AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85342

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85342.t1 MTR_2g044210 96.567 233 2 1 1 233 1 227 7.24e-162 446
MS.gene85342.t1 MTR_2g044210 89.189 222 15 2 1 222 1 213 1.16e-138 389
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85342.t1 AT5G49010 57.082 233 87 3 1 233 1 220 3.05e-84 250
MS.gene85342.t1 AT5G49010 58.333 180 65 2 1 180 1 170 5.76e-64 197

Find 43 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGAACCTTGTTTCTATTA+AGG 0.244460 2.1:+46847140 MS.gene85342:CDS
AACTTTGGAACAGACTTTCT+AGG 0.280314 2.1:+46847595 MS.gene85342:CDS
CAAAGGACAGATTGAATTGA+TGG 0.410021 2.1:+46846794 MS.gene85342:CDS
CAAAACAAACGTGTCTAGTC+GGG 0.413755 2.1:-46849243 None:intergenic
TGCTCTCTAGATGTTCCTTC+AGG 0.422302 2.1:-46847785 None:intergenic
AACATCAGTGGTTGAAATCA+AGG 0.435422 2.1:-46846691 None:intergenic
CAAATGAAGTAAAGGACATC+AGG 0.440202 2.1:-46849428 None:intergenic
GATAATTGCCTGTCATCAAC+AGG 0.456044 2.1:-46849311 None:intergenic
GATTCAAAACGAAGAATCTC+GGG 0.462194 2.1:-46846753 None:intergenic
TTCTGTATCTCTGTATCAGA+TGG 0.469476 2.1:+46847111 MS.gene85342:CDS
ATCCATGGGTTCAAGATCTA+CGG 0.481878 2.1:+46846632 None:intergenic
AACGTGTCTAGTCGGGGTGC+TGG 0.493562 2.1:-46849236 None:intergenic
AGATTCAAAACGAAGAATCT+CGG 0.501966 2.1:-46846754 None:intergenic
ACTTTGGAACAGACTTTCTA+GGG 0.506098 2.1:+46847596 MS.gene85342:CDS
GCGAGCATGGCGTAACGAAA+AGG 0.511669 2.1:+46846725 MS.gene85342:CDS
ATAAAACCACTTGTAGAGAG+TGG 0.514618 2.1:+46849461 MS.gene85342:CDS
GATTTGATCAACCGCGTCAA+AGG 0.524234 2.1:+46846777 MS.gene85342:CDS
TTCCGTAGATCTTGAACCCA+TGG 0.527155 2.1:-46846634 None:intergenic
TTACCTTCAGGATGCGGATG+CGG 0.527448 2.1:-46847171 None:intergenic
CAGGTCGAAGCTGTTTGAGA+TGG 0.531476 2.1:+46849403 MS.gene85342:intron
ATTTGTAACAAATGAAGTAA+AGG 0.537264 2.1:-46849436 None:intergenic
GACTGATTACCTTCAGGATG+CGG 0.542811 2.1:-46847177 None:intergenic
ACAGGAATTCAACTTGAAGA+TGG 0.546681 2.1:+46849287 MS.gene85342:CDS
ACAAAACAAACGTGTCTAGT+CGG 0.547750 2.1:-46849244 None:intergenic
CATTAGTGAAGAGGATGACA+TGG 0.553868 2.1:+46847860 MS.gene85342:CDS
TTGAGCTTCTCAAGCGAGCA+TGG 0.560140 2.1:+46846712 MS.gene85342:CDS
ACAATCTGTCATTAGTGAAG+AGG 0.570258 2.1:+46847851 MS.gene85342:CDS
AAGATGGACCTGTTGATGAC+AGG 0.572063 2.1:+46849303 MS.gene85342:CDS
TCTCCGCATCCGCATCCTGA+AGG 0.576234 2.1:+46847168 MS.gene85342:CDS
TAAAACCACTTGTAGAGAGT+GGG 0.580787 2.1:+46849462 MS.gene85342:CDS
TGATACAGAGATACAGAAAG+AGG 0.584753 2.1:-46847106 None:intergenic
AGAAGTAAAGAATATCTCAC+AGG 0.604102 2.1:+46849269 MS.gene85342:CDS
CTTGAGAAGCTCAACATCAG+TGG 0.611012 2.1:-46846703 None:intergenic
GATACAGAGATACAGAAAGA+GGG 0.651435 2.1:-46847105 None:intergenic
CAGGTGCACAGATGACCTGA+AGG 0.652118 2.1:+46847770 MS.gene85342:intron
