AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44005


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44005.t1 MTR_1g080910 90.210 143 7 2 1 140 1 139 8.74e-73 214
MS.gene44005.t1 MTR_1g080910 88.462 104 5 2 1 101 1 100 6.52e-44 140
MS.gene44005.t1 MTR_7g115180 70.707 99 24 2 47 140 46 144 7.08e-41 133
MS.gene44005.t1 MTR_7g115180 68.919 74 21 1 47 118 46 119 5.03e-27 97.8
MS.gene44005.t1 MTR_1g100220 40.260 77 45 1 49 125 75 150 9.29e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44005.t1 AT5G28750 55.652 115 43 3 27 136 31 142 2.95e-36 122
MS.gene44005.t1 AT5G52440 52.083 48 23 0 60 107 85 132 3.48e-11 59.3

Find 31 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGCTTTGTGCTTCTGTTTC+TGG 0.042249 1.3:+57331242 None:intergenic
CCAACTTCGGGCAATTTCTT+AGG 0.195754 1.3:+57331141 None:intergenic
TCTGGTTCTGGTTCTGGTTC+TGG 0.213224 1.3:+57331260 None:intergenic
TCTGTTTCTGGTTCTGGTTC+TGG 0.213548 1.3:+57331254 None:intergenic
TGTGCTTCTGTTTCTGGTTC+TGG 0.214615 1.3:+57331248 None:intergenic
CATTATTGTCTTGATCTTCT+TGG 0.219177 1.3:+57330702 None:intergenic
GAAATTTCTTCAGTAGAATT+AGG 0.222712 1.3:+57330737 None:intergenic
CCCGAGCTTGTTGTTATTGC+AGG 0.253511 1.3:-57331186 MS.gene44005:CDS
GCCGATGCTGCGACCAACTT+CGG 0.342708 1.3:+57331128 None:intergenic
TTTACTTGCAACGCCTTCTT+CGG 0.349544 1.3:-57331219 MS.gene44005:CDS
AAGTGATGTATTGTTAGAAT+TGG 0.354759 1.3:+57331296 None:intergenic
CCGATGCTGCGACCAACTTC+GGG 0.355688 1.3:+57331129 None:intergenic
TAAGAAGGATAAGGTGGAAT+AGG 0.383721 1.3:+57331323 None:intergenic
GTTAGAATTGGAAGCTAAGA+AGG 0.407354 1.3:+57331308 None:intergenic
GTGGAATAGGATGAAGAGAT+TGG 0.421445 1.3:+57331336 None:intergenic
GCGAATTAAAGAAGAAGAAA+GGG 0.424605 1.3:+57331368 None:intergenic
CTTCTTCTTTAATTCGCCCT+AGG 0.426973 1.3:-57331362 MS.gene44005:CDS
GGCGAATTAAAGAAGAAGAA+AGG 0.452383 1.3:+57331367 None:intergenic
AACTGTCAAAAGCTTCCAAC+AGG 0.455252 1.3:-57331103 MS.gene44005:intron
CCCGAAGTTGGTCGCAGCAT+CGG 0.463383 1.3:-57331129 MS.gene44005:CDS
CCTAAGAAATTGCCCGAAGT+TGG 0.474122 1.3:-57331141 MS.gene44005:CDS
ATTGGAAGCTAAGAAGGATA+AGG 0.493246 1.3:+57331314 None:intergenic
TGAAACCTTGTAGGCAGCAA+AGG 0.513076 1.3:-57330786 MS.gene44005:intron
TGCAACGCCTTCTTCGGATT+AGG 0.521226 1.3:-57331213 MS.gene44005:CDS
TCCTGCAATAACAACAAGCT+CGG 0.534091 1.3:+57331185 None:intergenic
CAATAATGTTTCTAGTACAA+AGG 0.541741 1.3:-57330687 MS.gene44005:CDS
ATGAAGAGATTGGAAGCCTA+GGG 0.550257 1.3:+57331346 None:intergenic
GATGAAGAGATTGGAAGCCT+AGG 0.550349 1.3:+57331345 None:intergenic
CCTGCAATAACAACAAGCTC+GGG 0.555493 1.3:+57331186 None:intergenic
GGAAGCTAAGAAGGATAAGG+TGG 0.578078 1.3:+57331317 None:intergenic
GGGCACGCCTAATCCGAAGA+AGG 0.614059 1.3:+57331206 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTCTTATTATCTAATTAA+CGG - chr1.3:57331057-57331076 MS.gene44005:intron 10.0%
!!! TAAAATTGAAATTGTTTTAT+AGG - chr1.3:57331146-57331165 MS.gene44005:CDS 10.0%
!!! CAAATTTTTATTGTTTGTTT+GGG - chr1.3:57331236-57331255 MS.gene44005:CDS 15.0%
!!! GCAAATTTTTATTGTTTGTT+TGG - chr1.3:57331235-57331254 MS.gene44005:CDS 20.0%
! AAGTGATGTATTGTTAGAAT+TGG + chr1.3:57330767-57330786 None:intergenic 25.0%
! GAAATTTCTTCAGTAGAATT+AGG + chr1.3:57331326-57331345 None:intergenic 25.0%
! TTATCCTAAGAATGAGAAAA+TGG + chr1.3:57330985-57331004 None:intergenic 25.0%
!!! ATTAGATTTTGAAACCTTGT+AGG - chr1.3:57331265-57331284 MS.gene44005:CDS 25.0%
