Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062525.t1 | XP_013445800.1 | 98.8 | 325 | 4 | 0 | 1 | 325 | 1 | 325 | 3.60E-189 | 670.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062525.t1 | P49730 | 83.0 | 329 | 52 | 1 | 1 | 325 | 1 | 329 | 3.9e-160 | 565.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062525.t1 | A0A072TRK2 | 98.8 | 325 | 4 | 0 | 1 | 325 | 1 | 325 | 2.6e-189 | 670.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05157 | MS.gene062525 | 0.802879 | 4.55E-49 | -1.69E-46 |
| MS.gene051720 | MS.gene062525 | 0.803422 | 3.51E-49 | -1.69E-46 |
| MS.gene054584 | MS.gene062525 | 0.803309 | 3.71E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene062525 | 0.813706 | 2.23E-51 | -1.69E-46 |
| MS.gene056169 | MS.gene062525 | 0.828392 | 9.23E-55 | -1.69E-46 |
| MS.gene060519 | MS.gene062525 | 0.809965 | 1.46E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene79484 | MS.gene062525 | PPI |
| MS.gene37310 | MS.gene062525 | PPI |
| MS.gene062525 | MS.gene034044 | PPI |
| MS.gene062525 | MS.gene034046 | PPI |
| MS.gene026793 | MS.gene062525 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062525.t1 | MTR_8g469010 | 98.769 | 325 | 4 | 0 | 1 | 325 | 1 | 325 | 0.0 | 672 |
| MS.gene062525.t1 | MTR_1g050472 | 93.030 | 330 | 18 | 1 | 1 | 325 | 1 | 330 | 0.0 | 634 |
| MS.gene062525.t1 | MTR_0268s0080 | 91.515 | 330 | 23 | 1 | 1 | 325 | 1 | 330 | 0.0 | 632 |
| MS.gene062525.t1 | MTR_7g029110 | 91.212 | 330 | 24 | 1 | 1 | 325 | 1 | 330 | 0.0 | 610 |
| MS.gene062525.t1 | MTR_4g117370 | 75.926 | 324 | 74 | 1 | 6 | 325 | 18 | 341 | 0.0 | 529 |
| MS.gene062525.t1 | MTR_6g005270 | 69.767 | 43 | 13 | 0 | 265 | 307 | 25 | 67 | 3.99e-14 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062525.t1 | AT3G27060 | 81.325 | 332 | 55 | 3 | 1 | 325 | 1 | 332 | 0.0 | 538 |
| MS.gene062525.t1 | AT3G23580 | 76.543 | 324 | 72 | 1 | 6 | 325 | 18 | 341 | 0.0 | 520 |
Find 54 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTTCTTTCATGAATCTTC+CGG | 0.295095 | 8.1:-44642248 | None:intergenic |
| TCAGAAGCGCGTGCGTTCTA+TGG | 0.296534 | 8.1:+44642277 | MS.gene062525:CDS |
| CGCTTCCTTGCGCGTTGGTT+GGG | 0.300830 | 8.1:+44642728 | MS.gene062525:CDS |
| GTTTGTTGCTGATAGGTTAT+TGG | 0.303774 | 8.1:+44642783 | MS.gene062525:CDS |
| GGAGCGAGAAGAGGTTCTTC+TGG | 0.345478 | 8.1:-44642013 | None:intergenic |
| GTTGCTTTCGCTTGTGTTGA+AGG | 0.362449 | 8.1:+44642484 | MS.gene062525:CDS |
| GCGCTTCCTTGCGCGTTGGT+TGG | 0.363506 | 8.1:+44642727 | MS.gene062525:CDS |
| AAGCTGATTGGGCTTTGAAA+TGG | 0.371966 | 8.1:+44642428 | MS.gene062525:CDS |
| GCCGTCGGATGCAGCGAAAA+AGG | 0.376281 | 8.1:-44642206 | None:intergenic |
| TTTGTTGCTGATAGGTTATT+GGG | 0.379609 | 8.1:+44642784 | MS.gene062525:CDS |
