AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025347

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025347.t1 MTR_2g044210 99.119 227 2 0 1 227 1 227 2.35e-164 452
MS.gene025347.t1 MTR_2g044210 91.667 216 15 1 1 216 1 213 2.88e-141 395
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025347.t1 AT5G49010 58.590 227 87 2 1 227 1 220 5.92e-87 256
MS.gene025347.t1 AT5G49010 60.345 174 65 1 1 174 1 170 1.25e-66 204

Find 43 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGAACCTTGTTTCTATTA+AGG 0.244460 2.2:+42732914 MS.gene025347:CDS
AACTTTGGAACAGACTTTCT+AGG 0.280314 2.2:+42733369 MS.gene025347:CDS
CAAAGGACAGATTGAATTGA+TGG 0.395331 2.2:+42732568 MS.gene025347:CDS
CAAAACAAACGTGTCTAGTC+GGG 0.413755 2.2:-42735017 None:intergenic
TGCTCTCTAGATGTTCCTTC+AGG 0.422302 2.2:-42733559 None:intergenic
AACATCAGTGGTTGAAATCA+AGG 0.435422 2.2:-42732465 None:intergenic
CAAATGAAGTAAAGGACATC+AGG 0.440202 2.2:-42735202 None:intergenic
GATAATTGCCTGTCATCAAC+AGG 0.456044 2.2:-42735085 None:intergenic
GATTCAAAACGAAGAATCTC+GGG 0.462194 2.2:-42732527 None:intergenic
TTCTGTATCTCTGTATCAGA+TGG 0.469476 2.2:+42732885 MS.gene025347:CDS
ATCCATGGGTTCAAGATCTA+CGG 0.481878 2.2:+42732406 None:intergenic
ACTTTGGAACAGACTTTCTA+GGG 0.492617 2.2:+42733370 MS.gene025347:CDS
AACGTGTCTAGTCGGGGTGC+TGG 0.493562 2.2:-42735010 None:intergenic
AGATTCAAAACGAAGAATCT+CGG 0.501966 2.2:-42732528 None:intergenic
GCGAGCATGGCGTAACGAAA+AGG 0.511669 2.2:+42732499 MS.gene025347:CDS
ATAAAACCACTTGTAGAGAG+TGG 0.514618 2.2:+42735235 MS.gene025347:CDS
GATTTGATCAACCGCGTCAA+AGG 0.524234 2.2:+42732551 MS.gene025347:CDS
TTCCGTAGATCTTGAACCCA+TGG 0.527155 2.2:-42732408 None:intergenic
TTACCTTCAGGATGCGGATG+CGG 0.527448 2.2:-42732945 None:intergenic
CAGGTCGAAGCTGTTTGAGA+TGG 0.531476 2.2:+42735177 MS.gene025347:intron
ATTTGTAACAAATGAAGTAA+AGG 0.537264 2.2:-42735210 None:intergenic
GACTGATTACCTTCAGGATG+CGG 0.542811 2.2:-42732951 None:intergenic
ACAGGAATTCAACTTGAAGA+TGG 0.546681 2.2:+42735061 MS.gene025347:CDS
ACAAAACAAACGTGTCTAGT+CGG 0.547750 2.2:-42735018 None:intergenic
CATTAGTGAAGAGGATGACA+TGG 0.553868 2.2:+42733634 MS.gene025347:CDS
TTGAGCTTCTCAAGCGAGCA+TGG 0.560140 2.2:+42732486 MS.gene025347:CDS
ACAATCTGTCATTAGTGAAG+AGG 0.570258 2.2:+42733625 MS.gene025347:CDS
AAGATGGACCTGTTGATGAC+AGG 0.572063 2.2:+42735077 MS.gene025347:CDS
TCTCCGCATCCGCATCCTGA+AGG 0.576234 2.2:+42732942 MS.gene025347:CDS
TAAAACCACTTGTAGAGAGT+GGG 0.580787 2.2:+42735236 MS.gene025347:CDS
TGATACAGAGATACAGAAAG+AGG 0.584753 2.2:-42732880 None:intergenic
AGAAGTAAAGAATATCTCAC+AGG 0.604102 2.2:+42735043 MS.gene025347:CDS
CTTGAGAAGCTCAACATCAG+TGG 0.611012 2.2:-42732477 None:intergenic
GATACAGAGATACAGAAAGA+GGG 0.651435 2.2:-42732879 None:intergenic