!!! ATTAGGTTCCTTTTTTAACT+CGG + chr1.3:57331309-57331328 None:intergenic 25.0%
!!! CAATAATGTTTCTAGTACAA+AGG - chr1.3:57331373-57331392 MS.gene44005:CDS 25.0%
ATGTAGAACTCCTATATGTT+AGG + chr1.3:57331032-57331051 None:intergenic 30.0%
CATTATTGTCTTGATCTTCT+TGG + chr1.3:57331361-57331380 None:intergenic 30.0%
GCGAATTAAAGAAGAAGAAA+GGG + chr1.3:57330695-57330714 None:intergenic 30.0%
TCATTCGTTGTATTTGAATG+AGG - chr1.3:57331190-57331209 MS.gene44005:CDS 30.0%
!! ATTTGTTGTGCCTAACATAT+AGG - chr1.3:57331019-57331038 MS.gene44005:intron 30.0%
AATGAGAAAATGGAACCTGT+TGG + chr1.3:57330975-57330994 None:intergenic 35.0%
ATTGGAAGCTAAGAAGGATA+AGG + chr1.3:57330749-57330768 None:intergenic 35.0%
GGCGAATTAAAGAAGAAGAA+AGG + chr1.3:57330696-57330715 None:intergenic 35.0%
GTTAGAATTGGAAGCTAAGA+AGG + chr1.3:57330755-57330774 None:intergenic 35.0%
TAAGAAGGATAAGGTGGAAT+AGG + chr1.3:57330740-57330759 None:intergenic 35.0%
! GGTTCCATTTTCTCATTCTT+AGG - chr1.3:57330978-57330997 MS.gene44005:intron 35.0%
! GTTTGAGACCGAGTTAAAAA+AGG - chr1.3:57331298-57331317 MS.gene44005:CDS 35.0%
AACTGTCAAAAGCTTCCAAC+AGG - chr1.3:57330957-57330976 MS.gene44005:intron 40.0%
ATGAAGAGATTGGAAGCCTA+GGG + chr1.3:57330717-57330736 None:intergenic 40.0%
CTTCTTCTTTAATTCGCCCT+AGG - chr1.3:57330698-57330717 MS.gene44005:CDS 40.0%
GTGGAATAGGATGAAGAGAT+TGG + chr1.3:57330727-57330746 None:intergenic 40.0%
TCCTGCAATAACAACAAGCT+CGG + chr1.3:57330878-57330897 None:intergenic 40.0%
TTTACTTGCAACGCCTTCTT+CGG - chr1.3:57330841-57330860 MS.gene44005:intron 40.0%
AAACTCCTTTGCTGCCTACA+AGG + chr1.3:57331282-57331301 None:intergenic 45.0%
ACGCTTTGTGCTTCTGTTTC+TGG + chr1.3:57330821-57330840 None:intergenic 45.0%
CCAACTTCGGGCAATTTCTT+AGG + chr1.3:57330922-57330941 None:intergenic 45.0%
CCTAAGAAATTGCCCGAAGT+TGG - chr1.3:57330919-57330938 MS.gene44005:intron 45.0%
CCTGCAATAACAACAAGCTC+GGG + chr1.3:57330877-57330896 None:intergenic 45.0%
GATGAAGAGATTGGAAGCCT+AGG + chr1.3:57330718-57330737 None:intergenic 45.0%
GGAAGCTAAGAAGGATAAGG+TGG + chr1.3:57330746-57330765 None:intergenic 45.0%
TGAAACCTTGTAGGCAGCAA+AGG - chr1.3:57331274-57331293 MS.gene44005:CDS 45.0%
!! TCTGTTTCTGGTTCTGGTTC+TGG + chr1.3:57330809-57330828 None:intergenic 45.0%
!! TGTGCTTCTGTTTCTGGTTC+TGG + chr1.3:57330815-57330834 None:intergenic 45.0%
TGCAACGCCTTCTTCGGATT+AGG - chr1.3:57330847-57330866 MS.gene44005:intron 50.0%
!! CCCGAGCTTGTTGTTATTGC+AGG - chr1.3:57330874-57330893 MS.gene44005:intron 50.0%
!! TCTGGTTCTGGTTCTGGTTC+TGG + chr1.3:57330803-57330822 None:intergenic 50.0%
!!! GGAGTCGCTGCTCTTGTTTT+CGG - chr1.3:57330895-57330914 MS.gene44005:intron 50.0%
CCGATGCTGCGACCAACTTC+GGG + chr1.3:57330934-57330953 None:intergenic 60.0%
GCCGATGCTGCGACCAACTT+CGG + chr1.3:57330935-57330954 None:intergenic 60.0%
GGGCACGCCTAATCCGAAGA+AGG + chr1.3:57330857-57330876 None:intergenic 60.0%
! CCCGAAGTTGGTCGCAGCAT+CGG - chr1.3:57330931-57330950 MS.gene44005:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 57330676 57331406 57330676 ID=MS.gene44005
chr1.3 mRNA 57330676 57331406 57330676 ID=MS.gene44005.t1;Parent=MS.gene44005
chr1.3 exon 57331104 57331406 57331104 ID=MS.gene44005.t1.exon1;Parent=MS.gene44005.t1
chr1.3 CDS 57331104 57331406 57331104 ID=cds.MS.gene44005.t1;Parent=MS.gene44005.t1
chr1.3 exon 57330676 57330795 57330676 ID=MS.gene44005.t1.exon2;Parent=MS.gene44005.t1
chr1.3 CDS 57330676 57330795 57330676 ID=cds.MS.gene44005.t1;Parent=MS.gene44005.t1
Gene Sequence