GTGGAAGAGAAATTGTCTGT+TGG 0.657885 2.1:+46847076 MS.gene85342:CDS
AAAACAAACGTGTCTAGTCG+GGG 0.662409 2.1:-46849242 None:intergenic
ATGGGTTCAAGATCTACGGA+AGG 0.674495 2.1:+46846636 MS.gene85342:CDS
ATTCAAAACGAAGAATCTCG+GGG 0.677114 2.1:-46846752 None:intergenic
ATTCAATCTGTCCTTTGACG+CGG 0.682411 2.1:-46846788 None:intergenic
ATTAGTGAAGAGGATGACAT+GGG 0.687742 2.1:+46847861 MS.gene85342:CDS
ATATGACCTTAATAGAAACA+AGG 0.692807 2.1:-46847146 None:intergenic
TTCAAAACGAAGAATCTCGG+GGG 0.737687 2.1:-46846751 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTAAATTTCTTATTGT+CGG + chr2.1:46846955-46846974 MS.gene85342:intron 15.0%
!! AATCTAGTTAAAGATTAATA+AGG - chr2.1:46847344-46847363 None:intergenic 15.0%
!! AAAGTGATAGGATTATTTAT+GGG + chr2.1:46847223-46847242 MS.gene85342:intron 20.0%
!! AAATTGAAATTCATTTCAGT+TGG - chr2.1:46846875-46846894 None:intergenic 20.0%
!! AAGAAATAACAAAACCAAAA+AGG - chr2.1:46847908-46847927 None:intergenic 20.0%
!! AGAATCATTTAGTTAGTTAT+GGG + chr2.1:46848636-46848655 MS.gene85342:intron 20.0%
!! ATTTGTAACAAATGAAGTAA+AGG - chr2.1:46849439-46849458 None:intergenic 20.0%
!! CAAAGACATAACATATAAAA+AGG - chr2.1:46849150-46849169 None:intergenic 20.0%
!! CTAAAAAATTGAAAAGAGAA+GGG + chr2.1:46848423-46848442 MS.gene85342:intron 20.0%
!! TAAAAAATTGAAAAGAGAAG+GGG + chr2.1:46848424-46848443 MS.gene85342:intron 20.0%
!! TATGTTAAGAAACATAAGAA+GGG + chr2.1:46848517-46848536 MS.gene85342:intron 20.0%
!! TTACATAAAACCTATGATAT+AGG - chr2.1:46847430-46847449 None:intergenic 20.0%
!! TTATGTTAAGAAACATAAGA+AGG + chr2.1:46848516-46848535 MS.gene85342:intron 20.0%
!! TTATTAAATGTGAAGTAACT+AGG - chr2.1:46847025-46847044 None:intergenic 20.0%
!! TTGTTGAAATATTTGAATAC+TGG + chr2.1:46847466-46847485 MS.gene85342:intron 20.0%
!!! AAAATTTCTTGTTACCATTT+TGG + chr2.1:46848002-46848021 MS.gene85342:intron 20.0%
!!! ATGAAAGATTTTGAAATGAT+TGG + chr2.1:46848033-46848052 MS.gene85342:intron 20.0%
!!! CTAGATTGAAATTTGATTTT+CGG + chr2.1:46847357-46847376 MS.gene85342:intron 20.0%
!!! CTTTCTAACTTTTAAAAAAC+AGG - chr2.1:46849361-46849380 None:intergenic 20.0%
!!! GATTTTGATGTAATACTTTA+TGG + chr2.1:46848231-46848250 MS.gene85342:intron 20.0%
!!! GTTTTTTAATCTAGTTTTCT+GGG + chr2.1:46847745-46847764 MS.gene85342:intron 20.0%
!!! TAATGATTTGGATAGAAATA+TGG + chr2.1:46848914-46848933 MS.gene85342:intron 20.0%
!!! TGTTTTTTAATCTAGTTTTC+TGG + chr2.1:46847744-46847763 MS.gene85342:intron 20.0%
!!! TTTGGTTTTGTTATTTCTTA+TGG + chr2.1:46847909-46847928 MS.gene85342:intron 20.0%
! AAACAAAATGTCATTGTAGA+TGG - chr2.1:46849202-46849221 None:intergenic 25.0%
! AAATATTATCTCGCAAATAG+AGG - chr2.1:46847388-46847407 None:intergenic 25.0%
! AAGAAATCACAAAAAGTAGA+AGG - chr2.1:46846841-46846860 None:intergenic 25.0%
