AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97534


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97534.t1 MTR_2g038510 92.222 90 7 0 1 90 1 90 5.27e-52 175
MS.gene97534.t1 MTR_7g015810 46.392 97 43 2 1 88 3 99 1.58e-24 90.1
MS.gene97534.t1 MTR_7g015770 42.982 114 61 2 1 113 3 113 1.39e-23 94.7
MS.gene97534.t1 MTR_5g071310 33.333 93 62 0 1 93 1 93 7.17e-13 63.9
MS.gene97534.t1 MTR_5g070960 28.431 102 73 0 1 102 1 102 1.38e-11 60.5
MS.gene97534.t1 MTR_5g072140 28.431 102 73 0 1 102 1 102 1.44e-11 60.5
MS.gene97534.t1 MTR_5g070470 27.451 102 74 0 1 102 1 102 1.60e-11 60.1
MS.gene97534.t1 MTR_5g072250 28.431 102 73 0 1 102 1 102 2.19e-11 59.7
MS.gene97534.t1 MTR_3g012090 32.979 94 57 2 1 88 1 94 2.27e-11 59.7
MS.gene97534.t1 MTR_5g021080 31.325 83 53 1 1 79 1 83 3.62e-11 59.3
MS.gene97534.t1 MTR_1g022380 30.588 85 59 0 1 85 1 85 3.74e-11 59.3
MS.gene97534.t1 MTR_7g071940 30.108 93 65 0 1 93 1 93 3.85e-11 59.3
MS.gene97534.t1 MTR_5g072340 27.451 102 74 0 1 102 1 102 7.51e-11 58.2
MS.gene97534.t1 MTR_5g071670 29.032 93 66 0 1 93 1 93 9.19e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97534.t1 AT1G50180 40.708 113 65 1 1 111 1 113 2.54e-19 82.4
MS.gene97534.t1 AT1G58390 39.759 83 50 0 1 83 1 83 4.93e-15 70.5
MS.gene97534.t1 AT1G58390 39.759 83 50 0 1 83 1 83 4.93e-15 70.5
MS.gene97534.t1 AT3G46730 41.463 82 48 0 1 82 1 82 6.50e-15 70.1
MS.gene97534.t1 AT1G58400 33.333 87 58 0 1 87 1 87 2.97e-14 68.2
MS.gene97534.t1 AT1G58400 33.333 87 58 0 1 87 1 87 3.30e-14 67.8
MS.gene97534.t1 AT1G58400 33.333 87 58 0 1 87 1 87 3.30e-14 67.8
MS.gene97534.t1 AT1G58807 37.349 83 52 0 1 83 1 83 5.39e-14 67.4
MS.gene97534.t1 AT1G59124 37.349 83 52 0 1 83 1 83 5.39e-14 67.4
MS.gene97534.t1 AT1G58807 37.349 83 52 0 1 83 1 83 6.08e-14 67.4
MS.gene97534.t1 AT1G59124 37.349 83 52 0 1 83 1 83 6.08e-14 67.4
MS.gene97534.t1 AT1G58848 37.349 83 52 0 1 83 1 83 7.32e-14 67.0
MS.gene97534.t1 AT1G59218 37.349 83 52 0 1 83 1 83 7.32e-14 67.0
MS.gene97534.t1 AT1G59218 37.349 83 52 0 1 83 1 83 7.32e-14 67.0
MS.gene97534.t1 AT1G59218 37.349 83 52 0 1 83 1 83 7.32e-14 67.0
MS.gene97534.t1 AT1G58848 37.349 83 52 0 1 83 1 83 7.32e-14 67.0
MS.gene97534.t1 AT1G53350 36.145 83 53 0 1 83 1 83 1.35e-13 66.2
MS.gene97534.t1 AT1G58602 36.145 83 53 0 1 83 1 83 1.80e-13 65.9
MS.gene97534.t1 AT1G58602 36.145 83 53 0 1 83 1 83 1.80e-13 65.9
MS.gene97534.t1 AT3G46710 39.535 86 52 0 1 86 1 86 4.73e-13 64.7
MS.gene97534.t1 AT3G46530 35.789 95 58 1 1 92 1 95 2.05e-12 62.8
MS.gene97534.t1 AT1G58410 36.709 79 50 0 5 83 3 81 3.58e-12 62.0
MS.gene97534.t1 AT1G58410 36.709 79 50 0 5 83 3 81 3.58e-12 62.0
MS.gene97534.t1 AT1G58410 36.709 79 50 0 5 83 3 81 3.58e-12 62.0
MS.gene97534.t1 AT1G58410 36.709 79 50 0 5 83 3 81 3.71e-12 62.0
MS.gene97534.t1 AT5G35450 33.735 83 55 0 1 83 1 83 2.01e-11 60.1
MS.gene97534.t1 AT5G35450 33.735 83 55 0 1 83 1 83 2.01e-11 60.1
MS.gene97534.t1 AT5G43470 30.682 88 61 0 1 88 1 88 2.15e-11 60.1
MS.gene97534.t1 AT5G43470 30.682 88 61 0 1 88 1 88 2.15e-11 60.1
MS.gene97534.t1 AT5G43470 30.682 88 61 0 1 88 1 88 2.15e-11 60.1
MS.gene97534.t1 AT5G43470 30.682 88 61 0 1 88 1 88 2.15e-11 60.1
MS.gene97534.t1 AT5G48620 31.325 83 57 0 1 83 1 83 4.37e-11 58.9
MS.gene97534.t1 AT5G48620 31.325 83 57 0 1 83 1 83 4.37e-11 58.9
MS.gene97534.t1 AT5G48620 31.325 83 57 0 1 83 1 83 4.37e-11 58.9
MS.gene97534.t1 AT5G48620 31.325 83 57 0 1 83 1 83 4.37e-11 58.9
MS.gene97534.t1 AT5G48620 31.325 83 57 0 1 83 1 83 4.37e-11 58.9
MS.gene97534.t1 AT5G48620 31.325 83 57 0 1 83 1 83 4.37e-11 58.9
MS.gene97534.t1 AT1G59780 35.366 82 53 0 1 82 1 82 5.15e-11 58.9
MS.gene97534.t1 AT1G59780 35.366 82 53 0 1 82 6 87 5.73e-11 58.5

