Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059165.t1 | GAU22899.1 | 88.4 | 95 | 11 | 0 | 1 | 95 | 1 | 95 | 3.20E-37 | 164.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059165.t1 | A0A2Z6MVA7 | 88.4 | 95 | 11 | 0 | 1 | 95 | 1 | 95 | 2.3e-37 | 164.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059165 | MS.gene25077 | 0.800598 | 1.34E-48 | -1.69E-46 |
| MS.gene059165 | MS.gene43491 | 0.808839 | 2.54E-50 | -1.69E-46 |
| MS.gene059165 | MS.gene54553 | 0.993329 | 5.51E-199 | -1.69E-46 |
| MS.gene059165 | MS.gene67884 | 0.802922 | 4.46E-49 | -1.69E-46 |
| MS.gene059165 | MS.gene95039 | 0.809916 | 1.49E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059165.t1 | MTR_1g007870 | 90.526 | 95 | 7 | 1 | 1 | 95 | 1 | 93 | 6.64e-58 | 173 |
| MS.gene059165.t1 | MTR_3g091850 | 88.421 | 95 | 11 | 0 | 1 | 95 | 1 | 95 | 1.38e-57 | 172 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059165.t1 | AT5G53045 | 70.787 | 89 | 26 | 0 | 3 | 91 | 7 | 95 | 2.92e-42 | 133 |
Find 23 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCAAAACAATTGAAGAATT+TGG | 0.196365 | 1.2:+11907435 | MS.gene059165:CDS |
| ACCAAATTCTTCAATTGTTT+TGG | 0.227496 | 1.2:-11907436 | None:intergenic |
| AGTGACTTGGAAGTGGATTT+TGG | 0.302231 | 1.2:+11906590 | MS.gene059165:intron |
| CGACTCATGACGGTGTCTAA+TGG | 0.332701 | 1.2:+11906776 | MS.gene059165:CDS |
| ATGAGTCGTTTAACTTTGTC+AGG | 0.393714 | 1.2:-11906761 | None:intergenic |
| GAATGATGCTCGAAGAAATC+TGG | 0.394790 | 1.2:-11907382 | None:intergenic |
| CTTTATATTTAGGCACTTTG+AGG | 0.444146 | 1.2:+11907350 | MS.gene059165:intron |
| TGCTGCCTTAGCTGTGGATG+AGG | 0.502660 | 1.2:+11906640 | MS.gene059165:CDS |
| TGAAGAATTTGGTCAAGGAA+TGG | 0.505507 | 1.2:+11907446 | MS.gene059165:CDS |
| AAACCTGGTAAAATCCCATT+GGG | 0.510542 | 1.2:-11906232 | None:intergenic |
| TGGTAAAATCCCATTGGGAT+TGG | 0.519873 | 1.2:-11906227 | None:intergenic |
| ACCACACAACCAATCCCAAT+GGG | 0.522672 | 1.2:+11906218 | MS.gene059165:CDS |
| GAATGACAATTGCAGTGACT+TGG | 0.538867 | 1.2:+11906577 | MS.gene059165:intron |
| GAAACCTGGTAAAATCCCAT+TGG | 0.539649 | 1.2:-11906233 | None:intergenic |
| CAATTGCAGTGACTTGGAAG+TGG | 0.541986 | 1.2:+11906583 | MS.gene059165:intron |
| CACCTCCTCATCCACAGCTA+AGG | 0.544761 | 1.2:-11906645 | None:intergenic |
| TTGGGATTGGTTGTGTGGTG+TGG | 0.553170 | 1.2:-11906214 | None:intergenic |
| TCCCATTGGGATTGGTTGTG+TGG | 0.555381 | 1.2:-11906219 | None:intergenic |
| ACAATTGAAGAATTTGGTCA+AGG | 0.561595 | 1.2:+11907441 | MS.gene059165:CDS |
| CACCACACAACCAATCCCAA+TGG | 0.580515 | 1.2:+11906217 | MS.gene059165:CDS |
| TGTATATGCTGCCTTAGCTG+TGG | 0.587580 | 1.2:+11906634 | MS.gene059165:CDS |
| CAAAGTTAAACGACTCATGA+CGG | 0.619519 | 1.2:+11906766 | MS.gene059165:CDS |
| TGCCTTAGCTGTGGATGAGG+AGG | 0.626509 | 1.2:+11906643 | MS.gene059165:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGTTATTTTTAATAAAGA+TGG | + | chr1.2:11906555-11906574 | MS.gene059165:intron | 10.0% |
| !!! | TAATTATTTTAAAGAATTGT+GGG | + | chr1.2:11906488-11906507 | MS.gene059165:intron | 10.0% |
| !!! | TTAATTATTTTAAAGAATTG+TGG | + | chr1.2:11906487-11906506 | MS.gene059165:intron | 10.0% |
