AlfalfaGEDB | MS.gene31735

M. sativa cultivar XinJiangDaYe / MS.gene31735

KEGG: K03138
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005634 GO:0006139 GO:0006352 GO:0006355 GO:0006357 GO:0006367 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0034243 GO:0034641 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0110165 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF05793.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31735.t1	KAA0066238.1	67.1	76	14	1	1	76	194	258	3.20E-15	90.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31735.t1	A0A5A7VEV3	67.1	76	14	1	1	76	194	258	2.3e-15	90.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050026	MS.gene31735	0.806316	8.73E-50	-1.69E-46
MS.gene051181	MS.gene31735	0.807134	5.86E-50	-1.69E-46
MS.gene05125	MS.gene31735	0.808557	2.92E-50	-1.69E-46
MS.gene053621	MS.gene31735	0.806386	8.43E-50	-1.69E-46
MS.gene053998	MS.gene31735	0.804127	2.51E-49	-1.69E-46
MS.gene05415	MS.gene31735	0.809239	2.09E-50	-1.69E-46
MS.gene05417	MS.gene31735	0.81358	2.38E-51	-1.69E-46
MS.gene054236	MS.gene31735	0.822045	2.92E-53	-1.69E-46
MS.gene055818	MS.gene31735	0.810176	1.31E-50	-1.69E-46
MS.gene056050	MS.gene31735	0.810549	1.09E-50	-1.69E-46
MS.gene056142	MS.gene31735	0.810322	1.22E-50	-1.69E-46
MS.gene056173	MS.gene31735	0.80174	7.81E-49	-1.69E-46
MS.gene056174	MS.gene31735	0.803669	3.12E-49	-1.69E-46
MS.gene058010	MS.gene31735	0.81484	1.26E-51	-1.69E-46
MS.gene058515	MS.gene31735	0.820238	7.62E-53	-1.69E-46
MS.gene058617	MS.gene31735	0.806426	8.27E-50	-1.69E-46
MS.gene058979	MS.gene31735	0.8134	2.61E-51	-1.69E-46
MS.gene05925	MS.gene31735	0.823976	1.04E-53	-1.69E-46
MS.gene059272	MS.gene31735	0.805844	1.10E-49	-1.69E-46
MS.gene059584	MS.gene31735	0.806192	9.27E-50	-1.69E-46
MS.gene060011	MS.gene31735	0.822039	2.93E-53	-1.69E-46
MS.gene060167	MS.gene31735	0.812578	3.95E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31735.t1	MTR_2g025620	48.855	131	10	2	1	74	182	312	1.03e-26	101
MS.gene31735.t1	MTR_2g025590	51.181	127	9	3	1	74	192	318	2.11e-26	100

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31735.t1	AT4G12610	41.667	132	19	6	1	74	192	323	1.43e-15	70.5
MS.gene31735.t1	AT4G12610	41.667	132	19	6	1	74	192	323	2.09e-15	69.7

