AlfalfaGEDB | MS.gene95039

M. sativa cultivar XinJiangDaYe / MS.gene95039

KEGG: -
GO: -
PFAM: PF08991.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95039.t1	KEH32768.1	97.1	69	2	0	1	69	1	69	7.30E-31	142.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95039.t1	Q0VBY0	43.3	60	32	1	6	65	4	61	1.8e-06	52.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95039.t1	A0A072UUM7	97.1	69	2	0	1	69	1	69	5.2e-31	142.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene95039	0.816834	4.51E-52	-1.69E-46
MS.gene049653	MS.gene95039	0.810006	1.43E-50	-1.69E-46
MS.gene049756	MS.gene95039	0.814328	1.63E-51	-1.69E-46
MS.gene050277	MS.gene95039	0.8725	3.03E-67	-1.69E-46
MS.gene050303	MS.gene95039	0.833808	4.31E-56	-1.69E-46
MS.gene050320	MS.gene95039	0.800559	1.36E-48	-1.69E-46
MS.gene050987	MS.gene95039	0.808913	2.45E-50	-1.69E-46
MS.gene051241	MS.gene95039	0.833225	6.03E-56	-1.69E-46
MS.gene051243	MS.gene95039	0.846539	2.04E-59	-1.69E-46
MS.gene051245	MS.gene95039	0.839103	1.94E-57	-1.69E-46
MS.gene051349	MS.gene95039	0.821108	4.81E-53	-1.69E-46
MS.gene05134	MS.gene95039	0.833642	4.74E-56	-1.69E-46
MS.gene051451	MS.gene95039	0.814501	1.49E-51	-1.69E-46
MS.gene051633	MS.gene95039	0.835544	1.58E-56	-1.69E-46
MS.gene051903	MS.gene95039	0.806268	8.93E-50	-1.69E-46
MS.gene052286	MS.gene95039	0.868338	7.03E-66	-1.69E-46
MS.gene052302	MS.gene95039	0.825153	5.48E-54	-1.69E-46
MS.gene052309	MS.gene95039	-0.847517	1.10E-59	-1.69E-46
MS.gene052376	MS.gene95039	0.826703	2.35E-54	-1.69E-46
MS.gene052947	MS.gene95039	0.806009	1.01E-49	-1.69E-46
MS.gene053080	MS.gene95039	0.805814	1.11E-49	-1.69E-46
MS.gene053109	MS.gene95039	0.802856	4.60E-49	-1.69E-46
MS.gene053188	MS.gene95039	0.804798	1.82E-49	-1.69E-46
MS.gene053216	MS.gene95039	0.820231	7.65E-53	-1.69E-46
MS.gene054537	MS.gene95039	0.811348	7.32E-51	-1.69E-46
MS.gene054657	MS.gene95039	0.803022	4.25E-49	-1.69E-46
MS.gene05510	MS.gene95039	0.803816	2.91E-49	-1.69E-46
MS.gene055174	MS.gene95039	0.806626	7.51E-50	-1.69E-46
MS.gene055808	MS.gene95039	0.80344	3.48E-49	-1.69E-46
MS.gene056631	MS.gene95039	0.841282	5.22E-58	-1.69E-46
MS.gene056760	MS.gene95039	0.811465	6.91E-51	-1.69E-46
MS.gene057060	MS.gene95039	0.822441	2.36E-53	-1.69E-46
MS.gene057079	MS.gene95039	0.853535	2.24E-61	-1.69E-46
MS.gene057140	MS.gene95039	0.837919	3.91E-57	-1.69E-46
MS.gene057234	MS.gene95039	0.840151	1.03E-57	-1.69E-46
MS.gene057392	MS.gene95039	0.815466	9.11E-52	-1.69E-46
MS.gene057555	MS.gene95039	0.819355	1.21E-52	-1.69E-46
MS.gene057705	MS.gene95039	0.818573	1.83E-52	-1.69E-46
MS.gene05795	MS.gene95039	0.846925	1.60E-59	-1.69E-46
MS.gene058055	MS.gene95039	0.812181	4.82E-51	-1.69E-46
MS.gene058238	MS.gene95039	0.844194	8.79E-59	-1.69E-46
MS.gene058399	MS.gene95039	0.805629	1.22E-49	-1.69E-46
MS.gene058906	MS.gene95039	0.814205	1.73E-51	-1.69E-46
MS.gene059165	MS.gene95039	0.809916	1.49E-50	-1.69E-46
MS.gene059317	MS.gene95039	0.82906	6.36E-55	-1.69E-46
MS.gene05954	MS.gene95039	0.821192	4.60E-53	-1.69E-46
MS.gene059569	MS.gene95039	0.80918	2.15E-50	-1.69E-46
MS.gene059830	MS.gene95039	0.810345	1.21E-50	-1.69E-46
MS.gene059898	MS.gene95039	0.81914	1.36E-52	-1.69E-46
MS.gene06001	MS.gene95039	0.807874	4.08E-50	-1.69E-46
MS.gene060297	MS.gene95039	0.821959	3.06E-53	-1.69E-46
MS.gene060586	MS.gene95039	0.820807	5.64E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95039.t1	MTR_4g134900	97.101	69	2	0	1	69	1	69	1.24e-43	135

