AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64383

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64383.t1 MTR_2g086200 57.627 118 12 1 17 134 118 197 1.95e-37 131
MS.gene64383.t1 MTR_2g086200 54.082 98 16 2 17 112 118 188 6.40e-23 94.7
MS.gene64383.t1 MTR_2g086200 84.615 39 6 0 245 283 185 223 1.72e-14 71.6
MS.gene64383.t1 MTR_0057s0200 82.979 47 8 0 236 282 1 47 5.40e-21 84.3
MS.gene64383.t1 MTR_5g077900 80.392 51 10 0 232 282 164 214 9.38e-21 88.6
MS.gene64383.t1 MTR_0002s1270 80.392 51 10 0 232 282 338 388 5.35e-20 89.4
MS.gene64383.t1 MTR_7g046380 78.431 51 11 0 232 282 346 396 1.01e-19 88.6
MS.gene64383.t1 MTR_4g066320 76.000 50 11 1 234 282 60 109 4.53e-18 78.6
MS.gene64383.t1 MTR_8g057120 43.820 89 48 2 145 233 25 111 4.95e-14 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 57 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCTTCCTTGCTAAGTTT+TGG 0.180064 2.4:-35630659 None:intergenic
CTTGTATTTCTTGTCGGAAC+TGG 0.206895 2.4:+35630633 MS.gene64383:CDS
CATTGGACACAATACTTTCT+TGG 0.226158 2.4:-35631053 None:intergenic
AACTTAGCAAGGAAGAAATT+AGG 0.231126 2.4:+35630663 MS.gene64383:CDS
CTACATTGCCTTCCTCTCTT+TGG 0.237868 2.4:-35630766 None:intergenic
TACATTGCCTTCCTCTCTTT+GGG 0.257496 2.4:-35630765 None:intergenic
ACTTAGCAAGGAAGAAATTA+GGG 0.297782 2.4:+35630664 MS.gene64383:CDS
AAAATTATACCCAATGGATA+TGG 0.330708 2.4:+35630714 MS.gene64383:CDS
TTGGCATCGTAATGACGATT+TGG 0.337903 2.4:-35631034 None:intergenic
GTTAAGATTGTTTAATTTGA+AGG 0.342364 2.4:+35630931 MS.gene64383:CDS
CATGTCCTTGTATTTCTTGT+CGG 0.358491 2.4:+35630627 MS.gene64383:CDS
GTATGCTTTGCGAAACGATT+AGG 0.383236 2.4:+35631430 MS.gene64383:CDS
TATCCACAATCAGTTGTGTT+AGG 0.390652 2.4:-35631209 None:intergenic
TTAAGTCGTGAGTATGATAA+TGG 0.392342 2.4:+35630545 MS.gene64383:CDS
CGGGAAAATTTCGTGCATCC+TGG 0.394745 2.4:-35630896 None:intergenic
AGAGGAAGGCAATGTAGATA+TGG 0.403013 2.4:+35630772 MS.gene64383:CDS
TTGTTGACAGAAATGCTTCC+AGG 0.403895 2.4:+35630998 MS.gene64383:CDS
AAAAGTTTCACTGAATTGCT+TGG 0.418634 2.4:+35630974 MS.gene64383:CDS
GAAGGCATGATACGTGATAT+TGG 0.421913 2.4:+35630806 MS.gene64383:CDS
TTGTATTTCTTGTCGGAACT+GGG 0.448957 2.4:+35630634 MS.gene64383:CDS
CTGCTAATATTACTACCTCT+TGG 0.458064 2.4:+35631264 MS.gene64383:CDS
TTTCACCATGCCATATCCAT+TGG 0.463974 2.4:-35630724 None:intergenic
AAATCCCTTTCGAAACTAGA+AGG 0.466148 2.4:-35630688 None:intergenic
TCCTCTCTTTGGGATACACT+TGG 0.470015 2.4:-35630755 None:intergenic
TTTGGAATTGTGTTACCTCC+TGG 0.480826 2.4:-35631016 None:intergenic
TGATTGGGTAGATAGCAAAA+AGG 0.489439 2.4:+35630092 MS.gene64383:CDS
GATATGGATGATAGACTTGA+AGG 0.489740 2.4:+35630788 MS.gene64383:CDS
TTCACCATGCCATATCCATT+GGG 0.498165 2.4:-35630723 None:intergenic
GGGAAAATTTCGTGCATCCT+GGG 0.498909 2.4:-35630895 None:intergenic
ATTCCTATACCTACGAATCA+TGG 0.502884 2.4:-35629072 None:intergenic
ATCTTAACACATCTGACAGC+CGG 0.502928 2.4:-35630916 None:intergenic
