AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065050


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065050.t1 MTR_1g112200 97.619 210 5 0 1 210 1 210 8.48e-150 414
MS.gene065050.t1 MTR_1g060120 55.882 204 79 1 17 209 20 223 1.09e-80 240
MS.gene065050.t1 MTR_1g085500 42.857 210 113 3 7 209 7 216 1.35e-53 171
MS.gene065050.t1 MTR_7g111240 45.596 193 99 4 22 209 19 210 5.73e-53 169
MS.gene065050.t1 MTR_7g111240 44.898 196 99 4 22 209 19 213 2.58e-52 167
MS.gene065050.t1 MTR_1g085140 43.719 199 103 4 19 209 20 217 4.00e-51 165
MS.gene065050.t1 MTR_1g079490 44.554 202 97 6 19 207 18 217 6.39e-48 157
MS.gene065050.t1 MTR_6g005340 45.161 217 102 8 5 207 2 215 8.91e-48 156
MS.gene065050.t1 MTR_6g005330 44.907 216 101 8 7 207 3 215 9.30e-48 156
MS.gene065050.t1 MTR_6g005310 45.161 217 102 8 5 207 2 215 2.05e-47 155
MS.gene065050.t1 MTR_6g005360 44.907 216 104 7 5 207 2 215 3.80e-47 154
MS.gene065050.t1 MTR_4g017050 42.786 201 99 7 19 206 18 215 4.54e-44 147
MS.gene065050.t1 MTR_5g046410 42.786 201 99 7 19 206 18 215 5.29e-44 147
MS.gene065050.t1 MTR_5g046430 42.786 201 99 7 19 206 18 215 5.29e-44 147
MS.gene065050.t1 MTR_4g017030 42.786 201 99 7 19 206 18 215 5.29e-44 147
MS.gene065050.t1 MTR_4g017040 41.204 216 106 8 9 206 3 215 1.54e-43 145
MS.gene065050.t1 MTR_2g019250 42.786 201 99 7 19 206 18 215 2.71e-43 145
MS.gene065050.t1 MTR_1g023770 39.604 202 115 4 11 207 10 209 3.14e-43 144
MS.gene065050.t1 MTR_2g031270 42.021 188 101 5 27 207 27 213 5.07e-43 144
MS.gene065050.t1 MTR_2g030855 41.026 195 107 5 20 207 19 212 5.93e-43 144
MS.gene065050.t1 MTR_5g036570 40.722 194 107 4 22 207 26 219 5.32e-41 139
MS.gene065050.t1 MTR_2g041570 37.255 204 119 3 9 205 3 204 1.45e-38 132
MS.gene065050.t1 MTR_2g041580 37.438 203 118 3 9 204 3 203 1.49e-38 132
MS.gene065050.t1 MTR_2g030895 43.023 172 92 4 42 207 7 178 2.78e-38 130
MS.gene065050.t1 MTR_2g041550 37.313 201 121 2 9 204 3 203 2.93e-38 131
MS.gene065050.t1 MTR_6g005350 40.196 204 100 6 19 207 18 214 4.44e-38 131
MS.gene065050.t1 MTR_2g030865 42.442 172 93 4 42 207 7 178 5.79e-37 127
MS.gene065050.t1 MTR_8g020590 36.538 208 114 5 10 205 1 202 1.48e-36 127
MS.gene065050.t1 MTR_3g019710 34.783 207 124 5 8 207 3 205 2.72e-35 124
MS.gene065050.t1 MTR_8g020630 35.922 206 123 6 8 207 3 205 3.84e-35 123
MS.gene065050.t1 MTR_8g020610 33.010 206 129 4 8 207 1 203 1.77e-34 122
MS.gene065050.t1 MTR_2g086640 34.762 210 111 5 12 208 8 204 1.83e-34 122
MS.gene065050.t1 MTR_2g036120 32.843 204 132 2 8 207 3 205 2.89e-34 121
MS.gene065050.t1 MTR_2g045100 37.079 178 105 4 33 207 32 205 1.03e-33 120
MS.gene065050.t1 MTR_8g020650 37.968 187 107 6 27 207 22 205 1.94e-33 119
