Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065544.t1 | XP_003617613.1 | 84.6 | 228 | 32 | 3 | 1 | 226 | 1 | 227 | 5.90E-104 | 387.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065544.t1 | Q9SJ74 | 28.2 | 206 | 129 | 7 | 16 | 210 | 4 | 201 | 4.6e-12 | 73.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065544.t1 | G7K8L9 | 84.6 | 228 | 32 | 3 | 1 | 226 | 1 | 227 | 4.3e-104 | 387.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052732 | MS.gene065544 | 0.838291 | 3.14E-57 | -1.69E-46 |
| MS.gene053144 | MS.gene065544 | 0.818818 | 1.61E-52 | -1.69E-46 |
| MS.gene054584 | MS.gene065544 | 0.821287 | 4.37E-53 | -1.69E-46 |
| MS.gene055144 | MS.gene065544 | 0.841508 | 4.55E-58 | -1.69E-46 |
| MS.gene055469 | MS.gene065544 | 0.802522 | 5.39E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene065544 | 0.801107 | 1.05E-48 | -1.69E-46 |
| MS.gene055891 | MS.gene065544 | 0.805916 | 1.06E-49 | -1.69E-46 |
| MS.gene058339 | MS.gene065544 | 0.81566 | 8.25E-52 | -1.69E-46 |
| MS.gene058909 | MS.gene065544 | 0.80931 | 2.02E-50 | -1.69E-46 |
| MS.gene058912 | MS.gene065544 | 0.80396 | 2.72E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065544.t1 | MTR_5g093520 | 84.649 | 228 | 32 | 3 | 1 | 226 | 1 | 227 | 2.43e-134 | 385 |
| MS.gene065544.t1 | MTR_8g011650 | 27.642 | 246 | 153 | 10 | 6 | 242 | 1 | 230 | 3.43e-15 | 72.8 |
| MS.gene065544.t1 | MTR_7g051910 | 28.283 | 198 | 126 | 8 | 11 | 208 | 2 | 183 | 1.86e-13 | 69.3 |
| MS.gene065544.t1 | MTR_8g009880 | 28.700 | 223 | 137 | 9 | 16 | 226 | 1 | 213 | 2.82e-12 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene065544.t1 | AT2G04850 | 29.213 | 178 | 106 | 7 | 45 | 210 | 32 | 201 | 8.67e-12 | 64.7 |
| MS.gene065544.t1 | AT3G25290 | 28.834 | 163 | 103 | 6 | 50 | 208 | 42 | 195 | 5.09e-11 | 62.4 |
| MS.gene065544.t1 | AT3G25290 | 28.834 | 163 | 103 | 6 | 50 | 208 | 42 | 195 | 5.09e-11 | 62.4 |
Find 69 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAGTAGCAAAAGAGAAAA+AGG | 0.282441 | 5.1:+74602685 | None:intergenic |
| TTTCTCTTCTTTGCAAATAT+CGG | 0.320804 | 5.1:-74603311 | MS.gene065544:CDS |
| GCAAGTTGGGAAGGCTGTTA+GGG | 0.324027 | 5.1:-74602832 | MS.gene065544:CDS |
| GGCAAGTTGGGAAGGCTGTT+AGG | 0.324323 | 5.1:-74602833 | MS.gene065544:CDS |
| TGTTAGGGGTGATATACCTT+TGG | 0.324418 | 5.1:-74602817 | MS.gene065544:CDS |
| ACAATACTTGAAATCTTATT+CGG | 0.359172 | 5.1:-74603155 | MS.gene065544:CDS |
| TCTATATTGTGAATATTTGT+TGG | 0.359173 | 5.1:+74602789 | None:intergenic |
| CTGTCCCTTGCTGCGACCAT+CGG | 0.365418 | 5.1:+74602737 | None:intergenic |
| CCCATCCCCTTGATCCATAT+TGG | 0.365575 | 5.1:+74603130 | None:intergenic |
| AATCTTATTCGGTGCCAATA+TGG | 0.376709 | 5.1:-74603144 | MS.gene065544:CDS |
| ATTGGAACAAAGGCAATCAT+AGG | 0.380791 | 5.1:-74603065 | MS.gene065544:CDS |
