AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061735

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061735.t1 MTR_4g108880 97.778 585 13 0 1 585 1 585 0.0 1202
MS.gene061735.t1 MTR_3g037390 63.484 597 157 7 19 585 10 575 0.0 729
MS.gene061735.t1 MTR_3g037390 60.359 502 138 7 114 585 1 471 0.0 573
MS.gene061735.t1 MTR_4g061360 44.531 128 71 0 27 154 83 210 1.37e-31 130
MS.gene061735.t1 MTR_4g061360 70.833 48 14 0 501 548 717 764 4.55e-16 82.4
MS.gene061735.t1 MTR_3g092780 44.186 129 72 0 27 155 50 178 6.71e-30 125
MS.gene061735.t1 MTR_3g092780 73.333 45 12 0 505 549 589 633 5.95e-14 75.5
MS.gene061735.t1 MTR_3g092780 44.186 129 72 0 27 155 50 178 6.80e-30 125
MS.gene061735.t1 MTR_3g092780 73.333 45 12 0 505 549 590 634 6.01e-14 75.5
MS.gene061735.t1 MTR_3g092780 44.186 129 72 0 27 155 50 178 7.44e-30 125
MS.gene061735.t1 MTR_3g092780 73.333 45 12 0 505 549 631 675 5.79e-14 75.5
MS.gene061735.t1 MTR_3g092780 44.186 129 72 0 27 155 50 178 8.05e-30 125
MS.gene061735.t1 MTR_3g092780 73.333 45 12 0 505 549 630 674 5.79e-14 75.5
MS.gene061735.t1 MTR_3g037400 59.633 109 37 4 46 151 144 248 9.93e-30 121
MS.gene061735.t1 MTR_7g118260 33.816 207 123 5 27 229 8 204 8.89e-28 118
MS.gene061735.t1 MTR_7g118260 64.000 50 18 0 501 550 537 586 1.71e-14 77.0
MS.gene061735.t1 MTR_7g118260 40.580 138 78 1 17 154 2 135 1.43e-27 117
MS.gene061735.t1 MTR_7g118260 64.000 50 18 0 501 550 500 549 2.18e-14 76.6
MS.gene061735.t1 MTR_8g024260 39.844 128 77 0 27 154 30 157 4.76e-27 116
MS.gene061735.t1 MTR_8g024260 71.111 45 13 0 504 548 583 627 5.60e-13 72.4
MS.gene061735.t1 MTR_1g067110 40.625 128 76 0 27 154 79 206 6.45e-26 113
MS.gene061735.t1 MTR_1g067110 68.085 47 12 1 504 547 690 736 1.28e-12 71.2
MS.gene061735.t1 MTR_1g067110 40.625 128 76 0 27 154 79 206 6.49e-26 113
MS.gene061735.t1 MTR_3g086100 36.585 123 75 1 33 155 17 136 1.69e-20 95.9
MS.gene061735.t1 MTR_2g450070 34.286 140 87 2 19 156 6 142 1.03e-19 93.6
MS.gene061735.t1 MTR_2g450070 34.286 140 87 2 19 156 6 142 1.09e-19 93.6
MS.gene061735.t1 MTR_2g034960 36.232 138 83 2 21 156 19 153 1.14e-19 93.2
MS.gene061735.t1 MTR_4g121020 35.766 137 82 3 22 156 23 155 1.81e-19 92.8
MS.gene061735.t1 MTR_4g121020 35.766 137 82 3 22 156 23 155 1.83e-19 92.8
MS.gene061735.t1 MTR_1g032570 34.426 122 77 1 33 154 15 133 1.91e-19 92.8
MS.gene061735.t1 MTR_3g102600 36.842 133 79 2 25 155 2 131 6.63e-19 85.5
MS.gene061735.t1 MTR_3g106220 34.328 134 83 2 23 156 4 132 1.24e-18 90.1
MS.gene061735.t1 MTR_4g131580 34.146 123 78 1 33 155 21 140 1.43e-18 89.7
MS.gene061735.t1 MTR_3g102600 36.842 133 79 2 25 155 2 131 3.92e-18 84.3
MS.gene061735.t1 MTR_8g079940 34.959 123 77 1 33 155 29 148 6.02e-18 87.8
MS.gene061735.t1 MTR_1g013170 32.258 124 81 1 33 156 9 129 1.84e-17 85.9
MS.gene061735.t1 MTR_4g131600 31.061 132 86 2 24 155 17 143 4.68e-17 85.1
MS.gene061735.t1 MTR_7g117705 31.159 138 91 2 33 169 9 143 1.68e-16 82.8
MS.gene061735.t1 MTR_1g013180 32.258 124 81 1 33 156 160 280 3.15e-16 82.8
MS.gene061735.t1 MTR_4g131570 27.174 184 104 3 33 211 21 179 1.70e-15 80.1
MS.gene061735.t1 MTR_7g118260 64.000 50 18 0 501 550 367 416 7.49e-15 77.4
MS.gene061735.t1 MTR_7g118260 62.745 51 19 0 500 550 450 500 8.11e-15 77.8
MS.gene061735.t1 MTR_3g102600 39.474 76 43 1 80 155 10 82 1.36e-13 68.6
MS.gene061735.t1 MTR_3g102590 32.258 124 82 1 33 156 12 133 6.26e-13 69.3
MS.gene061735.t1 MTR_3g102600 39.474 76 43 1 80 155 10 82 7.27e-13 67.8
MS.gene061735.t1 MTR_1g067110 68.085 47 12 1 504 547 525 571 9.35e-13 71.2
MS.gene061735.t1 MTR_3g102590 32.258 124 82 1 33 156 12 133 1.04e-12 67.8
MS.gene061735.t1 MTR_3g037450 72.093 43 8 1 114 156 1 39 3.32e-12 62.4
MS.gene061735.t1 MTR_5g014040 29.134 127 84 3 26 151 10 131 1.41e-11 67.4
MS.gene061735.t1 MTR_5g014040 29.134 127 84 3 26 151 10 131 1.58e-11 67.4
MS.gene061735.t1 MTR_4g093730 64.444 45 16 0 501 545 104 148 2.24e-11 65.9
MS.gene061735.t1 MTR_5g066510 52.459 61 27 1 505 563 135 195 2.66e-11 63.5
MS.gene061735.t1 MTR_0450s0040 27.869 122 85 1 29 150 4 122 7.65e-11 64.3
MS.gene061735.t1 MTR_7g490310 25.676 148 92 1 26 155 2 149 8.18e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061735.t1 AT5G61380 50.000 636 215 18 22 584 8 613 0.0 524
MS.gene061735.t1 AT5G60100 45.902 122 66 0 33 154 64 185 1.02e-30 124
MS.gene061735.t1 AT5G60100 45.902 122 66 0 33 154 64 185 1.02e-30 124
MS.gene061735.t1 AT5G60100 45.902 122 66 0 33 154 64 185 1.02e-30 124
MS.gene061735.t1 AT5G60100 45.902 122 66 0 33 154 64 185 2.01e-30 125
MS.gene061735.t1 AT5G60100 60.606 66 19 2 489 547 419 484 3.48e-15 79.0
MS.gene061735.t1 AT5G60100 45.902 122 66 0 33 154 64 185 2.01e-30 125
MS.gene061735.t1 AT5G60100 60.606 66 19 2 489 547 419 484 3.48e-15 79.0
MS.gene061735.t1 AT5G60100 45.902 122 66 0 33 154 64 185 2.01e-30 125
MS.gene061735.t1 AT5G60100 60.606 66 19 2 489 547 419 484 3.48e-15 79.0
MS.gene061735.t1 AT5G24470 43.609 133 75 0 27 159 44 176 4.06e-30 125
MS.gene061735.t1 AT5G24470 66.667 45 15 0 504 548 508 552 2.22e-13 73.6
MS.gene061735.t1 AT5G60100 45.902 122 66 0 33 154 64 185 7.58e-30 124
MS.gene061735.t1 AT5G60100 60.606 66 19 2 489 547 446 511 5.37e-15 78.6
MS.gene061735.t1 AT2G46790 40.625 128 76 0 27 154 31 158 1.39e-29 122
MS.gene061735.t1 AT2G46790 40.625 128 76 0 27 154 31 158 1.64e-29 122
MS.gene061735.t1 AT2G46790 76.087 46 11 0 504 549 416 461 2.96e-16 82.0
