Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049703.t1 | XP_024638328.1 | 76.9 | 78 | 13 | 1 | 54 | 126 | 242 | 319 | 1.10E-19 | 106.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049703.t1 | A0A396GQG1 | 74.4 | 78 | 15 | 1 | 54 | 126 | 192 | 269 | 3.5e-18 | 100.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene049703 | -0.805981 | 1.03E-49 | -1.69E-46 |
| MS.gene04917 | MS.gene049703 | -0.800458 | 1.43E-48 | -1.69E-46 |
| MS.gene049703 | MS.gene051245 | -0.805315 | 1.42E-49 | -1.69E-46 |
| MS.gene049703 | MS.gene057057 | -0.807366 | 5.24E-50 | -1.69E-46 |
| MS.gene049703 | MS.gene062605 | -0.804966 | 1.68E-49 | -1.69E-46 |
| MS.gene049703 | MS.gene063544 | -0.802468 | 5.53E-49 | -1.69E-46 |
| MS.gene049703 | MS.gene065136 | 0.822039 | 2.93E-53 | -1.69E-46 |
| MS.gene049703 | MS.gene06865 | -0.804371 | 2.23E-49 | -1.69E-46 |
| MS.gene049703 | MS.gene070222 | -0.807996 | 3.85E-50 | -1.69E-46 |
| MS.gene049703 | MS.gene072386 | -0.814495 | 1.50E-51 | -1.69E-46 |
| MS.gene049703 | MS.gene072907 | -0.804376 | 2.23E-49 | -1.69E-46 |
| MS.gene049703 | MS.gene08889 | -0.809909 | 1.50E-50 | -1.69E-46 |
| MS.gene049703 | MS.gene09400 | -0.810862 | 9.34E-51 | -1.69E-46 |
| MS.gene049703 | MS.gene22314 | -0.800532 | 1.38E-48 | -1.69E-46 |
| MS.gene049703 | MS.gene22499 | -0.802562 | 5.29E-49 | -1.69E-46 |
| MS.gene049703 | MS.gene22545 | -0.807306 | 5.39E-50 | -1.69E-46 |
| MS.gene049703 | MS.gene26426 | -0.8005 | 1.40E-48 | -1.69E-46 |
| MS.gene049703 | MS.gene30792 | 0.819948 | 8.88E-53 | -1.69E-46 |
| MS.gene049703 | MS.gene36146 | -0.81243 | 4.26E-51 | -1.69E-46 |
| MS.gene049703 | MS.gene36147 | -0.817171 | 3.79E-52 | -1.69E-46 |
| MS.gene049703 | MS.gene36627 | -0.807017 | 6.21E-50 | -1.69E-46 |
| MS.gene049703 | MS.gene39222 | -0.805977 | 1.03E-49 | -1.69E-46 |
| MS.gene049703 | MS.gene39317 | -0.804875 | 1.75E-49 | -1.69E-46 |
| MS.gene049703 | MS.gene44135 | 0.827973 | 1.16E-54 | -1.69E-46 |
| MS.gene049703 | MS.gene44260 | -0.800873 | 1.17E-48 | -1.69E-46 |
| MS.gene049703 | MS.gene46281 | 0.825702 | 4.06E-54 | -1.69E-46 |
| MS.gene049703 | MS.gene52153 | -0.821419 | 4.08E-53 | -1.69E-46 |
| MS.gene049703 | MS.gene63281 | -0.8039 | 2.80E-49 | -1.69E-46 |
| MS.gene049703 | MS.gene63902 | -0.804187 | 2.44E-49 | -1.69E-46 |
| MS.gene049703 | MS.gene65412 | -0.825857 | 3.73E-54 | -1.69E-46 |
| MS.gene049703 | MS.gene68171 | -0.82132 | 4.30E-53 | -1.69E-46 |
| MS.gene049703 | MS.gene68810 | -0.802596 | 5.20E-49 | -1.69E-46 |
| MS.gene049703 | MS.gene68840 | -0.831869 | 1.31E-55 | -1.69E-46 |
| MS.gene049703 | MS.gene70378 | -0.812765 | 3.60E-51 | -1.69E-46 |
| MS.gene049703 | MS.gene76046 | -0.800917 | 1.15E-48 | -1.69E-46 |
| MS.gene049703 | MS.gene76478 | -0.817172 | 3.79E-52 | -1.69E-46 |