CAGGTGCACAGATGACCTGA+AGG 0.652118 2.2:+42733544 MS.gene025347:intron
GTGGAAGAGAAATTGTCTGT+TGG 0.657885 2.2:+42732850 MS.gene025347:CDS
AAAACAAACGTGTCTAGTCG+GGG 0.662409 2.2:-42735016 None:intergenic
ATGGGTTCAAGATCTACGGA+AGG 0.674495 2.2:+42732410 MS.gene025347:CDS
ATTCAAAACGAAGAATCTCG+GGG 0.677114 2.2:-42732526 None:intergenic
ATTCAATCTGTCCTTTGACG+CGG 0.682411 2.2:-42732562 None:intergenic
ATTAGTGAAGAGGATGACAT+GGG 0.687742 2.2:+42733635 MS.gene025347:CDS
ATATGACCTTAATAGAAACA+AGG 0.692807 2.2:-42732920 None:intergenic
TTCAAAACGAAGAATCTCGG+GGG 0.737687 2.2:-42732525 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTAAATTTCTTATTGT+CGG + chr2.2:42732729-42732748 MS.gene025347:intron 15.0%
!! AATCTAGTTAAAGATTAATA+AGG - chr2.2:42733118-42733137 None:intergenic 15.0%
!! AAAGTGATAGGATTATTTAT+GGG + chr2.2:42732997-42733016 MS.gene025347:intron 20.0%
!! AAATTGAAATTCATTTCAGT+TGG - chr2.2:42732649-42732668 None:intergenic 20.0%
!! AAGAAATAACAAAACCAAAA+AGG - chr2.2:42733682-42733701 None:intergenic 20.0%
!! AGAATCATTTAGTTAGTTAT+GGG + chr2.2:42734410-42734429 MS.gene025347:intron 20.0%
!! ATTTGTAACAAATGAAGTAA+AGG - chr2.2:42735213-42735232 None:intergenic 20.0%
!! CAAAGACATAACATATAAAA+AGG - chr2.2:42734924-42734943 None:intergenic 20.0%
!! CTAAAAAATTGAAAAGAGAA+GGG + chr2.2:42734197-42734216 MS.gene025347:intron 20.0%
!! TAAAAAATTGAAAAGAGAAG+GGG + chr2.2:42734198-42734217 MS.gene025347:intron 20.0%
!! TATGTTAAGAAACATAAGAA+GGG + chr2.2:42734291-42734310 MS.gene025347:intron 20.0%
!! TTACATAAAACCTATGATAT+AGG - chr2.2:42733204-42733223 None:intergenic 20.0%
!! TTATGTTAAGAAACATAAGA+AGG + chr2.2:42734290-42734309 MS.gene025347:intron 20.0%
!! TTATTAAATGTGAAGTAACT+AGG - chr2.2:42732799-42732818 None:intergenic 20.0%
!! TTGTTGAAATATTTGAATAC+TGG + chr2.2:42733240-42733259 MS.gene025347:intron 20.0%
!!! AAAATTTCTTGTTACCATTT+TGG + chr2.2:42733776-42733795 MS.gene025347:intron 20.0%
!!! ATGAAAGATTTTGAAATGAT+TGG + chr2.2:42733807-42733826 MS.gene025347:intron 20.0%
!!! CTAGATTGAAATTTGATTTT+CGG + chr2.2:42733131-42733150 MS.gene025347:intron 20.0%
!!! CTTTCTAACTTTTAAAAAAC+AGG - chr2.2:42735135-42735154 None:intergenic 20.0%
!!! GATTTTGATGTAATACTTTA+TGG + chr2.2:42734005-42734024 MS.gene025347:intron 20.0%
!!! GTTTTTTAATCTAGTTTTCT+GGG + chr2.2:42733519-42733538 MS.gene025347:intron 20.0%
!!! TAATGATTTGGATAGAAATA+TGG + chr2.2:42734688-42734707 MS.gene025347:intron 20.0%
!!! TGTTTTTTAATCTAGTTTTC+TGG + chr2.2:42733518-42733537 MS.gene025347:intron 20.0%
!!! TTTGGTTTTGTTATTTCTTA+TGG + chr2.2:42733683-42733702 MS.gene025347:intron 20.0%
! AAACAAAATGTCATTGTAGA+TGG - chr2.2:42734976-42734995 None:intergenic 25.0%
! AAATATTATCTCGCAAATAG+AGG - chr2.2:42733162-42733181 None:intergenic 25.0%
! AAGAAATCACAAAAAGTAGA+AGG - chr2.2:42732615-42732634 None:intergenic 25.0%