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95039.t1	AT1G09794	69.841	63	19	0	1	63	1	63	2.24e-27	94.0

Find 10 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAGCAATTCAATAACTTTA+AGG	0.154340	8.2:-91593	None:intergenic
CCTTAAAGTTATTGAATTGC+TGG	0.305156	8.2:+91593	MS.gene95039:CDS
TTGCGGGAGAAAGTTGTTCT+TGG	0.356092	8.2:-90660	None:intergenic
ACTCACTGTGCTTCGCTCTC+TGG	0.391788	8.2:+91648	MS.gene95039:CDS
CAACTACTTATCCAATTACT+TGG	0.432849	8.2:-91789	None:intergenic
CAAAGAGCCTTGCAAGAAAG+AGG	0.485000	8.2:+90613	MS.gene95039:CDS
TTACAAGCCTCTTTCTTGCA+AGG	0.522587	8.2:-90620	None:intergenic
AAGGTTTGAGAAAATGGGGC+AGG	0.531461	8.2:+90586	None:intergenic
GGAGAAAGTTGTTCTTGGAG+AGG	0.604134	8.2:-90655	None:intergenic
TGTCTGCAGATTTCAAGACT+CGG	0.613114	8.2:+91766	MS.gene95039:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTTTATAAGCAAATAT+TGG	+	chr8.2:91401-91420	MS.gene95039:intron	10.0%
!!	AAAATCCCTAATAATATAAT+TGG	+	chr8.2:90785-90804	MS.gene95039:intron	15.0%
!!	AATTATATTAACACTAACAT+AGG	-	chr8.2:90862-90881	None:intergenic	15.0%
!!	ATGTTAGTGTTAATATAATT+GGG	+	chr8.2:90862-90881	MS.gene95039:intron	15.0%
!!	TATGTTAGTGTTAATATAAT+TGG	+	chr8.2:90861-90880	MS.gene95039:intron	15.0%
!!	TCAATCCAATTATATTATTA+GGG	-	chr8.2:90793-90812	None:intergenic	15.0%
!!	TTCAATCCAATTATATTATT+AGG	-	chr8.2:90794-90813	None:intergenic	15.0%
!!!	TTAATGGTTATGTTAATTTT+TGG	+	chr8.2:91428-91447	MS.gene95039:intron	15.0%
!!	AAAATTCCAGTTTCTAATAT+TGG	-	chr8.2:91386-91405	None:intergenic	20.0%
!!	CAAAACAAATCAAATGAAAT+AGG	-	chr8.2:90828-90847	None:intergenic	20.0%
!!	TGACATAGTTAATTATAAGT+TGG	+	chr8.2:90935-90954	MS.gene95039:intron	20.0%
!!!	GTTTTTAATTAAAACAACCA+CGG	+	chr8.2:91127-91146	MS.gene95039:intron	20.0%
!!!	TTAATTTTTGGAATTGAACT+CGG	+	chr8.2:91440-91459	MS.gene95039:intron	20.0%
!	AAGTTTCTTATAATACTGCT+AGG	+	chr8.2:91568-91587	MS.gene95039:intron	25.0%
!	AGCAAATATTGGTAAGTTAA+TGG	+	chr8.2:91412-91431	MS.gene95039:intron	25.0%
!	AGTATAATTCATCACTTACT+TGG	-	chr8.2:91691-91710	None:intergenic	25.0%
!	TAAAGAATACTTGAACACTA+CGG	+	chr8.2:91205-91224	MS.gene95039:intron	25.0%
!	TATATCACAATCAAAATCAG+TGG	-	chr8.2:90761-90780	None:intergenic	25.0%
!	TATTTAATTAACAGTCTGAC+AGG	-	chr8.2:90913-90932	None:intergenic	25.0%
!!	AAACAAAAACATACTTTTGC+GGG	-	chr8.2:90679-90698	None:intergenic	25.0%
!!	AATGTTTTGTAAAGATAGCT+TGG	-	chr8.2:91513-91532	None:intergenic	25.0%
!!	CAAACAAAAACATACTTTTG+CGG	-	chr8.2:90680-90699	None:intergenic	25.0%
!!!	GTTTTCTAACTTTTCTAAGA+AGG	-	chr8.2:91071-91090	None:intergenic	25.0%
	AGTTAGAAAACTTTCTTCTG+CGG	+	chr8.2:91080-91099	MS.gene95039:intron	30.0%
	CAAAACAAAAATATGCAAGC+CGG	-	chr8.2:91748-91767	None:intergenic	30.0%
	CATTGTTGAAACAAAACTGT+TGG	+	chr8.2:91529-91548	MS.gene95039:intron	30.0%
	CCAGCAATTCAATAACTTTA+AGG	-	chr8.2:91596-91615	None:intergenic	30.0%
	CCTTAAAGTTATTGAATTGC+TGG	+	chr8.2:91593-91612	MS.gene95039:CDS	30.0%
!	TTTTGTAAAGATAGCTTGGT+TGG	-	chr8.2:91509-91528	None:intergenic	30.0%
	ACAACGCCAATATTAGAAAC+TGG	+	chr8.2:91377-91396	MS.gene95039:intron	35.0%
	CAAATGATGCAAATGTCAGA+AGG	+	chr8.2:91313-91332	MS.gene95039:intron	35.0%
	CTTCTGACATTTGCATCATT+TGG	-	chr8.2:91315-91334	None:intergenic	35.0%