| TAACAAGGTTGAACCATGTT+TGG | 0.384579 | 5.1:-74602854 | MS.gene065544:CDS |
| GTTAGGGGTGATATACCTTT+GGG | 0.389697 | 5.1:-74602816 | MS.gene065544:CDS |
| GCGGTAACTCCAACCGAATT+CGG | 0.389742 | 5.1:+74603196 | None:intergenic |
| ATCATTTCAGCCCTTTCCCC+TGG | 0.395994 | 5.1:+74603086 | None:intergenic |
| TAAAAGCCTACAACCCTGTT+TGG | 0.402063 | 5.1:+74602986 | None:intergenic |
| TGAACCATGTTTGGCAAGTT+GGG | 0.406735 | 5.1:-74602845 | MS.gene065544:CDS |
| CGAACTCTAGGAGCCGAATT+CGG | 0.413289 | 5.1:-74603209 | MS.gene065544:CDS |
| AAGGAACCAAACAGGGTTGT+AGG | 0.414976 | 5.1:-74602992 | MS.gene065544:CDS |
| GGTTCCTTTAGTAACATCAT+AGG | 0.424605 | 5.1:+74603007 | None:intergenic |
| GGGGAAAGGGCTGAAATGAT+TGG | 0.432198 | 5.1:-74603083 | MS.gene065544:CDS |
| TTGAACCATGTTTGGCAAGT+TGG | 0.443630 | 5.1:-74602846 | MS.gene065544:CDS |
| TGTTACTAAAGGAACCAAAC+AGG | 0.445031 | 5.1:-74603000 | MS.gene065544:CDS |
| CACGTTAAAGCCAATATCAT+TGG | 0.452463 | 5.1:+74602959 | None:intergenic |
| GAGAAAGGACAATATCATAA+TGG | 0.453788 | 5.1:+74603328 | None:intergenic |
| TGTCCCTTGCTGCGACCATC+GGG | 0.455324 | 5.1:+74602738 | None:intergenic |
| GGGTGTAAATCCAGGGGAAA+GGG | 0.460004 | 5.1:-74603096 | MS.gene065544:CDS |
| CTCTAGGAGCCGAATTCGGT+TGG | 0.468915 | 5.1:-74603205 | MS.gene065544:CDS |
| AAGGGGATGGGTGGATAGCA+TGG | 0.470518 | 5.1:-74603118 | MS.gene065544:CDS |
| ATAGCATGGGGTGTAAATCC+AGG | 0.477364 | 5.1:-74603104 | MS.gene065544:CDS |
| GCCAATATGGATCAAGGGGA+TGG | 0.480674 | 5.1:-74603131 | MS.gene065544:CDS |
| TTCGGTGCCAATATGGATCA+AGG | 0.487774 | 5.1:-74603137 | MS.gene065544:CDS |
| CAATATCATAATGGTTACTA+AGG | 0.488331 | 5.1:+74603337 | None:intergenic |
| GATATGTCGATTCAAGATGA+AGG | 0.502397 | 5.1:-74602933 | MS.gene065544:CDS |
| AGGGGATGGGTGGATAGCAT+GGG | 0.510652 | 5.1:-74603117 | MS.gene065544:CDS |
| TATTGTGAATATTTGTTGGA+TGG | 0.512465 | 5.1:+74602793 | None:intergenic |
| GTTATGTTATATTTATCAGA+AGG | 0.514306 | 5.1:+74602873 | None:intergenic |
| CCTTCCCAACTTGCCAAACA+TGG | 0.528715 | 5.1:+74602841 | None:intergenic |
| GGGGTGTAAATCCAGGGGAA+AGG | 0.530797 | 5.1:-74603097 | MS.gene065544:CDS |
| GAATAAGATTTCAAGTATTG+TGG | 0.534057 | 5.1:+74603157 | None:intergenic |
| TTATCGCTGCATGGTTGTTG+AGG | 0.534691 | 5.1:+74603281 | None:intergenic |
| AAAACCTATGATGTTACTAA+AGG | 0.534733 | 5.1:-74603011 | MS.gene065544:CDS |
| ATCATAGGGATCAAATATCA+TGG | 0.537405 | 5.1:-74603050 | MS.gene065544:CDS |
| GAAACTGAGTTTCCTTCGAT+CGG | 0.542803 | 5.1:-74602715 | MS.gene065544:CDS |
| GATATTTGCAAAGAAGAGAA+AGG | 0.546213 | 5.1:+74603313 | None:intergenic |
| GGCTGAAATGATTGGAACAA+AGG | 0.548087 | 5.1:-74603075 | MS.gene065544:CDS |
| CCAATATGGATCAAGGGGAT+GGG | 0.551351 | 5.1:-74603130 | MS.gene065544:CDS |
| TGCAAAACATTACGAACTCT+AGG | 0.556387 | 5.1:-74603221 | MS.gene065544:CDS |
| TTCGCGAAGTTATCGCTGCA+TGG | 0.557211 | 5.1:+74603272 | None:intergenic |
