AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene011362

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011362.t1 MTR_4g104380 98.638 514 7 0 1 514 1 514 0.0 1040
MS.gene011362.t1 MTR_3g075400 74.409 508 130 0 7 514 2 509 0.0 785
MS.gene011362.t1 MTR_4g007500 78.894 398 31 4 30 427 20 364 0.0 611
MS.gene011362.t1 MTR_6g027280 52.353 170 35 5 48 199 95 236 5.55e-36 134
MS.gene011362.t1 MTR_8g092620 27.397 292 200 6 226 513 14 297 2.09e-31 123
MS.gene011362.t1 MTR_4g119620 29.804 255 167 6 228 475 76 325 1.44e-29 119
MS.gene011362.t1 MTR_4g119620 26.792 265 175 7 263 513 18 277 4.50e-19 88.6
MS.gene011362.t1 MTR_8g092620 28.571 273 183 6 226 494 21 285 2.11e-29 118
MS.gene011362.t1 MTR_7g086360 27.206 272 193 2 226 492 51 322 1.24e-27 114
MS.gene011362.t1 MTR_7g086360 27.143 210 138 6 310 513 51 251 6.30e-15 76.6
MS.gene011362.t1 MTR_4g102520 25.962 312 206 8 218 513 282 584 3.31e-24 106
MS.gene011362.t1 MTR_4g102520 33.019 106 65 3 289 392 273 374 7.67e-12 68.2
MS.gene011362.t1 MTR_8g106790 26.948 308 176 9 241 513 27 320 2.56e-22 98.2
MS.gene011362.t1 MTR_8g106790 25.818 275 172 8 225 475 58 324 7.80e-17 82.0
MS.gene011362.t1 MTR_8g106790 27.027 222 140 7 306 513 7 220 1.87e-13 72.0
MS.gene011362.t1 MTR_1g072140 26.073 303 214 6 219 513 12 312 3.80e-22 97.4
MS.gene011362.t1 MTR_8g092620 28.837 215 143 5 226 436 14 222 6.03e-22 95.1
MS.gene011362.t1 MTR_2g012630 26.906 223 157 3 245 466 31 248 1.94e-20 95.9
MS.gene011362.t1 MTR_2g012630 26.974 152 109 2 228 378 98 248 3.40e-15 79.3
MS.gene011362.t1 MTR_2g012630 23.684 228 164 4 285 509 29 249 1.77e-14 77.0
MS.gene011362.t1 MTR_2g012630 26.906 223 157 3 245 466 31 248 2.02e-20 95.9
MS.gene011362.t1 MTR_2g012630 26.974 152 109 2 228 378 98 248 3.03e-15 79.3
MS.gene011362.t1 MTR_2g012630 23.684 228 164 4 285 509 29 249 1.97e-14 76.6
MS.gene011362.t1 MTR_6g004040 27.049 244 168 5 224 462 7 245 2.13e-20 95.5
MS.gene011362.t1 MTR_6g004040 24.887 221 156 4 284 501 29 242 4.40e-16 82.0
MS.gene011362.t1 MTR_6g004040 24.103 195 146 2 221 414 50 243 1.39e-12 70.9
MS.gene011362.t1 MTR_6g004040 21.154 208 153 4 310 513 12 212 2.78e-11 66.6
MS.gene011362.t1 MTR_1g072740 27.444 266 167 8 218 468 281 535 4.75e-18 87.8
MS.gene011362.t1 MTR_1g072740 33.019 106 65 3 289 392 272 373 5.64e-12 68.6
MS.gene011362.t1 MTR_4g105700 26.394 269 175 8 263 514 350 612 1.50e-17 86.7
MS.gene011362.t1 MTR_4g105700 31.351 185 116 5 253 427 41 224 2.96e-16 82.4
MS.gene011362.t1 MTR_8g104010 25.322 233 163 4 283 513 600 823 5.66e-17 84.7
MS.gene011362.t1 MTR_8g104010 26.119 268 163 13 226 471 669 923 5.95e-13 72.0
MS.gene011362.t1 MTR_8g104010 25.322 233 163 4 283 513 517 740 6.03e-17 84.7
MS.gene011362.t1 MTR_8g104010 26.119 268 163 13 226 471 586 840 5.36e-13 72.0
MS.gene011362.t1 MTR_8g104010 25.322 233 163 4 283 513 880 1103 9.40e-17 84.3
MS.gene011362.t1 MTR_8g104010 26.119 268 163 13 226 471 949 1203 6.30e-13 72.0
MS.gene011362.t1 MTR_1g097140 24.912 285 199 7 225 504 118 392 9.76e-17 82.8
MS.gene011362.t1 MTR_1g097140 24.306 288 155 8 224 498 74 311 5.83e-11 64.7
MS.gene011362.t1 MTR_2g436510 22.222 261 185 7 224 477 163 412 3.78e-16 81.3
MS.gene011362.t1 MTR_3g045130 22.892 249 180 3 267 514 274 511 4.23e-16 81.3
MS.gene011362.t1 MTR_3g045130 23.320 253 184 5 232 482 277 521 4.49e-12 68.9
MS.gene011362.t1 MTR_8g038550 22.635 296 181 5 221 514 216 465 1.80e-15 79.7
MS.gene011362.t1 MTR_3g106080 27.815 302 188 11 218 513 337 614 5.43e-15 78.2
MS.gene011362.t1 MTR_3g106080 21.951 205 158 2 226 429 415 618 1.21e-11 67.8
MS.gene011362.t1 MTR_1g054300 27.660 141 101 1 202 342 342 481 1.33e-14 76.6
MS.gene011362.t1 MTR_1g054300 25.630 238 146 7 245 471 264 481 1.52e-11 67.0
MS.gene011362.t1 MTR_4g095058 23.000 300 172 6 223 512 321 571 2.25e-14 76.3
MS.gene011362.t1 MTR_4g095058 23.000 300 172 6 223 512 323 573 2.40e-14 75.9
MS.gene011362.t1 MTR_2g019400 27.011 174 114 3 316 489 2 162 9.45e-14 72.0
MS.gene011362.t1 MTR_3g449990 24.522 314 209 11 215 513 255 555 1.10e-13 73.9
MS.gene011362.t1 MTR_5g016350 23.381 278 195 6 245 512 245 514 3.58e-13 72.0
MS.gene011362.t1 MTR_5g016350 23.381 278 195 6 245 512 309 578 4.75e-13 72.0
MS.gene011362.t1 MTR_5g016370 24.172 302 166 7 223 512 340 590 1.03e-12 70.9
MS.gene011362.t1 MTR_2g016690 23.693 287 193 8 245 514 23 300 1.92e-12 68.9
MS.gene011362.t1 MTR_1g034785 23.113 212 141 3 240 429 86 297 2.57e-12 69.3
MS.gene011362.t1 MTR_4g125710 24.812 266 169 8 245 489 33 288 7.91e-12 67.0
MS.gene011362.t1 MTR_3g052220 22.422 223 144 7 280 482 222 435 8.54e-12 67.8
MS.gene011362.t1 MTR_3g052220 27.108 166 107 4 328 483 227 388 1.18e-11 67.4
MS.gene011362.t1 MTR_7g076230 25.243 206 146 4 281 483 378 578 1.13e-11 67.8
MS.gene011362.t1 MTR_6g005220 23.741 278 172 11 274 513 110 385 1.22e-11 67.4
MS.gene011362.t1 MTR_2g029940 23.377 231 162 5 245 473 225 442 1.45e-11 67.0