MS.gene061735.t1 AT5G02810 42.969 128 73 0 27 154 72 199 6.11e-27 116
MS.gene061735.t1 AT5G02810 74.510 51 13 0 497 547 661 711 6.32e-17 85.1
MS.gene061735.t1 AT3G16857 36.508 126 77 1 31 156 35 157 1.43e-20 96.3
MS.gene061735.t1 AT3G16857 36.508 126 77 1 31 156 35 157 1.58e-20 96.3
MS.gene061735.t1 AT2G25180 34.959 123 77 1 33 155 17 136 8.82e-20 93.6
MS.gene061735.t1 AT4G31920 35.537 121 75 1 33 153 17 134 3.03e-19 91.7
MS.gene061735.t1 AT1G67710 35.772 123 76 1 33 155 11 130 1.34e-18 89.7
MS.gene061735.t1 AT2G46670 77.778 45 10 0 505 549 132 176 1.08e-17 81.6
MS.gene061735.t1 AT5G02810 74.510 51 13 0 497 547 517 567 4.74e-17 85.1
MS.gene061735.t1 AT2G46790 76.087 46 11 0 504 549 259 304 6.13e-17 82.4
MS.gene061735.t1 AT2G46790 76.087 46 11 0 504 549 259 304 6.13e-17 82.4
MS.gene061735.t1 AT2G46790 76.087 46 11 0 504 549 299 344 1.60e-16 81.6
MS.gene061735.t1 AT2G46790 63.415 41 15 0 114 154 1 41 6.58e-13 70.9
MS.gene061735.t1 AT2G01760 32.800 125 82 1 31 155 9 131 7.56e-15 77.0
MS.gene061735.t1 AT3G16857 42.308 78 42 1 79 156 13 87 1.36e-14 77.4
MS.gene061735.t1 AT5G58080 33.333 132 82 3 25 154 8 135 8.66e-14 74.7
MS.gene061735.t1 AT3G62670 30.233 129 84 3 27 155 36 158 3.72e-13 72.0
MS.gene061735.t1 AT2G01760 31.532 111 74 1 45 155 51 159 1.80e-11 66.6

Find 145 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATACTCATGGGAAAATA+TGG 0.142897 8.1:+16509927 None:intergenic
TCTTATTAATTGCTCTTATC+AGG 0.143425 8.1:-16513302 MS.gene061735:CDS
GAACCATAACTTCCATTTCT+TGG 0.183017 8.1:+16510155 None:intergenic
CTCGGGATTATTGCTTCTTT+TGG 0.197660 8.1:+16511299 None:intergenic
GTTGCTTCGATAAGAAAATT+AGG 0.301363 8.1:-16509770 MS.gene061735:CDS
GGTATTAATGTGGATCTAAA+TGG 0.303023 8.1:-16509678 MS.gene061735:CDS
CAGAAAGGCGGCCTCGTGTT+AGG 0.304907 8.1:-16509725 MS.gene061735:CDS
TGCTTTGACATAAGTGAAAA+AGG 0.328177 8.1:+16510466 None:intergenic
GACCTTATAAGACATGAAAA+TGG 0.329865 8.1:-16510335 MS.gene061735:CDS
GTTACAAATAGTAGCTTTGA+TGG 0.334131 8.1:-16510708 MS.gene061735:intron
AAATTTCCGCCTGTTCTTGT+TGG 0.352269 8.1:+16511262 None:intergenic
CAATGTCCACCTCATGCAAC+TGG 0.364014 8.1:-16509963 MS.gene061735:CDS
TAACATTCACCTCTTCTTGT+TGG 0.368007 8.1:+16511235 None:intergenic
CTTCTTCGATCACTAATTCC+AGG 0.369829 8.1:+16511000 None:intergenic
CACTGCTGCTGCTGTTATTG+AGG 0.387890 8.1:-16511064 MS.gene061735:CDS
TAACATGACTCTGAAGGTTC+TGG 0.390005 8.1:+16510021 None:intergenic
CTAATGAACTATTAAATTTG+TGG 0.394489 8.1:-16511963 MS.gene061735:CDS
GCTCATCCATGGCCGTCATT+AGG 0.398082 8.1:-16509879 MS.gene061735:CDS
CATCCAAGAAATGGAAGTTA+TGG 0.399698 8.1:-16510158 MS.gene061735:CDS
AGTTGCATGAGGTGGACATT+GGG 0.404126 8.1:+16509965 None:intergenic
AAGAAGAGTGAACTCAGAAT+TGG 0.405640 8.1:-16510500 MS.gene061735:CDS
AACTAAAGCTTACTTATAAT+AGG 0.406920 8.1:+16512163 None:intergenic
TCGGGGATACTATTGCTTCT+AGG 0.411593 8.1:+16510278 None:intergenic
ATTCTGAGTTCACTCTTCTT+CGG 0.411743 8.1:+16510503 None:intergenic
CTGTTTGTATTGGCATCACT+AGG 0.416022 8.1:+16511357 None:intergenic
GTGTTAGGGGACAGTTTGTC+AGG 0.418641 8.1:-16509710 MS.gene061735:CDS
GCTTACTTATAATAGGAATT+CGG 0.421211 8.1:+16512170 None:intergenic
AGAAAGGCGGCCTCGTGTTA+GGG 0.423017 8.1:-16509724 MS.gene061735:CDS
ATTGGGGAAGTTGATTGTAC+TGG 0.429425 8.1:+16509982 None:intergenic
CTCTCAACAGAGAAAGAATC+GGG 0.433841 8.1:+16510260 None:intergenic
TCATTGATAGGAGTAAAGTT+AGG 0.434757 8.1:-16513375 MS.gene061735:CDS
GAAGGTGGCACTGTTTGTAT+TGG 0.449438 8.1:+16511347 None:intergenic
TGACCTTCTCAAGCATCCTT+AGG 0.451954 8.1:+16509582 None:intergenic
TAGGTAAGTCATCTTGTGAA+TGG 0.453633 8.1:+16510297 None:intergenic
GGGGTGGTTCATCCAAGAAA+TGG 0.454607 8.1:-16510167 MS.gene061735:CDS
GTGTACCCCACCTGCATCAA+TGG 0.457279 8.1:-16510234 MS.gene061735:CDS
TGGTTCTGAGCTCAACCAAT+CGG 0.464264 8.1:-16510138 MS.gene061735:CDS
AATAATAGCAAGAGTGGTGA+TGG 0.468780 8.1:-16513400 MS.gene061735:CDS
ATTAGTGATCGAAGAAGAAC+AGG 0.469944 8.1:-16510994 MS.gene061735:intron
TATTCTGATACATGAACATA+TGG 0.472895 8.1:+16511036 None:intergenic
AACAGTAAAGAGGTTAATTG+TGG 0.476504 8.1:-16513427 MS.gene061735:CDS
AATAGTTCATTAGTACGTAA+AGG 0.484282 8.1:+16511973 None:intergenic
GCAACTCCTGGCATATAATA+CGG 0.486587 8.1:+16510092 None:intergenic
AATTTCAATCCAACAAGAAC+AGG 0.489300 8.1:-16511271 MS.gene061735:CDS
TATTGATTTGAACAGTAAAG+AGG 0.489414 8.1:-16513437 MS.gene061735:CDS
CACAGCAAAATAGTAATCAC+AGG 0.492222 8.1:-16510205 MS.gene061735:CDS
TCTCTCAACAGAGAAAGAAT+CGG 0.495017 8.1:+16510259 None:intergenic
TCGTCATAGTCGGTGGAAGC+AGG 0.498204 8.1:+16509651 None:intergenic
TCACTAATTCCAGGTACATC+AGG 0.499237 8.1:+16511009 None:intergenic
AGAAGCAATAATCCCGAGGC+TGG 0.500098 8.1:-16511294 MS.gene061735:CDS
CTTATTAATTGCTCTTATCA+GGG 0.502067 8.1:-16513301 MS.gene061735:intron
AAATACTCATGGGAAAATAT+GGG 0.502365 8.1:+16509928 None:intergenic
ATAATACGGATAAGCATGTT+GGG 0.502826 8.1:+16510106 None:intergenic
AGAAAATTAGGTACGTCAAT+CGG 0.504195 8.1:-16509758 MS.gene061735:CDS
GTTGGGCAGGCATGCCCGAT+TGG 0.504294 8.1:+16510123 None:intergenic
TTGGATTGAAATTCCAGCCT+CGG 0.504832 8.1:+16511281 None:intergenic
TGGATTGAAATTCCAGCCTC+GGG 0.511665 8.1:+16511282 None:intergenic
GAATATCAGCCTGATGTACC+TGG 0.511952 8.1:-16511018 MS.gene061735:CDS
GTGGTGATGGGTTCATTGAT+AGG 0.512716 8.1:-16513387 MS.gene061735:CDS
CAGTTTGTCAGGAAGTTGAA+TGG 0.513514 8.1:-16509699 MS.gene061735:CDS
TACGGATAAGCATGTTGGGC+AGG 0.516130 8.1:+16510110 None:intergenic