! AATCTAGTGAATATAGCAAT+AGG - chr2.1:46847500-46847519 None:intergenic 25.0%
! AGATCTAGAGATTAATGATT+TGG + chr2.1:46848902-46848921 MS.gene85342:intron 25.0%
! ATATGACCTTAATAGAAACA+AGG - chr2.1:46847149-46847168 None:intergenic 25.0%
! CAAAGTGATAGGATTATTTA+TGG + chr2.1:46847222-46847241 MS.gene85342:intron 25.0%
! CCTAAAAAATTGAAAAGAGA+AGG + chr2.1:46848422-46848441 MS.gene85342:intron 25.0%
! GAGAATCATTTAGTTAGTTA+TGG + chr2.1:46848635-46848654 MS.gene85342:intron 25.0%
! GTAAGGCAACAATAAATTAT+TGG - chr2.1:46847302-46847321 None:intergenic 25.0%
! TAGTTAAAGATTAATAAGGC+CGG - chr2.1:46847340-46847359 None:intergenic 25.0%
! TATGATATAGGTAGAAACTA+AGG - chr2.1:46847418-46847437 None:intergenic 25.0%
! TTACTGATAATAACTACAGT+TGG + chr2.1:46848755-46848774 MS.gene85342:intron 25.0%
! TTAGTTTCTACCTATATCAT+AGG + chr2.1:46847417-46847436 MS.gene85342:intron 25.0%
! TTATTATCAGTAAAGCTACA+AGG - chr2.1:46848748-46848767 None:intergenic 25.0%
! TTCGATCTGCAATATAAATA+TGG - chr2.1:46847544-46847563 None:intergenic 25.0%
! TTTCATATTACTTGCCAAAA+TGG - chr2.1:46848019-46848038 None:intergenic 25.0%
!! ATGAGACTTTTCAAATTTCT+AGG - chr2.1:46848390-46848409 None:intergenic 25.0%
!! CCTTCTCTTTTCAATTTTTT+AGG - chr2.1:46848425-46848444 None:intergenic 25.0%
!! TAATATGGTTTTTCATGGAA+AGG - chr2.1:46849040-46849059 None:intergenic 25.0%
!! TCATAAGACTGATAATTTTC+AGG - chr2.1:46847828-46847847 None:intergenic 25.0%
!!! ATAGAACCTTGTTTCTATTA+AGG + chr2.1:46847140-46847159 MS.gene85342:CDS 25.0%
!!! GAATTTCAATTTTGAAGACA+TGG + chr2.1:46846883-46846902 MS.gene85342:intron 25.0%
!!! GTTTTAAAAGATAGCAATGT+AGG + chr2.1:46847713-46847732 MS.gene85342:intron 25.0%
!!! TTCAATTTTTTAGGTTTGCA+TGG - chr2.1:46848416-46848435 None:intergenic 25.0%
!!! TTTAATTTGATTATGCTTGC+AGG + chr2.1:46849384-46849403 MS.gene85342:intron 25.0%
AAAAAGGACAGCATGATTTA+TGG - chr2.1:46849134-46849153 None:intergenic 30.0%
AACACAAATGGAAAAAAACG+AGG - chr2.1:46849075-46849094 None:intergenic 30.0%
AAGCAGTAATAAAGTTCGTT+AGG - chr2.1:46848705-46848724 None:intergenic 30.0%
ACACAAATGGAAAAAAACGA+GGG - chr2.1:46849074-46849093 None:intergenic 30.0%
AGAAGTAAAGAATATCTCAC+AGG + chr2.1:46849269-46849288 MS.gene85342:CDS 30.0%
AGATTCAAAACGAAGAATCT+CGG - chr2.1:46846757-46846776 None:intergenic 30.0%
CATGAAAAACCATATTAGAG+AGG + chr2.1:46849043-46849062 MS.gene85342:intron 30.0%
CATTTAGCATCATCCTAATT+TGG - chr2.1:46848212-46848231 None:intergenic 30.0%
CCAAAAAGGACAACTAATAA+TGG - chr2.1:46847894-46847913 None:intergenic 30.0%
GATGTAATACTTTATGGTGA+TGG + chr2.1:46848237-46848256 MS.gene85342:intron 30.0%
TATGTTATGTCTTTGACACT+AGG + chr2.1:46849155-46849174 MS.gene85342:intron 30.0%
TCAAAATTAAGCACAACCAA+AGG - chr2.1:46848303-46848322 None:intergenic 30.0%
