AlfalfaGEDB | MS.gene063807

M. sativa cultivar XinJiangDaYe / MS.gene063807

KEGG: K22312
GO: GO:0003674 GO:0003824 GO:0008146 GO:0016740 GO:0016782
PFAM: PF00685.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063807.t1	AET02142.2	87.4	151	19	0	2	152	260	410	4.60E-70	273.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063807.t1	Q8GZ53	53.7	149	68	1	2	149	197	345	6.3e-44	178.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063807.t1	G7LDJ5	87.4	151	19	0	2	152	260	410	3.3e-70	273.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051626	MS.gene063807	0.808089	3.67E-50	-1.69E-46
MS.gene051789	MS.gene063807	0.835997	1.21E-56	-1.69E-46
MS.gene053074	MS.gene063807	0.81879	1.63E-52	-1.69E-46
MS.gene053204	MS.gene063807	0.811939	5.45E-51	-1.69E-46
MS.gene054596	MS.gene063807	0.803172	3.96E-49	-1.69E-46
MS.gene060476	MS.gene063807	0.81111	8.25E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063807.t1	MTR_8g035720	87.417	151	19	0	2	152	260	410	7.71e-90	267
MS.gene063807.t1	MTR_8g040430	78.808	151	32	0	2	152	99	249	2.58e-85	250
MS.gene063807.t1	MTR_8g040430	78.808	151	32	0	2	152	199	349	3.22e-84	251
MS.gene063807.t1	MTR_8g040820	78.378	148	32	0	2	149	99	246	3.20e-81	240
MS.gene063807.t1	MTR_8g040820	78.378	148	32	0	2	149	198	345	7.54e-80	239
MS.gene063807.t1	MTR_8g040490	77.852	149	33	0	2	150	197	345	2.67e-79	239
MS.gene063807.t1	MTR_7g007710	67.550	151	49	0	2	152	186	336	9.53e-73	221
MS.gene063807.t1	MTR_7g007680	68.531	143	45	0	7	149	192	334	2.15e-71	219
MS.gene063807.t1	MTR_3g013500	64.189	148	53	0	2	149	191	338	5.49e-64	199
MS.gene063807.t1	MTR_8g041790	62.585	147	55	0	3	149	180	326	2.20e-63	197
MS.gene063807.t1	MTR_8g040315	80.000	130	25	1	23	152	8	136	3.61e-63	190
MS.gene063807.t1	MTR_8g040250	58.784	148	59	1	2	149	149	294	5.17e-58	182
MS.gene063807.t1	MTR_8g040520	60.284	141	56	0	2	142	195	335	7.88e-57	181
MS.gene063807.t1	MTR_8g040710	56.204	137	60	0	2	138	146	282	2.54e-52	167
MS.gene063807.t1	MTR_8g066220	51.748	143	69	0	6	148	191	333	1.13e-49	162
MS.gene063807.t1	MTR_8g066210	48.252	143	74	0	6	148	176	318	1.43e-46	154
MS.gene063807.t1	MTR_8g066280	47.552	143	75	0	6	148	67	209	4.60e-46	149
MS.gene063807.t1	MTR_8g014380	57.937	126	39	1	2	127	171	282	6.72e-45	148
MS.gene063807.t1	MTR_0071s0010	46.853	143	76	0	6	148	191	333	2.49e-42	143
MS.gene063807.t1	MTR_3g046670	46.853	143	76	0	6	148	191	333	2.49e-42	143
MS.gene063807.t1	MTR_3g434790	46.154	143	77	0	6	148	92	234	2.59e-42	140
MS.gene063807.t1	MTR_3g435130	45.455	143	78	0	6	148	192	334	6.70e-41	139
MS.gene063807.t1	MTR_8g040410	56.522	92	22	1	29	120	72	145	6.46e-28	101
MS.gene063807.t1	MTR_8g066190	41.284	109	46	1	40	148	2	92	8.30e-22	84.0
MS.gene063807.t1	MTR_8g040730	59.574	47	19	0	2	48	150	196	1.46e-13	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063807.t1	AT5G07000	53.691	149	68	1	2	149	197	345	2.59e-54	174
MS.gene063807.t1	AT5G07010	52.055	146	69	1	4	149	213	357	1.48e-53	173
MS.gene063807.t1	AT2G14920	44.366	142	78	1	7	148	193	333	2.04e-43	146
MS.gene063807.t1	AT3G45070	45.775	142	77	0	7	148	182	323	4.79e-43	145
MS.gene063807.t1	AT3G45070	45.775	142	77	0	7	148	187	328	5.73e-43	145
MS.gene063807.t1	AT3G45070	45.775	142	77	0	7	148	214	355	1.15e-42	144
MS.gene063807.t1	AT5G43690	42.958	142	81	0	7	148	190	331	6.60e-42	142
MS.gene063807.t1	AT3G45080	45.455	143	77	1	7	148	187	329	2.43e-41	140
MS.gene063807.t1	AT1G18590	42.857	147	80	1	4	146	195	341	9.36e-41	139
MS.gene063807.t1	AT1G13420	44.056	143	80	0	6	148	189	331	2.81e-40	138
MS.gene063807.t1	AT2G03770	46.479	142	76	0	7	148	181	322	1.69e-39	135
MS.gene063807.t1	AT4G26280	41.791	134	78	0	8	141	169	302	1.71e-38	133
MS.gene063807.t1	AT1G74100	39.041	146	85	1	5	146	188	333	1.93e-36	128
MS.gene063807.t1	AT1G13430	42.754	138	78	1	11	148	215	351	1.31e-34	124
MS.gene063807.t1	AT2G03750	41.259	143	84	0	7	149	209	351	1.29e-33	121
MS.gene063807.t1	AT1G28170	43.262	141	80	0	8	148	186	326	7.59e-32	115
MS.gene063807.t1	AT1G74090	40.136	147	84	1	4	146	199	345	1.16e-28	108
MS.gene063807.t1	AT2G27570	35.252	139	75	1	10	148	150	273	3.50e-28	105
MS.gene063807.t1	AT2G03760	43.972	141	75	1	8	148	188	324	4.17e-28	106