MS.gene011362.t1 MTR_2g034500 24.188 277 161 9 225 471 236 493 1.59e-11 67.8
MS.gene011362.t1 MTR_4g071130 26.394 269 166 12 225 485 46 290 2.79e-11 67.0
MS.gene011362.t1 MTR_1g044720 25.000 204 147 4 232 431 224 425 4.74e-11 65.5
MS.gene011362.t1 MTR_1g069345 24.127 315 207 14 217 513 203 503 7.65e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011362.t1 AT5G50230 71.203 507 146 0 8 514 3 509 0.0 784
MS.gene011362.t1 AT5G64730 26.490 302 216 4 213 513 1 297 4.69e-30 119
MS.gene011362.t1 AT3G49660 28.682 258 168 5 226 475 67 316 1.37e-29 119
MS.gene011362.t1 AT3G49660 25.581 301 205 6 226 514 20 313 1.37e-27 113
MS.gene011362.t1 AT2G43770 26.838 272 194 2 226 492 49 320 2.00e-28 116
MS.gene011362.t1 AT2G43770 26.923 208 141 5 310 513 49 249 4.66e-15 77.0
MS.gene011362.t1 AT1G48630 26.403 303 185 8 241 513 27 321 2.64e-22 98.2
MS.gene011362.t1 AT1G48630 26.087 276 171 8 225 475 58 325 1.48e-17 84.3
MS.gene011362.t1 AT1G48630 25.455 220 146 7 306 513 7 220 1.43e-12 69.3
MS.gene011362.t1 AT3G18130 26.733 303 184 8 241 513 27 321 3.08e-22 98.2
MS.gene011362.t1 AT3G18130 26.087 276 171 8 225 475 58 325 1.67e-17 84.0
MS.gene011362.t1 AT3G18130 25.000 220 147 7 306 513 7 220 1.11e-11 66.6
MS.gene011362.t1 AT1G10580 24.038 312 212 8 218 513 270 572 3.46e-21 97.4
MS.gene011362.t1 AT3G16650 25.191 262 175 8 224 477 163 411 7.56e-21 95.9
MS.gene011362.t1 AT5G23430 28.111 217 150 4 224 435 7 222 1.16e-20 96.3
MS.gene011362.t1 AT5G23430 25.229 218 153 4 285 499 30 240 1.80e-15 80.1
MS.gene011362.t1 AT5G23430 24.103 195 146 2 221 414 50 243 9.76e-12 68.2
MS.gene011362.t1 AT5G23430 28.111 217 150 4 224 435 7 222 1.16e-20 96.3
MS.gene011362.t1 AT5G23430 25.229 218 153 4 285 499 30 240 1.80e-15 80.1
MS.gene011362.t1 AT5G23430 24.103 195 146 2 221 414 50 243 9.76e-12 68.2
MS.gene011362.t1 AT5G23430 28.111 217 150 4 224 435 7 222 1.21e-20 96.3
MS.gene011362.t1 AT5G23430 25.229 218 153 4 285 499 30 240 1.85e-15 80.1
MS.gene011362.t1 AT5G23430 24.103 195 146 2 221 414 50 243 1.01e-11 68.2
MS.gene011362.t1 AT1G11160 27.165 254 174 6 224 471 6 254 1.25e-20 96.3
MS.gene011362.t1 AT1G11160 23.348 227 168 3 284 509 28 249 6.98e-13 72.0
MS.gene011362.t1 AT1G11160 27.165 254 174 6 224 471 6 254 1.33e-20 96.3
MS.gene011362.t1 AT1G11160 23.348 227 168 3 284 509 28 249 7.36e-13 72.0
MS.gene011362.t1 AT1G18080 25.484 310 180 9 241 513 27 322 1.66e-20 93.2
MS.gene011362.t1 AT1G18080 28.440 218 143 6 306 513 7 221 5.08e-15 76.6
MS.gene011362.t1 AT1G18080 23.827 277 177 8 225 475 58 326 2.42e-14 74.7
MS.gene011362.t1 AT1G11160 27.165 254 174 6 224 471 6 254 1.76e-20 95.9
MS.gene011362.t1 AT1G11160 23.348 227 168 3 284 509 28 249 9.48e-13 71.6
MS.gene011362.t1 AT5G08390 28.571 217 149 4 224 435 7 222 2.82e-20 95.1
MS.gene011362.t1 AT5G08390 24.074 216 158 3 285 499 30 240 6.66e-15 78.2
MS.gene011362.t1 AT5G08390 22.564 195 149 2 221 414 50 243 4.66e-11 65.9
MS.gene011362.t1 AT5G08390 25.974 154 104 3 226 374 97 245 6.89e-11 65.5
MS.gene011362.t1 AT4G15900 23.970 267 173 9 224 477 169 418 3.12e-19 90.9
MS.gene011362.t1 AT4G02730 25.704 284 202 6 214 490 23 304 3.57e-19 89.4
MS.gene011362.t1 AT4G02730 21.654 254 186 7 267 513 38 285 1.76e-13 72.0
MS.gene011362.t1 AT1G04510 26.509 381 234 13 58 431 87 428 1.56e-18 89.0
MS.gene011362.t1 AT1G04510 26.509 381 234 13 58 431 87 428 1.56e-18 89.0
MS.gene011362.t1 AT1G61210 26.378 254 176 6 224 472 6 253 5.29e-18 88.2
MS.gene011362.t1 AT1G61210 24.324 222 156 5 284 501 28 241 1.41e-12 70.9
MS.gene011362.t1 AT5G13480 23.469 294 179 4 221 513 167 415 6.06e-18 87.4
MS.gene011362.t1 AT5G13480 23.469 294 179 4 221 513 144 392 6.30e-18 87.4
MS.gene011362.t1 AT5G13480 23.469 294 179 4 221 513 144 392 8.87e-18 87.0
MS.gene011362.t1 AT1G61210 26.872 227 160 4 224 445 6 231 9.14e-18 87.4
MS.gene011362.t1 AT1G61210 24.324 222 156 5 284 501 28 241 2.60e-12 70.1
MS.gene011362.t1 AT1G61210 25.157 159 116 3 226 383 96 252 1.03e-11 68.2
MS.gene011362.t1 AT5G13480 23.469 294 179 4 221 513 156 404 9.70e-18 87.0
MS.gene011362.t1 AT1G61210 26.872 227 160 4 224 445 6 231 9.90e-18 87.4
MS.gene011362.t1 AT1G61210 24.324 222 156 5 284 501 28 241 2.67e-12 70.1
MS.gene011362.t1 AT1G61210 25.157 159 116 3 226 383 96 252 1.16e-11 68.2
MS.gene011362.t1 AT5G52820 22.849 337 214 11 219 513 97 429 1.89e-17 85.5
MS.gene011362.t1 AT5G52820 26.816 179 111 3 164 342 313 471 1.87e-14 76.3
MS.gene011362.t1 AT2G33340 23.758 463 294 16 58 507 87 503 2.32e-16 82.4
MS.gene011362.t1 AT2G33340 23.758 463 294 16 58 507 87 503 2.32e-16 82.4
MS.gene011362.t1 AT2G33340 24.011 379 243 11 58 430 87 426 4.25e-16 81.3
MS.gene011362.t1 AT5G16750 26.038 265 177 6 264 513 350 610 4.52e-16 82.0
MS.gene011362.t1 AT5G16750 26.512 215 149 5 254 461 42 254 4.55e-15 79.0
MS.gene011362.t1 AT5G16750 23.443 273 175 8 221 471 392 652 2.35e-11 67.0
MS.gene011362.t1 AT3G27640 24.911 281 169 8 274 513 130 409 1.34e-15 80.1
