AlfalfaGEDB | MS.gene43491

M. sativa cultivar XinJiangDaYe / MS.gene43491

KEGG: -
GO: -
PFAM: PF08991.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43491.t1	KEH32768.1	97.1	69	2	0	1	69	1	69	7.30E-31	142.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43491.t1	Q0VBY0	43.3	60	32	1	6	65	4	61	1.8e-06	52.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43491.t1	A0A072UUM7	97.1	69	2	0	1	69	1	69	5.2e-31	142.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene43491	0.814173	1.76E-51	-1.69E-46
MS.gene049653	MS.gene43491	0.812956	3.27E-51	-1.69E-46
MS.gene049756	MS.gene43491	0.820011	8.59E-53	-1.69E-46
MS.gene050277	MS.gene43491	0.877544	5.77E-69	-1.69E-46
MS.gene050303	MS.gene43491	0.837978	3.78E-57	-1.69E-46
MS.gene050320	MS.gene43491	0.801023	1.09E-48	-1.69E-46
MS.gene050815	MS.gene43491	0.805295	1.43E-49	-1.69E-46
MS.gene050987	MS.gene43491	0.809274	2.05E-50	-1.69E-46
MS.gene051241	MS.gene43491	0.82773	1.33E-54	-1.69E-46
MS.gene051243	MS.gene43491	0.843868	1.08E-58	-1.69E-46
MS.gene051245	MS.gene43491	0.847644	1.02E-59	-1.69E-46
MS.gene051349	MS.gene43491	0.823486	1.35E-53	-1.69E-46
MS.gene05134	MS.gene43491	0.830377	3.04E-55	-1.69E-46
MS.gene051451	MS.gene43491	0.816388	5.67E-52	-1.69E-46
MS.gene051633	MS.gene43491	0.844001	9.91E-59	-1.69E-46
MS.gene051903	MS.gene43491	0.810616	1.06E-50	-1.69E-46
MS.gene052176	MS.gene43491	0.800434	1.44E-48	-1.69E-46
MS.gene052286	MS.gene43491	0.870227	1.71E-66	-1.69E-46
MS.gene052302	MS.gene43491	0.828075	1.10E-54	-1.69E-46
MS.gene052309	MS.gene43491	-0.854447	1.22E-61	-1.69E-46
MS.gene052376	MS.gene43491	0.829274	5.64E-55	-1.69E-46
MS.gene052947	MS.gene43491	0.807915	4.00E-50	-1.69E-46
MS.gene053080	MS.gene43491	0.802313	5.95E-49	-1.69E-46
MS.gene053109	MS.gene43491	0.805303	1.42E-49	-1.69E-46
MS.gene053188	MS.gene43491	0.805515	1.29E-49	-1.69E-46
MS.gene053216	MS.gene43491	0.824875	6.37E-54	-1.69E-46
MS.gene054537	MS.gene43491	0.812809	3.52E-51	-1.69E-46
MS.gene054657	MS.gene43491	0.802756	4.82E-49	-1.69E-46
MS.gene05510	MS.gene43491	0.806126	9.57E-50	-1.69E-46
MS.gene055174	MS.gene43491	0.813468	2.52E-51	-1.69E-46
MS.gene055808	MS.gene43491	0.80203	6.81E-49	-1.69E-46
MS.gene056631	MS.gene43491	0.844498	7.28E-59	-1.69E-46
MS.gene056760	MS.gene43491	0.815786	7.73E-52	-1.69E-46
MS.gene057060	MS.gene43491	0.817012	4.11E-52	-1.69E-46
MS.gene057079	MS.gene43491	0.853462	2.35E-61	-1.69E-46
MS.gene057140	MS.gene43491	0.839666	1.38E-57	-1.69E-46
MS.gene057234	MS.gene43491	0.846562	2.01E-59	-1.69E-46
MS.gene057392	MS.gene43491	0.813304	2.74E-51	-1.69E-46
MS.gene057555	MS.gene43491	0.820425	6.91E-53	-1.69E-46
MS.gene057705	MS.gene43491	0.811184	7.95E-51	-1.69E-46
MS.gene05795	MS.gene43491	0.844505	7.25E-59	-1.69E-46
MS.gene057999	MS.gene43491	0.800662	1.30E-48	-1.69E-46
MS.gene058055	MS.gene43491	0.812761	3.60E-51	-1.69E-46
MS.gene058182	MS.gene43491	0.800812	1.21E-48	-1.69E-46
MS.gene058238	MS.gene43491	0.851973	6.26E-61	-1.69E-46
MS.gene058399	MS.gene43491	0.810014	1.42E-50	-1.69E-46
MS.gene058906	MS.gene43491	0.822611	2.16E-53	-1.69E-46
MS.gene059165	MS.gene43491	0.808839	2.54E-50	-1.69E-46
MS.gene059317	MS.gene43491	0.830153	3.45E-55	-1.69E-46
MS.gene05954	MS.gene43491	0.816561	5.19E-52	-1.69E-46
MS.gene059569	MS.gene43491	0.805823	1.11E-49	-1.69E-46
MS.gene059830	MS.gene43491	0.814738	1.32E-51	-1.69E-46
MS.gene059898	MS.gene43491	0.825521	4.48E-54	-1.69E-46
MS.gene06001	MS.gene43491	0.812212	4.75E-51	-1.69E-46
MS.gene060297	MS.gene43491	0.831817	1.35E-55	-1.69E-46
MS.gene060586	MS.gene43491	0.823991	1.03E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43491.t1	MTR_4g134900	97.101	69	2	0	1	69	1	69	1.24e-43	135

