AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062116

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062116.t1 MTR_2g044210 99.119 227 2 0 1 227 1 227 2.35e-164 452
MS.gene062116.t1 MTR_2g044210 91.667 216 15 1 1 216 1 213 2.88e-141 395
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062116.t1 AT5G49010 58.590 227 87 2 1 227 1 220 5.92e-87 256
MS.gene062116.t1 AT5G49010 60.345 174 65 1 1 174 1 170 1.25e-66 204

Find 44 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGAACCTTATTTCTATTA+AGG 0.244460 2.3:+45743806 MS.gene062116:CDS
AACTTTGGAACAGACTTTCT+AGG 0.280314 2.3:+45744264 MS.gene062116:CDS
ATATGACCTTAATAGAAATA+AGG 0.379930 2.3:-45743812 None:intergenic
TGTTGGTAGTGACCCTCTTT+CGG 0.388310 2.3:+45743759 MS.gene062116:CDS
GAAAGGACAGATTGAATTGA+TGG 0.407740 2.3:+45743450 MS.gene062116:CDS
CAAAACAAACGTGTCTAGTC+GGG 0.413755 2.3:-45745913 None:intergenic
TGCTCTCTAGATGTTCCTTC+AGG 0.422302 2.3:-45744454 None:intergenic
AACATCAGTGGTTGAAATCA+AGG 0.435422 2.3:-45743347 None:intergenic
CAAATGAAGTAAAGGACATC+AGG 0.440202 2.3:-45746098 None:intergenic
GATAATTGCCTGTCATCAAC+AGG 0.456044 2.3:-45745981 None:intergenic
GATTCAAAACGAAGAATCTC+GGG 0.462194 2.3:-45743409 None:intergenic
ATCCATGGGTTCAAGATCTA+CGG 0.481878 2.3:+45743288 None:intergenic
ACTTTGGAACAGACTTTCTA+GGG 0.492617 2.3:+45744265 MS.gene062116:CDS
AACGTGTCTAGTCGGGGTGC+TGG 0.493562 2.3:-45745906 None:intergenic
AGATTCAAAACGAAGAATCT+CGG 0.501966 2.3:-45743410 None:intergenic
TTCGGTATCTCTGTATCAGA+TGG 0.507949 2.3:+45743777 MS.gene062116:CDS
GCGAGCATGGCGTAACGAAA+AGG 0.511669 2.3:+45743381 MS.gene062116:CDS
ATAAAACCACTTGTAGAGAG+TGG 0.514618 2.3:+45746131 MS.gene062116:CDS
GATTTGATCAACCGCGTGAA+AGG 0.518624 2.3:+45743433 MS.gene062116:CDS
TTCCGTAGATCTTGAACCCA+TGG 0.527155 2.3:-45743290 None:intergenic
CAGGTCGAAGCTGTTTGAGA+TGG 0.531476 2.3:+45746073 MS.gene062116:intron
ATTTGTAACAAATGAAGTAA+AGG 0.537264 2.3:-45746106 None:intergenic
GACTGATTACCTTCAGGATG+CGG 0.542811 2.3:-45743843 None:intergenic
ACAGGAATTCAACTTGAAGA+TGG 0.546681 2.3:+45745957 MS.gene062116:CDS
ACAAAACAAACGTGTCTAGT+CGG 0.547750 2.3:-45745914 None:intergenic
CATTAGTGAAGAGGATGACA+TGG 0.553868 2.3:+45744529 MS.gene062116:CDS
TTGAGCTTCTCAAGCGAGCA+TGG 0.560140 2.3:+45743368 MS.gene062116:CDS
ACAATCTGTCATTAGTGAAG+AGG 0.570258 2.3:+45744520 MS.gene062116:CDS
AAGATGGACCTGTTGATGAC+AGG 0.572063 2.3:+45745973 MS.gene062116:CDS
TCTTCGCATCCGCATCCTGA+AGG 0.576234 2.3:+45743834 MS.gene062116:CDS
TGATACAGAGATACCGAAAG+AGG 0.579183 2.3:-45743772 None:intergenic
TAAAACCACTTGTAGAGAGT+GGG 0.580787 2.3:+45746132 MS.gene062116:CDS
AGAAGTAAAGAATATCTCAC+AGG 0.604102 2.3:+45745939 MS.gene062116:CDS
CTTGAGAAGCTCAACATCAG+TGG 0.611012 2.3:-45743359 None:intergenic
