AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065301

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065301.t1 MTR_7g029455 95.610 205 9 0 1 205 1 205 5.23e-147 409
MS.gene065301.t1 MTR_7g029455 92.891 211 9 1 1 205 1 211 2.19e-144 403
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065301.t1 AT3G12530 63.902 205 73 1 1 205 1 204 8.94e-94 275
MS.gene065301.t1 AT3G12530 63.902 205 73 1 1 205 1 204 8.94e-94 275
MS.gene065301.t1 AT3G12530 64.516 186 65 1 20 205 1 185 4.97e-85 252
MS.gene065301.t1 AT3G12530 65.986 147 50 0 1 147 1 147 4.83e-70 212

Find 53 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAAAGCTCTCCCAATAAA+TGG 0.205932 1.2:+46977938 None:intergenic
AATATTGTTCGCCCATTTAT+TGG 0.207398 1.2:-46977950 MS.gene065301:CDS
TCCGAAACTCTCTCTGGGTC+TGG 0.302087 1.2:+46977881 None:intergenic
ATAATTATGTATAACTTGTT+TGG 0.321106 1.2:-46977333 MS.gene065301:CDS
TGACAATTGCAGGGAGATTT+CGG 0.324639 1.2:-46980618 MS.gene065301:intron
CATCGTGACTAATCTCAAAA+TGG 0.330451 1.2:-46980746 MS.gene065301:CDS
TCTGGGTCTGGGTTCAAGTC+TGG 0.338635 1.2:+46977893 None:intergenic
GTAACAATCTGCGGAGTAAA+TGG 0.340928 1.2:+46980594 None:intergenic
CGTCTATCCGAAACTCTCTC+TGG 0.345540 1.2:+46977875 None:intergenic
GCAAATGCTCCGACATCTCT+TGG 0.362089 1.2:+46979816 None:intergenic
ATATTGTTCGCCCATTTATT+GGG 0.393276 1.2:-46977949 MS.gene065301:CDS
GAAATTTCTACATAATGAAA+TGG 0.395437 1.2:+46979794 None:intergenic
ATGAAAAGAGTGAAAGTTCA+TGG 0.404231 1.2:+46980902 None:intergenic
CTCCCATGTACTTCTCAAAT+AGG 0.416880 1.2:-46977241 None:intergenic
ACATACTCTTGGGGCATTGT+GGG 0.433438 1.2:+46977841 None:intergenic
GTATAACTTGTTTGGCAAGA+AGG 0.438248 1.2:-46977325 MS.gene065301:CDS
CCGAAACTCTCTCTGGGTCT+GGG 0.440746 1.2:+46977882 None:intergenic
TTGTTACTCAAGTACCGCTT+TGG 0.468205 1.2:-46980578 MS.gene065301:CDS
GAACTCACCATGTATGCATC+AGG 0.471100 1.2:+46979646 None:intergenic
CCCAGACCCAGAGAGAGTTT+CGG 0.471200 1.2:-46977882 MS.gene065301:CDS
TTTGGTTGGCAATTGCTCTC+AGG 0.473747 1.2:-46980560 MS.gene065301:CDS
TTGAGGACATCAGAGATGTA+AGG 0.475567 1.2:-46979529 MS.gene065301:CDS
CAACATTCCTGATGCATACA+TGG 0.483264 1.2:-46979653 MS.gene065301:intron
GCTCCATTGTCCCTCCTCAA+TGG 0.483624 1.2:-46980524 MS.gene065301:CDS
GTAAAGCTCTCCCAATAAAT+GGG 0.485912 1.2:+46977939 None:intergenic
TAAAGTTGAAACTGACTTGG+AGG 0.491249 1.2:-46979501 MS.gene065301:CDS
CAAATGCTCCGACATCTCTT+GGG 0.494044 1.2:+46979817 None:intergenic
AACATACTCTTGGGGCATTG+TGG 0.508642 1.2:+46977840 None:intergenic
TTTCAAAGAGAAGAGATGGC+TGG 0.523532 1.2:-46980934 None:intergenic
TTCAAAGAGAAGAGATGGCT+GGG 0.527242 1.2:-46980933 None:intergenic
TCATAAAGTTGAAACTGACT+TGG 0.529908 1.2:-46979504 MS.gene065301:CDS
GTCTATCCGAAACTCTCTCT+GGG 0.530720 1.2:+46977876 None:intergenic
CTACCTATTTGAGAAGTACA+TGG 0.534207 1.2:+46977238 None:intergenic
ACTGACTTGGAGGCATTCAA+TGG 0.547053 1.2:-46979491 MS.gene065301:CDS
TAAGTTGACACAAGTGTTGG+AGG 0.551466 1.2:-46979852 MS.gene065301:CDS
GGGGCATTGTGGGCAACTTG+TGG 0.560211 1.2:+46977851 None:intergenic
TACTCAAGTACCGCTTTGGT+TGG 0.563566 1.2:-46980574 MS.gene065301:CDS
TGGTCGTACAATAGCTGTGA+AGG 0.568663 1.2:-46979471 MS.gene065301:intron
GAAGGTGAGGTCTCTAATTG+AGG 0.573692 1.2:-46979546 MS.gene065301:intron
CATCCATTGAGGAGGGACAA+TGG 0.577418 1.2:+46980521 None:intergenic
