AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50875


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50875.t1 MTR_3g023760 80.652 491 49 10 1 468 1 468 0.0 684
MS.gene50875.t1 MTR_4g109340 40.741 432 188 20 1 402 1 394 8.46e-69 228
MS.gene50875.t1 MTR_4g109340 37.705 122 46 3 343 464 425 516 5.90e-16 80.5
MS.gene50875.t1 MTR_5g043930 33.065 248 119 7 7 220 1 235 7.15e-24 104
MS.gene50875.t1 MTR_4g082980 38.583 127 69 2 1 120 1 125 1.63e-15 79.0
MS.gene50875.t1 MTR_4g028610 24.033 491 246 20 5 463 8 403 5.23e-15 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50875.t1 AT1G26610 40.611 229 106 11 1 220 1 208 3.39e-30 122
MS.gene50875.t1 AT1G26590 28.444 225 109 7 1 220 1 178 1.45e-14 75.5
MS.gene50875.t1 AT1G26590 28.444 225 109 7 1 220 1 178 1.45e-14 75.5
MS.gene50875.t1 AT1G26590 28.444 225 109 7 1 220 1 178 1.45e-14 75.5
MS.gene50875.t1 AT1G26590 28.444 225 109 7 1 220 19 196 2.14e-14 75.1

Find 67 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCTTCTGTTGTGAATTT+TGG 0.147535 3.1:-14251143 MS.gene50875:CDS
AAACCAAAGAAGTTGGATTT+TGG 0.184575 3.1:-14250867 MS.gene50875:CDS
CCTTGTTCTTAACTTTCTTT+TGG 0.193235 3.1:+14251184 None:intergenic
TTAGAAACTAAATGAGTTAA+TGG 0.244636 3.1:+14250945 None:intergenic
TTGGATCTTCTCTTTCTCTT+TGG 0.274333 3.1:+14251203 None:intergenic
TCACCAAAATCCAACTTCTT+TGG 0.280627 3.1:+14250864 None:intergenic
AATGATCAAAGCAGTGAAAA+TGG 0.281007 3.1:-14250477 MS.gene50875:CDS
TTTCCTTCTGGTAGATCATT+AGG 0.296213 3.1:-14251578 MS.gene50875:CDS
TAATAATGATGATGATTCTT+TGG 0.335313 3.1:-14250979 MS.gene50875:CDS
AGAGTTTCTGTTATTGAGAA+TGG 0.358170 3.1:-14250786 MS.gene50875:CDS
TGATGATGATTCTTTGGAAT+TGG 0.360523 3.1:-14250973 MS.gene50875:CDS
AATGGATGAGAAGAAGAAAA+TGG 0.363640 3.1:-14250763 MS.gene50875:CDS
CATTTAGTTTCTAAGATTGA+TGG 0.365876 3.1:-14250936 MS.gene50875:CDS
TTTCAATCTTATCAAGCTCT+TGG 0.373280 3.1:-14250549 MS.gene50875:CDS
CAATCTGTGAAAGCTTTGTT+TGG 0.387025 3.1:-14251311 MS.gene50875:CDS
AAATGGAAACAGTACTGAAT+TGG 0.402383 3.1:-14250460 MS.gene50875:CDS
ACAAAATCAGGTACTACTAA+TGG 0.417832 3.1:-14250696 MS.gene50875:CDS
TGTGTAAAGAATGTGGTAAA+GGG 0.417853 3.1:-14251337 MS.gene50875:CDS
ACTTCCTTCTTCTTCCAACT+TGG 0.433264 3.1:+14250833 None:intergenic
