Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059605.t1 | RHN52951.1 | 78.8 | 259 | 54 | 1 | 10 | 268 | 4 | 261 | 1.00E-107 | 399.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059605.t1 | A0A396HKG4 | 78.8 | 259 | 54 | 1 | 10 | 268 | 4 | 261 | 7.2e-108 | 399.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05686 | MS.gene059605 | 0.810308 | 1.23E-50 | -1.69E-46 |
| MS.gene059605 | MS.gene068308 | 0.804834 | 1.79E-49 | -1.69E-46 |
| MS.gene059605 | MS.gene28530 | 0.811975 | 5.35E-51 | -1.69E-46 |
| MS.gene059605 | MS.gene29009 | 0.800435 | 1.44E-48 | -1.69E-46 |
| MS.gene059605 | MS.gene36151 | 0.812635 | 3.84E-51 | -1.69E-46 |
| MS.gene059605 | MS.gene44972 | 0.800758 | 1.24E-48 | -1.69E-46 |
| MS.gene059605 | MS.gene50875 | 0.802488 | 5.48E-49 | -1.69E-46 |
| MS.gene059605 | MS.gene51189 | 0.824234 | 9.01E-54 | -1.69E-46 |
| MS.gene059605 | MS.gene53827 | 0.809946 | 1.47E-50 | -1.69E-46 |
| MS.gene059605 | MS.gene66192 | 0.81056 | 1.08E-50 | -1.69E-46 |
| MS.gene059605 | MS.gene76954 | 0.808474 | 3.04E-50 | -1.69E-46 |
| MS.gene059605 | MS.gene81783 | 0.802862 | 4.59E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059605.t1 | MTR_6g488240 | 80.498 | 241 | 46 | 1 | 9 | 249 | 2 | 241 | 7.76e-141 | 398 |
| MS.gene059605.t1 | MTR_6g488220 | 43.348 | 233 | 118 | 7 | 1 | 224 | 1 | 228 | 2.26e-42 | 147 |
| MS.gene059605.t1 | MTR_6g488260 | 34.978 | 223 | 134 | 6 | 1 | 215 | 1 | 220 | 4.08e-28 | 109 |
| MS.gene059605.t1 | MTR_6g488180 | 45.926 | 135 | 49 | 5 | 70 | 191 | 51 | 174 | 1.23e-23 | 97.4 |
| MS.gene059605.t1 | MTR_6g488200 | 35.393 | 178 | 99 | 6 | 101 | 268 | 124 | 295 | 9.36e-21 | 89.7 |
| MS.gene059605.t1 | MTR_6g488190 | 35.878 | 131 | 82 | 2 | 103 | 232 | 92 | 221 | 7.19e-17 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTGAAGCAATACTAAATA+AGG | 0.225504 | 6.4:-60953419 | None:intergenic |
| TCACGAAGATGGTCTCATTA+AGG | 0.371609 | 6.4:+60952998 | MS.gene059605:CDS |
| TCCTATAGTCACACCATGAT+TGG | 0.372448 | 6.4:-60953585 | None:intergenic |
| ACTAAATAAGGGTGTTCATA+AGG | 0.398064 | 6.4:-60953407 | None:intergenic |
| GGAGAATAATGGTGAAGAAA+TGG | 0.398201 | 6.4:+60953529 | MS.gene059605:CDS |
| AAGTATCGAGAACATGGATT+TGG | 0.409114 | 6.4:+60953450 | MS.gene059605:CDS |
| TGAGGTTGGACTTTCAATAA+TGG | 0.411076 | 6.4:+60953337 | MS.gene059605:CDS |
| TCCAATCATGGTGTGACTAT+AGG | 0.424017 | 6.4:+60953584 | MS.gene059605:CDS |
| TGATTTGAATGTTTCAATGA+AGG | 0.429012 | 6.4:+60953382 | MS.gene059605:CDS |
| ATATGGACATCCTAGTTTGT+TGG | 0.452148 | 6.4:-60953662 | None:intergenic |
| TAATGAGACTAAGAACCATT+GGG | 0.460274 | 6.4:+60953151 | MS.gene059605:CDS |
