Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058657.t1 | XP_003624056.1 | 99.5 | 426 | 2 | 0 | 1 | 426 | 1 | 426 | 4.80E-242 | 846.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058657.t1 | Q8RX88 | 83.8 | 421 | 68 | 0 | 1 | 421 | 1 | 421 | 2.9e-203 | 709.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058657.t1 | G7L6L7 | 99.5 | 426 | 2 | 0 | 1 | 426 | 1 | 426 | 3.5e-242 | 846.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050076 | MS.gene058657 | -0.829209 | 5.85E-55 | -1.69E-46 |
| MS.gene058657 | MS.gene44005 | -0.800829 | 1.20E-48 | -1.69E-46 |
| MS.gene058657 | MS.gene85444 | 0.868346 | 6.99E-66 | -1.69E-46 |
| MS.gene058657 | MS.gene85540 | 0.863099 | 3.17E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058657.t1 | MTR_7g078760 | 99.531 | 426 | 2 | 0 | 1 | 426 | 1 | 426 | 0.0 | 869 |
| MS.gene058657.t1 | MTR_8g028680 | 59.132 | 438 | 157 | 5 | 1 | 421 | 1 | 433 | 1.93e-167 | 477 |
| MS.gene058657.t1 | MTR_4g065940 | 63.158 | 95 | 4 | 1 | 246 | 340 | 15 | 78 | 2.93e-28 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058657.t1 | AT4G01320 | 83.848 | 421 | 68 | 0 | 1 | 421 | 1 | 421 | 0.0 | 730 |
| MS.gene058657.t1 | AT4G01320 | 84.108 | 409 | 65 | 0 | 13 | 421 | 1 | 409 | 0.0 | 711 |
| MS.gene058657.t1 | AT4G01320 | 84.108 | 409 | 65 | 0 | 13 | 421 | 1 | 409 | 0.0 | 711 |
Find 89 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTGGACTCATCCTATTTC+AGG | 0.175438 | 7.4:+27181452 | MS.gene058657:CDS |
| GAGCAGATCATTTGAATTTC+AGG | 0.201726 | 7.4:+27182821 | MS.gene058657:CDS |
| ACTGATCTGTTCCAAAGTTT+TGG | 0.223216 | 7.4:+27181408 | MS.gene058657:CDS |
| AATTGGAGGACCAATTATTA+TGG | 0.255741 | 7.4:-27177929 | None:intergenic |
| ATTCTTACACTCTTGCAATT+TGG | 0.261444 | 7.4:+27181360 | MS.gene058657:CDS |
| CATACCTGGCGATCTATCTT+TGG | 0.277448 | 7.4:+27178184 | MS.gene058657:CDS |
| TCAAAGTATTGCCCTGGTTT+TGG | 0.280912 | 7.4:+27177199 | MS.gene058657:CDS |
| TTCTTCAGGGACATGATTAA+AGG | 0.282352 | 7.4:+27177895 | MS.gene058657:CDS |
| TATCTTTGGGCATTTATGTT+TGG | 0.289698 | 7.4:+27178198 | MS.gene058657:CDS |
| GTCCCGTCATGGCTTTAATA+AGG | 0.295172 | 7.4:+27177708 | MS.gene058657:CDS |
| AAGAAGTTGTTTGTTGTTGA+TGG | 0.299518 | 7.4:+27179494 | MS.gene058657:CDS |
| AAAGTTGAGTAAAGAGAAAA+AGG | 0.329340 | 7.4:-27177676 | None:intergenic |
| TATTCAAAAGCACCTGAAAT+AGG | 0.335673 | 7.4:-27181464 | None:intergenic |
| CTCCAGCAACTAGTCAGTTT+TGG | 0.346308 | 7.4:+27182786 | MS.gene058657:CDS |
| TCCTCCCCTTGTTGAAAGAT+TGG | 0.352311 | 7.4:+27183247 | MS.gene058657:CDS |
| TCACCAACGCATGAACAAAA+TGG | 0.356203 | 7.4:-27177146 | None:intergenic |
| AAGAGTGGAGCTATTAGAAT+TGG | 0.357379 | 7.4:-27178249 | None:intergenic |
| TCTGACTTCTCTGACTTATC+TGG | 0.360626 | 7.4:-27183284 | None:intergenic |
| ACTCTTCCAAAGTCTTTAGA+AGG | 0.369812 | 7.4:+27176902 | MS.gene058657:CDS |