CAACCTAACACAACTGATTG+TGG 0.514100 2.4:+35631206 MS.gene64383:CDS
TGATAATTTATGTAAAGATA+AGG 0.516510 2.4:+35630859 MS.gene64383:CDS
TTTGTCAAAATTATACCCAA+TGG 0.528540 2.4:+35630708 MS.gene64383:CDS
TATTACTACCTCTTGGATGA+AGG 0.535487 2.4:+35631271 MS.gene64383:CDS
GGGGCCTTCTAGTTTCGAAA+GGG 0.542428 2.4:+35630684 MS.gene64383:CDS
AGGGGCCTTCTAGTTTCGAA+AGG 0.561989 2.4:+35630683 MS.gene64383:CDS
CGACAAGAAATACAAGGACA+TGG 0.566912 2.4:-35630626 None:intergenic
TCTTAACACATCTGACAGCC+GGG 0.570003 2.4:-35630915 None:intergenic
GCTATCTACCCAATCAGCAA+TGG 0.571793 2.4:-35630085 None:intergenic
TTCGTGCATCCTGGGTACAA+AGG 0.580916 2.4:-35630887 None:intergenic
TCATTGTCTCAAAATTTCAT+CGG 0.587000 2.4:+35629032 MS.gene64383:CDS
CAGTTCCGACAAGAAATACA+AGG 0.588437 2.4:-35630632 None:intergenic
AGTGTATCCCAAAGAGAGGA+AGG 0.595288 2.4:+35630758 MS.gene64383:CDS
ACGTAGTCATGGATCGTAGG+TGG 0.603715 2.4:+35630507 MS.gene64383:CDS
CTGGGACCCAAAACTTAGCA+AGG 0.616572 2.4:+35630652 MS.gene64383:CDS
AGAACGTAGTCATGGATCGT+AGG 0.617787 2.4:+35630504 MS.gene64383:intron
TACCTACGAATCATGGACAC+TGG 0.625314 2.4:-35629065 None:intergenic
TATACCCAATGGATATGGCA+TGG 0.625444 2.4:+35630719 MS.gene64383:CDS
GCCAAGTGTATCCCAAAGAG+AGG 0.637721 2.4:+35630754 MS.gene64383:CDS
TGCCAGTGTCCATGATTCGT+AGG 0.646406 2.4:+35629063 MS.gene64383:CDS
AAGAATCTTCCTAACACCGA+GGG 0.647769 2.4:+35630596 MS.gene64383:CDS
AAGGAACAACCTTTGTACCC+AGG 0.660647 2.4:+35630878 MS.gene64383:CDS
ATGGTATACCTTCATCCAAG+AGG 0.683941 2.4:-35631279 None:intergenic
CTTAGCAAGGAAGAAATTAG+GGG 0.692233 2.4:+35630665 MS.gene64383:CDS
TTGACAGAAATGCTTCCAGG+AGG 0.719496 2.4:+35631001 MS.gene64383:CDS
AAAGAATCTTCCTAACACCG+AGG 0.743980 2.4:+35630595 MS.gene64383:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAGTAAAATAACAATA+AGG - chr2.4:35631350-35631369 None:intergenic 10.0%
!! ACATACTAAATTAAAAAATT+GGG - chr2.4:35630474-35630493 None:intergenic 10.0%
!! TAAAAATTAAATTCATTTGT+AGG + chr2.4:35631175-35631194 MS.gene64383:intron 10.0%
!!! TAATTTAGTATTATTTTTGT+AGG - chr2.4:35629428-35629447 None:intergenic 10.0%
! GGGGGGGGGGGGGGGGGGGG+GGG + chr2.4:35629179-35629198 MS.gene64383:intron 100.0%
!! ATATCACAATTTAAATTTGA+GGG + chr2.4:35629679-35629698 MS.gene64383:intron 15.0%
!! CACATACTAAATTAAAAAAT+TGG - chr2.4:35630475-35630494 None:intergenic 15.0%
!! TATATCACAATTTAAATTTG+AGG + chr2.4:35629678-35629697 MS.gene64383:intron 15.0%
!! TGATAATTTATGTAAAGATA+AGG + chr2.4:35630859-35630878 MS.gene64383:CDS 15.0%
!!! CATTTTATTTCAATATTCTT+GGG + chr2.4:35629780-35629799 MS.gene64383:intron 15.0%
!!! TCTTAATTTGGAAATATTTA+TGG - chr2.4:35631314-35631333 None:intergenic 15.0%
!! CCAAGAATATTGAAATAAAA+TGG - chr2.4:35629782-35629801 None:intergenic 20.0%
!! TATTGCTTATAGGAAAAATA+TGG + chr2.4:35629893-35629912 MS.gene64383:intron 20.0%
!!! CCATTTTATTTCAATATTCT+TGG + chr2.4:35629779-35629798 MS.gene64383:intron 20.0%