| CAATTTGAGACGGAGAATAA+TGG | 0.462371 | 6.4:+60953518 | MS.gene059605:CDS |
| GTGAAGAAATGGAACGGTCT+AGG | 0.464145 | 6.4:+60953540 | MS.gene059605:CDS |
| TAATAATATGCCAACAAACT+AGG | 0.470313 | 6.4:+60953652 | MS.gene059605:CDS |
| ATGAGTTTGAGCAGCACAAT+GGG | 0.472444 | 6.4:-60953489 | None:intergenic |
| TATAATGAAGAATGTAAATA+CGG | 0.476092 | 6.4:-60953054 | None:intergenic |
| TTTGAAGCAATACTAAATAA+GGG | 0.484532 | 6.4:-60953418 | None:intergenic |
| GGAAATAGTATTTCCAATCA+TGG | 0.484591 | 6.4:+60953572 | MS.gene059605:CDS |
| ACACTACGTAGATGGTAAAT+CGG | 0.499784 | 6.4:+60952947 | MS.gene059605:CDS |
| ACAGTGTCACACTACGTAGA+TGG | 0.500726 | 6.4:+60952939 | MS.gene059605:CDS |
| ATAATGAGACTAAGAACCAT+TGG | 0.500792 | 6.4:+60953150 | MS.gene059605:CDS |
| GTTCCCACGCTCTTCTCCGA+TGG | 0.501338 | 6.4:-60952864 | None:intergenic |
| TTGGGGAGGGATTACGAAAA+CGG | 0.508005 | 6.4:+60953169 | MS.gene059605:CDS |
| GTGACTATAGGAGATTATAA+TGG | 0.514750 | 6.4:+60953596 | MS.gene059605:CDS |
| CGAGAACATGGATTTGGAAG+TGG | 0.516160 | 6.4:+60953456 | MS.gene059605:CDS |
| CGCAGTTTCAGTAGAGAGGA+AGG | 0.521608 | 6.4:+60952911 | MS.gene059605:CDS |
| TTGATAATATATCACGAAGA+TGG | 0.534894 | 6.4:+60952987 | MS.gene059605:CDS |
| GTGCAGCAGAAGAGAAAGAA+GGG | 0.548287 | 6.4:+60953236 | MS.gene059605:CDS |
| TGTTTCATAAAGTGAATCGA+TGG | 0.552717 | 6.4:-60953120 | None:intergenic |
| TTCAACAAGTATCGAGAACA+TGG | 0.554270 | 6.4:+60953444 | MS.gene059605:CDS |
| GTTTCATAAAGTGAATCGAT+GGG | 0.554819 | 6.4:-60953119 | None:intergenic |
| AACACGCAGTTTCAGTAGAG+AGG | 0.566105 | 6.4:+60952907 | MS.gene059605:CDS |
| GGTGCAGCAGAAGAGAAAGA+AGG | 0.574995 | 6.4:+60953235 | MS.gene059605:CDS |
| AATGTAAATACGGATCCAGT+AGG | 0.609266 | 6.4:-60953044 | None:intergenic |
| TTTCCATCGGAGAAGAGCGT+GGG | 0.612805 | 6.4:+60952861 | MS.gene059605:CDS |
| TATGAGTTTGAGCAGCACAA+TGG | 0.617125 | 6.4:-60953490 | None:intergenic |
| AGACTAAGAACCATTGGGGA+GGG | 0.620733 | 6.4:+60953156 | MS.gene059605:CDS |
| AAAACGGATGTTGCATACCG+TGG | 0.641753 | 6.4:+60953185 | MS.gene059605:CDS |
| TCATATGCAACAATTTGAGA+CGG | 0.647161 | 6.4:+60953508 | MS.gene059605:CDS |
| GAGACTAAGAACCATTGGGG+AGG | 0.649630 | 6.4:+60953155 | MS.gene059605:CDS |
| AAATGATGGGAATCTTCTTG+TGG | 0.669532 | 6.4:+60953214 | MS.gene059605:CDS |
| ATAATGGTGAAGAAATGGAA+CGG | 0.671072 | 6.4:+60953534 | MS.gene059605:CDS |
| GAACGGTCTAGGCAAAGCAG+AGG | 0.683040 | 6.4:+60953551 | MS.gene059605:CDS |
| AATGAGACTAAGAACCATTG+GGG | 0.684064 | 6.4:+60953152 | MS.gene059605:CDS |
| TGTGCCACTCTGATTGCACA+AGG | 0.692862 | 6.4:-60953269 | None:intergenic |
| AAAGCCTTGTGCAATCAGAG+TGG | 0.727260 | 6.4:+60953265 | MS.gene059605:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAATGAAGAATGTAAATA+CGG | - | chr6.4:60953057-60953076 | None:intergenic | 15.0% |