ATGAAGAAGAAAGAAGGTTA+TGG 0.434835 3.1:-14251455 MS.gene50875:CDS
TTCTGATAATAATGATGAAA+AGG 0.437329 3.1:-14251090 MS.gene50875:CDS
TTACATCAGAAGGTGAATTC+AGG 0.437398 3.1:-14250626 MS.gene50875:CDS
TGATTCTGATTTAGCATTTG+AGG 0.447267 3.1:-14250730 MS.gene50875:CDS
TTGTGTAAAGAATGTGGTAA+AGG 0.465384 3.1:-14251338 MS.gene50875:CDS
GTCAATGTAAGAACAAAATC+AGG 0.466460 3.1:-14250708 MS.gene50875:CDS
TTATCCAAAACAACATCAAA+AGG 0.478081 3.1:+14251362 None:intergenic
TACAAACCATGGTGGTTAGT+TGG 0.485401 3.1:-14250294 MS.gene50875:CDS
CAATCTTATCAAGCTCTTGG+AGG 0.491208 3.1:-14250546 MS.gene50875:CDS
GTGAAAGAGTTGCTGAAATG+AGG 0.492259 3.1:-14250389 MS.gene50875:CDS
GTCATAGAGCTAGTCATAAG+AGG 0.492989 3.1:-14250524 MS.gene50875:CDS
AGAAGAATGAAGAAGAAAGA+AGG 0.493856 3.1:-14251461 MS.gene50875:CDS
TACTAATGGTACTTCAATGA+AGG 0.501936 3.1:-14250682 MS.gene50875:CDS
GAAGAAGAAAATGGAGATTG+AGG 0.502348 3.1:-14250754 MS.gene50875:CDS
TTGGAAGAAGAAGGAAGTGA+TGG 0.508641 3.1:-14250828 MS.gene50875:CDS
TCATCATCAATACTTGCAGC+AGG 0.511776 3.1:+14250339 None:intergenic
ATTTAGTTTCTAAGATTGAT+GGG 0.512601 3.1:-14250935 MS.gene50875:CDS
TGAAAGAGTTGCTGAAATGA+GGG 0.515667 3.1:-14250388 MS.gene50875:CDS
AAGTCCAAGTTGGAAGAAGA+AGG 0.518126 3.1:-14250837 MS.gene50875:CDS
AGTATTGATGATGAAAGAAA+TGG 0.523984 3.1:-14250330 MS.gene50875:CDS
GCTAGTCATAAGAGGACCAA+GGG 0.524564 3.1:-14250516 MS.gene50875:CDS
AGAGATGTAAGACCTTGGTT+TGG 0.525051 3.1:-14251029 MS.gene50875:CDS
AAGAGAAAATATCAGAAAAG+AGG 0.527607 3.1:-14250603 MS.gene50875:CDS
TTCTGTTATTGAGAATGGAA+TGG 0.538325 3.1:-14250781 MS.gene50875:CDS
TGTAAATGCTGAGGGAAGAA+TGG 0.552087 3.1:-14250418 MS.gene50875:CDS
TACTGAATTGGAAGTAGATG+TGG 0.557897 3.1:-14250448 MS.gene50875:CDS
ATCAAGCAAGAGGCAATGAT+AGG 0.560531 3.1:-14250264 MS.gene50875:CDS
CCTTCTGGTAGATCATTAGG+TGG 0.567747 3.1:-14251575 MS.gene50875:CDS
GATAAATTGTGTAAAGAATG+TGG 0.571592 3.1:-14251344 MS.gene50875:CDS
TGATGAAAAGGAACAAGAAG+AGG 0.575044 3.1:-14251078 MS.gene50875:CDS
TCTTGAAGCAGAAAACCCCT+TGG 0.580349 3.1:+14250500 None:intergenic
AAGGTTACACTTACATCAGA+AGG 0.589040 3.1:-14250636 MS.gene50875:CDS
GATTATGATCATGATGATGA+TGG 0.591943 3.1:-14251509 MS.gene50875:CDS
