Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062428.t1 | XP_003621904.1 | 92.9 | 562 | 22 | 1 | 1 | 544 | 1 | 562 | 6.10E-290 | 1006.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062428.t1 | Q9MAU3 | 60.9 | 550 | 208 | 3 | 1 | 544 | 1 | 549 | 1.4e-181 | 637.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062428.t1 | G7KXV7 | 92.9 | 562 | 22 | 1 | 1 | 544 | 1 | 562 | 4.4e-290 | 1006.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene062428 | MS.gene26816 | PPI |
| MS.gene030125 | MS.gene062428 | PPI |
| MS.gene062428 | MS.gene061229 | PPI |
| MS.gene68598 | MS.gene062428 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062428.t1 | MTR_7g024860 | 94.662 | 562 | 12 | 1 | 1 | 544 | 1 | 562 | 0.0 | 1085 |
| MS.gene062428.t1 | MTR_7g024867 | 94.662 | 562 | 12 | 1 | 1 | 544 | 1 | 562 | 0.0 | 1085 |
| MS.gene062428.t1 | MTR_3g055240 | 57.721 | 544 | 196 | 5 | 1 | 544 | 1 | 510 | 0.0 | 596 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062428.t1 | AT1G04950 | 63.357 | 554 | 188 | 6 | 1 | 544 | 1 | 549 | 0.0 | 686 |
| MS.gene062428.t1 | AT1G04950 | 63.357 | 554 | 188 | 6 | 1 | 544 | 1 | 549 | 0.0 | 686 |
| MS.gene062428.t1 | AT1G04950 | 63.357 | 554 | 188 | 6 | 1 | 544 | 1 | 549 | 0.0 | 686 |
| MS.gene062428.t1 | AT1G54360 | 47.059 | 561 | 243 | 7 | 3 | 544 | 1 | 526 | 1.06e-160 | 468 |
| MS.gene062428.t1 | AT1G54360 | 47.059 | 561 | 243 | 7 | 3 | 544 | 15 | 540 | 1.43e-160 | 468 |
Find 105 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGTTCATCTTTCCAAATA+TGG | 0.136550 | 7.3:+75278890 | None:intergenic |
| CATGAAGCTTGGCGTGTTTA+TGG | 0.154260 | 7.3:-75280090 | MS.gene062428:CDS |
| CTTCTGTCCTAGCCCATATT+TGG | 0.203384 | 7.3:-75278903 | MS.gene062428:CDS |
| AACGTTGTTGAATGCATTCT+TGG | 0.218762 | 7.3:-75280998 | MS.gene062428:CDS |
| TTCTTATGTCAGCCAATATA+TGG | 0.220354 | 7.3:-75283370 | MS.gene062428:intron |
| GTAAGTTTGGCTACTGATTC+AGG | 0.252357 | 7.3:-75282430 | MS.gene062428:CDS |
| TGTTGTGTCATTGCCTTCTT+TGG | 0.257279 | 7.3:+75280973 | None:intergenic |
| CGTATATATGCTTCAAGATT+TGG | 0.258387 | 7.3:+75280165 | None:intergenic |
| TAAAGAAGCATTAGTAAGTT+TGG | 0.260790 | 7.3:-75282443 | MS.gene062428:CDS |
| TAACCTGCCTTGTTGCTAAA+AGG | 0.298000 | 7.3:-75281786 | MS.gene062428:CDS |
| ATAACTTCGATTGTTTCTTT+AGG | 0.299682 | 7.3:+75285960 | None:intergenic |
| CTAGATAAGACATGCTTAAC+AGG | 0.311507 | 7.3:+75282613 | None:intergenic |