	TATGCCGGAATAGAGTTTAA+TGG	-	chr8.2:91021-91040	None:intergenic	35.0%
	TCCACCATTAAACTCTATTC+CGG	+	chr8.2:91014-91033	MS.gene95039:intron	35.0%
	TTCAGATTCATATCGATGAC+CGG	+	chr8.2:91726-91745	MS.gene95039:intron	35.0%
!!!	TTTTGGAATTGAACTCGGAA+GGG	+	chr8.2:91445-91464	MS.gene95039:intron	35.0%
!!!	TTTTTGGAATTGAACTCGGA+AGG	+	chr8.2:91444-91463	MS.gene95039:intron	35.0%
	GCACAGTGGTATCATCTAAA+TGG	+	chr8.2:91337-91356	MS.gene95039:intron	40.0%
	TCTAAATGGCTATGCCTATG+CGG	+	chr8.2:91351-91370	MS.gene95039:intron	40.0%
	TGTCTGCAGATTTCAAGACT+CGG	+	chr8.2:91766-91785	MS.gene95039:intron	40.0%
	TTACAAGCCTCTTTCTTGCA+AGG	-	chr8.2:90623-90642	None:intergenic	40.0%
!!	ATAGCTTGGTTGGTGAGTTA+AGG	-	chr8.2:91499-91518	None:intergenic	40.0%
!!	TAGCTTGGTTGGTGAGTTAA+GGG	-	chr8.2:91498-91517	None:intergenic	40.0%
	AAAACTCTCTTCCTGGTGAC+TGG	+	chr8.2:91168-91187	MS.gene95039:intron	45.0%
	ACAGTCTGACAGGAATGAAG+AGG	-	chr8.2:90903-90922	None:intergenic	45.0%
	CAAAGAGCCTTGCAAGAAAG+AGG	+	chr8.2:90613-90632	MS.gene95039:CDS	45.0%
	CCTACCAAACACCTTTCATG+AGG	-	chr8.2:91108-91127	None:intergenic	45.0%
	GCCGGAATAGAGTTTAATGG+TGG	-	chr8.2:91018-91037	None:intergenic	45.0%
	GGAGAAAGTTGTTCTTGGAG+AGG	-	chr8.2:90658-90677	None:intergenic	45.0%
	GTGGATATGCAGAAGCCATT+GGG	-	chr8.2:90999-91018	None:intergenic	45.0%
	TTGCGGGAGAAAGTTGTTCT+TGG	-	chr8.2:90663-90682	None:intergenic	45.0%
!	AAATGTCAGAAGGTGCACAG+TGG	+	chr8.2:91323-91342	MS.gene95039:intron	45.0%
!	AAGGAGTGCGAAAACAGTCT+CGG	-	chr8.2:91052-91071	None:intergenic	45.0%
!!	CCTCATGAAAGGTGTTTGGT+AGG	+	chr8.2:91105-91124	MS.gene95039:intron	45.0%
	ATTGGCGTTGTGTTCCGCAT+AGG	-	chr8.2:91368-91387	None:intergenic	50.0%
	GCGCTGAAAAACTCTCTTCC+TGG	+	chr8.2:91161-91180	MS.gene95039:intron	50.0%
	GGTGGATATGCAGAAGCCAT+TGG	-	chr8.2:91000-91019	None:intergenic	50.0%
	ACTCACTGTGCTTCGCTCTC+TGG	+	chr8.2:91648-91667	MS.gene95039:CDS	55.0%
	CTCTGATCTCACCAGTCACC+AGG	-	chr8.2:91182-91201	None:intergenic	55.0%
	GAGTGCGAAAACAGTCTCGG+AGG	-	chr8.2:91049-91068	None:intergenic	55.0%
	GCAGAAGCCATTGGGACAGA+GGG	-	chr8.2:90991-91010	None:intergenic	55.0%
	GCGAAAACAGTCTCGGAGGT+TGG	-	chr8.2:91045-91064	None:intergenic	55.0%
	TGCAGAAGCCATTGGGACAG+AGG	-	chr8.2:90992-91011	None:intergenic	55.0%
	TGTAACGCCCTCTGTCCCAA+TGG	+	chr8.2:90981-91000	MS.gene95039:intron	55.0%
!	GTCTCGGAGGTTGGTTATGC+CGG	-	chr8.2:91036-91055	None:intergenic	55.0%
!!	GGCGCCTCATGAAAGGTGTT+TGG	+	chr8.2:91101-91120	MS.gene95039:intron	55.0%
	CAGCGCAACTGTGATGTCCG+TGG	-	chr8.2:91147-91166	None:intergenic	60.0%
	CTTCTGCGGCGCCTCATGAA+AGG	+	chr8.2:91094-91113	MS.gene95039:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	90599	91796	90599	ID=MS.gene95039
chr8.2	mRNA	90599	91796	90599	ID=MS.gene95039.t1;Parent=MS.gene95039
chr8.2	exon	90599	90684	90599	ID=MS.gene95039.t1.exon1;Parent=MS.gene95039.t1
chr8.2	CDS	90599	90684	90599	ID=cds.MS.gene95039.t1;Parent=MS.gene95039.t1
chr8.2	exon	91590	91691	91590	ID=MS.gene95039.t1.exon2;Parent=MS.gene95039.t1
chr8.2	CDS	91590	91691	91590	ID=cds.MS.gene95039.t1;Parent=MS.gene95039.t1
chr8.2	exon	91775	91796	91775	ID=MS.gene95039.t1.exon3;Parent=MS.gene95039.t1
chr8.2	CDS	91775	91796	91775	ID=cds.MS.gene95039.t1;Parent=MS.gene95039.t1