GATACAGAGATACCGAAAGA+GGG 0.651435 2.3:-45743771 None:intergenic
CAGGTGCACAGATGACCTGA+AGG 0.652118 2.3:+45744439 MS.gene062116:intron
AAAACAAACGTGTCTAGTCG+GGG 0.662409 2.3:-45745912 None:intergenic
ATGGGTTCAAGATCTACGGA+AGG 0.674495 2.3:+45743292 MS.gene062116:CDS
GTGGAGGAGAAATTGTCTGT+TGG 0.675278 2.3:+45743742 MS.gene062116:CDS
ATTCAAAACGAAGAATCTCG+GGG 0.677114 2.3:-45743408 None:intergenic
TTTACAGGAAGAAACTGTGG+AGG 0.680524 2.3:+45743726 MS.gene062116:intron
ATTAGTGAAGAGGATGACAT+GGG 0.687742 2.3:+45744530 MS.gene062116:CDS
ATTCAATCTGTCCTTTCACG+CGG 0.698247 2.3:-45743444 None:intergenic
TTCAAAACGAAGAATCTCGG+GGG 0.737687 2.3:-45743407 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCTAGTTAAAGATTAATA+AGG - chr2.3:45744010-45744029 None:intergenic 15.0%
!! AAAGCAATAGGATTATTTAT+GGG + chr2.3:45743889-45743908 MS.gene062116:intron 20.0%
!! AAATTGAAATTCATTTCAGT+TGG - chr2.3:45743531-45743550 None:intergenic 20.0%
!! AAGAAATAACAAAACCAAAA+AGG - chr2.3:45744577-45744596 None:intergenic 20.0%
!! AGAATCATTTAGTTAGTTAT+GGG + chr2.3:45745305-45745324 MS.gene062116:intron 20.0%
!! ATATGACCTTAATAGAAATA+AGG - chr2.3:45743815-45743834 None:intergenic 20.0%
!! ATTTGTAACAAATGAAGTAA+AGG - chr2.3:45746109-45746128 None:intergenic 20.0%
!! CAAAGACATAACATATAAAA+AGG - chr2.3:45745820-45745839 None:intergenic 20.0%
!! CTAAAAAATTGAAAAGAGAA+GGG + chr2.3:45745092-45745111 MS.gene062116:intron 20.0%
!! TAAAAAATTGAAAAGAGAAG+GGG + chr2.3:45745093-45745112 MS.gene062116:intron 20.0%
!! TATGTTAAGAAACATAAGAA+GGG + chr2.3:45745186-45745205 MS.gene062116:intron 20.0%
!! TTATGTTAAGAAACATAAGA+AGG + chr2.3:45745185-45745204 MS.gene062116:intron 20.0%
!! TTATTTAATGTGAAGTAACT+AGG - chr2.3:45743691-45743710 None:intergenic 20.0%
!!! AAAATTTCTTGTTACCATTT+TGG + chr2.3:45744671-45744690 MS.gene062116:intron 20.0%
!!! ATAGAACCTTATTTCTATTA+AGG + chr2.3:45743806-45743825 MS.gene062116:CDS 20.0%
!!! ATCATAGGTTTTATGTAATT+AGG + chr2.3:45744099-45744118 MS.gene062116:intron 20.0%
!!! ATGAAAGATTTTGAAATGAT+TGG + chr2.3:45744702-45744721 MS.gene062116:intron 20.0%
!!! CTTTCTAACTTTTAAAAAAC+AGG - chr2.3:45746031-45746050 None:intergenic 20.0%
!!! GATTTTGATGTAATACTTTA+TGG + chr2.3:45744900-45744919 MS.gene062116:intron 20.0%
!!! GTTTTTTAATCTAGTTTTCT+GGG + chr2.3:45744414-45744433 MS.gene062116:intron 20.0%
!!! TAATGATTTGGATAGAAATA+TGG + chr2.3:45745583-45745602 MS.gene062116:intron 20.0%
!!! TGTTTTTTAATCTAGTTTTC+TGG + chr2.3:45744413-45744432 MS.gene062116:intron 20.0%
!!! TTTGGTTTTGTTATTTCTTA+TGG + chr2.3:45744578-45744597 MS.gene062116:intron 20.0%
! AAACAAAATGTCATTGTAGA+TGG - chr2.3:45745872-45745891 None:intergenic 25.0%
! AAGAGATCACAAAAAGTAAA+AGG - chr2.3:45743497-45743516 None:intergenic 25.0%
! AGATCTAGAGATTAATGATT+TGG + chr2.3:45745571-45745590 MS.gene062116:intron 25.0%