| GCTACAAAGCATGTGAAATA+AGG | 0.321309 | 7.3:+75282391 | None:intergenic |
| TGGAAAGATGAACTGAATTC+AGG | 0.326248 | 7.3:-75278883 | MS.gene062428:CDS |
| AAGTTATTGCTCAGAGTATT+GGG | 0.333702 | 7.3:-75285944 | MS.gene062428:CDS |
| AACTTACATAAGGTTCAATA+TGG | 0.335073 | 7.3:+75282186 | None:intergenic |
| ACAACACTATGGTGCAATTC+AGG | 0.341769 | 7.3:-75280956 | MS.gene062428:CDS |
| CAGTTCATCTTTCCAAATAT+GGG | 0.342840 | 7.3:+75278891 | None:intergenic |
| CAAGGCAAGCCCTATGGAAT+TGG | 0.343736 | 7.3:-75279145 | MS.gene062428:CDS |
| TGTCTTATCTAGAGAGCTTC+AGG | 0.353561 | 7.3:-75282602 | MS.gene062428:intron |
| TCTTTAAAGAGAACCGAATC+AGG | 0.355917 | 7.3:+75282460 | None:intergenic |
| ACAGGTAATTTGATATCAAC+TGG | 0.361767 | 7.3:+75282631 | None:intergenic |
| CCAGATGCAAATCCATATAT+TGG | 0.371634 | 7.3:+75283358 | None:intergenic |
| GCGTGTTTATGGAGCCTTGC+TGG | 0.371996 | 7.3:-75280079 | MS.gene062428:intron |
| GGGAGAGGAGATGGAAAAGC+GGG | 0.385964 | 7.3:+75279913 | None:intergenic |
| CCTGAAAATGCTCCTGTAGA+TGG | 0.391023 | 7.3:-75283085 | MS.gene062428:intron |
| ACATCATTGGTTGAGCTGTT+TGG | 0.393677 | 7.3:-75278850 | MS.gene062428:CDS |
| TGGAGATGTTAGTGTAGTTC+AGG | 0.394478 | 7.3:-75279088 | MS.gene062428:CDS |
| GAAGTTATTGCTCAGAGTAT+TGG | 0.399221 | 7.3:-75285945 | MS.gene062428:CDS |
| TGTGTCTCAAGAAGCATCTC+TGG | 0.409556 | 7.3:+75280132 | None:intergenic |
| TGCCTTGTTGCTAAAAGGTT+GGG | 0.413976 | 7.3:-75281781 | MS.gene062428:CDS |
| GGCTGCTGCTCCAATTCCAT+AGG | 0.419772 | 7.3:+75279135 | None:intergenic |
| CTCCTCTCCCTCATACTGTC+TGG | 0.432250 | 7.3:-75279900 | MS.gene062428:CDS |
| ACAAGGCAGGTTACAATTGA+TGG | 0.435760 | 7.3:+75281796 | None:intergenic |
| AGCTTTGAATGAGCCTGATT+CGG | 0.436229 | 7.3:-75282473 | MS.gene062428:CDS |
| GCAGTATCAAGGGGAGCTTT+TGG | 0.439198 | 7.3:+75283154 | None:intergenic |
| CAGTATCTACTTTCTTCAAA+GGG | 0.439281 | 7.3:+75279112 | None:intergenic |
| TGTTTCATTCAGCGTGCTGC+AGG | 0.440328 | 7.3:-75279967 | MS.gene062428:intron |
| CCAATATATGGATTTGCATC+TGG | 0.443044 | 7.3:-75283358 | MS.gene062428:CDS |
| CTGCCTTGTTGCTAAAAGGT+TGG | 0.459360 | 7.3:-75281782 | MS.gene062428:CDS |
| ACAGTATGAGGGAGAGGAGA+TGG | 0.459983 | 7.3:+75279904 | None:intergenic |
| GCAACAGAAAGCGCAACATC+AGG | 0.464025 | 7.3:+75285906 | None:intergenic |
| TATGGTGCAATTCAGGGGTT+AGG | 0.464972 | 7.3:-75280949 | MS.gene062428:CDS |
| CATCTTTCCAAATATGGGCT+AGG | 0.469577 | 7.3:+75278896 | None:intergenic |