!!! GTTAAGATTGTTTAATTTGA+AGG + chr2.4:35630931-35630950 MS.gene64383:CDS 20.0%
!!! TTTAATCTGATTATGTGTTT+TGG + chr2.4:35629298-35629317 MS.gene64383:intron 20.0%
!!! TTTAATTTGAAGGCAAAAAA+TGG + chr2.4:35630941-35630960 MS.gene64383:CDS 20.0%
! AAAATTATACCCAATGGATA+TGG + chr2.4:35630714-35630733 MS.gene64383:CDS 25.0%
! AAATGATGATTAAATGAGGT+GGG + chr2.4:35629323-35629342 MS.gene64383:intron 25.0%
! AAATTGTAAAGGACTAAGAT+GGG - chr2.4:35629384-35629403 None:intergenic 25.0%
! ATATTTATGGTGCAAACATA+TGG - chr2.4:35631301-35631320 None:intergenic 25.0%
! ATTAAATTCATTTGTAGGCT+AGG + chr2.4:35631180-35631199 MS.gene64383:intron 25.0%
! CATAATCAGATTAAAAAGAG+TGG - chr2.4:35629293-35629312 None:intergenic 25.0%
! GGTTAAATGATGATTAAATG+AGG + chr2.4:35629319-35629338 MS.gene64383:intron 25.0%
! TAAAATGTAAGGAATGTGTA+AGG - chr2.4:35629266-35629285 None:intergenic 25.0%
! TAAATGATGATTAAATGAGG+TGG + chr2.4:35629322-35629341 MS.gene64383:intron 25.0%
! TAATCTATTGTACAAACAGT+AGG + chr2.4:35631370-35631389 MS.gene64383:intron 25.0%
! TATGTCTAGCTATCTTAATT+TGG - chr2.4:35631326-35631345 None:intergenic 25.0%
! TCATTGTCTCAAAATTTCAT+CGG + chr2.4:35629032-35629051 MS.gene64383:CDS 25.0%
! TCCCCTATTTGAAAATATTA+GGG - chr2.4:35629453-35629472 None:intergenic 25.0%
! TTATTTCAATATTCTTGGGT+AGG + chr2.4:35629784-35629803 MS.gene64383:intron 25.0%
! TTTGTCAAAATTATACCCAA+TGG + chr2.4:35630708-35630727 MS.gene64383:CDS 25.0%
!! AGTCCCTAATATTTTCAAAT+AGG + chr2.4:35629447-35629466 MS.gene64383:intron 25.0%
!! GTCCCTAATATTTTCAAATA+GGG + chr2.4:35629448-35629467 MS.gene64383:intron 25.0%
!! GTTTTTGTCATATACTTATC+TGG + chr2.4:35630269-35630288 MS.gene64383:intron 25.0%
!! TACACATTCCTTACATTTTA+TGG + chr2.4:35629266-35629285 MS.gene64383:intron 25.0%
!! TCCCTAATATTTTCAAATAG+GGG + chr2.4:35629449-35629468 MS.gene64383:intron 25.0%
!! TTTTTCCTATAAGCAATACA+AGG - chr2.4:35629891-35629910 None:intergenic 25.0%
AAAAGTTTCACTGAATTGCT+TGG + chr2.4:35630974-35630993 MS.gene64383:CDS 30.0%
AAACAACATGATGCACATAA+AGG - chr2.4:35630253-35630272 None:intergenic 30.0%
AACATGTTGGAGACTAATTT+AGG + chr2.4:35629524-35629543 MS.gene64383:intron 30.0%
AACTTAGCAAGGAAGAAATT+AGG + chr2.4:35630663-35630682 MS.gene64383:CDS 30.0%
ACTTAGCAAGGAAGAAATTA+GGG + chr2.4:35630664-35630683 MS.gene64383:CDS 30.0%
AGCTGATCTCAAAAAAATTG+AGG + chr2.4:35630125-35630144 MS.gene64383:CDS 30.0%
AGTCCTTTACAATTTCTTTG+TGG + chr2.4:35629389-35629408 MS.gene64383:intron 30.0%
ATGTTATCTTGTTGAGAATC+AGG - chr2.4:35630161-35630180 None:intergenic 30.0%
ATTTGAAGGCAAAAAATGGA+TGG + chr2.4:35630945-35630964 MS.gene64383:CDS 30.0%
CAAGAAGGACAATGATAATT+CGG - chr2.4:35629834-35629853 None:intergenic 30.0%
GAAATTGTAAAGGACTAAGA+TGG - chr2.4:35629385-35629404 None:intergenic 30.0%
GTCCCCTATTTGAAAATATT+AGG - chr2.4:35629454-35629473 None:intergenic 30.0%
TGAACTCTCAATCTAAACTT+TGG + chr2.4:35630020-35630039 MS.gene64383:intron 30.0%