| ATGCAGAATCGGTCTGGGTT+TGG | 0.382220 | 8.1:-44642034 | None:intergenic |
| TGCTGATTATTGCTTTACTT+TGG | 0.386701 | 8.1:+44642942 | MS.gene062525:CDS |
| ATGTTCAGAATCCGTTTGAT+TGG | 0.390491 | 8.1:+44642833 | MS.gene062525:CDS |
| TGCGCGTTGGTTGGGATGAA+TGG | 0.417042 | 8.1:+44642736 | MS.gene062525:CDS |
| GGGAACATGCAGAATCGGTC+TGG | 0.418807 | 8.1:-44642040 | None:intergenic |
| GAGCGAGAAGAGGTTCTTCT+GGG | 0.439709 | 8.1:-44642012 | None:intergenic |
| TCAGAATCCGTTTGATTGGA+TGG | 0.451932 | 8.1:+44642837 | MS.gene062525:CDS |
| ATGGACTTGATTTCGCTTCA+AGG | 0.452863 | 8.1:+44642856 | MS.gene062525:CDS |
| TATTGCTAAGAAAGCTGATT+GGG | 0.465189 | 8.1:+44642417 | MS.gene062525:CDS |
| AATCGCTTATATACGTTTCG+AGG | 0.468396 | 8.1:-44642348 | None:intergenic |
| ACGCACGCGCTTCTGAGACT+TGG | 0.471778 | 8.1:-44642270 | None:intergenic |
| GATAGGTTATTGGGTGAACT+TGG | 0.489753 | 8.1:+44642793 | MS.gene062525:CDS |
| GGAACATGCAGAATCGGTCT+GGG | 0.489835 | 8.1:-44642039 | None:intergenic |
| ATCAAGTCCATCCAATCAAA+CGG | 0.492106 | 8.1:-44642844 | None:intergenic |
| ATATTGAGTTTGTTGCTGAT+AGG | 0.493329 | 8.1:+44642776 | MS.gene062525:CDS |
| GAGCTTATTTCACGTGATGA+AGG | 0.493446 | 8.1:+44642586 | MS.gene062525:CDS |
| TGATGCAGTTGAGATTGAAC+GGG | 0.498883 | 8.1:+44642690 | MS.gene062525:CDS |
| GGAGGAAAGATCGACTTCTT+CGG | 0.499480 | 8.1:-44642122 | None:intergenic |
| GGCGGTCCAGAACGAAGCTT+CGG | 0.504261 | 8.1:-44642101 | None:intergenic |
| TTATTGCTTTACTTTGGATG+AGG | 0.510830 | 8.1:+44642948 | MS.gene062525:CDS |
| TTTCCTCCGATCTTCAACAC+TGG | 0.515193 | 8.1:+44642137 | MS.gene062525:CDS |
| GTATTGCTAAGAAAGCTGAT+TGG | 0.515724 | 8.1:+44642416 | MS.gene062525:CDS |
| CAGAAAGCTTCTGTTATGAA+TGG | 0.519026 | 8.1:+44642916 | MS.gene062525:CDS |
| TGGACTTGATTTCGCTTCAA+GGG | 0.520384 | 8.1:+44642857 | MS.gene062525:CDS |
| GGATTGGGAACATGCAGAAT+CGG | 0.521690 | 8.1:-44642045 | None:intergenic |
| AGAGGTTCTTCTGGGAAAGA+AGG | 0.536510 | 8.1:-44642004 | None:intergenic |
| TGAAATAAGCTCGTTTGAGA+AGG | 0.538441 | 8.1:-44642575 | None:intergenic |
| CGATGCGCTTCCTTGCGCGT+TGG | 0.545347 | 8.1:+44642723 | MS.gene062525:CDS |
| GTTGTTCCAGTGTTGAAGAT+CGG | 0.557909 | 8.1:-44642143 | None:intergenic |
| AACGAAGTGGCGTTCGCCGT+CGG | 0.563379 | 8.1:-44642170 | None:intergenic |
| GTGATGCAGTTGAGATTGAA+CGG | 0.564180 | 8.1:+44642689 | MS.gene062525:CDS |
| TCTGGGTTTGGAGCGAGAAG+AGG | 0.577657 | 8.1:-44642022 | None:intergenic |
| TCGTTTGAGAAGGTTAATCC+CGG | 0.587906 | 8.1:-44642565 | None:intergenic |
| TTCATCCCAACCAACGCGCA+AGG | 0.598863 | 8.1:-44642733 | None:intergenic |
| CACTGGAACAACCTCACCGA+CGG | 0.611309 | 8.1:+44642154 | MS.gene062525:CDS |