MS.gene011362.t1 AT3G27640 24.911 281 169 8 274 513 111 390 1.42e-15 79.7
MS.gene011362.t1 AT5G43920 25.806 310 208 11 218 513 212 513 2.10e-15 79.3
MS.gene011362.t1 AT1G11160 26.087 207 146 4 267 471 11 212 3.02e-15 79.3
MS.gene011362.t1 AT1G11160 28.387 155 107 3 226 378 54 206 3.89e-14 75.9
MS.gene011362.t1 AT1G11160 25.123 203 146 3 308 509 10 207 1.85e-12 70.5
MS.gene011362.t1 AT1G49040 26.937 271 171 12 241 498 866 1122 3.14e-15 79.3
MS.gene011362.t1 AT1G49040 24.481 241 155 7 283 513 866 1089 6.07e-14 75.5
MS.gene011362.t1 AT1G49040 24.862 181 125 6 233 409 944 1117 1.37e-12 71.2
MS.gene011362.t1 AT3G18140 24.373 279 197 7 245 514 8 281 1.00e-14 75.5
MS.gene011362.t1 AT3G18140 25.000 184 122 5 235 406 124 303 7.85e-11 63.5
MS.gene011362.t1 AT5G67320 23.264 288 162 5 226 503 361 599 1.19e-14 77.0
MS.gene011362.t1 AT4G03020 26.104 249 164 6 275 513 232 470 1.46e-14 76.6
MS.gene011362.t1 AT4G03020 26.104 249 164 6 275 513 232 470 1.46e-14 76.6
MS.gene011362.t1 AT4G03020 26.104 249 164 6 275 513 232 470 1.46e-14 76.6
MS.gene011362.t1 AT3G49180 24.645 211 142 3 243 436 91 301 7.22e-14 73.9
MS.gene011362.t1 AT5G08560 23.944 284 164 10 278 513 280 559 1.01e-13 74.3
MS.gene011362.t1 AT5G08560 23.944 284 164 10 278 513 280 559 1.01e-13 74.3
MS.gene011362.t1 AT5G08560 23.944 284 164 10 278 513 280 559 1.01e-13 74.3
MS.gene011362.t1 AT5G25150 26.367 311 177 11 218 513 340 613 1.59e-13 73.6
MS.gene011362.t1 AT5G25150 22.439 205 157 2 226 429 414 617 6.10e-11 65.5
MS.gene011362.t1 AT5G25150 26.367 311 177 11 218 513 340 613 1.61e-13 73.6
MS.gene011362.t1 AT5G25150 22.439 205 157 2 226 429 414 617 6.57e-11 65.5
MS.gene011362.t1 AT2G47410 28.249 177 109 3 225 383 49 225 1.93e-13 73.9
MS.gene011362.t1 AT2G47410 28.249 177 109 3 225 383 231 407 1.96e-13 73.9
MS.gene011362.t1 AT2G47410 28.249 177 109 3 225 383 230 406 2.10e-13 73.9
MS.gene011362.t1 AT2G21390 24.000 275 172 7 225 468 46 314 7.86e-13 72.0
MS.gene011362.t1 AT2G47410 26.768 198 127 3 225 420 49 230 1.09e-12 71.6
MS.gene011362.t1 AT2G47410 26.768 198 127 3 225 420 49 230 1.09e-12 71.6
MS.gene011362.t1 AT2G16405 23.308 266 182 7 268 513 212 475 1.17e-12 70.5
MS.gene011362.t1 AT2G41500 23.140 242 174 6 277 514 303 536 1.25e-12 70.5
MS.gene011362.t1 AT2G41500 23.320 253 182 6 226 474 294 538 1.73e-12 70.1
MS.gene011362.t1 AT2G41500 23.140 242 174 6 277 514 303 536 1.25e-12 70.5
MS.gene011362.t1 AT2G41500 23.320 253 182 6 226 474 294 538 1.73e-12 70.1
MS.gene011362.t1 AT2G47410 26.768 198 127 3 225 420 231 412 1.26e-12 71.2
MS.gene011362.t1 AT4G29830 23.810 294 195 7 227 498 11 297 1.32e-12 69.3
MS.gene011362.t1 AT2G22040 23.693 287 191 9 245 514 13 288 1.75e-12 68.9
MS.gene011362.t1 AT2G22040 25.134 187 125 4 235 409 130 313 1.90e-12 68.6
MS.gene011362.t1 AT1G71840 23.841 302 208 10 224 513 64 355 2.68e-12 68.9
MS.gene011362.t1 AT3G15610 23.810 273 195 7 245 513 34 297 3.81e-12 68.2
MS.gene011362.t1 AT1G73720 24.848 165 111 3 328 483 226 386 7.74e-12 68.2
MS.gene011362.t1 AT1G73720 22.791 215 152 6 280 482 221 433 1.59e-11 67.0
MS.gene011362.t1 AT5G49430 26.570 207 128 4 225 429 240 424 3.68e-11 66.6
MS.gene011362.t1 AT5G49430 26.570 207 128 4 225 429 240 424 3.68e-11 66.6
MS.gene011362.t1 AT1G62020 22.909 275 175 7 225 468 46 314 4.56e-11 66.2
MS.gene011362.t1 AT2G26490 26.820 261 157 13 244 491 146 385 7.01e-11 64.7
MS.gene011362.t1 AT1G52730 22.993 274 196 8 245 513 34 297 8.57e-11 63.9
MS.gene011362.t1 AT1G52730 22.993 274 196 8 245 513 34 297 8.57e-11 63.9
MS.gene011362.t1 AT5G14530 22.727 308 206 10 200 495 8 295 8.71e-11 63.9

Find 105 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAACAGAGTGGCTTCTAAT+TGG 0.280252 8.4:+21594269 MS.gene011362:CDS
TCCTTAAGTTCAAGTCTTAC+TGG 0.289367 8.4:+21593737 MS.gene011362:CDS
GATTGTATTATACCTCATTT+AGG 0.316444 8.4:-21592627 None:intergenic
ACGACCGTACTATAAAAGTT+TGG 0.316749 8.4:+21593954 MS.gene011362:CDS
CGAGTCCGTCATACCCTTAC+TGG 0.338442 8.4:+21593863 MS.gene011362:CDS
CTCTCAATTAAAGACAGATT+TGG 0.339284 8.4:+21592450 MS.gene011362:CDS
TCAATCGCAACTTGTTCTTT+CGG 0.343457 8.4:-21591339 None:intergenic
TTGTTTCAATCTGGTCATAA+TGG 0.359886 8.4:+21594476 MS.gene011362:CDS
CTTCTGTTCTCTGTTGTAGC+TGG 0.366870 8.4:+21594410 MS.gene011362:CDS
GCAAAGAGCATTGCAGTTGC+TGG 0.371762 8.4:-21594017 None:intergenic
TCAATATCCCTTTCTCGAAA+TGG 0.373411 8.4:+21594157 MS.gene011362:CDS
ACAACAACAATGGTGAAAAC+TGG 0.379287 8.4:+21591312 None:intergenic
TCGATGGTAATCTTCGGTTA+TGG 0.380769 8.4:+21594077 MS.gene011362:CDS
TTCAGAACTAAAAGCACAAC+TGG 0.383721 8.4:+21592513 MS.gene011362:CDS
ATCGACATGTCCAGAAAATA+TGG 0.387589 8.4:-21594059 None:intergenic
CGACCGTACTATAAAAGTTT+GGG 0.390463 8.4:+21593955 MS.gene011362:CDS
TGGCTGCCTTGGCTCAGTAT+TGG 0.392787 8.4:+21593757 MS.gene011362:CDS
AATTCTAGTAGATTGATTAC+TGG 0.396540 8.4:+21593671 MS.gene011362:CDS