TTAGTAGAGATGTAAGACCT+TGG 0.592699 3.1:-14251034 MS.gene50875:CDS
GAGAGAATCCAAAGAAAACA+TGG 0.594959 3.1:-14251427 MS.gene50875:CDS
TGGTGATGTTAAGTCCAAGT+TGG 0.597669 3.1:-14250847 MS.gene50875:CDS
TGGAAGCAGTATCAAGCAAG+AGG 0.613537 3.1:-14250274 MS.gene50875:CDS
GATCATTAGGTGGTCACATG+AGG 0.626071 3.1:-14251565 MS.gene50875:CDS
ATGCTGAGATTTACAAACCA+TGG 0.628225 3.1:-14250305 MS.gene50875:CDS
CCAAAAGAAAGTTAAGAACA+AGG 0.628452 3.1:-14251184 MS.gene50875:CDS
AGCTAGTCATAAGAGGACCA+AGG 0.642709 3.1:-14250517 MS.gene50875:CDS
CCACCTAATGATCTACCAGA+AGG 0.655089 3.1:+14251575 None:intergenic
GGAGATGAATGTAAATGCTG+AGG 0.667074 3.1:-14250427 MS.gene50875:CDS
GAGATGAATGTAAATGCTGA+GGG 0.668731 3.1:-14250426 MS.gene50875:CDS
CTGCTTCCAACTAACCACCA+TGG 0.679286 3.1:+14250288 None:intergenic
CTGAGATTTACAAACCATGG+TGG 0.688746 3.1:-14250302 MS.gene50875:CDS
CTAGTCATAAGAGGACCAAG+GGG 0.693462 3.1:-14250515 MS.gene50875:CDS
ATAGAATTGATACCAAACCA+AGG 0.704170 3.1:+14251017 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAGAAACTAAATGAGTTAA+TGG + chr3.1:14250938-14250957 None:intergenic 20.0%
!! TTCTGATAATAATGATGAAA+AGG - chr3.1:14250790-14250809 MS.gene50875:CDS 20.0%
!!! ATTTAGTTTCTAAGATTGAT+GGG - chr3.1:14250945-14250964 MS.gene50875:CDS 20.0%
!!! TAATAATGATGATGATTCTT+TGG - chr3.1:14250901-14250920 MS.gene50875:CDS 20.0%
! AAGAGAAAATATCAGAAAAG+AGG - chr3.1:14251277-14251296 MS.gene50875:CDS 25.0%
! GATAAATTGTGTAAAGAATG+TGG - chr3.1:14250536-14250555 MS.gene50875:CDS 25.0%
! TTATCCAAAACAACATCAAA+AGG + chr3.1:14250521-14250540 None:intergenic 25.0%
!!! AGTATTGATGATGAAAGAAA+TGG - chr3.1:14251550-14251569 MS.gene50875:CDS 25.0%
!!! CATTTAGTTTCTAAGATTGA+TGG - chr3.1:14250944-14250963 MS.gene50875:CDS 25.0%
!!! GAAGAATAAGAGTTTTGAAT+TGG - chr3.1:14250610-14250629 MS.gene50875:CDS 25.0%
!!! TTTTGTTGATTCTGAAACTA+AGG - chr3.1:14251225-14251244 MS.gene50875:CDS 25.0%
AATGATCAAAGCAGTGAAAA+TGG - chr3.1:14251403-14251422 MS.gene50875:CDS 30.0%
AATGGATGAGAAGAAGAAAA+TGG - chr3.1:14251117-14251136 MS.gene50875:CDS 30.0%
ACAAAATCAGGTACTACTAA+TGG - chr3.1:14251184-14251203 MS.gene50875:CDS 30.0%
AGAAGAATGAAGAAGAAAGA+AGG - chr3.1:14250419-14250438 MS.gene50875:CDS 30.0%
AGAGTTTCTGTTATTGAGAA+TGG - chr3.1:14251094-14251113 MS.gene50875:CDS 30.0%