Find 21 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAAAATTAACATCTTCTTTA+AGG	0.077239	8.1:+63459135	None:intergenic
AAGGGCCACCTTTAAATGAT+AGG	0.332985	8.1:+63458850	None:intergenic
GGATCAAACCTATCATTTAA+AGG	0.336571	8.1:-63458858	MS.gene063807:CDS
ACTCAAGAAGAGGAAAATAA+TGG	0.378296	8.1:-63459074	MS.gene063807:CDS
GAAAGGGAGAAATAAGAGAT+TGG	0.401587	8.1:-63458944	MS.gene063807:CDS
TAAGTGCGTATGGACCGTTT+TGG	0.444155	8.1:-63459229	MS.gene063807:CDS
GAATCAAAGGGAAATCAATC+TGG	0.446714	8.1:-63459005	MS.gene063807:CDS
TTCAAGAATAAAACCTTATG+TGG	0.458722	8.1:+63459167	None:intergenic
GAGCATAGAAAGACCACATA+AGG	0.468456	8.1:-63459180	MS.gene063807:CDS
AAAGGGAGAAATAAGAGATT+GGG	0.484916	8.1:-63458943	MS.gene063807:CDS
TCATATGTTGGGTTACTTGA+AGG	0.485292	8.1:-63459204	MS.gene063807:CDS
TGAGAGTATGAAGGAATCAA+AGG	0.522308	8.1:-63459018	MS.gene063807:CDS
GAGAGTATGAAGGAATCAAA+GGG	0.526411	8.1:-63459017	MS.gene063807:CDS
ATGCAGCTTTGAGAGTATGA+AGG	0.542576	8.1:-63459027	MS.gene063807:CDS
AATTCCTTTCACTCAAGAAG+AGG	0.560325	8.1:-63459084	MS.gene063807:CDS
TGTAATGGGATAAGTGCGTA+TGG	0.562143	8.1:-63459239	MS.gene063807:CDS
GAAAGGAATTCCCACAAACT+CGG	0.566637	8.1:+63459097	None:intergenic
CCCAACATATGATCCCAAAA+CGG	0.591413	8.1:+63459215	None:intergenic
ATTTCATCTATGGTTAACAA+AGG	0.592454	8.1:+63459275	None:intergenic
AGAAGAGGAAAATAATGGAG+TGG	0.649746	8.1:-63459069	MS.gene063807:CDS
TCAAACCTATCATTTAAAGG+TGG	0.668795	8.1:-63458855	MS.gene063807:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAATTAACATCTTCTTTA+AGG	+	chr8.1:63459000-63459019	None:intergenic	15.0%
!!!	ATTTTTGAAAAATACTGTAA+TGG	-	chr8.1:63458878-63458897	MS.gene063807:CDS	15.0%
!!!	TTTTTCAAAAATTTCATCTA+TGG	+	chr8.1:63458870-63458889	None:intergenic	15.0%
!!!	TTTTTGAAAAATACTGTAAT+GGG	-	chr8.1:63458879-63458898	MS.gene063807:CDS	15.0%
!!!	AAAAAGTTTTTCTTTCGAAA+GGG	-	chr8.1:63459172-63459191	MS.gene063807:CDS	20.0%
!!!	GATAATTTTTCTATCATTGA+AGG	+	chr8.1:63459229-63459248	None:intergenic	20.0%
!	AGAAAAATTATCCAAAGTCA+TGG	-	chr8.1:63459237-63459256	MS.gene063807:CDS	25.0%
!	ATTTCATCTATGGTTAACAA+AGG	+	chr8.1:63458860-63458879	None:intergenic	25.0%
!	TTCAAGAATAAAACCTTATG+TGG	+	chr8.1:63458968-63458987	None:intergenic	25.0%
!!	GAAAAAGTTTTTCTTTCGAA+AGG	-	chr8.1:63459171-63459190	MS.gene063807:CDS	25.0%
!!	TAATTTTTCTTCCATGACTT+TGG	+	chr8.1:63459251-63459270	None:intergenic	25.0%