! AATCTAGTGAATATAGCAAT+AGG - chr2.2:42733274-42733293 None:intergenic 25.0%
! AGATCTAGAGATTAATGATT+TGG + chr2.2:42734676-42734695 MS.gene025347:intron 25.0%
! ATATGACCTTAATAGAAACA+AGG - chr2.2:42732923-42732942 None:intergenic 25.0%
! CAAAGTGATAGGATTATTTA+TGG + chr2.2:42732996-42733015 MS.gene025347:intron 25.0%
! CCTAAAAAATTGAAAAGAGA+AGG + chr2.2:42734196-42734215 MS.gene025347:intron 25.0%
! GAGAATCATTTAGTTAGTTA+TGG + chr2.2:42734409-42734428 MS.gene025347:intron 25.0%
! GTAAGGCAACAATAAATTAT+TGG - chr2.2:42733076-42733095 None:intergenic 25.0%
! TAGTTAAAGATTAATAAGGC+CGG - chr2.2:42733114-42733133 None:intergenic 25.0%
! TATGATATAGGTAGAAACTA+AGG - chr2.2:42733192-42733211 None:intergenic 25.0%
! TTACTGATAATAACTACAGT+TGG + chr2.2:42734529-42734548 MS.gene025347:intron 25.0%
! TTAGTTTCTACCTATATCAT+AGG + chr2.2:42733191-42733210 MS.gene025347:intron 25.0%
! TTATTATCAGTAAAGCTACA+AGG - chr2.2:42734522-42734541 None:intergenic 25.0%
! TTCGATCTGCAATATAAATA+TGG - chr2.2:42733318-42733337 None:intergenic 25.0%
! TTTCATATTACTTGCCAAAA+TGG - chr2.2:42733793-42733812 None:intergenic 25.0%
!! ATGAGACTTTTCAAATTTCT+AGG - chr2.2:42734164-42734183 None:intergenic 25.0%
!! CCTTCTCTTTTCAATTTTTT+AGG - chr2.2:42734199-42734218 None:intergenic 25.0%
!! TAATATGGTTTTTCATGGAA+AGG - chr2.2:42734814-42734833 None:intergenic 25.0%
!! TCATAAGACTGATAATTTTC+AGG - chr2.2:42733602-42733621 None:intergenic 25.0%
!!! ATAGAACCTTGTTTCTATTA+AGG + chr2.2:42732914-42732933 MS.gene025347:CDS 25.0%
!!! GAATTTCAATTTTGAAGACA+TGG + chr2.2:42732657-42732676 MS.gene025347:intron 25.0%
!!! GTTTTAAAAGATAGCAATGT+AGG + chr2.2:42733487-42733506 MS.gene025347:intron 25.0%
!!! TTCAATTTTTTAGGTTTGCA+TGG - chr2.2:42734190-42734209 None:intergenic 25.0%
!!! TTTAATTTGATTATGCTTGC+AGG + chr2.2:42735158-42735177 MS.gene025347:intron 25.0%
AAAAAGGACAGCATGATTTA+TGG - chr2.2:42734908-42734927 None:intergenic 30.0%
AACACAAATGGAAAAAAACG+AGG - chr2.2:42734849-42734868 None:intergenic 30.0%
AAGCAGTAATAAAGTTCGTT+AGG - chr2.2:42734479-42734498 None:intergenic 30.0%
ACACAAATGGAAAAAAACGA+GGG - chr2.2:42734848-42734867 None:intergenic 30.0%
AGAAGTAAAGAATATCTCAC+AGG + chr2.2:42735043-42735062 MS.gene025347:CDS 30.0%
AGATTCAAAACGAAGAATCT+CGG - chr2.2:42732531-42732550 None:intergenic 30.0%
CATGAAAAACCATATTAGAG+AGG + chr2.2:42734817-42734836 MS.gene025347:intron 30.0%
CATTTAGCATCATCCTAATT+TGG - chr2.2:42733986-42734005 None:intergenic 30.0%
CCAAAAAGGACAACTAATAA+TGG - chr2.2:42733668-42733687 None:intergenic 30.0%
GATGTAATACTTTATGGTGA+TGG + chr2.2:42734011-42734030 MS.gene025347:intron 30.0%
TATGTTATGTCTTTGACACT+AGG + chr2.2:42734929-42734948 MS.gene025347:intron 30.0%
TCAAAATTAAGCACAACCAA+AGG - chr2.2:42734077-42734096 None:intergenic 30.0%