| ATTGGTGCAGCGTCATCCTC+TGG | 0.470899 | 7.3:-75279000 | MS.gene062428:CDS |
| CTGGTCTTCCAGACAGTATG+AGG | 0.477347 | 7.3:+75279892 | None:intergenic |
| CAAGATTTGGCAGCAAAAGA+AGG | 0.477595 | 7.3:+75280178 | None:intergenic |
| AGGACGTGTCCTGACATCAT+TGG | 0.478074 | 7.3:-75278863 | MS.gene062428:CDS |
| CGCATACGGTATTCAACATC+AGG | 0.480045 | 7.3:+75285882 | None:intergenic |
| GACAGGTAAGTGCAGTATCA+AGG | 0.484319 | 7.3:+75283143 | None:intergenic |
| TCAGTATCTACTTTCTTCAA+AGG | 0.486930 | 7.3:+75279111 | None:intergenic |
| ATACTGCACTTACCTGTCAC+TGG | 0.488327 | 7.3:-75283138 | MS.gene062428:CDS |
| TTCCAGACAGTATGAGGGAG+AGG | 0.490867 | 7.3:+75279898 | None:intergenic |
| ACAGGTAAGTGCAGTATCAA+GGG | 0.492623 | 7.3:+75283144 | None:intergenic |
| GTCTCCTCTTCCTTATGAGA+TGG | 0.492865 | 7.3:+75279054 | None:intergenic |
| AAATTCTTCACCATCTCATA+AGG | 0.493204 | 7.3:-75279064 | MS.gene062428:CDS |
| TTACAATTGATGGCATCAAC+TGG | 0.493457 | 7.3:+75281806 | None:intergenic |
| TTCGATGGCAAGCCAGTGAC+AGG | 0.496154 | 7.3:+75283126 | None:intergenic |
| TAAACGCAAGGCAAGCCCTA+TGG | 0.498294 | 7.3:-75279151 | MS.gene062428:CDS |
| AAGGCAATGACACAACACTA+TGG | 0.502589 | 7.3:-75280967 | MS.gene062428:CDS |
| TTGGGAACTTAGAGACTTCA+CGG | 0.503314 | 7.3:-75281737 | MS.gene062428:CDS |
| CAATCCTTTCAGGTTTCTCG+TGG | 0.508236 | 7.3:-75282278 | MS.gene062428:intron |
| AGGGAGAGGAGATGGAAAAG+CGG | 0.510840 | 7.3:+75279912 | None:intergenic |
| CAACACTATGGTGCAATTCA+GGG | 0.511881 | 7.3:-75280955 | MS.gene062428:CDS |
| GGTGAAGTTAGAAGCAGTGA+GGG | 0.512556 | 7.3:-75278949 | MS.gene062428:CDS |
| TGCATTCTTGGATCCAAAGA+AGG | 0.513153 | 7.3:-75280986 | MS.gene062428:CDS |
| GAGAGGGACAATACCACATT+GGG | 0.513459 | 7.3:+75280916 | None:intergenic |
| GTTAGATAGCTACCATCTAC+AGG | 0.515613 | 7.3:+75283073 | None:intergenic |
| CACATGCTTTGTAGCTGATG+AGG | 0.521153 | 7.3:-75282383 | MS.gene062428:intron |
| GCTGCTGCTCCAATTCCATA+GGG | 0.522208 | 7.3:+75279136 | None:intergenic |
| GCTATTAACAACTCGCATCA+AGG | 0.536109 | 7.3:+75282229 | None:intergenic |
| TGATTCAGGACTTCATCCAT+TGG | 0.539715 | 7.3:-75282416 | MS.gene062428:CDS |
| TGGTTGCATCAATATGCAAA+AGG | 0.541657 | 7.3:-75281708 | MS.gene062428:intron |
| TAGAGACTTCACGGCTAACC+TGG | 0.544251 | 7.3:-75281728 | MS.gene062428:CDS |
| CATGTGAAATAAGGAACCAA+TGG | 0.544256 | 7.3:+75282400 | None:intergenic |
| CCTGCATGATCTGACGCATA+CGG | 0.547929 | 7.3:+75285868 | None:intergenic |