! CAAAGCAATAGGATTATTTA+TGG + chr2.3:45743888-45743907 MS.gene062116:intron 25.0%
! CCTAAAAAATTGAAAAGAGA+AGG + chr2.3:45745091-45745110 MS.gene062116:intron 25.0%
! GAGAATCATTTAGTTAGTTA+TGG + chr2.3:45745304-45745323 MS.gene062116:intron 25.0%
! GTAAGGCAACAATAAATTAT+TGG - chr2.3:45743968-45743987 None:intergenic 25.0%
! TTACATAAAACCTATGATAC+AGG - chr2.3:45744097-45744116 None:intergenic 25.0%
! TTACTGATAATAACTACAGT+TGG + chr2.3:45745424-45745443 MS.gene062116:intron 25.0%
! TTATTATCAGTAAAGCTACA+AGG - chr2.3:45745417-45745436 None:intergenic 25.0%
! TTCGATCTGCAATATAAATA+TGG - chr2.3:45744213-45744232 None:intergenic 25.0%
! TTTCATATTACTTGCCAAAA+TGG - chr2.3:45744688-45744707 None:intergenic 25.0%
!! ATGAGACTTTTCAAATTTCT+AGG - chr2.3:45745059-45745078 None:intergenic 25.0%
!! CCTTCTCTTTTCAATTTTTT+AGG - chr2.3:45745094-45745113 None:intergenic 25.0%
!! TAATATGGTTTTTCATGGAA+AGG - chr2.3:45745709-45745728 None:intergenic 25.0%
!! TCATAAGACTGATAATTTTC+AGG - chr2.3:45744497-45744516 None:intergenic 25.0%
!!! CTAGATTGCAATTTGATTTT+CGG + chr2.3:45744023-45744042 MS.gene062116:intron 25.0%
!!! GAATTTCAATTTTGAAGACA+TGG + chr2.3:45743539-45743558 MS.gene062116:intron 25.0%
!!! GTTTTAAAAGATAGCAATGT+AGG + chr2.3:45744382-45744401 MS.gene062116:intron 25.0%
!!! TTCAATTTTTTAGGTTTGCA+TGG - chr2.3:45745085-45745104 None:intergenic 25.0%
!!! TTTAATTTGATTATGCTTGC+AGG + chr2.3:45746054-45746073 MS.gene062116:intron 25.0%
AAAAAGGACAGCATGATTTA+TGG - chr2.3:45745804-45745823 None:intergenic 30.0%
AAGCAGTAATAAAGTTCGTT+AGG - chr2.3:45745374-45745393 None:intergenic 30.0%
AATGTTATCTCGCAAATTAG+AGG - chr2.3:45744054-45744073 None:intergenic 30.0%
ACACAAATGGAAAAAAAACG+AGG - chr2.3:45745744-45745763 None:intergenic 30.0%
AGAAGTAAAGAATATCTCAC+AGG + chr2.3:45745939-45745958 MS.gene062116:CDS 30.0%
AGATTCAAAACGAAGAATCT+CGG - chr2.3:45743413-45743432 None:intergenic 30.0%
CACAAATGGAAAAAAAACGA+GGG - chr2.3:45745743-45745762 None:intergenic 30.0%
CATGAAAAACCATATTAGAG+AGG + chr2.3:45745712-45745731 MS.gene062116:intron 30.0%
CATTTAGCATCATCCTAATT+TGG - chr2.3:45744881-45744900 None:intergenic 30.0%
CCAAAAAGGACAACTAATAA+TGG - chr2.3:45744563-45744582 None:intergenic 30.0%
GATGTAATACTTTATGGTGA+TGG + chr2.3:45744906-45744925 MS.gene062116:intron 30.0%
TATGATACAGGTAGAAACTA+AGG - chr2.3:45744085-45744104 None:intergenic 30.0%
TATGTTATGTCTTTGACACT+AGG + chr2.3:45745825-45745844 MS.gene062116:intron 30.0%
TCAAAATTAAGCACAACCAA+AGG - chr2.3:45744972-45744991 None:intergenic 30.0%
TCAGAATCTTTCATTAACCA+TGG + chr2.3:45745030-45745049 MS.gene062116:intron 30.0%
TGAAGGAAAAAATGTTGATG+TGG + chr2.3:45745209-45745228 MS.gene062116:intron 30.0%
TTAGTTTCTACCTGTATCAT+AGG + chr2.3:45744084-45744103 MS.gene062116:intron 30.0%
