Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063627.t1 | RHN60700.1 | 99.4 | 178 | 1 | 0 | 1 | 178 | 4 | 181 | 4.30E-51 | 211.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063627.t1 | Q9H6L4 | 64.3 | 56 | 20 | 0 | 19 | 74 | 13 | 68 | 4.0e-13 | 76.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063627.t1 | A0A396I501 | 99.4 | 178 | 1 | 0 | 1 | 178 | 4 | 181 | 3.1e-51 | 211.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene00474 | MS.gene063627 | PPI |
| MS.gene063627 | MS.gene044085 | PPI |
| MS.gene063627 | MS.gene00478 | PPI |
| MS.gene063627 | MS.gene24450 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063627.t1 | MTR_5g077040 | 98.315 | 178 | 3 | 0 | 1 | 178 | 1 | 178 | 2.97e-128 | 357 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063627.t1 | AT5G37290 | 72.067 | 179 | 48 | 1 | 1 | 177 | 1 | 179 | 4.60e-99 | 283 |
| MS.gene063627.t1 | AT5G37290 | 72.067 | 179 | 48 | 1 | 1 | 177 | 1 | 179 | 4.60e-99 | 283 |
| MS.gene063627.t1 | AT5G37290 | 71.311 | 122 | 35 | 0 | 1 | 122 | 1 | 122 | 1.30e-65 | 197 |
Find 42 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTGTTGCCTTTGGTTAT+TGG | 0.174361 | 5.2:-65797748 | None:intergenic |
| AATGAAAAGATTATAGAATT+TGG | 0.211644 | 5.2:+65798283 | MS.gene063627:CDS |
| AGAAATTAAAGAGAAGATTT+TGG | 0.229064 | 5.2:+65798058 | MS.gene063627:intron |
| ATAATTGCAGCTAAATGTTT+TGG | 0.267268 | 5.2:+65798231 | MS.gene063627:intron |
| ATCCTTACAGTGTTCCTAAC+TGG | 0.299126 | 5.2:-65798496 | None:intergenic |
| CGGTCCGCTCTTGTTGCCTT+TGG | 0.333981 | 5.2:-65797755 | None:intergenic |
| GTTAAAATTGTAAGGATCAT+AGG | 0.342362 | 5.2:-65798099 | None:intergenic |
| TAGTGTGAGCTTCAGTAATC+TGG | 0.391697 | 5.2:+65798872 | MS.gene063627:CDS |
| CAAGAGCGGACCGGAAGATA+TGG | 0.397319 | 5.2:+65797765 | MS.gene063627:CDS |
| CACCTGAAGGTATTGCAATC+TGG | 0.406154 | 5.2:-65797793 | None:intergenic |
| AATGTTTATCAAGCCCAGTT+AGG | 0.407380 | 5.2:+65798482 | MS.gene063627:CDS |
| CGCAAGAAGTTAAAATTGTA+AGG | 0.419936 | 5.2:-65798107 | None:intergenic |
| CCTTTGGTTATTGGTGAACA+TGG | 0.442442 | 5.2:-65797739 | None:intergenic |
| TTGATGACATCAATAACTTC+AGG | 0.448267 | 5.2:-65798825 | None:intergenic |
| TTCCAGATTGCAATACCTTC+AGG | 0.449232 | 5.2:+65797791 | MS.gene063627:CDS |
| GATAAACATTGAATGATAAG+AGG | 0.452418 | 5.2:-65798469 | None:intergenic |
| ATTATAGAATTTGGTGTTGG+AGG | 0.454107 | 5.2:+65798292 | MS.gene063627:CDS |