| TAGATTTCTCAAATTTCTCT+TGG | 0.371414 | 7.4:-27176934 | None:intergenic |
| AGCTGGTCTTGTGAAATTAC+AGG | 0.384249 | 7.4:+27183077 | MS.gene058657:CDS |
| TTCTCTCTGAGTGGACCATC+TGG | 0.385423 | 7.4:-27179434 | None:intergenic |
| AAACACCATGGTTATTCTTC+AGG | 0.395992 | 7.4:+27177881 | MS.gene058657:CDS |
| GTCTATTGATTGCAGAAATC+AGG | 0.407821 | 7.4:+27177293 | MS.gene058657:intron |
| ATTACTCCTTCTAAAGACTT+TGG | 0.412273 | 7.4:-27176908 | None:intergenic |
| ATACCTGGCGATCTATCTTT+GGG | 0.416163 | 7.4:+27178185 | MS.gene058657:CDS |
| GACCAAAACTGACTAGTTGC+TGG | 0.416907 | 7.4:-27182788 | None:intergenic |
| TTATGACACATTAGTTCAAC+AGG | 0.417625 | 7.4:+27179741 | MS.gene058657:CDS |
| CAACCTCTTATCAAGACTAT+AGG | 0.418001 | 7.4:-27176960 | None:intergenic |
| CAATGAAGGTGTACACAGTA+TGG | 0.427524 | 7.4:-27180899 | None:intergenic |
| AATGAAATATTTCATACCCT+TGG | 0.427931 | 7.4:+27177350 | MS.gene058657:CDS |
| ACAATGATTGCAGCCACAAT+TGG | 0.433177 | 7.4:-27177946 | None:intergenic |
| ACAACAAACAACTTCTTCAA+TGG | 0.442140 | 7.4:-27179488 | None:intergenic |
| CCCTACATGGAAGCTGTTGT+CGG | 0.445864 | 7.4:+27175713 | MS.gene058657:CDS |
| GTACACCTTCATTGCTATGC+AGG | 0.450398 | 7.4:+27180908 | MS.gene058657:CDS |
| CTTGAAGAATCCATACATAT+AGG | 0.451579 | 7.4:-27179701 | None:intergenic |
| AACCATGGCGTTTCCCTACA+TGG | 0.451982 | 7.4:+27175700 | None:intergenic |
| ACTTCGCAGGCCTATATGTA+TGG | 0.453143 | 7.4:+27179691 | MS.gene058657:intron |
| ATGCCCAAAGATAGATCGCC+AGG | 0.460835 | 7.4:-27178188 | None:intergenic |
| GATACACAGCCAGTCCTCAT+TGG | 0.461646 | 7.4:+27181435 | MS.gene058657:CDS |
| GCTGTCATTGCTCATGAGCT+TGG | 0.464174 | 7.4:+27180861 | MS.gene058657:CDS |
| CTTACACTCTTGCAATTTGG+AGG | 0.464916 | 7.4:+27181363 | MS.gene058657:CDS |
| CTTCTTCAATGGAAAATTGA+GGG | 0.466372 | 7.4:-27179477 | None:intergenic |
| CAAAGATAGATCGCCAGGTA+TGG | 0.469501 | 7.4:-27178183 | None:intergenic |
| GATGGCTTTGCTAAGAAGCT+TGG | 0.475579 | 7.4:+27183036 | MS.gene058657:CDS |
| AACACCATGGTTATTCTTCA+GGG | 0.485333 | 7.4:+27177882 | MS.gene058657:CDS |
| GTGTATCAAACCCAAAACTT+TGG | 0.493484 | 7.4:-27181419 | None:intergenic |
| TGCAATCATTGTAATAGTAC+AGG | 0.497010 | 7.4:+27177957 | MS.gene058657:CDS |
| ACTTCTTCAATGGAAAATTG+AGG | 0.499325 | 7.4:-27179478 | None:intergenic |
| TTGCTCATGAGCTTGGACAC+TGG | 0.501838 | 7.4:+27180868 | MS.gene058657:CDS |
| CTTGGATTTGCGACAACACA+AGG | 0.503174 | 7.4:+27176865 | MS.gene058657:CDS |
| GAGCCTATAGTCTTGATAAG+AGG | 0.511670 | 7.4:+27176957 | MS.gene058657:CDS |
| ACAAACCTGCATAGCAATGA+AGG | 0.515003 | 7.4:-27180913 | None:intergenic |
| TCTAAAGACTTTGGAAGAGT+AGG | 0.516883 | 7.4:-27176899 | None:intergenic |
| ATTGCTGTGACTTGATCTTG+TGG | 0.518498 | 7.4:-27179519 | None:intergenic |
| CCGACAACAGCTTCCATGTA+GGG | 0.525236 | 7.4:-27175713 | None:intergenic |
