Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062605.t1 | XP_003619775.1 | 99.1 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 9.70E-54 | 219.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062605.t1 | G7KPB8 | 99.1 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 7.0e-54 | 219.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049703 | MS.gene062605 | -0.804966 | 1.68E-49 | -1.69E-46 |
| MS.gene053109 | MS.gene062605 | 0.818999 | 1.46E-52 | -1.69E-46 |
| MS.gene056432 | MS.gene062605 | 0.823505 | 1.34E-53 | -1.69E-46 |
| MS.gene057057 | MS.gene062605 | 0.806743 | 7.09E-50 | -1.69E-46 |
| MS.gene059719 | MS.gene062605 | 0.810301 | 1.23E-50 | -1.69E-46 |
| MS.gene060465 | MS.gene062605 | 0.804905 | 1.73E-49 | -1.69E-46 |
| MS.gene060702 | MS.gene062605 | 0.835842 | 1.33E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062605.t1 | MTR_6g068850 | 99.091 | 110 | 1 | 0 | 1 | 110 | 1 | 110 | 2.83e-78 | 225 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062605.t1 | AT5G51960 | 60.000 | 110 | 37 | 1 | 1 | 110 | 1 | 103 | 2.31e-42 | 134 |
| MS.gene062605.t1 | AT5G51960 | 60.000 | 110 | 37 | 1 | 1 | 110 | 1 | 103 | 2.31e-42 | 134 |
Find 19 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTATACTTACATGCTTAA+TGG | 0.209140 | 6.4:+47321293 | MS.gene062605:CDS |
| AAGGCGGTCAAGAAACATAT+TGG | 0.364646 | 6.4:+47321161 | MS.gene062605:CDS |
| GGCGATAGATGTTAACTGTT+TGG | 0.367879 | 6.4:-47321130 | None:intergenic |
| TCTTGACCGCCTTGAGAAGA+TGG | 0.432707 | 6.4:-47321151 | None:intergenic |
| CAGACAGATTTCGATTCTTG+TGG | 0.474409 | 6.4:-47321232 | None:intergenic |
| AGGCGGTCAAGAAACATATT+GGG | 0.477390 | 6.4:+47321162 | MS.gene062605:CDS |
| ATAGATGTTAACTGTTTGGA+AGG | 0.518205 | 6.4:-47321126 | None:intergenic |
| CATTATGATTGATAAACCTC+GGG | 0.535278 | 6.4:-47321818 | None:intergenic |
| GGAAAATCTGCTGCAAGTGT+TGG | 0.562393 | 6.4:+47321785 | MS.gene062605:CDS |
| TCATTATGATTGATAAACCT+CGG | 0.563220 | 6.4:-47321819 | None:intergenic |
| CATCTATCGCCATCTTCTCA+AGG | 0.575149 | 6.4:+47321142 | MS.gene062605:CDS |
| AATGAAGTGAAAAGAACACT+TGG | 0.586563 | 6.4:+47321764 | MS.gene062605:CDS |
| AAGAATCGAAATCTGTCTGA+TGG | 0.587712 | 6.4:+47321236 | MS.gene062605:CDS |
| AACAAAGCAATACCTTGTGA+TGG | 0.597418 | 6.4:-47321322 | None:intergenic |
| TAATGGTGTGCACCATCACA+AGG | 0.617495 | 6.4:+47321310 | MS.gene062605:CDS |
| TCGAAATCTGTCTGATGGTG+TGG | 0.617954 | 6.4:+47321241 | MS.gene062605:CDS |
| TCGATTCTTGTGGAACTCCG+AGG | 0.651493 | 6.4:-47321222 | None:intergenic |
| TGTTGGTCTTCAGCTTCCCG+AGG | 0.654165 | 6.4:+47321802 | MS.gene062605:CDS |
| CTATCGCCATCTTCTCAAGG+CGG | 0.739647 | 6.4:+47321145 | MS.gene062605:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAGTGTAAAATCATTACT+AGG | + | chr6.4:47321642-47321661 | MS.gene062605:intron | 20.0% |
| !!! | TTTTTTAGAATTTGTAACCT+CGG | + | chr6.4:47321205-47321224 | MS.gene062605:CDS | 20.0% |
| ! | AAAATTAGCCAAACAAAATG+TGG | - | chr6.4:47321674-47321693 | None:intergenic | 25.0% |
| ! | GATTATACTTACATGCTTAA+TGG | + | chr6.4:47321293-47321312 | MS.gene062605:CDS | 25.0% |
| ! | TGTGTTGCAATATAAAAGTA+AGG | + | chr6.4:47321480-47321499 | MS.gene062605:intron | 25.0% |
| AATACAAGTGACTGTTTGAA+GGG | - | chr6.4:47321361-47321380 | None:intergenic | 30.0% | |