GAAAGAGAAAGCAGAGAACT+TGG 0.396574 8.4:+21591970 MS.gene011362:CDS
TCAAGTCTTACTGGCTGCCT+TGG 0.397402 8.4:+21593746 MS.gene011362:CDS
TTCTAGACCCATCGTTTCTC+AGG 0.404660 8.4:+21591443 MS.gene011362:CDS
AGAATTGTACTCGAACAATA+AGG 0.407571 8.4:-21593654 None:intergenic
CAATATTTCCATTTCGAGAA+AGG 0.408563 8.4:-21594165 None:intergenic
CTGATGGATCTGTGCATATA+TGG 0.411583 8.4:+21594341 MS.gene011362:CDS
GGAATGGAATTGTTTCAATC+TGG 0.416661 8.4:+21594467 MS.gene011362:CDS
CGATGGTAATCTTCGGTTAT+GGG 0.416788 8.4:+21594078 MS.gene011362:CDS
AAGACATGGTTCAGCGGCTA+AGG 0.439770 8.4:+21593489 MS.gene011362:CDS
ATTCCTGTCAGCTGAAGCTA+GGG 0.443091 8.4:-21594449 None:intergenic
AATATTTCCATTTCGAGAAA+GGG 0.445723 8.4:-21594164 None:intergenic
CTTTATCCGTGTGTCCAGTA+AGG 0.447371 8.4:-21593877 None:intergenic
TTTGTTTGATGTGCGATCTC+TGG 0.451133 8.4:+21594219 MS.gene011362:CDS
GCCAGTAAGACTTGAACTTA+AGG 0.454568 8.4:-21593738 None:intergenic
TCCCTTCCAATTGCAAATAC+AGG 0.460830 8.4:+21593603 MS.gene011362:CDS
CATTCCTGTCAGCTGAAGCT+AGG 0.467857 8.4:-21594450 None:intergenic
AATAACAGATCGATTATCAT+TGG 0.473031 8.4:-21593792 None:intergenic
GCTCTTTGCTTCAGCACAGA+TGG 0.474209 8.4:+21594031 MS.gene011362:CDS
CTAGAAAGTGCGAGAAAAGC+AGG 0.474966 8.4:-21591426 None:intergenic
GACAACAATCATGTTGCCGC+TGG 0.477287 8.4:+21594313 MS.gene011362:CDS
ATTTAGCCTGTATTTGCAAT+TGG 0.481713 8.4:-21593609 None:intergenic
GAAAGCAGAGAACTTGGAAG+TGG 0.488324 8.4:+21591976 MS.gene011362:CDS
AACCCCTAGCTTCAGCTGAC+AGG 0.489230 8.4:+21594446 MS.gene011362:CDS
ATTCTAGTAGATTGATTACT+GGG 0.491572 8.4:+21593672 MS.gene011362:CDS
GCGGCAACATGATTGTTGTC+TGG 0.492362 8.4:-21594310 None:intergenic
TGTTGTAGCTGGAGTGGAAT+GGG 0.499332 8.4:+21594421 MS.gene011362:CDS
AGCCTGTATTTGCAATTGGA+AGG 0.502314 8.4:-21593605 None:intergenic
AAACGCCATCTTCTCGAAGA+AGG 0.507490 8.4:+21591393 MS.gene011362:CDS
GCCTGTATTTGCAATTGGAA+GGG 0.520159 8.4:-21593604 None:intergenic
CATATATGGTCAATATCTAA+AGG 0.520412 8.4:+21594355 MS.gene011362:CDS
GAGCGCCTTCTTCGAGAAGA+TGG 0.521377 8.4:-21591398 None:intergenic
GACATGTCGATGGTAATCTT+CGG 0.525301 8.4:+21594071 MS.gene011362:CDS
AAAGTTTGGGACTTGATGAA+AGG 0.527945 8.4:+21593968 MS.gene011362:CDS
TACTTCCACAACAAGTTGCT+CGG 0.529307 8.4:-21592035 None:intergenic
CAAAGAGCATTGCAGTTGCT+GGG 0.531625 8.4:-21594016 None:intergenic
GTTGCCGCTGGGTCTACTGA+TGG 0.534764 8.4:+21594325 MS.gene011362:CDS
GTGCAAATTATCCCTTCCAC+TGG 0.536187 8.4:-21594194 None:intergenic
GTTCTCTGTTGTAGCTGGAG+TGG 0.537003 8.4:+21594415 MS.gene011362:CDS
AAAGTGTGGGATACAAATAC+AGG 0.539323 8.4:+21593713 MS.gene011362:CDS
ATTGTTCGTCGAAGTGAAGA+AGG 0.542555 8.4:+21593554 MS.gene011362:CDS
TATGTGTGGGATCTTAGCTC+AGG 0.543218 8.4:+21593839 MS.gene011362:CDS
CTAGCTTCAGCTGACAGGAA+TGG 0.547140 8.4:+21594451 MS.gene011362:CDS
AGAAGGAGTTGACAGAATTG+AGG 0.554456 8.4:+21592119 MS.gene011362:CDS
GTGCGATCTCTGGAAGTTTG+TGG 0.559305 8.4:+21594229 MS.gene011362:CDS
TGCAATTTCTGATATGCAGA+AGG 0.560064 8.4:+21592102 MS.gene011362:CDS
CTTGATGTTAGAAAAGATGA+AGG 0.562481 8.4:+21592594 MS.gene011362:CDS
TGAAATAATCAGTACTCTGA+AGG 0.562507 8.4:+21594378 MS.gene011362:CDS
GTTATCCGAGCAACTTGTTG+TGG 0.566280 8.4:+21592030 MS.gene011362:CDS
CTCAAACAACTTGTATGTGT+GGG 0.569983 8.4:+21593826 MS.gene011362:CDS
GCTCAAACAACTTGTATGTG+TGG 0.578898 8.4:+21593825 MS.gene011362:CDS
CTGTTGTAGCTGGAGTGGAA+TGG 0.580319 8.4:+21594420 MS.gene011362:CDS
AGACATGGTTCAGCGGCTAA+GGG 0.586487 8.4:+21593490 MS.gene011362:CDS
ACAACTTGCAAGGGAACAAG+TGG 0.587447 8.4:+21593526 MS.gene011362:CDS
GTTCAGCGGCTAAGGGCTAG+TGG 0.589500 8.4:+21593497 MS.gene011362:CDS
ATATTGCATTGACCAGTGGA+AGG 0.590280 8.4:+21594182 MS.gene011362:CDS
GGATGCTGAACGCCTAAATG+AGG 0.592570 8.4:+21592615 MS.gene011362:CDS
AATGCTATAAAGCTCAATCT+CGG 0.592904 8.4:+21592008 MS.gene011362:CDS
TACAGGCTAAATGCACATGA+AGG 0.593778 8.4:+21593620 MS.gene011362:CDS
ACAACAATCATGTTGCCGCT+GGG 0.594947 8.4:+21594314 MS.gene011362:CDS
GTGGTCTAGAACAACTTGCA+AGG 0.596866 8.4:+21593516 MS.gene011362:CDS
TTTATCCGTGTGTCCAGTAA+GGG 0.597962 8.4:-21593876 None:intergenic
TATGGGACATTCAGAGTGGA+AGG 0.603476 8.4:+21594095 MS.gene011362:CDS
TTGTGCGGTTGATGTTAGCA+AGG 0.604078 8.4:+21593901 MS.gene011362:CDS
AGGATCGTTCAGTTAAAGTG+TGG 0.609417 8.4:+21593699 MS.gene011362:CDS
GGATCGTTCAGTTAAAGTGT+GGG 0.613938 8.4:+21593700 MS.gene011362:CDS
CTTGCAAGGGAACAAGTGGA+TGG 0.615284 8.4:+21593530 MS.gene011362:CDS
GGAAATATTGCATTGACCAG+TGG 0.615284 8.4:+21594178 MS.gene011362:CDS
TGGTCTAGAACAACTTGCAA+GGG 0.618737 8.4:+21593517 MS.gene011362:CDS
TTCCTGTCAGCTGAAGCTAG+GGG 0.627368 8.4:-21594448 None:intergenic
TATTGCATTGACCAGTGGAA+GGG 0.632302 8.4:+21594183 MS.gene011362:CDS
TCTAGTAGATTGATTACTGG+GGG 0.632682 8.4:+21593674 MS.gene011362:CDS
GGTACGTACCTGAGAAACGA+TGG 0.636424 8.4:-21591451 None:intergenic
TGTGTCCAGTAAGGGTATGA+CGG 0.638602 8.4:-21593868 None:intergenic
TCATACCCTTACTGGACACA+CGG 0.639635 8.4:+21593871 MS.gene011362:CDS