| GCTACAATTGTAGCGTTGGC+TGG | 0.465004 | 5.2:-65798430 | None:intergenic |
| TCCTTACAGTGTTCCTAACT+GGG | 0.468459 | 5.2:-65798495 | None:intergenic |
| AAGATTATAGAATTTGGTGT+TGG | 0.481352 | 5.2:+65798289 | MS.gene063627:CDS |
| TTCTTGACAAACATCTATCA+AGG | 0.487090 | 5.2:+65798904 | MS.gene063627:CDS |
| TTATAGAATTTGGTGTTGGA+GGG | 0.493970 | 5.2:+65798293 | MS.gene063627:CDS |
| ACAATTGTAGCTGGAGTTGG+TGG | 0.514415 | 5.2:+65798442 | MS.gene063627:CDS |
| TCTGGAAGTACCATATCTTC+CGG | 0.518139 | 5.2:-65797775 | None:intergenic |
| GCCAACGCTACAATTGTAGC+TGG | 0.526233 | 5.2:+65798433 | MS.gene063627:CDS |
| TCCAGCTACAATTGTAGCGT+TGG | 0.532247 | 5.2:-65798434 | None:intergenic |
| GTAAGGATCATAGGCAAAGT+TGG | 0.532617 | 5.2:-65798090 | None:intergenic |
| ATCAGGTAAATTATGCACTC+GGG | 0.532999 | 5.2:+65798754 | MS.gene063627:intron |
| CAAAGGCAACAAGAGCGGAC+CGG | 0.544877 | 5.2:+65797756 | MS.gene063627:CDS |
| GATCAGGTAAATTATGCACT+CGG | 0.549275 | 5.2:+65798753 | MS.gene063627:intron |
| GCTACAATTGTAGCTGGAGT+TGG | 0.558809 | 5.2:+65798439 | MS.gene063627:CDS |
| TAATGAATCTAACAAGGAAG+AGG | 0.565289 | 5.2:+65798794 | MS.gene063627:CDS |
| TGGGTTCAGTTAAGCAGTCA+AGG | 0.565826 | 5.2:-65798261 | None:intergenic |
| GGGATCTGCAATTGTTGTGT+TGG | 0.566984 | 5.2:+65798313 | MS.gene063627:CDS |
| TTTCAGAATTCATCAGAGGA+TGG | 0.593173 | 5.2:+65797918 | MS.gene063627:CDS |
| CAATTGTAGCTGGAGTTGGT+GGG | 0.606878 | 5.2:+65798443 | MS.gene063627:CDS |
| CATCTGTAATGAATCTAACA+AGG | 0.607086 | 5.2:+65798788 | MS.gene063627:CDS |
| AAGTTATTGATGTCATCAAG+AGG | 0.636725 | 5.2:+65798829 | MS.gene063627:CDS |
| TCAGTTTCAGAATTCATCAG+AGG | 0.641335 | 5.2:+65797914 | MS.gene063627:CDS |
| CCATGTTCACCAATAACCAA+AGG | 0.659452 | 5.2:+65797739 | None:intergenic |
| ATAACCAAAGGCAACAAGAG+CGG | 0.667897 | 5.2:+65797751 | MS.gene063627:CDS |
| TCAGGTAAATTATGCACTCG+GGG | 0.677160 | 5.2:+65798755 | MS.gene063627:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAAGATTATAGAATT+TGG | + | chr5.2:65798283-65798302 | MS.gene063627:CDS | 15.0% |
| !!! | AAATTCTATAATCTTTTCAT+TGG | - | chr5.2:65798284-65798303 | None:intergenic | 15.0% |
| !!! | AATTCTATAATCTTTTCATT+GGG | - | chr5.2:65798283-65798302 | None:intergenic | 15.0% |
| !!! | AGTTGTTTATCAAATTTTTA+GGG | - | chr5.2:65798720-65798739 | None:intergenic | 15.0% |
| !!! | TAGTTGTTTATCAAATTTTT+AGG | - | chr5.2:65798721-65798740 | None:intergenic | 15.0% |
| !! | AAACAAACGTTATTCATATA+AGG | - | chr5.2:65797880-65797899 | None:intergenic | 20.0% |