TCAGAATCTTTCATTAACCA+TGG + chr2.2:42734135-42734154 MS.gene025347:intron 30.0%
TGAAGGAAAAAATGTTGATG+TGG + chr2.2:42734314-42734333 MS.gene025347:intron 30.0%
TTGGATATGAAGAAGATAGT+GGG + chr2.2:42734378-42734397 MS.gene025347:intron 30.0%
TTTGGATATGAAGAAGATAG+TGG + chr2.2:42734377-42734396 MS.gene025347:intron 30.0%
! AAACTTTTCACTGAAAGGTA+AGG + chr2.2:42733398-42733417 MS.gene025347:intron 30.0%
! AGCTATCTTTTACAACACAT+TGG - chr2.2:42733428-42733447 None:intergenic 30.0%
! ATGAGAAACTTTTCACTGAA+AGG + chr2.2:42733393-42733412 MS.gene025347:CDS 30.0%
! ATGTGTTTTTCTCCTTTTAC+AGG + chr2.2:42732819-42732838 MS.gene025347:intron 30.0%
! CCATTATTAGTTGTCCTTTT+TGG + chr2.2:42733665-42733684 MS.gene025347:intron 30.0%
! CTCTCTAATATGGTTTTTCA+TGG - chr2.2:42734819-42734838 None:intergenic 30.0%
! GTAACCTTTCATTGATGTTT+TGG + chr2.2:42732766-42732785 MS.gene025347:intron 30.0%
! TCCAAAGTTCTTGAGTTTTT+CGG - chr2.2:42733358-42733377 None:intergenic 30.0%
! TTCAAATTTCTAGGTTTCCA+TGG - chr2.2:42734155-42734174 None:intergenic 30.0%
! TTTTCTCTCTTGCTAATTGA+GGG + chr2.2:42734867-42734886 MS.gene025347:intron 30.0%
!! TTTTGATAGCTAATCCTCAA+AGG + chr2.2:42734100-42734119 MS.gene025347:intron 30.0%
!!! GTTTCTCTTCTAGTTTTTCT+AGG - chr2.2:42734647-42734666 None:intergenic 30.0%
AAAGGTCATTAAGTGCTTAC+AGG - chr2.2:42734059-42734078 None:intergenic 35.0%
AACATCAGTGGTTGAAATCA+AGG - chr2.2:42732468-42732487 None:intergenic 35.0%
AAGAAACATAAGAAGGGTGA+AGG + chr2.2:42734297-42734316 MS.gene025347:intron 35.0%
AAGGTCATTAAGTGCTTACA+GGG - chr2.2:42734058-42734077 None:intergenic 35.0%
ACAAAACAAACGTGTCTAGT+CGG - chr2.2:42735021-42735040 None:intergenic 35.0%
ACAATCTGTCATTAGTGAAG+AGG + chr2.2:42733625-42733644 MS.gene025347:CDS 35.0%
ACAGGAATTCAACTTGAAGA+TGG + chr2.2:42735061-42735080 MS.gene025347:CDS 35.0%
ACCACCAAAACATCAATGAA+AGG - chr2.2:42732773-42732792 None:intergenic 35.0%
ACTTTATTACTGCTTGCAAC+TGG + chr2.2:42734484-42734503 MS.gene025347:intron 35.0%
AGATGGATCATTTCTGTGAT+GGG + chr2.2:42734344-42734363 MS.gene025347:intron 35.0%
AGCAAGAGAGAAAACACAAA+TGG - chr2.2:42734861-42734880 None:intergenic 35.0%
AGGAGAAAAAAATTCATGCG+AGG - chr2.2:42735115-42735134 None:intergenic 35.0%
ATAAAACCACTTGTAGAGAG+TGG + chr2.2:42735235-42735254 MS.gene025347:CDS 35.0%
ATTAGTGAAGAGGATGACAT+GGG + chr2.2:42733635-42733654 MS.gene025347:CDS 35.0%
ATTCAAAACGAAGAATCTCG+GGG - chr2.2:42732529-42732548 None:intergenic 35.0%
CAAAGGACAGATTGAATTGA+TGG + chr2.2:42732568-42732587 MS.gene025347:CDS 35.0%
CAAATGAAGTAAAGGACATC+AGG - chr2.2:42735205-42735224 None:intergenic 35.0%
CATCCTTCCATTACCAAATT+AGG + chr2.2:42733970-42733989 MS.gene025347:intron 35.0%
GAATGTAAAAGTGACCTTTG+AGG - chr2.2:42734117-42734136 None:intergenic 35.0%
GATACAGAGATACAGAAAGA+GGG - chr2.2:42732882-42732901 None:intergenic 35.0%