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43491.t1	AT1G09794	69.841	63	19	0	1	63	1	63	2.24e-27	94.0

Find 10 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAGCAATTCAATAACTTTA+AGG	0.154340	8.4:-77110	None:intergenic
CCTTAAAGTTATTGAATTGC+TGG	0.305156	8.4:+77110	MS.gene43491:CDS
TTGCGGGAGAAAGTTGTTCT+TGG	0.356092	8.4:-76181	None:intergenic
ACTCACTGTGCTTCGCTCTC+TGG	0.391788	8.4:+77165	MS.gene43491:CDS
CAACTACTTATCCAATTACT+TGG	0.432849	8.4:-77306	None:intergenic
CAAAGAGCCTTGCAAGAAAG+AGG	0.485000	8.4:+76134	MS.gene43491:CDS
TTACAAGCCTCTTTCTTGCA+AGG	0.522587	8.4:-76141	None:intergenic
AAGGTTTGAGAAAATGGGGC+AGG	0.531461	8.4:+76107	None:intergenic
GGAGAAAGTTGTTCTTGGAG+AGG	0.604134	8.4:-76176	None:intergenic
TGTCTGCAGATTTCAAGACT+CGG	0.613114	8.4:+77283	MS.gene43491:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTTTATAAGCAAATAT+TGG	+	chr8.4:76918-76937	MS.gene43491:intron	10.0%
!!	AAAATCCCTAATAATATAAT+TGG	+	chr8.4:76306-76325	MS.gene43491:intron	15.0%
!!	AATTATATTAACACTAACAT+AGG	-	chr8.4:76383-76402	None:intergenic	15.0%
!!	ATGTTAGTGTTAATATAATT+GGG	+	chr8.4:76383-76402	MS.gene43491:intron	15.0%
!!	TATGTTAGTGTTAATATAAT+TGG	+	chr8.4:76382-76401	MS.gene43491:intron	15.0%
!!	TCAATCCAATTATATTATTA+GGG	-	chr8.4:76314-76333	None:intergenic	15.0%
!!	TTCAATCCAATTATATTATT+AGG	-	chr8.4:76315-76334	None:intergenic	15.0%
!!	AAAATTCCAGTTTCTAATAT+TGG	-	chr8.4:76903-76922	None:intergenic	20.0%
!!	CAAAACAAATCAAATGAAAT+AGG	-	chr8.4:76349-76368	None:intergenic	20.0%
!!	TGACATAGTTAATTATAAGT+TGG	+	chr8.4:76456-76475	MS.gene43491:intron	20.0%
!!!	GTTTTTAATTAAAACAACCA+CGG	+	chr8.4:76644-76663	MS.gene43491:intron	20.0%
!!!	TTAATTTTTGGAATTGAACT+CGG	+	chr8.4:76957-76976	MS.gene43491:intron	20.0%
!!!	TTAGTGGTTATGTTAATTTT+TGG	+	chr8.4:76945-76964	MS.gene43491:intron	20.0%
!	AAGTTTCTTATAATACTGCT+AGG	+	chr8.4:77085-77104	MS.gene43491:intron	25.0%
!	AGTATAATTCATCACTTACT+TGG	-	chr8.4:77208-77227	None:intergenic	25.0%
!	TAAAGAATACTTGAACACTA+CGG	+	chr8.4:76722-76741	MS.gene43491:intron	25.0%
!	TATATCACAATCAAAATGAG+TGG	-	chr8.4:76282-76301	None:intergenic	25.0%