| !! | AGATTAAATTAATTAATCAC+TGG | - | chr1.2:11906328-11906347 | None:intergenic | 15.0% |
| !!! | ATTAGTTAATTTAGCTTTAA+TGG | + | chr1.2:11907266-11907285 | MS.gene059165:intron | 15.0% |
| !! | AGCTTATTTACATAATTTCT+AGG | - | chr1.2:11906390-11906409 | None:intergenic | 20.0% |
| !! | GCTTATTTACATAATTTCTA+GGG | - | chr1.2:11906389-11906408 | None:intergenic | 20.0% |
| ! | AAAAAGCAAGAAAACTTGAT+AGG | - | chr1.2:11906286-11906305 | None:intergenic | 25.0% |
| ! | ACCAAAACAATTGAAGAATT+TGG | + | chr1.2:11907435-11907454 | MS.gene059165:CDS | 25.0% |
| ! | CATTGGAATCCTTTATATTT+AGG | + | chr1.2:11907340-11907359 | MS.gene059165:intron | 25.0% |
| !! | ACCAAATTCTTCAATTGTTT+TGG | - | chr1.2:11907439-11907458 | None:intergenic | 25.0% |
| !! | GATTTCTTTTGTCTTATGAA+CGG | - | chr1.2:11906461-11906480 | None:intergenic | 25.0% |
| !!! | ACTGCAATGATTTTTTTTGT+TGG | + | chr1.2:11907193-11907212 | MS.gene059165:intron | 25.0% |
| AACCAATTATCAACACGAAA+AGG | - | chr1.2:11906823-11906842 | None:intergenic | 30.0% | |
| ACAATTGAAGAATTTGGTCA+AGG | + | chr1.2:11907441-11907460 | MS.gene059165:CDS | 30.0% | |
| CAGTTAGTGACAAATTGTTT+AGG | - | chr1.2:11907116-11907135 | None:intergenic | 30.0% | |
| CTTTATATTTAGGCACTTTG+AGG | + | chr1.2:11907350-11907369 | MS.gene059165:intron | 30.0% | |
| TTCCAATGAGATGAAAAACT+AGG | - | chr1.2:11907328-11907347 | None:intergenic | 30.0% | |
| ! | CTCAAAGTGCCTAAATATAA+AGG | - | chr1.2:11907352-11907371 | None:intergenic | 30.0% |
| !!! | GTTCAATGATTGTTTTAGTC+AGG | + | chr1.2:11907073-11907092 | MS.gene059165:intron | 30.0% |
| !!! | TTCCTTTTCGTGTTGATAAT+TGG | + | chr1.2:11906818-11906837 | MS.gene059165:intron | 30.0% |
| AAACCTGGTAAAATCCCATT+GGG | - | chr1.2:11906235-11906254 | None:intergenic | 35.0% | |
| CAAAGTTAAACGACTCATGA+CGG | + | chr1.2:11906766-11906785 | MS.gene059165:CDS | 35.0% | |
| TCATTGTACTGCATCATGTT+TGG | + | chr1.2:11907166-11907185 | MS.gene059165:intron | 35.0% | |
| TCTTTCTCACGAGAATAAGA+GGG | - | chr1.2:11907019-11907038 | None:intergenic | 35.0% | |
| TGAAGAATTTGGTCAAGGAA+TGG | + | chr1.2:11907446-11907465 | MS.gene059165:CDS | 35.0% | |
| TTCTTTCTCACGAGAATAAG+AGG | - | chr1.2:11907020-11907039 | None:intergenic | 35.0% | |
| TTTAGTCAGGATTTGAACTC+AGG | + | chr1.2:11907086-11907105 | MS.gene059165:intron | 35.0% | |
| ! | ATGAGTCGTTTAACTTTGTC+AGG | - | chr1.2:11906764-11906783 | None:intergenic | 35.0% |
| ! | TGTAATCAATTTTGTGTGCG+AGG | + | chr1.2:11906929-11906948 | MS.gene059165:intron | 35.0% |
| ! | TTTTGTAGATGTCCTTACTC+TGG | + | chr1.2:11907410-11907429 | MS.gene059165:CDS | 35.0% |
| !! | TGCCTAGTTTTTCATCTCAT+TGG | + | chr1.2:11907323-11907342 | MS.gene059165:intron | 35.0% |
| AGAAAGAAGCCCTAGTAATC+CGG | + | chr1.2:11907032-11907051 | MS.gene059165:intron | 40.0% | |
| AGAGTAAAGAGAGAGAAACC+TGG | - | chr1.2:11906250-11906269 | None:intergenic | 40.0% | |
| ATGAAGTGGTTGGACTCAAA+TGG | - | chr1.2:11907150-11907169 | None:intergenic | 40.0% | |
| ATGCAGTACAATGAAGTGGT+TGG | - | chr1.2:11907160-11907179 | None:intergenic | 40.0% | |
