.gene52126:intron | 35.0% |
| !! | ATTTGTGATAAAGTGTTGGG+TGG | + | chr4.1:29451191-29451210 | MS.gene52126:intron | 35.0% |
| AAACTTCATCATCATCGTGG+CGG | - | chr4.1:29450770-29450789 | None:intergenic | 40.0% | |
| CTGTCCAAAGCCTAATCATT+CGG | - | chr4.1:29450842-29450861 | None:intergenic | 40.0% | |
| GTTACTGGTATTGTGGTTGA+TGG | + | chr4.1:29451047-29451066 | MS.gene52126:CDS | 40.0% | |
| TGGTTGTGTGAATAATGCTG+TGG | + | chr4.1:29450968-29450987 | MS.gene52126:CDS | 40.0% | |
| TGTGCAGTAATTTGAAGAGG+TGG | + | chr4.1:29451621-29451640 | MS.gene52126:intron | 40.0% | |
| ! | AAGCTTTATCCCACGAATCA+GGG | - | chr4.1:29451452-29451471 | None:intergenic | 40.0% |
| ! | AGATGAGGTGACTATTTTGC+CGG | - | chr4.1:29450817-29450836 | None:intergenic | 40.0% |
| ! | CACGATGATGATGAAGTTTG+CGG | + | chr4.1:29450771-29450790 | MS.gene52126:CDS | 40.0% |
| ! | TACTGGTATTGTGGTTGATG+GGG | + | chr4.1:29451049-29451068 | MS.gene52126:CDS | 40.0% |
| !! | ATTAGGCTTTGGACAGTTCA+CGG | + | chr4.1:29450846-29450865 | MS.gene52126:CDS | 40.0% |
| AAGGAGAGCAGATCAGATGA+AGG | + | chr4.1:29451313-29451332 | MS.gene52126:intron | 45.0% | |
| ATCGGAGGTTACTGGTATTG+TGG | + | chr4.1:29451040-29451059 | MS.gene52126:CDS | 45.0% | |
| CCTAATCATTCGGTCAGATG+AGG | - | chr4.1:29450832-29450851 | None:intergenic | 45.0% | |
| CCTCATCTGACCGAATGATT+AGG | + | chr4.1:29450829-29450848 | MS.gene52126:CDS | 45.0% | |
| CGCAAACTTCATCATCATCG+TGG | - | chr4.1:29450773-29450792 | None:intergenic | 45.0% | |
| CTGACCGAATGATTAGGCTT+TGG | + | chr4.1:29450835-29450854 | MS.gene52126:CDS | 45.0% | |
| GACACTAGATCATGAATCGG+AGG | + | chr4.1:29451025-29451044 | MS.gene52126:CDS | 45.0% | |
| GATCATGAATCGGAGGTTAC+TGG | + | chr4.1:29451032-29451051 | MS.gene52126:CDS | 45.0% | |
| GCAGTTATAAAGCTACCCAC+AGG | + | chr4.1:29451704-29451723 | MS.gene52126:CDS | 45.0% | |
| GGTCGGCTACATGGATAAAA+CGG | - | chr4.1:29451431-29451450 | None:intergenic | 45.0% | |
| GTTGTGCTGGATCTTGGTTT+GGG | + | chr4.1:29451258-29451277 | MS.gene52126:intron | 45.0% | |
| TCTGAGTGTAACCCAACAAG+TGG | - | chr4.1:29451778-29451797 | None:intergenic | 45.0% | |
| TGTTGTGCTGGATCTTGGTT+TGG | + | chr4.1:29451257-29451276 | MS.gene52126:intron | 45.0% | |
| TTCGAATTGCACGGAGACAT+GGG | + | chr4.1:29451658-29451677 | MS.gene52126:CDS | 45.0% | |
| TTGAAAAGCGGTGGTGTTTC+TGG | + | chr4.1:29450948-29450967 | MS.gene52126:CDS | 45.0% | |
| TTTATCCCACGAATCAGGGT+CGG | - | chr4.1:29451448-29451467 | None:intergenic | 45.0% | |
| ! | AACCCTACTTTGAAAAGCGG+TGG | + | chr4.1:29450939-29450958 | MS.gene52126:CDS | 45.0% |
| ! | CAAGCTTTATCCCACGAATC+AGG | - | chr4.1:29451453-29451472 | None:intergenic | 45.0% |
| ! | CCGAACCCTACTTTGAAAAG+CGG | + | chr4.1:29450936-29450955 | MS.gene52126:CDS | 45.0% |
| ! | CTGATTCGTGGGATAAAGCT+TGG | + | chr4.1:29451451-29451470 | MS.gene52126:intron | 45.0% |
| ! | CTTTTCAAAGTAGGGTTCGG+TGG | - | chr4.1:29450936-29450955 | None:intergenic | 45.0% |