| ATATGGATCAAGGGGATGGG+TGG | 0.557364 | 5.1:-74603127 | MS.gene065544:CDS |
| CTGATAAATATAACATAACA+AGG | 0.566580 | 5.1:-74602869 | MS.gene065544:CDS |
| TTCTCCCGATGGTCGCAGCA+AGG | 0.567113 | 5.1:-74602742 | MS.gene065544:CDS |
| TCTCCCGATGGTCGCAGCAA+GGG | 0.568984 | 5.1:-74602741 | MS.gene065544:CDS |
| TAGCATGGGGTGTAAATCCA+GGG | 0.574940 | 5.1:-74603103 | MS.gene065544:CDS |
| TCGGTGCCAATATGGATCAA+GGG | 0.576863 | 5.1:-74603136 | MS.gene065544:CDS |
| CCATGTTTGGCAAGTTGGGA+AGG | 0.580201 | 5.1:-74602841 | MS.gene065544:CDS |
| AAAGGATTGTTACCGATCGA+AGG | 0.580655 | 5.1:+74602703 | None:intergenic |
| GGGGATGGGTGGATAGCATG+GGG | 0.582355 | 5.1:-74603116 | MS.gene065544:CDS |
| TTATGTTATATTTATCAGAA+GGG | 0.582589 | 5.1:+74602874 | None:intergenic |
| TTGGAACAAAGGCAATCATA+GGG | 0.588140 | 5.1:-74603064 | MS.gene065544:CDS |
| ATATTTGTTGGATGGCCCAA+AGG | 0.598917 | 5.1:+74602801 | None:intergenic |
| CGGTGCCAATATGGATCAAG+GGG | 0.603107 | 5.1:-74603135 | MS.gene065544:CDS |
| CAAGTTGGGAAGGCTGTTAG+GGG | 0.623437 | 5.1:-74602831 | MS.gene065544:CDS |
| ATATGTCGATTCAAGATGAA+GGG | 0.624440 | 5.1:-74602932 | MS.gene065544:CDS |
| TGGTTGAGTTTGTGCCGTTG+CGG | 0.633100 | 5.1:+74603177 | None:intergenic |
| GTTACTAAAGGAACCAAACA+GGG | 0.664990 | 5.1:-74602999 | MS.gene065544:CDS |
| TTCGGTTGGAGTTACCGCAA+CGG | 0.679084 | 5.1:-74603191 | MS.gene065544:CDS |
| ATAGACTTGACTTCTCCCGA+TGG | 0.700298 | 5.1:-74602753 | MS.gene065544:CDS |
| AGCATGGGGTGTAAATCCAG+GGG | 0.707308 | 5.1:-74603102 | MS.gene065544:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATGTTATATTTATCAGAA+GGG | + | chr5.1:74603159-74603178 | None:intergenic | 15.0% |
| !! | ACAATACTTGAAATCTTATT+CGG | - | chr5.1:74602875-74602894 | MS.gene065544:CDS | 20.0% |
| !! | CTGATAAATATAACATAACA+AGG | - | chr5.1:74603161-74603180 | MS.gene065544:CDS | 20.0% |
| !! | GTTATGTTATATTTATCAGA+AGG | + | chr5.1:74603160-74603179 | None:intergenic | 20.0% |
| !! | TCTATATTGTGAATATTTGT+TGG | + | chr5.1:74603244-74603263 | None:intergenic | 20.0% |
| ! | AAAACCTATGATGTTACTAA+AGG | - | chr5.1:74603019-74603038 | MS.gene065544:CDS | 25.0% |
| ! | CAATATCATAATGGTTACTA+AGG | + | chr5.1:74602696-74602715 | None:intergenic | 25.0% |
| ! | TTTCTCTTCTTTGCAAATAT+CGG | - | chr5.1:74602719-74602738 | MS.gene065544:CDS | 25.0% |
| !! | CTTTTATCTACCAATGATAT+TGG | - | chr5.1:74603061-74603080 | MS.gene065544:CDS | 25.0% |
| !! | GAATAAGATTTCAAGTATTG+TGG | + | chr5.1:74602876-74602895 | None:intergenic | 25.0% |
| !! | TATTGTGAATATTTGTTGGA+TGG | + | chr5.1:74603240-74603259 | None:intergenic | 25.0% |
| ATCATAGGGATCAAATATCA+TGG | - | chr5.1:74602980-74602999 | MS.gene065544:CDS | 30.0% | |
| CATAGTAGCAAAAGAGAAAA+AGG | + | chr5.1:74603348-74603367 | None:intergenic | 30.0% | |
