Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058617.t1 | XP_030944198.1 | 54.6 | 108 | 37 | 4 | 18 | 115 | 44 | 149 | 1.40E-16 | 95.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058617.t1 | Q8RWV3 | 52.9 | 102 | 40 | 2 | 20 | 113 | 80 | 181 | 4.0e-17 | 89.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058617.t1 | A0A2I4G5Y8 | 53.6 | 110 | 39 | 4 | 18 | 117 | 78 | 185 | 9.8e-17 | 95.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058617 | MS.gene31735 | 0.806426 | 8.27E-50 | -1.69E-46 |
| MS.gene058617 | MS.gene61388 | 0.814277 | 1.67E-51 | -1.69E-46 |
| MS.gene058617 | MS.gene64383 | 0.816898 | 4.36E-52 | -1.69E-46 |
| MS.gene058617 | MS.gene81982 | 0.804308 | 2.30E-49 | -1.69E-46 |
| MS.gene058617 | MS.gene97534 | 0.816114 | 6.53E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058617.t1 | MTR_5g055170 | 54.808 | 104 | 39 | 2 | 18 | 113 | 23 | 126 | 1.47e-23 | 93.6 |
| MS.gene058617.t1 | MTR_5g055170 | 54.808 | 104 | 39 | 2 | 18 | 113 | 78 | 181 | 1.70e-23 | 93.6 |
| MS.gene058617.t1 | MTR_5g010140 | 54.808 | 104 | 39 | 2 | 18 | 113 | 78 | 181 | 2.48e-23 | 93.2 |
| MS.gene058617.t1 | MTR_5g010140 | 86.667 | 45 | 3 | 1 | 72 | 113 | 30 | 74 | 1.13e-19 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058617.t1 | AT2G26430 | 52.941 | 102 | 40 | 2 | 20 | 113 | 80 | 181 | 8.91e-23 | 90.5 |
| MS.gene058617.t1 | AT2G26430 | 52.941 | 102 | 40 | 2 | 20 | 113 | 25 | 126 | 2.70e-22 | 90.1 |
| MS.gene058617.t1 | AT2G26430 | 52.941 | 102 | 40 | 2 | 20 | 113 | 25 | 126 | 2.70e-22 | 90.1 |
| MS.gene058617.t1 | AT2G26430 | 52.941 | 102 | 40 | 2 | 20 | 113 | 80 | 181 | 3.13e-22 | 90.1 |
Find 31 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGCTTCTTGCCACAATTC+TGG | 0.179715 | 3.1:-2495630 | None:intergenic |
| GAGCTTGGCTCTGGTATATC+AGG | 0.362422 | 3.1:+2495380 | MS.gene058617:CDS |
| TTTCTCTTCTCTGATGAGCT+TGG | 0.407210 | 3.1:+2495365 | MS.gene058617:CDS |
| TTCTCTGATGAGCTTGGCTC+TGG | 0.414289 | 3.1:+2495371 | MS.gene058617:CDS |
| GTATTCAAAGGTTCAAAACT+TGG | 0.443507 | 3.1:+2495302 | MS.gene058617:CDS |
| GTGGCAAGAAGCTTGGAATC+TGG | 0.445777 | 3.1:+2495638 | MS.gene058617:CDS |
| TGCAAGCTGTGTTTGGTTGG+AGG | 0.460178 | 3.1:+2495247 | MS.gene058617:CDS |
| CAGAATTGTGGCAAGAAGCT+TGG | 0.462318 | 3.1:+2495631 | MS.gene058617:CDS |
| CTTGGAATCTGGCTAATGAT+AGG | 0.463722 | 3.1:+2495649 | MS.gene058617:CDS |
| TGCTGCAAGCTGTGTTTGGT+TGG | 0.475859 | 3.1:+2495244 | MS.gene058617:CDS |
| ACAATTCTGGAGGAGTTTCA+AGG | 0.497950 | 3.1:-2495617 | None:intergenic |