| MS.gene049703 | MS.gene87399 | -0.804794 | 1.82E-49 | -1.69E-46 |
| MS.gene049703 | MS.gene87972 | -0.810335 | 1.21E-50 | -1.69E-46 |
| MS.gene049703 | MS.gene94949 | -0.849427 | 3.26E-60 | -1.69E-46 |
| MS.gene049703 | MS.gene99313 | -0.808393 | 3.17E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049703.t1 | MTR_8g085310 | 45.946 | 74 | 34 | 2 | 56 | 126 | 613 | 683 | 3.61e-11 | 59.7 |
| MS.gene049703.t1 | MTR_4g051480 | 44.737 | 76 | 32 | 2 | 54 | 126 | 751 | 819 | 5.68e-11 | 59.3 |
| MS.gene049703.t1 | MTR_4g092300 | 44.737 | 76 | 32 | 2 | 54 | 126 | 733 | 801 | 5.83e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCATGGGCTAAAGTTTCTT+TGG | 0.327848 | 3.3:-58296607 | MS.gene049703:CDS |
| ATGTGATGTAGACTCCTTCT+TGG | 0.355967 | 3.3:+58296650 | None:intergenic |
| GGAGCAACCGAGGAGGAGGC+AGG | 0.397527 | 3.3:+58297640 | None:intergenic |
| TATGGAAAAGTTTATGAATC+CGG | 0.398707 | 3.3:-58296477 | MS.gene049703:CDS |
| TCGGTTGCTCCTGCTTCTAC+TGG | 0.415271 | 3.3:-58297628 | MS.gene049703:CDS |
| TCATGAACTAGAAGACATTT+CGG | 0.419596 | 3.3:-58296508 | MS.gene049703:CDS |
| TTTCTTTGGCAAGAGCTGAT+AGG | 0.436030 | 3.3:-58296593 | MS.gene049703:CDS |
| GATACCTGTGCAGCAGTGTC+AGG | 0.474964 | 3.3:+58297601 | None:intergenic |
| AGCAGGAGCAACCGAGGAGG+AGG | 0.476005 | 3.3:+58297636 | None:intergenic |
| AACCGAGGAGGAGGCAGGCG+CGG | 0.482066 | 3.3:+58297645 | None:intergenic |
| CACACGCAGTGAAAAGCAAC+AGG | 0.495677 | 3.3:-58296685 | MS.gene049703:intron |
| GAATGAGGCACTTGCAGCAT+GGG | 0.501641 | 3.3:-58296622 | MS.gene049703:CDS |
| GGCCGCGCCTGCCTCCTCCT+CGG | 0.506445 | 3.3:-58297647 | MS.gene049703:CDS |
| AAAAGTTTATGAATCCGGAT+TGG | 0.515747 | 3.3:-58296472 | MS.gene049703:CDS |
| GACATTTCGGATGATGCCTA+TGG | 0.521967 | 3.3:-58296495 | MS.gene049703:CDS |
| GGCGCAACAAATGAGGAGGC+AGG | 0.525124 | 3.3:+58297685 | None:intergenic |
| AGTTTATGAATCCGGATTGG+AGG | 0.528903 | 3.3:-58296469 | MS.gene049703:CDS |
| TGCGCCTTCAACCTCTTCAT+CGG | 0.532285 | 3.3:-58297668 | MS.gene049703:CDS |
| GTTTATGAATCCGGATTGGA+GGG | 0.534860 | 3.3:-58296468 | MS.gene049703:CDS |
| TGTACCTGACACTGCTGCAC+AGG | 0.537919 | 3.3:-58297605 | MS.gene049703:intron |
| ATATACACTTCCCTCCAATC+CGG | 0.538012 | 3.3:+58296458 | None:intergenic |
| GGCAGGCGCGGCCGATGAAG+AGG | 0.539594 | 3.3:+58297657 | None:intergenic |
| TCAGGTACACCAGTAGAAGC+AGG | 0.562120 | 3.3:+58297619 | None:intergenic |
| GGTTGAAGGCGCAACAAATG+AGG | 0.573929 | 3.3:+58297678 | None:intergenic |
| GCGGCCGATGAAGAGGTTGA+AGG | 0.578826 | 3.3:+58297664 | None:intergenic |
| AGTAGAAGCAGGAGCAACCG+AGG | 0.584298 | 3.3:+58297630 | None:intergenic |
| TACATCACATCATATGAATG+AGG | 0.591772 | 3.3:-58296637 | MS.gene049703:CDS |
| TGAATGAGGCACTTGCAGCA+TGG | 0.593350 | 3.3:-58296623 | MS.gene049703:CDS |
| ATTGCTAACTGCATAACTCA+TGG | 0.595136 | 3.3:+58297746 | None:intergenic |
