AlfalfaGEDB | MS.gene43422

M. sativa cultivar XinJiangDaYe / MS.gene43422

KEGG: K03955
GO: GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009987 GO:0016053 GO:0019752 GO:0032787 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0046394 GO:0071704 GO:0072330 GO:1901576
PFAM: PF00550.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43422.t1	PNY14930.1	95.2	124	6	0	1	124	1	124	5.10E-59	236.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43422.t1	Q9FGJ4	56.7	127	51	1	1	123	1	127	2.5e-30	132.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43422.t1	A0A2K3PI09	95.2	124	6	0	1	124	1	124	3.7e-59	236.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051860	MS.gene43422	0.841539	4.47E-58	-1.69E-46
MS.gene052011	MS.gene43422	-0.81107	8.41E-51	-1.69E-46
MS.gene052253	MS.gene43422	0.803325	3.68E-49	-1.69E-46
MS.gene054010	MS.gene43422	0.808718	2.70E-50	-1.69E-46
MS.gene054011	MS.gene43422	0.810386	1.18E-50	-1.69E-46
MS.gene054012	MS.gene43422	0.802512	5.42E-49	-1.69E-46
MS.gene054013	MS.gene43422	0.802952	4.39E-49	-1.69E-46
MS.gene054173	MS.gene43422	0.81162	6.39E-51	-1.69E-46
MS.gene054183	MS.gene43422	0.832198	1.08E-55	-1.69E-46
MS.gene054473	MS.gene43422	0.800881	1.17E-48	-1.69E-46
MS.gene055604	MS.gene43422	0.803279	3.76E-49	-1.69E-46
MS.gene055666	MS.gene43422	0.802259	6.11E-49	-1.69E-46
MS.gene057469	MS.gene43422	0.815157	1.07E-51	-1.69E-46
MS.gene057476	MS.gene43422	0.802571	5.27E-49	-1.69E-46
MS.gene059420	MS.gene43422	0.842766	2.12E-58	-1.69E-46
MS.gene06037	MS.gene43422	0.806578	7.69E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene064261	MS.gene43422	PPI
MS.gene43422	MS.gene82126	PPI
MS.gene43422	MS.gene024056	PPI
MS.gene43422	MS.gene058580	PPI
MS.gene43422	MS.gene88356	PPI
MS.gene43422	MS.gene028119	PPI
MS.gene52597	MS.gene43422	PPI
MS.gene43422	MS.gene72802	PPI
MS.gene43422	MS.gene033495	PPI
MS.gene43422	MS.gene033497	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43422.t1	MTR_4g133928	94.355	124	7	0	1	124	1	124	8.31e-83	238
MS.gene43422.t1	MTR_0446s0010	88.889	108	9	1	1	108	1	105	9.34e-65	192
MS.gene43422.t1	MTR_7g080390	50.649	77	38	0	47	123	54	130	2.57e-21	82.8
MS.gene43422.t1	MTR_7g080760	48.649	74	38	0	47	120	43	116	2.59e-21	82.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43422.t1	AT5G47630	56.693	127	51	1	1	123	1	127	1.04e-42	137
MS.gene43422.t1	AT5G47630	56.693	127	51	1	1	123	1	127	1.04e-42	137
MS.gene43422.t1	AT5G47630	56.693	127	51	1	1	123	1	127	1.04e-42	137
MS.gene43422.t1	AT1G65290	50.649	77	38	0	47	123	50	126	3.40e-21	82.4
MS.gene43422.t1	AT2G44620	41.000	100	46	1	17	116	29	115	6.13e-21	81.6