TTAAGTCGTGAGTATGATAA+TGG + chr2.4:35630545-35630564 MS.gene64383:CDS 30.0%
! ATTATATGTTGTCGCTTGTT+GGG + chr2.4:35629604-35629623 MS.gene64383:intron 30.0%
! ATTTCTTCCTTGCTAAGTTT+TGG - chr2.4:35630662-35630681 None:intergenic 30.0%
! TAATTTTCAGAACGTAGTCA+TGG + chr2.4:35630496-35630515 MS.gene64383:intron 30.0%
! TATTATATGTTGTCGCTTGT+TGG + chr2.4:35629603-35629622 MS.gene64383:intron 30.0%
! TTTCTTCCTTGCTAAGTTTT+GGG - chr2.4:35630661-35630680 None:intergenic 30.0%
!! AAGAAAGTATTGTGTCCAAT+GGG + chr2.4:35631055-35631074 MS.gene64383:intron 30.0%
!!! ATTTTATGTTGTTGCTTTGC+TGG + chr2.4:35629144-35629163 MS.gene64383:intron 30.0%
!!! TTCTTTGTTTTCATCATGCA+CGG - chr2.4:35630403-35630422 None:intergenic 30.0%
!!! TTTTATGTTGTTGCTTTGCT+GGG + chr2.4:35629145-35629164 MS.gene64383:intron 30.0%
AAATCCCTTTCGAAACTAGA+AGG - chr2.4:35630691-35630710 None:intergenic 35.0%
AAATCGTGGAAGTCGAAATT+GGG + chr2.4:35629569-35629588 MS.gene64383:intron 35.0%
AGAGTGGTCCATAAAATGTA+AGG - chr2.4:35629277-35629296 None:intergenic 35.0%
ATGATTCGTAGGTATAGGAA+TGG + chr2.4:35629074-35629093 MS.gene64383:intron 35.0%
ATTCCTATACCTACGAATCA+TGG - chr2.4:35629075-35629094 None:intergenic 35.0%
CAATGATTCAGAGACTAAAC+TGG + chr2.4:35629491-35629510 MS.gene64383:intron 35.0%
CAATTTAAATTTGAGGGTCC+TGG + chr2.4:35629685-35629704 MS.gene64383:intron 35.0%
CATGTCCTTGTATTTCTTGT+CGG + chr2.4:35630627-35630646 MS.gene64383:CDS 35.0%
CATTGGACACAATACTTTCT+TGG - chr2.4:35631056-35631075 None:intergenic 35.0%
CGAATTATCATTGTCCTTCT+TGG + chr2.4:35629832-35629851 MS.gene64383:intron 35.0%
CTGCTAATATTACTACCTCT+TGG + chr2.4:35631264-35631283 MS.gene64383:CDS 35.0%
CTGTAGAATGAAAGCTTAGT+AGG + chr2.4:35630445-35630464 MS.gene64383:intron 35.0%
CTTAGCAAGGAAGAAATTAG+GGG + chr2.4:35630665-35630684 MS.gene64383:CDS 35.0%
GAGCCACAAAGAAATTGTAA+AGG - chr2.4:35629395-35629414 None:intergenic 35.0%
GATATGGATGATAGACTTGA+AGG + chr2.4:35630788-35630807 MS.gene64383:CDS 35.0%
TATCATTGTCCTTCTTGGAT+TGG + chr2.4:35629837-35629856 MS.gene64383:intron 35.0%
TATCCACAATCAGTTGTGTT+AGG - chr2.4:35631212-35631231 None:intergenic 35.0%
TATTACTACCTCTTGGATGA+AGG + chr2.4:35631271-35631290 MS.gene64383:CDS 35.0%
TGATTGGGTAGATAGCAAAA+AGG + chr2.4:35630092-35630111 MS.gene64383:CDS 35.0%
TGTCATATACTTATCTGGAG+TGG + chr2.4:35630274-35630293 MS.gene64383:intron 35.0%
TTGTATTTCTTGTCGGAACT+GGG + chr2.4:35630634-35630653 MS.gene64383:CDS 35.0%
! ACATCTTTTCCATTGCTGAT+TGG + chr2.4:35630076-35630095 MS.gene64383:CDS 35.0%
! TTTGCTGGGAGTATGAAATT+GGG + chr2.4:35629159-35629178 MS.gene64383:intron 35.0%
! TTTTCATACTCCATACCCAT+TGG - chr2.4:35631073-35631092 None:intergenic 35.0%
!! CAAGAAAGTATTGTGTCCAA+TGG + chr2.4:35631054-35631073 MS.gene64383:intron 35.0%
!! CATCTTTTCCATTGCTGATT+GGG + chr2.4:35630077-35630096 MS.gene64383:CDS 35.0%
AAAGAATCTTCCTAACACCG+AGG + chr2.4:35630595-35630614 MS.gene64383:CDS 40.0%
AAGAATCTTCCTAACACCGA+GGG + chr2.4:35630596-35630615 MS.gene64383:CDS 40.0%