GATATAGGTAGAAACTAAGG+TGG - chr2.2:42733189-42733208 None:intergenic 35.0%
GATTCAAAACGAAGAATCTC+GGG - chr2.2:42732530-42732549 None:intergenic 35.0%
GCATCATCCTAATTTGGTAA+TGG - chr2.2:42733980-42733999 None:intergenic 35.0%
TAAAACCACTTGTAGAGAGT+GGG + chr2.2:42735236-42735255 MS.gene025347:CDS 35.0%
TCCGAAAAACTCAAGAACTT+TGG + chr2.2:42733354-42733373 MS.gene025347:CDS 35.0%
TGATACAGAGATACAGAAAG+AGG - chr2.2:42732883-42732902 None:intergenic 35.0%
TGGTATTTGATAGAACAGTC+TGG + chr2.2:42734708-42734727 MS.gene025347:intron 35.0%
TGTAAGCACTTAATGACCTT+TGG + chr2.2:42734058-42734077 MS.gene025347:intron 35.0%
TTAAAGTGCATGAATCCCTT+AGG + chr2.2:42733942-42733961 MS.gene025347:intron 35.0%
TTACCCTCAAGTATTAACAC+CGG + chr2.2:42733092-42733111 MS.gene025347:intron 35.0%
TTCTGTATCTCTGTATCAGA+TGG + chr2.2:42732885-42732904 MS.gene025347:CDS 35.0%
! GCTTTTACACTCAAAGTGAT+AGG + chr2.2:42732985-42733004 MS.gene025347:intron 35.0%
! GTTTTCTCTCTTGCTAATTG+AGG + chr2.2:42734866-42734885 MS.gene025347:intron 35.0%
!! AACTTTGGAACAGACTTTCT+AGG + chr2.2:42733369-42733388 MS.gene025347:CDS 35.0%
!! AAGAAGATAGTGGGATATCT+TGG + chr2.2:42734387-42734406 MS.gene025347:intron 35.0%
!! ACCTTTCATTGATGTTTTGG+TGG + chr2.2:42732769-42732788 MS.gene025347:intron 35.0%
!! ACTTTGGAACAGACTTTCTA+GGG + chr2.2:42733370-42733389 MS.gene025347:CDS 35.0%
!! CATCCTAATTTGGTAATGGA+AGG - chr2.2:42733976-42733995 None:intergenic 35.0%
!! GTGATGGGTTCTGTTATATT+TGG + chr2.2:42734359-42734378 MS.gene025347:intron 35.0%
AAAACAAACGTGTCTAGTCG+GGG - chr2.2:42735019-42735038 None:intergenic 40.0%
ACATAAACTCTCCACACTGT+AGG - chr2.2:42734276-42734295 None:intergenic 40.0%
AGATAGCAATGTAGGCTACT+TGG + chr2.2:42733495-42733514 MS.gene025347:intron 40.0%
AGTAAGGAGAGTAGATCAGA+TGG + chr2.2:42734589-42734608 MS.gene025347:intron 40.0%
AGTCAAATCACTAGAGGTAG+AGG + chr2.2:42734617-42734636 MS.gene025347:intron 40.0%
AGTCCTAGACAGACACAAAA+TGG - chr2.2:42733710-42733729 None:intergenic 40.0%
ATTCAATCTGTCCTTTGACG+CGG - chr2.2:42732565-42732584 None:intergenic 40.0%
CAAAACAAACGTGTCTAGTC+GGG - chr2.2:42735020-42735039 None:intergenic 40.0%
CATTAGTGAAGAGGATGACA+TGG + chr2.2:42733634-42733653 MS.gene025347:CDS 40.0%
CCACAGTTTCTTCCTGTAAA+AGG - chr2.2:42732834-42732853 None:intergenic 40.0%
GAGATGGATCATTTCTGTGA+TGG + chr2.2:42734343-42734362 MS.gene025347:intron 40.0%
GATAATTGCCTGTCATCAAC+AGG - chr2.2:42735088-42735107 None:intergenic 40.0%
GGTGTTAATACTTGAGGGTA+AGG - chr2.2:42733093-42733112 None:intergenic 40.0%
GTAGATGGTTCGTTTATGTC+AGG - chr2.2:42734961-42734980 None:intergenic 40.0%
GTGGAAGAGAAATTGTCTGT+TGG + chr2.2:42732850-42732869 MS.gene025347:CDS 40.0%
TCCTGAAGGTAATCAGTCAT+AGG + chr2.2:42732956-42732975 MS.gene025347:intron 40.0%
