Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064930.t1 | XP_003590190.1 | 99.1 | 459 | 4 | 0 | 1 | 459 | 1 | 459 | 4.40E-265 | 923.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064930.t1 | Q94AR8 | 78.6 | 463 | 92 | 3 | 3 | 459 | 2 | 463 | 9.5e-216 | 750.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064930.t1 | G7I6Q7 | 99.1 | 459 | 4 | 0 | 1 | 459 | 1 | 459 | 3.2e-265 | 923.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene059965 | MS.gene064930 | PPI |
| MS.gene064930 | MS.gene011362 | PPI |
| MS.gene064930 | MS.gene48297 | PPI |
| MS.gene033323 | MS.gene064930 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064930.t1 | MTR_1g045750 | 99.129 | 459 | 4 | 0 | 1 | 459 | 1 | 459 | 0.0 | 952 |
| MS.gene064930.t1 | MTR_1g073200 | 68.476 | 479 | 122 | 6 | 8 | 459 | 5 | 481 | 0.0 | 673 |
| MS.gene064930.t1 | MTR_1g045760 | 48.000 | 125 | 46 | 1 | 107 | 231 | 52 | 157 | 6.50e-27 | 106 |
| MS.gene064930.t1 | MTR_4g048190 | 27.488 | 211 | 134 | 5 | 166 | 360 | 264 | 471 | 2.96e-15 | 79.0 |
| MS.gene064930.t1 | MTR_2g088360 | 27.907 | 215 | 128 | 6 | 166 | 360 | 264 | 471 | 6.72e-15 | 77.8 |
| MS.gene064930.t1 | MTR_5g022940 | 27.907 | 215 | 128 | 6 | 166 | 360 | 184 | 391 | 1.63e-13 | 73.6 |
| MS.gene064930.t1 | MTR_5g022940 | 27.907 | 215 | 128 | 6 | 166 | 360 | 80 | 287 | 1.73e-13 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064930.t1 | AT4G13430 | 78.618 | 463 | 92 | 3 | 3 | 459 | 2 | 463 | 0.0 | 777 |
| MS.gene064930.t1 | AT4G26970 | 25.000 | 288 | 182 | 8 | 108 | 365 | 206 | 489 | 1.54e-15 | 80.1 |
| MS.gene064930.t1 | AT2G05710 | 27.488 | 211 | 134 | 6 | 166 | 360 | 275 | 482 | 9.53e-15 | 77.4 |
| MS.gene064930.t1 | AT4G35830 | 23.134 | 268 | 176 | 6 | 122 | 360 | 21 | 287 | 1.10e-12 | 70.9 |
| MS.gene064930.t1 | AT4G35830 | 23.134 | 268 | 176 | 6 | 122 | 360 | 124 | 390 | 1.24e-12 | 70.9 |
Find 112 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACACCTGGGGATAATATT+TGG | 0.139762 | 1.4:+30359661 | MS.gene064930:CDS |
| TCATTTACCTTACAGATTAT+TGG | 0.189248 | 1.4:+30362407 | MS.gene064930:intron |
| TAGATTTGATGTCTCAAAAT+TGG | 0.219082 | 1.4:+30366694 | MS.gene064930:CDS |
| GTTTGTGGTCCTGGTTCTAT+TGG | 0.237513 | 1.4:+30359714 | MS.gene064930:CDS |
| TGCGGGCATAAGTATCTTTC+GGG | 0.251693 | 1.4:-30367619 | None:intergenic |
| TCCTAGCTGTGGTGCTTGTT+TGG | 0.267456 | 1.4:+30367592 | MS.gene064930:CDS |
| TTGCAGACCTTCTGTGTTGC+AGG | 0.279369 | 1.4:-30367364 | None:intergenic |
| GTTCTTTCTGCAGGTTCTCT+TGG | 0.284191 | 1.4:+30361114 | MS.gene064930:intron |
| GCTTGCTGGTCACTATATAC+TGG | 0.339417 | 1.4:-30366132 | None:intergenic |
| TTCTTTCTGCAGGTTCTCTT+GGG | 0.341117 | 1.4:+30361115 | MS.gene064930:intron |