| ATTGTCCTCGAAAACCTCGC+CGG | 0.613556 | 8.1:+44642229 | MS.gene062525:CDS |
| AGTGGCGTTCGCCGTCGGTG+AGG | 0.618653 | 8.1:-44642165 | None:intergenic |
| GTTCCAGTGTTGAAGATCGG+AGG | 0.622372 | 8.1:-44642140 | None:intergenic |
| CTTTCATGAATCTTCCGGCG+AGG | 0.631655 | 8.1:-44642243 | None:intergenic |
| CGAGGACATGGGAAACGAAG+TGG | 0.631743 | 8.1:-44642183 | None:intergenic |
| TCAGCTTTCTTAGCAATACA+CGG | 0.642160 | 8.1:-44642412 | None:intergenic |
| GAAACTGAGTACAGAGCGTG+TGG | 0.662428 | 8.1:+44642654 | MS.gene062525:CDS |
| AAAGCTTCTGTTATGAATGG+TGG | 0.674687 | 8.1:+44642919 | MS.gene062525:CDS |
| GGAAAGATCGACTTCTTCGG+CGG | 0.687608 | 8.1:-44642119 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATCCAATACCCAAAAATCT+GGG | + | chr8.1:44642063-44642082 | MS.gene062525:CDS | 30.0% | |
| GATTTCTTTCATGAATCTTC+CGG | - | chr8.1:44642251-44642270 | None:intergenic | 30.0% | |
| TATTGCTAAGAAAGCTGATT+GGG | + | chr8.1:44642417-44642436 | MS.gene062525:CDS | 30.0% | |
| TTATTGCTTTACTTTGGATG+AGG | + | chr8.1:44642948-44642967 | MS.gene062525:CDS | 30.0% | |
| TTTGTTGCTGATAGGTTATT+GGG | + | chr8.1:44642784-44642803 | MS.gene062525:CDS | 30.0% | |
| ! | GTTGAAGGAATTTTCTTTTC+AGG | + | chr8.1:44642499-44642518 | MS.gene062525:CDS | 30.0% |
| ! | TGCTGATTATTGCTTTACTT+TGG | + | chr8.1:44642942-44642961 | MS.gene062525:CDS | 30.0% |
| ! | TGTACATTTCCCAGATTTTT+GGG | - | chr8.1:44642075-44642094 | None:intergenic | 30.0% |
| ! | TTGTACATTTCCCAGATTTT+TGG | - | chr8.1:44642076-44642095 | None:intergenic | 30.0% |
| !! | ATATTGAGTTTGTTGCTGAT+AGG | + | chr8.1:44642776-44642795 | MS.gene062525:CDS | 30.0% |
| !!! | AGATTTTTGGGTATTGGATT+GGG | - | chr8.1:44642063-44642082 | None:intergenic | 30.0% |
| !!! | ATTTTCTGGTTGAAAAAACG+CGG | + | chr8.1:44642535-44642554 | MS.gene062525:CDS | 30.0% |
| AAAGCTTCTGTTATGAATGG+TGG | + | chr8.1:44642919-44642938 | MS.gene062525:CDS | 35.0% | |
| AATCGCTTATATACGTTTCG+AGG | - | chr8.1:44642351-44642370 | None:intergenic | 35.0% | |
| ATCAAGTCCATCCAATCAAA+CGG | - | chr8.1:44642847-44642866 | None:intergenic | 35.0% | |
| ATGTTCAGAATCCGTTTGAT+TGG | + | chr8.1:44642833-44642852 | MS.gene062525:CDS | 35.0% | |
| CAATCCAATACCCAAAAATC+TGG | + | chr8.1:44642062-44642081 | MS.gene062525:CDS | 35.0% | |
| CAGAAAGCTTCTGTTATGAA+TGG | + | chr8.1:44642916-44642935 | MS.gene062525:CDS | 35.0% | |
| GTATTGCTAAGAAAGCTGAT+TGG | + | chr8.1:44642416-44642435 | MS.gene062525:CDS | 35.0% | |
| TCAGCTTTCTTAGCAATACA+CGG | - | chr8.1:44642415-44642434 | None:intergenic | 35.0% | |
| TGAAATAAGCTCGTTTGAGA+AGG | - | chr8.1:44642578-44642597 | None:intergenic | 35.0% | |
| ! | GTTTGTTGCTGATAGGTTAT+TGG | + | chr8.1:44642783-44642802 | MS.gene062525:CDS | 35.0% |