AGATAAGTTGACACAAGTGT+TGG 0.580530 1.2:-46979855 MS.gene065301:CDS
GCGAAACTCCCAAGAGATGT+CGG 0.605151 1.2:-46979825 MS.gene065301:CDS
GCAATTGCTCTCAGGAAGAG+AGG 0.614416 1.2:-46980552 MS.gene065301:CDS
CCTCCTCAATGGATGACTGT+TGG 0.615572 1.2:-46980513 MS.gene065301:intron
CATCGCTGAAGACGAGATTG+TGG 0.631390 1.2:-46980770 MS.gene065301:CDS
ATCAAACAAACATACTCTTG+GGG 0.651202 1.2:+46977832 None:intergenic
TACCTATTTGAGAAGTACAT+GGG 0.653958 1.2:+46977239 None:intergenic
TTACCAACAGTCATCCATTG+AGG 0.655858 1.2:+46980510 None:intergenic
CAACAGTCATCCATTGAGGA+GGG 0.658288 1.2:+46980514 None:intergenic
CCAACAGTCATCCATTGAGG+AGG 0.658541 1.2:+46980513 None:intergenic
CTTACAGAGATGAGACAGAG+AGG 0.676515 1.2:+46980721 None:intergenic
GGTACTTGAGTAACAATCTG+CGG 0.687743 1.2:+46980585 None:intergenic
AGAGCAATTGCCAACCAAAG+CGG 0.692803 1.2:+46980564 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTAAAAATAAAAAATAGA+AGG + chr1.2:46978481-46978500 None:intergenic 10.0%
!!! ATATTGAATTTTTATATCTT+TGG + chr1.2:46980656-46980675 None:intergenic 10.0%
!!! GACTTTTTTTTTTATATATA+AGG - chr1.2:46978775-46978794 MS.gene065301:intron 10.0%
!!! TTGTATTTATTTTTTATTCA+AGG - chr1.2:46977868-46977887 MS.gene065301:CDS 10.0%
!! AAAGATAACATAAAAGTAAT+TGG + chr1.2:46977850-46977869 None:intergenic 15.0%
!! AAATAATGGTTTATACATAA+AGG + chr1.2:46978930-46978949 None:intergenic 15.0%
!! AACAAGAAATATAAAACAAA+TGG + chr1.2:46978122-46978141 None:intergenic 15.0%
!! ATTAAATATGAGAAAACAAA+AGG - chr1.2:46979217-46979236 MS.gene065301:intron 15.0%
!! TATATATATATTCTTCCAAA+TGG - chr1.2:46980756-46980775 MS.gene065301:CDS 15.0%
!!! ATAATTATGTATAACTTGTT+TGG - chr1.2:46980828-46980847 MS.gene065301:intron 15.0%
!!! ATATTTCTATTATAAAAGAG+AGG + chr1.2:46979598-46979617 None:intergenic 15.0%
!!! TATTTCTATTATAAAAGAGA+GGG + chr1.2:46979597-46979616 None:intergenic 15.0%
!!! TGTATTTTATTGAATGATAA+TGG - chr1.2:46979415-46979434 MS.gene065301:intron 15.0%
!!! TTAGTTGTTTTTTTTTAACA+AGG - chr1.2:46979824-46979843 MS.gene065301:CDS 15.0%
!!! TTGTATTTTAATTCTGATTT+GGG - chr1.2:46978056-46978075 MS.gene065301:intron 15.0%
!!! TTTTGTAATCAAATCAAATA+CGG + chr1.2:46978872-46978891 None:intergenic 15.0%
!! AATAACAACATAGTTGTATA+TGG + chr1.2:46977315-46977334 None:intergenic 20.0%
!! ACATAATGTTGTGAAAAATT+AGG - chr1.2:46977728-46977747 MS.gene065301:intron 20.0%
!! ATTATTTACACTTGAATAGA+AGG - chr1.2:46978943-46978962 MS.gene065301:intron 20.0%
!! CATAATGTTGTGAAAAATTA+GGG - chr1.2:46977729-46977748 MS.gene065301:intron 20.0%
!! GAAATTTCTACATAATGAAA+TGG + chr1.2:46978370-46978389 None:intergenic 20.0%
!! GATTAAAAATTTATCTGCAA+TGG - chr1.2:46980183-46980202 MS.gene065301:intron 20.0%
!! GGAATAGAACTTAAAAAATA+AGG + chr1.2:46980040-46980059 None:intergenic 20.0%
!! TTCTATTCAAGTGTAAATAA+TGG + chr1.2:46978944-46978963 None:intergenic 20.0%
!! TTTCTCATATTTAATTACAG+TGG + chr1.2:46979212-46979231 None:intergenic 20.0%
!! TTTGTAATCAAATCAAATAC+GGG + chr1.2:46978871-46978890 None:intergenic 20.0%
!!! ACATTTTACATCATAATTCT+TGG - chr1.2:46980151-46980170 MS.gene065301:intron 20.0%
!!! CAAACTAACAAATTTTTAAC+TGG + chr1.2:46978242-46978261 None:intergenic 20.0%
!!! CTTGTATTTTAATTCTGATT+TGG - chr1.2:46978055-46978074 MS.gene065301:intron 20.0%