GAGTGGAAGGCTGCTAAGTG+AGG 0.644822 8.4:+21594108 MS.gene011362:CDS
TGAGATCCAATACTGAGCCA+AGG 0.644864 8.4:-21593763 None:intergenic
AGAAAGGGATATTGACGTGA+CGG 0.645528 8.4:-21594149 None:intergenic
AGGCTAAATGCACATGAAGG+AGG 0.648402 8.4:+21593623 MS.gene011362:CDS
TATATGAAGACATGGTTCAG+CGG 0.657419 8.4:+21593483 MS.gene011362:CDS
GTACGTACCTGAGAAACGAT+GGG 0.661026 8.4:-21591450 None:intergenic
AAATGCGCTATATGAAGACA+TGG 0.661275 8.4:+21593475 MS.gene011362:CDS
TTCTAGTAGATTGATTACTG+GGG 0.672008 8.4:+21593673 MS.gene011362:CDS
CGGTTATGGGACATTCAGAG+TGG 0.682375 8.4:+21594091 MS.gene011362:CDS
ACACACGGATAAAGTTTGTG+CGG 0.689247 8.4:+21593886 MS.gene011362:CDS
AGAAGCCACTCTGTTAACTG+TGG 0.703135 8.4:-21594263 None:intergenic
ACGAGCCACAGTTAACAGAG+TGG 0.730751 8.4:+21594258 MS.gene011362:CDS
AGATCCATCAGTAGACCCAG+CGG 0.753123 8.4:-21594329 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACAATTTTCATTTTTTTTA+GGG + chr8.4:21591482-21591501 MS.gene011362:intron 10.0%
!!! TAACAATTTTCATTTTTTTT+AGG + chr8.4:21591481-21591500 MS.gene011362:intron 10.0%
!! AAAAACTCTTATAAGTTTAT+AGG - chr8.4:21592936-21592955 None:intergenic 15.0%
!! AAAGGAAACAATAAAAAAAA+TGG - chr8.4:21591542-21591561 None:intergenic 15.0%
!! AAATGAAAATTGTTAATGAA+AGG - chr8.4:21591476-21591495 None:intergenic 15.0%
!! AAATGTATTATTAGTTGAAT+CGG - chr8.4:21593440-21593459 None:intergenic 15.0%
!! AATGAAAATTGTTAATGAAA+GGG - chr8.4:21591475-21591494 None:intergenic 15.0%
!! ATAAAAAAGTTCGATTAAAC+GGG - chr8.4:21592770-21592789 None:intergenic 20.0%
!! ATGATTGAACTAATTTCTTA+CGG - chr8.4:21591574-21591593 None:intergenic 20.0%
!! GATAAAAAAGTTCGATTAAA+CGG - chr8.4:21592771-21592790 None:intergenic 20.0%
!! TTTATTGTTTCCTTTATTGT+GGG + chr8.4:21591547-21591566 MS.gene011362:intron 20.0%
!!! AATTTACCTATTTTTAACTG+TGG + chr8.4:21591790-21591809 MS.gene011362:intron 20.0%
!!! TTTTATTGTTTCCTTTATTG+TGG + chr8.4:21591546-21591565 MS.gene011362:intron 20.0%
! AAATTAATGTTGCAAAATGC+AGG + chr8.4:21592419-21592438 MS.gene011362:intron 25.0%
! AACTCATAGTGCATAATATT+TGG + chr8.4:21593151-21593170 MS.gene011362:intron 25.0%
! AACTTTCCACAGTTAAAAAT+AGG - chr8.4:21591799-21591818 None:intergenic 25.0%
! AAGTACATCAAATCAAATGT+TGG - chr8.4:21593074-21593093 None:intergenic 25.0%
! AAGTTGAAATGAATTTGCAA+TGG + chr8.4:21592809-21592828 MS.gene011362:intron 25.0%
! AATAACAGATCGATTATCAT+TGG - chr8.4:21593795-21593814 None:intergenic 25.0%
! AATATTTCCATTTCGAGAAA+GGG - chr8.4:21594167-21594186 None:intergenic 25.0%
! AATTAATGTTGCAAAATGCA+GGG + chr8.4:21592420-21592439 MS.gene011362:intron 25.0%
! AATTCTAGTAGATTGATTAC+TGG + chr8.4:21593671-21593690 MS.gene011362:CDS 25.0%
! ACTAAAAAAAATGTTGTTCG+CGG - chr8.4:21592374-21592393 None:intergenic 25.0%
! ATTCTAGTAGATTGATTACT+GGG + chr8.4:21593672-21593691 MS.gene011362:CDS 25.0%
! CATATATGGTCAATATCTAA+AGG + chr8.4:21594355-21594374 MS.gene011362:CDS 25.0%
! GATGTATTTATGTTGTTGAA+GGG + chr8.4:21591938-21591957 MS.gene011362:intron 25.0%
! GATTGTATTATACCTCATTT+AGG - chr8.4:21592630-21592649 None:intergenic 25.0%
! TAATTCAATGTGATCACATA+TGG - chr8.4:21591705-21591724 None:intergenic 25.0%
! TGATGTATTTATGTTGTTGA+AGG + chr8.4:21591937-21591956 MS.gene011362:intron 25.0%
!!! TGAAAAGAAAAAGTACTAAC+TGG - chr8.4:21593272-21593291 None:intergenic 25.0%
!!! TTTTAACTGTGGAAAGTTAT+TGG + chr8.4:21591801-21591820 MS.gene011362:intron 25.0%
AAAGATAGTTGTTTGACTGT+AGG + chr8.4:21592846-21592865 MS.gene011362:intron 30.0%
AATACATTTCCCATCTCTTT+AGG + chr8.4:21593451-21593470 MS.gene011362:intron 30.0%
AATGCTATAAAGCTCAATCT+CGG + chr8.4:21592008-21592027 MS.gene011362:CDS 30.0%
AGAATTGTACTCGAACAATA+AGG - chr8.4:21593657-21593676 None:intergenic 30.0%
AGTGTATCTTGAAACTATAG+CGG - chr8.4:21592229-21592248 None:intergenic 30.0%
ATTTAGCCTGTATTTGCAAT+TGG - chr8.4:21593612-21593631 None:intergenic 30.0%
CAAATTCTGCTATGGTATAA+CGG - chr8.4:21592200-21592219 None:intergenic 30.0%
CAATATTTCCATTTCGAGAA+AGG - chr8.4:21594168-21594187 None:intergenic 30.0%
CTCTCAATTAAAGACAGATT+TGG + chr8.4:21592450-21592469 MS.gene011362:CDS 30.0%
CTTGATGTTAGAAAAGATGA+AGG + chr8.4:21592594-21592613 MS.gene011362:CDS 30.0%
TACAAAATGTTGTCCAATAG+CGG + chr8.4:21592255-21592274 MS.gene011362:intron 30.0%
TAGTTCCAAAAGTTGTATAC+TGG - chr8.4:21592665-21592684 None:intergenic 30.0%
TCAAACAACTATCTTTGACA+AGG - chr8.4:21592842-21592861 None:intergenic 30.0%
TGAAATAATCAGTACTCTGA+AGG + chr8.4:21594378-21594397 MS.gene011362:CDS 30.0%
TTCTAGTAGATTGATTACTG+GGG + chr8.4:21593673-21593692 MS.gene011362:CDS 30.0%
! ACTACTAAGCACTATTCTTT+TGG - chr8.4:21591602-21591621 None:intergenic 30.0%
! AGAACCTTCTGAAAAAATTG+AGG - chr8.4:21592890-21592909 None:intergenic 30.0%
! AGTCCCAAACTTTTATAGTA+CGG - chr8.4:21593961-21593980 None:intergenic 30.0%
! CTATACATTTTCCTTGTGAA+TGG + chr8.4:21592697-21592716 MS.gene011362:intron 30.0%
! TAGCTGCTATTTAACAACAT+TGG - chr8.4:21592172-21592191 None:intergenic 30.0%