TATAATACGGATAAGCATGT+TGG 0.516804 8.1:+16510105 None:intergenic
TATTTGTTTACAGACCGGTC+AGG 0.523053 8.1:-16509910 MS.gene061735:CDS
GGTTCTGAGCTCAACCAATC+GGG 0.529063 8.1:-16510137 MS.gene061735:CDS
GTTGTCAAGTGCTTGAGGCT+CGG 0.530125 8.1:-16512018 MS.gene061735:CDS
GCTTATCCGTATTATATGCC+AGG 0.531404 8.1:-16510098 MS.gene061735:CDS
GTCTGTGTCATCAGAGAAGA+AGG 0.532621 8.1:+16511329 None:intergenic
AAGACATGAAAATGGAGAAA+CGG 0.538385 8.1:-16510327 MS.gene061735:CDS
CACCAGGGATTCCTCTCCTA+AGG 0.541039 8.1:-16509598 MS.gene061735:CDS
CAGTTGCATGAGGTGGACAT+TGG 0.541645 8.1:+16509964 None:intergenic
ATGAGTATTTGTTTACAGAC+CGG 0.543051 8.1:-16509915 MS.gene061735:CDS
CTCAATAACAGCAGCAGCAG+TGG 0.545949 8.1:+16511065 None:intergenic
ATAACATGATTAGCAACTCC+TGG 0.549528 8.1:+16510080 None:intergenic
AGTAAAGAGGTTAATTGTGG+TGG 0.552977 8.1:-16513424 MS.gene061735:CDS
TGATTCCAAGAGTTTAGAAG+AGG 0.553393 8.1:-16513335 MS.gene061735:CDS
CTCATGGGAAAATATGGGTA+GGG 0.554839 8.1:+16509933 None:intergenic
GAAAGGCGGCCTCGTGTTAG+GGG 0.555000 8.1:-16509723 MS.gene061735:CDS
GGTCTGTAAACAAATACTCA+TGG 0.558038 8.1:+16509917 None:intergenic
TGTGGACGCACATGTGGAGA+AGG 0.558944 8.1:-16511945 MS.gene061735:CDS
GTCTGTAAACAAATACTCAT+GGG 0.559531 8.1:+16509918 None:intergenic
TGTTTGTATTGGCATCACTA+GGG 0.561104 8.1:+16511358 None:intergenic
GTCATCCCAGTTGCATGAGG+TGG 0.561134 8.1:+16509957 None:intergenic
CAATTGTTGTCAAGTGCTTG+AGG 0.561695 8.1:-16512023 MS.gene061735:CDS
TAAATTTGTGGACGCACATG+TGG 0.562177 8.1:-16511951 MS.gene061735:CDS
ACTCATGGGAAAATATGGGT+AGG 0.564653 8.1:+16509932 None:intergenic
TTGGGGAAGTTGATTGTACT+GGG 0.566596 8.1:+16509983 None:intergenic
CTATTTGTAACTCTGTTGCA+CGG 0.566787 8.1:+16510720 None:intergenic
ATCCTTAGGAGAGGAATCCC+TGG 0.570852 8.1:+16509596 None:intergenic
ATCGGAAACAACTTGCAGAA+AGG 0.571293 8.1:-16509740 MS.gene061735:CDS
TTCATCCTCATCGTCATAGT+CGG 0.573949 8.1:+16509641 None:intergenic
AACAACAATGAAGAGCAACT+TGG 0.581389 8.1:-16510444 MS.gene061735:CDS
AGGAAGAAAACAATGACACC+AGG 0.582197 8.1:-16509614 MS.gene061735:CDS
AGGTTTCTACAGCTCATCCA+TGG 0.583579 8.1:-16509890 MS.gene061735:CDS
AGATGTTGAAGTACATAGCA+CGG 0.586911 8.1:-16512207 MS.gene061735:CDS
ATCAAGCATGTTCTCAACAA+GGG 0.587610 8.1:-16510364 MS.gene061735:CDS
CTTGGATGAACCACCCCTTG+TGG 0.587951 8.1:+16510173 None:intergenic
CTATGACGATGAGGATGAAG+AGG 0.588668 8.1:-16509637 MS.gene061735:CDS
GATGTTGAAGTACATAGCAC+GGG 0.593466 8.1:-16512206 MS.gene061735:CDS
GAAGTTGAATGGTATTAATG+TGG 0.594169 8.1:-16509688 MS.gene061735:CDS
TGTGAAACCTCCATTGATGC+AGG 0.598440 8.1:+16510224 None:intergenic
AACCTCCATTGATGCAGGTG+GGG 0.598604 8.1:+16510229 None:intergenic
CAAACTTCAGAAAAGTCGAT+AGG 0.608964 8.1:-16509836 MS.gene061735:CDS
AATTTCAATCCAACAAGAAG+AGG 0.609391 8.1:-16511244 MS.gene061735:intron
ATCCTCATCGTCATAGTCGG+TGG 0.610990 8.1:+16509644 None:intergenic
GGTGTCATCCCAGTTGCATG+AGG 0.611782 8.1:+16509954 None:intergenic
AATGTCCACCTCATGCAACT+GGG 0.619079 8.1:-16509962 MS.gene061735:CDS
AGTGATGTCTGCACAAGATG+AGG 0.622148 8.1:-16512049 MS.gene061735:CDS
CGGTACATTACTAGTGAGGG+TGG 0.622559 8.1:-16510396 MS.gene061735:CDS
AATCAAGCATGTTCTCAACA+AGG 0.623938 8.1:-16510365 MS.gene061735:CDS
TTCTCAAGCATCCTTAGGAG+AGG 0.624497 8.1:+16509587 None:intergenic
TTCAATCCAACAAGAACAGG+CGG 0.626266 8.1:-16511268 MS.gene061735:CDS
TCAAGCATGTTCTCAACAAG+GGG 0.626567 8.1:-16510363 MS.gene061735:CDS
ATGAACTAACATGACTCTGA+AGG 0.628622 8.1:+16510015 None:intergenic
GGTGGAAATAATAGCAAGAG+TGG 0.630214 8.1:-16513406 MS.gene061735:CDS
ATAATAGCAAGAGTGGTGAT+GGG 0.630687 8.1:-16513399 MS.gene061735:CDS
GTTGCATGAGGTGGACATTG+GGG 0.632474 8.1:+16509966 None:intergenic
ATAGCGGTACATTACTAGTG+AGG 0.638404 8.1:-16510400 MS.gene061735:CDS
CACAGGGAAGAACATCCACA+AGG 0.638565 8.1:-16510188 MS.gene061735:CDS
GGAAACAACTTGCAGAAAGG+CGG 0.642044 8.1:-16509737 MS.gene061735:CDS
GAAACCTCCATTGATGCAGG+TGG 0.645336 8.1:+16510227 None:intergenic
ATGAGCTGTAGAAACCTGAC+CGG 0.646047 8.1:+16509896 None:intergenic
GGAAGAACATCCACAAGGGG+TGG 0.646616 8.1:-16510183 MS.gene061735:CDS
TGACGATGAGGATGAAGAGG+AGG 0.647407 8.1:-16509634 MS.gene061735:CDS
GAACTTCCTAATGACGGCCA+TGG 0.652127 8.1:+16509873 None:intergenic
TACCCCACCTGCATCAATGG+AGG 0.653828 8.1:-16510231 MS.gene061735:CDS
AAACCTCCATTGATGCAGGT+GGG 0.653891 8.1:+16510228 None:intergenic
AGTGATTGACGCATTGAATG+CGG 0.661141 8.1:-16512290 MS.gene061735:CDS
ATTGACGCATTGAATGCGGA+AGG 0.662540 8.1:-16512286 MS.gene061735:CDS
GAAGTTGAACTTCCTAATGA+CGG 0.668884 8.1:+16509867 None:intergenic
TAGCGGTACATTACTAGTGA+GGG 0.670900 8.1:-16510399 MS.gene061735:CDS
TTACGTCTGTAAAATCTGCA+CGG 0.675024 8.1:-16512315 MS.gene061735:CDS
CAGGGAAGAACATCCACAAG+GGG 0.677957 8.1:-16510186 MS.gene061735:CDS
ATATATAGATATTATACTAG+CGG 0.684747 8.1:-16512260 MS.gene061735:CDS
ACAGCAAAATAGTAATCACA+GGG 0.685208 8.1:-16510204 MS.gene061735:CDS
CAAAAGAAGCAATAATCCCG+AGG 0.687400 8.1:-16511298 MS.gene061735:CDS
TTCCACCGACTATGACGATG+AGG 0.690682 8.1:-16509646 MS.gene061735:CDS
ACAGGGAAGAACATCCACAA+GGG 0.694566 8.1:-16510187 MS.gene061735:CDS
GGAAGAAAACAATGACACCA+GGG 0.694692 8.1:-16509613 MS.gene061735:CDS
CATGTGGAGAAGGAGACGCA+TGG 0.700199 8.1:-16511935 MS.gene061735:intron
ATTGCTCATTCTGACAATAG+CGG 0.712115 8.1:-16510416 MS.gene061735:CDS
TGTGTCATCAGAGAAGAAGG+TGG 0.715634 8.1:+16511332 None:intergenic
ACAAACTGTCCCCTAACACG+AGG 0.728956 8.1:+16509714 None:intergenic