| ATGCGGGCATAAGTATCTTT+CGG | 0.341407 | 1.4:-30367620 | None:intergenic |
| CCTAGCTGTGGTGCTTGTTT+GGG | 0.346701 | 1.4:+30367593 | MS.gene064930:CDS |
| TGGCAAACACCTTTGTAATC+TGG | 0.349547 | 1.4:-30360972 | None:intergenic |
| ACTTGTACTGCTGGAGCATT+TGG | 0.355022 | 1.4:+30361151 | MS.gene064930:CDS |
| AGTCTTCCTGTCCCTGGATC+TGG | 0.355783 | 1.4:+30367515 | MS.gene064930:CDS |
| TATTTATATGTAATGCTATC+TGG | 0.356860 | 1.4:-30362679 | None:intergenic |
| ACTTTGTCTACCTTGCTTGC+TGG | 0.358067 | 1.4:-30366146 | None:intergenic |
| TACACCTGGGGATAATATTT+GGG | 0.359970 | 1.4:+30359662 | MS.gene064930:CDS |
| TTTGTGGTCCTGGTTCTATT+GGG | 0.370313 | 1.4:+30359715 | MS.gene064930:CDS |
| GCATTTGGTCAATTTGCAAC+AGG | 0.376621 | 1.4:+30361166 | MS.gene064930:CDS |
| ACTCATGATGTTTGTGGTCC+TGG | 0.386188 | 1.4:+30359705 | MS.gene064930:CDS |
| TTGCTCAGGAAGGTCATTGC+AGG | 0.404340 | 1.4:+30361003 | MS.gene064930:CDS |
| CAGCGTCAACCTTCTTCAAC+TGG | 0.418217 | 1.4:+30359482 | MS.gene064930:CDS |
| GACTATGACTGAGAAGATAA+TGG | 0.419716 | 1.4:+30359614 | MS.gene064930:CDS |
| CCCCGCACCCAACACAAAAC+CGG | 0.419745 | 1.4:-30361206 | None:intergenic |
| TGCAACATGGTTGTTGAAGC+TGG | 0.422479 | 1.4:+30362634 | MS.gene064930:CDS |
| TCTGTATTTGCAGGGTAAAA+AGG | 0.423632 | 1.4:+30367318 | MS.gene064930:intron |
| TCCAACTTTGAGATTTGTAA+TGG | 0.425112 | 1.4:+30362263 | MS.gene064930:CDS |
| CTTGCCAAAGCACGGTTATC+CGG | 0.433090 | 1.4:-30366840 | None:intergenic |
| TCCATTACAAATCTCAAAGT+TGG | 0.438795 | 1.4:-30362264 | None:intergenic |
| GGTCAATTTGCAACAGGGAT+TGG | 0.439036 | 1.4:+30361172 | MS.gene064930:CDS |
| TAAACTAATCGAACCTCTCC+TGG | 0.452154 | 1.4:-30361026 | None:intergenic |
| TTGCCACATCGCTCTTGCTC+AGG | 0.452572 | 1.4:+30360989 | MS.gene064930:CDS |
| ATGAAGAAAGTACCTTGGTC+TGG | 0.459257 | 1.4:-30359276 | None:intergenic |
| TCACTATATACTGGTTCATA+TGG | 0.463569 | 1.4:-30366123 | None:intergenic |
| CAGGAAGGTCATTGCAGGCC+AGG | 0.469342 | 1.4:+30361008 | MS.gene064930:CDS |
| CAAGTCTTACCACCAGATCC+AGG | 0.471556 | 1.4:-30367527 | None:intergenic |
| GTCTGGAAGAAGGAGGTTGA+AGG | 0.473481 | 1.4:-30359259 | None:intergenic |
| AGCTGTGGTGCTTGTTTGGG+TGG | 0.475059 | 1.4:+30367596 | MS.gene064930:CDS |
| TTCACCGGATAACCGTGCTT+TGG | 0.476151 | 1.4:+30366836 | MS.gene064930:CDS |
| TTGATGACTCATGATGTTTG+TGG | 0.477394 | 1.4:+30359699 | MS.gene064930:CDS |
| GATTCTCACACTTGTACTGC+TGG | 0.482639 | 1.4:+30361142 | MS.gene064930:CDS |
| CATCGCTTGTGAATATATAG+TGG | 0.485083 | 1.4:-30360747 | None:intergenic |
| GTGTATAGTCTTCCTGTCCC+TGG | 0.486113 | 1.4:+30367509 | MS.gene064930:CDS |
| AATGGAATTTCAGGTTTGGA+TGG | 0.486289 | 1.4:+30367484 | MS.gene064930:intron |
| TCACAGATATTTGAAGAAGC+TGG | 0.493068 | 1.4:+30367551 | MS.gene064930:CDS |