	AAAAAAATTGCCGAGTTTGT+GGG	-	chr8.1:63459025-63459044	MS.gene063807:CDS	30.0%
	AAAGGGAGAAATAAGAGATT+GGG	-	chr8.1:63459189-63459208	MS.gene063807:CDS	30.0%
	ACTCAAGAAGAGGAAAATAA+TGG	-	chr8.1:63459058-63459077	MS.gene063807:CDS	30.0%
	GGATCAAACCTATCATTTAA+AGG	-	chr8.1:63459274-63459293	MS.gene063807:CDS	30.0%
	GTCATGGAAGAAAAATTAAG+TGG	-	chr8.1:63459253-63459272	MS.gene063807:CDS	30.0%
	TCAAACCTATCATTTAAAGG+TGG	-	chr8.1:63459277-63459296	MS.gene063807:CDS	30.0%
	AATTCCTTTCACTCAAGAAG+AGG	-	chr8.1:63459048-63459067	MS.gene063807:CDS	35.0%
	CAAAAAAATTGCCGAGTTTG+TGG	-	chr8.1:63459024-63459043	MS.gene063807:CDS	35.0%
	GAAAGGGAGAAATAAGAGAT+TGG	-	chr8.1:63459188-63459207	MS.gene063807:CDS	35.0%
	GAATCAAAGGGAAATCAATC+TGG	-	chr8.1:63459127-63459146	MS.gene063807:CDS	35.0%
	TCATATGTTGGGTTACTTGA+AGG	-	chr8.1:63458928-63458947	MS.gene063807:CDS	35.0%
!	AGAAGAGGAAAATAATGGAG+TGG	-	chr8.1:63459063-63459082	MS.gene063807:CDS	35.0%
!	GAGAGTATGAAGGAATCAAA+GGG	-	chr8.1:63459115-63459134	MS.gene063807:CDS	35.0%
!	TGAGAGTATGAAGGAATCAA+AGG	-	chr8.1:63459114-63459133	MS.gene063807:CDS	35.0%
!	TTTTCCTCTTCTTGAGTGAA+AGG	+	chr8.1:63459055-63459074	None:intergenic	35.0%
	AAGGGCCACCTTTAAATGAT+AGG	+	chr8.1:63459285-63459304	None:intergenic	40.0%
	CCCAACATATGATCCCAAAA+CGG	+	chr8.1:63458920-63458939	None:intergenic	40.0%
	GAAAGGAATTCCCACAAACT+CGG	+	chr8.1:63459038-63459057	None:intergenic	40.0%
	GAGCATAGAAAGACCACATA+AGG	-	chr8.1:63458952-63458971	MS.gene063807:CDS	40.0%
!	TGTAATGGGATAAGTGCGTA+TGG	-	chr8.1:63458893-63458912	MS.gene063807:CDS	40.0%
!!	ATGCAGCTTTGAGAGTATGA+AGG	-	chr8.1:63459105-63459124	MS.gene063807:CDS	40.0%
!!!	ACCGTTTTGGGATCATATGT+TGG	-	chr8.1:63458916-63458935	MS.gene063807:CDS	40.0%
!!!	CCGTTTTGGGATCATATGTT+GGG	-	chr8.1:63458917-63458936	MS.gene063807:CDS	40.0%
!	AAGTGCGTATGGACCGTTTT+GGG	-	chr8.1:63458904-63458923	MS.gene063807:CDS	45.0%
!	TAAGTGCGTATGGACCGTTT+TGG	-	chr8.1:63458903-63458922	MS.gene063807:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	63458848	63459306	63458848	ID=MS.gene063807
chr8.1	mRNA	63458848	63459306	63458848	ID=MS.gene063807.t1;Parent=MS.gene063807
chr8.1	exon	63458848	63459306	63458848	ID=MS.gene063807.t1.exon1;Parent=MS.gene063807.t1
chr8.1	CDS	63458848	63459306	63458848	ID=cds.MS.gene063807.t1;Parent=MS.gene063807.t1