MS.gene065050.t1 MTR_3g019680 35.266 207 119 5 8 207 6 204 4.50e-33 118
MS.gene065050.t1 MTR_6g005380 38.916 203 107 6 7 197 9 206 1.34e-32 117
MS.gene065050.t1 MTR_3g019650 35.749 207 118 6 8 207 6 204 2.70e-32 116
MS.gene065050.t1 MTR_8g020670 34.483 203 124 5 11 207 6 205 3.36e-32 116
MS.gene065050.t1 MTR_4g010340 33.673 196 121 4 16 207 11 201 4.09e-32 115
MS.gene065050.t1 MTR_2g044040 32.683 205 131 4 8 207 3 205 1.20e-31 114
MS.gene065050.t1 MTR_4g052770 33.491 212 121 5 5 208 7 206 3.00e-31 113
MS.gene065050.t1 MTR_8g020763 34.706 170 107 3 40 207 19 186 3.96e-31 112
MS.gene065050.t1 MTR_2g086620 32.212 208 119 5 12 208 9 205 8.53e-31 112
MS.gene065050.t1 MTR_2g086630 32.212 208 119 5 12 208 9 205 9.19e-31 112
MS.gene065050.t1 MTR_8g020750 33.010 206 131 3 8 207 4 208 9.30e-31 112
MS.gene065050.t1 MTR_8g007300 34.759 187 114 2 22 200 25 211 1.60e-30 112
MS.gene065050.t1 MTR_3g096330 41.176 170 88 2 15 172 563 732 1.62e-30 118
MS.gene065050.t1 MTR_0284s0010 31.220 205 134 4 8 207 3 205 2.07e-30 111
MS.gene065050.t1 MTR_4g052780 38.125 160 95 2 53 208 47 206 2.87e-30 111
MS.gene065050.t1 MTR_8g020760 33.810 210 123 8 8 207 4 207 4.48e-29 108
MS.gene065050.t1 MTR_2g031300 41.875 160 68 4 42 196 7 146 8.63e-29 105
MS.gene065050.t1 MTR_7g011340 31.892 185 121 2 27 207 22 205 1.27e-28 107
MS.gene065050.t1 MTR_3g073170 35.263 190 102 6 29 207 27 206 1.64e-28 106
MS.gene065050.t1 MTR_5g031460 29.412 204 139 2 8 207 3 205 5.35e-28 105
MS.gene065050.t1 MTR_8g020770 30.583 206 136 3 8 207 4 208 1.25e-27 104
MS.gene065050.t1 MTR_2g019780 32.086 187 112 7 29 207 29 208 4.23e-25 97.8
MS.gene065050.t1 MTR_3g019810 28.846 208 133 5 3 204 104 302 1.04e-20 88.2
MS.gene065050.t1 MTR_4g027415 33.333 123 75 4 27 144 22 142 3.11e-14 67.4
MS.gene065050.t1 MTR_2g072560 31.579 114 72 3 8 116 3 115 6.59e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065050.t1 AT3G10080 59.709 206 71 3 13 209 19 221 2.34e-85 252
MS.gene065050.t1 AT3G10080 59.709 206 71 3 13 209 90 292 1.08e-84 253
MS.gene065050.t1 AT1G02335 42.791 215 115 4 1 207 1 215 3.12e-56 178
MS.gene065050.t1 AT3G04200 45.540 213 103 5 8 209 14 224 3.17e-54 173
MS.gene065050.t1 AT1G18980 45.192 208 105 4 7 207 13 218 5.56e-53 169
MS.gene065050.t1 AT1G18970 42.788 208 110 4 7 207 13 218 1.41e-52 169
MS.gene065050.t1 AT5G38960 44.878 205 93 6 19 207 22 222 2.54e-51 165
MS.gene065050.t1 AT5G39160 41.827 208 111 5 7 207 11 215 3.10e-51 165
MS.gene065050.t1 AT5G39160 41.315 213 109 6 7 207 11 219 3.49e-51 165
MS.gene065050.t1 AT5G39130 40.845 213 110 6 7 207 11 219 5.22e-51 164