| TATCTTCCTACGTAGGCTGA+TGG | 0.540706 | 7.4:+27183018 | MS.gene058657:intron |
| TGTACTTCAAAGTATTGCCC+TGG | 0.546955 | 7.4:+27177193 | MS.gene058657:CDS |
| AATTCTCACCTTCCAAAACC+AGG | 0.548885 | 7.4:-27177211 | None:intergenic |
| ATGATTGCAGCCACAATTGG+AGG | 0.553279 | 7.4:-27177943 | None:intergenic |
| TATTCATCTTCGCTACGAGC+TGG | 0.555436 | 7.4:+27183060 | MS.gene058657:CDS |
| ATTCAAATGATCTGCTCACA+AGG | 0.557840 | 7.4:-27182815 | None:intergenic |
| AATAATTGGTCCTCCAATTG+TGG | 0.559277 | 7.4:+27177933 | MS.gene058657:CDS |
| TGGTTTGATGATCTGGTCAC+AGG | 0.559653 | 7.4:+27177382 | MS.gene058657:CDS |
| TTTGAATATTTGCAGAAAGG+AGG | 0.560706 | 7.4:+27178159 | MS.gene058657:intron |
| AACCTTATTAAAGCCATGAC+GGG | 0.563471 | 7.4:-27177710 | None:intergenic |
| AACTGACTAGTTGCTGGAGT+GGG | 0.569989 | 7.4:-27182782 | None:intergenic |
| GCAGAAAGGAGGTCCATACC+TGG | 0.570962 | 7.4:+27178170 | MS.gene058657:intron |
| TTTGTGATTGAGTCCCGTCA+TGG | 0.571395 | 7.4:+27177697 | MS.gene058657:CDS |
| ACGCATGAACAAAATGGAAG+TGG | 0.573573 | 7.4:-27177140 | None:intergenic |
| AGGATGAGTCCAATGAGGAC+TGG | 0.592039 | 7.4:-27181444 | None:intergenic |
| GTGCAGCCAATCTTTCAACA+AGG | 0.601766 | 7.4:-27183253 | None:intergenic |
| ATGGATTCTTCAAGAACAAG+AGG | 0.602414 | 7.4:+27179710 | MS.gene058657:CDS |
| GCCAATCTTTCAACAAGGGG+AGG | 0.604396 | 7.4:-27183248 | None:intergenic |
| TCTGGTAACCAGCTTCCAGA+TGG | 0.616397 | 7.4:+27179419 | MS.gene058657:intron |
| TGCAGCCAATCTTTCAACAA+GGG | 0.616439 | 7.4:-27183252 | None:intergenic |
| CAGCTATGAATACAGATCCA+TGG | 0.622966 | 7.4:+27183201 | MS.gene058657:CDS |
| ACCGACAACAGCTTCCATGT+AGG | 0.631757 | 7.4:-27175714 | None:intergenic |
| GGAGTGAACTTATTGAAGAG+TGG | 0.635001 | 7.4:-27178264 | None:intergenic |
| GCAGCCAATCTTTCAACAAG+GGG | 0.637075 | 7.4:-27183251 | None:intergenic |
| AAACTGACTAGTTGCTGGAG+TGG | 0.637131 | 7.4:-27182783 | None:intergenic |
| GAACCTTATTAAAGCCATGA+CGG | 0.641005 | 7.4:-27177711 | None:intergenic |
| CTTCAATGGAAAATTGAGGG+AGG | 0.644359 | 7.4:-27179474 | None:intergenic |
| ATTCTCACCTTCCAAAACCA+GGG | 0.646241 | 7.4:-27177210 | None:intergenic |
| TAGTGATAAGCAGAGTACCA+TGG | 0.657764 | 7.4:-27183218 | None:intergenic |
| TTCCATGTAGGGAAACGCCA+TGG | 0.667003 | 7.4:-27175702 | None:intergenic |
| ATGTCCCTGAAGAATAACCA+TGG | 0.683859 | 7.4:-27177886 | None:intergenic |
| GAAATAGGATGAGTCCAATG+AGG | 0.707590 | 7.4:-27181449 | None:intergenic |
| CTATCCTACAGCAAACACCA+TGG | 0.720457 | 7.4:+27177869 | MS.gene058657:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 27175704 | 27183310 | 27175704 | ID=MS.gene058657 |
| chr7.4 | mRNA | 27175704 | 27183310 | 27175704 | ID=MS.gene058657.t1;Parent=MS.gene058657 |
| chr7.4 | exon | 27175704 | 27175734 | 27175704 | ID=MS.gene058657.t1.exon1;Parent=MS.gene058657.t1 |