>MS.gene44005

ATGGAGACAACACTTTCCCTTTCTTCTTCTTTAATTCGCCCTAGGCTTCCAATCTCTTCATCCTATTCCACCTTATCCTTCTTAGCTTCCAATTCTAACAATACATCACTTCTCTTGAAGAAAGCCAGAACCAGAACCAGAACCAGAAACAGAAGCACAAAGCGTTTTACTTGCAACGCCTTCTTCGGATTAGGCGTGCCCGAGCTTGTTGTTATTGCAGGAGTCGCTGCTCTTGTTTTCGGTCCTAAGAAATTGCCCGAAGTTGGTCGCAGCATCGGCAAAACTGTCAAAAGCTTCCAACAGGTTCCATTTTCTCATTCTTAGGATAAGTTGCATTTTTTTTATTTGTTGTGCCTAACATATAGGAGTTCTACATTTGCAATTTCTTATTATCTAATTAACGGATCATATTTTTTTTAAATAATCGCATAAAATTTTGTAATATGTATATCTATCTTAAATTAGTCGCGTAAAATTGAAATTGTTTTATAGGTTCATTTGTATTTGTACTTACTCATTCGTTGTATTTGAATGAGGAGTTTTTAATAATTCAGTTGAAGCAAATTTTTATTGTTTGTTTGGGTTACTTATTAGATTTTGAAACCTTGTAGGCAGCAAAGGAGTTTGAGACCGAGTTAAAAAAGGAACCTAATTCTACTGAAGAAATTTCTGTTGCGAATGACCAAGAAGATCAAGACAATAATGTTTCTAGTACAAAGGATAGTGTATGA

Protein sequence

>MS.gene44005.t1

METTLSLSSSLIRPRLPISSSYSTLSFLASNSNNTSLLLKKARTRTRTRNRSTKRFTCNAFFGLGVPELVVIAGVAALVFGPKKLPEVGRSIGKTVKSFQQAAKEFETELKKEPNSTEEISVANDQEDQDNNVSSTKDSV