Find 25 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTAACCTGCTTGATTTC+AGG	0.208605	1.1:+13125632	None:intergenic
AAAGGAGCTGTTTACTTAAC+AGG	0.231443	1.1:-13125266	MS.gene31735:CDS
TTTCGAGGAAAGGGAAGATT+TGG	0.304420	1.1:-13125675	MS.gene31735:CDS
TCAGGAGGAGCGGGGACTTC+AGG	0.323204	1.1:+13125650	None:intergenic
ATCTGTTACAGGTGATGATT+GGG	0.426973	1.1:-13125744	MS.gene31735:intron
AACTCTTGGCAATGATTTCG+AGG	0.465620	1.1:-13125690	MS.gene31735:CDS
TGCTTGATTTCAGGAGGAGC+GGG	0.471514	1.1:+13125641	None:intergenic
AATAATGGTCCTGCTGCATT+TGG	0.478973	1.1:-13126451	MS.gene31735:CDS
TGGCAATGATTTCGAGGAAA+GGG	0.482007	1.1:-13125684	MS.gene31735:CDS
TATCTGTTACAGGTGATGAT+TGG	0.485168	1.1:-13125745	MS.gene31735:intron
CCTGCTGCATTTGGTGAACA+TGG	0.497830	1.1:-13126442	MS.gene31735:CDS
ACTGATGATGATGAAACTCT+TGG	0.499952	1.1:-13125704	MS.gene31735:CDS
ATGATGAAAGTAGCAAATAA+TGG	0.508669	1.1:-13126466	MS.gene31735:CDS
CGCTCCTCCTGAAATCAAGC+AGG	0.530063	1.1:-13125639	MS.gene31735:intron
CTTGATAACTTGCAGAACAA+AGG	0.543909	1.1:-13125284	MS.gene31735:intron
CCATGTTCACCAAATGCAGC+AGG	0.556267	1.1:+13126442	None:intergenic
TTGGCAATGATTTCGAGGAA+AGG	0.576029	1.1:-13125685	MS.gene31735:CDS
CTGCTTGATTTCAGGAGGAG+CGG	0.591513	1.1:+13125640	None:intergenic
AAGGAGCTGTTTACTTAACA+GGG	0.603006	1.1:-13125265	MS.gene31735:CDS
TGATGATTGGGAGCATGAAG+AGG	0.605131	1.1:-13125732	MS.gene31735:CDS
ACATGGGAAGTTAGATGACA+AGG	0.613551	1.1:-13126425	MS.gene31735:CDS
CTAACCTGCTTGATTTCAGG+AGG	0.619687	1.1:+13125635	None:intergenic
GCTTGATTTCAGGAGGAGCG+GGG	0.624057	1.1:+13125642	None:intergenic
AAAACTGGTGATGATGATGA+AGG	0.653364	1.1:-13126382	MS.gene31735:intron
CTGCTGCATTTGGTGAACAT+GGG	0.666508	1.1:-13126441	MS.gene31735:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CATAAAATATAAAACAAACA+AGG	+	chr1.1:13125521-13125540	None:intergenic	15.0%
!!	TTTATGGAAATATGTATATT+TGG	-	chr1.1:13126257-13126276	MS.gene31735:intron	15.0%
!!	ATATATTAGGTAATTAGGTA+TGG	-	chr1.1:13126229-13126248	MS.gene31735:intron	20.0%
!!	ATTAGGTATGGATTTATTTA+TGG	-	chr1.1:13126241-13126260	MS.gene31735:intron	20.0%
!!	TACATTATAAATCTGCTAAT+AGG	-	chr1.1:13125608-13125627	MS.gene31735:intron	20.0%
!!	TAGCAGATTTATAATGTATA+TGG	+	chr1.1:13125606-13125625	None:intergenic	20.0%
!!	TCTACAATTGGTTATATATT+AGG	-	chr1.1:13126216-13126235	MS.gene31735:intron	20.0%
!!	TGGTTATATATTAGGTAATT+AGG	-	chr1.1:13126224-13126243	MS.gene31735:intron	20.0%
!	AATTCTTATTGAAATCAAGC+AGG	-	chr1.1:13126114-13126133	MS.gene31735:intron	25.0%
!	ATCATGCAAATAAACTAGTT+AGG	+	chr1.1:13126367-13126386	None:intergenic	25.0%
!	ATGATGAAAGTAGCAAATAA+TGG	-	chr1.1:13125247-13125266	MS.gene31735:CDS	25.0%
!	GAAACTAACCAAAAGTAAAT+TGG	+	chr1.1:13125562-13125581	None:intergenic	25.0%
!	GATGATTATGATGTTGAAAA+GGG	-	chr1.1:13125484-13125503	MS.gene31735:intron	25.0%
!	TGATGATTATGATGTTGAAA+AGG	-	chr1.1:13125483-13125502	MS.gene31735:intron	25.0%
!!	ATTTTATGAATCCATGTTGA+AGG	-	chr1.1:13125533-13125552	MS.gene31735:intron	25.0%