| AATGAAGTGAAAAGAACACT+TGG | + | chr6.4:47321764-47321783 | MS.gene062605:CDS | 30.0% | |
| ATAGATGTTAACTGTTTGGA+AGG | - | chr6.4:47321129-47321148 | None:intergenic | 30.0% | |
| TAATGAGATATCATCAGCTT+TGG | + | chr6.4:47321440-47321459 | MS.gene062605:intron | 30.0% | |
| ! | GGAAAAAACCACATTTTGTT+TGG | + | chr6.4:47321663-47321682 | MS.gene062605:intron | 30.0% |
| AACAAAGCAATACCTTGTGA+TGG | - | chr6.4:47321325-47321344 | None:intergenic | 35.0% | |
| AAGAATCGAAATCTGTCTGA+TGG | + | chr6.4:47321236-47321255 | MS.gene062605:CDS | 35.0% | |
| AGCCTGTACATTATTCTGAT+TGG | + | chr6.4:47321385-47321404 | MS.gene062605:intron | 35.0% | |
| ATCATTGTCATCAATCTTGC+AGG | + | chr6.4:47321703-47321722 | MS.gene062605:intron | 35.0% | |
| CAATACAAGTGACTGTTTGA+AGG | - | chr6.4:47321362-47321381 | None:intergenic | 35.0% | |
| CTCTGCAGATTGTCTTATAA+TGG | + | chr6.4:47321580-47321599 | MS.gene062605:intron | 35.0% | |
| GACCAATCAGAATAATGTAC+AGG | - | chr6.4:47321390-47321409 | None:intergenic | 35.0% | |
| TAAGACAATCTGCAGAGTAT+TGG | - | chr6.4:47321577-47321596 | None:intergenic | 35.0% | |
| !!! | GAGCAAGCTTTATTTTCTGT+TGG | - | chr6.4:47321271-47321290 | None:intergenic | 35.0% |
| AAAAGTAAGGCGTGTAGAAG+TGG | + | chr6.4:47321493-47321512 | MS.gene062605:intron | 40.0% | |
| AAGGCGGTCAAGAAACATAT+TGG | + | chr6.4:47321161-47321180 | MS.gene062605:CDS | 40.0% | |
| AGGCGGTCAAGAAACATATT+GGG | + | chr6.4:47321162-47321181 | MS.gene062605:CDS | 40.0% | |
| CAGACAGATTTCGATTCTTG+TGG | - | chr6.4:47321235-47321254 | None:intergenic | 40.0% | |
| GGCGATAGATGTTAACTGTT+TGG | - | chr6.4:47321133-47321152 | None:intergenic | 40.0% | |
| CATCTATCGCCATCTTCTCA+AGG | + | chr6.4:47321142-47321161 | MS.gene062605:CDS | 45.0% | |
| GCAATTCAGCATAGCCAAGT+TGG | + | chr6.4:47321544-47321563 | MS.gene062605:intron | 45.0% | |
| GGAAAATCTGCTGCAAGTGT+TGG | + | chr6.4:47321785-47321804 | MS.gene062605:CDS | 45.0% | |
| TAATGGTGTGCACCATCACA+AGG | + | chr6.4:47321310-47321329 | MS.gene062605:CDS | 45.0% | |
| ! | TCGAAATCTGTCTGATGGTG+TGG | + | chr6.4:47321241-47321260 | MS.gene062605:CDS | 45.0% |
| !! | CAATCTGCAGAGTATTGGCT+TGG | - | chr6.4:47321572-47321591 | None:intergenic | 45.0% |
| !! | GTATTGGCTTGGATCCAACT+TGG | - | chr6.4:47321561-47321580 | None:intergenic | 45.0% |
| CTATCGCCATCTTCTCAAGG+CGG | + | chr6.4:47321145-47321164 | MS.gene062605:CDS | 50.0% | |
| TCGATTCTTGTGGAACTCCG+AGG | - | chr6.4:47321225-47321244 | None:intergenic | 50.0% | |
| TCTTGACCGCCTTGAGAAGA+TGG | - | chr6.4:47321154-47321173 | None:intergenic | 50.0% | |
| !! | TGTTGGTCTTCAGCTTCCCG+AGG | + | chr6.4:47321802-47321821 | MS.gene062605:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 47321113 | 47321838 | 47321113 | ID=MS.gene062605 |
| chr6.4 | mRNA | 47321113 | 47321838 | 47321113 | ID=MS.gene062605.t1;Parent=MS.gene062605 |
| chr6.4 | exon | 47321113 | 47321331 | 47321113 | ID=MS.gene062605.t1.exon1;Parent=MS.gene062605.t1 |
| chr6.4 | CDS | 47321113 | 47321331 | 47321113 | ID=cds.MS.gene062605.t1;Parent=MS.gene062605.t1 |
| chr6.4 | exon | 47321725 | 47321838 | 47321725 | ID=MS.gene062605.t1.exon2;Parent=MS.gene062605.t1 |
| chr6.4 | CDS | 47321725 | 47321838 | 47321725 | ID=cds.MS.gene062605.t1;Parent=MS.gene062605.t1 |
| Gene Sequence |
| Protein sequence |