!	TATTTAATTAACAGTCTGAC+AGG	-	chr8.4:76434-76453	None:intergenic	25.0%
!!	AAACAAAAACATACTTTTGC+GGG	-	chr8.4:76200-76219	None:intergenic	25.0%
!!	AATGTTTTGTAAAGATAGCT+TGG	-	chr8.4:77030-77049	None:intergenic	25.0%
!!	CAAACAAAAACATACTTTTG+CGG	-	chr8.4:76201-76220	None:intergenic	25.0%
!!!	GTTTTCTAACTTTTCTAAGA+AGG	-	chr8.4:76592-76611	None:intergenic	25.0%
	AGCAAATATTGGTAAGTTAG+TGG	+	chr8.4:76929-76948	MS.gene43491:intron	30.0%
	AGTTAGAAAACTTTCTTCTG+CGG	+	chr8.4:76601-76620	MS.gene43491:intron	30.0%
	CAAAACAAAAATATGCAAGC+CGG	-	chr8.4:77265-77284	None:intergenic	30.0%
	CATTGTTGAAACAAAACTGT+TGG	+	chr8.4:77046-77065	MS.gene43491:intron	30.0%
	CCAGCAATTCAATAACTTTA+AGG	-	chr8.4:77113-77132	None:intergenic	30.0%
	CCTTAAAGTTATTGAATTGC+TGG	+	chr8.4:77110-77129	MS.gene43491:CDS	30.0%
!	TTTTGTAAAGATAGCTTGGT+TGG	-	chr8.4:77026-77045	None:intergenic	30.0%
	AAAACCAAACACCTTTCATG+AGG	-	chr8.4:76629-76648	None:intergenic	35.0%
	ACAACGCCAATATTAGAAAC+TGG	+	chr8.4:76894-76913	MS.gene43491:intron	35.0%
	CAAATGATGCAAATGTCAGA+AGG	+	chr8.4:76830-76849	MS.gene43491:intron	35.0%
	CTTCTGACATTTGCATCATT+TGG	-	chr8.4:76832-76851	None:intergenic	35.0%
	TATGCCGGAATAGAGTTTAA+TGG	-	chr8.4:76542-76561	None:intergenic	35.0%
	TCCACCATTAAACTCTATTC+CGG	+	chr8.4:76535-76554	MS.gene43491:intron	35.0%
	TTCAGATTCATATCGATGAC+CGG	+	chr8.4:77243-77262	MS.gene43491:intron	35.0%
!!!	TTTTGGAATTGAACTCGGAA+GGG	+	chr8.4:76962-76981	MS.gene43491:intron	35.0%
!!!	TTTTTGGAATTGAACTCGGA+AGG	+	chr8.4:76961-76980	MS.gene43491:intron	35.0%
	GCACAGTGGTATCATCTAAA+TGG	+	chr8.4:76854-76873	MS.gene43491:intron	40.0%
	TCTAAATGGCTATGCCTATG+CGG	+	chr8.4:76868-76887	MS.gene43491:intron	40.0%
	TGTCTGCAGATTTCAAGACT+CGG	+	chr8.4:77283-77302	MS.gene43491:intron	40.0%
	TTACAAGCCTCTTTCTTGCA+AGG	-	chr8.4:76144-76163	None:intergenic	40.0%
!!	ATAGCTTGGTTGGTGAGTTA+AGG	-	chr8.4:77016-77035	None:intergenic	40.0%
!!	TAGCTTGGTTGGTGAGTTAA+GGG	-	chr8.4:77015-77034	None:intergenic	40.0%
	AAAACTCTCTTCCTGGTGAC+TGG	+	chr8.4:76685-76704	MS.gene43491:intron	45.0%
	ACAGTCTGACAGGAATGAAG+AGG	-	chr8.4:76424-76443	None:intergenic	45.0%