MS.gene065050.t1 AT3G62020 43.204 206 109 4 12 209 12 217 5.37e-51 164
MS.gene065050.t1 AT5G39190 41.315 213 109 6 7 207 11 219 6.01e-51 164
MS.gene065050.t1 AT5G26700 40.758 211 112 5 7 209 6 211 1.07e-50 163
MS.gene065050.t1 AT3G05950 44.393 214 104 6 7 208 12 222 6.89e-50 162
MS.gene065050.t1 AT1G09560 43.367 196 102 4 22 209 23 217 7.20e-50 161
MS.gene065050.t1 AT5G39110 41.475 217 112 6 5 207 5 220 1.73e-49 160
MS.gene065050.t1 AT3G05930 38.710 217 125 3 1 209 1 217 6.80e-49 159
MS.gene065050.t1 AT5G39180 41.315 213 111 5 8 207 8 219 7.36e-49 159
MS.gene065050.t1 AT5G39150 41.315 213 111 5 8 207 8 219 2.22e-48 158
MS.gene065050.t1 AT5G39120 41.096 219 112 6 5 207 2 219 5.65e-48 157
MS.gene065050.t1 AT3G62020 45.699 186 95 4 28 209 5 188 2.21e-47 154
MS.gene065050.t1 AT5G38910 41.860 215 110 6 6 207 7 219 7.18e-47 154
MS.gene065050.t1 AT5G38940 41.919 198 102 5 22 207 25 221 1.11e-46 154
MS.gene065050.t1 AT3G04170 42.254 213 109 6 8 210 13 221 1.64e-46 153
MS.gene065050.t1 AT5G38940 41.919 198 102 5 22 207 49 245 1.67e-46 154
MS.gene065050.t1 AT5G39160 39.614 207 99 6 7 207 11 197 7.79e-46 150
MS.gene065050.t1 AT5G39190 39.614 207 99 6 7 207 11 197 1.18e-45 150
MS.gene065050.t1 AT4G14630 40.000 200 106 5 21 207 24 222 2.64e-45 150
MS.gene065050.t1 AT3G04180 39.500 200 110 4 20 208 23 222 2.15e-44 147
MS.gene065050.t1 AT3G04150 39.623 212 115 5 8 208 13 222 3.18e-44 147
MS.gene065050.t1 AT5G38930 40.704 199 105 5 21 207 24 221 7.19e-44 146
MS.gene065050.t1 AT3G04190 40.097 207 99 6 20 208 23 222 2.79e-43 145
MS.gene065050.t1 AT3G04150 38.222 225 113 6 8 208 13 235 1.53e-42 144
MS.gene065050.t1 AT5G20630 38.798 183 107 2 27 204 22 204 2.25e-40 137
MS.gene065050.t1 AT1G10460 35.678 199 114 5 22 207 18 215 2.16e-38 132
MS.gene065050.t1 AT5G61750 38.983 177 101 3 9 178 5 181 8.03e-35 123
MS.gene065050.t1 AT1G72610 37.037 189 102 3 27 204 19 201 4.16e-34 121
MS.gene065050.t1 AT1G74820 31.390 223 134 6 2 208 7 226 1.32e-33 120
MS.gene065050.t1 AT5G38910 35.211 213 90 6 6 207 7 182 3.47e-30 110
MS.gene065050.t1 AT5G38950 50.794 63 29 1 138 198 2 64 4.72e-13 63.2

Find 20 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGATTAAAGATGAGCTTT+TGG 0.232327 1.2:+83023204 MS.gene065050:CDS
GAATTTGTTATCTTCTGATT+TGG 0.267937 1.2:-83022756 None:intergenic
TTCTGATCCGGATCCTGTTA+TGG 0.323739 1.2:+83022721 MS.gene065050:CDS
CTAGTATCTTTGAAGTTTCC+TGG 0.326307 1.2:-83022839 None:intergenic
GTGAATTCAAATGTGTTTCC+AGG 0.358885 1.2:+83022878 MS.gene065050:CDS
TTTGGAGAAAGGTGAGGTTA+TGG 0.393881 1.2:+83023060 MS.gene065050:CDS
TGAAAATGCACAAGTCCTCT+TGG 0.414763 1.2:-83023088 None:intergenic
GCTATGCAGTAATCCATAAC+AGG 0.434646 1.2:-83022734 None:intergenic