| CAGACTTTACTTAACACCAC+CGG | - | chr1.2:11907054-11907073 | None:intergenic | 40.0% | |
| CATGATGCAGTACAATGAAG+TGG | - | chr1.2:11907164-11907183 | None:intergenic | 40.0% | |
| GAAACCTGGTAAAATCCCAT+TGG | - | chr1.2:11906236-11906255 | None:intergenic | 40.0% | |
| GAATGACAATTGCAGTGACT+TGG | + | chr1.2:11906577-11906596 | MS.gene059165:intron | 40.0% | |
| GAATGATGCTCGAAGAAATC+TGG | - | chr1.2:11907385-11907404 | None:intergenic | 40.0% | |
| TGGTAAAATCCCATTGGGAT+TGG | - | chr1.2:11906230-11906249 | None:intergenic | 40.0% | |
| TTAACACCACCGGATTACTA+GGG | - | chr1.2:11907044-11907063 | None:intergenic | 40.0% | |
| ! | AATCCCAATGGGATTTTACC+AGG | + | chr1.2:11906229-11906248 | MS.gene059165:CDS | 40.0% |
| !! | AGTGACTTGGAAGTGGATTT+TGG | + | chr1.2:11906590-11906609 | MS.gene059165:intron | 40.0% |
| !!! | TTGTTTTGGTTGCCAGAGTA+AGG | - | chr1.2:11907425-11907444 | None:intergenic | 40.0% |
| ACCACACAACCAATCCCAAT+GGG | + | chr1.2:11906218-11906237 | MS.gene059165:CDS | 45.0% | |
| CTTAACACCACCGGATTACT+AGG | - | chr1.2:11907045-11907064 | None:intergenic | 45.0% | |
| TGTATATGCTGCCTTAGCTG+TGG | + | chr1.2:11906634-11906653 | MS.gene059165:CDS | 45.0% | |
| ! | CAATTGCAGTGACTTGGAAG+TGG | + | chr1.2:11906583-11906602 | MS.gene059165:intron | 45.0% |
| !! | TAAATTGAATTATTAATTAA+TGG | - | chr1.2:11906426-11906445 | None:intergenic | 5.0% |
| AAGAAGCCCTAGTAATCCGG+TGG | + | chr1.2:11907035-11907054 | MS.gene059165:intron | 50.0% | |
| CACCACACAACCAATCCCAA+TGG | + | chr1.2:11906217-11906236 | MS.gene059165:CDS | 50.0% | |
| CGACTCATGACGGTGTCTAA+TGG | + | chr1.2:11906776-11906795 | MS.gene059165:CDS | 50.0% | |
| !! | TCCCATTGGGATTGGTTGTG+TGG | - | chr1.2:11906222-11906241 | None:intergenic | 50.0% |
| !! | TTGGGATTGGTTGTGTGGTG+TGG | - | chr1.2:11906217-11906236 | None:intergenic | 50.0% |
| CACCTCCTCATCCACAGCTA+AGG | - | chr1.2:11906648-11906667 | None:intergenic | 55.0% | |
| TGCCTTAGCTGTGGATGAGG+AGG | + | chr1.2:11906643-11906662 | MS.gene059165:CDS | 55.0% | |
| TGCTGCCTTAGCTGTGGATG+AGG | + | chr1.2:11906640-11906659 | MS.gene059165:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 11906207 | 11907476 | 11906207 | ID=MS.gene059165 |
| chr1.2 | mRNA | 11906207 | 11907476 | 11906207 | ID=MS.gene059165.t1;Parent=MS.gene059165 |
| chr1.2 | exon | 11906207 | 11906250 | 11906207 | ID=MS.gene059165.t1.exon1;Parent=MS.gene059165.t1 |
| chr1.2 | CDS | 11906207 | 11906250 | 11906207 | ID=cds.MS.gene059165.t1;Parent=MS.gene059165.t1 |
| chr1.2 | exon | 11906592 | 11906664 | 11906592 | ID=MS.gene059165.t1.exon2;Parent=MS.gene059165.t1 |
| chr1.2 | CDS | 11906592 | 11906664 | 11906592 | ID=cds.MS.gene059165.t1;Parent=MS.gene059165.t1 |
| chr1.2 | exon | 11906755 | 11906810 | 11906755 | ID=MS.gene059165.t1.exon3;Parent=MS.gene059165.t1 |
| chr1.2 | CDS | 11906755 | 11906810 | 11906755 | ID=cds.MS.gene059165.t1;Parent=MS.gene059165.t1 |
| chr1.2 | exon | 11907362 | 11907476 | 11907362 | ID=MS.gene059165.t1.exon4;Parent=MS.gene059165.t1 |
| chr1.2 | CDS | 11907362 | 11907476 | 11907362 | ID=cds.MS.gene059165.t1;Parent=MS.gene059165.t1 |
| Gene Sequence |
| Protein sequence |