!!! TTTTTTTAATGACAATTGCA+GGG - chr1.2:46977534-46977553 MS.gene065301:intron 20.0%
!!! TTTTTTTTAATGACAATTGC+AGG - chr1.2:46977533-46977552 MS.gene065301:intron 20.0%
! ATATATTCTTCCAAATGGAA+TGG - chr1.2:46980761-46980780 MS.gene065301:CDS 25.0%
! ATATCAAACAAACATACTCT+TGG + chr1.2:46980334-46980353 None:intergenic 25.0%
! ATATTGTTAGAAAGATGGTT+AGG + chr1.2:46979548-46979567 None:intergenic 25.0%
! ATTACTGCAATAAGCATAAT+TGG - chr1.2:46977926-46977945 MS.gene065301:CDS 25.0%
! ATTCAGAATCTAATCTATTC+TGG + chr1.2:46979307-46979326 None:intergenic 25.0%
! CCATATCTCTTTCATATATT+AGG - chr1.2:46979343-46979362 MS.gene065301:intron 25.0%
! CCTAATATATGAAAGAGATA+TGG + chr1.2:46979346-46979365 None:intergenic 25.0%
! GAACTTTCTTAAAAATGATC+TGG + chr1.2:46979190-46979209 None:intergenic 25.0%
! GAGAAATATTGTTAGAAAGA+TGG + chr1.2:46979553-46979572 None:intergenic 25.0%
! GTAATTGGAACAAAAATGTA+TGG + chr1.2:46977835-46977854 None:intergenic 25.0%
! TAGAAATATCCCTAAAATCA+AGG - chr1.2:46979609-46979628 MS.gene065301:intron 25.0%
! TATCAAACAAACATACTCTT+GGG + chr1.2:46980333-46980352 None:intergenic 25.0%
! TGAAAAATTAGGGATAAATG+TGG - chr1.2:46977739-46977758 MS.gene065301:intron 25.0%
! TTAGAGATAAAATTGTCTTG+AGG + chr1.2:46979697-46979716 None:intergenic 25.0%
! TTTGGATGTAAAAAATGAGT+GGG + chr1.2:46980638-46980657 None:intergenic 25.0%
!! AAATTTTTCTGTCCTTTCAA+TGG - chr1.2:46978175-46978194 MS.gene065301:intron 25.0%
!! ATGTCATTTGTATAGACTTT+GGG - chr1.2:46979926-46979945 MS.gene065301:intron 25.0%
!! GAGATATTTCCTTGATTTTA+GGG + chr1.2:46979621-46979640 None:intergenic 25.0%
!! GATGAAAGACAAAAATGTTT+TGG - chr1.2:46980690-46980709 MS.gene065301:intron 25.0%
!! TGAGATATTTCCTTGATTTT+AGG + chr1.2:46979622-46979641 None:intergenic 25.0%
!!! TCCTTTTTTTTAGAGTGTTT+TGG - chr1.2:46980057-46980076 MS.gene065301:intron 25.0%
!!! TTATTTTTTTCCCCACAAAA+CGG - chr1.2:46977684-46977703 MS.gene065301:intron 25.0%
AATATTGTTCGCCCATTTAT+TGG - chr1.2:46980211-46980230 MS.gene065301:intron 30.0%
AGTTACGTAATTAGGTGATA+AGG - chr1.2:46979859-46979878 MS.gene065301:intron 30.0%
ATATTGTTCGCCCATTTATT+GGG - chr1.2:46980212-46980231 MS.gene065301:intron 30.0%
ATCAAACAAACATACTCTTG+GGG + chr1.2:46980332-46980351 None:intergenic 30.0%
ATGAAAAGAGTGAAAGTTCA+TGG + chr1.2:46977262-46977281 None:intergenic 30.0%
CTAGTTACAAACAGATGTTA+TGG + chr1.2:46978288-46978307 None:intergenic 30.0%
CTTTGGATGTAAAAAATGAG+TGG + chr1.2:46980639-46980658 None:intergenic 30.0%
GACCTATCAAATGTAAACTT+TGG + chr1.2:46979759-46979778 None:intergenic 30.0%
GAGTTAGTAGTTACGTAATT+AGG - chr1.2:46979851-46979870 MS.gene065301:CDS 30.0%
GTTACGTAATTAGGTGATAA+GGG - chr1.2:46979860-46979879 MS.gene065301:intron 30.0%
TCATAAAGTTGAAACTGACT+TGG - chr1.2:46978657-46978676 MS.gene065301:intron 30.0%
TCATTTGTCTCTAAACTTAC+TGG + chr1.2:46978408-46978427 None:intergenic 30.0%
TGCCAAAGTTTACATTTGAT+AGG - chr1.2:46979754-46979773 MS.gene065301:intron 30.0%
TGTGGGGAAAAAAATAAGAA+AGG + chr1.2:46977681-46977700 None:intergenic 30.0%
TTTATACTCGATTTCTGCAA+AGG - chr1.2:46980592-46980611 MS.gene065301:CDS 30.0%
! AGGTATAGATACATTTTCAC+TGG - chr1.2:46980612-46980631 MS.gene065301:intron 30.0%
! ATATCTCAACTGACTCTATT+TGG - chr1.2:46979634-46979653 MS.gene065301:intron 30.0%
! CATGTCATTTGTATAGACTT+TGG - chr1.2:46979925-46979944 MS.gene065301:intron 30.0%