ATAGCAACTTCTGTTGCTCT+TGG 0.444865 1.2:-83022974 None:intergenic
CAGTAATCCATAACAGGATC+CGG 0.469062 1.2:-83022728 None:intergenic
TTTCCAGGTTTAAACACTCT+TGG 0.469660 1.2:+83022893 MS.gene065050:CDS
CAGCAATGATGAAACACATA+AGG 0.504374 1.2:-83022691 None:intergenic
ACACATAAGGACTAAAAGAA+TGG 0.521199 1.2:-83022678 None:intergenic
AGAAGTTGCTATTGTGTTGG+AGG 0.523101 1.2:+83022985 MS.gene065050:CDS
AACAGAAGTTGCTATTGTGT+TGG 0.541553 1.2:+83022982 MS.gene065050:CDS
GAAGTTGCTATTGTGTTGGA+GGG 0.567803 1.2:+83022986 MS.gene065050:CDS
ACTCCAAGAGTGTTTAAACC+TGG 0.567991 1.2:-83022896 None:intergenic
GAAATCTTCAACTGTTGCTG+TGG 0.571728 1.2:-83022795 None:intergenic
AGCTTTCTAAGTTAAAGAAG+AGG 0.576087 1.2:+83023254 MS.gene065050:CDS
GTTGCTCTTGGATGAAAGTG+AGG 0.618955 1.2:-83022962 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTCCAAATGTTGTTTT+TGG + chr1.2:83023178-83023197 MS.gene065050:CDS 20.0%
!! AGATACTAGATTTTCTTCAA+TGG + chr1.2:83022853-83022872 MS.gene065050:CDS 25.0%
!! CAAGATTTTTGCTAAAGTTT+TGG + chr1.2:83023042-83023061 MS.gene065050:CDS 25.0%
!! GATACTAGATTTTCTTCAAT+GGG + chr1.2:83022854-83022873 MS.gene065050:CDS 25.0%
!!! GAATTTGTTATCTTCTGATT+TGG - chr1.2:83022759-83022778 None:intergenic 25.0%
AAACTACCAAAAATAGTAGC+AGG - chr1.2:83023133-83023152 None:intergenic 30.0%
ACACATAAGGACTAAAAGAA+TGG - chr1.2:83022681-83022700 None:intergenic 30.0%
AGCTTTCTAAGTTAAAGAAG+AGG + chr1.2:83023254-83023273 MS.gene065050:CDS 30.0%
TCAGATCCAAAAACAACATT+TGG - chr1.2:83023187-83023206 None:intergenic 30.0%
TTGGAGGGAAAAATTTATTC+AGG + chr1.2:83023001-83023020 MS.gene065050:CDS 30.0%
! TGAGATTAAAGATGAGCTTT+TGG + chr1.2:83023204-83023223 MS.gene065050:CDS 30.0%
!! AGTTTTGATAGTCAAAATCC+TGG + chr1.2:83023148-83023167 MS.gene065050:CDS 30.0%
!! TTTGGAATTTTCATCAAACC+AGG - chr1.2:83023169-83023188 None:intergenic 30.0%
!! TTTTCTGGTATAAAACTACC+AGG + chr1.2:83022821-83022840 MS.gene065050:CDS 30.0%
!!! GTTGAAGATTTCACTTTTTC+TGG + chr1.2:83022806-83022825 MS.gene065050:CDS 30.0%
!!! TTTGCTAAAGTTTTGGAGAA+AGG + chr1.2:83023049-83023068 MS.gene065050:CDS 30.0%
CAGCAATGATGAAACACATA+AGG - chr1.2:83022694-83022713 None:intergenic 35.0%
GTGAATTCAAATGTGTTTCC+AGG + chr1.2:83022878-83022897 MS.gene065050:CDS 35.0%
TTTCCAGGTTTAAACACTCT+TGG + chr1.2:83022893-83022912 MS.gene065050:CDS 35.0%
! AACAGAAGTTGCTATTGTGT+TGG + chr1.2:83022982-83023001 MS.gene065050:CDS 35.0%
! AGCTTTTGGACTTGATTCTA+AGG + chr1.2:83023231-83023250 MS.gene065050:CDS 35.0%
! GATGTTCCTGCTACTATTTT+TGG + chr1.2:83023124-83023143 MS.gene065050:CDS 35.0%
! GTGCATTTTCAGATGAATGT+TGG + chr1.2:83023100-83023119 MS.gene065050:CDS 35.0%
