AlfalfaGEDB | MS.gene95103

M. sativa cultivar XinJiangDaYe / MS.gene95103

KEGG: K03955
GO: GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009987 GO:0016053 GO:0019752 GO:0032787 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0046394 GO:0071704 GO:0072330 GO:1901576
PFAM: PF00550.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95103.t1	PNY14930.1	95.2	124	6	0	1	124	1	124	5.10E-59	236.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95103.t1	Q9FGJ4	56.7	127	51	1	1	123	1	127	2.5e-30	132.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95103.t1	A0A2K3PI09	95.2	124	6	0	1	124	1	124	3.7e-59	236.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051860	MS.gene95103	0.835351	1.77E-56	-1.69E-46
MS.gene052011	MS.gene95103	-0.801955	7.05E-49	-1.69E-46
MS.gene052135	MS.gene95103	0.803801	2.93E-49	-1.69E-46
MS.gene052253	MS.gene95103	0.818649	1.76E-52	-1.69E-46
MS.gene054011	MS.gene95103	0.80473	1.88E-49	-1.69E-46
MS.gene054183	MS.gene95103	0.822921	1.83E-53	-1.69E-46
MS.gene05856	MS.gene95103	0.803794	2.94E-49	-1.69E-46
MS.gene059420	MS.gene95103	0.82603	3.39E-54	-1.69E-46
MS.gene061049	MS.gene95103	0.804656	1.95E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene95103	MS.gene028119	PPI
MS.gene95103	MS.gene72802	PPI
MS.gene064261	MS.gene95103	PPI
MS.gene95103	MS.gene033495	PPI
MS.gene95103	MS.gene033497	PPI
MS.gene95103	MS.gene024056	PPI
MS.gene52597	MS.gene95103	PPI
MS.gene95103	MS.gene058580	PPI
MS.gene95103	MS.gene88356	PPI
MS.gene95103	MS.gene82126	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95103.t1	MTR_4g133928	94.355	124	7	0	1	124	1	124	8.31e-83	238
MS.gene95103.t1	MTR_0446s0010	88.889	108	9	1	1	108	1	105	9.34e-65	192
MS.gene95103.t1	MTR_7g080390	50.649	77	38	0	47	123	54	130	2.57e-21	82.8
MS.gene95103.t1	MTR_7g080760	48.649	74	38	0	47	120	43	116	2.59e-21	82.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene95103.t1	AT5G47630	56.693	127	51	1	1	123	1	127	1.04e-42	137
MS.gene95103.t1	AT5G47630	56.693	127	51	1	1	123	1	127	1.04e-42	137
MS.gene95103.t1	AT5G47630	56.693	127	51	1	1	123	1	127	1.04e-42	137
MS.gene95103.t1	AT1G65290	50.649	77	38	0	47	123	50	126	3.40e-21	82.4
MS.gene95103.t1	AT2G44620	41.000	100	46	1	17	116	29	115	6.13e-21	81.6

