AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065968

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065968.t1 MTR_8g087540 97.257 401 11 0 1 401 1 401 0.0 814
MS.gene065968.t1 MTR_2g017890 40.633 411 186 9 1 383 2 382 6.88e-87 269
MS.gene065968.t1 MTR_6g043060 36.212 359 177 9 6 358 16 328 3.69e-63 206
MS.gene065968.t1 MTR_5g029680 51.630 184 82 1 11 187 31 214 1.13e-60 204
MS.gene065968.t1 MTR_8g083100 52.239 201 84 4 13 207 23 217 1.94e-60 203
MS.gene065968.t1 MTR_2g100680 49.751 201 92 2 12 207 31 227 3.18e-60 202
MS.gene065968.t1 MTR_2g100670 49.744 195 86 2 1 187 14 204 4.41e-54 182
MS.gene065968.t1 MTR_2g100670 49.744 195 86 2 1 187 122 312 5.61e-53 182
MS.gene065968.t1 MTR_1g102860 36.082 291 166 4 11 288 39 322 1.10e-52 179
MS.gene065968.t1 MTR_4g077970 44.865 185 94 2 10 187 10 193 1.50e-51 180
MS.gene065968.t1 MTR_7g095680 39.130 230 126 3 11 226 48 277 9.09e-49 169
MS.gene065968.t1 MTR_3g104550 41.584 202 114 1 11 208 45 246 1.09e-46 164
MS.gene065968.t1 MTR_1g061170 41.935 186 106 1 8 193 45 228 3.55e-46 165
MS.gene065968.t1 MTR_8g105780 46.875 192 91 3 12 194 22 211 1.12e-45 161
MS.gene065968.t1 MTR_1g071360 42.541 181 87 3 12 189 32 198 2.17e-43 154
MS.gene065968.t1 MTR_1g071360 42.541 181 87 3 12 189 29 195 2.86e-43 155
MS.gene065968.t1 MTR_2g043940 46.995 183 80 4 52 229 11 181 1.75e-41 150
MS.gene065968.t1 MTR_6g086805 38.308 201 116 2 10 206 9 205 4.38e-41 148
MS.gene065968.t1 MTR_5g010680 64.356 101 35 1 4 103 15 115 8.21e-41 148
MS.gene065968.t1 MTR_3g101870 64.646 99 34 1 6 103 17 115 2.77e-39 144
MS.gene065968.t1 MTR_5g089170 66.667 99 32 1 6 103 18 116 8.50e-39 140
MS.gene065968.t1 MTR_5g017470 58.824 102 42 0 8 109 5 106 1.00e-38 140
MS.gene065968.t1 MTR_1g100777 58.163 98 41 0 8 105 14 111 3.63e-37 137
MS.gene065968.t1 MTR_0020s0020 59.596 99 39 1 6 103 26 124 7.82e-36 134
MS.gene065968.t1 MTR_7g091370 49.123 114 58 0 3 116 13 126 2.74e-34 127
MS.gene065968.t1 MTR_4g088900 37.714 175 91 5 12 176 33 199 7.11e-30 115
MS.gene065968.t1 MTR_5g047190 53.061 98 39 2 20 110 1 98 1.86e-27 105
MS.gene065968.t1 MTR_4g045757 63.333 60 22 0 10 69 12 71 1.29e-21 88.6
MS.gene065968.t1 MTR_5g022830 87.805 41 5 0 361 401 40 80 5.13e-19 81.3
MS.gene065968.t1 MTR_6g044340 28.571 308 148 15 64 358 14 262 2.40e-17 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065968.t1 AT4G18880 51.095 411 179 11 1 400 1 400 3.98e-115 342
MS.gene065968.t1 AT5G45710 49.492 394 145 8 1 391 1 343 2.30e-110 328
MS.gene065968.t1 AT5G45710 49.492 394 145 8 1 391 1 343 2.30e-110 328
MS.gene065968.t1 AT4G13980 48.500 200 94 2 13 207 24 219 2.28e-59 200
MS.gene065968.t1 AT1G32330 41.949 236 118 2 6 222 31 266 1.24e-58 198
MS.gene065968.t1 AT1G32330 41.949 236 118 2 6 222 31 266 1.53e-58 198
MS.gene065968.t1 AT5G45710 40.940 298 123 8 96 391 1 247 1.77e-55 183
MS.gene065968.t1 AT5G45710 40.940 298 123 8 96 391 1 247 1.77e-55 183
MS.gene065968.t1 AT5G16820 42.991 214 109 2 8 208 23 236 1.45e-54 187
MS.gene065968.t1 AT5G16820 42.991 214 109 2 8 208 23 236 1.45e-54 187
MS.gene065968.t1 AT4G17750 45.026 191 91 1 11 187 51 241 7.66e-54 186