| CGCATCAAGGCAAATAGAAG+TGG | 0.550878 | 7.3:+75282242 | None:intergenic |
| TTTATAGCAATCTACAGTCC+CGG | 0.553507 | 7.3:-75281029 | MS.gene062428:CDS |
| AAATGAAAAGACATGAAGCT+TGG | 0.554764 | 7.3:-75280101 | MS.gene062428:CDS |
| TTCACCATCTCATAAGGAAG+AGG | 0.558979 | 7.3:-75279058 | MS.gene062428:CDS |
| ACAGCTCAACCAATGATGTC+AGG | 0.559937 | 7.3:+75278854 | None:intergenic |
| TTGAAGAAAGTAGATACTGA+TGG | 0.560684 | 7.3:-75279108 | MS.gene062428:CDS |
| TAGATTGACAGACAACCATT+GGG | 0.562642 | 7.3:-75281755 | MS.gene062428:CDS |
| GTAGATTGACAGACAACCAT+TGG | 0.564706 | 7.3:-75281756 | MS.gene062428:CDS |
| AAGCAGTGAGGGTGATACTC+AGG | 0.570545 | 7.3:-75278938 | MS.gene062428:CDS |
| AGCAGGTTGCACTCCTTCGA+TGG | 0.571796 | 7.3:+75283111 | None:intergenic |
| TGTCACTGGCTTGCCATCGA+AGG | 0.581402 | 7.3:-75283124 | MS.gene062428:CDS |
| ATTCAGGGGTTAGGAGCACT+AGG | 0.597296 | 7.3:-75280940 | MS.gene062428:CDS |
| AGAAGTAAGAACACTTGCAC+TGG | 0.600577 | 7.3:+75279873 | None:intergenic |
| GGAGAGGGACAATACCACAT+TGG | 0.615478 | 7.3:+75280915 | None:intergenic |
| CCGTATGCGTCAGATCATGC+AGG | 0.616047 | 7.3:-75285868 | MS.gene062428:intron |
| TGATCATGAAGATAAACGCA+AGG | 0.624991 | 7.3:-75279163 | MS.gene062428:intron |
| AACACTATGGTGCAATTCAG+GGG | 0.625157 | 7.3:-75280954 | MS.gene062428:CDS |
| CTACGATTCAAACGAGCTGT+CGG | 0.629625 | 7.3:-75283328 | MS.gene062428:CDS |
| AGTATCTACTTTCTTCAAAG+GGG | 0.630790 | 7.3:+75279113 | None:intergenic |
| GTATCTACTTTCTTCAAAGG+GGG | 0.631481 | 7.3:+75279114 | None:intergenic |
| ATATATGGATTTGCATCTGG+TGG | 0.632207 | 7.3:-75283355 | MS.gene062428:CDS |
| GATAACATATCTTACCAGCA+AGG | 0.646319 | 7.3:+75280065 | None:intergenic |
| TGAAGTCTCTAAGTTCCCAA+TGG | 0.651064 | 7.3:+75281740 | None:intergenic |
| CAGGTAAGTGCAGTATCAAG+GGG | 0.654159 | 7.3:+75283145 | None:intergenic |
| TGGTCTTCCAGACAGTATGA+GGG | 0.668975 | 7.3:+75279893 | None:intergenic |
| ACAGCTCGTTTGAATCGTAG+AGG | 0.677708 | 7.3:+75283331 | None:intergenic |
| TGGTGAAGTTAGAAGCAGTG+AGG | 0.680296 | 7.3:-75278950 | MS.gene062428:CDS |
| AGGAGCACTAGGACCCAATG+TGG | 0.719832 | 7.3:-75280929 | MS.gene062428:intron |
| GGAGAGGAGATGGAAAAGCG+GGG | 0.719916 | 7.3:+75279914 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 75278798 | 75285994 | 75278798 | ID=MS.gene062428 |
| chr7.3 | mRNA | 75278798 | 75285994 | 75278798 | ID=MS.gene062428.t1;Parent=MS.gene062428 |
| chr7.3 | exon | 75285869 | 75285994 | 75285869 | ID=MS.gene062428.t1.exon1;Parent=MS.gene062428.t1 |