AATACAAGGCGACCCAATTA+CGG - chr2.4:35629877-35629896 None:intergenic 40.0%
AATTTAGGCCCTTGTGAGTT+GGG + chr2.4:35629539-35629558 MS.gene64383:intron 40.0%
ACACAACAACAGAGAACCTA+TGG + chr2.4:35630376-35630395 MS.gene64383:intron 40.0%
AGAGGAAGGCAATGTAGATA+TGG + chr2.4:35630772-35630791 MS.gene64383:CDS 40.0%
AGGTTCTCTGTTGTTGTGTT+TGG - chr2.4:35630375-35630394 None:intergenic 40.0%
AGTATTGTGTCCAATGGGTA+TGG + chr2.4:35631060-35631079 MS.gene64383:intron 40.0%
AGTCTCCAACATGTTCATAG+TGG - chr2.4:35629519-35629538 None:intergenic 40.0%
ATCTTAACACATCTGACAGC+CGG - chr2.4:35630919-35630938 None:intergenic 40.0%
ATGGTATACCTTCATCCAAG+AGG - chr2.4:35631282-35631301 None:intergenic 40.0%
ATTCGTAGGTATAGGAATGG+TGG + chr2.4:35629077-35629096 MS.gene64383:intron 40.0%
CAACCTAACACAACTGATTG+TGG + chr2.4:35631206-35631225 MS.gene64383:CDS 40.0%
CAGTTCCGACAAGAAATACA+AGG - chr2.4:35630635-35630654 None:intergenic 40.0%
CGACAAGAAATACAAGGACA+TGG - chr2.4:35630629-35630648 None:intergenic 40.0%
CTTGTATTTCTTGTCGGAAC+TGG + chr2.4:35630633-35630652 MS.gene64383:CDS 40.0%
GAAATCGTGGAAGTCGAAAT+TGG + chr2.4:35629568-35629587 MS.gene64383:intron 40.0%
GTGTAGTGTTGTCCGTAATT+GGG + chr2.4:35629862-35629881 MS.gene64383:intron 40.0%
TAACCATCTTGTCTACCCAT+TGG - chr2.4:35631115-35631134 None:intergenic 40.0%
TAATTTAGGCCCTTGTGAGT+TGG + chr2.4:35629538-35629557 MS.gene64383:intron 40.0%
TACATTGCCTTCCTCTCTTT+GGG - chr2.4:35630768-35630787 None:intergenic 40.0%
TAGGTATAGGAATGGTGGTA+GGG + chr2.4:35629082-35629101 MS.gene64383:intron 40.0%
TATACCCAATGGATATGGCA+TGG + chr2.4:35630719-35630738 MS.gene64383:CDS 40.0%
TGGATCCACTATGAACATGT+TGG + chr2.4:35629511-35629530 MS.gene64383:intron 40.0%
TGTCCATGATTCGTAGGTAT+AGG + chr2.4:35629069-35629088 MS.gene64383:intron 40.0%
TTCACCATGCCATATCCATT+GGG - chr2.4:35630726-35630745 None:intergenic 40.0%
TTGGCATCGTAATGACGATT+TGG - chr2.4:35631037-35631056 None:intergenic 40.0%
TTGTTGACAGAAATGCTTCC+AGG + chr2.4:35630998-35631017 MS.gene64383:CDS 40.0%
TTTCACCATGCCATATCCAT+TGG - chr2.4:35630727-35630746 None:intergenic 40.0%
! CTTTGCTGGGAGTATGAAAT+TGG + chr2.4:35629158-35629177 MS.gene64383:intron 40.0%
! GAAGGCATGATACGTGATAT+TGG + chr2.4:35630806-35630825 MS.gene64383:CDS 40.0%
! TTGCTGGGAGTATGAAATTG+GGG + chr2.4:35629160-35629179 MS.gene64383:intron 40.0%
! TTTGGAATTGTGTTACCTCC+TGG - chr2.4:35631019-35631038 None:intergenic 40.0%
AAGGAACAACCTTTGTACCC+AGG + chr2.4:35630878-35630897 MS.gene64383:CDS 45.0%
ACATGCTAGACATCGTCCAA+TGG + chr2.4:35631096-35631115 MS.gene64383:intron 45.0%
ACTACACCACCAATCCAAGA+AGG - chr2.4:35629849-35629868 None:intergenic 45.0%
AGAACGTAGTCATGGATCGT+AGG + chr2.4:35630504-35630523 MS.gene64383:intron 45.0%
AGGACATGGAAAAACTCCCT+CGG - chr2.4:35630615-35630634 None:intergenic 45.0%
AGTCCTGCATTAGAAGATCC+AGG - chr2.4:35629706-35629725 None:intergenic 45.0%