| !!! | AGAAATTAAAGAGAAGATTT+TGG | + | chr5.2:65798058-65798077 | MS.gene063627:intron | 20.0% |
| !!! | GTTGTTTATCAAATTTTTAG+GGG | - | chr5.2:65798719-65798738 | None:intergenic | 20.0% |
| ! | ACTAATGTATTTATCTGATC+AGG | + | chr5.2:65798737-65798756 | MS.gene063627:intron | 25.0% |
| ! | ACTGATGCAAATTTAGTATA+AGG | - | chr5.2:65798596-65798615 | None:intergenic | 25.0% |
| ! | ATATGAATAACGTTTGTTTC+AGG | + | chr5.2:65797881-65797900 | MS.gene063627:intron | 25.0% |
| ! | CTGATGCAAATTTAGTATAA+GGG | - | chr5.2:65798595-65798614 | None:intergenic | 25.0% |
| ! | GATAAACATTGAATGATAAG+AGG | - | chr5.2:65798472-65798491 | None:intergenic | 25.0% |
| ! | GTTAAAATTGTAAGGATCAT+AGG | - | chr5.2:65798102-65798121 | None:intergenic | 25.0% |
| ! | TTATTGCAAAGACAATTAGT+GGG | - | chr5.2:65798144-65798163 | None:intergenic | 25.0% |
| !! | ATAATTGCAGCTAAATGTTT+TGG | + | chr5.2:65798231-65798250 | MS.gene063627:intron | 25.0% |
| !!! | AAGATTATAGAATTTGGTGT+TGG | + | chr5.2:65798289-65798308 | MS.gene063627:CDS | 25.0% |
| AAGTTATTGATGTCATCAAG+AGG | + | chr5.2:65798829-65798848 | MS.gene063627:CDS | 30.0% | |
| AGATAGAAATCACAATACTG+TGG | - | chr5.2:65798360-65798379 | None:intergenic | 30.0% | |
| ATAAAGAATCCTACCAACAA+AGG | - | chr5.2:65797993-65798012 | None:intergenic | 30.0% | |
| ATAACGTTTGTTTCAGGAAT+TGG | + | chr5.2:65797887-65797906 | MS.gene063627:intron | 30.0% | |
| CATCTGTAATGAATCTAACA+AGG | + | chr5.2:65798788-65798807 | MS.gene063627:CDS | 30.0% | |
| CGCAAGAAGTTAAAATTGTA+AGG | - | chr5.2:65798110-65798129 | None:intergenic | 30.0% | |
| CTTATTGCAAAGACAATTAG+TGG | - | chr5.2:65798145-65798164 | None:intergenic | 30.0% | |
| TAATGAATCTAACAAGGAAG+AGG | + | chr5.2:65798794-65798813 | MS.gene063627:CDS | 30.0% | |
| TTCTTGACAAACATCTATCA+AGG | + | chr5.2:65798904-65798923 | MS.gene063627:CDS | 30.0% | |
| ! | TTGATGACATCAATAACTTC+AGG | - | chr5.2:65798828-65798847 | None:intergenic | 30.0% |
| ! | TTTACCATCTCCTTATTTGT+TGG | + | chr5.2:65798014-65798033 | MS.gene063627:intron | 30.0% |
| !! | AATTTTTAGGGGATCTTTTG+TGG | - | chr5.2:65798708-65798727 | None:intergenic | 30.0% |
| !! | AGAGAAGATTTTGGCAAATT+TGG | + | chr5.2:65798067-65798086 | MS.gene063627:CDS | 30.0% |
| !! | ATTATAGAATTTGGTGTTGG+AGG | + | chr5.2:65798292-65798311 | MS.gene063627:CDS | 30.0% |
| !! | TTATAGAATTTGGTGTTGGA+GGG | + | chr5.2:65798293-65798312 | MS.gene063627:CDS | 30.0% |