ATAGAATTGATACCAAACCA+AGG + chr3.1:14250866-14250885 None:intergenic 30.0%
ATGAAGAAGAAAGAAGGTTA+TGG - chr3.1:14250425-14250444 MS.gene50875:CDS 30.0%
CCAAAAGAAAGTTAAGAACA+AGG - chr3.1:14250696-14250715 MS.gene50875:CDS 30.0%
GAACAAAAAACCAAAGAAGT+TGG - chr3.1:14251006-14251025 MS.gene50875:CDS 30.0%
GTCAATGTAAGAACAAAATC+AGG - chr3.1:14251172-14251191 MS.gene50875:CDS 30.0%
TACTAATGGTACTTCAATGA+AGG - chr3.1:14251198-14251217 MS.gene50875:CDS 30.0%
TGATTCTGATTTAGCATTTG+AGG - chr3.1:14251150-14251169 MS.gene50875:CDS 30.0%
TGTGTAAAGAATGTGGTAAA+GGG - chr3.1:14250543-14250562 MS.gene50875:CDS 30.0%
TTCTGTTATTGAGAATGGAA+TGG - chr3.1:14251099-14251118 MS.gene50875:CDS 30.0%
TTGTGTAAAGAATGTGGTAA+AGG - chr3.1:14250542-14250561 MS.gene50875:CDS 30.0%
TTTCAATCTTATCAAGCTCT+TGG - chr3.1:14251331-14251350 MS.gene50875:CDS 30.0%
! AAACCAAAGAAGTTGGATTT+TGG - chr3.1:14251013-14251032 MS.gene50875:CDS 30.0%
! AAATGGAAACAGTACTGAAT+TGG - chr3.1:14251420-14251439 MS.gene50875:CDS 30.0%
! ACTTCTTCTGTTGTGAATTT+TGG - chr3.1:14250737-14250756 MS.gene50875:CDS 30.0%
! CCTTGTTCTTAACTTTCTTT+TGG + chr3.1:14250699-14250718 None:intergenic 30.0%
! GATTATGATCATGATGATGA+TGG - chr3.1:14250371-14250390 MS.gene50875:CDS 30.0%
!! AGATCCTTTTGATGTTGTTT+TGG - chr3.1:14250514-14250533 MS.gene50875:CDS 30.0%
!! TGATGATGATTCTTTGGAAT+TGG - chr3.1:14250907-14250926 MS.gene50875:CDS 30.0%
AAGGTTACACTTACATCAGA+AGG - chr3.1:14251244-14251263 MS.gene50875:CDS 35.0%
ATGCTGAGATTTACAAACCA+TGG - chr3.1:14251575-14251594 MS.gene50875:CDS 35.0%
GAAGAAGAAAATGGAGATTG+AGG - chr3.1:14251126-14251145 MS.gene50875:CDS 35.0%
GAGAGAATCCAAAGAAAACA+TGG - chr3.1:14250453-14250472 MS.gene50875:CDS 35.0%
GAGATGAATGTAAATGCTGA+GGG - chr3.1:14251454-14251473 MS.gene50875:CDS 35.0%
TACTGAATTGGAAGTAGATG+TGG - chr3.1:14251432-14251451 MS.gene50875:CDS 35.0%
TCACCAAAATCCAACTTCTT+TGG + chr3.1:14251019-14251038 None:intergenic 35.0%
TGAAAGAGTTGCTGAAATGA+GGG - chr3.1:14251492-14251511 MS.gene50875:CDS 35.0%
TGATGAAAAGGAACAAGAAG+AGG - chr3.1:14250802-14250821 MS.gene50875:CDS 35.0%
TTACATCAGAAGGTGAATTC+AGG - chr3.1:14251254-14251273 MS.gene50875:CDS 35.0%
TTAGTAGAGATGTAAGACCT+TGG - chr3.1:14250846-14250865 MS.gene50875:CDS 35.0%