TCAGAATCTTTCATTAACCA+TGG + chr2.1:46848361-46848380 MS.gene85342:intron 30.0%
TGAAGGAAAAAATGTTGATG+TGG + chr2.1:46848540-46848559 MS.gene85342:intron 30.0%
TTGGATATGAAGAAGATAGT+GGG + chr2.1:46848604-46848623 MS.gene85342:intron 30.0%
TTTGGATATGAAGAAGATAG+TGG + chr2.1:46848603-46848622 MS.gene85342:intron 30.0%
! AAACTTTTCACTGAAAGGTA+AGG + chr2.1:46847624-46847643 MS.gene85342:intron 30.0%
! AGCTATCTTTTACAACACAT+TGG - chr2.1:46847654-46847673 None:intergenic 30.0%
! ATGTGTTTTTCTCCTTTTAC+AGG + chr2.1:46847045-46847064 MS.gene85342:intron 30.0%
! CCATTATTAGTTGTCCTTTT+TGG + chr2.1:46847891-46847910 MS.gene85342:intron 30.0%
! CTCTCTAATATGGTTTTTCA+TGG - chr2.1:46849045-46849064 None:intergenic 30.0%
! GAATGTAAAAGTGACTTTTG+AGG - chr2.1:46848343-46848362 None:intergenic 30.0%
! GTAACCTTTCATTGATGTTT+TGG + chr2.1:46846992-46847011 MS.gene85342:intron 30.0%
! TCCAAAGTTCTTGAGTTTTT+CGG - chr2.1:46847584-46847603 None:intergenic 30.0%
! TTCAAATTTCTAGGTTTCCA+TGG - chr2.1:46848381-46848400 None:intergenic 30.0%
! TTTTCTCTCTTGCTAATTGA+GGG + chr2.1:46849093-46849112 MS.gene85342:intron 30.0%
!!! GTTTCTCTTCTAGTTTTTCT+AGG - chr2.1:46848873-46848892 None:intergenic 30.0%
AAAGGTCATTAAGTGCTTAC+AGG - chr2.1:46848285-46848304 None:intergenic 35.0%
AACATCAGTGGTTGAAATCA+AGG - chr2.1:46846694-46846713 None:intergenic 35.0%
AAGAAACATAAGAAGGGTGA+AGG + chr2.1:46848523-46848542 MS.gene85342:intron 35.0%
AAGGTCATTAAGTGCTTACA+GGG - chr2.1:46848284-46848303 None:intergenic 35.0%
ACAAAACAAACGTGTCTAGT+CGG - chr2.1:46849247-46849266 None:intergenic 35.0%
ACAATCTGTCATTAGTGAAG+AGG + chr2.1:46847851-46847870 MS.gene85342:CDS 35.0%
ACAGGAATTCAACTTGAAGA+TGG + chr2.1:46849287-46849306 MS.gene85342:CDS 35.0%
ACCACCAAAACATCAATGAA+AGG - chr2.1:46846999-46847018 None:intergenic 35.0%
ACTTTATTACTGCTTGCAAC+TGG + chr2.1:46848710-46848729 MS.gene85342:intron 35.0%
AGATGGATCATTTCTGTGAT+GGG + chr2.1:46848570-46848589 MS.gene85342:intron 35.0%
AGCAAGAGAGAAAACACAAA+TGG - chr2.1:46849087-46849106 None:intergenic 35.0%
AGGAGAAAAAAATTCATGCG+AGG - chr2.1:46849341-46849360 None:intergenic 35.0%
ATAAAACCACTTGTAGAGAG+TGG + chr2.1:46849461-46849480 MS.gene85342:CDS 35.0%
ATTAGTGAAGAGGATGACAT+GGG + chr2.1:46847861-46847880 MS.gene85342:CDS 35.0%
ATTCAAAACGAAGAATCTCG+GGG - chr2.1:46846755-46846774 None:intergenic 35.0%
CAAAGGACAGATTGAATTGA+TGG + chr2.1:46846794-46846813 MS.gene85342:CDS 35.0%
CAAATGAAGTAAAGGACATC+AGG - chr2.1:46849431-46849450 None:intergenic 35.0%
CATCCTTCCATTACCAAATT+AGG + chr2.1:46848196-46848215 MS.gene85342:intron 35.0%
GATACAGAGATACAGAAAGA+GGG - chr2.1:46847108-46847127 None:intergenic 35.0%
GATATAGGTAGAAACTAAGG+TGG - chr2.1:46847415-46847434 None:intergenic 35.0%
GATTCAAAACGAAGAATCTC+GGG - chr2.1:46846756-46846775 None:intergenic 35.0%