| ACTTTGAGATTTGTAATGGA+TGG | 0.496494 | 1.4:+30362267 | MS.gene064930:CDS |
| AATGTTGCAGCCTCATTCAC+CGG | 0.497013 | 1.4:+30366821 | MS.gene064930:intron |
| AAAGATGCTAGGAAAGAAGC+AGG | 0.500343 | 1.4:-30367664 | None:intergenic |
| CTTGTGAATATATAGTGGTC+AGG | 0.502316 | 1.4:-30360742 | None:intergenic |
| TGCAGACCTTCTGTGTTGCA+GGG | 0.506636 | 1.4:-30367363 | None:intergenic |
| GCCAAGTTATTTGCTTGCCA+AGG | 0.519501 | 1.4:+30362296 | MS.gene064930:CDS |
| ATGGCGAAAGCTTCTGAGAA+GGG | 0.519753 | 1.4:+30359633 | MS.gene064930:CDS |
| GTAGGTTTGGGATCGTGAAA+AGG | 0.519967 | 1.4:+30360708 | MS.gene064930:intron |
| GCACCACAGCTAGGACTAGC+AGG | 0.520368 | 1.4:-30367584 | None:intergenic |
| GGGATTGGTAATACTGATGC+CGG | 0.521210 | 1.4:+30361187 | MS.gene064930:CDS |
| TTGTGACATCGTTGAGAAGA+TGG | 0.523423 | 1.4:-30359410 | None:intergenic |
| TGCCCGCATGAATGAACCTC+AGG | 0.527832 | 1.4:+30367634 | MS.gene064930:CDS |
| TACTTGCAGAATCAAATCCT+TGG | 0.528971 | 1.4:-30362313 | None:intergenic |
| CAAACTGATCCAGTTGAAGA+AGG | 0.529297 | 1.4:-30359491 | None:intergenic |
| TGCACTCTCTTGCCAAAGCA+CGG | 0.532465 | 1.4:-30366848 | None:intergenic |
| GAGAAGGGGCATGTTACACC+TGG | 0.536724 | 1.4:+30359648 | MS.gene064930:CDS |
| AAGTACCTTGGTCTGGAAGA+AGG | 0.538243 | 1.4:-30359269 | None:intergenic |
| TACAAATCAATGGAGTTCGT+TGG | 0.543312 | 1.4:+30362455 | MS.gene064930:CDS |
| TCCTTGGCAAGCAAATAACT+TGG | 0.546758 | 1.4:-30362297 | None:intergenic |
| TGCCTGAGGTTCATTCATGC+GGG | 0.549238 | 1.4:-30367636 | None:intergenic |
| ATTGGAGCCACTGGTTGCCA+AGG | 0.549517 | 1.4:+30366712 | MS.gene064930:CDS |
| TATATAGTGACCAGCAAGCA+AGG | 0.550703 | 1.4:+30366136 | MS.gene064930:CDS |
| TGGTGCAACTTACAAATCAA+TGG | 0.554753 | 1.4:+30362445 | MS.gene064930:CDS |
| CTTCCTGTCCCTGGATCTGG+TGG | 0.555351 | 1.4:+30367518 | MS.gene064930:CDS |
| TGCTTTGGCAAGAGAGTGCA+AGG | 0.558334 | 1.4:+30366851 | MS.gene064930:CDS |
| GTTGAAGCTGGAGGAAAGAA+TGG | 0.560027 | 1.4:+30362646 | MS.gene064930:CDS |
| ATTACAAATCTCAAAGTTGG+AGG | 0.561995 | 1.4:-30362261 | None:intergenic |
| GGAGTTTGGTCAGGATGCTA+AGG | 0.562090 | 1.4:+30359749 | MS.gene064930:CDS |
| GCACGGTTATCCGGTGAATG+AGG | 0.562486 | 1.4:-30366831 | None:intergenic |
| TCTTGTCCCTGCAACACAGA+AGG | 0.562826 | 1.4:+30367357 | MS.gene064930:CDS |
| TGTCTCAAAATTGGAGCCAC+TGG | 0.563417 | 1.4:+30366703 | MS.gene064930:CDS |
| TTGCCTGAGGTTCATTCATG+CGG | 0.565671 | 1.4:-30367637 | None:intergenic |
| AGTATACATAGGATCTTGTA+CGG | 0.567622 | 1.4:+30366890 | MS.gene064930:CDS |
| ATTGGTGAAATAAGTGTGGC+TGG | 0.569430 | 1.4:+30362425 | MS.gene064930:CDS |
| CATTTGGTCAATTTGCAACA+GGG | 0.572263 | 1.4:+30361167 | MS.gene064930:CDS |