! TCATCTTTTCTAACATCAAG+CGG - chr8.4:21592594-21592613 None:intergenic 30.0%
! TGCATTTTCTTTCTCTTGAA+GGG - chr8.4:21592086-21592105 None:intergenic 30.0%
! TTGCATTTTCTTTCTCTTGA+AGG - chr8.4:21592087-21592106 None:intergenic 30.0%
!! ATTTTCCTTGTGAATGGATT+TGG + chr8.4:21592703-21592722 MS.gene011362:intron 30.0%
!!! ACTTTTTTATCAGCACTTGA+AGG + chr8.4:21592780-21592799 MS.gene011362:intron 30.0%
!!! TGTACCTCAATTTTTTCAGA+AGG + chr8.4:21592883-21592902 MS.gene011362:intron 30.0%
AAACTAAGCTTCAAGCTTGT+AGG - chr8.4:21593007-21593026 None:intergenic 35.0%
AAAGTGTGGGATACAAATAC+AGG + chr8.4:21593713-21593732 MS.gene011362:CDS 35.0%
AAATGCGCTATATGAAGACA+TGG + chr8.4:21593475-21593494 MS.gene011362:CDS 35.0%
ACGACCGTACTATAAAAGTT+TGG + chr8.4:21593954-21593973 MS.gene011362:CDS 35.0%
ACTTCTGATTGAATGTGTGT+TGG + chr8.4:21591651-21591670 MS.gene011362:intron 35.0%
ATCGACATGTCCAGAAAATA+TGG - chr8.4:21594062-21594081 None:intergenic 35.0%
CGACCGTACTATAAAAGTTT+GGG + chr8.4:21593955-21593974 MS.gene011362:CDS 35.0%
CGTTATACCATAGCAGAATT+TGG + chr8.4:21592198-21592217 MS.gene011362:intron 35.0%
CTATTCTCGATTGACAACAT+TGG + chr8.4:21592342-21592361 MS.gene011362:intron 35.0%
CTCAAACAACTTGTATGTGT+GGG + chr8.4:21593826-21593845 MS.gene011362:CDS 35.0%
GGAATGGAATTGTTTCAATC+TGG + chr8.4:21594467-21594486 MS.gene011362:CDS 35.0%
TATATGAAGACATGGTTCAG+CGG + chr8.4:21593483-21593502 MS.gene011362:CDS 35.0%
TCAATATCCCTTTCTCGAAA+TGG + chr8.4:21594157-21594176 MS.gene011362:CDS 35.0%
TCCTTAAGTTCAAGTCTTAC+TGG + chr8.4:21593737-21593756 MS.gene011362:CDS 35.0%
TCGAGAATAGCAGAAAATAG+TGG - chr8.4:21592332-21592351 None:intergenic 35.0%
TCTAGTAGATTGATTACTGG+GGG + chr8.4:21593674-21593693 MS.gene011362:CDS 35.0%
TGCAATTTCTGATATGCAGA+AGG + chr8.4:21592102-21592121 MS.gene011362:CDS 35.0%
TTAACAGAGTGGCTTCTAAT+TGG + chr8.4:21594269-21594288 MS.gene011362:CDS 35.0%
TTCAGAATGTTGCTCGATAT+TGG + chr8.4:21591842-21591861 MS.gene011362:intron 35.0%
! AAAGTTTGGGACTTGATGAA+AGG + chr8.4:21593968-21593987 MS.gene011362:CDS 35.0%
! ATATTTTCTGGACATGTCGA+TGG + chr8.4:21594061-21594080 MS.gene011362:CDS 35.0%
! TCAATCGCAACTTGTTCTTT+CGG - chr8.4:21591342-21591361 None:intergenic 35.0%
! TTCAGAACTAAAAGCACAAC+TGG + chr8.4:21592513-21592532 MS.gene011362:CDS 35.0%
!!! AGCTTTTGAAACTCTAGACT+CGG - chr8.4:21592062-21592081 None:intergenic 35.0%
ACACACGGATAAAGTTTGTG+CGG + chr8.4:21593886-21593905 MS.gene011362:CDS 40.0%
AGAAAGGGATATTGACGTGA+CGG - chr8.4:21594152-21594171 None:intergenic 40.0%
AGAAGGAGTTGACAGAATTG+AGG + chr8.4:21592119-21592138 MS.gene011362:CDS 40.0%
AGCCTGTATTTGCAATTGGA+AGG - chr8.4:21593608-21593627 None:intergenic 40.0%
AGGATCGTTCAGTTAAAGTG+TGG + chr8.4:21593699-21593718 MS.gene011362:CDS 40.0%
ATTGTTCGTCGAAGTGAAGA+AGG + chr8.4:21593554-21593573 MS.gene011362:CDS 40.0%
CAAAGCCAAATCCATTCACA+AGG - chr8.4:21592711-21592730 None:intergenic 40.0%
CGATGGTAATCTTCGGTTAT+GGG + chr8.4:21594078-21594097 MS.gene011362:CDS 40.0%
GAAAGAGAAAGCAGAGAACT+TGG + chr8.4:21591970-21591989 MS.gene011362:CDS 40.0%
GACATGTCGATGGTAATCTT+CGG + chr8.4:21594071-21594090 MS.gene011362:CDS 40.0%
GCCAGTAAGACTTGAACTTA+AGG - chr8.4:21593741-21593760 None:intergenic 40.0%
GCCTGTATTTGCAATTGGAA+GGG - chr8.4:21593607-21593626 None:intergenic 40.0%
GCTATAGCACTATTGTGTAG+CGG + chr8.4:21592294-21592313 MS.gene011362:intron 40.0%
GCTCAAACAACTTGTATGTG+TGG + chr8.4:21593825-21593844 MS.gene011362:CDS 40.0%
GGATCGTTCAGTTAAAGTGT+GGG + chr8.4:21593700-21593719 MS.gene011362:CDS 40.0%
GGTTTGTCCAAATTCTGCTA+TGG - chr8.4:21592208-21592227 None:intergenic 40.0%
GTTATTAACTCTTGTAGCGC+CGG + chr8.4:21591627-21591646 MS.gene011362:intron 40.0%
TACAGGCTAAATGCACATGA+AGG + chr8.4:21593620-21593639 MS.gene011362:CDS 40.0%
TACTTCCACAACAAGTTGCT+CGG - chr8.4:21592038-21592057 None:intergenic 40.0%
TCCCTTCCAATTGCAAATAC+AGG + chr8.4:21593603-21593622 MS.gene011362:CDS 40.0%
TCGATGGTAATCTTCGGTTA+TGG + chr8.4:21594077-21594096 MS.gene011362:CDS 40.0%
TTCTTACGGACCCACAATAA+AGG - chr8.4:21591560-21591579 None:intergenic 40.0%
TTGGTTGCTTGGATAGTTGA+TGG + chr8.4:21591872-21591891 MS.gene011362:intron 40.0%
TTTATCCGTGTGTCCAGTAA+GGG - chr8.4:21593879-21593898 None:intergenic 40.0%
TTTGTTTGATGTGCGATCTC+TGG + chr8.4:21594219-21594238 MS.gene011362:CDS 40.0%
! ACACATTCAATCAGAAGTGC+CGG - chr8.4:21591649-21591668 None:intergenic 40.0%
! ATATTGCATTGACCAGTGGA+AGG + chr8.4:21594182-21594201 MS.gene011362:CDS 40.0%
! CTCGTCCAGTATACAACTTT+TGG + chr8.4:21592657-21592676 MS.gene011362:intron 40.0%
! CTGATGGATCTGTGCATATA+TGG + chr8.4:21594341-21594360 MS.gene011362:CDS 40.0%
! CTTGTGAATGGATTTGGCTT+TGG + chr8.4:21592709-21592728 MS.gene011362:intron 40.0%
! GATGGACAGACCATATTTTC+TGG + chr8.4:21594049-21594068 MS.gene011362:CDS 40.0%
! GGAAATATTGCATTGACCAG+TGG + chr8.4:21594178-21594197 MS.gene011362:CDS 40.0%
! TATTGCATTGACCAGTGGAA+GGG + chr8.4:21594183-21594202 MS.gene011362:CDS 40.0%