| !!! | ATTTGAAGCAATACTAAATA+AGG | - | chr6.4:60953422-60953441 | None:intergenic | 20.0% |
| !!! | TTTGAAGCAATACTAAATAA+GGG | - | chr6.4:60953421-60953440 | None:intergenic | 20.0% |
| ! | TGATTTGAATGTTTCAATGA+AGG | + | chr6.4:60953382-60953401 | MS.gene059605:CDS | 25.0% |
| !! | GACTTAAATTTCGTTTTCTA+CGG | + | chr6.4:60953020-60953039 | MS.gene059605:CDS | 25.0% |
| !! | TTGATAATATATCACGAAGA+TGG | + | chr6.4:60952987-60953006 | MS.gene059605:CDS | 25.0% |
| !!! | GTAAACAATGCTTTTTAAGT+CGG | + | chr6.4:60953306-60953325 | MS.gene059605:CDS | 25.0% |
| ATAATGAGACTAAGAACCAT+TGG | + | chr6.4:60953150-60953169 | MS.gene059605:CDS | 30.0% | |
| ATAATGGTGAAGAAATGGAA+CGG | + | chr6.4:60953534-60953553 | MS.gene059605:CDS | 30.0% | |
| GGAAATAGTATTTCCAATCA+TGG | + | chr6.4:60953572-60953591 | MS.gene059605:CDS | 30.0% | |
| GTGACTATAGGAGATTATAA+TGG | + | chr6.4:60953596-60953615 | MS.gene059605:CDS | 30.0% | |
| GTTTCATAAAGTGAATCGAT+GGG | - | chr6.4:60953122-60953141 | None:intergenic | 30.0% | |
| TAATGAGACTAAGAACCATT+GGG | + | chr6.4:60953151-60953170 | MS.gene059605:CDS | 30.0% | |
| TCATATGCAACAATTTGAGA+CGG | + | chr6.4:60953508-60953527 | MS.gene059605:CDS | 30.0% | |
| TGTTTCATAAAGTGAATCGA+TGG | - | chr6.4:60953123-60953142 | None:intergenic | 30.0% | |
| TTTAAGTCGGAAAAAAAGTG+AGG | + | chr6.4:60953319-60953338 | MS.gene059605:CDS | 30.0% | |
| !! | ACTAAATAAGGGTGTTCATA+AGG | - | chr6.4:60953410-60953429 | None:intergenic | 30.0% |
| AATGAGACTAAGAACCATTG+GGG | + | chr6.4:60953152-60953171 | MS.gene059605:CDS | 35.0% | |
| AATGTAAATACGGATCCAGT+AGG | - | chr6.4:60953047-60953066 | None:intergenic | 35.0% | |
| ACACTACGTAGATGGTAAAT+CGG | + | chr6.4:60952947-60952966 | MS.gene059605:CDS | 35.0% | |
| ACCGTGGAGATAAAAATGAT+GGG | + | chr6.4:60953201-60953220 | MS.gene059605:CDS | 35.0% | |
| GGAGAATAATGGTGAAGAAA+TGG | + | chr6.4:60953529-60953548 | MS.gene059605:CDS | 35.0% | |
| TACCGTGGAGATAAAAATGA+TGG | + | chr6.4:60953200-60953219 | MS.gene059605:CDS | 35.0% | |
| TGAGGTTGGACTTTCAATAA+TGG | + | chr6.4:60953337-60953356 | MS.gene059605:CDS | 35.0% | |
| ! | AAATGATGGGAATCTTCTTG+TGG | + | chr6.4:60953214-60953233 | MS.gene059605:CDS | 35.0% |
| ! | CAATTTGAGACGGAGAATAA+TGG | + | chr6.4:60953518-60953537 | MS.gene059605:CDS | 35.0% |
| !! | AAGTATCGAGAACATGGATT+TGG | + | chr6.4:60953450-60953469 | MS.gene059605:CDS | 35.0% |
| !! | TCCCATCATTTTTATCTCCA+CGG | - | chr6.4:60953205-60953224 | None:intergenic | 35.0% |
| !! | TTCAACAAGTATCGAGAACA+TGG | + | chr6.4:60953444-60953463 | MS.gene059605:CDS | 35.0% |