TCTCTTGCTAATTGAGGGTT+TGG + chr2.2:42734872-42734891 MS.gene025347:intron 40.0%
TTCAAAACGAAGAATCTCGG+GGG - chr2.2:42732528-42732547 None:intergenic 40.0%
! ATTTTCCCACTCTCTACAAG+TGG - chr2.2:42735244-42735263 None:intergenic 40.0%
! CATGTGCTAACTGCACATTT+TGG - chr2.2:42734241-42734260 None:intergenic 40.0%
! CCTTTTACAGGAAGAAACTG+TGG + chr2.2:42732831-42732850 MS.gene025347:intron 40.0%
!! GTTCTTGAGTTTTTCGGATG+TGG - chr2.2:42733352-42733371 None:intergenic 40.0%
!!! TAATCTAGTTTTCTGGGTGC+AGG + chr2.2:42733525-42733544 MS.gene025347:intron 40.0%
AAGGAGAGTAGATCAGATGG+AGG + chr2.2:42734592-42734611 MS.gene025347:intron 45.0%
ACTAAGTGGAGTTGGCTACA+TGG - chr2.2:42734745-42734764 None:intergenic 45.0%
AGGAGAGTAGATCAGATGGA+GGG + chr2.2:42734593-42734612 MS.gene025347:intron 45.0%
AGGGAGAAGCCTCTCTAATA+TGG - chr2.2:42734829-42734848 None:intergenic 45.0%
ATGGAAGGATGAACAGCCTA+AGG - chr2.2:42733961-42733980 None:intergenic 45.0%
ATGGGTTCAAGATCTACGGA+AGG + chr2.2:42732410-42732429 MS.gene025347:CDS 45.0%
ATGTAGCCAACTCCACTTAG+TGG + chr2.2:42734744-42734763 MS.gene025347:intron 45.0%
CAACTCCACTTAGTGGGATA+AGG + chr2.2:42734751-42734770 MS.gene025347:intron 45.0%
CTCGGAATAAGCTTAGCACT+GGG + chr2.2:42734448-42734467 MS.gene025347:intron 45.0%
CTTATCCCACTAAGTGGAGT+TGG - chr2.2:42734753-42734772 None:intergenic 45.0%
CTTGAGAAGCTCAACATCAG+TGG - chr2.2:42732480-42732499 None:intergenic 45.0%
GACTGATTACCTTCAGGATG+CGG - chr2.2:42732954-42732973 None:intergenic 45.0%
GATTTGATCAACCGCGTCAA+AGG + chr2.2:42732551-42732570 MS.gene025347:CDS 45.0%
GCCTATGACTGATTACCTTC+AGG - chr2.2:42732960-42732979 None:intergenic 45.0%
TCATGTAAAGAGAAGACCCG+CGG + chr2.2:42734559-42734578 MS.gene025347:intron 45.0%
TCTCCTTACTACAGAATCCG+CGG - chr2.2:42734579-42734598 None:intergenic 45.0%
TCTCGGAATAAGCTTAGCAC+TGG + chr2.2:42734447-42734466 MS.gene025347:intron 45.0%
TGCTCTCTAGATGTTCCTTC+AGG - chr2.2:42733562-42733581 None:intergenic 45.0%
TGGAAGGATGAACAGCCTAA+GGG - chr2.2:42733960-42733979 None:intergenic 45.0%
TGTAGCCAACTCCACTTAGT+GGG + chr2.2:42734745-42734764 MS.gene025347:intron 45.0%
! AAGGCCGGTGTTAATACTTG+AGG - chr2.2:42733099-42733118 None:intergenic 45.0%
! AAGGGGCTAAATGAGAGTGT+GGG + chr2.2:42734215-42734234 MS.gene025347:intron 45.0%
! AGGCCGGTGTTAATACTTGA+GGG - chr2.2:42733098-42733117 None:intergenic 45.0%
! CTAAGCCTTATCCCACTAAG+TGG - chr2.2:42734759-42734778 None:intergenic 45.0%
! CTCTCCACACTGTAGGTTTT+AGG - chr2.2:42734269-42734288 None:intergenic 45.0%
! GGACCATTTTGTGTCTGTCT+AGG + chr2.2:42733704-42733723 MS.gene025347:intron 45.0%
! TTGTGTCTGTCTAGGACTGT+AGG + chr2.2:42733712-42733731 MS.gene025347:intron 45.0%
!! AAGATGGACCTGTTGATGAC+AGG + chr2.2:42735077-42735096 MS.gene025347:CDS 45.0%
CAGGTCGAAGCTGTTTGAGA+TGG + chr2.2:42735177-42735196 MS.gene025347:intron 50.0%