| !!! | GTTTGCTTGTTGATTTTGTA+AGG | + | chr5.2:65798523-65798542 | MS.gene063627:intron | 30.0% |
| AACACCAACAAATAAGGAGA+TGG | - | chr5.2:65798021-65798040 | None:intergenic | 35.0% | |
| AATCTTTCAAGCACCTTTGT+TGG | + | chr5.2:65797977-65797996 | MS.gene063627:intron | 35.0% | |
| AATGTTTATCAAGCCCAGTT+AGG | + | chr5.2:65798482-65798501 | MS.gene063627:CDS | 35.0% | |
| ACGAGAAACACCAACAAATA+AGG | - | chr5.2:65798027-65798046 | None:intergenic | 35.0% | |
| AGAAATCACAATACTGTGGA+AGG | - | chr5.2:65798356-65798375 | None:intergenic | 35.0% | |
| ATCAGGTAAATTATGCACTC+GGG | + | chr5.2:65798754-65798773 | MS.gene063627:intron | 35.0% | |
| GAAGAAGAAAAAACACCTGA+AGG | - | chr5.2:65797809-65797828 | None:intergenic | 35.0% | |
| GATCAGGTAAATTATGCACT+CGG | + | chr5.2:65798753-65798772 | MS.gene063627:intron | 35.0% | |
| TCAGTTTCAGAATTCATCAG+AGG | + | chr5.2:65797914-65797933 | MS.gene063627:CDS | 35.0% | |
| TTTCAGAATTCATCAGAGGA+TGG | + | chr5.2:65797918-65797937 | MS.gene063627:CDS | 35.0% | |
| !! | ACAAGCGATTTTGAGAAATC+AGG | - | chr5.2:65798623-65798642 | None:intergenic | 35.0% |
| ATAACCAAAGGCAACAAGAG+CGG | + | chr5.2:65797751-65797770 | MS.gene063627:CDS | 40.0% | |
| ATCCTTACAGTGTTCCTAAC+TGG | - | chr5.2:65798499-65798518 | None:intergenic | 40.0% | |
| CTCTTGTTGCCTTTGGTTAT+TGG | - | chr5.2:65797751-65797770 | None:intergenic | 40.0% | |
| GTAAGGATCATAGGCAAAGT+TGG | - | chr5.2:65798093-65798112 | None:intergenic | 40.0% | |
| TAGTGTGAGCTTCAGTAATC+TGG | + | chr5.2:65798872-65798891 | MS.gene063627:CDS | 40.0% | |
| TCAGGTAAATTATGCACTCG+GGG | + | chr5.2:65798755-65798774 | MS.gene063627:intron | 40.0% | |
| TCCTTACAGTGTTCCTAACT+GGG | - | chr5.2:65798498-65798517 | None:intergenic | 40.0% | |
| TTCCAGATTGCAATACCTTC+AGG | + | chr5.2:65797791-65797810 | MS.gene063627:CDS | 40.0% | |
| TTTCAAGCACCTTTGTTGGT+AGG | + | chr5.2:65797981-65798000 | MS.gene063627:intron | 40.0% | |
| !! | TCTGGAAGTACCATATCTTC+CGG | - | chr5.2:65797778-65797797 | None:intergenic | 40.0% |
| !!! | CAATTTTAACTTCTTGCGCC+AGG | + | chr5.2:65798112-65798131 | MS.gene063627:CDS | 40.0% |
| ACAATTGTAGCTGGAGTTGG+TGG | + | chr5.2:65798442-65798461 | MS.gene063627:CDS | 45.0% | |
| GCTACAATTGTAGCTGGAGT+TGG | + | chr5.2:65798439-65798458 | MS.gene063627:CDS | 45.0% | |
| GGGATCTGCAATTGTTGTGT+TGG | + | chr5.2:65798313-65798332 | MS.gene063627:CDS | 45.0% | |
| TCCAGCTACAATTGTAGCGT+TGG | - | chr5.2:65798437-65798456 | None:intergenic | 45.0% | |