Find 10 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAAGTTGTGTTCTTATA+TGG 0.260674 7.2:-74518639 MS.gene97534:CDS
AAGATGAATCTCTCAAGAAT+TGG 0.362537 7.2:-74518533 MS.gene97534:CDS
ACCTGTCATGTTTCTTCTTG+AGG 0.429591 7.2:+74518443 None:intergenic
TGTACTGTGAAGTCTATCAT+TGG 0.440500 7.2:+74518684 None:intergenic
TCAAGTGGAGAAGAAGTGTT+TGG 0.444195 7.2:-74518021 MS.gene97534:CDS
TGTGAAGTCTATCATTGGTT+TGG 0.462179 7.2:+74518689 None:intergenic
AAAGTCTTAGAACTGAATTA+AGG 0.463863 7.2:-74518599 MS.gene97534:CDS
AAATCTGTATCAAGTTCAAG+TGG 0.573359 7.2:-74518036 MS.gene97534:CDS
GCCTCAAGAAGAAACATGAC+AGG 0.606493 7.2:-74518444 MS.gene97534:intron
ACTTGTGAGTATGAAAGACG+AGG 0.697547 7.2:-74518070 MS.gene97534:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAAAATGATGTTAATATTT+TGG - chr7.2:74518382-74518401 MS.gene97534:intron 15.0%
!! TATATCATAGTACTAAAGTT+AGG - chr7.2:74518498-74518517 MS.gene97534:CDS 20.0%
!! TTGGTTAATAACAACAATTA+GGG - chr7.2:74518413-74518432 MS.gene97534:intron 20.0%
!! TTTGGTTAATAACAACAATT+AGG - chr7.2:74518412-74518431 MS.gene97534:intron 20.0%
!!! AAAAGATTTGTTTCAATACA+TGG - chr7.2:74518299-74518318 MS.gene97534:intron 20.0%
!!! AAAGATTTGTTTCAATACAT+GGG - chr7.2:74518300-74518319 MS.gene97534:intron 20.0%
!!! AAATCTTTTGATTCTGTTTA+AGG + chr7.2:74518288-74518307 None:intergenic 20.0%
! AAAGTCTTAGAACTGAATTA+AGG - chr7.2:74518105-74518124 MS.gene97534:intron 25.0%
!!! ATGCAAAATTGTTGCTATTT+GGG - chr7.2:74518436-74518455 MS.gene97534:intron 25.0%
!!! TAATATTTTGGAGTCATGTT+TGG - chr7.2:74518394-74518413 MS.gene97534:intron 25.0%
AAATCTGTATCAAGTTCAAG+TGG - chr7.2:74518668-74518687 MS.gene97534:CDS 30.0%
AAGATGAATCTCTCAAGAAT+TGG - chr7.2:74518171-74518190 MS.gene97534:intron 30.0%
AGGTTTAGGAAAAACAACAT+TGG - chr7.2:74518466-74518485 MS.gene97534:CDS 30.0%
GATGAAGTTGTGTTCTTATA+TGG - chr7.2:74518065-74518084 MS.gene97534:CDS 30.0%
TACTCTCATGTTATTGAAGA+GGG - chr7.2:74518347-74518366 MS.gene97534:intron 30.0%
TCTTCTCTTAACTCTTTAGA+AGG + chr7.2:74518614-74518633 None:intergenic 30.0%
TTACTCTCATGTTATTGAAG+AGG - chr7.2:74518346-74518365 MS.gene97534:intron 30.0%
! GATGCAAAATTGTTGCTATT+TGG - chr7.2:74518435-74518454 MS.gene97534:intron 30.0%
! GTTATTGAAGAGGGTATAAT+TGG - chr7.2:74518356-74518375 MS.gene97534:intron 30.0%
!! AAAATTTTGAGAGCTTAGCT+TGG - chr7.2:74518522-74518541 MS.gene97534:CDS 30.0%
!! AAATTTTGAGAGCTTAGCTT+GGG - chr7.2:74518523-74518542 MS.gene97534:CDS 30.0%
!! TGCAAAATTGTTGCTATTTG+GGG - chr7.2:74518437-74518456 MS.gene97534:intron 30.0%
ATCACAACATTGTCAAGCAA+GGG - chr7.2:74518553-74518572 MS.gene97534:CDS 35.0%
TATCACAACATTGTCAAGCA+AGG - chr7.2:74518552-74518571 MS.gene97534:CDS 35.0%
TGTACTGTGAAGTCTATCAT+TGG + chr7.2:74518023-74518042 None:intergenic 35.0%
! TGTGAAGTCTATCATTGGTT+TGG + chr7.2:74518018-74518037 None:intergenic 35.0%
!! AATTGTTGCTATTTGGGGTA+TGG - chr7.2:74518442-74518461 MS.gene97534:intron 35.0%
!! ATTGTTGCTATTTGGGGTAT+GGG - chr7.2:74518443-74518462 MS.gene97534:intron 35.0%
ACCTGTCATGTTTCTTCTTG+AGG + chr7.2:74518264-74518283 None:intergenic 40.0%
ACTTGTGAGTATGAAAGACG+AGG - chr7.2:74518634-74518653 MS.gene97534:CDS 40.0%
ATTGTCAAGCAAGGGATGTT+TGG - chr7.2:74518561-74518580 MS.gene97534:CDS 40.0%
TTGTCAAGCAAGGGATGTTT+GGG - chr7.2:74518562-74518581 MS.gene97534:CDS 40.0%
!! TCAAGTGGAGAAGAAGTGTT+TGG - chr7.2:74518683-74518702 MS.gene97534:CDS 40.0%
AAGCAAGGGATGTTTGGGAA+GGG - chr7.2:74518567-74518586 MS.gene97534:CDS 45.0%
GCCTCAAGAAGAAACATGAC+AGG - chr7.2:74518260-74518279 MS.gene97534:intron 45.0%
! ATTTGGGGTATGGGAGGTTT+AGG - chr7.2:74518452-74518471 MS.gene97534:CDS 45.0%
CAAGCAAGGGATGTTTGGGA+AGG - chr7.2:74518566-74518585 MS.gene97534:CDS 50.0%
!! GTTGCTATTTGGGGTATGGG+AGG - chr7.2:74518446-74518465 MS.gene97534:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 74518011 74518715 74518011 ID=MS.gene97534
chr7.2 mRNA 74518011 74518715 74518011 ID=MS.gene97534.t1;Parent=MS.gene97534
chr7.2 exon 74518445 74518715 74518445 ID=MS.gene97534.t1.exon1;Parent=MS.gene97534.t1
chr7.2 CDS 74518445 74518715 74518445 ID=cds.MS.gene97534.t1;Parent=MS.gene97534.t1
chr7.2 exon 74518011 74518105 74518011 ID=MS.gene97534.t1.exon2;Parent=MS.gene97534.t1
chr7.2 CDS 74518011 74518105 74518011 ID=cds.MS.gene97534.t1;Parent=MS.gene97534.t1
Gene Sequence