| TTGTTCTCTTGCAAAGTCTG+TGG | 0.606108 | 3.3:-58296538 | MS.gene049703:CDS |
| GAGGCAGGAGCAGCGAAACG+TGG | 0.608035 | 3.3:+58297700 | None:intergenic |
| AAGAGCTGATAGGAGTGCAG+AGG | 0.619518 | 3.3:-58296583 | MS.gene049703:CDS |
| GGTTCGTGTGAGATCCAAGA+AGG | 0.624211 | 3.3:-58296664 | MS.gene049703:CDS |
| AGAAGCAGGAGCAACCGAGG+AGG | 0.653647 | 3.3:+58297633 | None:intergenic |
| ACGCTTCCTCTCATTAGACA+TGG | 0.653912 | 3.3:+58296433 | None:intergenic |
| TGAAGGCGCAACAAATGAGG+AGG | 0.654904 | 3.3:+58297681 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAAAACAAACAAAAATG+AGG | + | chr3.3:58296718-58296737 | None:intergenic | 15.0% |
| !! | CAAGTTATGTTATATAAAAA+TGG | + | chr3.3:58297403-58297422 | None:intergenic | 15.0% |
| !!! | GTTGTAAATTTTTTATTTGT+TGG | - | chr3.3:58297132-58297151 | MS.gene049703:intron | 15.0% |
| !! | AGTACAATAAAATCTAATAC+AGG | + | chr3.3:58297009-58297028 | None:intergenic | 20.0% |
| !!! | TAGATTTTATTGTACTTCTA+TGG | - | chr3.3:58297013-58297032 | MS.gene049703:intron | 20.0% |
| ! | ACAGAACAGATTGAATTAAT+GGG | + | chr3.3:58297111-58297130 | None:intergenic | 25.0% |
| ! | AGAAAAACGCATAAAAAGTA+TGG | + | chr3.3:58296766-58296785 | None:intergenic | 25.0% |
| ! | ATGTATAACATAACAGAACA+AGG | + | chr3.3:58297203-58297222 | None:intergenic | 25.0% |
| ! | TATGGAAAAGTTTATGAATC+CGG | - | chr3.3:58297713-58297732 | MS.gene049703:CDS | 25.0% |
| ! | TGGTTTCTAAATGTGTATTA+TGG | - | chr3.3:58297152-58297171 | MS.gene049703:intron | 25.0% |
| AAAAGTTTATGAATCCGGAT+TGG | - | chr3.3:58297718-58297737 | MS.gene049703:CDS | 30.0% | |
| GACAGAACAGATTGAATTAA+TGG | + | chr3.3:58297112-58297131 | None:intergenic | 30.0% | |
| GGTTAGGCTTTACATATATA+GGG | - | chr3.3:58296931-58296950 | MS.gene049703:intron | 30.0% | |
| GTTAGGCTTTACATATATAG+GGG | - | chr3.3:58296932-58296951 | MS.gene049703:intron | 30.0% | |
| TACATCACATCATATGAATG+AGG | - | chr3.3:58297553-58297572 | MS.gene049703:intron | 30.0% | |
| TAGATGACTGAGATTATAGA+TGG | + | chr3.3:58296630-58296649 | None:intergenic | 30.0% | |
| TATGTGTATCTATCCAACAA+GGG | + | chr3.3:58297267-58297286 | None:intergenic | 30.0% | |
| TCATGAACTAGAAGACATTT+CGG | - | chr3.3:58297682-58297701 | MS.gene049703:CDS | 30.0% | |
| TGATGACAATTAGTGAGTTA+TGG | - | chr3.3:58296891-58296910 | MS.gene049703:intron | 30.0% | |
| TGGTTAGGCTTTACATATAT+AGG | - | chr3.3:58296930-58296949 | MS.gene049703:intron | 30.0% | |
| ! | AATCAATTTGAGGCAATAAG+AGG | + | chr3.3:58297237-58297256 | None:intergenic | 30.0% |
| ! | GGATTCATAAACTTTTCCAT+AGG | + | chr3.3:58297714-58297733 | None:intergenic | 30.0% |
| !! | AGTTAGTTGTTTCTTTGGTT+AGG | - | chr3.3:58296915-58296934 | MS.gene049703:intron | 30.0% |
| ATGTGTATCTATCCAACAAG+GGG | + | chr3.3:58297266-58297285 | None:intergenic | 35.0% | |
| ATTATAGATGGCTGAGATGA+AGG | + | chr3.3:58296618-58296637 | None:intergenic | 35.0% | |