	CAAGTTATCAAGATAATCCA+GGG	+	chr1.1:13126421-13126440	None:intergenic	30.0%
	CATCATAATCATCATCATCA+AGG	+	chr1.1:13125477-13125496	None:intergenic	30.0%
	CTCACAAGTATAAGAATTTG+TGG	+	chr1.1:13126152-13126171	None:intergenic	30.0%
	GTCATAGCTAATTCTACAAT+TGG	-	chr1.1:13126204-13126223	MS.gene31735:intron	30.0%
	TGCATGATTTCAATGAAGTT+CGG	-	chr1.1:13126379-13126398	MS.gene31735:intron	30.0%
	TGTACTTGTATATCTGTTAC+AGG	-	chr1.1:13125958-13125977	MS.gene31735:intron	30.0%
	TTCAATAAGAATTTGTGCAC+TGG	+	chr1.1:13126107-13126126	None:intergenic	30.0%
	TTGTAGAATTAGCTATGACA+TGG	+	chr1.1:13126203-13126222	None:intergenic	30.0%
!	CATGGACTGAATAAAAAAAG+TGG	-	chr1.1:13125412-13125431	MS.gene31735:intron	30.0%
!	GAAGGTTACCAATTTACTTT+TGG	-	chr1.1:13125551-13125570	MS.gene31735:intron	30.0%
!	TAAATTGGTAACCTTCAACA+TGG	+	chr1.1:13125547-13125566	None:intergenic	30.0%
!!!	CATCATCACCAGTTTTTTTA+CGG	+	chr1.1:13125327-13125346	None:intergenic	30.0%
	AAACTAACCTGCTTGATTTC+AGG	+	chr1.1:13126084-13126103	None:intergenic	35.0%
	AAAGGAGCTGTTTACTTAAC+AGG	-	chr1.1:13126447-13126466	MS.gene31735:CDS	35.0%
	AAGAGAAGCCGTAAAAAAAC+TGG	-	chr1.1:13125316-13125335	MS.gene31735:intron	35.0%
	AAGGAGCTGTTTACTTAACA+GGG	-	chr1.1:13126448-13126467	MS.gene31735:CDS	35.0%
	AGAGAATCAACGTTATAGTC+AGG	+	chr1.1:13125801-13125820	None:intergenic	35.0%
	ATCTGTTACAGGTGATGATT+GGG	-	chr1.1:13125969-13125988	MS.gene31735:intron	35.0%
	GCAAGTTATCAAGATAATCC+AGG	+	chr1.1:13126422-13126441	None:intergenic	35.0%
	TATCTGTTACAGGTGATGAT+TGG	-	chr1.1:13125968-13125987	MS.gene31735:intron	35.0%
	TTCAATGAAGTTCGGATAGA+GGG	-	chr1.1:13126387-13126406	MS.gene31735:CDS	35.0%
	TTTCAATGAAGTTCGGATAG+AGG	-	chr1.1:13126386-13126405	MS.gene31735:CDS	35.0%
!	AAAACTGGTGATGATGATGA+AGG	-	chr1.1:13125331-13125350	MS.gene31735:intron	35.0%
!	ACTGATGATGATGAAACTCT+TGG	-	chr1.1:13126009-13126028	MS.gene31735:intron	35.0%
!	ACTTTTATCCTCCAAAAGGA+GGG	-	chr1.1:13126280-13126299	MS.gene31735:intron	35.0%
!	CTTGATAACTTGCAGAACAA+AGG	-	chr1.1:13126429-13126448	MS.gene31735:CDS	35.0%
!	TATGATGATGACGATGATGA+AGG	-	chr1.1:13125436-13125455	MS.gene31735:intron	35.0%
!	TTGGACTTTTATCCTCCAAA+AGG	-	chr1.1:13126276-13126295	MS.gene31735:intron	35.0%
	AACTCTTGGCAATGATTTCG+AGG	-	chr1.1:13126023-13126042	MS.gene31735:intron	40.0%
	AATAATGGTCCTGCTGCATT+TGG	-	chr1.1:13125262-13125281	MS.gene31735:CDS	40.0%
	ACATGGGAAGTTAGATGACA+AGG	-	chr1.1:13125288-13125307	MS.gene31735:intron	40.0%
	ACTTGAGTAGAGCTTGGATA+AGG	-	chr1.1:13125713-13125732	MS.gene31735:CDS	40.0%
	ATCTTCCTCTCCTTTATCAG+AGG	+	chr1.1:13125362-13125381	None:intergenic	40.0%
	ATGAAGACGAGGAAGTAGAT+AGG	-	chr1.1:13125380-13125399	MS.gene31735:intron	40.0%
	CTTAGATGCCATAACTCACA+CGG	+	chr1.1:13125744-13125763	None:intergenic	40.0%
	GTAGATAGGAAGAGTAGACA+TGG	-	chr1.1:13125394-13125413	MS.gene31735:intron	40.0%
	TGGCAATGATTTCGAGGAAA+GGG	-	chr1.1:13126029-13126048	MS.gene31735:intron	40.0%
	TTAGATGCCATAACTCACAC+GGG	+	chr1.1:13125743-13125762	None:intergenic	40.0%