TTTGTAACTCTGTTGCACGG+CGG 0.729169 8.1:+16510723 None:intergenic
TCTCAACAGAGAAAGAATCG+GGG 0.738856 8.1:+16510261 None:intergenic
CAAGCATGTTCTCAACAAGG+GGG 0.755371 8.1:-16510362 MS.gene061735:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAAATATATAAATAATGT+TGG - chr8.1:16511299-16511318 MS.gene061735:CDS 10.0%
!! TGAAATATATAAATAATGTT+GGG - chr8.1:16511300-16511319 MS.gene061735:CDS 10.0%
!! AAAATAATGAAAATGACAAT+GGG + chr8.1:16512478-16512497 None:intergenic 15.0%
!! AATCGAAGTTAATAATAATT+TGG + chr8.1:16512165-16512184 None:intergenic 15.0%
!! ATATATAGATATTATACTAG+CGG - chr8.1:16510784-16510803 MS.gene061735:intron 15.0%
!! GAAATATATAAATAATGTTG+GGG - chr8.1:16511301-16511320 MS.gene061735:CDS 15.0%
!!! AATCTGATTTTAATTGAAAA+TGG + chr8.1:16511242-16511261 None:intergenic 15.0%
!!! TTTAGTTCTTTATTTTCATA+AGG - chr8.1:16510897-16510916 MS.gene061735:intron 15.0%
!! AACTAAAGCTTACTTATAAT+AGG + chr8.1:16510884-16510903 None:intergenic 20.0%
!! ACAAAATTATGTAAAATCAG+AGG + chr8.1:16512502-16512521 None:intergenic 20.0%
!! ACATAAGTTCTTATATGATA+AGG - chr8.1:16510184-16510203 MS.gene061735:CDS 20.0%
!! AGAACAGAATTAAGTAAATA+AGG + chr8.1:16510254-16510273 None:intergenic 20.0%
!! ATAGCTTATATATTCTCAAA+TGG - chr8.1:16511569-16511588 MS.gene061735:intron 20.0%
!! CAAAATAATGAAAATGACAA+TGG + chr8.1:16512479-16512498 None:intergenic 20.0%
!! CATAAGTTCTTATATGATAA+GGG - chr8.1:16510185-16510204 MS.gene061735:CDS 20.0%
!! CTAATGAACTATTAAATTTG+TGG - chr8.1:16511081-16511100 MS.gene061735:intron 20.0%
!! GAAAATAACTTTACATGTAA+TGG - chr8.1:16510586-16510605 MS.gene061735:intron 20.0%
!! GATATGATAAAACAGAAAAT+TGG + chr8.1:16512217-16512236 None:intergenic 20.0%
!! TAAGTAAATAAGGTCATATT+TGG + chr8.1:16510244-16510263 None:intergenic 20.0%
!! TCAAATAAGTCAATCTATAT+AGG + chr8.1:16509978-16509997 None:intergenic 20.0%
!! TGTATTGTACTACATATTTA+AGG - chr8.1:16511139-16511158 MS.gene061735:intron 20.0%
!! TTAAAAAATGAAGCTATAGA+AGG + chr8.1:16512383-16512402 None:intergenic 20.0%
!!! TTTGTAAAACATTTTTCATC+AGG - chr8.1:16512517-16512536 MS.gene061735:intron 20.0%
! AAACTATCCTATAAAGCTTA+TGG + chr8.1:16510093-16510112 None:intergenic 25.0%
! AAATACTCATGGGAAAATAT+GGG + chr8.1:16513119-16513138 None:intergenic 25.0%
! AAGCCAGATTAAAATTTCAT+CGG - chr8.1:16509918-16509937 MS.gene061735:CDS 25.0%
! AATAGTTCATTAGTACGTAA+AGG + chr8.1:16511074-16511093 None:intergenic 25.0%
! ATTCACATAGCCAAAAAATA+AGG + chr8.1:16509785-16509804 None:intergenic 25.0%
! ATTGTATTCTTCAAAGGAAA+AGG + chr8.1:16512082-16512101 None:intergenic 25.0%
! CTTATTAATTGCTCTTATCA+GGG - chr8.1:16509743-16509762 MS.gene061735:CDS 25.0%
! GCTTAATTAAGTCTAACTTA+TGG + chr8.1:16510399-16510418 None:intergenic 25.0%
! GCTTACTTATAATAGGAATT+CGG + chr8.1:16510877-16510896 None:intergenic 25.0%
! TACTACATATTTAAGGATGT+TGG - chr8.1:16511146-16511165 MS.gene061735:intron 25.0%
! TATCTATTATTATGACAGCT+TGG - chr8.1:16511612-16511631 MS.gene061735:intron 25.0%
! TATTCTGATACATGAACATA+TGG + chr8.1:16512011-16512030 None:intergenic 25.0%
! TCATATATTGCTTTCCAAAA+AGG - chr8.1:16511886-16511905 MS.gene061735:intron 25.0%
! TCTTATTAATTGCTCTTATC+AGG - chr8.1:16509742-16509761 MS.gene061735:CDS 25.0%
! TGATGAATTTAGAAAACTCA+AGG + chr8.1:16509839-16509858 None:intergenic 25.0%
!! AAAAGGCATATCAATTATGA+TGG - chr8.1:16511903-16511922 MS.gene061735:intron 25.0%
!! AAAATGGATTTACATTCATC+GGG + chr8.1:16511226-16511245 None:intergenic 25.0%
!! AGTAAATTTTATTCAGCTTG+AGG + chr8.1:16512445-16512464 None:intergenic 25.0%
!! CTCTGAAGATATATAAACTA+TGG - chr8.1:16510688-16510707 MS.gene061735:intron 25.0%
!! CTTATTTCCATAAGCTTTAT+AGG - chr8.1:16510083-16510102 MS.gene061735:CDS 25.0%
!! GAAAATGGATTTACATTCAT+CGG + chr8.1:16511227-16511246 None:intergenic 25.0%
!! TCATAATTGATATGCCTTTT+TGG + chr8.1:16511903-16511922 None:intergenic 25.0%
!! TTTACTGTTGCTTATTATAC+AGG - chr8.1:16512282-16512301 MS.gene061735:CDS 25.0%
!!! ATCTTCATTCCCTTTTTTAT+TGG + chr8.1:16510821-16510840 None:intergenic 25.0%
!!! ATGAACAAAAGCACTTAATT+AGG + chr8.1:16512260-16512279 None:intergenic 25.0%
!!! CTTGAATTATGATTTTGATC+TGG - chr8.1:16511653-16511672 MS.gene061735:intron 25.0%
!!! TATTGATTTGAACAGTAAAG+AGG - chr8.1:16509607-16509626 MS.gene061735:CDS 25.0%
!!! TGTAAAGTTATTTTCGTTCT+TGG + chr8.1:16510581-16510600 None:intergenic 25.0%
!!! TGTTGACTTTAATATTAGCA+TGG - chr8.1:16511854-16511873 MS.gene061735:intron 25.0%
AAAAACCTCTTCTAAACTCT+TGG + chr8.1:16509717-16509736 None:intergenic 30.0%
AAAATGACTTCAGTCTAGAA+GGG - chr8.1:16512408-16512427 MS.gene061735:intron 30.0%
AAAATTTCATCGGGCTAAAT+TGG - chr8.1:16509928-16509947 MS.gene061735:CDS 30.0%
AAAGAATCAAACTAGAGTAG+AGG + chr8.1:16511337-16511356 None:intergenic 30.0%
AACAGTAAAGAGGTTAATTG+TGG - chr8.1:16509617-16509636 MS.gene061735:CDS 30.0%
AAGAATCAAACTAGAGTAGA+GGG + chr8.1:16511336-16511355 None:intergenic 30.0%
AAGACATGAAAATGGAGAAA+CGG - chr8.1:16512717-16512736 MS.gene061735:intron 30.0%
AATTTCAATCCAACAAGAAC+AGG - chr8.1:16511773-16511792 MS.gene061735:intron 30.0%
AATTTCAATCCAACAAGAAG+AGG - chr8.1:16511800-16511819 MS.gene061735:intron 30.0%
ACAGCAAAATAGTAATCACA+GGG - chr8.1:16512840-16512859 MS.gene061735:intron 30.0%
ACATAGCCAAAAAATAAGGA+AGG + chr8.1:16509781-16509800 None:intergenic 30.0%
ACATGAAACATTATTCCAAG+AGG - chr8.1:16510361-16510380 MS.gene061735:CDS 30.0%