| CAAAAATTTCAGCAGCAGTT+TGG | + | chr3.3:58296743-58296762 | None:intergenic | 35.0% | |
| GTATGTGTATCTATCCAACA+AGG | + | chr3.3:58297268-58297287 | None:intergenic | 35.0% | |
| TAACAGAACAAGGTGTAGTT+TGG | + | chr3.3:58297193-58297212 | None:intergenic | 35.0% | |
| ! | ACTTTTTATGTGCAGTAGGA+GGG | - | chr3.3:58296789-58296808 | MS.gene049703:intron | 35.0% |
| ! | CACTTTTGTGCAATATGATC+AGG | + | chr3.3:58297367-58297386 | None:intergenic | 35.0% |
| !! | ATGGCAGTTAGTTGTTTCTT+TGG | - | chr3.3:58296910-58296929 | MS.gene049703:intron | 35.0% |
| !! | TTGCACTTTTTATGTGCAGT+AGG | - | chr3.3:58296785-58296804 | MS.gene049703:intron | 35.0% |
| ACATATATAGGGGAGTCGTA+AGG | - | chr3.3:58296942-58296961 | MS.gene049703:intron | 40.0% | |
| ACTATCATCCTTGTCTGGAA+TGG | - | chr3.3:58297084-58297103 | MS.gene049703:intron | 40.0% | |
| ATATACACTTCCCTCCAATC+CGG | + | chr3.3:58297735-58297754 | None:intergenic | 40.0% | |
| ATGTGATGTAGACTCCTTCT+TGG | + | chr3.3:58297543-58297562 | None:intergenic | 40.0% | |
| GGGGAATGAGAATCAATTTG+AGG | + | chr3.3:58297247-58297266 | None:intergenic | 40.0% | |
| TGCCATCAGTTAGTTACACA+GGG | + | chr3.3:58296844-58296863 | None:intergenic | 40.0% | |
| TTGACACTATCATCCTTGTC+TGG | - | chr3.3:58297079-58297098 | MS.gene049703:intron | 40.0% | |
| TTGATTCTCATTCCCCTTGT+TGG | - | chr3.3:58297251-58297270 | MS.gene049703:intron | 40.0% | |
| TTGTTCTCTTGCAAAGTCTG+TGG | - | chr3.3:58297652-58297671 | MS.gene049703:CDS | 40.0% | |
| TTTCTTTGGCAAGAGCTGAT+AGG | - | chr3.3:58297597-58297616 | MS.gene049703:intron | 40.0% | |
| ! | AGCATGGGCTAAAGTTTCTT+TGG | - | chr3.3:58297583-58297602 | MS.gene049703:intron | 40.0% |
| ! | AGTTTATGAATCCGGATTGG+AGG | - | chr3.3:58297721-58297740 | MS.gene049703:CDS | 40.0% |
| ! | GTTTATGAATCCGGATTGGA+GGG | - | chr3.3:58297722-58297741 | MS.gene049703:CDS | 40.0% |
| !! | CACTTTTTATGTGCAGTAGG+AGG | - | chr3.3:58296788-58296807 | MS.gene049703:intron | 40.0% |
| AATGAGGCCAACCAAAAGAG+TGG | + | chr3.3:58296702-58296721 | None:intergenic | 45.0% | |
| CTGCCATCAGTTAGTTACAC+AGG | + | chr3.3:58296845-58296864 | None:intergenic | 45.0% | |
| GACATTTCGGATGATGCCTA+TGG | - | chr3.3:58297695-58297714 | MS.gene049703:CDS | 45.0% | |
| GACCCTGTGTAACTAACTGA+TGG | - | chr3.3:58296839-58296858 | MS.gene049703:intron | 45.0% | |
| TAATGGGACCATTCCAGACA+AGG | + | chr3.3:58297095-58297114 | None:intergenic | 45.0% | |
| TAGATGGCTGAGATGAAGGT+AGG | + | chr3.3:58296614-58296633 | None:intergenic | 45.0% | |
| TTATGTGCAGTAGGAGGGAT+AGG | - | chr3.3:58296794-58296813 | MS.gene049703:intron | 45.0% | |
| ! | ATTGCGACCACTCTTTTGGT+TGG | - | chr3.3:58296692-58296711 | MS.gene049703:intron | 45.0% |
| ! | TTGCATTGCGACCACTCTTT+TGG | - | chr3.3:58296688-58296707 | MS.gene049703:intron | 45.0% |