	CAAAGAGCCTTGCAAGAAAG+AGG	+	chr8.4:76134-76153	MS.gene43491:CDS	45.0%
	GCCGGAATAGAGTTTAATGG+TGG	-	chr8.4:76539-76558	None:intergenic	45.0%
	GGAGAAAGTTGTTCTTGGAG+AGG	-	chr8.4:76179-76198	None:intergenic	45.0%
	GTGGATATGCAGAAGCCATT+GGG	-	chr8.4:76520-76539	None:intergenic	45.0%
	TTGCGGGAGAAAGTTGTTCT+TGG	-	chr8.4:76184-76203	None:intergenic	45.0%
!	AAATGTCAGAAGGTGCACAG+TGG	+	chr8.4:76840-76859	MS.gene43491:intron	45.0%
!	AAGGAGTGCGAAAACAGTCT+CGG	-	chr8.4:76573-76592	None:intergenic	45.0%
	ATTGGCGTTGTGTTCCGCAT+AGG	-	chr8.4:76885-76904	None:intergenic	50.0%
	GCAGAAGCCATTGGGATAGA+GGG	-	chr8.4:76512-76531	None:intergenic	50.0%
	GCGCTGAAAAACTCTCTTCC+TGG	+	chr8.4:76678-76697	MS.gene43491:intron	50.0%
	GGTGGATATGCAGAAGCCAT+TGG	-	chr8.4:76521-76540	None:intergenic	50.0%
	TGCAGAAGCCATTGGGATAG+AGG	-	chr8.4:76513-76532	None:intergenic	50.0%
	TGTAACGCCCTCTATCCCAA+TGG	+	chr8.4:76502-76521	MS.gene43491:intron	50.0%
	ACTCACTGTGCTTCGCTCTC+TGG	+	chr8.4:77165-77184	MS.gene43491:CDS	55.0%
	CTCTGATCTCACCAGTCACC+AGG	-	chr8.4:76699-76718	None:intergenic	55.0%
	GAGTGCGAAAACAGTCTCGG+AGG	-	chr8.4:76570-76589	None:intergenic	55.0%
	GCGAAAACAGTCTCGGAGGT+TGG	-	chr8.4:76566-76585	None:intergenic	55.0%
!	GTCTCGGAGGTTGGTTATGC+CGG	-	chr8.4:76557-76576	None:intergenic	55.0%
!!	GGCGCCTCATGAAAGGTGTT+TGG	+	chr8.4:76622-76641	MS.gene43491:intron	55.0%
	CAGCGCAACTGTGATGTCCG+TGG	-	chr8.4:76664-76683	None:intergenic	60.0%
	CTTCTGCGGCGCCTCATGAA+AGG	+	chr8.4:76615-76634	MS.gene43491:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	76120	77313	76120	ID=MS.gene43491
chr8.4	mRNA	76120	77313	76120	ID=MS.gene43491.t1;Parent=MS.gene43491
chr8.4	exon	76120	76205	76120	ID=MS.gene43491.t1.exon1;Parent=MS.gene43491.t1
chr8.4	CDS	76120	76205	76120	ID=cds.MS.gene43491.t1;Parent=MS.gene43491.t1
chr8.4	exon	77107	77208	77107	ID=MS.gene43491.t1.exon2;Parent=MS.gene43491.t1
chr8.4	CDS	77107	77208	77107	ID=cds.MS.gene43491.t1;Parent=MS.gene43491.t1
chr8.4	exon	77292	77313	77292	ID=MS.gene43491.t1.exon3;Parent=MS.gene43491.t1
chr8.4	CDS	77292	77313	77292	ID=cds.MS.gene43491.t1;Parent=MS.gene43491.t1