! GCCAAAACACTCTAAAAAAA+AGG + chr1.2:46980061-46980080 None:intergenic 30.0%
! TATCTCAACTGACTCTATTT+GGG - chr1.2:46979635-46979654 MS.gene065301:intron 30.0%
! TTAGCTTATTTCTCTTGTGA+AGG - chr1.2:46978597-46978616 MS.gene065301:intron 30.0%
!! AACTTGATTTTGGTCTATTC+TGG - chr1.2:46979107-46979126 MS.gene065301:intron 30.0%
!! GGTATATTTCTGGAAATTTC+TGG + chr1.2:46978557-46978576 None:intergenic 30.0%
!! TTTAATTCTGATTTGGGCTT+AGG - chr1.2:46978062-46978081 MS.gene065301:intron 30.0%
!!! AACAATCGGAATGTTTTAGT+TGG - chr1.2:46977800-46977819 MS.gene065301:intron 30.0%
!!! AATGTTTTAGTTGGTGGATA+AGG - chr1.2:46977809-46977828 MS.gene065301:intron 30.0%
AAAATCCTGCGGTATATTTC+TGG + chr1.2:46978567-46978586 None:intergenic 35.0%
AATGCAGAGGATTTGATTCT+TGG + chr1.2:46979278-46979297 None:intergenic 35.0%
AATGTTGTGTTTACGAGGAT+TGG + chr1.2:46979015-46979034 None:intergenic 35.0%
AATTTCCAGAAATATACCGC+AGG - chr1.2:46978559-46978578 MS.gene065301:intron 35.0%
ACCTTCTGCAATCTAACAAT+CGG - chr1.2:46977786-46977805 MS.gene065301:intron 35.0%
AGACCAAAATCAAGTTTACG+AGG + chr1.2:46979103-46979122 None:intergenic 35.0%
ATAGCAATGTCATGTCTTCT+AGG + chr1.2:46979044-46979063 None:intergenic 35.0%
ATGCAGAGGATTTGATTCTT+GGG + chr1.2:46979277-46979296 None:intergenic 35.0%
CATCGTGACTAATCTCAAAA+TGG - chr1.2:46977415-46977434 MS.gene065301:intron 35.0%
CATGAATCATTTCGTCAACA+GGG - chr1.2:46979989-46980008 MS.gene065301:intron 35.0%
CCTTCTACATAGAGTTCAAA+AGG + chr1.2:46979073-46979092 None:intergenic 35.0%
CTATTATAAAAGAGAGGGCA+GGG + chr1.2:46979592-46979611 None:intergenic 35.0%
CTTAAAAATGATCTGGTAGC+AGG + chr1.2:46979183-46979202 None:intergenic 35.0%
GGTCAGTTTATGTTGTTAAG+AGG - chr1.2:46979775-46979794 MS.gene065301:intron 35.0%
GGTGGAATAACAGAAATGAA+AGG - chr1.2:46979461-46979480 MS.gene065301:intron 35.0%
GTAAAGCTCTCCCAATAAAT+GGG + chr1.2:46980225-46980244 None:intergenic 35.0%
GTGCAAAGGACATAAAAGTT+TGG - chr1.2:46979239-46979258 MS.gene065301:intron 35.0%
TATTATAAAAGAGAGGGCAG+GGG + chr1.2:46979591-46979610 None:intergenic 35.0%
TCTATTATAAAAGAGAGGGC+AGG + chr1.2:46979593-46979612 None:intergenic 35.0%
TCTTATAGGACTCTGTGTTT+AGG + chr1.2:46979667-46979686 None:intergenic 35.0%
TGCATGAAAACCATTCCATT+TGG + chr1.2:46980774-46980793 None:intergenic 35.0%
TGTAAAGCTCTCCCAATAAA+TGG + chr1.2:46980226-46980245 None:intergenic 35.0%
! AAAGTCTCGAATCTTTTTGG+CGG - chr1.2:46978714-46978733 MS.gene065301:intron 35.0%
! ACTTTCACTCTTTTCATCAG+AGG - chr1.2:46977265-46977284 MS.gene065301:CDS 35.0%
! AGATAAGTTGACACAAGTGT+TGG - chr1.2:46978306-46978325 MS.gene065301:intron 35.0%
! CAATTGTAAACCGTTTTGTG+GGG + chr1.2:46977697-46977716 None:intergenic 35.0%
! CCTTTTGAACTCTATGTAGA+AGG - chr1.2:46979070-46979089 MS.gene065301:intron 35.0%
! GCAATTGTAAACCGTTTTGT+GGG + chr1.2:46977698-46977717 None:intergenic 35.0%
! GGTAAAGTCTCGAATCTTTT+TGG - chr1.2:46978711-46978730 MS.gene065301:intron 35.0%
! TGAGAAAACAAAAGGTGCAA+AGG - chr1.2:46979225-46979244 MS.gene065301:intron 35.0%
! TGCAATTGTAAACCGTTTTG+TGG + chr1.2:46977699-46977718 None:intergenic 35.0%
! TTATTTCTCTTGTGAAGGTG+AGG - chr1.2:46978602-46978621 MS.gene065301:intron 35.0%
!! AATGATAATGGTGAGCTCAA+AGG - chr1.2:46979427-46979446 MS.gene065301:intron 35.0%