CTCCTTACTACAGAATCCGC+GGG - chr2.2:42734578-42734597 None:intergenic 50.0%
GAAGGGGCTAAATGAGAGTG+TGG + chr2.2:42734214-42734233 MS.gene025347:intron 50.0%
GAGGGGAGTCAAATCACTAG+AGG + chr2.2:42734611-42734630 MS.gene025347:intron 50.0%
GCTGCCTAAAACCTACAGTG+TGG + chr2.2:42734262-42734281 MS.gene025347:intron 50.0%
GGAGAGTAGATCAGATGGAG+GGG + chr2.2:42734594-42734613 MS.gene025347:intron 50.0%
GTTGATGTGGCAGAGAGAGA+TGG + chr2.2:42734327-42734346 MS.gene025347:intron 50.0%
TTACCTTCAGGATGCGGATG+CGG - chr2.2:42732948-42732967 None:intergenic 50.0%
TTGAGCTTCTCAAGCGAGCA+TGG + chr2.2:42732486-42732505 MS.gene025347:CDS 50.0%
!! GGGAAGAGAGCATCTGTTCT+CGG + chr2.2:42734430-42734449 MS.gene025347:intron 50.0%
CAGGTGCACAGATGACCTGA+AGG + chr2.2:42733544-42733563 MS.gene025347:intron 55.0%
GACCCGCGGATTCTGTAGTA+AGG + chr2.2:42734573-42734592 MS.gene025347:intron 55.0%
GCGAGCATGGCGTAACGAAA+AGG + chr2.2:42732499-42732518 MS.gene025347:CDS 55.0%
AACGTGTCTAGTCGGGGTGC+TGG - chr2.2:42735013-42735032 None:intergenic 60.0%
TCTCCGCATCCGCATCCTGA+AGG + chr2.2:42732942-42732961 MS.gene025347:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 42732410 42735276 42732410 ID=MS.gene025347
chr2.2 mRNA 42732410 42735276 42732410 ID=MS.gene025347.t1;Parent=MS.gene025347
chr2.2 exon 42732410 42732589 42732410 ID=MS.gene025347.t1.exon1;Parent=MS.gene025347.t1
chr2.2 CDS 42732410 42732589 42732410 ID=cds.MS.gene025347.t1;Parent=MS.gene025347.t1
chr2.2 exon 42732841 42732963 42732841 ID=MS.gene025347.t1.exon2;Parent=MS.gene025347.t1
chr2.2 CDS 42732841 42732963 42732841 ID=cds.MS.gene025347.t1;Parent=MS.gene025347.t1
chr2.2 exon 42733332 42733414 42733332 ID=MS.gene025347.t1.exon3;Parent=MS.gene025347.t1
chr2.2 CDS 42733332 42733414 42733332 ID=cds.MS.gene025347.t1;Parent=MS.gene025347.t1
chr2.2 exon 42733547 42733656 42733547 ID=MS.gene025347.t1.exon4;Parent=MS.gene025347.t1
chr2.2 CDS 42733547 42733656 42733547 ID=cds.MS.gene025347.t1;Parent=MS.gene025347.t1
chr2.2 exon 42735008 42735098 42735008 ID=MS.gene025347.t1.exon5;Parent=MS.gene025347.t1
chr2.2 CDS 42735008 42735098 42735008 ID=cds.MS.gene025347.t1;Parent=MS.gene025347.t1
chr2.2 exon 42735180 42735276 42735180 ID=MS.gene025347.t1.exon6;Parent=MS.gene025347.t1
chr2.2 CDS 42735180 42735276 42735180 ID=cds.MS.gene025347.t1;Parent=MS.gene025347.t1
Gene Sequence

>MS.gene025347