AGAAAATTAGGTACGTCAAT+CGG - chr8.1:16513286-16513305 MS.gene061735:intron 30.0%
AGCCAGATTAAAATTTCATC+GGG - chr8.1:16509919-16509938 MS.gene061735:CDS 30.0%
AGTATAACTCTTGTTCGATT+AGG - chr8.1:16509860-16509879 MS.gene061735:CDS 30.0%
AGTTGACCTTCCAATAAAAA+AGG - chr8.1:16510808-16510827 MS.gene061735:intron 30.0%
ATAATACGGATAAGCATGTT+GGG + chr8.1:16512941-16512960 None:intergenic 30.0%
ATATAAGCTATGCATTGCAT+TGG + chr8.1:16511560-16511579 None:intergenic 30.0%
ATGAGTATTTGTTTACAGAC+CGG - chr8.1:16513129-16513148 MS.gene061735:intron 30.0%
CAAATACTCATGGGAAAATA+TGG + chr8.1:16513120-16513139 None:intergenic 30.0%
CACGAAATTGTATTCTTCAA+AGG + chr8.1:16512088-16512107 None:intergenic 30.0%
CATAGCCAAAAAATAAGGAA+GGG + chr8.1:16509780-16509799 None:intergenic 30.0%
CGTGTAACACAAGATTAAAA+GGG + chr8.1:16511481-16511500 None:intergenic 30.0%
GAAGTTGAATGGTATTAATG+TGG - chr8.1:16513356-16513375 MS.gene061735:CDS 30.0%
GACCTTATAAGACATGAAAA+TGG - chr8.1:16512709-16512728 MS.gene061735:intron 30.0%
GCATATCAATTATGATGGTA+TGG - chr8.1:16511908-16511927 MS.gene061735:intron 30.0%
GGTATTAATGTGGATCTAAA+TGG - chr8.1:16513366-16513385 MS.gene061735:CDS 30.0%
GGTGAAGTTTAAGAACAAAT+GGG + chr8.1:16511205-16511224 None:intergenic 30.0%
GTCTGTAAACAAATACTCAT+GGG + chr8.1:16513129-16513148 None:intergenic 30.0%
GTGTAACACAAGATTAAAAG+GGG + chr8.1:16511480-16511499 None:intergenic 30.0%
GTTGACCTTCCAATAAAAAA+GGG - chr8.1:16510809-16510828 MS.gene061735:intron 30.0%
GTTGCTTCGATAAGAAAATT+AGG - chr8.1:16513274-16513293 MS.gene061735:intron 30.0%
TATAATACGGATAAGCATGT+TGG + chr8.1:16512942-16512961 None:intergenic 30.0%
TCATTGATAGGAGTAAAGTT+AGG - chr8.1:16509669-16509688 MS.gene061735:CDS 30.0%
TGCTTTGACATAAGTGAAAA+AGG + chr8.1:16512581-16512600 None:intergenic 30.0%
TGGATAAACAGCTTAGATAA+GGG + chr8.1:16510224-16510243 None:intergenic 30.0%
TGTTCACAAAAAATCCAGAT+CGG + chr8.1:16511401-16511420 None:intergenic 30.0%
TTCTTGGAATTGAAAAGGAA+GGG + chr8.1:16510565-16510584 None:intergenic 30.0%
TTGGATAAACAGCTTAGATA+AGG + chr8.1:16510225-16510244 None:intergenic 30.0%
TTTCGTTCTTGGAATTGAAA+AGG + chr8.1:16510570-16510589 None:intergenic 30.0%
! CTCCATTTTCATGTCTTATA+AGG + chr8.1:16512714-16512733 None:intergenic 30.0%
! GGAACTTGTAAGACTATTTT+AGG - chr8.1:16510918-16510937 MS.gene061735:intron 30.0%
!! GTTACAAATAGTAGCTTTGA+TGG - chr8.1:16512336-16512355 MS.gene061735:intron 30.0%
!!! ATTCACCCTTCCTTATTTTT+TGG - chr8.1:16509772-16509791 MS.gene061735:CDS 30.0%
!!! TAACTCACGTATACTTTTTC+AGG - chr8.1:16511947-16511966 MS.gene061735:CDS 30.0%
!!! TCATTCCCTTTTTTATTGGA+AGG + chr8.1:16510817-16510836 None:intergenic 30.0%
AAACATGCGTATGCATCAAA+CGG + chr8.1:16510330-16510349 None:intergenic 35.0%
AACAACAATGAAGAGCAACT+TGG - chr8.1:16512600-16512619 MS.gene061735:intron 35.0%
AAGAAGAGTGAACTCAGAAT+TGG - chr8.1:16512544-16512563 MS.gene061735:intron 35.0%
AATAAGCTGAAAACAGCTCA+TGG + chr8.1:16510069-16510088 None:intergenic 35.0%
AATAATAGCAAGAGTGGTGA+TGG - chr8.1:16509644-16509663 MS.gene061735:CDS 35.0%
AATCAAGCATGTTCTCAACA+AGG - chr8.1:16512679-16512698 MS.gene061735:intron 35.0%
ACAGAAAATTGGCTACTAGA+AGG + chr8.1:16512206-16512225 None:intergenic 35.0%
AGAATCAAACTAGAGTAGAG+GGG + chr8.1:16511335-16511354 None:intergenic 35.0%
AGACAAGGAAGGATAAGATA+AGG + chr8.1:16511427-16511446 None:intergenic 35.0%
AGATGTTGAAGTACATAGCA+CGG - chr8.1:16510837-16510856 MS.gene061735:intron 35.0%
AGCATAACCACATGTGATAT+TGG - chr8.1:16510009-16510028 MS.gene061735:CDS 35.0%
AGTAAAGAGGTTAATTGTGG+TGG - chr8.1:16509620-16509639 MS.gene061735:CDS 35.0%
AGTTAGTACATGTGAATGTG+TGG + chr8.1:16511275-16511294 None:intergenic 35.0%
ATAACATGATTAGCAACTCC+TGG + chr8.1:16512967-16512986 None:intergenic 35.0%
ATAATAGCAAGAGTGGTGAT+GGG - chr8.1:16509645-16509664 MS.gene061735:CDS 35.0%
ATCAAGCATGTTCTCAACAA+GGG - chr8.1:16512680-16512699 MS.gene061735:intron 35.0%
ATGAACTAACATGACTCTGA+AGG + chr8.1:16513032-16513051 None:intergenic 35.0%
ATTAGTGATCGAAGAAGAAC+AGG - chr8.1:16512050-16512069 MS.gene061735:CDS 35.0%
ATTCTGAGTTCACTCTTCTT+CGG + chr8.1:16512544-16512563 None:intergenic 35.0%
ATTGCACAAGTTCATACTTC+TGG + chr8.1:16510517-16510536 None:intergenic 35.0%
ATTGCTCATTCTGACAATAG+CGG - chr8.1:16512628-16512647 MS.gene061735:intron 35.0%
CAAACTTCAGAAAAGTCGAT+AGG - chr8.1:16513208-16513227 MS.gene061735:intron 35.0%
CAATGTAATGTGATGTGCAA+TGG + chr8.1:16511525-16511544 None:intergenic 35.0%
CACAGCAAAATAGTAATCAC+AGG - chr8.1:16512839-16512858 MS.gene061735:intron 35.0%
CATCCAAGAAATGGAAGTTA+TGG - chr8.1:16512886-16512905 MS.gene061735:intron 35.0%
CATTAAGTTCTTCCTATTCG+TGG - chr8.1:16510612-16510631 MS.gene061735:intron 35.0%
CTATTTGTAACTCTGTTGCA+CGG + chr8.1:16512327-16512346 None:intergenic 35.0%
GAAAATGACTTCAGTCTAGA+AGG - chr8.1:16512407-16512426 MS.gene061735:intron 35.0%
GAACCATAACTTCCATTTCT+TGG + chr8.1:16512892-16512911 None:intergenic 35.0%
GAAGTTGAACTTCCTAATGA+CGG + chr8.1:16513180-16513199 None:intergenic 35.0%
GAGGTGAATGTTAATTGCTT+TGG - chr8.1:16511819-16511838 MS.gene061735:intron 35.0%
GCGTGTAACACAAGATTAAA+AGG + chr8.1:16511482-16511501 None:intergenic 35.0%
GGGTGAAGTTTAAGAACAAA+TGG + chr8.1:16511206-16511225 None:intergenic 35.0%
GGTCTGTAAACAAATACTCA+TGG + chr8.1:16513130-16513149 None:intergenic 35.0%
GTTCTTGGAATTGAAAAGGA+AGG + chr8.1:16510566-16510585 None:intergenic 35.0%
TAACATTCACCTCTTCTTGT+TGG + chr8.1:16511812-16511831 None:intergenic 35.0%