GCATCATCCTAATTTGGTAA+TGG - chr2.1:46848206-46848225 None:intergenic 35.0%
TAAAACCACTTGTAGAGAGT+GGG + chr2.1:46849462-46849481 MS.gene85342:CDS 35.0%
TCCGAAAAACTCAAGAACTT+TGG + chr2.1:46847580-46847599 MS.gene85342:CDS 35.0%
TGATACAGAGATACAGAAAG+AGG - chr2.1:46847109-46847128 None:intergenic 35.0%
TGGTATTTGATAGAACAGTC+TGG + chr2.1:46848934-46848953 MS.gene85342:intron 35.0%
TGTAAGCACTTAATGACCTT+TGG + chr2.1:46848284-46848303 MS.gene85342:intron 35.0%
TTAAAGTGCATGAATCCCTT+AGG + chr2.1:46848168-46848187 MS.gene85342:intron 35.0%
TTACCCTCAAGTATTAACAC+CGG + chr2.1:46847318-46847337 MS.gene85342:intron 35.0%
TTCTGTATCTCTGTATCAGA+TGG + chr2.1:46847111-46847130 MS.gene85342:CDS 35.0%
! ACGAGAAACTTTTCACTGAA+AGG + chr2.1:46847619-46847638 MS.gene85342:CDS 35.0%
! GCTTTTACACTCAAAGTGAT+AGG + chr2.1:46847211-46847230 MS.gene85342:intron 35.0%
! GTTTTCTCTCTTGCTAATTG+AGG + chr2.1:46849092-46849111 MS.gene85342:intron 35.0%
!! AACTTTGGAACAGACTTTCT+AGG + chr2.1:46847595-46847614 MS.gene85342:CDS 35.0%
!! AAGAAGATAGTGGGATATCT+TGG + chr2.1:46848613-46848632 MS.gene85342:intron 35.0%
!! ACCTTTCATTGATGTTTTGG+TGG + chr2.1:46846995-46847014 MS.gene85342:intron 35.0%
!! ACTTTGGAACAGACTTTCTA+GGG + chr2.1:46847596-46847615 MS.gene85342:CDS 35.0%
!! CATCCTAATTTGGTAATGGA+AGG - chr2.1:46848202-46848221 None:intergenic 35.0%
!! GTGATGGGTTCTGTTATATT+TGG + chr2.1:46848585-46848604 MS.gene85342:intron 35.0%
AAAACAAACGTGTCTAGTCG+GGG - chr2.1:46849245-46849264 None:intergenic 40.0%
AGATAGCAATGTAGGCTACT+TGG + chr2.1:46847721-46847740 MS.gene85342:intron 40.0%
AGTAAGGAGAGTAGATCAGA+TGG + chr2.1:46848815-46848834 MS.gene85342:intron 40.0%
AGTCAAATCACTAGAGGTAG+AGG + chr2.1:46848843-46848862 MS.gene85342:intron 40.0%
AGTCCTAGACAGACACAAAA+TGG - chr2.1:46847936-46847955 None:intergenic 40.0%
ATTCAATCTGTCCTTTGACG+CGG - chr2.1:46846791-46846810 None:intergenic 40.0%
CAAAACAAACGTGTCTAGTC+GGG - chr2.1:46849246-46849265 None:intergenic 40.0%
CATTAGTGAAGAGGATGACA+TGG + chr2.1:46847860-46847879 MS.gene85342:CDS 40.0%
CCACAGTTTCTTCCTGTAAA+AGG - chr2.1:46847060-46847079 None:intergenic 40.0%
CTTGGAATAAGCTTAGCACT+GGG + chr2.1:46848674-46848693 MS.gene85342:intron 40.0%
GAGATGGATCATTTCTGTGA+TGG + chr2.1:46848569-46848588 MS.gene85342:intron 40.0%
GATAATTGCCTGTCATCAAC+AGG - chr2.1:46849314-46849333 None:intergenic 40.0%
GGTGTTAATACTTGAGGGTA+AGG - chr2.1:46847319-46847338 None:intergenic 40.0%
GTAGATGGTTCGTTTATGTC+AGG - chr2.1:46849187-46849206 None:intergenic 40.0%
GTGGAAGAGAAATTGTCTGT+TGG + chr2.1:46847076-46847095 MS.gene85342:CDS 40.0%
TCCTGAAGGTAATCAGTCAT+AGG + chr2.1:46847182-46847201 MS.gene85342:intron 40.0%
TCTCTTGCTAATTGAGGGTT+TGG + chr2.1:46849098-46849117 MS.gene85342:intron 40.0%