| !! | ACACCACCGCTTTTCAAAGT+AGG | - | chr4.1:29450945-29450964 | None:intergenic | 45.0% |
| !! | CACCACCGCTTTTCAAAGTA+GGG | - | chr4.1:29450944-29450963 | None:intergenic | 45.0% |
| !! | CCGCTTTTCAAAGTAGGGTT+CGG | - | chr4.1:29450939-29450958 | None:intergenic | 45.0% |
| ACCTGTAACAAGCTCTCCTG+TGG | - | chr4.1:29451723-29451742 | None:intergenic | 50.0% | |
| CCTGTAACAAGCTCTCCTGT+GGG | - | chr4.1:29451722-29451741 | None:intergenic | 50.0% | |
| CTGAGATGAGGATGTTGTGC+TGG | + | chr4.1:29451245-29451264 | MS.gene52126:intron | 50.0% | |
| GAGGATGTTGTGCTGGATCT+TGG | + | chr4.1:29451252-29451271 | MS.gene52126:intron | 50.0% | |
| GCACCGTCCGATGTATATCA+TGG | + | chr4.1:29450907-29450926 | MS.gene52126:CDS | 50.0% | |
| GTTCGAATTGCACGGAGACA+TGG | + | chr4.1:29451657-29451676 | MS.gene52126:CDS | 50.0% | |
| GTTTGCGGAATAGCCATCTG+CGG | + | chr4.1:29450786-29450805 | MS.gene52126:CDS | 50.0% | |
| TATACATCGGACGGTGCTTG+CGG | - | chr4.1:29450904-29450923 | None:intergenic | 50.0% | |
| ! | GTTGTTTGGGACGTGGATAG+TGG | + | chr4.1:29450990-29451009 | MS.gene52126:CDS | 50.0% |
| ! | TGGTTTGGGCAAGTAGCAAG+AGG | + | chr4.1:29451272-29451291 | MS.gene52126:intron | 50.0% |
| ACGAATCAGGGTCGGCTACA+TGG | - | chr4.1:29451440-29451459 | None:intergenic | 55.0% | |
| ATACATCGGACGGTGCTTGC+GGG | - | chr4.1:29450903-29450922 | None:intergenic | 55.0% | |
| ATAGCCATCTGCGGCAACAC+CGG | + | chr4.1:29450795-29450814 | MS.gene52126:CDS | 55.0% | |
| GAATTGCACGGAGACATGGG+CGG | + | chr4.1:29451661-29451680 | MS.gene52126:CDS | 55.0% | |
| ! | ATGTAGCCGACCCTGATTCG+TGG | + | chr4.1:29451439-29451458 | MS.gene52126:intron | 55.0% |
| ! | TGCTGTGGTTGTTTGGGACG+TGG | + | chr4.1:29450983-29451002 | MS.gene52126:CDS | 55.0% |
| ! | TGTAGCCGACCCTGATTCGT+GGG | + | chr4.1:29451440-29451459 | MS.gene52126:intron | 55.0% |
| !! | CCCACAGGAGAGCTTGTTAC+AGG | + | chr4.1:29451719-29451738 | MS.gene52126:CDS | 55.0% |
| GGCGGAGTGCACAGCGTAAT+TGG | - | chr4.1:29450752-29450771 | None:intergenic | 60.0% | |
| !! | TTTGCCGGTGTTGCCGCAGA+TGG | - | chr4.1:29450802-29450821 | None:intergenic | 60.0% |
| GAGACATGGGCGGCTCACGA+GGG | + | chr4.1:29451671-29451690 | MS.gene52126:CDS | 65.0% | |
| GGAGACATGGGCGGCTCACG+AGG | + | chr4.1:29451670-29451689 | MS.gene52126:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 29450729 | 29451811 | 29450729 | ID=MS.gene52126 |
| chr4.1 | mRNA | 29450729 | 29451811 | 29450729 | ID=MS.gene52126.t1;Parent=MS.gene52126 |
| chr4.1 | exon | 29450729 | 29451099 | 29450729 | ID=MS.gene52126.t1.exon1;Parent=MS.gene52126.t1 |
| chr4.1 | CDS | 29450729 | 29451099 | 29450729 | ID=cds.MS.gene52126.t1;Parent=MS.gene52126.t1 |
| chr4.1 | exon | 29451628 | 29451811 | 29451628 | ID=MS.gene52126.t1.exon2;Parent=MS.gene52126.t1 |
| chr4.1 | CDS | 29451628 | 29451811 | 29451628 | ID=cds.MS.gene52126.t1;Parent=MS.gene52126.t1 |
| Gene Sequence |
| Protein sequence |