TTGGATCTTCTCTTTCTCTT+TGG + chr3.1:14250680-14250699 None:intergenic 35.0%
TTTCCTTCTGGTAGATCATT+AGG - chr3.1:14250302-14250321 MS.gene50875:CDS 35.0%
! AGTACTCTCCATGTTTTCTT+TGG + chr3.1:14250464-14250483 None:intergenic 35.0%
! TGCAGAAAGAATTTTCCTTC+TGG - chr3.1:14250290-14250309 MS.gene50875:CDS 35.0%
!! CAATCTGTGAAAGCTTTGTT+TGG - chr3.1:14250569-14250588 MS.gene50875:CDS 35.0%
ACTTCCTTCTTCTTCCAACT+TGG + chr3.1:14251050-14251069 None:intergenic 40.0%
AGAGATGTAAGACCTTGGTT+TGG - chr3.1:14250851-14250870 MS.gene50875:CDS 40.0%
ATCAAGCAAGAGGCAATGAT+AGG - chr3.1:14251616-14251635 MS.gene50875:CDS 40.0%
CAATCTTATCAAGCTCTTGG+AGG - chr3.1:14251334-14251353 MS.gene50875:CDS 40.0%
CTGAGATTTACAAACCATGG+TGG - chr3.1:14251578-14251597 MS.gene50875:CDS 40.0%
GGAGATGAATGTAAATGCTG+AGG - chr3.1:14251453-14251472 MS.gene50875:CDS 40.0%
GTCATAGAGCTAGTCATAAG+AGG - chr3.1:14251356-14251375 MS.gene50875:CDS 40.0%
GTGAAAGAGTTGCTGAAATG+AGG - chr3.1:14251491-14251510 MS.gene50875:CDS 40.0%
TACAAACCATGGTGGTTAGT+TGG - chr3.1:14251586-14251605 MS.gene50875:CDS 40.0%
TCATCATCAATACTTGCAGC+AGG + chr3.1:14251544-14251563 None:intergenic 40.0%
TGTAAATGCTGAGGGAAGAA+TGG - chr3.1:14251462-14251481 MS.gene50875:CDS 40.0%
TTGGAAGAAGAAGGAAGTGA+TGG - chr3.1:14251052-14251071 MS.gene50875:CDS 40.0%
! AAGTCCAAGTTGGAAGAAGA+AGG - chr3.1:14251043-14251062 MS.gene50875:CDS 40.0%
! TGGTGATGTTAAGTCCAAGT+TGG - chr3.1:14251033-14251052 MS.gene50875:CDS 40.0%
AGCTAGTCATAAGAGGACCA+AGG - chr3.1:14251363-14251382 MS.gene50875:CDS 45.0%
CCACCTAATGATCTACCAGA+AGG + chr3.1:14250308-14250327 None:intergenic 45.0%
CCTTCTGGTAGATCATTAGG+TGG - chr3.1:14250305-14250324 MS.gene50875:CDS 45.0%
CTAGTCATAAGAGGACCAAG+GGG - chr3.1:14251365-14251384 MS.gene50875:CDS 45.0%
GATCATTAGGTGGTCACATG+AGG - chr3.1:14250315-14250334 MS.gene50875:CDS 45.0%
GCTAGTCATAAGAGGACCAA+GGG - chr3.1:14251364-14251383 MS.gene50875:CDS 45.0%
TCTTGAAGCAGAAAACCCCT+TGG + chr3.1:14251383-14251402 None:intergenic 45.0%
TGGAAGCAGTATCAAGCAAG+AGG - chr3.1:14251606-14251625 MS.gene50875:CDS 45.0%
CTGCTTCCAACTAACCACCA+TGG + chr3.1:14251595-14251614 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 14250248 14251654 14250248 ID=MS.gene50875