TCTTGGAATAAGCTTAGCAC+TGG + chr2.1:46848673-46848692 MS.gene85342:intron 40.0%
TTCAAAACGAAGAATCTCGG+GGG - chr2.1:46846754-46846773 None:intergenic 40.0%
! ATTTTCCCACTCTCTACAAG+TGG - chr2.1:46849470-46849489 None:intergenic 40.0%
! CATGTGCTAACTGCACATTT+TGG - chr2.1:46848467-46848486 None:intergenic 40.0%
! CCTTTTACAGGAAGAAACTG+TGG + chr2.1:46847057-46847076 MS.gene85342:intron 40.0%
!! GTTCTTGAGTTTTTCGGATG+TGG - chr2.1:46847578-46847597 None:intergenic 40.0%
!!! TAATCTAGTTTTCTGGGTGC+AGG + chr2.1:46847751-46847770 MS.gene85342:intron 40.0%
AAGGAGAGTAGATCAGATGG+AGG + chr2.1:46848818-46848837 MS.gene85342:intron 45.0%
ACATAAACTCTCCACACCGT+AGG - chr2.1:46848502-46848521 None:intergenic 45.0%
ACTAAGTGGAGTTGGCTACA+TGG - chr2.1:46848971-46848990 None:intergenic 45.0%
AGGAGAGTAGATCAGATGGA+GGG + chr2.1:46848819-46848838 MS.gene85342:intron 45.0%
AGGGAGAAGCCTCTCTAATA+TGG - chr2.1:46849055-46849074 None:intergenic 45.0%
ATGGAAGGATGAACAGCCTA+AGG - chr2.1:46848187-46848206 None:intergenic 45.0%
ATGGGTTCAAGATCTACGGA+AGG + chr2.1:46846636-46846655 MS.gene85342:CDS 45.0%
ATGTAGCCAACTCCACTTAG+TGG + chr2.1:46848970-46848989 MS.gene85342:intron 45.0%
CAACTCCACTTAGTGGGATA+AGG + chr2.1:46848977-46848996 MS.gene85342:intron 45.0%
CATGAGCTGCCTAAAACCTA+CGG + chr2.1:46848483-46848502 MS.gene85342:intron 45.0%
CTTATCCCACTAAGTGGAGT+TGG - chr2.1:46848979-46848998 None:intergenic 45.0%
CTTGAGAAGCTCAACATCAG+TGG - chr2.1:46846706-46846725 None:intergenic 45.0%
GACTGATTACCTTCAGGATG+CGG - chr2.1:46847180-46847199 None:intergenic 45.0%
GATTTGATCAACCGCGTCAA+AGG + chr2.1:46846777-46846796 MS.gene85342:CDS 45.0%
GCCTATGACTGATTACCTTC+AGG - chr2.1:46847186-46847205 None:intergenic 45.0%
TCATGTAAAGAGAAGACCCG+CGG + chr2.1:46848785-46848804 MS.gene85342:intron 45.0%
TCTCCTTACTACAGAATCCG+CGG - chr2.1:46848805-46848824 None:intergenic 45.0%
TGCTCTCTAGATGTTCCTTC+AGG - chr2.1:46847788-46847807 None:intergenic 45.0%
TGGAAGGATGAACAGCCTAA+GGG - chr2.1:46848186-46848205 None:intergenic 45.0%
TGTAGCCAACTCCACTTAGT+GGG + chr2.1:46848971-46848990 MS.gene85342:intron 45.0%
! AAGGCCGGTGTTAATACTTG+AGG - chr2.1:46847325-46847344 None:intergenic 45.0%
! AAGGGGCTAAATGAGAGTGT+GGG + chr2.1:46848441-46848460 MS.gene85342:intron 45.0%
! AGGCCGGTGTTAATACTTGA+GGG - chr2.1:46847324-46847343 None:intergenic 45.0%
! CTAAGCCTTATCCCACTAAG+TGG - chr2.1:46848985-46849004 None:intergenic 45.0%
! GGACCATTTTGTGTCTGTCT+AGG + chr2.1:46847930-46847949 MS.gene85342:intron 45.0%
! TTGTGTCTGTCTAGGACTGT+AGG + chr2.1:46847938-46847957 MS.gene85342:intron 45.0%
!! AAGATGGACCTGTTGATGAC+AGG + chr2.1:46849303-46849322 MS.gene85342:CDS 45.0%
CAGGTCGAAGCTGTTTGAGA+TGG + chr2.1:46849403-46849422 MS.gene85342:intron 50.0%
CTCCTTACTACAGAATCCGC+GGG - chr2.1:46848804-46848823 None:intergenic 50.0%