| CACATCGCTCTTGCTCAGGA+AGG | 0.575169 | 1.4:+30360993 | MS.gene064930:CDS |
| AAAATTGACCGAGTATACAT+AGG | 0.575575 | 1.4:+30366879 | MS.gene064930:CDS |
| AGTACTTGATATCTTGCTCA+TGG | 0.576727 | 1.4:-30360810 | None:intergenic |
| CATTACATATAAATATCTTG+AGG | 0.587292 | 1.4:+30362687 | MS.gene064930:CDS |
| AGGTCATTGCAGGCCAGGAG+AGG | 0.593257 | 1.4:+30361013 | MS.gene064930:CDS |
| TACAAGATCCTATGTATACT+CGG | 0.598789 | 1.4:-30366887 | None:intergenic |
| TTACCACCAGATCCAGGGAC+AGG | 0.600757 | 1.4:-30367521 | None:intergenic |
| CCCAAACAAGCACCACAGCT+AGG | 0.602329 | 1.4:-30367593 | None:intergenic |
| AATGGCGAAAGCTTCTGAGA+AGG | 0.607979 | 1.4:+30359632 | MS.gene064930:CDS |
| TCAACCCAAATATTATCCCC+AGG | 0.614366 | 1.4:-30359666 | None:intergenic |
| TACATAGGATCTTGTACGGG+TGG | 0.615320 | 1.4:+30366894 | MS.gene064930:CDS |
| GGTTGACGCTGTGTCTGTTG+AGG | 0.615350 | 1.4:-30359470 | None:intergenic |
| TGGCGAAAGCTTCTGAGAAG+GGG | 0.629318 | 1.4:+30359634 | MS.gene064930:CDS |
| AGAAGGGGCATGTTACACCT+GGG | 0.630209 | 1.4:+30359649 | MS.gene064930:CDS |
| CAGGTAAATCCAGATTACAA+AGG | 0.639525 | 1.4:+30360963 | MS.gene064930:intron |
| AACCTCCTTCTTCCAGACCA+AGG | 0.640258 | 1.4:+30359264 | MS.gene064930:CDS |
| AAGTCTTACCACCAGATCCA+GGG | 0.642020 | 1.4:-30367526 | None:intergenic |
| TGTTGCAGGGACAAGAAATG+TGG | 0.650698 | 1.4:-30367350 | None:intergenic |
| GGACTAACCTTGGCAACCAG+TGG | 0.655420 | 1.4:-30366719 | None:intergenic |
| GTATACATAGGATCTTGTAC+GGG | 0.656739 | 1.4:+30366891 | MS.gene064930:CDS |
| AAGATGCTAGGAAAGAAGCA+GGG | 0.660307 | 1.4:-30367663 | None:intergenic |
| TTATATGTAATGCTATCTGG+AGG | 0.666348 | 1.4:-30362676 | None:intergenic |
| CTTCCTGAGCAAGAGCGATG+TGG | 0.677611 | 1.4:-30360992 | None:intergenic |
| GAAGGGGCATGTTACACCTG+GGG | 0.687521 | 1.4:+30359650 | MS.gene064930:CDS |
| ACGGATGACATTGTGCAACA+TGG | 0.689455 | 1.4:+30362621 | MS.gene064930:CDS |
| TACCTTGGTCTGGAAGAAGG+AGG | 0.694918 | 1.4:-30359266 | None:intergenic |
| ACACCTGCTAGTCCTAGCTG+TGG | 0.700530 | 1.4:+30367581 | MS.gene064930:CDS |
| GTTGCAGGGACAAGAAATGT+GGG | 0.707328 | 1.4:-30367349 | None:intergenic |
| AGAAGCAGGGAACTTGCCTG+AGG | 0.716781 | 1.4:-30367650 | None:intergenic |
| GATTATTGGTGAAATAAGTG+TGG | 0.724786 | 1.4:+30362421 | MS.gene064930:intron |
| AACATGGTTGTTGAAGCTGG+AGG | 0.764525 | 1.4:+30362637 | MS.gene064930:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 30359241 | 30367679 | 30359241 | ID=MS.gene064930 |
| chr1.4 | mRNA | 30359241 | 30367679 | 30359241 | ID=MS.gene064930.t1;Parent=MS.gene064930 |
| chr1.4 | exon | 30359241 | 30359285 | 30359241 | ID=MS.gene064930.t1.exon1;Parent=MS.gene064930.t1 |
| chr1.4 | CDS | 30359241 | 30359285 | 30359241 | ID=cds.MS.gene064930.t1;Parent=MS.gene064930.t1 |