!! ATTTTGACATGACAAGACAG+TGG + chr1.2:46980479-46980498 None:intergenic 35.0%
!! ATTTTGGTCTATTCTGGAAG+AGG - chr1.2:46979113-46979132 MS.gene065301:intron 35.0%
!! CTCTAAAAAAAAGTGTCGCT+AGG - chr1.2:46979711-46979730 MS.gene065301:intron 35.0%
!! GGATAAATGTGGTTGATTTG+TGG - chr1.2:46977750-46977769 MS.gene065301:intron 35.0%
!! GTATAACTTGTTTGGCAAGA+AGG - chr1.2:46980836-46980855 MS.gene065301:intron 35.0%
!! TAAAGTTGAAACTGACTTGG+AGG - chr1.2:46978660-46978679 MS.gene065301:intron 35.0%
!!! AATCGGAATGTTTTAGTTGG+TGG - chr1.2:46977803-46977822 MS.gene065301:intron 35.0%
!!! ATTGGTTTTGCCATCCAAAA+AGG + chr1.2:46978997-46979016 None:intergenic 35.0%
ACACTTGAATAGAAGGCAAG+CGG - chr1.2:46978950-46978969 MS.gene065301:intron 40.0%
AGCATCAGAAGAAAATCCTG+CGG + chr1.2:46978578-46978597 None:intergenic 40.0%
AGCTCAAAGGCTCTACAATA+TGG - chr1.2:46979440-46979459 MS.gene065301:intron 40.0%
CAACATTCCTGATGCATACA+TGG - chr1.2:46978508-46978527 MS.gene065301:intron 40.0%
CCATGAATCATTTCGTCAAC+AGG - chr1.2:46979988-46980007 MS.gene065301:intron 40.0%
CCATGAATGTTGTGTTTACG+AGG + chr1.2:46979020-46979039 None:intergenic 40.0%
CCTCGTAAACACAACATTCA+TGG - chr1.2:46979017-46979036 MS.gene065301:intron 40.0%
CCTGTTGACGAAATGATTCA+TGG + chr1.2:46979991-46980010 None:intergenic 40.0%
CGCACATTGCAAAACAAAAG+TGG + chr1.2:46977963-46977982 None:intergenic 40.0%
CTTGAGGCTTGCATTCTTAT+AGG + chr1.2:46979681-46979700 None:intergenic 40.0%
GACGCTGCCTATAAATATCA+GGG - chr1.2:46979882-46979901 MS.gene065301:intron 40.0%
GCTTATGACTAGTAATCAGC+TGG - chr1.2:46978145-46978164 MS.gene065301:intron 40.0%
GGTACTTGAGTAACAATCTG+CGG + chr1.2:46977579-46977598 None:intergenic 40.0%
GTAACAATCTGCGGAGTAAA+TGG + chr1.2:46977570-46977589 None:intergenic 40.0%
TAGACTCTCCAAACACCAAA+AGG + chr1.2:46979952-46979971 None:intergenic 40.0%
TCAAAGGCTCTACAATATGG+TGG - chr1.2:46979443-46979462 MS.gene065301:intron 40.0%
TCCACATGTAGACCATTGAA+AGG + chr1.2:46978190-46978209 None:intergenic 40.0%
TCCGATTGTTAGATTGCAGA+AGG + chr1.2:46977790-46977809 None:intergenic 40.0%
TCCTTTCAATGGTCTACATG+TGG - chr1.2:46978186-46978205 MS.gene065301:intron 40.0%
TGACAATTGCAGGGAGATTT+CGG - chr1.2:46977543-46977562 MS.gene065301:intron 40.0%
TGGGGATCCCTGATATTTAT+AGG + chr1.2:46979892-46979911 None:intergenic 40.0%
TTACCAACAGTCATCCATTG+AGG + chr1.2:46977654-46977673 None:intergenic 40.0%
TTGAGGACATCAGAGATGTA+AGG - chr1.2:46978632-46978651 MS.gene065301:intron 40.0%
! ATTCTGGAAGAGGTAGGTTT+AGG - chr1.2:46979123-46979142 MS.gene065301:intron 40.0%
! CAGCCTCGTAAACTTGATTT+TGG - chr1.2:46979097-46979116 MS.gene065301:intron 40.0%
! TCTTTTCATCAGAGGAGGTA+TGG - chr1.2:46977273-46977292 MS.gene065301:CDS 40.0%
! TGTATAGACTTTGGGCCTTT+TGG - chr1.2:46979934-46979953 MS.gene065301:intron 40.0%
! TTCACTCTTTTCATCAGAGG+AGG - chr1.2:46977268-46977287 MS.gene065301:CDS 40.0%
! TTGTTACTCAAGTACCGCTT+TGG - chr1.2:46977583-46977602 MS.gene065301:intron 40.0%
!! ACATACGCTTTGCTGTTGAA+GGG + chr1.2:46977894-46977913 None:intergenic 40.0%
!! GCTTGTATTTGCTTTTGCAG+AGG - chr1.2:46980794-46980813 MS.gene065301:intron 40.0%
!! TAAGTTGACACAAGTGTTGG+AGG - chr1.2:46978309-46978328 MS.gene065301:intron 40.0%
ACATGATAGATCGCTCTCTG+GGG + chr1.2:46979910-46979929 None:intergenic 45.0%
AGAGCAATTGCCAACCAAAG+CGG + chr1.2:46977600-46977619 None:intergenic 45.0%