GAAGGGGCTAAATGAGAGTG+TGG + chr2.1:46848440-46848459 MS.gene85342:intron 50.0%
GAGGGGAGTCAAATCACTAG+AGG + chr2.1:46848837-46848856 MS.gene85342:intron 50.0%
GGAGAGTAGATCAGATGGAG+GGG + chr2.1:46848820-46848839 MS.gene85342:intron 50.0%
GTTGATGTGGCAGAGAGAGA+TGG + chr2.1:46848553-46848572 MS.gene85342:intron 50.0%
TTACCTTCAGGATGCGGATG+CGG - chr2.1:46847174-46847193 None:intergenic 50.0%
TTGAGCTTCTCAAGCGAGCA+TGG + chr2.1:46846712-46846731 MS.gene85342:CDS 50.0%
! CTCTCCACACCGTAGGTTTT+AGG - chr2.1:46848495-46848514 None:intergenic 50.0%
!! GGGAAGAGAGCATCTGTTCT+TGG + chr2.1:46848656-46848675 MS.gene85342:intron 50.0%
CAGGTGCACAGATGACCTGA+AGG + chr2.1:46847770-46847789 MS.gene85342:intron 55.0%
GACCCGCGGATTCTGTAGTA+AGG + chr2.1:46848799-46848818 MS.gene85342:intron 55.0%
GCGAGCATGGCGTAACGAAA+AGG + chr2.1:46846725-46846744 MS.gene85342:CDS 55.0%
GCTGCCTAAAACCTACGGTG+TGG + chr2.1:46848488-46848507 MS.gene85342:intron 55.0%
AACGTGTCTAGTCGGGGTGC+TGG - chr2.1:46849239-46849258 None:intergenic 60.0%
TCTCCGCATCCGCATCCTGA+AGG + chr2.1:46847168-46847187 MS.gene85342:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 46846636 46849502 46846636 ID=MS.gene85342
chr2.1 mRNA 46846636 46849502 46846636 ID=MS.gene85342.t1;Parent=MS.gene85342
chr2.1 exon 46846636 46846833 46846636 ID=MS.gene85342.t1.exon1;Parent=MS.gene85342.t1
chr2.1 CDS 46846636 46846833 46846636 ID=cds.MS.gene85342.t1;Parent=MS.gene85342.t1
chr2.1 exon 46847067 46847189 46847067 ID=MS.gene85342.t1.exon2;Parent=MS.gene85342.t1
chr2.1 CDS 46847067 46847189 46847067 ID=cds.MS.gene85342.t1;Parent=MS.gene85342.t1
chr2.1 exon 46847558 46847640 46847558 ID=MS.gene85342.t1.exon3;Parent=MS.gene85342.t1
chr2.1 CDS 46847558 46847640 46847558 ID=cds.MS.gene85342.t1;Parent=MS.gene85342.t1
chr2.1 exon 46847773 46847882 46847773 ID=MS.gene85342.t1.exon4;Parent=MS.gene85342.t1
chr2.1 CDS 46847773 46847882 46847773 ID=cds.MS.gene85342.t1;Parent=MS.gene85342.t1
chr2.1 exon 46849234 46849324 46849234 ID=MS.gene85342.t1.exon5;Parent=MS.gene85342.t1
chr2.1 CDS 46849234 46849324 46849234 ID=cds.MS.gene85342.t1;Parent=MS.gene85342.t1
chr2.1 exon 46849406 46849502 46849406 ID=MS.gene85342.t1.exon6;Parent=MS.gene85342.t1
chr2.1 CDS 46849406 46849502 46849406 ID=cds.MS.gene85342.t1;Parent=MS.gene85342.t1
Gene Sequence

>MS.gene85342

ATGGGTTCAAGATCTACGGAAGGAGAACAATCATCGCTCGCTGACGATTATGATGCCTTGATTTCAACCACTGATGTTGAGCTTCTCAAGCGAGCATGGCGTAACGAAAAGGCAGCCCCCGAGATTCTTCGTTTTGAATCTGATTTGATCAACCGCGTCAAAGGACAGATTGAATTGATGGCAACGTTTCGAAAACTTGTTACCTTCTACTTTTTGTGATTTCTTATGATAGCTTACCAACTGAAATGAATTTCAATTTTGAAGACATGGATATTAAGTTCAAGTTTTTTTTTTATAAAAAATATTGAACTGAGAAAAGAAACTTAAATTTCTTATTGTCGGTTTTTCATTCATTAGTAACCTTTCATTGATGTTTTGGTGGTTTTCCTAGTTACTTCACATTTAATAAATGTGTTTTTCTCCTTTTACAGGAAGAAACTGTGGAAGAGAAATTGTCTGTTGGTAGCGACCCTCTTTCTGTATCTCTGTATCAGATGGACTTAGATAGAACCTTGTTTCTATTAAGGTCATATCTCCGCATCCGCATCCTGAAGGTAATCAGTCATAGGCTTTTAGCTTTTACACTCAAAGTGATAGGATTATTTATGGGTGTTTACTGTTAAGTCTAAATTAGTTCATTTGATAGAAATTAAGAAAAACAAACCAATAATTTATTGTTGCCTTACCCTCAAGTATTAACACCGGCCTTATTAATCTTTAACTAGATTGAAATTTGATTTTCGGTATAGCCTCTATTTGCGAGATAATATTTTTTACCACCTTAGTTTCTACCTATATCATAGGTTTTATGTAATTAAGATACACATTAATTGTTGAAATATTTGAATACTGGTAGCATGTCCTATTGCTATATTCACTAGATTGCTTTTTGTTTAATTTTACAACCATATTTATATTGCAGATCGAAAAGTACATGTTCCACATCCGAAAAACTCAAGAACTTTGGAACAGACTTTCTAGGGACGAGAAACTTTTCACTGAAAGGTAAGGCTATCCAATGTGTTGTAAAAGATAGCTCAATGTGTTTTAACAAGAAATTTTTCTGACTATCAATGTGTTTTAAAAGATAGCAATGTAGGCTACTTGGTGTTTTTTAATCTAGTTTTCTGGGTGCAGGTGCACAGATGACCTGAAGGAACATCTAGAGAGCAGTGTTCTATCAAAACTACCTGAAAATTATCAGTCTTATGAGAGACAATCTGTCATTAGTGAAGAGGATGACATGGGTAATTTACCATTATTAGTTGTCCTTTTTGGTTTTGTTATTTCTTATGGACCATTTTGTGTCTGTCTAGGACTGTAGGTATTCAAATCTGATTTTTCTGAAATCAGTCTGTTATGAGAGAAAATTTCTTGTTACCATTTTGGCAAGTAATATGAAAGATTTTGAAATGATTGGATTGAATGAATTAAACTCAAAGTATGATGATCAATTCTGTAGCACTATTTTTTCAACATTTTATAAACTTCATGCTTTGTAGCTTTCTTTGCATATTTGATTTGAAGTTGTTTTAAAGTGCATGAATCCCTTAGGCTGTTCATCCTTCCATTACCAAATTAGGATGATGCTAAATGATTTTGATGTAATACTTTATGGTGATGGCTATAGAGATTATTTTTATATCCCTGTAAGCACTTAATGACCTTTGGTTGTGCTTAATTTTGAATATTTTGATAGCTAATCCTCAAAAGTCACTTTTACATTCAGAATCTTTCATTAACCATGGAAACCTAGAAATTTGAAAAGTCTCATTAACCATGCAAACCTAAAAAATTGAAAAGAGAAGGGGCTAAATGAGAGTGTGGGCCAAAATGTGCAGTTAGCACATGAGCTGCCTAAAACCTACGGTGTGGAGAGTTTATGTTAAGAAACATAAGAAGGGTGAAGGAAAAAATGTTGATGTGGCAGAGAGAGATGGATCATTTCTGTGATGGGTTCTGTTATATTTGGATATGAAGAAGATAGTGGGATATCTTGGAGAATCATTTAGTTAGTTATGGGAAGAGAGCATCTGTTCTTGGAATAAGCTTAGCACTGGGCAACACCTAACGAACTTTATTACTGCTTGCAACTGGTTATTTTATAAACCTTGTAGCTTTACTGATAATAACTACAGTTGGACACAAGTCATGTAAAGAGAAGACCCGCGGATTCTGTAGTAAGGAGAGTAGATCAGATGGAGGGGAGTCAAATCACTAGAGGTAGAGGAAGACCTAGAAAAACTAGAAGAGAAACTATTGAGAAAGATCTAGAGATTAATGATTTGGATAGAAATATGGTATTTGATAGAACAGTCTGGCGTCGTTTAATCCATGTAGCCAACTCCACTTAGTGGGATAAGGCTTAGTTGTTGCGCGTGCATTTAAATTATTGTAGATTCCTTTCCATGAAAAACCATATTAGAGAGGCTTCTCCCTCGTTTTTTTCCATTTGTGTTTTCTCTCTTGCTAATTGAGGGTTTGGATCTGCTTTACCATAAATCATGCTGTCCTTTTTATATGTTATGTCTTTGACACTAGGATGTCTCCTGACATAAACGAACCATCTACAATGACATTTTGTTTTGAATGTTTCAGTTCCAGCACCCCGACTAGACACGTTTGTTTTGTGTAGAAGTAAAGAATATCTCACAGGAATTCAACTTGAAGATGGACCTGTTGATGACAGGCAATTATCTTTTCCTCGCATGAATTTTTTTCTCCTGTTTTTTAAAAGTTAGAAAGCTGTTTAATTTGATTATGCTTGCAGGTCGAAGCTGTTTGAGATGGAGCCTGATGTCCTTTACTTCATTTGTTACAAATCGATAAAACCACTTGTAGAGAGTGGGAAAATTGATCTACTATGA
Protein sequence

>MS.gene85342.t1

MGSRSTEGEQSSLADDYDALISTTDVELLKRAWRNEKAAPEILRFESDLINRVKGQIELMATFRKLEETVEEKLSVGSDPLSVSLYQMDLDRTLFLLRSYLRIRILKIEKYMFHIRKTQELWNRLSRDEKLFTERCTDDLKEHLESSVLSKLPENYQSYERQSVISEEDDMVPAPRLDTFVLCRSKEYLTGIQLEDGPVDDRSKLFEMEPDVLYFICYKSIKPLVESGKIDLL