| CACACGCAGTGAAAAGCAAC+AGG | - | chr3.3:58297505-58297524 | MS.gene049703:intron | 50.0% | |
| GGTTCGTGTGAGATCCAAGA+AGG | - | chr3.3:58297526-58297545 | MS.gene049703:intron | 50.0% | |
| TCAGGTACACCAGTAGAAGC+AGG | + | chr3.3:58296574-58296593 | None:intergenic | 50.0% | |
| TGCGCCTTCAACCTCTTCAT+CGG | - | chr3.3:58296522-58296541 | MS.gene049703:CDS | 50.0% | |
| ! | AAGAGCTGATAGGAGTGCAG+AGG | - | chr3.3:58297607-58297626 | MS.gene049703:CDS | 50.0% |
| ! | GAATGAGGCACTTGCAGCAT+GGG | - | chr3.3:58297568-58297587 | MS.gene049703:intron | 50.0% |
| ! | GGTTGAAGGCGCAACAAATG+AGG | + | chr3.3:58296515-58296534 | None:intergenic | 50.0% |
| ! | TGAAGGCGCAACAAATGAGG+AGG | + | chr3.3:58296512-58296531 | None:intergenic | 50.0% |
| ! | TGAATGAGGCACTTGCAGCA+TGG | - | chr3.3:58297567-58297586 | MS.gene049703:intron | 50.0% |
| AGTAGAAGCAGGAGCAACCG+AGG | + | chr3.3:58296563-58296582 | None:intergenic | 55.0% | |
| GATACCTGTGCAGCAGTGTC+AGG | + | chr3.3:58296592-58296611 | None:intergenic | 55.0% | |
| GTGCAGTAGGAGGGATAGGA+GGG | - | chr3.3:58296798-58296817 | MS.gene049703:intron | 55.0% | |
| TCGGTTGCTCCTGCTTCTAC+TGG | - | chr3.3:58296562-58296581 | MS.gene049703:CDS | 55.0% | |
| TGTACCTGACACTGCTGCAC+AGG | - | chr3.3:58296585-58296604 | MS.gene049703:CDS | 55.0% | |
| TGTGCAGTAGGAGGGATAGG+AGG | - | chr3.3:58296797-58296816 | MS.gene049703:intron | 55.0% | |
| AGAAGCAGGAGCAACCGAGG+AGG | + | chr3.3:58296560-58296579 | None:intergenic | 60.0% | |
| GCGGCCGATGAAGAGGTTGA+AGG | + | chr3.3:58296529-58296548 | None:intergenic | 60.0% | |
| GGCGCAACAAATGAGGAGGC+AGG | + | chr3.3:58296508-58296527 | None:intergenic | 60.0% | |
| AGCAGGAGCAACCGAGGAGG+AGG | + | chr3.3:58296557-58296576 | None:intergenic | 65.0% | |
| GAGGCAGGAGCAGCGAAACG+TGG | + | chr3.3:58296493-58296512 | None:intergenic | 65.0% | |
| AACCGAGGAGGAGGCAGGCG+CGG | + | chr3.3:58296548-58296567 | None:intergenic | 70.0% | |
| GGAGCAACCGAGGAGGAGGC+AGG | + | chr3.3:58296553-58296572 | None:intergenic | 70.0% | |
| GGCAGGCGCGGCCGATGAAG+AGG | + | chr3.3:58296536-58296555 | None:intergenic | 75.0% | |
| ! | GGCCGCGCCTGCCTCCTCCT+CGG | - | chr3.3:58296543-58296562 | MS.gene049703:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 58296446 | 58297766 | 58296446 | ID=MS.gene049703 |
| chr3.3 | mRNA | 58296446 | 58297766 | 58296446 | ID=MS.gene049703.t1;Parent=MS.gene049703 |
| chr3.3 | exon | 58297606 | 58297766 | 58297606 | ID=MS.gene049703.t1.exon1;Parent=MS.gene049703.t1 |
| chr3.3 | CDS | 58297606 | 58297766 | 58297606 | ID=cds.MS.gene049703.t1;Parent=MS.gene049703.t1 |
| chr3.3 | exon | 58296446 | 58296698 | 58296446 | ID=MS.gene049703.t1.exon2;Parent=MS.gene049703.t1 |
| chr3.3 | CDS | 58296446 | 58296698 | 58296446 | ID=cds.MS.gene049703.t1;Parent=MS.gene049703.t1 |
| Gene Sequence |
| Protein sequence |