TTGGATATGAAGAAGATAGT+GGG + chr2.3:45745273-45745292 MS.gene062116:intron 30.0%
TTTGGATATGAAGAAGATAG+TGG + chr2.3:45745272-45745291 MS.gene062116:intron 30.0%
! AAACTTTTCACTGAAAGGTA+AGG + chr2.3:45744293-45744312 MS.gene062116:intron 30.0%
! AGCTATCTTTTACAACACAT+TGG - chr2.3:45744323-45744342 None:intergenic 30.0%
! ATGAGAAACTTTTCACTGAA+AGG + chr2.3:45744288-45744307 MS.gene062116:CDS 30.0%
! ATGTGTTTTTCTCCTTTTAC+AGG + chr2.3:45743711-45743730 MS.gene062116:intron 30.0%
! CCATTATTAGTTGTCCTTTT+TGG + chr2.3:45744560-45744579 MS.gene062116:intron 30.0%
! CTCTCTAATATGGTTTTTCA+TGG - chr2.3:45745714-45745733 None:intergenic 30.0%
! GAATGTAAAAGTGACTTTTG+AGG - chr2.3:45745012-45745031 None:intergenic 30.0%
! TCCAAAGTTCTTGAGTTTTT+CGG - chr2.3:45744253-45744272 None:intergenic 30.0%
! TTCAAATTTCTAGGTTTCCA+TGG - chr2.3:45745050-45745069 None:intergenic 30.0%
! TTTTCTCTCTTGCTAATTGA+GGG + chr2.3:45745763-45745782 MS.gene062116:intron 30.0%
!!! GTTTCTCTTCTAGTTTTTCT+AGG - chr2.3:45745542-45745561 None:intergenic 30.0%
AAAGGTCATTAAGTGCTTAC+AGG - chr2.3:45744954-45744973 None:intergenic 35.0%
AACATCAGTGGTTGAAATCA+AGG - chr2.3:45743350-45743369 None:intergenic 35.0%
AAGAAACATAAGAAGGGTGA+AGG + chr2.3:45745192-45745211 MS.gene062116:intron 35.0%
AAGGTCATTAAGTGCTTACA+GGG - chr2.3:45744953-45744972 None:intergenic 35.0%
ACAAAACAAACGTGTCTAGT+CGG - chr2.3:45745917-45745936 None:intergenic 35.0%
ACAATCTGTCATTAGTGAAG+AGG + chr2.3:45744520-45744539 MS.gene062116:CDS 35.0%
ACAGGAATTCAACTTGAAGA+TGG + chr2.3:45745957-45745976 MS.gene062116:CDS 35.0%
ACTGTTAATACTTGAGGGTA+AGG - chr2.3:45743985-45744004 None:intergenic 35.0%
ACTTTATTACTGCTTGCAAC+TGG + chr2.3:45745379-45745398 MS.gene062116:intron 35.0%
AGATGGATCATTTCTGTGAT+GGG + chr2.3:45745239-45745258 MS.gene062116:intron 35.0%
AGCAAGAGAGAAAACACAAA+TGG - chr2.3:45745757-45745776 None:intergenic 35.0%
AGGAGAAAAAAATTCATGCG+AGG - chr2.3:45746011-45746030 None:intergenic 35.0%
ATAAAACCACTTGTAGAGAG+TGG + chr2.3:45746131-45746150 MS.gene062116:CDS 35.0%
ATTAGTGAAGAGGATGACAT+GGG + chr2.3:45744530-45744549 MS.gene062116:CDS 35.0%
ATTCAAAACGAAGAATCTCG+GGG - chr2.3:45743411-45743430 None:intergenic 35.0%
ATTGCTGTGTATTCACTAGA+TGG + chr2.3:45744167-45744186 MS.gene062116:intron 35.0%
CAAATGAAGTAAAGGACATC+AGG - chr2.3:45746101-45746120 None:intergenic 35.0%
CATCCTTCCATTACCAAATT+AGG + chr2.3:45744865-45744884 MS.gene062116:intron 35.0%
GAAAGGACAGATTGAATTGA+TGG + chr2.3:45743450-45743469 MS.gene062116:CDS 35.0%
GATTCAAAACGAAGAATCTC+GGG - chr2.3:45743412-45743431 None:intergenic 35.0%
GCATCATCCTAATTTGGTAA+TGG - chr2.3:45744875-45744894 None:intergenic 35.0%
GTAACCTTTCATTGATGCTT+TGG + chr2.3:45743658-45743677 MS.gene062116:intron 35.0%
TAAAACCACTTGTAGAGAGT+GGG + chr2.3:45746132-45746151 MS.gene062116:CDS 35.0%