AGAGTATGAACACTCCTACC+AGG - chr1.2:46979491-46979510 MS.gene065301:CDS 45.0%
ATTCTGGATGCAGAATGCAG+AGG + chr1.2:46979291-46979310 None:intergenic 45.0%
CAAATGCTCCGACATCTCTT+GGG + chr1.2:46978347-46978366 None:intergenic 45.0%
CAACAGTCATCCATTGAGGA+GGG + chr1.2:46977650-46977669 None:intergenic 45.0%
CACTTGAATAGAAGGCAAGC+GGG - chr1.2:46978951-46978970 MS.gene065301:intron 45.0%
CTTACAGAGATGAGACAGAG+AGG + chr1.2:46977443-46977462 None:intergenic 45.0%
GAACTCACCATGTATGCATC+AGG + chr1.2:46978518-46978537 None:intergenic 45.0%
GAAGGTGAGGTCTCTAATTG+AGG - chr1.2:46978615-46978634 MS.gene065301:intron 45.0%
GACATGATAGATCGCTCTCT+GGG + chr1.2:46979911-46979930 None:intergenic 45.0%
GAGTATGAACACTCCTACCA+GGG - chr1.2:46979492-46979511 MS.gene065301:CDS 45.0%
GGACGCTGCCTATAAATATC+AGG - chr1.2:46979881-46979900 MS.gene065301:intron 45.0%
GGAGAGATTTCTCTCTGGTT+AGG + chr1.2:46979527-46979546 None:intergenic 45.0%
GGCTGGAAAATACATGCTGT+TGG - chr1.2:46980097-46980116 MS.gene065301:intron 45.0%
GGTTAGGAGAGATTTCTCTC+TGG + chr1.2:46979532-46979551 None:intergenic 45.0%
GTACAGTGGTTGTGAATGTC+TGG - chr1.2:46978736-46978755 MS.gene065301:intron 45.0%
GTCTATCCGAAACTCTCTCT+GGG + chr1.2:46980288-46980307 None:intergenic 45.0%
TGACATGATAGATCGCTCTC+TGG + chr1.2:46979912-46979931 None:intergenic 45.0%
TGCCATCCAAAAAGGTCTAG+CGG + chr1.2:46978989-46979008 None:intergenic 45.0%
TGGTCGTACAATAGCTGTGA+AGG - chr1.2:46978690-46978709 MS.gene065301:intron 45.0%
TTTGGTTGGCAATTGCTCTC+AGG - chr1.2:46977601-46977620 MS.gene065301:intron 45.0%
! AACTGACTCTATTTGGGCCA+AGG - chr1.2:46979641-46979660 MS.gene065301:intron 45.0%
! TACTCAAGTACCGCTTTGGT+TGG - chr1.2:46977587-46977606 MS.gene065301:intron 45.0%
! TGGTCTATTCTGGAAGAGGT+AGG - chr1.2:46979117-46979136 MS.gene065301:intron 45.0%
! TTGGCGTGTGACGGAAAATT+TGG - chr1.2:46980076-46980095 MS.gene065301:intron 45.0%
!! AACATACTCTTGGGGCATTG+TGG + chr1.2:46980324-46980343 None:intergenic 45.0%
!! ACATACTCTTGGGGCATTGT+GGG + chr1.2:46980323-46980342 None:intergenic 45.0%
!! ACTGACTTGGAGGCATTCAA+TGG - chr1.2:46978670-46978689 MS.gene065301:intron 45.0%
!! GAATCTTTTTGGCGGTACAG+TGG - chr1.2:46978722-46978741 MS.gene065301:intron 45.0%
!! GACATACGCTTTGCTGTTGA+AGG + chr1.2:46977895-46977914 None:intergenic 45.0%
!! TGGTAGGAGTGTTCATACTC+TGG + chr1.2:46979492-46979511 None:intergenic 45.0%
!!! ACTTTGGGCCTTTTGGTGTT+TGG - chr1.2:46979941-46979960 MS.gene065301:intron 45.0%
!!! TAGAGTGTTTTGGCGTGTGA+CGG - chr1.2:46980067-46980086 MS.gene065301:intron 45.0%
AGGACTCTGTGTTTAGGCCT+TGG + chr1.2:46979661-46979680 None:intergenic 50.0%
CATCCATTGAGGAGGGACAA+TGG + chr1.2:46977643-46977662 None:intergenic 50.0%
CATCGCTGAAGACGAGATTG+TGG - chr1.2:46977391-46977410 MS.gene065301:intron 50.0%
CCAACAGTCATCCATTGAGG+AGG + chr1.2:46977651-46977670 None:intergenic 50.0%
CCTCCTCAATGGATGACTGT+TGG - chr1.2:46977648-46977667 MS.gene065301:intron 50.0%
CGTCTATCCGAAACTCTCTC+TGG + chr1.2:46980289-46980308 None:intergenic 50.0%
GCAAATGCTCCGACATCTCT+TGG + chr1.2:46978348-46978367 None:intergenic 50.0%
GCAATTGCTCTCAGGAAGAG+AGG - chr1.2:46977609-46977628 MS.gene065301:intron 50.0%
GCGAAACTCCCAAGAGATGT+CGG - chr1.2:46978336-46978355 MS.gene065301:intron 50.0%
! CGTGTGACGGAAAATTTGGC+TGG - chr1.2:46980080-46980099 MS.gene065301:intron 50.0%