| AAGTTGCTGCAAGCTGTGTT+TGG | 0.499811 | 3.1:+2495240 | MS.gene058617:CDS |
| AAGCTGTGTTTGGTTGGAGG+AGG | 0.507703 | 3.1:+2495250 | MS.gene058617:CDS |
| GTGGGAATCTTGCGGAGTTG+CGG | 0.536199 | 3.1:+2495324 | MS.gene058617:CDS |
| TCAAAGGTTCAAAACTTGGT+GGG | 0.548486 | 3.1:+2495306 | MS.gene058617:CDS |
| AATCTTGCGGAGTTGCGGTT+AGG | 0.552883 | 3.1:+2495329 | MS.gene058617:CDS |
| TTCAAAGGTTCAAAACTTGG+TGG | 0.561146 | 3.1:+2495305 | MS.gene058617:CDS |
| GTATTGAGACTTGTATTCAA+AGG | 0.562259 | 3.1:+2495290 | MS.gene058617:CDS |
| TTGAAACTCCTCCAGAATTG+TGG | 0.562666 | 3.1:+2495619 | MS.gene058617:CDS |
| CAATTCTGGAGGAGTTTCAA+GGG | 0.581722 | 3.1:-2495616 | None:intergenic |
| AACTGAACACTAGACTCCAA+TGG | 0.594487 | 3.1:-2495840 | None:intergenic |
| GAGGAGTTTCAAGGGTGGCA+AGG | 0.594569 | 3.1:-2495608 | None:intergenic |
| AAAACTTGGTGGGAATCTTG+CGG | 0.597311 | 3.1:+2495316 | MS.gene058617:CDS |
| AGCTGTGTTTGGTTGGAGGA+GGG | 0.607090 | 3.1:+2495251 | MS.gene058617:CDS |
| TACAAGTCTCAATACTCCAC+GGG | 0.616213 | 3.1:-2495282 | None:intergenic |
| TAAATTGAAATAAACTTGTG+AGG | 0.617426 | 3.1:-2495585 | None:intergenic |
| AATTGCTGATACAGTGCATG+AGG | 0.624108 | 3.1:+2494542 | MS.gene058617:CDS |
| GCTTCTTGCCACAATTCTGG+AGG | 0.625957 | 3.1:-2495627 | None:intergenic |
| TTCTGGAGGAGTTTCAAGGG+TGG | 0.638431 | 3.1:-2495613 | None:intergenic |
| ATACAAGTCTCAATACTCCA+CGG | 0.639370 | 3.1:-2495283 | None:intergenic |
| GAGGAGGGAGAACTTGCCCG+TGG | 0.669081 | 3.1:+2495266 | MS.gene058617:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATTTTTGTTTTATAAAGTT+AGG | + | chr3.1:2494590-2494609 | MS.gene058617:intron | 15.0% |
| !! | AAAACAAAAATCATCTTTGA+AGG | - | chr3.1:2494582-2494601 | None:intergenic | 20.0% |
| !! | GAAATACGTAAATTTGAAAA+TGG | + | chr3.1:2495509-2495528 | MS.gene058617:intron | 20.0% |
| !! | GTGAAATTATTTGATAAAGT+AGG | + | chr3.1:2494834-2494853 | MS.gene058617:intron | 20.0% |
| !! | TAAATTGAAATAAACTTGTG+AGG | - | chr3.1:2495588-2495607 | None:intergenic | 20.0% |
| !!! | AGACATATTTTGAAAGAAAT+GGG | + | chr3.1:2495549-2495568 | MS.gene058617:CDS | 20.0% |
| !!! | TGTGTTCTGTTAAATTTTAT+AGG | + | chr3.1:2495810-2495829 | MS.gene058617:intron | 20.0% |
| ! | AAAGTCTATGAAAGTAGATT+GGG | + | chr3.1:2494963-2494982 | MS.gene058617:intron | 25.0% |
| ! | AACACAAATAGTATGATTCA+GGG | - | chr3.1:2495796-2495815 | None:intergenic | 25.0% |
| ! | CTTTCCTAAACACTTAAAAA+TGG | - | chr3.1:2494937-2494956 | None:intergenic | 25.0% |
| !!! | AAGAAAGCATTTTGATTCTA+AGG | - | chr3.1:2495158-2495177 | None:intergenic | 25.0% |