Gene Sequence

>MS.gene95039

ATGGGGCAGGAAAGCAAAGAGCCTTGCAAGAAAGAGGCTTGTAACATTCAAGCTTGCCTCTCCAAGAACAACTTTCTCCCGCAAAAGTATGTTTTTGTTTGTTTCGATTTTATTTATTTAATTTCTGTTTGCATATAGTGATTTGATTGAGAAATCGCTCCACTGATTTTGATTGTGATATATTGAAAAATCCCTAATAATATAATTGGATTGAAGATGTTGATTACCTATTTCATTTGATTTGTTTTGATTTAATTTCTCCTATGTTAGTGTTAATATAATTGGGTTAGAAGATCAATTACCTCTTCATTCCTGTCAGACTGTTAATTAAATAACTGACATAGTTAATTATAAGTTGGAACTAATCAATCTCTTACTGAATTGTAACGCCCTCTGTCCCAATGGCTTCTGCATATCCACCATTAAACTCTATTCCGGCATAACCAACCTCCGAGACTGTTTTCGCACTCCTTCTTAGAAAAGTTAGAAAACTTTCTTCTGCGGCGCCTCATGAAAGGTGTTTGGTAGGTTTTTAATTAAAACAACCACGGACATCACAGTTGCGCTGAAAAACTCTCTTCCTGGTGACTGGTGAGATCAGAGAGATAAAGAATACTTGAACACTACGGAAGATACTTTTATCATAGAAGTACTAATTTATGTCAATTACTATATAGCACAACTACTGTGAATCTAATTCAATAAGACTGTTACCAAATGATGCAAATGTCAGAAGGTGCACAGTGGTATCATCTAAATGGCTATGCCTATGCGGAACACAACGCCAATATTAGAAACTGGAATTTTTTTATAAGCAAATATTGGTAAGTTAATGGTTATGTTAATTTTTGGAATTGAACTCGGAAGGGATCTCGTGCTTAAAACTTCTTGAGTGTCCCTTAACTCACCAACCAAGCTATCTTTACAAAACATTGTTGAAACAAAACTGTTGGACTGAGTTGCTGTCTGAAGTTTCTTATAATACTGCTAGGTGCCTTAAAGTTATTGAATTGCTGGACTCTTGCTGCTCAAGATGCGATTATAATTCAACTCACTGTGCTTCGCTCTCTGGACTATTGAAGCAAAATTCCAAGTAAGTGATGAATTATACTTTCATTTTTCACTATTTCAGATTCATATCGATGACCGGCTTGCATATTTTTGTTTTGTCTGCAGATTTCAAGACTCGGCCAAGTAA

Protein sequence

>MS.gene95039.t1

MGQESKEPCKKEACNIQACLSKNNFLPQKCLKVIELLDSCCSRCDYNSTHCASLSGLLKQNSKFQDSAK