Gene Sequence

>MS.gene43491

ATGGGGCAGGAAAGCAAAGAGCCTTGCAAGAAAGAGGCTTGTAACATTCAAGCTTGCCTCTCCAAGAACAACTTTCTCCCGCAAAAGTATGTTTTTGTTTGTTTCGATTTTATTTATTTAATTTCTGTTTGCATATAGTGATTTGATTGAGAAATCGCTCCACTCATTTTGATTGTGATATATTGAAAAATCCCTAATAATATAATTGGATTGAAGATGTTGATTACCTATTTCATTTGATTTGTTTTGATTTAATTTCTCCTATGTTAGTGTTAATATAATTGGGTTAGAAGATCAATTACCTCTTCATTCCTGTCAGACTGTTAATTAAATAACTGACATAGTTAATTATAAGTTGGAACTAATCAATCTCTTACTGAATTGTAACGCCCTCTATCCCAATGGCTTCTGCATATCCACCATTAAACTCTATTCCGGCATAACCAACCTCCGAGACTGTTTTCGCACTCCTTCTTAGAAAAGTTAGAAAACTTTCTTCTGCGGCGCCTCATGAAAGGTGTTTGGTTTTTAATTAAAACAACCACGGACATCACAGTTGCGCTGAAAAACTCTCTTCCTGGTGACTGGTGAGATCAGAGAGATAAAGAATACTTGAACACTACGGAAGATACTTTTATCATAGAAGTACTAATTTATGTCAATTACTATATAGCACAACTACTGTGAATCTAATTCAATAAGACTGTTACCAAATGATGCAAATGTCAGAAGGTGCACAGTGGTATCATCTAAATGGCTATGCCTATGCGGAACACAACGCCAATATTAGAAACTGGAATTTTTTTATAAGCAAATATTGGTAAGTTAGTGGTTATGTTAATTTTTGGAATTGAACTCGGAAGGGATCTCGTGCTTAAAACTTCTTGAGTGTCCCTTAACTCACCAACCAAGCTATCTTTACAAAACATTGTTGAAACAAAACTGTTGGACTGAGTTGCTGTCTGAAGTTTCTTATAATACTGCTAGGTGCCTTAAAGTTATTGAATTGCTGGACTCTTGCTGCTCAAGATGCGATTATAATTCAACTCACTGTGCTTCGCTCTCTGGACTATTGAAGCAAAATTCCAAGTAAGTGATGAATTATACTTTCATTTTTCACTATTTCAGATTCATATCGATGACCGGCTTGCATATTTTTGTTTTGTCTGCAGATTTCAAGACTCGGCCAAGTAA

Protein sequence

>MS.gene43491.t1

MGQESKEPCKKEACNIQACLSKNNFLPQKCLKVIELLDSCCSRCDYNSTHCASLSGLLKQNSKFQDSAK