AGTGTATCCCAAAGAGAGGA+AGG + chr2.4:35630758-35630777 MS.gene64383:CDS 45.0%
ATGGTGGTAGGGTCACAATT+TGG + chr2.4:35629093-35629112 MS.gene64383:intron 45.0%
CATGCTAGACATCGTCCAAT+GGG + chr2.4:35631097-35631116 MS.gene64383:intron 45.0%
CTACATTGCCTTCCTCTCTT+TGG - chr2.4:35630769-35630788 None:intergenic 45.0%
GCTATCTACCCAATCAGCAA+TGG - chr2.4:35630088-35630107 None:intergenic 45.0%
GGGAAAATTTCGTGCATCCT+GGG - chr2.4:35630898-35630917 None:intergenic 45.0%
GGTAGGCTATCTTTACCTCA+CGG + chr2.4:35629801-35629820 MS.gene64383:intron 45.0%
GGTCGCCTTGTATTGCTTAT+AGG + chr2.4:35629883-35629902 MS.gene64383:intron 45.0%
GGTGTAGTGTTGTCCGTAAT+TGG + chr2.4:35629861-35629880 MS.gene64383:intron 45.0%
GTAGGTATAGGAATGGTGGT+AGG + chr2.4:35629081-35629100 MS.gene64383:intron 45.0%
TACCTACGAATCATGGACAC+TGG - chr2.4:35629068-35629087 None:intergenic 45.0%
TCTTAACACATCTGACAGCC+GGG - chr2.4:35630918-35630937 None:intergenic 45.0%
TGGTGGTAGGGTCACAATTT+GGG + chr2.4:35629094-35629113 MS.gene64383:intron 45.0%
TTGACAGAAATGCTTCCAGG+AGG + chr2.4:35631001-35631020 MS.gene64383:CDS 45.0%
TTTCATCATGCACGGTCCAT+AGG - chr2.4:35630395-35630414 None:intergenic 45.0%
! ACGGTGTTGGGACTTGAAAT+TGG + chr2.4:35629640-35629659 MS.gene64383:intron 45.0%
! CAGGATGCACGAAATTTTCC+CGG + chr2.4:35630897-35630916 MS.gene64383:CDS 45.0%
! CATTGTCCTTCTTGGATTGG+TGG + chr2.4:35629840-35629859 MS.gene64383:intron 45.0%
! TGCTGGGAGTATGAAATTGG+GGG + chr2.4:35629161-35629180 MS.gene64383:intron 45.0%
!! TCCTCTCTTTGGGATACACT+TGG - chr2.4:35630758-35630777 None:intergenic 45.0%
ACGTAGTCATGGATCGTAGG+TGG + chr2.4:35630507-35630526 MS.gene64383:CDS 50.0%
AGCGTGAAATTGGACGGTGT+TGG + chr2.4:35629627-35629646 MS.gene64383:intron 50.0%
CGGGAAAATTTCGTGCATCC+TGG - chr2.4:35630899-35630918 None:intergenic 50.0%
CGTCCAATGGGTAGACAAGA+TGG + chr2.4:35631109-35631128 MS.gene64383:intron 50.0%
CTGGGACCCAAAACTTAGCA+AGG + chr2.4:35630652-35630671 MS.gene64383:CDS 50.0%
GCCAAGTGTATCCCAAAGAG+AGG + chr2.4:35630754-35630773 MS.gene64383:CDS 50.0%
GCGTGAAATTGGACGGTGTT+GGG + chr2.4:35629628-35629647 MS.gene64383:intron 50.0%
GGTCCTGGATCTTCTAATGC+AGG + chr2.4:35629700-35629719 MS.gene64383:intron 50.0%
TAATTCGGAGTTCGACCGTG+AGG - chr2.4:35629819-35629838 None:intergenic 50.0%
TCGACTTCCACGATTTCCTC+CGG - chr2.4:35629565-35629584 None:intergenic 50.0%
TGCCAGTGTCCATGATTCGT+AGG + chr2.4:35629063-35629082 MS.gene64383:CDS 50.0%
TTCGTGCATCCTGGGTACAA+AGG - chr2.4:35630890-35630909 None:intergenic 50.0%
! CTGGGAGTATGAAATTGGGG+GGG + chr2.4:35629163-35629182 MS.gene64383:intron 50.0%
! GCTGGGAGTATGAAATTGGG+GGG + chr2.4:35629162-35629181 MS.gene64383:intron 50.0%
! GCTTGTTGGGAGCGTGAAAT+TGG + chr2.4:35629617-35629636 MS.gene64383:intron 50.0%
! GTTGGGAGCGTGAAATTGGA+CGG + chr2.4:35629621-35629640 MS.gene64383:intron 50.0%
! TGGGAGTATGAAATTGGGGG+GGG + chr2.4:35629164-35629183 MS.gene64383:intron 50.0%
!! AGGGGCCTTCTAGTTTCGAA+AGG + chr2.4:35630683-35630702 MS.gene64383:CDS 50.0%