TCCGAAAAACTCAAGAACTT+TGG + chr2.3:45744249-45744268 MS.gene062116:CDS 35.0%
TCTAGTGAATACACAGCAAT+AGG - chr2.3:45744167-45744186 None:intergenic 35.0%
TGGTATTTGATAGAACAGTC+TGG + chr2.3:45745603-45745622 MS.gene062116:intron 35.0%
TGTAAGCACTTAATGACCTT+TGG + chr2.3:45744953-45744972 MS.gene062116:intron 35.0%
TTAAAGTGCATGAATCCCTT+AGG + chr2.3:45744837-45744856 MS.gene062116:intron 35.0%
TTACCCTCAAGTATTAACAG+TGG + chr2.3:45743984-45744003 MS.gene062116:intron 35.0%
! GCTTTTACACTCAAAGCAAT+AGG + chr2.3:45743877-45743896 MS.gene062116:intron 35.0%
! GTTTTCTCTCTTGCTAATTG+AGG + chr2.3:45745762-45745781 MS.gene062116:intron 35.0%
!! AACTTTGGAACAGACTTTCT+AGG + chr2.3:45744264-45744283 MS.gene062116:CDS 35.0%
!! AAGAAGATAGTGGGATATCT+TGG + chr2.3:45745282-45745301 MS.gene062116:intron 35.0%
!! ACTTTGGAACAGACTTTCTA+GGG + chr2.3:45744265-45744284 MS.gene062116:CDS 35.0%
!! CATCCTAATTTGGTAATGGA+AGG - chr2.3:45744871-45744890 None:intergenic 35.0%
!! GTGATGGGTTCTGTTATATT+TGG + chr2.3:45745254-45745273 MS.gene062116:intron 35.0%
AAAACAAACGTGTCTAGTCG+GGG - chr2.3:45745915-45745934 None:intergenic 40.0%
AAGGCCACTGTTAATACTTG+AGG - chr2.3:45743991-45744010 None:intergenic 40.0%
ACATAAACTCTCCACACTGT+AGG - chr2.3:45745171-45745190 None:intergenic 40.0%
ACCTTTCATTGATGCTTTGG+TGG + chr2.3:45743661-45743680 MS.gene062116:intron 40.0%
AGATAGCAATGTAGGCTACT+TGG + chr2.3:45744390-45744409 MS.gene062116:intron 40.0%
AGGCCACTGTTAATACTTGA+GGG - chr2.3:45743990-45744009 None:intergenic 40.0%
AGTAAGGAGAGTAGATCAGA+TGG + chr2.3:45745484-45745503 MS.gene062116:intron 40.0%
AGTCAAATCACTAGAGGTAG+AGG + chr2.3:45745512-45745531 MS.gene062116:intron 40.0%
AGTCCTAGACAGACACAAAA+TGG - chr2.3:45744605-45744624 None:intergenic 40.0%
ATTCAATCTGTCCTTTCACG+CGG - chr2.3:45743447-45743466 None:intergenic 40.0%
CAAAACAAACGTGTCTAGTC+GGG - chr2.3:45745916-45745935 None:intergenic 40.0%
CATTAGTGAAGAGGATGACA+TGG + chr2.3:45744529-45744548 MS.gene062116:CDS 40.0%
CCACAGTTTCTTCCTGTAAA+AGG - chr2.3:45743726-45743745 None:intergenic 40.0%
GAGATGGATCATTTCTGTGA+TGG + chr2.3:45745238-45745257 MS.gene062116:intron 40.0%
GATAATTGCCTGTCATCAAC+AGG - chr2.3:45745984-45746003 None:intergenic 40.0%
GATACAGAGATACCGAAAGA+GGG - chr2.3:45743774-45743793 None:intergenic 40.0%
GATACAGGTAGAAACTAAGG+TGG - chr2.3:45744082-45744101 None:intergenic 40.0%
GTAGATGGTTCGTTTATGTC+AGG - chr2.3:45745857-45745876 None:intergenic 40.0%
TCCTGAAGGTAATCAGTCAT+AGG + chr2.3:45743848-45743867 MS.gene062116:intron 40.0%
TCTCTTGCTAATTGAGGGTT+TGG + chr2.3:45745768-45745787 MS.gene062116:intron 40.0%
TGATACAGAGATACCGAAAG+AGG - chr2.3:45743775-45743794 None:intergenic 40.0%
TTCAAAACGAAGAATCTCGG+GGG - chr2.3:45743410-45743429 None:intergenic 40.0%
TTCGGTATCTCTGTATCAGA+TGG + chr2.3:45743777-45743796 MS.gene062116:CDS 40.0%