TAATGTGATGTGCAATGGAA+TGG + chr8.1:16511520-16511539 None:intergenic 35.0%
TAGGTAAGTCATCTTGTGAA+TGG + chr8.1:16512750-16512769 None:intergenic 35.0%
TCTCTCAACAGAGAAAGAAT+CGG + chr8.1:16512788-16512807 None:intergenic 35.0%
TGATTCCAAGAGTTTAGAAG+AGG - chr8.1:16509709-16509728 MS.gene061735:CDS 35.0%
TTACGTCTGTAAAATCTGCA+CGG - chr8.1:16510729-16510748 MS.gene061735:CDS 35.0%
TTGCACAAGTTCATACTTCT+GGG + chr8.1:16510516-16510535 None:intergenic 35.0%
! AAATTGGTGCTTTCTTGTTG+TGG - chr8.1:16509944-16509963 MS.gene061735:CDS 35.0%
! ATATTGGTTGAGAGAGCTTA+TGG - chr8.1:16510025-16510044 MS.gene061735:CDS 35.0%
! GCTTACTTCAACTTTGAGAA+TGG - chr8.1:16510434-16510453 MS.gene061735:CDS 35.0%
! TGTTTGTATTGGCATCACTA+GGG + chr8.1:16511689-16511708 None:intergenic 35.0%
!! AGCCCGATGAAATTTTAATC+TGG + chr8.1:16509924-16509943 None:intergenic 35.0%
AAATTTCCGCCTGTTCTTGT+TGG + chr8.1:16511785-16511804 None:intergenic 40.0%
AACATGCGTATGCATCAAAC+GGG + chr8.1:16510329-16510348 None:intergenic 40.0%
AACGTACAAGAAGACAAGGA+AGG + chr8.1:16511438-16511457 None:intergenic 40.0%
AAGTATTTGTGCTCATTGGC+TGG - chr8.1:16510280-16510299 MS.gene061735:CDS 40.0%
AAGTTGTCTAGTGCAACTAC+AGG + chr8.1:16512126-16512145 None:intergenic 40.0%
ACACAAGATTAAAAGGGGCA+AGG + chr8.1:16511475-16511494 None:intergenic 40.0%
ACTCATGGGAAAATATGGGT+AGG + chr8.1:16513115-16513134 None:intergenic 40.0%
AGATCGGAAAAGAAGCGAAA+TGG + chr8.1:16511385-16511404 None:intergenic 40.0%
AGGAAGAAAACAATGACACC+AGG - chr8.1:16513430-16513449 MS.gene061735:CDS 40.0%
AGTGATTGACGCATTGAATG+CGG - chr8.1:16510754-16510773 MS.gene061735:intron 40.0%
ATAGCGGTACATTACTAGTG+AGG - chr8.1:16512644-16512663 MS.gene061735:intron 40.0%
ATCGGAAACAACTTGCAGAA+AGG - chr8.1:16513304-16513323 MS.gene061735:CDS 40.0%
CAAAAGAAGCAATAATCCCG+AGG - chr8.1:16511746-16511765 MS.gene061735:intron 40.0%
CAATTGTTGTCAAGTGCTTG+AGG - chr8.1:16511021-16511040 MS.gene061735:CDS 40.0%
CAGTTTGTCAGGAAGTTGAA+TGG - chr8.1:16513345-16513364 MS.gene061735:CDS 40.0%
CCAAAACGTACAAGAAGACA+AGG + chr8.1:16511442-16511461 None:intergenic 40.0%
CTCATGGGAAAATATGGGTA+GGG + chr8.1:16513114-16513133 None:intergenic 40.0%
CTCTCAACAGAGAAAGAATC+GGG + chr8.1:16512787-16512806 None:intergenic 40.0%
CTCTCAACCAATATCACATG+TGG + chr8.1:16510019-16510038 None:intergenic 40.0%
CTTCTTCGATCACTAATTCC+AGG + chr8.1:16512047-16512066 None:intergenic 40.0%
GACTTAATTAAGCCTGTGCA+AGG - chr8.1:16510406-16510425 MS.gene061735:CDS 40.0%
GATGTTGAAGTACATAGCAC+GGG - chr8.1:16510838-16510857 MS.gene061735:intron 40.0%
GCAACTCCTGGCATATAATA+CGG + chr8.1:16512955-16512974 None:intergenic 40.0%
GCCAATGAGCACAAATACTT+AGG + chr8.1:16510280-16510299 None:intergenic 40.0%
GCCTAAGTATTTGTGCTCAT+TGG - chr8.1:16510276-16510295 MS.gene061735:CDS 40.0%
GCTTATCCGTATTATATGCC+AGG - chr8.1:16512946-16512965 MS.gene061735:intron 40.0%
GGAAGAAAACAATGACACCA+GGG - chr8.1:16513431-16513450 MS.gene061735:CDS 40.0%
GGTGGAAATAATAGCAAGAG+TGG - chr8.1:16509638-16509657 MS.gene061735:CDS 40.0%
TAAATTTGTGGACGCACATG+TGG - chr8.1:16511093-16511112 MS.gene061735:intron 40.0%
TAGCGGTACATTACTAGTGA+GGG - chr8.1:16512645-16512664 MS.gene061735:intron 40.0%
TATTTGTTTACAGACCGGTC+AGG - chr8.1:16513134-16513153 MS.gene061735:intron 40.0%
TCAAGCATGTTCTCAACAAG+GGG - chr8.1:16512681-16512700 MS.gene061735:intron 40.0%
TCACTAATTCCAGGTACATC+AGG + chr8.1:16512038-16512057 None:intergenic 40.0%
TCTCAACAGAGAAAGAATCG+GGG + chr8.1:16512786-16512805 None:intergenic 40.0%
TGCATACGCATGTTTGTTCA+AGG - chr8.1:16510335-16510354 MS.gene061735:CDS 40.0%
TGGAAGCTAAATACTCCTCT+TGG + chr8.1:16510379-16510398 None:intergenic 40.0%
TTCAATCCAACAAGAACAGG+CGG - chr8.1:16511776-16511795 MS.gene061735:intron 40.0%
TTCATCCTCATCGTCATAGT+CGG + chr8.1:16513406-16513425 None:intergenic 40.0%
TTGGATTGAAATTCCAGCCT+CGG + chr8.1:16511766-16511785 None:intergenic 40.0%
! ATTGGGGAAGTTGATTGTAC+TGG + chr8.1:16513065-16513084 None:intergenic 40.0%
! ATTTCGCTTCTTTTCCGATC+TGG - chr8.1:16511384-16511403 MS.gene061735:CDS 40.0%
! CCTTGTCTTCTTGTACGTTT+TGG - chr8.1:16511439-16511458 MS.gene061735:intron 40.0%
! CTCGGGATTATTGCTTCTTT+TGG + chr8.1:16511748-16511767 None:intergenic 40.0%
! CTGTTTGTATTGGCATCACT+AGG + chr8.1:16511690-16511709 None:intergenic 40.0%
! TAACATGACTCTGAAGGTTC+TGG + chr8.1:16513026-16513045 None:intergenic 40.0%
! TGCTTTCTTGTTGTGGAGTT+GGG - chr8.1:16509951-16509970 MS.gene061735:CDS 40.0%
! TTGGGGAAGTTGATTGTACT+GGG + chr8.1:16513064-16513083 None:intergenic 40.0%
AAACCTCCATTGATGCAGGT+GGG + chr8.1:16512819-16512838 None:intergenic 45.0%
AATGTCCACCTCATGCAACT+GGG - chr8.1:16513082-16513101 MS.gene061735:intron 45.0%
ACAGGGAAGAACATCCACAA+GGG - chr8.1:16512857-16512876 MS.gene061735:intron 45.0%
ACTAGAAGGCGACAAAGGAT+AGG + chr8.1:16512192-16512211 None:intergenic 45.0%
AGGTTTCTACAGCTCATCCA+TGG - chr8.1:16513154-16513173 MS.gene061735:intron 45.0%
AGTAAGCAAGTTCCTTGCAC+AGG + chr8.1:16510421-16510440 None:intergenic 45.0%
AGTGATGTCTGCACAAGATG+AGG - chr8.1:16510995-16511014 MS.gene061735:CDS 45.0%
AGTTGCATGAGGTGGACATT+GGG + chr8.1:16513082-16513101 None:intergenic 45.0%
ATGAGCTGTAGAAACCTGAC+CGG + chr8.1:16513151-16513170 None:intergenic 45.0%
ATTGACGCATTGAATGCGGA+AGG - chr8.1:16510758-16510777 MS.gene061735:intron 45.0%
CAAGCATGTTCTCAACAAGG+GGG - chr8.1:16512682-16512701 MS.gene061735:intron 45.0%
CTATGACGATGAGGATGAAG+AGG - chr8.1:16513407-16513426 MS.gene061735:CDS 45.0%