| GAGAAAGGACAATATCATAA+TGG | + | chr5.1:74602705-74602724 | None:intergenic | 30.0% | |
| GATATTTGCAAAGAAGAGAA+AGG | + | chr5.1:74602720-74602739 | None:intergenic | 30.0% | |
| !! | ATATGTCGATTCAAGATGAA+GGG | - | chr5.1:74603098-74603117 | MS.gene065544:CDS | 30.0% |
| !! | GTAACATCATAGGTTTTAAC+TGG | + | chr5.1:74603016-74603035 | None:intergenic | 30.0% |
| ATTGGAACAAAGGCAATCAT+AGG | - | chr5.1:74602965-74602984 | MS.gene065544:CDS | 35.0% | |
| CACGTTAAAGCCAATATCAT+TGG | + | chr5.1:74603074-74603093 | None:intergenic | 35.0% | |
| GGTTCCTTTAGTAACATCAT+AGG | + | chr5.1:74603026-74603045 | None:intergenic | 35.0% | |
| GTTACTAAAGGAACCAAACA+GGG | - | chr5.1:74603031-74603050 | MS.gene065544:CDS | 35.0% | |
| TAACAAGGTTGAACCATGTT+TGG | - | chr5.1:74603176-74603195 | MS.gene065544:CDS | 35.0% | |
| TGCAAAACATTACGAACTCT+AGG | - | chr5.1:74602809-74602828 | MS.gene065544:CDS | 35.0% | |
| TGTTACTAAAGGAACCAAAC+AGG | - | chr5.1:74603030-74603049 | MS.gene065544:CDS | 35.0% | |
| TTGGAACAAAGGCAATCATA+GGG | - | chr5.1:74602966-74602985 | MS.gene065544:CDS | 35.0% | |
| !! | AATCTTATTCGGTGCCAATA+TGG | - | chr5.1:74602886-74602905 | MS.gene065544:CDS | 35.0% |
| !! | GATATGTCGATTCAAGATGA+AGG | - | chr5.1:74603097-74603116 | MS.gene065544:CDS | 35.0% |
| AAAGGATTGTTACCGATCGA+AGG | + | chr5.1:74603330-74603349 | None:intergenic | 40.0% | |
| GAAACTGAGTTTCCTTCGAT+CGG | - | chr5.1:74603315-74603334 | MS.gene065544:CDS | 40.0% | |
| GGCTGAAATGATTGGAACAA+AGG | - | chr5.1:74602955-74602974 | MS.gene065544:CDS | 40.0% | |
| GTTAGGGGTGATATACCTTT+GGG | - | chr5.1:74603214-74603233 | MS.gene065544:CDS | 40.0% | |
| TAAAAGCCTACAACCCTGTT+TGG | + | chr5.1:74603047-74603066 | None:intergenic | 40.0% | |
| TGAACCATGTTTGGCAAGTT+GGG | - | chr5.1:74603185-74603204 | MS.gene065544:CDS | 40.0% | |
| TGTTAGGGGTGATATACCTT+TGG | - | chr5.1:74603213-74603232 | MS.gene065544:CDS | 40.0% | |
| TTGAACCATGTTTGGCAAGT+TGG | - | chr5.1:74603184-74603203 | MS.gene065544:CDS | 40.0% | |
| ! | ATATTTGTTGGATGGCCCAA+AGG | + | chr5.1:74603232-74603251 | None:intergenic | 40.0% |
| AAGGAACCAAACAGGGTTGT+AGG | - | chr5.1:74603038-74603057 | MS.gene065544:CDS | 45.0% | |
| ATAGACTTGACTTCTCCCGA+TGG | - | chr5.1:74603277-74603296 | MS.gene065544:CDS | 45.0% | |
| ATAGCATGGGGTGTAAATCC+AGG | - | chr5.1:74602926-74602945 | MS.gene065544:CDS | 45.0% | |
| CCAATATGGATCAAGGGGAT+GGG | - | chr5.1:74602900-74602919 | MS.gene065544:CDS | 45.0% | |
| TAGCATGGGGTGTAAATCCA+GGG | - | chr5.1:74602927-74602946 | MS.gene065544:CDS | 45.0% | |
| TTATCGCTGCATGGTTGTTG+AGG | + | chr5.1:74602752-74602771 | None:intergenic | 45.0% | |
| !! | TCGGTGCCAATATGGATCAA+GGG | - | chr5.1:74602894-74602913 | MS.gene065544:CDS | 45.0% |
| !! | TTCGGTGCCAATATGGATCA+AGG | - | chr5.1:74602893-74602912 | MS.gene065544:CDS | 45.0% |
| AGCATGGGGTGTAAATCCAG+GGG | - | chr5.1:74602928-74602947 | MS.gene065544:CDS | 50.0% | |