| !!! | CTTACCATTTTTAAGTGTTT+AGG | + | chr3.1:2494930-2494949 | MS.gene058617:intron | 25.0% |
| !!! | GAGACATATTTTGAAAGAAA+TGG | + | chr3.1:2495548-2495567 | MS.gene058617:CDS | 25.0% |
| AAGTCTATGAAAGTAGATTG+GGG | + | chr3.1:2494964-2494983 | MS.gene058617:intron | 30.0% | |
| CATCTAACCAACAATATTTC+AGG | - | chr3.1:2494791-2494810 | None:intergenic | 30.0% | |
| GAAAGTCTATGAAAGTAGAT+TGG | + | chr3.1:2494962-2494981 | MS.gene058617:intron | 30.0% | |
| GAACACAAATAGTATGATTC+AGG | - | chr3.1:2495797-2495816 | None:intergenic | 30.0% | |
| GTATTCAAAGGTTCAAAACT+TGG | + | chr3.1:2495302-2495321 | MS.gene058617:CDS | 30.0% | |
| GTATTGAGACTTGTATTCAA+AGG | + | chr3.1:2495290-2495309 | MS.gene058617:CDS | 30.0% | |
| TGCTTTAACAAAGAAGAGAT+CGG | + | chr3.1:2495086-2495105 | MS.gene058617:intron | 30.0% | |
| TGTGAGAGTAATGAATCATA+GGG | - | chr3.1:2495754-2495773 | None:intergenic | 30.0% | |
| TGTTAAAGCAATAGCAATTG+TGG | - | chr3.1:2495076-2495095 | None:intergenic | 30.0% | |
| TGTTGAAATTAAGTACAAGC+AGG | + | chr3.1:2494620-2494639 | MS.gene058617:intron | 30.0% | |
| TTAGTAGCCTGAAATATTGT+TGG | + | chr3.1:2494781-2494800 | MS.gene058617:intron | 30.0% | |
| ! | ATGCTGATTGAGTATATTCA+TGG | + | chr3.1:2495696-2495715 | MS.gene058617:intron | 30.0% |
| !! | TTTTATAGGTTTGCTTCCAT+TGG | + | chr3.1:2495824-2495843 | MS.gene058617:intron | 30.0% |
| !!! | AAGTTGGGAGTCTATTTTTA+AGG | - | chr3.1:2494704-2494723 | None:intergenic | 30.0% |
| !!! | AGATCGGTTTTAATTCGATT+TGG | + | chr3.1:2495102-2495121 | MS.gene058617:intron | 30.0% |
| !!! | AGTTGGGAGTCTATTTTTAA+GGG | - | chr3.1:2494703-2494722 | None:intergenic | 30.0% |
| !!! | ATCGGTTTTAATTCGATTTG+GGG | + | chr3.1:2495104-2495123 | MS.gene058617:intron | 30.0% |
| !!! | GATCGGTTTTAATTCGATTT+GGG | + | chr3.1:2495103-2495122 | MS.gene058617:intron | 30.0% |
| ATACAAGTCTCAATACTCCA+CGG | - | chr3.1:2495286-2495305 | None:intergenic | 35.0% | |
| CTGTGAGAGTAATGAATCAT+AGG | - | chr3.1:2495755-2495774 | None:intergenic | 35.0% | |
| GCAAAGATATCAGCAAATGT+TGG | - | chr3.1:2494899-2494918 | None:intergenic | 35.0% | |
| TAGAGCAGAGAAATGATGAA+TGG | - | chr3.1:2495462-2495481 | None:intergenic | 35.0% | |
| TATCAGGTCTTAGTCACTTA+TGG | + | chr3.1:2495396-2495415 | MS.gene058617:intron | 35.0% | |
| TCAAAGGTTCAAAACTTGGT+GGG | + | chr3.1:2495306-2495325 | MS.gene058617:CDS | 35.0% | |
| TCCTATCCTAAAGTTCAAGT+TGG | - | chr3.1:2494720-2494739 | None:intergenic | 35.0% | |
| TTCAAAGGTTCAAAACTTGG+TGG | + | chr3.1:2495305-2495324 | MS.gene058617:CDS | 35.0% | |