GAAGGTGGCACTGTTTGTAT+TGG + chr8.1:16511700-16511719 None:intergenic 45.0%
GAATATCAGCCTGATGTACC+TGG - chr8.1:16512026-16512045 MS.gene061735:CDS 45.0%
GGAAACAACTTGCAGAAAGG+CGG - chr8.1:16513307-16513326 MS.gene061735:CDS 45.0%
GGAATTGAAAAGGAAGGGAG+AGG + chr8.1:16510560-16510579 None:intergenic 45.0%
GTCTGTGTCATCAGAGAAGA+AGG + chr8.1:16511718-16511737 None:intergenic 45.0%
TCGGGGATACTATTGCTTCT+AGG + chr8.1:16512769-16512788 None:intergenic 45.0%
TCTTGTGTTACACGCGCATT+TGG - chr8.1:16511487-16511506 MS.gene061735:intron 45.0%
TGGATTGAAATTCCAGCCTC+GGG + chr8.1:16511765-16511784 None:intergenic 45.0%
TGTGAAACCTCCATTGATGC+AGG + chr8.1:16512823-16512842 None:intergenic 45.0%
TGTGTCATCAGAGAAGAAGG+TGG + chr8.1:16511715-16511734 None:intergenic 45.0%
TTTGTAACTCTGTTGCACGG+CGG + chr8.1:16512324-16512343 None:intergenic 45.0%
! GCTTTCTTGTTGTGGAGTTG+GGG - chr8.1:16509952-16509971 MS.gene061735:CDS 45.0%
! GTGCTTTCTTGTTGTGGAGT+TGG - chr8.1:16509950-16509969 MS.gene061735:CDS 45.0%
! TGTGCAATATTTTCCCCCTG+TGG - chr8.1:16510529-16510548 MS.gene061735:intron 45.0%
!! GTGGTGATGGGTTCATTGAT+AGG - chr8.1:16509657-16509676 MS.gene061735:CDS 45.0%
!! TGGTTCTGAGCTCAACCAAT+CGG - chr8.1:16512906-16512925 MS.gene061735:intron 45.0%
!!! TATTTCTTATTTTTTATTTT+TGG - chr8.1:16510969-16510988 MS.gene061735:intron 5.0%
AACCTCCATTGATGCAGGTG+GGG + chr8.1:16512818-16512837 None:intergenic 50.0%
ACAAACTGTCCCCTAACACG+AGG + chr8.1:16513333-16513352 None:intergenic 50.0%
AGAAGCAATAATCCCGAGGC+TGG - chr8.1:16511750-16511769 MS.gene061735:intron 50.0%
ATCCTCATCGTCATAGTCGG+TGG + chr8.1:16513403-16513422 None:intergenic 50.0%
ATCCTTAGGAGAGGAATCCC+TGG + chr8.1:16513451-16513470 None:intergenic 50.0%
CAATGTCCACCTCATGCAAC+TGG - chr8.1:16513081-16513100 MS.gene061735:intron 50.0%
CACAGGGAAGAACATCCACA+AGG - chr8.1:16512856-16512875 MS.gene061735:intron 50.0%
CACTGCTGCTGCTGTTATTG+AGG - chr8.1:16511980-16511999 MS.gene061735:CDS 50.0%
CAGGGAAGAACATCCACAAG+GGG - chr8.1:16512858-16512877 MS.gene061735:intron 50.0%
CAGTTGCATGAGGTGGACAT+TGG + chr8.1:16513083-16513102 None:intergenic 50.0%
CGGTACATTACTAGTGAGGG+TGG - chr8.1:16512648-16512667 MS.gene061735:intron 50.0%
CTCAATAACAGCAGCAGCAG+TGG + chr8.1:16511982-16512001 None:intergenic 50.0%
GAAACCTCCATTGATGCAGG+TGG + chr8.1:16512820-16512839 None:intergenic 50.0%
GAACTTCCTAATGACGGCCA+TGG + chr8.1:16513174-16513193 None:intergenic 50.0%
GGGGTGGTTCATCCAAGAAA+TGG - chr8.1:16512877-16512896 MS.gene061735:intron 50.0%
GTGTTAGGGGACAGTTTGTC+AGG - chr8.1:16513334-16513353 MS.gene061735:CDS 50.0%
GTTGCATGAGGTGGACATTG+GGG + chr8.1:16513081-16513100 None:intergenic 50.0%
GTTGTCAAGTGCTTGAGGCT+CGG - chr8.1:16511026-16511045 MS.gene061735:CDS 50.0%
TACGGATAAGCATGTTGGGC+AGG + chr8.1:16512937-16512956 None:intergenic 50.0%
TCCTATTCGTGGATGCAGTC+TGG - chr8.1:16510623-16510642 MS.gene061735:intron 50.0%
TGACGATGAGGATGAAGAGG+AGG - chr8.1:16513410-16513429 MS.gene061735:CDS 50.0%
TGGCTACTAGAAGGCGACAA+AGG + chr8.1:16512197-16512216 None:intergenic 50.0%
! TTCCACCGACTATGACGATG+AGG - chr8.1:16513398-16513417 MS.gene061735:CDS 50.0%
!! GGTTCTGAGCTCAACCAATC+GGG - chr8.1:16512907-16512926 MS.gene061735:intron 50.0%
AAGGGAGAGGTTGCTCCACA+GGG + chr8.1:16510547-16510566 None:intergenic 55.0%
AGAAAGGCGGCCTCGTGTTA+GGG - chr8.1:16513320-16513339 MS.gene061735:CDS 55.0%
CACCAGGGATTCCTCTCCTA+AGG - chr8.1:16513446-16513465 MS.gene061735:CDS 55.0%
CATGTGGAGAAGGAGACGCA+TGG - chr8.1:16511109-16511128 MS.gene061735:intron 55.0%
CTTGGATGAACCACCCCTTG+TGG + chr8.1:16512874-16512893 None:intergenic 55.0%
GCCAGACTGCATCCACGAAT+AGG + chr8.1:16510627-16510646 None:intergenic 55.0%
GCTCATCCATGGCCGTCATT+AGG - chr8.1:16513165-16513184 MS.gene061735:intron 55.0%
GGAAGAACATCCACAAGGGG+TGG - chr8.1:16512861-16512880 MS.gene061735:intron 55.0%
GTCATCCCAGTTGCATGAGG+TGG + chr8.1:16513090-16513109 None:intergenic 55.0%
GTGTACCCCACCTGCATCAA+TGG - chr8.1:16512810-16512829 MS.gene061735:intron 55.0%
TACCCCACCTGCATCAATGG+AGG - chr8.1:16512813-16512832 MS.gene061735:intron 55.0%
TCGTCATAGTCGGTGGAAGC+AGG + chr8.1:16513396-16513415 None:intergenic 55.0%
TGTGGACGCACATGTGGAGA+AGG - chr8.1:16511099-16511118 MS.gene061735:intron 55.0%
! GGTGTCATCCCAGTTGCATG+AGG + chr8.1:16513093-16513112 None:intergenic 55.0%
AGGGAGAGGTTGCTCCACAG+GGG + chr8.1:16510546-16510565 None:intergenic 60.0%
CAGAAAGGCGGCCTCGTGTT+AGG - chr8.1:16513319-16513338 MS.gene061735:CDS 60.0%
GAAAGGCGGCCTCGTGTTAG+GGG - chr8.1:16513321-16513340 MS.gene061735:CDS 60.0%
GAAGGGAGAGGTTGCTCCAC+AGG + chr8.1:16510548-16510567 None:intergenic 60.0%
GGGAGAGGTTGCTCCACAGG+GGG + chr8.1:16510545-16510564 None:intergenic 65.0%
GTTGGGCAGGCATGCCCGAT+TGG + chr8.1:16512924-16512943 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 16509590 16513476 16509590 ID=MS.gene061735
chr8.1 mRNA 16509590 16513476 16509590 ID=MS.gene061735.t1;Parent=MS.gene061735
chr8.1 exon 16513302 16513476 16513302 ID=MS.gene061735.t1.exon1;Parent=MS.gene061735.t1
chr8.1 CDS 16513302 16513476 16513302 ID=cds.MS.gene061735.t1;Parent=MS.gene061735.t1
chr8.1 exon 16512176 16512337 16512176 ID=MS.gene061735.t1.exon2;Parent=MS.gene061735.t1
chr8.1 CDS 16512176 16512337 16512176 ID=cds.MS.gene061735.t1;Parent=MS.gene061735.t1
chr8.1 exon 16511936 16512069 16511936 ID=MS.gene061735.t1.exon3;Parent=MS.gene061735.t1
