Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058490.t1 | AAL15651.1 | 100 | 48 | 0 | 0 | 1 | 48 | 1 | 48 | 1.20E-08 | 68.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058490.t1 | G9FYZ4 | 100.0 | 48 | 0 | 0 | 1 | 48 | 1 | 48 | 8.3e-09 | 68.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05474 | MS.gene058490 | 0.856013 | 4.28E-62 | -1.69E-46 |
| MS.gene058490 | MS.gene46301 | 0.801101 | 1.06E-48 | -1.69E-46 |
| MS.gene058490 | MS.gene49902 | 0.822835 | 1.91E-53 | -1.69E-46 |
| MS.gene058490 | MS.gene98856 | 0.831974 | 1.23E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058490.t1 | MTR_6g084640 | 90.909 | 44 | 2 | 2 | 1 | 42 | 1 | 44 | 1.50e-16 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGTCTCAATATCAACAAGG+TGG | + | 43215:13731-13750 | MS.gene058490:CDS | 35.0% | |
| CAACTCCATGATGTTGTTGA+TGG | - | 43215:13871-13890 | MS.gene058490:intergenic | 40.0% | |
| GATTGCCATATGGATCAACT+TGG | - | 43215:13805-13824 | MS.gene058490:intergenic | 40.0% | |
| !! | ACTGGTAGGGTTGATGAATA+TGG | + | 43215:13761-13780 | MS.gene058490:CDS | 40.0% |
| AAGGTGGTGATCAAACTGGT+AGG | + | 43215:13747-13766 | MS.gene058490:CDS | 45.0% | |
| AGGTGGTGATCAAACTGGTA+GGG | + | 43215:13748-13767 | MS.gene058490:CDS | 45.0% | |
| ATGGAGTTGACACAGGTGTT+GGG | + | 43215:13882-13901 | MS.gene058490:CDS | 45.0% | |
| CAACAAGGTGGTGATCAAAC+TGG | + | 43215:13743-13762 | MS.gene058490:CDS | 45.0% | |
| CAACATCATGGAGTTGACAC+AGG | + | 43215:13875-13894 | MS.gene058490:CDS | 45.0% | |
| CATATGGATCAACTTGGCCT+TGG | - | 43215:13799-13818 | MS.gene058490:intergenic | 45.0% | |
| CCACTAATGGGATTGCCATA+TGG | - | 43215:13815-13834 | MS.gene058490:intergenic | 45.0% | |
| CCATATGGCAATCCCATTAG+TGG | + | 43215:13812-13831 | MS.gene058490:CDS | 45.0% | |
| GGACACCATCAACAACATCA+TGG | + | 43215:13863-13882 | MS.gene058490:CDS | 45.0% | |
| TATGGCAATCCCATTAGTGG+TGG | + | 43215:13815-13834 | MS.gene058490:CDS | 45.0% | |
| !! | ACAGGTACTGGTACAGGATA+TGG | + | 43215:13911-13930 | MS.gene058490:CDS | 45.0% |
| !! | CAAGGCCAAGTTGATCCATA+TGG | + | 43215:13797-13816 | MS.gene058490:CDS | 45.0% |
| ACAGGATATGGAACACACGC+TGG | + | 43215:13923-13942 | MS.gene058490:CDS | 50.0% | |
| AGTCATCCCACCACCACTAA+TGG | - | 43215:13828-13847 | MS.gene058490:intergenic | 50.0% | |
| ATGGAACACACGCTGGTAGT+GGG | + | 43215:13930-13949 | MS.gene058490:CDS | 50.0% | |
| CATGGAGTTGACACAGGTGT+TGG | + | 43215:13881-13900 | MS.gene058490:CDS | 50.0% | |
| GCAATCCCATTAGTGGTGGT+GGG | + | 43215:13819-13838 | MS.gene058490:CDS | 50.0% | |
| GTCATCCCACCACCACTAAT+GGG | - | 43215:13827-13846 | MS.gene058490:intergenic | 50.0% | |
| TATGGAAACCCCTTGAGCCA+AGG | + | 43215:13779-13798 | MS.gene058490:CDS | 50.0% | |
| TATGGAACACACGCTGGTAG+TGG | + | 43215:13929-13948 | MS.gene058490:CDS | 50.0% | |
| !! | AGCAACACAGGTACTGGTAC+AGG | + | 43215:13905-13924 | MS.gene058490:CDS | 50.0% |
| !! | ATCAACTTGGCCTTGGCTCA+AGG | - | 43215:13792-13811 | MS.gene058490:intergenic | 50.0% |
| !! | ATGACTGGTGCTACTGGTCA+TGG | + | 43215:13842-13861 | MS.gene058490:CDS | 50.0% |
| !! | ATTAGTGGTGGTGGGATGAC+TGG | + | 43215:13827-13846 | MS.gene058490:CDS | 50.0% |
| !! | TCAACTTGGCCTTGGCTCAA+GGG | - | 43215:13791-13810 | MS.gene058490:intergenic | 50.0% |
| GGCAATCCCATTAGTGGTGG+TGG | + | 43215:13818-13837 | MS.gene058490:CDS | 55.0% | |
| GTTGGGAGCAACACAGGTAC+TGG | + | 43215:13899-13918 | MS.gene058490:CDS | 55.0% | |
| ! | TGGAACACACGCTGGTAGTG+GGG | + | 43215:13931-13950 | MS.gene058490:CDS | 55.0% |
| !! | ACAGGTGTTGGGAGCAACAC+AGG | + | 43215:13893-13912 | MS.gene058490:CDS | 55.0% |
| !! | CAACTTGGCCTTGGCTCAAG+GGG | - | 43215:13790-13809 | MS.gene058490:intergenic | 55.0% |
| !! | AACACACGCTGGTAGTGGGG+GGG | + | 43215:13934-13953 | MS.gene058490:CDS | 60.0% |
| !! | GAACACACGCTGGTAGTGGG+GGG | + | 43215:13933-13952 | MS.gene058490:CDS | 60.0% |
| !! | GGAACACACGCTGGTAGTGG+GGG | + | 43215:13932-13951 | MS.gene058490:CDS | 60.0% |
| !! | GGTGGGATGACTGGTGCTAC+TGG | + | 43215:13836-13855 | MS.gene058490:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43215 | gene | 13731 | 13965 | 13731 | ID=MS.gene058490 |
| 43215 | mRNA | 13731 | 13965 | 13731 | ID=MS.gene058490.t1;Parent=MS.gene058490 |
| 43215 | exon | 13731 | 13965 | 13731 | ID=MS.gene058490.t1.exon1;Parent=MS.gene058490.t1 |
| 43215 | CDS | 13731 | 13965 | 13731 | ID=cds.MS.gene058490.t1;Parent=MS.gene058490.t1 |
| Gene Sequence |
| Protein sequence |