Gene Sequence

>MS.gene063807

ATGTCTTTACCTTTGTTAACCATAGATGAAATTTTTGAAAAATACTGTAATGGGATAAGTGCGTATGGACCGTTTTGGGATCATATGTTGGGTTACTTGAAGGAGAGCATAGAAAGACCACATAAGGTTTTATTCTTGAAATATGAAGACCTTAAAGAAGATGTTAATTTTAACATCAAAAAAATTGCCGAGTTTGTGGGAATTCCTTTCACTCAAGAAGAGGAAAATAATGGAGTGGTTGAAAATATTATCAAGTTATGCAGCTTTGAGAGTATGAAGGAATCAAAGGGAAATCAATCTGGAACTGTATCAACAATCGTTGAGAAAAAGTTTTTCTTTCGAAAGGGAGAAATAAGAGATTGGGTAAATTACTTTTCTCCTTCAATGATAGAAAAATTATCCAAAGTCATGGAAGAAAAATTAAGTGGATCAAACCTATCATTTAAAGGTGGCCCTTAA

Protein sequence

>MS.gene063807.t1

MSLPLLTIDEIFEKYCNGISAYGPFWDHMLGYLKESIERPHKVLFLKYEDLKEDVNFNIKKIAEFVGIPFTQEEENNGVVENIIKLCSFESMKESKGNQSGTVSTIVEKKFFFRKGEIRDWVNYFSPSMIEKLSKVMEEKLSGSNLSFKGGP