chr3.1 mRNA 14250248 14251654 14250248 ID=MS.gene50875.t1;Parent=MS.gene50875
chr3.1 exon 14250248 14251654 14250248 ID=MS.gene50875.t1.exon1;Parent=MS.gene50875.t1
chr3.1 CDS 14250248 14251654 14250248 ID=cds.MS.gene50875.t1;Parent=MS.gene50875.t1
Gene Sequence

>MS.gene50875

ATGGAAGAAGATCAAGAAATGATGAAACATGTGTGCAAATTCTGCAGAAAGAATTTTCCTTCTGGTAGATCATTAGGTGGTCACATGAGGTCTCATGATCATGATCATGATCATGATCATGATGATTATGATCATGATGATGATGGTGATGAGTTTGTTGTTGCTGTAAGAAGAAGAATGAAGAAGAAAGAAGGTTATGGTCTTAGAGAGAATCCAAAGAAAACATGGAGAGTACTCACTGATTCAACTAATGAACAAGAGATTCAAGATCCTTTTGATGTTGTTTTGGATAAATTGTGTAAAGAATGTGGTAAAGGGTTTCAATCTGTGAAAGCTTTGTTTGGTCACATGAAGTGTCATAAGAAGAATAAGAGTTTTGAATTGGATGAAGATGAAGAAGAAGAAGAAGATTCTAATGCAACTGTTCTTCCAAAGAGAAAGAGAAGATCCAAAAGAAAGTTAAGAACAAGGTACAATAAGAGTAATAACACTTCTTCTGTTGTGAATTTTGGTGATAATTCTTTAGATTCTATTGCTGAATCTTCTGATAATAATGATGAAAAGGAACAAGAAGAGGTTGCTATGAGTTTGATCATGCTTAGTAGAGATGTAAGACCTTGGTTTGGTATCAATTCTATTGCTGAATTTTCTGATAATAATGATGATGATTCTTTGGAATTGGAAGCGCCATTAACTCATTTAGTTTCTAAGATTGATGGGAAAAAGTTTATTACTAATAGTTCTGAAATTGTGAAAATGAACAAAAAACCAAAGAAGTTGGATTTTGGTGATGTTAAGTCCAAGTTGGAAGAAGAAGGAAGTGATGGATTGAAGATGAAGAAAGCTAGAGTTTCTGTTATTGAGAATGGAATGGATGAGAAGAAGAAAATGGAGATTGAGGTTGATTCTGATTTAGCATTTGAGGTCAATGTAAGAACAAAATCAGGTACTACTAATGGTACTTCAATGAAGGAAAATTTTGTTGATTCTGAAACTAAGGTTACACTTACATCAGAAGGTGAATTCAGGAAGAGAAAATATCAGAAAAGAGGAAAATTTGAATGTGCTACTTGCAACAAAACATTTCAATCTTATCAAGCTCTTGGAGGTCATAGAGCTAGTCATAAGAGGACCAAGGGGTTTTCTGCTTCAAGAAATGATCAAAGCAGTGAAAATGGAAACAGTACTGAATTGGAAGTAGATGTGGAGATGAATGTAAATGCTGAGGGAAGAATGGTAGAAAGTGAAAGAGTTGCTGAAATGAGGGATTATCTTGATCTTGATCTTAATCTTCCTGCTGCAAGTATTGATGATGAAAGAAATGGTCATGCTGAGATTTACAAACCATGGTGGTTAGTTGGAAGCAGTATCAAGCAAGAGGCAATGATAGGTCTACTATCAAACTAG

Protein sequence

>MS.gene50875.t1

MEEDQEMMKHVCKFCRKNFPSGRSLGGHMRSHDHDHDHDHDDYDHDDDGDEFVVAVRRRMKKKEGYGLRENPKKTWRVLTDSTNEQEIQDPFDVVLDKLCKECGKGFQSVKALFGHMKCHKKNKSFELDEDEEEEEDSNATVLPKRKRRSKRKLRTRYNKSNNTSSVVNFGDNSLDSIAESSDNNDEKEQEEVAMSLIMLSRDVRPWFGINSIAEFSDNNDDDSLELEAPLTHLVSKIDGKKFITNSSEIVKMNKKPKKLDFGDVKSKLEEEGSDGLKMKKARVSVIENGMDEKKKMEIEVDSDLAFEVNVRTKSGTTNGTSMKENFVDSETKVTLTSEGEFRKRKYQKRGKFECATCNKTFQSYQALGGHRASHKRTKGFSASRNDQSSENGNSTELEVDVEMNVNAEGRMVESERVAEMRDYLDLDLNLPAASIDDERNGHAEIYKPWWLVGSSIKQEAMIGLLSN