Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059557.t1 | KEH27201.1 | 82.7 | 52 | 9 | 0 | 1 | 52 | 348 | 399 | 1.10E-12 | 81.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059557.t1 | A0A072UCL4 | 82.7 | 52 | 9 | 0 | 1 | 52 | 348 | 399 | 8.3e-13 | 81.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059557 | MS.gene062407 | 0.801924 | 7.16E-49 | -1.69E-46 |
| MS.gene059557 | MS.gene06269 | 0.802926 | 4.45E-49 | -1.69E-46 |
| MS.gene059557 | MS.gene06701 | 0.812411 | 4.30E-51 | -1.69E-46 |
| MS.gene059557 | MS.gene31283 | 0.800358 | 1.50E-48 | -1.69E-46 |
| MS.gene059557 | MS.gene41121 | 0.803723 | 3.04E-49 | -1.69E-46 |
| MS.gene059557 | MS.gene52525 | 0.810286 | 1.24E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059557.t1 | MTR_6g088700 | 82.692 | 52 | 9 | 0 | 1 | 52 | 348 | 399 | 1.70e-21 | 84.7 |
| MS.gene059557.t1 | MTR_4g092430 | 71.154 | 52 | 15 | 0 | 1 | 52 | 356 | 407 | 3.33e-18 | 75.9 |
| MS.gene059557.t1 | MTR_4g092430 | 71.154 | 52 | 15 | 0 | 1 | 52 | 376 | 427 | 3.40e-18 | 75.9 |
| MS.gene059557.t1 | MTR_4g092390 | 65.385 | 52 | 18 | 0 | 1 | 52 | 123 | 174 | 3.99e-16 | 67.8 |
| MS.gene059557.t1 | MTR_8g085310 | 67.308 | 52 | 17 | 0 | 1 | 52 | 248 | 299 | 2.45e-15 | 67.8 |
| MS.gene059557.t1 | MTR_4g051560 | 65.385 | 52 | 18 | 0 | 1 | 52 | 268 | 319 | 3.11e-15 | 67.4 |
| MS.gene059557.t1 | MTR_4g092980 | 65.385 | 52 | 18 | 0 | 1 | 52 | 348 | 399 | 6.48e-15 | 66.6 |
| MS.gene059557.t1 | MTR_1g094760 | 63.462 | 52 | 19 | 0 | 1 | 52 | 339 | 390 | 9.53e-15 | 66.2 |
| MS.gene059557.t1 | MTR_4g090940 | 63.462 | 52 | 19 | 0 | 1 | 52 | 491 | 542 | 1.49e-14 | 65.5 |
| MS.gene059557.t1 | MTR_4g092360 | 65.306 | 49 | 17 | 0 | 1 | 49 | 369 | 417 | 4.30e-14 | 64.3 |
| MS.gene059557.t1 | MTR_4g092440 | 59.615 | 52 | 21 | 0 | 1 | 52 | 378 | 429 | 1.12e-13 | 63.2 |
| MS.gene059557.t1 | MTR_4g092440 | 59.615 | 52 | 21 | 0 | 1 | 52 | 378 | 429 | 1.18e-13 | 63.2 |
| MS.gene059557.t1 | MTR_4g133790 | 59.615 | 52 | 20 | 1 | 1 | 52 | 199 | 249 | 1.71e-12 | 59.3 |
| MS.gene059557.t1 | MTR_4g051480 | 55.769 | 52 | 23 | 0 | 1 | 52 | 363 | 414 | 2.95e-12 | 58.9 |
| MS.gene059557.t1 | MTR_4g092510 | 60.870 | 46 | 18 | 0 | 7 | 52 | 409 | 454 | 6.23e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTGATGTGATGTTAACTT+TGG | 0.455218 | 6.4:+61853704 | None:intergenic |
| TTCCGTACCTGATGGGATAG+TGG | 0.486142 | 6.4:-61853754 | MS.gene059557:CDS |
| AAGAAGTCCACTATCCCATC+AGG | 0.520187 | 6.4:+61853747 | None:intergenic |
| GTACGAAAAGCATTTAAAGC+GGG | 0.539045 | 6.4:-61853790 | MS.gene059557:CDS |
| AGCAGCAGCTTTCTTTAACA+TGG | 0.540497 | 6.4:+61853836 | None:intergenic |
| GAACCACCTTCCGTACCTGA+TGG | 0.549208 | 6.4:-61853762 | MS.gene059557:CDS |
| GTCCACTATCCCATCAGGTA+CGG | 0.559841 | 6.4:+61853752 | None:intergenic |
| AACCACCTTCCGTACCTGAT+GGG | 0.562342 | 6.4:-61853761 | MS.gene059557:CDS |
| ATCCCATCAGGTACGGAAGG+TGG | 0.633481 | 6.4:+61853759 | None:intergenic |
| ACTATCCCATCAGGTACGGA+AGG | 0.645921 | 6.4:+61853756 | None:intergenic |
| AGTACGAAAAGCATTTAAAG+CGG | 0.652727 | 6.4:-61853791 | MS.gene059557:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCTGATGTGATGTTAACTT+TGG | + | chr6.4:61853831-61853850 | None:intergenic | 30.0% | |
| ! | CTTTAAATGCTTTTCGTACT+TGG | + | chr6.4:61853741-61853760 | None:intergenic | 30.0% |
| !! | AGTACGAAAAGCATTTAAAG+CGG | - | chr6.4:61853741-61853760 | MS.gene059557:CDS | 30.0% |
| !! | GTACGAAAAGCATTTAAAGC+GGG | - | chr6.4:61853742-61853761 | MS.gene059557:CDS | 35.0% |
| AAGAAGTCCACTATCCCATC+AGG | + | chr6.4:61853788-61853807 | None:intergenic | 45.0% | |
| AACCACCTTCCGTACCTGAT+GGG | - | chr6.4:61853771-61853790 | MS.gene059557:CDS | 50.0% | |
| ACTATCCCATCAGGTACGGA+AGG | + | chr6.4:61853779-61853798 | None:intergenic | 50.0% | |
| GTCCACTATCCCATCAGGTA+CGG | + | chr6.4:61853783-61853802 | None:intergenic | 50.0% | |
| TTCCGTACCTGATGGGATAG+TGG | - | chr6.4:61853778-61853797 | MS.gene059557:CDS | 50.0% | |
| ATCCCATCAGGTACGGAAGG+TGG | + | chr6.4:61853776-61853795 | None:intergenic | 55.0% | |
| GAACCACCTTCCGTACCTGA+TGG | - | chr6.4:61853770-61853789 | MS.gene059557:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 61853698 | 61853856 | 61853698 | ID=MS.gene059557 |
| chr6.4 | mRNA | 61853698 | 61853856 | 61853698 | ID=MS.gene059557.t1;Parent=MS.gene059557 |
| chr6.4 | exon | 61853698 | 61853856 | 61853698 | ID=MS.gene059557.t1.exon1;Parent=MS.gene059557.t1 |
| chr6.4 | CDS | 61853698 | 61853856 | 61853698 | ID=cds.MS.gene059557.t1;Parent=MS.gene059557.t1 |
| Gene Sequence |
| Protein sequence |