| chr1.4 | exon | 30359380 | 30359508 | 30359380 | ID=MS.gene064930.t1.exon2;Parent=MS.gene064930.t1 |
| chr1.4 | CDS | 30359380 | 30359508 | 30359380 | ID=cds.MS.gene064930.t1;Parent=MS.gene064930.t1 |
| chr1.4 | exon | 30359600 | 30359770 | 30359600 | ID=MS.gene064930.t1.exon3;Parent=MS.gene064930.t1 |
| chr1.4 | CDS | 30359600 | 30359770 | 30359600 | ID=cds.MS.gene064930.t1;Parent=MS.gene064930.t1 |
| chr1.4 | exon | 30360712 | 30360864 | 30360712 | ID=MS.gene064930.t1.exon4;Parent=MS.gene064930.t1 |
| chr1.4 | CDS | 30360712 | 30360864 | 30360712 | ID=cds.MS.gene064930.t1;Parent=MS.gene064930.t1 |
| chr1.4 | exon | 30360966 | 30361034 | 30360966 | ID=MS.gene064930.t1.exon5;Parent=MS.gene064930.t1 |
| chr1.4 | CDS | 30360966 | 30361034 | 30360966 | ID=cds.MS.gene064930.t1;Parent=MS.gene064930.t1 |
| chr1.4 | exon | 30361127 | 30361243 | 30361127 | ID=MS.gene064930.t1.exon6;Parent=MS.gene064930.t1 |
| chr1.4 | CDS | 30361127 | 30361243 | 30361127 | ID=cds.MS.gene064930.t1;Parent=MS.gene064930.t1 |
| chr1.4 | exon | 30362258 | 30362332 | 30362258 | ID=MS.gene064930.t1.exon7;Parent=MS.gene064930.t1 |
| chr1.4 | CDS | 30362258 | 30362332 | 30362258 | ID=cds.MS.gene064930.t1;Parent=MS.gene064930.t1 |
| chr1.4 | exon | 30362422 | 30362499 | 30362422 | ID=MS.gene064930.t1.exon8;Parent=MS.gene064930.t1 |
| chr1.4 | CDS | 30362422 | 30362499 | 30362422 | ID=cds.MS.gene064930.t1;Parent=MS.gene064930.t1 |
| chr1.4 | exon | 30362613 | 30362708 | 30362613 | ID=MS.gene064930.t1.exon9;Parent=MS.gene064930.t1 |
| chr1.4 | CDS | 30362613 | 30362708 | 30362613 | ID=cds.MS.gene064930.t1;Parent=MS.gene064930.t1 |
| chr1.4 | exon | 30366108 | 30366157 | 30366108 | ID=MS.gene064930.t1.exon10;Parent=MS.gene064930.t1 |
| chr1.4 | CDS | 30366108 | 30366157 | 30366108 | ID=cds.MS.gene064930.t1;Parent=MS.gene064930.t1 |
| chr1.4 | exon | 30366679 | 30366733 | 30366679 | ID=MS.gene064930.t1.exon11;Parent=MS.gene064930.t1 |
| chr1.4 | CDS | 30366679 | 30366733 | 30366679 | ID=cds.MS.gene064930.t1;Parent=MS.gene064930.t1 |
| chr1.4 | exon | 30366831 | 30366962 | 30366831 | ID=MS.gene064930.t1.exon12;Parent=MS.gene064930.t1 |
| chr1.4 | CDS | 30366831 | 30366962 | 30366831 | ID=cds.MS.gene064930.t1;Parent=MS.gene064930.t1 |
| chr1.4 | exon | 30367331 | 30367378 | 30367331 | ID=MS.gene064930.t1.exon13;Parent=MS.gene064930.t1 |
| chr1.4 | CDS | 30367331 | 30367378 | 30367331 | ID=cds.MS.gene064930.t1;Parent=MS.gene064930.t1 |
| chr1.4 | exon | 30367497 | 30367679 | 30367497 | ID=MS.gene064930.t1.exon14;Parent=MS.gene064930.t1 |
| chr1.4 | CDS | 30367497 | 30367679 | 30367497 | ID=cds.MS.gene064930.t1;Parent=MS.gene064930.t1 |
| Gene Sequence |
| Protein sequence |