CCCAGACCCAGAGAGAGTTT+CGG - chr1.2:46980279-46980298 MS.gene065301:intron 55.0%
CCGAAACTCTCTCTGGGTCT+GGG + chr1.2:46980282-46980301 None:intergenic 55.0%
GCTCCATTGTCCCTCCTCAA+TGG - chr1.2:46977637-46977656 MS.gene065301:intron 55.0%
TCCGAAACTCTCTCTGGGTC+TGG + chr1.2:46980283-46980302 None:intergenic 55.0%
TCTGGGTCTGGGTTCAAGTC+TGG + chr1.2:46980271-46980290 None:intergenic 55.0%
! CGCCGCTAGACCTTTTTGGA+TGG - chr1.2:46978984-46979003 MS.gene065301:intron 55.0%
! GTTGCGCCGCTAGACCTTTT+TGG - chr1.2:46978980-46978999 MS.gene065301:intron 55.0%
!! TAGGAGCATTGTGCCCTGGT+AGG + chr1.2:46979508-46979527 None:intergenic 55.0%
!! TGGTTAGGAGCATTGTGCCC+TGG + chr1.2:46979512-46979531 None:intergenic 55.0%
AGAGGGCAGGGGAGTGAGTA+AGG + chr1.2:46979580-46979599 None:intergenic 60.0%
!! GGGGCATTGTGGGCAACTTG+TGG + chr1.2:46980313-46980332 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 46977242 46980941 46977242 ID=MS.gene065301
chr1.2 mRNA 46977242 46980941 46977242 ID=MS.gene065301.t1;Parent=MS.gene065301
chr1.2 exon 46980894 46980941 46980894 ID=MS.gene065301.t1.exon1;Parent=MS.gene065301.t1
chr1.2 CDS 46980894 46980941 46980894 ID=cds.MS.gene065301.t1;Parent=MS.gene065301.t1
chr1.2 exon 46980726 46980800 46980726 ID=MS.gene065301.t1.exon2;Parent=MS.gene065301.t1
chr1.2 CDS 46980726 46980800 46980726 ID=cds.MS.gene065301.t1;Parent=MS.gene065301.t1
chr1.2 exon 46980514 46980628 46980514 ID=MS.gene065301.t1.exon3;Parent=MS.gene065301.t1
chr1.2 CDS 46980514 46980628 46980514 ID=cds.MS.gene065301.t1;Parent=MS.gene065301.t1
chr1.2 exon 46979776 46979875 46979776 ID=MS.gene065301.t1.exon4;Parent=MS.gene065301.t1
chr1.2 CDS 46979776 46979875 46979776 ID=cds.MS.gene065301.t1;Parent=MS.gene065301.t1
chr1.2 exon 46979654 46979684 46979654 ID=MS.gene065301.t1.exon5;Parent=MS.gene065301.t1
chr1.2 CDS 46979654 46979684 46979654 ID=cds.MS.gene065301.t1;Parent=MS.gene065301.t1
chr1.2 exon 46979472 46979564 46979472 ID=MS.gene065301.t1.exon6;Parent=MS.gene065301.t1
chr1.2 CDS 46979472 46979564 46979472 ID=cds.MS.gene065301.t1;Parent=MS.gene065301.t1
chr1.2 exon 46977847 46977999 46977847 ID=MS.gene065301.t1.exon7;Parent=MS.gene065301.t1
chr1.2 CDS 46977847 46977999 46977847 ID=cds.MS.gene065301.t1;Parent=MS.gene065301.t1
chr1.2 exon 46977242 46977388 46977242 ID=MS.gene065301.t1.exon8;Parent=MS.gene065301.t1
chr1.2 CDS 46977242 46977388 46977242 ID=cds.MS.gene065301.t1;Parent=MS.gene065301.t1
Gene Sequence

>MS.gene065301

ATGGCTGGGCAATCGAACCATGAACTTTCACTCTTTTCATCAGAGGAGGTATGGATGAATGCGATAATTGCCATATACAACTATGTTGTTATTCACATTGTTTCAGATTATTAATATATTAATTTTTTTTTTCTCAACTAGCTTGAGTTCATCGCTGAAGACGAGATTGTGGACATCGTGACTAATCTCAAAATGGATCCTCTCTGTCTCATCTCTGTAAGCAATTACTCATATCTCAAACACATCAACATTAGTTCTTATTTAGACACACATTTGTATTATGTTATTTAGTTTTTTTTAATGACAATTGCAGGGAGATTTCGGTCCATTTACTCCGCAGATTGTTACTCAAGTACCGCTTTGGTTGGCAATTGCTCTCAGGAAGAGAGGCAAGTGCTCCATTGTCCCTCCTCAATGGATGACTGTTGGTAAGCTTCCTTTCTTATTTTTTTCCCCACAAAACGGTTTACAATTGCAGTTTTTGTTACATAATGTTGTGAAAAATTAGGGATAAATGTGGTTGATTTGTGGAGTAGAATTTAAAACCTTCTGCAATCTAACAATCGGAATGTTTTAGTTGGTGGATAAGGCCATACATTTTTGTTCCAATTACTTTTATGTTATCTTTGTATTTATTTTTTATTCAAGGCCCTTCAACAGCAAAGCGTATGTCAGTATTGTCAAATTACTGCAATAAGCATAATTGGATTTTAAGAAACCACTTTTGTTTTGCAATGTGCGATTTAGTACAAAATGTTGTCAAATAACGTAGTGAAGTGAACAAATCGCTGTTTTCTAGATTGACAACACTAGCTTGTATTTTAATTCTGATTTGGGCTTAGGCATTCAACTATTTGTTGTTGTTTTTTGTTTAAAACCATTTGTTTTATATTTCTTGTTTTTGCTTATGACTAGTAATCAGCTGGTTCTTTTAAATTTTTCTGTCCTTTCAATGGTCTACATGTGGACAGTAGTACTTAGATGCTTTGATCTTTTTCCAGTTAAAAATTTGTTAGTTTGAGTAATAAAGACTTAATATTTCTCCATAACATCTGTTTGTAACTAGATAAGTTGACACAAGTGTTGGAGGCAGAGCGAAACTCCCAAGAGATGTCGGAGCATTTGCCATTTCATTATGTAGAAATTTCAAGACTTCTTTTTGACCAGTAAGTTTAGAGACAAATGATATCTTACTCTGTTTTTACTCAATATCTTATTTTACTATGTTAACTAATACCTTCTATTTTTTATTTTTAGTTCACGTGACAACATTCCTGATGCATACATGGTGAGTTCAAAGAAGTTTGACTTTCCAGAAATTTCCAGAAATATACCGCAGGATTTTCTTCTGATGCTTAGCTTATTTCTCTTGTGAAGGTGAGGTCTCTAATTGAGGACATCAGAGATGTAAGGTTTCATAAAGTTGAAACTGACTTGGAGGCATTCAATGGTCGTACAATAGCTGTGAAGGTAAAGTCTCGAATCTTTTTGGCGGTACAGTGGTTGTGAATGTCTGGATTGCAGCTGTGATTTGACTTTTTTTTTTATATATAAGGATTTAATTTTATTAGTGTTGTGTTTTTTATTTTCAGTTGCTTTTAAGATTTCAAACTAGTATAGTAATTACCCGTATTTGATTTGATTACAAAACATATTTCAAGTTAAACTATGTCACAATTAAATCTCCTTTATGTATAAACCATTATTTACACTTGAATAGAAGGCAAGCGGGCAACAAGTTGCGCCGCTAGACCTTTTTGGATGGCAAAACCAATCCTCGTAAACACAACATTCATGGACCTAGAAGACATGACATTGCTATGCATGACCTTTTGAACTCTATGTAGAAGGTCAACAGCCTCGTAAACTTGATTTTGGTCTATTCTGGAAGAGGTAGGTTTAGGTTCTGTAGTTAAATTGTCTCATCTCATTTATGCTCCTGCTACCAGATCATTTTTAAGAAAGTTCCACTGTAATTAAATATGAGAAAACAAAAGGTGCAAAGGACATAAAAGTTTGGTAATTAGCTTAGCCCAAGAATCAAATCCTCTGCATTCTGCATCCAGAATAGATTAGATTCTGAATCTTATAGTTTTATGCTCCATATCTCTTTCATATATTAGGACATTTTAAACTGAATGTGACAGAACTCGTAAAATCAAGATAATTAAACTGTATTTTATTGAATGATAATGGTGAGCTCAAAGGCTCTACAATATGGTGGAATAACAGAAATGAAAGGATAAACCAGAGTATGAACACTCCTACCAGGGCACAATGCTCCTAACCAGAGAGAAATCTCTCCTAACCATCTTTCTAACAATATTTCTCAATGCCTTACTCACTCCCCTGCCCTCTCTTTTATAATAGAAATATCCCTAAAATCAAGGAAATATCTCAACTGACTCTATTTGGGCCAAGGCCTAAACACAGAGTCCTATAAGAATGCAAGCCTCAAGACAATTTTATCTCTAAAAAAAAGTGTCGCTAGGTGATAGAGACATTGAGTTAATGCCAAAGTTTACATTTGATAGGTCAGTTTATGTTGTTAAGAGGTTAATCTGTTAATTATTTGTTAAGAGTTAGTTGTTTTTTTTTAACAAGGTTAAGAGTTAGTAGTTACGTAATTAGGTGATAAGGGACGCTGCCTATAAATATCAGGGATCCCCAGAGAGCGATCTATCATGTCATTTGTATAGACTTTGGGCCTTTTGGTGTTTGGAGAGTCTAGAGTCTCTCGAATACTCCATGAATCATTTCGTCAACAGGGATCTTCTCTAATTTCTTGATAATTTCCTTATTTTTTAAGTTCTATTCCTTTTTTTTAGAGTGTTTTGGCGTGTGACGGAAAATTTGGCTGGAAAATACATGCTGTTGGTCTTGTTACATATCTGTTCATTTCTCTGCTGACATTTTACATCATAATTCTTGGTATGTTGCAGATTAAAAATTTATCTGCAATGGAAGTGAATATTGTTCGCCCATTTATTGGGAGAGCTTTACAAGCATTTTACAAGCATGATAGTCCAGACTTGAACCCAGACCCAGAGAGAGTTTCGGATAGACGACCACAAGTTGCCCACAATGCCCCAAGAGTATGTTTGTTTGATATACTCATGTTTCATGCACTAAAAAAATTAAACATTTACTTATCTGCTATGTACTTTTTGTGCGAGTTTAATTAACTATACGTCAATAGCATAAACATGTTTTACAGAGACGTTCAATCATATTCCACTGTCTTGTCATGTCAAAATGTGTTCTTTCAGAAAATGTTAACTAAATTGTGATTATATAGATGTCTACATGAAACTTGTTCACACTATGATTGTAAAGTTATTTAAATTTTATTTATACTCGATTTCTGCAAAGGTATAGATACATTTTCACTGGCCCACTCATTTTTTACATCCAAAGATATAAAAATTCAATATAATTAATTTTAAATGATGAAAGACAAAAATGTTTTGGTCTATCATATATATATATATATATATATATATATATATATATATATATATATATTCTTCCAAATGGAATGGTTTTCATGCAGCTTGTATTTGCTTTTGCAGAGGCAACTACGAAGATAATTATGTATAACTTGTTTGGCAAGAAGGCTTCTAATTTGCTAAATCTGACTTGCTTCAAAGTTACATCTGTACTTAAGAAGTTCATCGACTCCCATGTACTTCTCAAATAG
Protein sequence

>MS.gene065301.t1

MAGQSNHELSLFSSEELEFIAEDEIVDIVTNLKMDPLCLISGDFGPFTPQIVTQVPLWLAIALRKRGKCSIVPPQWMTVDKLTQVLEAERNSQEMSEHLPFHYVEISRLLFDHSRDNIPDAYMVRSLIEDIRDVRFHKVETDLEAFNGRTIAVKIKNLSAMEVNIVRPFIGRALQAFYKHDSPDLNPDPERVSDRRPQVAHNAPRLVFAFAEATTKIIMYNLFGKKASNLLNLTCFKVTSVLKKFIDSHVLLK