chr8.1 CDS 16511936 16512069 16511936 ID=cds.MS.gene061735.t1;Parent=MS.gene061735.t1
chr8.1 exon 16511245 16511436 16511245 ID=MS.gene061735.t1.exon4;Parent=MS.gene061735.t1
chr8.1 CDS 16511245 16511436 16511245 ID=cds.MS.gene061735.t1;Parent=MS.gene061735.t1
chr8.1 exon 16510995 16511097 16510995 ID=MS.gene061735.t1.exon5;Parent=MS.gene061735.t1
chr8.1 CDS 16510995 16511097 16510995 ID=cds.MS.gene061735.t1;Parent=MS.gene061735.t1
chr8.1 exon 16510709 16510762 16510709 ID=MS.gene061735.t1.exon6;Parent=MS.gene061735.t1
chr8.1 CDS 16510709 16510762 16510709 ID=cds.MS.gene061735.t1;Parent=MS.gene061735.t1
chr8.1 exon 16509590 16510527 16509590 ID=MS.gene061735.t1.exon7;Parent=MS.gene061735.t1
chr8.1 CDS 16509590 16510527 16509590 ID=cds.MS.gene061735.t1;Parent=MS.gene061735.t1
Gene Sequence

>MS.gene061735

ATGGATACTGAAGAACTTATTGATTTGAACAGTAAAGAGGTTAATTGTGGTGGAAATAATAGCAAGAGTGGTGATGGGTTCATTGATAGGAGTAAAGTTAGGATTTTGCTGTGTGATAGTGATTCCAAGAGTTTAGAAGAGGTTTTTACTCTTCTTATTAATTGCTCTTATCAGGGTAAGAAATTCACCCTTCCTTATTTTTTGGCTATGTGAATTTTTTTGCTGTGTGAATTTTTTGTGCTAAATCCTTGAGTTTTCTAAATTCATCAGAGTATAACTCTTGTTCGATTAGGTAGAAAAAAAACATGTTAGCATATTGTTATTTTGTAAGCCAGATTAAAATTTCATCGGGCTAAATTGGTGCTTTCTTGTTGTGGAGTTGGGGCCTATATAGATTGACTTATTTGAACTTATCTTCTAGCATAACCACATGTGATATTGGTTGAGAGAGCTTATGGAAACAACTGATATCGTGTCCATGAGCTGTTTTCAGCTTATTTCCATAAGCTTTATAGGATAGTTTATGAAAACAATTTATAGCTTGTATGAGAATAATTTGACTTTATTTTATCTTTTGTTATAGAGATAGTTTATACATAAGTTCTTATATGATAAGGGCTTATAGCATAATCCCTTATCTAAGCTGTTTATCCAAATATGACCTTATTTACTTAATTCTGTTCTCTGCCTAAGTATTTGTGCTCATTGGCTGGTTGAAATTATCAAATTTGTTTTTCCCGTTTGATGCATACGCATGTTTGTTCAAGGTGTACATGAAACATTATTCCAAGAGGAGTATTTAGCTTCCATAAGTTAGACTTAATTAAGCCTGTGCAAGGAACTTGCTTACTTCAACTTTGAGAATGGATTTTTCTTGAGTTTTCATGAATTTCGAAATTTAATTTTATCTGTAAGAATCAACACCCAGAAGTATGAACTTGTGCAATATTTTCCCCCTGTGGAGCAACCTCTCCCTTCCTTTTCAATTCCAAGAACGAAAATAACTTTACATGTAATGGTTTCATTAAGTTCTTCCTATTCGTGGATGCAGTCTGGCTTGTTTGTATGATTTAATGTTTCTGCGATTCTTCAAATATTCTCTGAAGATATATAAACTATGGATTTTTCATGCTTCACAGTTACGTCTGTAAAATCTGCACGGCAAGTGATTGACGCATTGAATGCGGAAGGACAATATATAGATATTATACTAGCGGAAGTTGACCTTCCAATAAAAAAGGGAATGAAGATGTTGAAGTACATAGCACGGGATAAAGAACTGCGCCGAATTCCTATTATAAGTAAGCTTTAGTTCTTTATTTTCATAAGGAACTTGTAAGACTATTTTAGGCAAATTATATTATGTTCTTTGTCAATGTTATTTCTTATTTTTTATTTTTGGTTTAGTGATGTCTGCACAAGATGAGGTATCAATTGTTGTCAAGTGCTTGAGGCTCGGAGCAGCAGACTATCTAGTAAAGCCTTTACGTACTAATGAACTATTAAATTTGTGGACGCACATGTGGAGAAGGAGACGCATGGTATGTAATGTATTGTACTACATATTTAAGGATGTTGGCTACTTGTGTATATTTGTACTATTTTTTCTTTTCCCATTTGTTCTTAAACTTCACCCGATGAATGTAAATCCATTTTCAATTAAAATCAGATTTCATGCACAACCACACATTCACATGTACTAACTATAAATGAAATATATAAATAATGTTGGGGTAGCATCTCCCCTCTACTCTAGTTTGATTCTTTAATATGCATGTGCAATTGTGTATCTCCATTTCGCTTCTTTTCCGATCTGGATTTTTTGTGAACATTTCCTTATCTTATCCTTCCTTGTCTTCTTGTACGTTTTGGTCTGTTGTATCCTTGCCCCTTTTAATCTTGTGTTACACGCGCATTTGGCATATCTCCATTCCATTGCACATCACATTACATTGTTCTTTAAATTTCCAATGCAATGCATAGCTTATATATTCTCAAATGGAACTTGAAATGTTTTTTTTTTATCTATTATTATGACAGCTTGGACTTATAGAGAACAACATCTTGAATTATGATTTTGATCTGGTAGCATCAGACCCTAGTGATGCCAATACAAACAGTGCCACCTTCTTCTCTGATGACACAGACGACAAGTCCAAAAGAAGCAATAATCCCGAGGCTGGAATTTCAATCCAACAAGAACAGGCGGAAATTTCAATCCAACAAGAAGAGGTGAATGTTAATTGCTTTGGAAATTATTTCAGTGTTGACTTTAATATTAGCATGGTTCACACTCTCATATATTGCTTTCCAAAAAGGCATATCAATTATGATGGTATGGTCGAGATTATGCTATATAACTCACGTATACTTTTTCAGGCTAGTATTGCCACTGCTGCTGCTGTTATTGAGGAGCATCCATATGTTCATGTATCAGAATATCAGCCTGATGTACCTGGAATTAGTGATCGAAGAAGAACAGGTACACTCCTTTTCCTTTGAAGAATACAATTTCGTGTACAGCTAGTTGTTTCCTGTAGTTGCACTAGACAACTTGTATGATAGATATATGCCAAATTATTATTAACTTCGATTTTTTCCTATCCTTTGTCGCCTTCTAGTAGCCAATTTTCTGTTTTATCATATCTAATATTCAATTTCAAGAAACCTAATTAAGTGCTTTTGTTCATGCTTTACTGTTGCTTATTATACAGGTTCTTCAGCACAGCATCCGCCGTGCAACAGAGTTACAAATAGTAGCTTTGATGGTAGTTTGCACATCTTATTTTTCCTTCTATAGCTTCATTTTTTAATTATGAAAATGACTTCAGTCTAGAAGGGATAGTTAAGTTCCTCAAGCTGAATAAAATTTACTGATGTGAGAACCCATTGTCATTTTCATTATTTTGCCTCTGATTTTACATAATTTTGTAAAACATTTTTCATCAGGTCCGAAGAAGAGTGAACTCAGAATTGGAGAATCATCAGCCTTTTTCACTTATGTCAAAGCAACAACAATGAAGAGCAACTTGGAAGAGATTGCTCATTCTGACAATAGCGGTACATTACTAGTGAGGGTGGAAGATATGAATCAAGCATGTTCTCAACAAGGGGGTAATGACCTTATAAGACATGAAAATGGAGAAACGGTTGAAAGCCATTCACAAGATGACTTACCTAGAAGCAATAGTATCCCCGATTCTTTCTCTGTTGAGAGATCGTGTACCCCACCTGCATCAATGGAGGTTTCACAGCAAAATAGTAATCACAGGGAAGAACATCCACAAGGGGTGGTTCATCCAAGAAATGGAAGTTATGGTTCTGAGCTCAACCAATCGGGCATGCCTGCCCAACATGCTTATCCGTATTATATGCCAGGAGTTGCTAATCATGTTATGATGCAGTCATCAACACATGTGTATCAAAAGAATATCCAGAACCTTCAGAGTCATGTTAGTTCATCTATGATTTCCCAGTACAATCAACTTCCCCAATGTCCACCTCATGCAACTGGGATGACACCCTACCCATATTTTCCCATGAGTATTTGTTTACAGACCGGTCAGGTTTCTACAGCTCATCCATGGCCGTCATTAGGAAGTTCAACTTCATGTGAAGCAAACTTCAGAAAAGTCGATAGGAGAGAAGCAGCATTGATGAAGTTTAGACAGAAAAGAAAAGAGCGTTGCTTCGATAAGAAAATTAGGTACGTCAATCGGAAACAACTTGCAGAAAGGCGGCCTCGTGTTAGGGGACAGTTTGTCAGGAAGTTGAATGGTATTAATGTGGATCTAAATGGACAACCTGCTTCCACCGACTATGACGATGAGGATGAAGAGGAGGAAGAAAACAATGACACCAGGGATTCCTCTCCTAAGGATGCTTGA
Protein sequence

>MS.gene061735.t1

MDTEELIDLNSKEVNCGGNNSKSGDGFIDRSKVRILLCDSDSKSLEEVFTLLINCSYQVTSVKSARQVIDALNAEGQYIDIILAEVDLPIKKGMKMLKYIARDKELRRIPIIMMSAQDEVSIVVKCLRLGAADYLVKPLRTNELLNLWTHMWRRRRMLGLIENNILNYDFDLVASDPSDANTNSATFFSDDTDDKSKRSNNPEAGISIQQEQAEISIQQEEASIATAAAVIEEHPYVHVSEYQPDVPGISDRRRTGSSAQHPPCNRVTNSSFDGPKKSELRIGESSAFFTYVKATTMKSNLEEIAHSDNSGTLLVRVEDMNQACSQQGGNDLIRHENGETVESHSQDDLPRSNSIPDSFSVERSCTPPASMEVSQQNSNHREEHPQGVVHPRNGSYGSELNQSGMPAQHAYPYYMPGVANHVMMQSSTHVYQKNIQNLQSHVSSSMISQYNQLPQCPPHATGMTPYPYFPMSICLQTGQVSTAHPWPSLGSSTSCEANFRKVDRREAALMKFRQKRKERCFDKKIRYVNRKQLAERRPRVRGQFVRKLNGINVDLNGQPASTDYDDEDEEEEENNDTRDSSPKDA