| TTCATTATGCACAAGTCACA+AGG | - | chr3.1:2495187-2495206 | None:intergenic | 35.0% | |
| ! | AAAGCCTCAATTTTCACAGT+AGG | + | chr3.1:2495024-2495043 | MS.gene058617:intron | 35.0% |
| ! | CAAGCAGGTTGAAATTATTC+TGG | + | chr3.1:2494635-2494654 | MS.gene058617:intron | 35.0% |
| ! | CCTATCCTAAAGTTCAAGTT+GGG | - | chr3.1:2494719-2494738 | None:intergenic | 35.0% |
| !!! | CTATTTTTAAGGGAGGATGT+TGG | - | chr3.1:2494693-2494712 | None:intergenic | 35.0% |
| AATTGCTGATACAGTGCATG+AGG | + | chr3.1:2494542-2494561 | MS.gene058617:CDS | 40.0% | |
| AGACTCCCAACTTGAACTTT+AGG | + | chr3.1:2494711-2494730 | MS.gene058617:intron | 40.0% | |
| AGCTCCTACTGTGAAAATTG+AGG | - | chr3.1:2495031-2495050 | None:intergenic | 40.0% | |
| ATGATTCAGGGATCGTATAC+AGG | - | chr3.1:2495784-2495803 | None:intergenic | 40.0% | |
| CTTGGAATCTGGCTAATGAT+AGG | + | chr3.1:2495649-2495668 | MS.gene058617:CDS | 40.0% | |
| TACAAGTCTCAATACTCCAC+GGG | - | chr3.1:2495285-2495304 | None:intergenic | 40.0% | |
| TCTATGAAAGTAGATTGGGG+AGG | + | chr3.1:2494967-2494986 | MS.gene058617:intron | 40.0% | |
| TGATTCAGGGATCGTATACA+GGG | - | chr3.1:2495783-2495802 | None:intergenic | 40.0% | |
| TTGAAACTCCTCCAGAATTG+TGG | + | chr3.1:2495619-2495638 | MS.gene058617:CDS | 40.0% | |
| TTTCTCTTCTCTGATGAGCT+TGG | + | chr3.1:2495365-2495384 | MS.gene058617:CDS | 40.0% | |
| ! | AAAACTTGGTGGGAATCTTG+CGG | + | chr3.1:2495316-2495335 | MS.gene058617:CDS | 40.0% |
| ! | ACAATTCTGGAGGAGTTTCA+AGG | - | chr3.1:2495620-2495639 | None:intergenic | 40.0% |
| ! | CAATTCTGGAGGAGTTTCAA+GGG | - | chr3.1:2495619-2495638 | None:intergenic | 40.0% |
| !! | CCCAACTTGAACTTTAGGAT+AGG | + | chr3.1:2494716-2494735 | MS.gene058617:intron | 40.0% |
| !! | TTTTTAAGGGAGGATGTTGG+TGG | - | chr3.1:2494690-2494709 | None:intergenic | 40.0% |
| !!! | TGGGAGTCTATTTTTAAGGG+AGG | - | chr3.1:2494700-2494719 | None:intergenic | 40.0% |
| AAAGTAGATTGGGGAGGAAG+AGG | + | chr3.1:2494973-2494992 | MS.gene058617:intron | 45.0% | |
| AAGTAGATTGGGGAGGAAGA+GGG | + | chr3.1:2494974-2494993 | MS.gene058617:intron | 45.0% | |
| AAGTTGCTGCAAGCTGTGTT+TGG | + | chr3.1:2495240-2495259 | MS.gene058617:CDS | 45.0% | |
| AGGAAGAGGGTTTGACTGAA+GGG | + | chr3.1:2494987-2495006 | MS.gene058617:intron | 45.0% | |
| AGGGTACGTATAGTGTCAAG+TGG | - | chr3.1:2495735-2495754 | None:intergenic | 45.0% | |
| AGGGTTTGACTGAAGGGATT+AGG | + | chr3.1:2494993-2495012 | MS.gene058617:intron | 45.0% | |
| CAAGCTTCTTGCCACAATTC+TGG | - | chr3.1:2495633-2495652 | None:intergenic | 45.0% | |