TTTACAGGAAGAAACTGTGG+AGG + chr2.3:45743726-45743745 MS.gene062116:intron 40.0%
! ACCACCAAAGCATCAATGAA+AGG - chr2.3:45743665-45743684 None:intergenic 40.0%
! ATTTTCCCACTCTCTACAAG+TGG - chr2.3:45746140-45746159 None:intergenic 40.0%
! CATGTGCTAACTGCACATTT+TGG - chr2.3:45745136-45745155 None:intergenic 40.0%
! CCTTTTACAGGAAGAAACTG+TGG + chr2.3:45743723-45743742 MS.gene062116:intron 40.0%
!! GTTCTTGAGTTTTTCGGATG+TGG - chr2.3:45744247-45744266 None:intergenic 40.0%
!!! TAATCTAGTTTTCTGGGTGC+AGG + chr2.3:45744420-45744439 MS.gene062116:intron 40.0%
AAGGAGAGTAGATCAGATGG+AGG + chr2.3:45745487-45745506 MS.gene062116:intron 45.0%
ACTAAGTGGAGTTGGCTACA+TGG - chr2.3:45745640-45745659 None:intergenic 45.0%
AGGAGAGTAGATCAGATGGA+GGG + chr2.3:45745488-45745507 MS.gene062116:intron 45.0%
AGGGAGAAGCCTCTCTAATA+TGG - chr2.3:45745724-45745743 None:intergenic 45.0%
ATGGAAGGATGAACAGCCTA+AGG - chr2.3:45744856-45744875 None:intergenic 45.0%
ATGGGTTCAAGATCTACGGA+AGG + chr2.3:45743292-45743311 MS.gene062116:CDS 45.0%
ATGTAGCCAACTCCACTTAG+TGG + chr2.3:45745639-45745658 MS.gene062116:intron 45.0%
CAACTCCACTTAGTGGGATA+AGG + chr2.3:45745646-45745665 MS.gene062116:intron 45.0%
CTCGGAATAAGCTTAGCACT+GGG + chr2.3:45745343-45745362 MS.gene062116:intron 45.0%
CTTATCCCACTAAGTGGAGT+TGG - chr2.3:45745648-45745667 None:intergenic 45.0%
CTTGAGAAGCTCAACATCAG+TGG - chr2.3:45743362-45743381 None:intergenic 45.0%
GACTGATTACCTTCAGGATG+CGG - chr2.3:45743846-45743865 None:intergenic 45.0%
GATTTGATCAACCGCGTGAA+AGG + chr2.3:45743433-45743452 MS.gene062116:CDS 45.0%
GCCTATGACTGATTACCTTC+AGG - chr2.3:45743852-45743871 None:intergenic 45.0%
GTGGAGGAGAAATTGTCTGT+TGG + chr2.3:45743742-45743761 MS.gene062116:CDS 45.0%
TCATGTAAAGAGAAGACCCG+CGG + chr2.3:45745454-45745473 MS.gene062116:intron 45.0%
TCTCCTTACTACAGAATCCG+CGG - chr2.3:45745474-45745493 None:intergenic 45.0%
TCTCGGAATAAGCTTAGCAC+TGG + chr2.3:45745342-45745361 MS.gene062116:intron 45.0%
TGCTCTCTAGATGTTCCTTC+AGG - chr2.3:45744457-45744476 None:intergenic 45.0%
TGGAAGGATGAACAGCCTAA+GGG - chr2.3:45744855-45744874 None:intergenic 45.0%
TGTAGCCAACTCCACTTAGT+GGG + chr2.3:45745640-45745659 MS.gene062116:intron 45.0%
! AAGGGGCTAAATGAGAGTGT+GGG + chr2.3:45745110-45745129 MS.gene062116:intron 45.0%
! CTAAGCCTTATCCCACTAAG+TGG - chr2.3:45745654-45745673 None:intergenic 45.0%
! CTCTCCACACTGTAGGTTTT+AGG - chr2.3:45745164-45745183 None:intergenic 45.0%
! GGACCATTTTGTGTCTGTCT+AGG + chr2.3:45744599-45744618 MS.gene062116:intron 45.0%
! TTGTGTCTGTCTAGGACTGT+AGG + chr2.3:45744607-45744626 MS.gene062116:intron 45.0%
!! AAGATGGACCTGTTGATGAC+AGG + chr2.3:45745973-45745992 MS.gene062116:CDS 45.0%
!! TGTTGGTAGTGACCCTCTTT+CGG + chr2.3:45743759-45743778 MS.gene062116:CDS 45.0%