	TTGGCAATGATTTCGAGGAA+AGG	-	chr1.1:13126028-13126047	MS.gene31735:intron	40.0%
	TTTCGAGGAAAGGGAAGATT+TGG	-	chr1.1:13126038-13126057	MS.gene31735:intron	40.0%
!	GACTTTTATCCTCCAAAAGG+AGG	-	chr1.1:13126279-13126298	MS.gene31735:intron	40.0%
	AGGATAACCCGTGTGAGTTA+TGG	-	chr1.1:13125733-13125752	MS.gene31735:CDS	45.0%
	ATGACGATGATGAAGGTCCA+AGG	-	chr1.1:13125443-13125462	MS.gene31735:intron	45.0%
	CTAACCTGCTTGATTTCAGG+AGG	+	chr1.1:13126081-13126100	None:intergenic	45.0%
	GAAGGTCCTTCCTCTGATAA+AGG	-	chr1.1:13125349-13125368	MS.gene31735:intron	45.0%
	TCCTCTCCTTTATCAGAGGA+AGG	+	chr1.1:13125358-13125377	None:intergenic	45.0%
	TCCTTCCTCTGATAAAGGAG+AGG	-	chr1.1:13125354-13125373	MS.gene31735:intron	45.0%
	TGACGATGATGAAGGTCCAA+GGG	-	chr1.1:13125444-13125463	MS.gene31735:intron	45.0%
!	CATCTAGTCAACCCTCCTTT+TGG	+	chr1.1:13126294-13126313	None:intergenic	45.0%
!	CTGCTGCATTTGGTGAACAT+GGG	-	chr1.1:13125272-13125291	MS.gene31735:CDS	45.0%
!	TGATGATTGGGAGCATGAAG+AGG	-	chr1.1:13125981-13126000	MS.gene31735:intron	45.0%
	AGGAGAGGAAGATGAAGACG+AGG	-	chr1.1:13125369-13125388	MS.gene31735:intron	50.0%
	ATGATGAAGGTCCAAGGGGA+GGG	-	chr1.1:13125449-13125468	MS.gene31735:intron	50.0%
	CCATGTTCACCAAATGCAGC+AGG	+	chr1.1:13125274-13125293	None:intergenic	50.0%
	CGCAAGACTTGAGTAGAGCT+TGG	-	chr1.1:13125707-13125726	MS.gene31735:CDS	50.0%
	CTGCTTGATTTCAGGAGGAG+CGG	+	chr1.1:13126076-13126095	None:intergenic	50.0%
	GACGATGATGAAGGTCCAAG+GGG	-	chr1.1:13125445-13125464	MS.gene31735:intron	50.0%
	TGCTTGATTTCAGGAGGAGC+GGG	+	chr1.1:13126075-13126094	None:intergenic	50.0%
!	CCTGCTGCATTTGGTGAACA+TGG	-	chr1.1:13125271-13125290	MS.gene31735:CDS	50.0%
!	CTAGTCAACCCTCCTTTTGG+AGG	+	chr1.1:13126291-13126310	None:intergenic	50.0%
	CGCTCCTCCTGAAATCAAGC+AGG	-	chr1.1:13126074-13126093	MS.gene31735:intron	55.0%
	GATAGAGGGACTAGAGACCC+TGG	-	chr1.1:13126401-13126420	MS.gene31735:CDS	55.0%
	GATGATGAAGGTCCAAGGGG+AGG	-	chr1.1:13125448-13125467	MS.gene31735:intron	55.0%
	GCTTGATTTCAGGAGGAGCG+GGG	+	chr1.1:13126074-13126093	None:intergenic	55.0%
	TGATGAAGGTCCAAGGGGAG+GGG	-	chr1.1:13125450-13125469	MS.gene31735:intron	55.0%
	TCATCAAGGTCCCCTCCCCT+TGG	+	chr1.1:13125463-13125482	None:intergenic	60.0%
	TCAGGAGGAGCGGGGACTTC+AGG	+	chr1.1:13126066-13126085	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	13125247	13126488	13125247	ID=MS.gene31735
chr1.1	mRNA	13125247	13126488	13125247	ID=MS.gene31735.t1;Parent=MS.gene31735
chr1.1	exon	13126383	13126488	13126383	ID=MS.gene31735.t1.exon1;Parent=MS.gene31735.t1
chr1.1	CDS	13126383	13126488	13126383	ID=cds.MS.gene31735.t1;Parent=MS.gene31735.t1
chr1.1	exon	13125640	13125755	13125640	ID=MS.gene31735.t1.exon2;Parent=MS.gene31735.t1
chr1.1	CDS	13125640	13125755	13125640	ID=cds.MS.gene31735.t1;Parent=MS.gene31735.t1
chr1.1	exon	13125247	13125291	13125247	ID=MS.gene31735.t1.exon3;Parent=MS.gene31735.t1
chr1.1	CDS	13125247	13125291	13125247	ID=cds.MS.gene31735.t1;Parent=MS.gene31735.t1