!! GGAAAAACTCCCTCGGTGTT+AGG - chr2.4:35630608-35630627 None:intergenic 50.0%
!! GGGGCCTTCTAGTTTCGAAA+GGG + chr2.4:35630684-35630703 MS.gene64383:CDS 50.0%
AGTATGAAATTGGGGGGGGG+GGG + chr2.4:35629168-35629187 MS.gene64383:intron 55.0%
GAGTATGAAATTGGGGGGGG+GGG + chr2.4:35629167-35629186 MS.gene64383:intron 55.0%
GGAGTATGAAATTGGGGGGG+GGG + chr2.4:35629166-35629185 MS.gene64383:intron 55.0%
! GGGAGTATGAAATTGGGGGG+GGG + chr2.4:35629165-35629184 MS.gene64383:intron 55.0%
AGTTGGGCCGGAGGAAATCG+TGG + chr2.4:35629555-35629574 MS.gene64383:intron 60.0%
GTATGAAATTGGGGGGGGGG+GGG + chr2.4:35629169-35629188 MS.gene64383:intron 60.0%
TATGAAATTGGGGGGGGGGG+GGG + chr2.4:35629170-35629189 MS.gene64383:intron 60.0%
TCCTCCGGCCCAACTCACAA+GGG - chr2.4:35629550-35629569 None:intergenic 60.0%
TTCCTCCGGCCCAACTCACA+AGG - chr2.4:35629551-35629570 None:intergenic 60.0%
! TAGGCCCTTGTGAGTTGGGC+CGG + chr2.4:35629543-35629562 MS.gene64383:intron 60.0%
ATGAAATTGGGGGGGGGGGG+GGG + chr2.4:35629171-35629190 MS.gene64383:intron 65.0%
TGAAATTGGGGGGGGGGGGG+GGG + chr2.4:35629172-35629191 MS.gene64383:intron 70.0%
! GCCCTTGTGAGTTGGGCCGG+AGG + chr2.4:35629546-35629565 MS.gene64383:intron 70.0%
AAATTGGGGGGGGGGGGGGG+GGG + chr2.4:35629174-35629193 MS.gene64383:intron 75.0%
GAAATTGGGGGGGGGGGGGG+GGG + chr2.4:35629173-35629192 MS.gene64383:intron 75.0%
! AATTGGGGGGGGGGGGGGGG+GGG + chr2.4:35629175-35629194 MS.gene64383:intron 80.0%
! ATTGGGGGGGGGGGGGGGGG+GGG + chr2.4:35629176-35629195 MS.gene64383:intron 85.0%
! GGGGGGGGGGGGGGGGGGTT+GGG + chr2.4:35629186-35629205 MS.gene64383:intron 90.0%
! GGGGGGGGGGGGGGGGGTTG+GGG + chr2.4:35629187-35629206 MS.gene64383:intron 90.0%
! TTGGGGGGGGGGGGGGGGGG+GGG + chr2.4:35629177-35629196 MS.gene64383:intron 90.0%
! GGGGGGGGGGGGGGGGGGGT+TGG + chr2.4:35629185-35629204 MS.gene64383:intron 95.0%
! TGGGGGGGGGGGGGGGGGGG+GGG + chr2.4:35629178-35629197 MS.gene64383:intron 95.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 35629026 35631451 35629026 ID=MS.gene64383
chr2.4 mRNA 35629026 35631451 35629026 ID=MS.gene64383.t1;Parent=MS.gene64383
chr2.4 exon 35629026 35629084 35629026 ID=MS.gene64383.t1.exon1;Parent=MS.gene64383.t1
chr2.4 CDS 35629026 35629084 35629026 ID=cds.MS.gene64383.t1;Parent=MS.gene64383.t1
chr2.4 exon 35630065 35630146 35630065 ID=MS.gene64383.t1.exon2;Parent=MS.gene64383.t1
chr2.4 CDS 35630065 35630146 35630065 ID=cds.MS.gene64383.t1;Parent=MS.gene64383.t1
chr2.4 exon 35630506 35631060 35630506 ID=MS.gene64383.t1.exon3;Parent=MS.gene64383.t1
chr2.4 CDS 35630506 35631060 35630506 ID=cds.MS.gene64383.t1;Parent=MS.gene64383.t1
chr2.4 exon 35631197 35631292 35631197 ID=MS.gene64383.t1.exon4;Parent=MS.gene64383.t1
chr2.4 CDS 35631197 35631292 35631197 ID=cds.MS.gene64383.t1;Parent=MS.gene64383.t1
chr2.4 exon 35631392 35631451 35631392 ID=MS.gene64383.t1.exon5;Parent=MS.gene64383.t1