| chr7.4 | CDS | 27175704 | 27175734 | 27175704 | ID=cds.MS.gene058657.t1;Parent=MS.gene058657.t1 |
| chr7.4 | exon | 27176831 | 27176978 | 27176831 | ID=MS.gene058657.t1.exon2;Parent=MS.gene058657.t1 |
| chr7.4 | CDS | 27176831 | 27176978 | 27176831 | ID=cds.MS.gene058657.t1;Parent=MS.gene058657.t1 |
| chr7.4 | exon | 27177140 | 27177224 | 27177140 | ID=MS.gene058657.t1.exon3;Parent=MS.gene058657.t1 |
| chr7.4 | CDS | 27177140 | 27177224 | 27177140 | ID=cds.MS.gene058657.t1;Parent=MS.gene058657.t1 |
| chr7.4 | exon | 27177308 | 27177403 | 27177308 | ID=MS.gene058657.t1.exon4;Parent=MS.gene058657.t1 |
| chr7.4 | CDS | 27177308 | 27177403 | 27177308 | ID=cds.MS.gene058657.t1;Parent=MS.gene058657.t1 |
| chr7.4 | exon | 27177664 | 27177729 | 27177664 | ID=MS.gene058657.t1.exon5;Parent=MS.gene058657.t1 |
| chr7.4 | CDS | 27177664 | 27177729 | 27177664 | ID=cds.MS.gene058657.t1;Parent=MS.gene058657.t1 |
| chr7.4 | exon | 27177880 | 27177978 | 27177880 | ID=MS.gene058657.t1.exon6;Parent=MS.gene058657.t1 |
| chr7.4 | CDS | 27177880 | 27177978 | 27177880 | ID=cds.MS.gene058657.t1;Parent=MS.gene058657.t1 |
| chr7.4 | exon | 27178174 | 27178287 | 27178174 | ID=MS.gene058657.t1.exon7;Parent=MS.gene058657.t1 |
| chr7.4 | CDS | 27178174 | 27178287 | 27178174 | ID=cds.MS.gene058657.t1;Parent=MS.gene058657.t1 |
| chr7.4 | exon | 27179431 | 27179541 | 27179431 | ID=MS.gene058657.t1.exon8;Parent=MS.gene058657.t1 |
| chr7.4 | CDS | 27179431 | 27179541 | 27179431 | ID=cds.MS.gene058657.t1;Parent=MS.gene058657.t1 |
| chr7.4 | exon | 27179700 | 27179762 | 27179700 | ID=MS.gene058657.t1.exon9;Parent=MS.gene058657.t1 |
| chr7.4 | CDS | 27179700 | 27179762 | 27179700 | ID=cds.MS.gene058657.t1;Parent=MS.gene058657.t1 |
| chr7.4 | exon | 27180837 | 27180929 | 27180837 | ID=MS.gene058657.t1.exon10;Parent=MS.gene058657.t1 |
| chr7.4 | CDS | 27180837 | 27180929 | 27180837 | ID=cds.MS.gene058657.t1;Parent=MS.gene058657.t1 |
| chr7.4 | exon | 27181360 | 27181473 | 27181360 | ID=MS.gene058657.t1.exon11;Parent=MS.gene058657.t1 |
| chr7.4 | CDS | 27181360 | 27181473 | 27181360 | ID=cds.MS.gene058657.t1;Parent=MS.gene058657.t1 |
| chr7.4 | exon | 27182771 | 27182842 | 27182771 | ID=MS.gene058657.t1.exon12;Parent=MS.gene058657.t1 |
| chr7.4 | CDS | 27182771 | 27182842 | 27182771 | ID=cds.MS.gene058657.t1;Parent=MS.gene058657.t1 |
| chr7.4 | exon | 27183033 | 27183098 | 27183033 | ID=MS.gene058657.t1.exon13;Parent=MS.gene058657.t1 |
| chr7.4 | CDS | 27183033 | 27183098 | 27183033 | ID=cds.MS.gene058657.t1;Parent=MS.gene058657.t1 |
| chr7.4 | exon | 27183188 | 27183310 | 27183188 | ID=MS.gene058657.t1.exon14;Parent=MS.gene058657.t1 |
| chr7.4 | CDS | 27183188 | 27183310 | 27183188 | ID=cds.MS.gene058657.t1;Parent=MS.gene058657.t1 |
| Gene Sequence |
| Protein sequence |