| chr7.3 | CDS | 75285869 | 75285994 | 75285869 | ID=cds.MS.gene062428.t1;Parent=MS.gene062428.t1 |
| chr7.3 | exon | 75284346 | 75284432 | 75284346 | ID=MS.gene062428.t1.exon2;Parent=MS.gene062428.t1 |
| chr7.3 | CDS | 75284346 | 75284432 | 75284346 | ID=cds.MS.gene062428.t1;Parent=MS.gene062428.t1 |
| chr7.3 | exon | 75283279 | 75283380 | 75283279 | ID=MS.gene062428.t1.exon3;Parent=MS.gene062428.t1 |
| chr7.3 | CDS | 75283279 | 75283380 | 75283279 | ID=cds.MS.gene062428.t1;Parent=MS.gene062428.t1 |
| chr7.3 | exon | 75283086 | 75283194 | 75283086 | ID=MS.gene062428.t1.exon4;Parent=MS.gene062428.t1 |
| chr7.3 | CDS | 75283086 | 75283194 | 75283086 | ID=cds.MS.gene062428.t1;Parent=MS.gene062428.t1 |
| chr7.3 | exon | 75282603 | 75282706 | 75282603 | ID=MS.gene062428.t1.exon5;Parent=MS.gene062428.t1 |
| chr7.3 | CDS | 75282603 | 75282706 | 75282603 | ID=cds.MS.gene062428.t1;Parent=MS.gene062428.t1 |
| chr7.3 | exon | 75282384 | 75282521 | 75282384 | ID=MS.gene062428.t1.exon6;Parent=MS.gene062428.t1 |
| chr7.3 | CDS | 75282384 | 75282521 | 75282384 | ID=cds.MS.gene062428.t1;Parent=MS.gene062428.t1 |
| chr7.3 | exon | 75282193 | 75282288 | 75282193 | ID=MS.gene062428.t1.exon7;Parent=MS.gene062428.t1 |
| chr7.3 | CDS | 75282193 | 75282288 | 75282193 | ID=cds.MS.gene062428.t1;Parent=MS.gene062428.t1 |
| chr7.3 | exon | 75281709 | 75281833 | 75281709 | ID=MS.gene062428.t1.exon8;Parent=MS.gene062428.t1 |
| chr7.3 | CDS | 75281709 | 75281833 | 75281709 | ID=cds.MS.gene062428.t1;Parent=MS.gene062428.t1 |
| chr7.3 | exon | 75280930 | 75281062 | 75280930 | ID=MS.gene062428.t1.exon9;Parent=MS.gene062428.t1 |
| chr7.3 | CDS | 75280930 | 75281062 | 75280930 | ID=cds.MS.gene062428.t1;Parent=MS.gene062428.t1 |
| chr7.3 | exon | 75280080 | 75280205 | 75280080 | ID=MS.gene062428.t1.exon10;Parent=MS.gene062428.t1 |
| chr7.3 | CDS | 75280080 | 75280205 | 75280080 | ID=cds.MS.gene062428.t1;Parent=MS.gene062428.t1 |
| chr7.3 | exon | 75279866 | 75279977 | 75279866 | ID=MS.gene062428.t1.exon11;Parent=MS.gene062428.t1 |
| chr7.3 | CDS | 75279866 | 75279977 | 75279866 | ID=cds.MS.gene062428.t1;Parent=MS.gene062428.t1 |
| chr7.3 | exon | 75278798 | 75279174 | 75278798 | ID=MS.gene062428.t1.exon12;Parent=MS.gene062428.t1 |
| chr7.3 | CDS | 75278798 | 75279174 | 75278798 | ID=cds.MS.gene062428.t1;Parent=MS.gene062428.t1 |
| Gene Sequence |
| Protein sequence |