chr2.4 CDS 35631392 35631451 35631392 ID=cds.MS.gene64383.t1;Parent=MS.gene64383.t1
Gene Sequence

>MS.gene64383

ATGAAGTCATTGTCTCAAAATTTCATCGGTGTTGACGTGCCAGTGTCCATGATTCGTAGGTATAGGAATGGTGGTAGGGTCACAATTTGGGCTTGTAGAAAAGTTATGTAGTCTTTTTATTTTATGTTGTTGCTTTGCTGGGAGTATGAAATTGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGGTGTTGTGACACGAAATCATTCTCTAAATTAAAATTTATTAAAACTTGTAACATCCTTACACATTCCTTACATTTTATGGACCACTCTTTTTAATCTGATTATGTGTTTTGGTTAAATGATGATTAAATGAGGTGGGTAGTCTTTTTATTTTATTTTATTTTAGACTATAGACCCATCTTAGTCCTTTACAATTTCTTTGTGGCTCAATTCAGTCTCCTACAAAAATAATACTAAATTAGTCCCTAATATTTTCAAATAGGGGACAAATTAGTCACTTGTTACAATGATTCAGAGACTAAACTGGATCCACTATGAACATGTTGGAGACTAATTTAGGCCCTTGTGAGTTGGGCCGGAGGAAATCGTGGAAGTCGAAATTGGGTCTTTATTCTTTATTATATGTTGTCGCTTGTTGGGAGCGTGAAATTGGACGGTGTTGGGACTTGAAATTGGTTTTATTTTCTGAAATATATCACAATTTAAATTTGAGGGTCCTGGATCTTCTAATGCAGGACTCACAGCAGCTCTCTATATCTCTTTAAATTTTTCTTATTCTCTTTCATATCTTTCCATTTTATTTCAATATTCTTGGGTAGGCTATCTTTACCTCACGGTCGAACTCCGAATTATCATTGTCCTTCTTGGATTGGTGGTGTAGTGTTGTCCGTAATTGGGTCGCCTTGTATTGCTTATAGGAAAAATATGGAATAAATATTTTTATATCTCTTCAATGTTGATTTGATGTTTTTTCTTTAAATACATCTTTGTGTTGTACTAATATCTGAATTCTCAATTGTTTTTTGAACAAACTGAACTCTCAATCTAAACTTTGGAAATGTTTTGATTTGTTAACAGAGCTCACAAGCACATCTTTTCCATTGCTGATTGGGTAGATAGCAAAAAGGTAGCTCTAGAAGCTGATCTCAAAAAAATTGAGGTAATTACTAACCTGATTCTCAACAAGATAACATTATATATCTTGTCTAACTAGTAGAACAAGTAGTTTGATGTTGAGATATTTGATGATCAGTTCATTTTGTCCTTTATGTGCATCATGTTGTTTTTGTCATATACTTATCTGGAGTGGTTTGAAGCAATTGTTATATATTATTGTATTATTTAGCTTTTTGAACTAGAATTGAAACAATACTACTATAGCAAACCAAACACAACAACAGAGAACCTATGGACCGTGCATGATGAAAACAAAGAATGCAGATTTTTTTTAACTACTTCTGTAGAATGAAAGCTTAGTAGGCATCCCAATTTTTTAATTTAGTATGTGTTAATTTTCAGAACGTAGTCATGGATCGTAGGTGGATGAAAGCTTATCGATTAAGTCGTGAGTATGATAATGGAGTGACTGAATTTCTACAATTTGCTGAAAAGAATCTTCCTAACACCGAGGGAGTTTTTCCATGTCCTTGTATTTCTTGTCGGAACTGGGACCCAAAACTTAGCAAGGAAGAAATTAGGGGCCTTCTAGTTTCGAAAGGGATTTGTCAAAATTATACCCAATGGATATGGCATGGTGAAACTATGATGCCAAGTGTATCCCAAAGAGAGGAAGGCAATGTAGATATGGATGATAGACTTGAAGGCATGATACGTGATATTGGAGAAGAATCATTCAAGAAAGCACATGTGTATGATAATTTATGTAAAGATAAGGAACAACCTTTGTACCCAGGATGCACGAAATTTTCCCGGCTGTCAGATGTGTTAAGATTGTTTAATTTGAAGGCAAAAAATGGATGGAGTGATAAAAGTTTCACTGAATTGCTTGGATTGTTGACAGAAATGCTTCCAGGAGGTAACACAATTCCAAATCGTCATTACGATGCCAAGAAAGTATTGTGTCCAATGGGTATGGAGTATGAAAAAATACATGCTAGACATCGTCCAATGGGTAGACAAGATGGTTACATTCGTAGTGATTTTTTTTATATATATCTATTCACTATATAAAAATTAAATTCATTTGTAGGCTAGGATGCAACCTAACACAACTGATTGTGGATATTATGTGATGAAGAACATGCTAGACATCGTCTCTGCTAATATTACTACCTCTTGGATGAAGGTATACCATATGTTTGCACCATAAATATTTCCAAATTAAGATAGCTAGACATATCCTTATTGTTATTTTACTTATTTTAATCTATTGTACAAACAGTAGGTATTCAATGATACAACAGCATTAACAGAAGATGAGTTGTATGCTTTGCGAAACGATTAG
Protein sequence

>MS.gene64383.t1

MKSLSQNFIGVDVPVSMIRRAHKHIFSIADWVDSKKVALEADLKKIENVVMDRRWMKAYRLSREYDNGVTEFLQFAEKNLPNTEGVFPCPCISCRNWDPKLSKEEIRGLLVSKGICQNYTQWIWHGETMMPSVSQREEGNVDMDDRLEGMIRDIGEESFKKAHVYDNLCKDKEQPLYPGCTKFSRLSDVLRLFNLKAKNGWSDKSFTELLGLLTEMLPGGNTIPNRHYDAKKARMQPNTTDCGYYVMKNMLDIVSANITTSWMKVFNDTTALTEDELYALRND