Find 18 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATAGGGTTGAGCTTATTA+TGG	0.307345	8.4:-824120	MS.gene43422:CDS
AGCTTATCTGCTTTCTCATC+AGG	0.361227	8.4:+824068	None:intergenic
CATAATCACATTATCATCCT+TGG	0.439969	8.4:+824414	None:intergenic
GGACTTAAACCTGGACAGCT+TGG	0.459469	8.4:-824141	MS.gene43422:CDS
TGATTATGCATTTGAGATGT+TGG	0.460487	8.4:-824399	MS.gene43422:CDS
TGAGAAGGTCAACTGAAAGA+TGG	0.475010	8.4:-824444	MS.gene43422:CDS
GTCTAGTATCGGATCAGAGC+TGG	0.475971	8.4:+824360	None:intergenic
AGATGTTGGTGCTCTTCAGC+AGG	0.487417	8.4:-824385	MS.gene43422:CDS
CAACCCTATCCAAGCTGTCC+AGG	0.498453	8.4:+824132	None:intergenic
TAAACCTGGACAGCTTGGAT+AGG	0.517739	8.4:-824136	MS.gene43422:CDS
TCTTGACCAGTCTGATTACT+CGG	0.521850	8.4:+824338	None:intergenic
AAACCTGGACAGCTTGGATA+GGG	0.522123	8.4:-824135	MS.gene43422:CDS
CTGATTACTCGGTCTAGTAT+CGG	0.536163	8.4:+824349	None:intergenic
GGCTATGTACTTTGCAACAT+CGG	0.551029	8.4:+824034	None:intergenic
TTTCTCATCAGGAATTTCGA+TGG	0.580152	8.4:+824079	None:intergenic
TGATAAAATTAATGCAGCAA+AGG	0.580568	8.4:-824311	MS.gene43422:intron
TAAGTCATGTGAATTTGAGA+AGG	0.613186	8.4:-824459	MS.gene43422:CDS
ACTAGACCGAGTAATCAGAC+TGG	0.680991	8.4:-824344	MS.gene43422:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTGGTATATATTTTTGTGT+AGG	-	chr8.4:824305-824324	MS.gene43422:intron	20.0%
!!!	TATTTTCTTTTGACTATAAC+TGG	+	chr8.4:824213-824232	None:intergenic	20.0%
!	TGATAAAATTAATGCAGCAA+AGG	-	chr8.4:824183-824202	MS.gene43422:intron	25.0%
	CATAATCACATTATCATCCT+TGG	+	chr8.4:824083-824102	None:intergenic	30.0%
	TAAGTCATGTGAATTTGAGA+AGG	-	chr8.4:824035-824054	MS.gene43422:CDS	30.0%
	TTCACGTGTAGATTAAGTAT+TGG	-	chr8.4:824287-824306	MS.gene43422:intron	30.0%
!	TGATTATGCATTTGAGATGT+TGG	-	chr8.4:824095-824114	MS.gene43422:CDS	30.0%
!	TTTTCAAAAGGACTTAAACC+TGG	-	chr8.4:824344-824363	MS.gene43422:CDS	30.0%
	TTATCAGTGTTACACAGTCT+GGG	-	chr8.4:824256-824275	MS.gene43422:intron	35.0%
	TTTATCAGTGTTACACAGTC+TGG	-	chr8.4:824255-824274	MS.gene43422:intron	35.0%
	TTTCTCATCAGGAATTTCGA+TGG	+	chr8.4:824418-824437	None:intergenic	35.0%
!	GTCAACTGAAAGATGGTTTT+TGG	-	chr8.4:824057-824076	MS.gene43422:CDS	35.0%
!!	TGAAGCAGCTGATTTTCAAA+AGG	-	chr8.4:824332-824351	MS.gene43422:CDS	35.0%
!!!	AATTTTTTGGTCAGCTTCAG+AGG	+	chr8.4:824484-824503	None:intergenic	35.0%
	AGCTTATCTGCTTTCTCATC+AGG	+	chr8.4:824429-824448	None:intergenic	40.0%
	CTGATTACTCGGTCTAGTAT+CGG	+	chr8.4:824148-824167	None:intergenic	40.0%
	GGATAGGGTTGAGCTTATTA+TGG	-	chr8.4:824374-824393	MS.gene43422:CDS	40.0%
	GGCTATGTACTTTGCAACAT+CGG	+	chr8.4:824463-824482	None:intergenic	40.0%
	TCTTGACCAGTCTGATTACT+CGG	+	chr8.4:824159-824178	None:intergenic	40.0%
	TGAGAAGGTCAACTGAAAGA+TGG	-	chr8.4:824050-824069	MS.gene43422:CDS	40.0%
!!!	TGAAAGATGGTTTTTGGCCA+AGG	-	chr8.4:824063-824082	MS.gene43422:CDS	40.0%
	AAACCTGGACAGCTTGGATA+GGG	-	chr8.4:824359-824378	MS.gene43422:CDS	45.0%
	ACTAGACCGAGTAATCAGAC+TGG	-	chr8.4:824150-824169	MS.gene43422:CDS	45.0%
	TAAACCTGGACAGCTTGGAT+AGG	-	chr8.4:824358-824377	MS.gene43422:CDS	45.0%
	GGACTTAAACCTGGACAGCT+TGG	-	chr8.4:824353-824372	MS.gene43422:CDS	50.0%
!	AGATGTTGGTGCTCTTCAGC+AGG	-	chr8.4:824109-824128	MS.gene43422:CDS	50.0%
!	GTCTAGTATCGGATCAGAGC+TGG	+	chr8.4:824137-824156	None:intergenic	50.0%
	CAACCCTATCCAAGCTGTCC+AGG	+	chr8.4:824365-824384	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	824010	824506	824010	ID=MS.gene43422
chr8.4	mRNA	824010	824506	824010	ID=MS.gene43422.t1;Parent=MS.gene43422
chr8.4	exon	824312	824506	824312	ID=MS.gene43422.t1.exon1;Parent=MS.gene43422.t1
chr8.4	CDS	824312	824506	824312	ID=cds.MS.gene43422.t1;Parent=MS.gene43422.t1
chr8.4	exon	824010	824189	824010	ID=MS.gene43422.t1.exon2;Parent=MS.gene43422.t1
chr8.4	CDS	824010	824189	824010	ID=cds.MS.gene43422.t1;Parent=MS.gene43422.t1

Gene Sequence

>MS.gene43422

ATGCAAAGCATAAAGAAATCTATACTAAGTCATGTGAATTTGAGAAGGTCAACTGAAAGATGGTTTTTGGCCAAGGATGATAATGTGATTATGCATTTGAGATGTTGGTGCTCTTCAGCAGGTGCCAGCTCTGATCCGATACTAGACCGAGTAATCAGACTGGTCAAGAAATATGATAAAATTAATGCAGCAAAGGTATTCCAGTTATAGTCAAAAGAAAATACTACATTTTGTTTATGATTATCTTTATCAGTGTTACACAGTCTGGGATTCTGTTTTCACGTGTAGATTAAGTATTGGTATATATTTTTGTGTAGGTTACTGAAGCAGCTGATTTTCAAAAGGACTTAAACCTGGACAGCTTGGATAGGGTTGAGCTTATTATGGCTCTTGAAGAAGAATTTTCCATCGAAATTCCTGATGAGAAAGCAGATAAGCTTACTTGCTGTACCGATGTTGCAAAGTACATAGCCTCTGAAGCTGACCAAAAAATTTAG

Protein sequence

>MS.gene43422.t1

MQSIKKSILSHVNLRRSTERWFLAKDDNVIMHLRCWCSSAGASSDPILDRVIRLVKKYDKINAAKVTEAADFQKDLNLDSLDRVELIMALEEEFSIEIPDEKADKLTCCTDVAKYIASEADQKI