ATGGGTTCAAGATCTACGGAAGGAGAACAATCATCGCTCGCTGACGATTATGATGCCTTGATTTCAACCACTGATGTTGAGCTTCTCAAGCGAGCATGGCGTAACGAAAAGGCAGCCCCCGAGATTCTTCGTTTTGAATCTGATTTGATCAACCGCGTCAAAGGACAGATTGAATTGATGGTAACGTTTCGAAAACTTGTTACCTTCTACTTTTTGTGATTTCTTATGATAGCTTACCAACTGAAATGAATTTCAATTTTGAAGACATGGATATTAAGTTCAAGTTTTTTTTTTATAAAAAATATTGAACTGAGAAAAGAAACTTAAATTTCTTATTGTCGGTTTTTCATTCATTAGTAACCTTTCATTGATGTTTTGGTGGTTTTCCTAGTTACTTCACATTTAATAAATGTGTTTTTCTCCTTTTACAGGAAGAAACTGTGGAAGAGAAATTGTCTGTTGGTAGTGACCCTCTTTCTGTATCTCTGTATCAGATGGACTTAGATAGAACCTTGTTTCTATTAAGGTCATATCTCCGCATCCGCATCCTGAAGGTAATCAGTCATAGGCTTTTAGCTTTTACACTCAAAGTGATAGGATTATTTATGGGTGTTTACTGTTAAGTCTAAATTAGTTCATTTGATAGAAATTAAGAAAAACAAACCAATAATTTATTGTTGCCTTACCCTCAAGTATTAACACCGGCCTTATTAATCTTTAACTAGATTGAAATTTGATTTTCGGTATAGCCTCTATTTGCGAGATAATATTTTTTACCACCTTAGTTTCTACCTATATCATAGGTTTTATGTAATTAAGATACACATTAATTGTTGAAATATTTGAATACTGGTAGCATGACCTATTGCTATATTCACTAGATTGCTTTTTGTTTAATTTTACAACCATATTTATATTGCAGATCGAAAAGTACATGTTCCACATCCGAAAAACTCAAGAACTTTGGAACAGACTTTCTAGGGATGAGAAACTTTTCACTGAAAGGTAAGGCTATCCAATGTGTTGTAAAAGATAGCTCAATGTGTTTTAACAAGAAATTTTTCTGACTATCAATGTGTTTTAAAAGATAGCAATGTAGGCTACTTGGTGTTTTTTAATCTAGTTTTCTGGGTGCAGGTGCACAGATGACCTGAAGGAACATCTAGAGAGCAGTGTTCTATCAAAACTACCTGAAAATTATCAGTCTTATGAGAGACAATCTGTCATTAGTGAAGAGGATGACATGGGTAATTTACCATTATTAGTTGTCCTTTTTGGTTTTGTTATTTCTTATGGACCATTTTGTGTCTGTCTAGGACTGTAGGTATTCAAATTTGATTTTTCTGAAATCAGTCTGTTATGAGAGAAAATTTCTTGTTACCATTTTGGCAAGTAATATGAAAGATTTTGAAATGATTGGATTGAATGAATTAAACTCAAAGTATGATGATCAATTCTGTAGCACTATTTTTTCAACATTTTATAAACTTCATGCTTTGTAGCTTTCTTTGCATATTTGATTTGAAGTTGTTTTAAAGTGCATGAATCCCTTAGGCTGTTCATCCTTCCATTACCAAATTAGGATGATGCTAAATGATTTTGATGTAATACTTTATGGTGATGGCTATAGAGATTATTTTTATATCCCTGTAAGCACTTAATGACCTTTGGTTGTGCTTAATTTTGAATATTTTGATAGCTAATCCTCAAAGGTCACTTTTACATTCAGAATCTTTCATTAACCATGGAAACCTAGAAATTTGAAAAGTCTCATTAACCATGCAAACCTAAAAAATTGAAAAGAGAAGGGGCTAAATGAGAGTGTGGGCCAAAATGTGCAGTTAGCACATGAGCTGCCTAAAACCTACAGTGTGGAGAGTTTATGTTAAGAAACATAAGAAGGGTGAAGGAAAAAATGTTGATGTGGCAGAGAGAGATGGATCATTTCTGTGATGGGTTCTGTTATATTTGGATATGAAGAAGATAGTGGGATATCTTGGAGAATCATTTAGTTAGTTATGGGAAGAGAGCATCTGTTCTCGGAATAAGCTTAGCACTGGGCAACACCTAACGAACTTTATTACTGCTTGCAACTGGCTATTTTATAAACCTTGTAGCTTTACTGATAATAACTACAGTTGGACACAAGTCATGTAAAGAGAAGACCCGCGGATTCTGTAGTAAGGAGAGTAGATCAGATGGAGGGGAGTCAAATCACTAGAGGTAGAGGAAGACCTAGAAAAACTAGAAGAGAAACTATTGAGAAAGATCTAGAGATTAATGATTTGGATAGAAATATGGTATTTGATAGAACAGTCTGGCGTCGTTTAATCCATGTAGCCAACTCCACTTAGTGGGATAAGGCTTAGTTGTTGCGCATGCATTTAAATTATTGTAGATTCCTTTCCATGAAAAACCATATTAGAGAGGCTTCTCCCTCGTTTTTTTCCATTTGTGTTTTCTCTCTTGCTAATTGAGGGTTTGGATCTGCTTTACCATAAATCATGCTGTCCTTTTTATATGTTATGTCTTTGACACTAGGATGTCTCCTGACATAAACGAACCATCTACAATGACATTTTGTTTTGAATGTTTCAGTTCCAGCACCCCGACTAGACACGTTTGTTTTGTGTAGAAGTAAAGAATATCTCACAGGAATTCAACTTGAAGATGGACCTGTTGATGACAGGCAATTATCTTTTCCTCGCATGAATTTTTTTCTCCTGTTTTTTAAAAGTTAGAAAGCTGTTTAATTTGATTATGCTTGCAGGTCGAAGCTGTTTGAGATGGAGCCTGATGTCCTTTACTTCATTTGTTACAAATCGATAAAACCACTTGTAGAGAGTGGGAAAATTGATCTACTATGA
Protein sequence

>MS.gene025347.t1

MGSRSTEGEQSSLADDYDALISTTDVELLKRAWRNEKAAPEILRFESDLINRVKGQIELMEETVEEKLSVGSDPLSVSLYQMDLDRTLFLLRSYLRIRILKIEKYMFHIRKTQELWNRLSRDEKLFTERCTDDLKEHLESSVLSKLPENYQSYERQSVISEEDDMVPAPRLDTFVLCRSKEYLTGIQLEDGPVDDRSKLFEMEPDVLYFICYKSIKPLVESGKIDLL