Gene Sequence

>MS.gene31735

ATGATGAAAGTAGCAAATAATGGTCCTGCTGCATTTGGTGAACATGGGAAGTTAGATGACAAGGAAAGCAAGAGAAGCCGTAAAAAAACTGGTGATGATGATGAAGGTCCTTCCTCTGATAAAGGAGAGGAAGATGAAGACGAGGAAGTAGATAGGAAGAGTAGACATGGACTGAATAAAAAAAGTGGCTATGATGATGACGATGATGAAGGTCCAAGGGGAGGGGACCTTGATGATGATGATTATGATGTTGAAAAGGGTGAGTATCTTGCCTTGTTTGTTTTATATTTTATGAATCCATGTTGAAGGTTACCAATTTACTTTTGGTTAGTTTCATGTAGTGAACAATTTTTTTACCATATACATTATAAATCTGCTAATAGGATAATCTGAGAACATATTGTTTAAAAGTTGTCTAACACAAAAATCTGTTAATTAGTAAGTAAGAGACTGATAAGCTCGCAAGACTTGAGTAGAGCTTGGATAAGGATAACCCGTGTGAGTTATGGCATCTAAGTACAATGTGCTGATTTTGTAGCACAACTAATTAGCCTGACTATAACGTTGATTCTCTCTCTCTCTCTCTCTCTATATATATATATATATATATATATATAGAGAGAGAGAGAGAGAGAGAGAGAGAGTTAACTTGATATAAATTGAAAACTTTCAGATCACAGAGTTTGTATTTATTCTTTCTGTAAGTATAGTTGTACTTGTATATCTGTTACAGGTGATGATTGGGAGCATGAAGAGGTTTTCACTGATGATGATGAAACTCTTGGCAATGATTTCGAGGAAAGGGAAGATTTGGATCCTGAAGTCCCCGCTCCTCCTGAAATCAAGCAGGTTAGTTTCCAGTGCACAAATTCTTATTGAAATCAAGCAGGTTAGTTTCTAATCCACAAATTCTTATACTTGTGAGTGTGACATGTCAATCATAATTGCTCTTTCCATGTCATAGCTAATTCTACAATTGGTTATATATTAGGTAATTAGGTATGGATTTATTTATGGAAATATGTATATTTGGACTTTTATCCTCCAAAAGGAGGGTTGACTAGATGAAAGATAATTTAATAGTAAAAGATAGACGAAAGATAATTTAGATAGCGCGCCTAACTAGTTTATTTGCATGATTTCAATGAAGTTCGGATAGAGGGACTAGAGACCCTGGATTATCTTGATAACTTGCAGAACAAAGGAGCTGTTTACTTAACAGGGAGATGCTTTAACTTTTGA

Protein sequence

>MS.gene31735.t1

MMKVANNGPAAFGEHGKLDDKESKRSRKKTGDDDEGDDWEHEEVFTDDDETLGNDFEEREDLDPEVPAPPEIKQNKGAVYLTGRCFNF