| ATATGGATCAAGGGGATGGG+TGG | - | chr5.1:74602903-74602922 | MS.gene065544:CDS | 50.0% | |
| ATCATTTCAGCCCTTTCCCC+TGG | + | chr5.1:74602947-74602966 | None:intergenic | 50.0% | |
| CCCATCCCCTTGATCCATAT+TGG | + | chr5.1:74602903-74602922 | None:intergenic | 50.0% | |
| CCTTCCCAACTTGCCAAACA+TGG | + | chr5.1:74603192-74603211 | None:intergenic | 50.0% | |
| GCCAATATGGATCAAGGGGA+TGG | - | chr5.1:74602899-74602918 | MS.gene065544:CDS | 50.0% | |
| GCGGTAACTCCAACCGAATT+CGG | + | chr5.1:74602837-74602856 | None:intergenic | 50.0% | |
| GGGGAAAGGGCTGAAATGAT+TGG | - | chr5.1:74602947-74602966 | MS.gene065544:CDS | 50.0% | |
| GGGTGTAAATCCAGGGGAAA+GGG | - | chr5.1:74602934-74602953 | MS.gene065544:CDS | 50.0% | |
| TTCGCGAAGTTATCGCTGCA+TGG | + | chr5.1:74602761-74602780 | None:intergenic | 50.0% | |
| TTCGGTTGGAGTTACCGCAA+CGG | - | chr5.1:74602839-74602858 | MS.gene065544:CDS | 50.0% | |
| ! | CAAGTTGGGAAGGCTGTTAG+GGG | - | chr5.1:74603199-74603218 | MS.gene065544:CDS | 50.0% |
| ! | CCATGTTTGGCAAGTTGGGA+AGG | - | chr5.1:74603189-74603208 | MS.gene065544:CDS | 50.0% |
| ! | CGAACTCTAGGAGCCGAATT+CGG | - | chr5.1:74602821-74602840 | MS.gene065544:CDS | 50.0% |
| ! | CGGTGCCAATATGGATCAAG+GGG | - | chr5.1:74602895-74602914 | MS.gene065544:CDS | 50.0% |
| ! | GCAAGTTGGGAAGGCTGTTA+GGG | - | chr5.1:74603198-74603217 | MS.gene065544:CDS | 50.0% |
| ! | TGGTTGAGTTTGTGCCGTTG+CGG | + | chr5.1:74602856-74602875 | None:intergenic | 50.0% |
| AAGGGGATGGGTGGATAGCA+TGG | - | chr5.1:74602912-74602931 | MS.gene065544:CDS | 55.0% | |
| AGGGGATGGGTGGATAGCAT+GGG | - | chr5.1:74602913-74602932 | MS.gene065544:CDS | 55.0% | |
| GGGGTGTAAATCCAGGGGAA+AGG | - | chr5.1:74602933-74602952 | MS.gene065544:CDS | 55.0% | |
| ! | CTCTAGGAGCCGAATTCGGT+TGG | - | chr5.1:74602825-74602844 | MS.gene065544:CDS | 55.0% |
| ! | GGCAAGTTGGGAAGGCTGTT+AGG | - | chr5.1:74603197-74603216 | MS.gene065544:CDS | 55.0% |
| CTGTCCCTTGCTGCGACCAT+CGG | + | chr5.1:74603296-74603315 | None:intergenic | 60.0% | |
| GGGGATGGGTGGATAGCATG+GGG | - | chr5.1:74602914-74602933 | MS.gene065544:CDS | 60.0% | |
| TCTCCCGATGGTCGCAGCAA+GGG | - | chr5.1:74603289-74603308 | MS.gene065544:CDS | 60.0% | |
| TGTCCCTTGCTGCGACCATC+GGG | + | chr5.1:74603295-74603314 | None:intergenic | 60.0% | |
| TTCTCCCGATGGTCGCAGCA+AGG | - | chr5.1:74603288-74603307 | MS.gene065544:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 74602662 | 74603390 | 74602662 | ID=MS.gene065544 |
| chr5.1 | mRNA | 74602662 | 74603390 | 74602662 | ID=MS.gene065544.t1;Parent=MS.gene065544 |
| chr5.1 | exon | 74602662 | 74603390 | 74602662 | ID=MS.gene065544.t1.exon1;Parent=MS.gene065544.t1 |
| chr5.1 | CDS | 74602662 | 74603390 | 74602662 | ID=cds.MS.gene065544.t1;Parent=MS.gene065544.t1 |
| Gene Sequence |
| Protein sequence |