!! CTAGTATCTTTGAAGTTTCC+TGG - chr1.2:83022842-83022861 None:intergenic 35.0%
!! GAGCTTTTGGAAAAAGCTTT+TGG + chr1.2:83023217-83023236 MS.gene065050:CDS 35.0%
!! GTTAGAGCAGATTTTGATGT+TGG + chr1.2:83022926-83022945 MS.gene065050:CDS 35.0%
!!! TAAAGTTTTGGAGAAAGGTG+AGG + chr1.2:83023054-83023073 MS.gene065050:CDS 35.0%
ATAGCAACTTCTGTTGCTCT+TGG - chr1.2:83022977-83022996 None:intergenic 40.0%
CAGTAATCCATAACAGGATC+CGG - chr1.2:83022731-83022750 None:intergenic 40.0%
GAAATCTTCAACTGTTGCTG+TGG - chr1.2:83022798-83022817 None:intergenic 40.0%
GCTATGCAGTAATCCATAAC+AGG - chr1.2:83022737-83022756 None:intergenic 40.0%
TGAAAATGCACAAGTCCTCT+TGG - chr1.2:83023091-83023110 None:intergenic 40.0%
TTTGGAGAAAGGTGAGGTTA+TGG + chr1.2:83023060-83023079 MS.gene065050:CDS 40.0%
! AGAAGTTGCTATTGTGTTGG+AGG + chr1.2:83022985-83023004 MS.gene065050:CDS 40.0%
! GAAGTTGCTATTGTGTTGGA+GGG + chr1.2:83022986-83023005 MS.gene065050:CDS 40.0%
! GAGGTTATGGTTTTTCCAAG+AGG + chr1.2:83023073-83023092 MS.gene065050:CDS 40.0%
!! ACTCCAAGAGTGTTTAAACC+TGG - chr1.2:83022899-83022918 None:intergenic 40.0%
!! AGAGCAGATTTTGATGTTGG+TGG + chr1.2:83022929-83022948 MS.gene065050:CDS 40.0%
!!! TGCTGCTTTTTGTTCTGATC+CGG + chr1.2:83022709-83022728 MS.gene065050:CDS 40.0%
GTTGCTCTTGGATGAAAGTG+AGG - chr1.2:83022965-83022984 None:intergenic 45.0%
TTCTGATCCGGATCCTGTTA+TGG + chr1.2:83022721-83022740 MS.gene065050:CDS 45.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 83022659 83023291 83022659 ID=MS.gene065050
chr1.2 mRNA 83022659 83023291 83022659 ID=MS.gene065050.t1;Parent=MS.gene065050
chr1.2 exon 83022659 83023291 83022659 ID=MS.gene065050.t1.exon1;Parent=MS.gene065050.t1
chr1.2 CDS 83022659 83023291 83022659 ID=cds.MS.gene065050.t1;Parent=MS.gene065050.t1
Gene Sequence

>MS.gene065050

ATGATCAACAACAAAGTAACCATTCTTTTAGTCCTTATGTGTTTCATCATTGCTGCTTTTTGTTCTGATCCGGATCCTGTTATGGATTACTGCATAGCCAAATCAGAAGATAACAAATTCAGTTGCAAAAACTCATCCACAGCAACAGTTGAAGATTTCACTTTTTCTGGTATAAAACTACCAGGAAACTTCAAAGATACTAGATTTTCTTCAATGGGAGTGAATTCAAATGTGTTTCCAGGTTTAAACACTCTTGGAGTTTCATTTGTTAGAGCAGATTTTGATGTTGGTGGTGTTAATGTTCCTCACTTTCATCCAAGAGCAACAGAAGTTGCTATTGTGTTGGAGGGAAAAATTTATTCAGGTTTTGTTGATACTAAGAACAAGATTTTTGCTAAAGTTTTGGAGAAAGGTGAGGTTATGGTTTTTCCAAGAGGACTTGTGCATTTTCAGATGAATGTTGGTGATGTTCCTGCTACTATTTTTGGTAGTTTTGATAGTCAAAATCCTGGTTTGATGAAAATTCCAAATGTTGTTTTTGGATCTGAGATTAAAGATGAGCTTTTGGAAAAAGCTTTTGGACTTGATTCTAAGGAGCTTTCTAAGTTAAAGAAGAGGTTTACTCCTAGCTAA

Protein sequence

>MS.gene065050.t1

MINNKVTILLVLMCFIIAAFCSDPDPVMDYCIAKSEDNKFSCKNSSTATVEDFTFSGIKLPGNFKDTRFSSMGVNSNVFPGLNTLGVSFVRADFDVGGVNVPHFHPRATEVAIVLEGKIYSGFVDTKNKIFAKVLEKGEVMVFPRGLVHFQMNVGDVPATIFGSFDSQNPGLMKIPNVVFGSEIKDELLEKAFGLDSKELSKLKKRFTPS