>MS.gene97534

ATGGCCAAACCAATGATAGACTTCACAGTACAAAAAATCAGTGATTTACTCATTGATGAAGTTGTGTTCTTATATGGAGTGAAAGACAAAGTGCAAAGTCTTAGAACTGAATTAAGGATGATAGAATCTTACTTACAAGATGCAGATAGAAAGCAAGATGAAGATGAATCTCTCAAGAATTGGATTTCAGAAATCAGAGAAGTTGCTTATGATTCAGATGATGTCATTGAAGCATATGCACTCAAAGAAGCCTCAAGAAGAAACATGACAGGTACCTTAAACAGAATCAAAAGATTTGTTTCAATACATGGGAGAAACAAAGCTTTGAGAAGATCTTACTCTCATGTTATTGAAGAGGGTATAATTGGTGTTGAAAATGATGTTAATATTTTGGAGTCATGTTTGGTTAATAACAACAATTAGGGATGCAAAATTGTTGCTATTTGGGGTATGGGAGGTTTAGGAAAAACAACATTGGCTAAGAAAGTATATCATAGTACTAAAGTTAGGCAAAATTTTGAGAGCTTAGCTTGGGCTTATATATCACAACATTGTCAAGCAAGGGATGTTTGGGAAGGGATTTTGTTGAAGCTTTTATCTCCTTCTAAAGAGTTAAGAGAAGAACTTGTGAGTATGAAAGACGAGGAAGTTGCAAAAAAATCTGTATCAAGTTCAAGTGGAGAAGAAGTGTTTGGTAGTTCTTGA

Protein sequence

>MS.gene97534.t1

MAKPMIDFTVQKISDLLIDEVVFLYGVKDKVQSLRTELRMIESYLQDADRKQDEDESLKNWISEIREVAYDSDDVIEAYALKEASRRNMTELREELVSMKDEEVAKKSVSSSSGEEVFGSS