CAGGTCGAAGCTGTTTGAGA+TGG + chr2.3:45746073-45746092 MS.gene062116:intron 50.0%
CTCCTTACTACAGAATCCGC+GGG - chr2.3:45745473-45745492 None:intergenic 50.0%
GAAGGGGCTAAATGAGAGTG+TGG + chr2.3:45745109-45745128 MS.gene062116:intron 50.0%
GAGGGGAGTCAAATCACTAG+AGG + chr2.3:45745506-45745525 MS.gene062116:intron 50.0%
GCTGCCTAAAACCTACAGTG+TGG + chr2.3:45745157-45745176 MS.gene062116:intron 50.0%
GGAGAGTAGATCAGATGGAG+GGG + chr2.3:45745489-45745508 MS.gene062116:intron 50.0%
GTTGATGTGGCAGAGAGAGA+TGG + chr2.3:45745222-45745241 MS.gene062116:intron 50.0%
TTGAGCTTCTCAAGCGAGCA+TGG + chr2.3:45743368-45743387 MS.gene062116:CDS 50.0%
!! GGGAAGAGAGCATCTGTTCT+CGG + chr2.3:45745325-45745344 MS.gene062116:intron 50.0%
CAGGTGCACAGATGACCTGA+AGG + chr2.3:45744439-45744458 MS.gene062116:intron 55.0%
GACCCGCGGATTCTGTAGTA+AGG + chr2.3:45745468-45745487 MS.gene062116:intron 55.0%
GCGAGCATGGCGTAACGAAA+AGG + chr2.3:45743381-45743400 MS.gene062116:CDS 55.0%
TCTTCGCATCCGCATCCTGA+AGG + chr2.3:45743834-45743853 MS.gene062116:CDS 55.0%
AACGTGTCTAGTCGGGGTGC+TGG - chr2.3:45745909-45745928 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 45743292 45746172 45743292 ID=MS.gene062116
chr2.3 mRNA 45743292 45746172 45743292 ID=MS.gene062116.t1;Parent=MS.gene062116
chr2.3 exon 45743292 45743471 45743292 ID=MS.gene062116.t1.exon1;Parent=MS.gene062116.t1
chr2.3 CDS 45743292 45743471 45743292 ID=cds.MS.gene062116.t1;Parent=MS.gene062116.t1
chr2.3 exon 45743733 45743855 45743733 ID=MS.gene062116.t1.exon2;Parent=MS.gene062116.t1
chr2.3 CDS 45743733 45743855 45743733 ID=cds.MS.gene062116.t1;Parent=MS.gene062116.t1
chr2.3 exon 45744227 45744309 45744227 ID=MS.gene062116.t1.exon3;Parent=MS.gene062116.t1
chr2.3 CDS 45744227 45744309 45744227 ID=cds.MS.gene062116.t1;Parent=MS.gene062116.t1
chr2.3 exon 45744442 45744551 45744442 ID=MS.gene062116.t1.exon4;Parent=MS.gene062116.t1
chr2.3 CDS 45744442 45744551 45744442 ID=cds.MS.gene062116.t1;Parent=MS.gene062116.t1
chr2.3 exon 45745904 45745994 45745904 ID=MS.gene062116.t1.exon5;Parent=MS.gene062116.t1
chr2.3 CDS 45745904 45745994 45745904 ID=cds.MS.gene062116.t1;Parent=MS.gene062116.t1
chr2.3 exon 45746076 45746172 45746076 ID=MS.gene062116.t1.exon6;Parent=MS.gene062116.t1
chr2.3 CDS 45746076 45746172 45746076 ID=cds.MS.gene062116.t1;Parent=MS.gene062116.t1
Gene Sequence

>MS.gene062116