! TGGTCTAGAACAACTTGCAA+GGG + chr8.4:21593517-21593536 MS.gene011362:CDS 40.0%
!! GCTCGATATTGGTGTTAAGT+TGG + chr8.4:21591853-21591872 MS.gene011362:intron 40.0%
!! GTTCACATCATAGTCTTGTG+AGG - chr8.4:21593369-21593388 None:intergenic 40.0%
!! TTGGTGTTAAGTTGGTTGCT+TGG + chr8.4:21591861-21591880 MS.gene011362:intron 40.0%
!!! TGTTGTTGAAGGGTTTTGAG+TGG + chr8.4:21591948-21591967 MS.gene011362:intron 40.0%
AAACGCCATCTTCTCGAAGA+AGG + chr8.4:21591393-21591412 MS.gene011362:CDS 45.0%
ACAACAATCATGTTGCCGCT+GGG + chr8.4:21594314-21594333 MS.gene011362:CDS 45.0%
ACAACTTGCAAGGGAACAAG+TGG + chr8.4:21593526-21593545 MS.gene011362:CDS 45.0%
AGAAGCCACTCTGTTAACTG+TGG - chr8.4:21594266-21594285 None:intergenic 45.0%
AGCGCATTTGCCTAAAGAGA+TGG - chr8.4:21593464-21593483 None:intergenic 45.0%
AGGCTAAATGCACATGAAGG+AGG + chr8.4:21593623-21593642 MS.gene011362:CDS 45.0%
AGGTAAATGACACTTGCCAG+TGG - chr8.4:21593035-21593054 None:intergenic 45.0%
ATTCCTGTCAGCTGAAGCTA+GGG - chr8.4:21594452-21594471 None:intergenic 45.0%
CTTCTGTTCTCTGTTGTAGC+TGG + chr8.4:21594410-21594429 MS.gene011362:CDS 45.0%
CTTTATCCGTGTGTCCAGTA+AGG - chr8.4:21593880-21593899 None:intergenic 45.0%
GAAAGCAGAGAACTTGGAAG+TGG + chr8.4:21591976-21591995 MS.gene011362:CDS 45.0%
GCGCATTTGCCTAAAGAGAT+GGG - chr8.4:21593463-21593482 None:intergenic 45.0%
GGCAAGTTGTGCAATCTTGA+CGG + chr8.4:21592730-21592749 MS.gene011362:intron 45.0%
GTACGTACCTGAGAAACGAT+GGG - chr8.4:21591453-21591472 None:intergenic 45.0%
GTGCAAATTATCCCTTCCAC+TGG - chr8.4:21594197-21594216 None:intergenic 45.0%
GTTCACAACACAGTCTCGTT+AGG - chr8.4:21593225-21593244 None:intergenic 45.0%
TAGATTGATTACTGGGGGAC+AGG + chr8.4:21593679-21593698 MS.gene011362:CDS 45.0%
TATGGGACATTCAGAGTGGA+AGG + chr8.4:21594095-21594114 MS.gene011362:CDS 45.0%
TATGTGTGGGATCTTAGCTC+AGG + chr8.4:21593839-21593858 MS.gene011362:CDS 45.0%
TCATACCCTTACTGGACACA+CGG + chr8.4:21593871-21593890 MS.gene011362:CDS 45.0%
TGAGATCCAATACTGAGCCA+AGG - chr8.4:21593766-21593785 None:intergenic 45.0%
TGTTGTAGCTGGAGTGGAAT+GGG + chr8.4:21594421-21594440 MS.gene011362:CDS 45.0%
TTCTAGACCCATCGTTTCTC+AGG + chr8.4:21591443-21591462 MS.gene011362:CDS 45.0%
TTGGTGTGAAATTGCTGAGC+AGG - chr8.4:21593055-21593074 None:intergenic 45.0%
TTGTCCAATAGCGGCTATAG+TGG + chr8.4:21592264-21592283 MS.gene011362:intron 45.0%
! CTAGAAAGTGCGAGAAAAGC+AGG - chr8.4:21591429-21591448 None:intergenic 45.0%
! GTGGTCTAGAACAACTTGCA+AGG + chr8.4:21593516-21593535 MS.gene011362:CDS 45.0%
! TGTGTCCAGTAAGGGTATGA+CGG - chr8.4:21593871-21593890 None:intergenic 45.0%
! TGTTGGTGTCTGACATGTGT+CGG + chr8.4:21591668-21591687 MS.gene011362:intron 45.0%
! TTGTGCGGTTGATGTTAGCA+AGG + chr8.4:21593901-21593920 MS.gene011362:CDS 45.0%
!! CAAAGAGCATTGCAGTTGCT+GGG - chr8.4:21594019-21594038 None:intergenic 45.0%
!! GTTATCCGAGCAACTTGTTG+TGG + chr8.4:21592030-21592049 MS.gene011362:CDS 45.0%
AAATGACACTTGCCAGTGGC+AGG - chr8.4:21593031-21593050 None:intergenic 50.0%
AAGACATGGTTCAGCGGCTA+AGG + chr8.4:21593489-21593508 MS.gene011362:CDS 50.0%
ACGAGCCACAGTTAACAGAG+TGG + chr8.4:21594258-21594277 MS.gene011362:CDS 50.0%
AGACATGGTTCAGCGGCTAA+GGG + chr8.4:21593490-21593509 MS.gene011362:CDS 50.0%
AGATCCATCAGTAGACCCAG+CGG - chr8.4:21594332-21594351 None:intergenic 50.0%
AGCACCACTATAGCCGCTAT+TGG - chr8.4:21592271-21592290 None:intergenic 50.0%
CATTCCTGTCAGCTGAAGCT+AGG - chr8.4:21594453-21594472 None:intergenic 50.0%
CGGTTATGGGACATTCAGAG+TGG + chr8.4:21594091-21594110 MS.gene011362:CDS 50.0%
CTAGCTTCAGCTGACAGGAA+TGG + chr8.4:21594451-21594470 MS.gene011362:CDS 50.0%
CTGTTGTAGCTGGAGTGGAA+TGG + chr8.4:21594420-21594439 MS.gene011362:CDS 50.0%
CTTGCAAGGGAACAAGTGGA+TGG + chr8.4:21593530-21593549 MS.gene011362:CDS 50.0%
GACAACAATCATGTTGCCGC+TGG + chr8.4:21594313-21594332 MS.gene011362:CDS 50.0%
GCGGCAACATGATTGTTGTC+TGG - chr8.4:21594313-21594332 None:intergenic 50.0%
GCTCTTTGCTTCAGCACAGA+TGG + chr8.4:21594031-21594050 MS.gene011362:CDS 50.0%
GGATGCTGAACGCCTAAATG+AGG + chr8.4:21592615-21592634 MS.gene011362:CDS 50.0%
GGTACGTACCTGAGAAACGA+TGG - chr8.4:21591454-21591473 None:intergenic 50.0%
GTTCTCTGTTGTAGCTGGAG+TGG + chr8.4:21594415-21594434 MS.gene011362:CDS 50.0%
TTCCTGTCAGCTGAAGCTAG+GGG - chr8.4:21594451-21594470 None:intergenic 50.0%
! GTGCGATCTCTGGAAGTTTG+TGG + chr8.4:21594229-21594248 MS.gene011362:CDS 50.0%
! TCAAGTCTTACTGGCTGCCT+TGG + chr8.4:21593746-21593765 MS.gene011362:CDS 50.0%
!! AAGCTTAGTTTGCCTGCCAC+TGG + chr8.4:21593016-21593035 MS.gene011362:intron 50.0%
!! GCAAAGAGCATTGCAGTTGC+TGG - chr8.4:21594020-21594039 None:intergenic 50.0%
AACCCCTAGCTTCAGCTGAC+AGG + chr8.4:21594446-21594465 MS.gene011362:CDS 55.0%
CGAGTCCGTCATACCCTTAC+TGG + chr8.4:21593863-21593882 MS.gene011362:CDS 55.0%
GAGCGCCTTCTTCGAGAAGA+TGG - chr8.4:21591401-21591420 None:intergenic 55.0%
GAGTGGAAGGCTGCTAAGTG+AGG + chr8.4:21594108-21594127 MS.gene011362:CDS 55.0%