| !!! | AATTTTTTTGAGAAGCGCGT+TGG | + | chr8.1:44642886-44642905 | MS.gene062525:CDS | 35.0% |
| !!! | ATTTTTTTGAGAAGCGCGTT+GGG | + | chr8.1:44642887-44642906 | MS.gene062525:CDS | 35.0% |
| !!! | CAGATTTTTGGGTATTGGAT+TGG | - | chr8.1:44642064-44642083 | None:intergenic | 35.0% |
| !!! | TTTCCCAGATTTTTGGGTAT+TGG | - | chr8.1:44642069-44642088 | None:intergenic | 35.0% |
| AAAAAACGCGGTTTAATGCC+GGG | + | chr8.1:44642547-44642566 | MS.gene062525:CDS | 40.0% | |
| ATGGACTTGATTTCGCTTCA+AGG | + | chr8.1:44642856-44642875 | MS.gene062525:CDS | 40.0% | |
| GAAAAAACGCGGTTTAATGC+CGG | + | chr8.1:44642546-44642565 | MS.gene062525:CDS | 40.0% | |
| GAGCTTATTTCACGTGATGA+AGG | + | chr8.1:44642586-44642605 | MS.gene062525:CDS | 40.0% | |
| GATAGGTTATTGGGTGAACT+TGG | + | chr8.1:44642793-44642812 | MS.gene062525:CDS | 40.0% | |
| GTGATGCAGTTGAGATTGAA+CGG | + | chr8.1:44642689-44642708 | MS.gene062525:CDS | 40.0% | |
| TCAGAATCCGTTTGATTGGA+TGG | + | chr8.1:44642837-44642856 | MS.gene062525:CDS | 40.0% | |
| TCGTTTGAGAAGGTTAATCC+CGG | - | chr8.1:44642568-44642587 | None:intergenic | 40.0% | |
| TGATGCAGTTGAGATTGAAC+GGG | + | chr8.1:44642690-44642709 | MS.gene062525:CDS | 40.0% | |
| TGGACTTGATTTCGCTTCAA+GGG | + | chr8.1:44642857-44642876 | MS.gene062525:CDS | 40.0% | |
| ! | GAATGTTTTCGATAGCGATC+TGG | - | chr8.1:44642306-44642325 | None:intergenic | 40.0% |
| ! | GTTGTTCCAGTGTTGAAGAT+CGG | - | chr8.1:44642146-44642165 | None:intergenic | 40.0% |
| !! | AAGCTGATTGGGCTTTGAAA+TGG | + | chr8.1:44642428-44642447 | MS.gene062525:CDS | 40.0% |
| !!! | TTTTTTTGAGAAGCGCGTTG+GGG | + | chr8.1:44642888-44642907 | MS.gene062525:CDS | 40.0% |
| AAAAAGCCGAAGCTTCGTTC+TGG | + | chr8.1:44642095-44642114 | MS.gene062525:CDS | 45.0% | |
| GGATTGGGAACATGCAGAAT+CGG | - | chr8.1:44642048-44642067 | None:intergenic | 45.0% | |
| TTTCCTCCGATCTTCAACAC+TGG | + | chr8.1:44642137-44642156 | MS.gene062525:CDS | 45.0% | |
| ! | AGAGGTTCTTCTGGGAAAGA+AGG | - | chr8.1:44642007-44642026 | None:intergenic | 45.0% |
| ! | GAAGCTTCTGTGCGATTTTC+TGG | + | chr8.1:44642521-44642540 | MS.gene062525:CDS | 45.0% |
| ! | GGAGGAAAGATCGACTTCTT+CGG | - | chr8.1:44642125-44642144 | None:intergenic | 45.0% |
| ! | GTTGCTTTCGCTTGTGTTGA+AGG | + | chr8.1:44642484-44642503 | MS.gene062525:CDS | 45.0% |
| AGCGAAAAAGGCGAGGACAT+GGG | - | chr8.1:44642197-44642216 | None:intergenic | 50.0% | |
| ATGCAGAATCGGTCTGGGTT+TGG | - | chr8.1:44642037-44642056 | None:intergenic | 50.0% | |
| ATTGTCCTCGAAAACCTCGC+CGG | + | chr8.1:44642229-44642248 | MS.gene062525:CDS | 50.0% | |
| CTTTCATGAATCTTCCGGCG+AGG | - | chr8.1:44642246-44642265 | None:intergenic | 50.0% | |
| GAAACTGAGTACAGAGCGTG+TGG | + | chr8.1:44642654-44642673 | MS.gene062525:CDS | 50.0% | |