ATGGGTTCAAGATCTACGGAAGGAGAACAATCATCGCTCGCTGACGATTATGATGCCTTGATTTCAACCACTGATGTTGAGCTTCTCAAGCGAGCATGGCGTAACGAAAAGGCAGCCCCCGAGATTCTTCGTTTTGAATCTGATTTGATCAACCGCGTGAAAGGACAGATTGAATTGATGGTAACGTTTCAAAAACTCGTTACCTTTTACTTTTTGTGATCTCTTATGATAGCTTACCAACTGAAATGAATTTCAATTTTGAAGACATGGATATAAAGTTCAAGTTTTTATTTTTTATTTTTTTATCAAAAATATTGAACTGAGAAAAGAAACTTAAATTTCTTATTGTCGTTTTTTCATTCATTAGTAACCTTTCATTGATGCTTTGGTGGTTTTCCTAGTTACTTCACATTAAATAAATGTGTTTTTCTCCTTTTACAGGAAGAAACTGTGGAGGAGAAATTGTCTGTTGGTAGTGACCCTCTTTCGGTATCTCTGTATCAGATGGACTTAGATAGAACCTTATTTCTATTAAGGTCATATCTTCGCATCCGCATCCTGAAGGTAATCAGTCATAGGCTTTTAGCTTTTACACTCAAAGCAATAGGATTATTTATGGGTGTTTACTGTTAAGTCTAAATTAGTTCATTTGATAGAAATTAAGAAAAACGAACCAATAATTTATTGTTGCCTTACCCTCAAGTATTAACAGTGGCCTTATTAATCTTTAACTAGATTGCAATTTGATTTTCGGTATAGCCTCTAATTTGCGAGATAACATTTTTTACCACCTTAGTTTCTACCTGTATCATAGGTTTTATGTAATTAGGATACACATTAATTGTTGAAATATTTGAATACTGATAGCATGACCTATTGCTGTGTATTCACTAGATGGCATTTTGTTTAATTTTATAACCATATTTATATTGCAGATCGAAAAGTACATGTTCCACATCCGAAAAACTCAAGAACTTTGGAACAGACTTTCTAGGGATGAGAAACTTTTCACTGAAAGGTAAGGCTATCCAATGTGTTGTAAAAGATAGCTCAATGTGTTTTAACAAGAAATTTTTCTGACTATCAATGTGTTTTAAAAGATAGCAATGTAGGCTACTTGGTGTTTTTTAATCTAGTTTTCTGGGTGCAGGTGCACAGATGACCTGAAGGAACATCTAGAGAGCAGTGTTCTATCAAAACTACCTGAAAATTATCAGTCTTATGAGAGACAATCTGTCATTAGTGAAGAGGATGACATGGGTAATTTACCATTATTAGTTGTCCTTTTTGGTTTTGTTATTTCTTATGGACCATTTTGTGTCTGTCTAGGACTGTAGGTATTCAAATTTGATTTTTCTGAAATCAGTCTGTTATGAGAGAAAATTTCTTGTTACCATTTTGGCAAGTAATATGAAAGATTTTGAAATGATTGGATTGAATGAATTAAACTCAAAGTATGATGATCAATTCTGTAGCACTATTTTTTCAACATTTTATAAACTTCATGCTTTGTAGCTTTCTTTGCATATTTGATTTGAAGTTGTTTTAAAGTGCATGAATCCCTTAGGCTGTTCATCCTTCCATTACCAAATTAGGATGATGCTAAATGATTTTGATGTAATACTTTATGGTGATGGCTATAGAGATTATTTTTATATCCCTGTAAGCACTTAATGACCTTTGGTTGTGCTTAATTTTGAATATTTTGATAGCTAATCCTCAAAAGTCACTTTTACATTCAGAATCTTTCATTAACCATGGAAACCTAGAAATTTGAAAAGTCTCATTAACCATGCAAACCTAAAAAATTGAAAAGAGAAGGGGCTAAATGAGAGTGTGGGCCAAAATGTGCAGTTAGCACATGAGCTGCCTAAAACCTACAGTGTGGAGAGTTTATGTTAAGAAACATAAGAAGGGTGAAGGAAAAAATGTTGATGTGGCAGAGAGAGATGGATCATTTCTGTGATGGGTTCTGTTATATTTGGATATGAAGAAGATAGTGGGATATCTTGGAGAATCATTTAGTTAGTTATGGGAAGAGAGCATCTGTTCTCGGAATAAGCTTAGCACTGGGCAACACCTAACGAACTTTATTACTGCTTGCAACTGGCTATTTTATAAACCTTGTAGCTTTACTGATAATAACTACAGTTGGACACAAGTCATGTAAAGAGAAGACCCGCGGATTCTGTAGTAAGGAGAGTAGATCAGATGGAGGGGAGTCAAATCACTAGAGGTAGAGGAAGACCTAGAAAAACTAGAAGAGAAACTATTGAGAAAGATCTAGAGATTAATGATTTGGATAGAAATATGGTATTTGATAGAACAGTCTGGCGTCGTTTAATCCATGTAGCCAACTCCACTTAGTGGGATAAGGCTTAGTTGTTGCGCGTGCATTTAAATTATTGTAGATTCCTTTCCATGAAAAACCATATTAGAGAGGCTTCTCCCTCGTTTTTTTTCCATTTGTGTTTTCTCTCTTGCTAATTGAGGGTTTGGATCTGCTTTACCATAAATCATGCTGTCCTTTTTATATGTTATGTCTTTGACACTAGGATGTCTCCTGACATAAACGAACCATCTACAATGACATTTTGTTTTGAATGTTTCAGTTCCAGCACCCCGACTAGACACGTTTGTTTTGTGTAGAAGTAAAGAATATCTCACAGGAATTCAACTTGAAGATGGACCTGTTGATGACAGGCAATTATCTTTTCCTCGCATGAATTTTTTTCTCCTGTTTTTTAAAAGTTAGAAAGCTGTTTAATTTGATTATGCTTGCAGGTCGAAGCTGTTTGAGATGGAGCCTGATGTCCTTTACTTCATTTGTTACAAATCGATAAAACCACTTGTAGAGAGTGGGAAAATTGATCTACTATGA
Protein sequence

>MS.gene062116.t1

MGSRSTEGEQSSLADDYDALISTTDVELLKRAWRNEKAAPEILRFESDLINRVKGQIELMEETVEEKLSVGSDPLSVSLYQMDLDRTLFLLRSYLRIRILKIEKYMFHIRKTQELWNRLSRDEKLFTERCTDDLKEHLESSVLSKLPENYQSYERQSVISEEDDMVPAPRLDTFVLCRSKEYLTGIQLEDGPVDDRSKLFEMEPDVLYFICYKSIKPLVESGKIDLL