| AGTCGGAAAAAAAGTGAGGT+TGG | + | chr6.4:60953323-60953342 | MS.gene059605:CDS | 40.0% | |
| ATGAGTTTGAGCAGCACAAT+GGG | - | chr6.4:60953492-60953511 | None:intergenic | 40.0% | |
| TATGAGTTTGAGCAGCACAA+TGG | - | chr6.4:60953493-60953512 | None:intergenic | 40.0% | |
| TCACGAAGATGGTCTCATTA+AGG | + | chr6.4:60952998-60953017 | MS.gene059605:CDS | 40.0% | |
| TCCAATCATGGTGTGACTAT+AGG | + | chr6.4:60953584-60953603 | MS.gene059605:CDS | 40.0% | |
| TCCTATAGTCACACCATGAT+TGG | - | chr6.4:60953588-60953607 | None:intergenic | 40.0% | |
| AAAACGGATGTTGCATACCG+TGG | + | chr6.4:60953185-60953204 | MS.gene059605:CDS | 45.0% | |
| AACACGCAGTTTCAGTAGAG+AGG | + | chr6.4:60952907-60952926 | MS.gene059605:CDS | 45.0% | |
| ACAGTGTCACACTACGTAGA+TGG | + | chr6.4:60952939-60952958 | MS.gene059605:CDS | 45.0% | |
| AGACTAAGAACCATTGGGGA+GGG | + | chr6.4:60953156-60953175 | MS.gene059605:CDS | 45.0% | |
| CGAGAACATGGATTTGGAAG+TGG | + | chr6.4:60953456-60953475 | MS.gene059605:CDS | 45.0% | |
| GTGAAGAAATGGAACGGTCT+AGG | + | chr6.4:60953540-60953559 | MS.gene059605:CDS | 45.0% | |
| GTGCAGCAGAAGAGAAAGAA+GGG | + | chr6.4:60953236-60953255 | MS.gene059605:CDS | 45.0% | |
| TTGGGGAGGGATTACGAAAA+CGG | + | chr6.4:60953169-60953188 | MS.gene059605:CDS | 45.0% | |
| ! | AAAGCCTTGTGCAATCAGAG+TGG | + | chr6.4:60953265-60953284 | MS.gene059605:CDS | 45.0% |
| ! | TTCGTTTTCTACGGACCTAC+TGG | + | chr6.4:60953029-60953048 | MS.gene059605:CDS | 45.0% |
| ! | TTTTCGTAATCCCTCCCCAA+TGG | - | chr6.4:60953169-60953188 | None:intergenic | 45.0% |
| CGCAGTTTCAGTAGAGAGGA+AGG | + | chr6.4:60952911-60952930 | MS.gene059605:CDS | 50.0% | |
| GAGACTAAGAACCATTGGGG+AGG | + | chr6.4:60953155-60953174 | MS.gene059605:CDS | 50.0% | |
| GGTGCAGCAGAAGAGAAAGA+AGG | + | chr6.4:60953235-60953254 | MS.gene059605:CDS | 50.0% | |
| ! | TGTGCCACTCTGATTGCACA+AGG | - | chr6.4:60953272-60953291 | None:intergenic | 50.0% |
| ! | TTTCCATCGGAGAAGAGCGT+GGG | + | chr6.4:60952861-60952880 | MS.gene059605:CDS | 50.0% |
| ! | TTTTCCATCGGAGAAGAGCG+TGG | + | chr6.4:60952860-60952879 | MS.gene059605:CDS | 50.0% |
| GAACGGTCTAGGCAAAGCAG+AGG | + | chr6.4:60953551-60953570 | MS.gene059605:CDS | 55.0% | |
| GTTCCCACGCTCTTCTCCGA+TGG | - | chr6.4:60952867-60952886 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 60952849 | 60953673 | 60952849 | ID=MS.gene059605 |
| chr6.4 | mRNA | 60952849 | 60953673 | 60952849 | ID=MS.gene059605.t1;Parent=MS.gene059605 |
| chr6.4 | exon | 60952849 | 60953673 | 60952849 | ID=MS.gene059605.t1.exon1;Parent=MS.gene059605.t1 |
| chr6.4 | CDS | 60952849 | 60953673 | 60952849 | ID=cds.MS.gene059605.t1;Parent=MS.gene059605.t1 |
| Gene Sequence |
| Protein sequence |