| TGGGTTCAGTTAAGCAGTCA+AGG | - | chr5.2:65798264-65798283 | None:intergenic | 45.0% | |
| TTAACTTCTTGCGCCAGGTA+TGG | + | chr5.2:65798117-65798136 | MS.gene063627:intron | 45.0% | |
| ! | ACAATTAGTGGGTCCATACC+TGG | - | chr5.2:65798133-65798152 | None:intergenic | 45.0% |
| ! | CAATTGTAGCTGGAGTTGGT+GGG | + | chr5.2:65798443-65798462 | MS.gene063627:CDS | 45.0% |
| !! | CACCTGAAGGTATTGCAATC+TGG | - | chr5.2:65797796-65797815 | None:intergenic | 45.0% |
| GCCAACGCTACAATTGTAGC+TGG | + | chr5.2:65798433-65798452 | MS.gene063627:CDS | 50.0% | |
| GCCCAGTTAGGAACACTGTA+AGG | + | chr5.2:65798494-65798513 | MS.gene063627:intron | 50.0% | |
| !! | GCTACAATTGTAGCGTTGGC+TGG | - | chr5.2:65798433-65798452 | None:intergenic | 50.0% |
| CAAAGGCAACAAGAGCGGAC+CGG | + | chr5.2:65797756-65797775 | MS.gene063627:CDS | 55.0% | |
| CAAGAGCGGACCGGAAGATA+TGG | + | chr5.2:65797765-65797784 | MS.gene063627:CDS | 55.0% | |
| CGGTCCGCTCTTGTTGCCTT+TGG | - | chr5.2:65797758-65797777 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 65797741 | 65798935 | 65797741 | ID=MS.gene063627 |
| chr5.2 | mRNA | 65797741 | 65798935 | 65797741 | ID=MS.gene063627.t1;Parent=MS.gene063627 |
| chr5.2 | exon | 65797741 | 65797812 | 65797741 | ID=MS.gene063627.t1.exon1;Parent=MS.gene063627.t1 |
| chr5.2 | CDS | 65797741 | 65797812 | 65797741 | ID=cds.MS.gene063627.t1;Parent=MS.gene063627.t1 |
| chr5.2 | exon | 65797903 | 65797939 | 65797903 | ID=MS.gene063627.t1.exon2;Parent=MS.gene063627.t1 |
| chr5.2 | CDS | 65797903 | 65797939 | 65797903 | ID=cds.MS.gene063627.t1;Parent=MS.gene063627.t1 |
| chr5.2 | exon | 65798060 | 65798133 | 65798060 | ID=MS.gene063627.t1.exon3;Parent=MS.gene063627.t1 |
| chr5.2 | CDS | 65798060 | 65798133 | 65798060 | ID=cds.MS.gene063627.t1;Parent=MS.gene063627.t1 |
| chr5.2 | exon | 65798241 | 65798334 | 65798241 | ID=MS.gene063627.t1.exon4;Parent=MS.gene063627.t1 |
| chr5.2 | CDS | 65798241 | 65798334 | 65798241 | ID=cds.MS.gene063627.t1;Parent=MS.gene063627.t1 |
| chr5.2 | exon | 65798428 | 65798510 | 65798428 | ID=MS.gene063627.t1.exon5;Parent=MS.gene063627.t1 |
| chr5.2 | CDS | 65798428 | 65798510 | 65798428 | ID=cds.MS.gene063627.t1;Parent=MS.gene063627.t1 |
| chr5.2 | exon | 65798759 | 65798935 | 65798759 | ID=MS.gene063627.t1.exon6;Parent=MS.gene063627.t1 |
| chr5.2 | CDS | 65798759 | 65798935 | 65798759 | ID=cds.MS.gene063627.t1;Parent=MS.gene063627.t1 |
| Gene Sequence |
| Protein sequence |