TGGCTGCCTTGGCTCAGTAT+TGG + chr8.4:21593757-21593776 MS.gene011362:CDS 55.0%
GTTCAGCGGCTAAGGGCTAG+TGG + chr8.4:21593497-21593516 MS.gene011362:CDS 60.0%
GTTGCCGCTGGGTCTACTGA+TGG + chr8.4:21594325-21594344 MS.gene011362:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 21591321 21594495 21591321 ID=MS.gene011362
chr8.4 mRNA 21591321 21594495 21591321 ID=MS.gene011362.t1;Parent=MS.gene011362
chr8.4 exon 21591321 21591464 21591321 ID=MS.gene011362.t1.exon1;Parent=MS.gene011362.t1
chr8.4 CDS 21591321 21591464 21591321 ID=cds.MS.gene011362.t1;Parent=MS.gene011362.t1
chr8.4 exon 21591959 21592140 21591959 ID=MS.gene011362.t1.exon2;Parent=MS.gene011362.t1
chr8.4 CDS 21591959 21592140 21591959 ID=cds.MS.gene011362.t1;Parent=MS.gene011362.t1
chr8.4 exon 21592441 21592636 21592441 ID=MS.gene011362.t1.exon3;Parent=MS.gene011362.t1
chr8.4 CDS 21592441 21592636 21592441 ID=cds.MS.gene011362.t1;Parent=MS.gene011362.t1
chr8.4 exon 21593473 21594495 21593473 ID=MS.gene011362.t1.exon4;Parent=MS.gene011362.t1
chr8.4 CDS 21593473 21594495 21593473 ID=cds.MS.gene011362.t1;Parent=MS.gene011362.t1
Gene Sequence

>MS.gene011362

ATGGTGAAAACTGGAAGACCGAAAGAACAAGTTGCGATTGATGCAATCAATTATGCTATGAAAGCTCTCAAGAAACGCCATCTTCTCGAAGAAGGCGCTCATGCTCCTGCTTTTCTCGCACTTTCTAGACCCATCGTTTCTCAGGTACGTACCCTTTCATTAACAATTTTCATTTTTTTTAGGGATTTTTATCTTATTTATTTATCACACATGAATATCCATTTTTTTTATTGTTTCCTTTATTGTGGGTCCGTAAGAAATTAGTTCAATCATGAGTTCCAAAAGAATAGTGCTTAGTAGTTAATAGTTATTAACTCTTGTAGCGCCGGCACTTCTGATTGAATGTGTGTTGGTGTCTGACATGTGTCGGTGTCGAACACTCCATATGTGATCACATTGAATTATGTCATTTTTTCAAATTATTATCAGTGTCAACTCGCGAGTGTCTGTGTTTCATAAGTTGTGTACAAATTTACCTATTTTTAACTGTGGAAAGTTATTGGCTTTTGATGATGTCATATTTCAGAATGTTGCTCGATATTGGTGTTAAGTTGGTTGCTTGGATAGTTGATGGTTGTTTATGTGTTACAATTGTATTTTTGTTTTGTTGATTAAGTGATGTATTTATGTTGTTGAAGGGTTTTGAGTGGAAAGAGAAAGCAGAGAACTTGGAAGTGGAACTTCAGCAATGCTATAAAGCTCAATCTCGGTTATCCGAGCAACTTGTTGTGGAAGTAGCCGAGTCTAGAGTTTCAAAAGCTTCCCTTCAAGAGAAAGAAAATGCAATTTCTGATATGCAGAAGGAGTTGACAGAATTGAGGTTGCTTGATGTTTCTTATATGTTGTAACCAATGTTGTTAAATAGCAGCTATAGCACCGTTATACCATAGCAGAATTTGGACAAACCGCTATAGTTTCAAGATACACTATTTAGTACAAAATGTTGTCCAATAGCGGCTATAGTGGTGCTATTGCTATAGCACTATTGTGTAGCGGAATTTTAGAAATCCACTATTTTCTGCTATTCTCGATTGACAACATTGGTTGTAACCGCGAACAACATTTTTTTTAGTCTGCGATAAATGAGTTTTTTTACTTAAATTAATGTTGCAAAATGCAGGGATGAGTGCTCTCAATTAAAGACAGATTTGGAGCAAAAGACTAAAGAAGTAGAATTAGTTCTGAGCGAGAATTCAGAACTAAAAGCACAACTGGAGCAGATGACTACTAAAGCTAATGAAGCTGAAGCTGAAAATAAGATGTTGATTGACCGCTTGATGTTAGAAAAGATGAAGGATGCTGAACGCCTAAATGAGGTATAATACAATCTCATATTCTCGTCCAGTATACAACTTTTGGAACTAGATATTAGTTTTCTATACATTTTCCTTGTGAATGGATTTGGCTTTGGCAAGTTGTGCAATCTTGACGGAAATACTCTTAGAGCCCGTTTAATCGAACTTTTTTATCAGCACTTGAAGGAGAAGAAAGTTGAAATGAATTTGCAATGGTTATTTGCCTTGTCAAAGATAGTTGTTTGACTGTAGGTAGAAATTAGTTTCTGTACCTCAATTTTTTCAGAAGGTTCTGTATTTAAAATTATGCATCAACACCTATAAACTTATAAGAGTTTTTTCATAAGTTAGTAATTAAATGTTGAATATTTATCTTATTTGAGAAGCTCCTACAAGCTTGAAGCTTAGTTTGCCTGCCACTGGCAAGTGTCATTTACCTGCTCAGCAATTTCACACCAACATTTGATTTGATGTACTTCTAATTTTTTTATGTTTTGAAATTTGAATCTTACTAGTTAGTAGTTTTTCTATTCAAAACTCATAGTGCATAATATTTGGATCACTGTAATAGTTACATTAGTAGTTTATATTTCTTACATGTAGAGTCCTAACGAGACTGTGTTGTGAACAAATTCTAGTTTCATTTAATATTACCAGTTAGTACTTTTTCTTTTCAAAACTTGTAGTGCATAATATATTTGAATCACTGTGATAGTTACATTAGTAGATTATATTTCTTACATGTAGATTCCTCACAAGACTATGATGTGAACAAGTTCTAGTTGCATATAATTGTAATTTGTAAATAACAACTTATGTTGCCGATTCAACTAATAATACATTTCCCATCTCTTTAGGCAAATGCGCTATATGAAGACATGGTTCAGCGGCTAAGGGCTAGTGGTCTAGAACAACTTGCAAGGGAACAAGTGGATGGAATTGTTCGTCGAAGTGAAGAAGGTGCTGAATTCTTTTCAGAGTCAAACATCCCTTCCAATTGCAAATACAGGCTAAATGCACATGAAGGAGGTTGTGCTTCCTTATTGTTCGAGTACAATTCTAGTAGATTGATTACTGGGGGACAGGATCGTTCAGTTAAAGTGTGGGATACAAATACAGGATCCTTAAGTTCAAGTCTTACTGGCTGCCTTGGCTCAGTATTGGATCTCACTATCACCAATGATAATCGATCTGTTATTGCTGCGAGCAGCTCAAACAACTTGTATGTGTGGGATCTTAGCTCAGGTCGAGTCCGTCATACCCTTACTGGACACACGGATAAAGTTTGTGCGGTTGATGTTAGCAAGGTTTCGAGTCGTCTTGTTGTCAGTGCAGCTTACGACCGTACTATAAAAGTTTGGGACTTGATGAAAGGTTACTGCACAAACACAATCATGTTTCCCAGCAACTGCAATGCTCTTTGCTTCAGCACAGATGGACAGACCATATTTTCTGGACATGTCGATGGTAATCTTCGGTTATGGGACATTCAGAGTGGAAGGCTGCTAAGTGAGGTTGCTGCACATTCACACGCCGTCACGTCAATATCCCTTTCTCGAAATGGAAATATTGCATTGACCAGTGGAAGGGATAATTTGCACAATTTGTTTGATGTGCGATCTCTGGAAGTTTGTGGTACATTACGAGCCACAGTTAACAGAGTGGCTTCTAATTGGAGTCGCTCATGTATCAGTCCAGACAACAATCATGTTGCCGCTGGGTCTACTGATGGATCTGTGCATATATGGTCAATATCTAAAGGTGAAATAATCAGTACTCTGAAGGAGCATACTTCTTCTGTTCTCTGTTGTAGCTGGAGTGGAATGGGAAAACCCCTAGCTTCAGCTGACAGGAATGGAATTGTTTCAATCTGGTCATAA
Protein sequence

>MS.gene011362.t1

MVKTGRPKEQVAIDAINYAMKALKKRHLLEEGAHAPAFLALSRPIVSQGFEWKEKAENLEVELQQCYKAQSRLSEQLVVEVAESRVSKASLQEKENAISDMQKELTELRDECSQLKTDLEQKTKEVELVLSENSELKAQLEQMTTKANEAEAENKMLIDRLMLEKMKDAERLNEANALYEDMVQRLRASGLEQLAREQVDGIVRRSEEGAEFFSESNIPSNCKYRLNAHEGGCASLLFEYNSSRLITGGQDRSVKVWDTNTGSLSSSLTGCLGSVLDLTITNDNRSVIAASSSNNLYVWDLSSGRVRHTLTGHTDKVCAVDVSKVSSRLVVSAAYDRTIKVWDLMKGYCTNTIMFPSNCNALCFSTDGQTIFSGHVDGNLRLWDIQSGRLLSEVAAHSHAVTSISLSRNGNIALTSGRDNLHNLFDVRSLEVCGTLRATVNRVASNWSRSCISPDNNHVAAGSTDGSVHIWSISKGEIISTLKEHTSSVLCCSWSGMGKPLASADRNGIVSIWS