Find 18 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATAGGGTTGAGCTTATTA+TGG	0.307345	8.2:-778081	MS.gene95103:CDS
AGCTTATCTGCTTTCTCATC+AGG	0.361227	8.2:+778029	None:intergenic
CATAATCACATTATCATCCT+TGG	0.439969	8.2:+778375	None:intergenic
GGACTTAAACCTGGACAGCT+TGG	0.459469	8.2:-778102	MS.gene95103:CDS
TGATTATGCATTTGAGATGT+TGG	0.460487	8.2:-778360	MS.gene95103:CDS
TGAGAAGGTCAACTGAAAGA+TGG	0.475010	8.2:-778405	MS.gene95103:CDS
GTCTAGTATCGGATCAGAGC+TGG	0.475971	8.2:+778321	None:intergenic
AGATGTTGGTGCTCTTCAGC+AGG	0.487417	8.2:-778346	MS.gene95103:CDS
CAACCCTATCCAAGCTGTCC+AGG	0.498453	8.2:+778093	None:intergenic
TAAACCTGGACAGCTTGGAT+AGG	0.517739	8.2:-778097	MS.gene95103:CDS
TCTTGACCAGTCTGATTACT+CGG	0.521850	8.2:+778299	None:intergenic
AAACCTGGACAGCTTGGATA+GGG	0.522123	8.2:-778096	MS.gene95103:CDS
CTGATTACTCGGTCTAGTAT+CGG	0.536163	8.2:+778310	None:intergenic
GGCTATGTACTTTGCAACAT+CGG	0.551029	8.2:+777995	None:intergenic
TTTCTCATCAGGAATTTCGA+TGG	0.580152	8.2:+778040	None:intergenic
TGATAAAATTAATGCAGCAA+AGG	0.580568	8.2:-778272	MS.gene95103:intron
TAAGTCATGTGAATTTGAGA+AGG	0.613186	8.2:-778420	MS.gene95103:CDS
ACTAGACCGAGTAATCAGAC+TGG	0.680991	8.2:-778305	MS.gene95103:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTGGTATATATTTTTGTGT+AGG	-	chr8.2:778266-778285	MS.gene95103:intron	20.0%
!!!	TATTTTCTTTTGACTATAAC+TGG	+	chr8.2:778174-778193	None:intergenic	20.0%
!	TGATAAAATTAATGCAGCAA+AGG	-	chr8.2:778144-778163	MS.gene95103:intron	25.0%
	CATAATCACATTATCATCCT+TGG	+	chr8.2:778044-778063	None:intergenic	30.0%
	TAAGTCATGTGAATTTGAGA+AGG	-	chr8.2:777996-778015	MS.gene95103:CDS	30.0%
	TTCACGTGTAGATTAAGTAT+TGG	-	chr8.2:778248-778267	MS.gene95103:intron	30.0%
!	TGATTATGCATTTGAGATGT+TGG	-	chr8.2:778056-778075	MS.gene95103:CDS	30.0%
!	TTTTCAAAAGGACTTAAACC+TGG	-	chr8.2:778305-778324	MS.gene95103:CDS	30.0%
	TTATCAGTGTTACACAGTCT+GGG	-	chr8.2:778217-778236	MS.gene95103:intron	35.0%
	TTTATCAGTGTTACACAGTC+TGG	-	chr8.2:778216-778235	MS.gene95103:intron	35.0%
	TTTCTCATCAGGAATTTCGA+TGG	+	chr8.2:778379-778398	None:intergenic	35.0%
!	GTCAACTGAAAGATGGTTTT+TGG	-	chr8.2:778018-778037	MS.gene95103:CDS	35.0%
!!	TGAAGCAGCTGATTTTCAAA+AGG	-	chr8.2:778293-778312	MS.gene95103:CDS	35.0%
!!!	AATTTTTTGGTCAGCTTCAG+AGG	+	chr8.2:778445-778464	None:intergenic	35.0%
	AGCTTATCTGCTTTCTCATC+AGG	+	chr8.2:778390-778409	None:intergenic	40.0%
	CTGATTACTCGGTCTAGTAT+CGG	+	chr8.2:778109-778128	None:intergenic	40.0%
	GGATAGGGTTGAGCTTATTA+TGG	-	chr8.2:778335-778354	MS.gene95103:CDS	40.0%
	GGCTATGTACTTTGCAACAT+CGG	+	chr8.2:778424-778443	None:intergenic	40.0%
	TCTTGACCAGTCTGATTACT+CGG	+	chr8.2:778120-778139	None:intergenic	40.0%
	TGAGAAGGTCAACTGAAAGA+TGG	-	chr8.2:778011-778030	MS.gene95103:CDS	40.0%
!!!	TGAAAGATGGTTTTTGGCCA+AGG	-	chr8.2:778024-778043	MS.gene95103:CDS	40.0%
	AAACCTGGACAGCTTGGATA+GGG	-	chr8.2:778320-778339	MS.gene95103:CDS	45.0%
	ACTAGACCGAGTAATCAGAC+TGG	-	chr8.2:778111-778130	MS.gene95103:CDS	45.0%
	TAAACCTGGACAGCTTGGAT+AGG	-	chr8.2:778319-778338	MS.gene95103:CDS	45.0%
	GGACTTAAACCTGGACAGCT+TGG	-	chr8.2:778314-778333	MS.gene95103:CDS	50.0%
!	AGATGTTGGTGCTCTTCAGC+AGG	-	chr8.2:778070-778089	MS.gene95103:CDS	50.0%
!	GTCTAGTATCGGATCAGAGC+TGG	+	chr8.2:778098-778117	None:intergenic	50.0%
	CAACCCTATCCAAGCTGTCC+AGG	+	chr8.2:778326-778345	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	777971	778467	777971	ID=MS.gene95103
chr8.2	mRNA	777971	778467	777971	ID=MS.gene95103.t1;Parent=MS.gene95103
chr8.2	exon	778273	778467	778273	ID=MS.gene95103.t1.exon1;Parent=MS.gene95103.t1
chr8.2	CDS	778273	778467	778273	ID=cds.MS.gene95103.t1;Parent=MS.gene95103.t1
chr8.2	exon	777971	778150	777971	ID=MS.gene95103.t1.exon2;Parent=MS.gene95103.t1
chr8.2	CDS	777971	778150	777971	ID=cds.MS.gene95103.t1;Parent=MS.gene95103.t1

Gene Sequence

>MS.gene95103

ATGCAAAGCATAAAGAAATCTATACTAAGTCATGTGAATTTGAGAAGGTCAACTGAAAGATGGTTTTTGGCCAAGGATGATAATGTGATTATGCATTTGAGATGTTGGTGCTCTTCAGCAGGTGCCAGCTCTGATCCGATACTAGACCGAGTAATCAGACTGGTCAAGAAATATGATAAAATTAATGCAGCAAAGGTATTCCAGTTATAGTCAAAAGAAAATACTACATTTTGTTTATGATTATCTTTATCAGTGTTACACAGTCTGGGATTCTGTTTTCACGTGTAGATTAAGTATTGGTATATATTTTTGTGTAGGTTACTGAAGCAGCTGATTTTCAAAAGGACTTAAACCTGGACAGCTTGGATAGGGTTGAGCTTATTATGGCTCTTGAAGAAGAATTTTCCATCGAAATTCCTGATGAGAAAGCAGATAAGCTTACTTGCTGTACCGATGTTGCAAAGTACATAGCCTCTGAAGCTGACCAAAAAATTTAG

Protein sequence

>MS.gene95103.t1

MQSIKKSILSHVNLRRSTERWFLAKDDNVIMHLRCWCSSAGASSDPILDRVIRLVKKYDKINAAKVTEAADFQKDLNLDSLDRVELIMALEEEFSIEIPDEKADKLTCCTDVAKYIASEADQKI