| GAGCGAGAAGAGGTTCTTCT+GGG | - | chr8.1:44642015-44642034 | None:intergenic | 50.0% | |
| GGAACATGCAGAATCGGTCT+GGG | - | chr8.1:44642042-44642061 | None:intergenic | 50.0% | |
| ! | GGAAAGATCGACTTCTTCGG+CGG | - | chr8.1:44642122-44642141 | None:intergenic | 50.0% |
| ! | GTTCCAGTGTTGAAGATCGG+AGG | - | chr8.1:44642143-44642162 | None:intergenic | 50.0% |
| !! | GTTTTCGAGGACAATGCCGT+CGG | - | chr8.1:44642224-44642243 | None:intergenic | 50.0% |
| CACTGGAACAACCTCACCGA+CGG | + | chr8.1:44642154-44642173 | MS.gene062525:CDS | 55.0% | |
| CAGCGAAAAAGGCGAGGACA+TGG | - | chr8.1:44642198-44642217 | None:intergenic | 55.0% | |
| CGAGGACATGGGAAACGAAG+TGG | - | chr8.1:44642186-44642205 | None:intergenic | 55.0% | |
| GGAGCGAGAAGAGGTTCTTC+TGG | - | chr8.1:44642016-44642035 | None:intergenic | 55.0% | |
| GGGAACATGCAGAATCGGTC+TGG | - | chr8.1:44642043-44642062 | None:intergenic | 55.0% | |
| TTCATCCCAACCAACGCGCA+AGG | - | chr8.1:44642736-44642755 | None:intergenic | 55.0% | |
| ! | ATCTTCCGGCGAGGTTTTCG+AGG | - | chr8.1:44642237-44642256 | None:intergenic | 55.0% |
| ! | TCAGAAGCGCGTGCGTTCTA+TGG | + | chr8.1:44642277-44642296 | MS.gene062525:CDS | 55.0% |
| ! | TCTGGGTTTGGAGCGAGAAG+AGG | - | chr8.1:44642025-44642044 | None:intergenic | 55.0% |
| !! | GCCTTTTTCGCTGCATCCGA+CGG | + | chr8.1:44642205-44642224 | MS.gene062525:CDS | 55.0% |
| !! | TGCGCGTTGGTTGGGATGAA+TGG | + | chr8.1:44642736-44642755 | MS.gene062525:CDS | 55.0% |
| AACGAAGTGGCGTTCGCCGT+CGG | - | chr8.1:44642173-44642192 | None:intergenic | 60.0% | |
| CGGATGCAGCGAAAAAGGCG+AGG | - | chr8.1:44642204-44642223 | None:intergenic | 60.0% | |
| GCCGTCGGATGCAGCGAAAA+AGG | - | chr8.1:44642209-44642228 | None:intergenic | 60.0% | |
| GGCGGTCCAGAACGAAGCTT+CGG | - | chr8.1:44642104-44642123 | None:intergenic | 60.0% | |
| !! | ACGCACGCGCTTCTGAGACT+TGG | - | chr8.1:44642273-44642292 | None:intergenic | 60.0% |
| !! | CGCTTCCTTGCGCGTTGGTT+GGG | + | chr8.1:44642728-44642747 | MS.gene062525:CDS | 60.0% |
| CGATGCGCTTCCTTGCGCGT+TGG | + | chr8.1:44642723-44642742 | MS.gene062525:CDS | 65.0% | |
| ! | GCGCTTCCTTGCGCGTTGGT+TGG | + | chr8.1:44642727-44642746 | MS.gene062525:CDS | 65.0% |
| AGTGGCGTTCGCCGTCGGTG+AGG | - | chr8.1:44642168-44642187 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 44642001 | 44642978 | 44642001 | ID=MS.gene062525 |
| chr8.1 | mRNA | 44642001 | 44642978 | 44642001 | ID=MS.gene062525.t1;Parent=MS.gene062525 |
| chr8.1 | exon | 44642001 | 44642978 | 44642001 | ID=MS.gene062525.t1.exon1;Parent=MS.gene062525.t1 |
| chr8.1 | CDS | 44642001 | 44642978 | 44642001 | ID=cds.MS.gene062525.t1;Parent=MS.gene062525.t1 |
| Gene Sequence |
| Protein sequence |