| CAGAATTGTGGCAAGAAGCT+TGG | + | chr3.1:2495631-2495650 | MS.gene058617:CDS | 45.0% | |
| AAGCTGTGTTTGGTTGGAGG+AGG | + | chr3.1:2495250-2495269 | MS.gene058617:CDS | 50.0% | |
| AATCTTGCGGAGTTGCGGTT+AGG | + | chr3.1:2495329-2495348 | MS.gene058617:CDS | 50.0% | |
| AGCTGTGTTTGGTTGGAGGA+GGG | + | chr3.1:2495251-2495270 | MS.gene058617:CDS | 50.0% | |
| CAGGGATCGTATACAGGGAT+GGG | - | chr3.1:2495778-2495797 | None:intergenic | 50.0% | |
| GAGGAAGAGGGTTTGACTGA+AGG | + | chr3.1:2494986-2495005 | MS.gene058617:intron | 50.0% | |
| GCTTCTTGCCACAATTCTGG+AGG | - | chr3.1:2495630-2495649 | None:intergenic | 50.0% | |
| GTGGCAAGAAGCTTGGAATC+TGG | + | chr3.1:2495638-2495657 | MS.gene058617:CDS | 50.0% | |
| TCAGGGATCGTATACAGGGA+TGG | - | chr3.1:2495779-2495798 | None:intergenic | 50.0% | |
| TGCAAGCTGTGTTTGGTTGG+AGG | + | chr3.1:2495247-2495266 | MS.gene058617:CDS | 50.0% | |
| TGCTGCAAGCTGTGTTTGGT+TGG | + | chr3.1:2495244-2495263 | MS.gene058617:CDS | 50.0% | |
| TTCTCTGATGAGCTTGGCTC+TGG | + | chr3.1:2495371-2495390 | MS.gene058617:CDS | 50.0% | |
| ! | TTCTGGAGGAGTTTCAAGGG+TGG | - | chr3.1:2495616-2495635 | None:intergenic | 50.0% |
| !! | GAGCTTGGCTCTGGTATATC+AGG | + | chr3.1:2495380-2495399 | MS.gene058617:CDS | 50.0% |
| GAGGAGTTTCAAGGGTGGCA+AGG | - | chr3.1:2495611-2495630 | None:intergenic | 55.0% | |
| GTGGGAATCTTGCGGAGTTG+CGG | + | chr3.1:2495324-2495343 | MS.gene058617:CDS | 55.0% | |
| GAGGAGGGAGAACTTGCCCG+TGG | + | chr3.1:2495266-2495285 | MS.gene058617:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 2494510 | 2495853 | 2494510 | ID=MS.gene058617 |
| chr3.1 | mRNA | 2494510 | 2495853 | 2494510 | ID=MS.gene058617.t1;Parent=MS.gene058617 |
| chr3.1 | exon | 2494510 | 2494563 | 2494510 | ID=MS.gene058617.t1.exon1;Parent=MS.gene058617.t1 |
| chr3.1 | CDS | 2494510 | 2494563 | 2494510 | ID=cds.MS.gene058617.t1;Parent=MS.gene058617.t1 |
| chr3.1 | exon | 2495239 | 2495401 | 2495239 | ID=MS.gene058617.t1.exon2;Parent=MS.gene058617.t1 |
| chr3.1 | CDS | 2495239 | 2495401 | 2495239 | ID=cds.MS.gene058617.t1;Parent=MS.gene058617.t1 |
| chr3.1 | exon | 2495547 | 2495670 | 2495547 | ID=MS.gene058617.t1.exon3;Parent=MS.gene058617.t1 |
| chr3.1 | CDS | 2495547 | 2495670 | 2495547 | ID=cds.MS.gene058617.t1;Parent=MS.gene058617.t1 |
| chr3.1 | exon | 2495832 | 2495853 | 2495832 | ID=MS.gene058617.t1.exon4;Parent=MS.gene058617.t1 |
| chr3.1 | CDS | 2495832 | 2495853 | 2495832 | ID=cds.MS.gene058617.t1;Parent=MS.gene058617.t1 |
| Gene Sequence |
| Protein sequence |