MS.gene065968.t1 AT3G22830 41.256 223 117 2 8 216 57 279 1.92e-53 183
MS.gene065968.t1 AT3G02990 47.283 184 93 1 8 187 19 202 3.84e-53 184
MS.gene065968.t1 AT2G26150 41.262 206 113 1 11 208 43 248 2.28e-50 173
MS.gene065968.t1 AT2G26150 41.262 206 113 1 11 208 43 248 2.28e-50 173
MS.gene065968.t1 AT5G03720 45.810 179 89 2 10 185 53 226 7.43e-49 171
MS.gene065968.t1 AT5G03720 45.810 179 89 2 10 185 53 226 7.43e-49 171
MS.gene065968.t1 AT5G43840 43.000 200 90 3 11 208 16 193 1.36e-46 161
MS.gene065968.t1 AT1G67970 43.386 189 100 3 10 194 17 202 1.71e-45 161
MS.gene065968.t1 AT3G51910 45.652 184 85 3 11 194 28 196 4.30e-44 154
MS.gene065968.t1 AT5G54070 39.698 199 110 2 10 208 69 257 3.41e-41 148
MS.gene065968.t1 AT3G24520 39.247 186 86 4 10 195 15 173 1.69e-40 147
MS.gene065968.t1 AT1G46264 63.000 100 36 1 5 103 26 125 1.78e-39 144
MS.gene065968.t1 AT3G63350 38.725 204 111 3 1 204 21 210 1.90e-39 142
MS.gene065968.t1 AT5G62020 51.181 127 57 2 3 128 13 135 2.08e-38 140
MS.gene065968.t1 AT4G36990 63.265 98 35 1 9 105 10 107 9.05e-38 138
MS.gene065968.t1 AT4G11660 59.596 99 39 1 6 103 52 150 1.64e-36 137
MS.gene065968.t1 AT2G41690 58.491 106 43 1 11 115 39 144 2.19e-36 133
MS.gene065968.t1 AT4G18870 54.639 97 39 2 5 99 6 99 6.27e-27 109
MS.gene065968.t1 AT4G18870 52.174 92 44 0 8 99 142 233 2.05e-26 107
MS.gene065968.t1 AT1G77570 43.333 90 48 2 13 99 27 116 1.45e-17 79.3
MS.gene065968.t1 AT2G26150 33.621 116 77 0 72 187 49 164 1.14e-16 80.1
MS.gene065968.t1 AT2G26150 33.621 116 77 0 72 187 49 164 1.14e-16 80.1
MS.gene065968.t1 AT4G19630 38.947 95 55 1 8 99 7 101 2.15e-15 72.8

Find 84 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGGCATCCGTGGCATTT+TGG 0.204494 4.3:+13373392 MS.gene065968:CDS
TCAACGTTCATGTCAATTTC+AGG 0.245219 4.3:-13373550 None:intergenic
ATATGTATGTGCAGGGATTT+AGG 0.272285 4.3:+13372843 MS.gene065968:intron
AGCAAAACCCTTGCGAATTC+CGG 0.278319 4.3:-13372657 None:intergenic
CACTCATTCAAACATGGATT+TGG 0.293073 4.3:+13373453 MS.gene065968:CDS
CTGGGGTTAATGATGTATTC+TGG 0.294292 4.3:+13373641 MS.gene065968:CDS
CTAACATGCCCCATCTGTTC+TGG 0.312124 4.3:-13373808 None:intergenic
CGAAGAGATACCAGCATGAT+TGG 0.339945 4.3:+13373077 MS.gene065968:CDS
CTAGTGATCATGGTAGATTT+TGG 0.344391 4.3:+13373758 MS.gene065968:CDS
AGAATCACCACTGCAGCAAC+TGG 0.356379 4.3:+13373622 MS.gene065968:CDS
AAGAGATTTACAGCAATCAT+AGG 0.368528 4.3:-13373202 None:intergenic
GACGTTAAACACCGAGAGAT+TGG 0.369357 4.3:+13373363 MS.gene065968:CDS
TGCCTTTCAGACTCCGTTAG+AGG 0.391700 4.3:-13372995 None:intergenic
GGAGAGAAAACGAAGGTTGC+CGG 0.399062 4.3:+13373243 MS.gene065968:CDS
ATTCAGCTGCCTGATAAAAC+TGG 0.407135 4.3:-13372709 None:intergenic
CTCTCCATGGCTTCTGCCAG+TGG 0.418903 4.3:-13373226 None:intergenic
TCATAAGTCTTTGCCAGAAA+AGG 0.419332 4.3:-13372558 None:intergenic
GCCTCAAGAGCAGCAGCAGC+TGG 0.427478 4.3:-13373574 None:intergenic
TCTGGCAAAGACTTATGAAA+TGG 0.438206 4.3:+13372563 MS.gene065968:CDS
TGAAATAGAGAAGCTGAAAC+AGG 0.448407 4.3:+13373030 MS.gene065968:CDS
GATGCACTCCACGAAACAAT+AGG 0.454683 4.3:-13372606 None:intergenic
TGGGGTTAATGATGTATTCT+GGG 0.456218 4.3:+13373642 MS.gene065968:CDS
ACCAGCTGCTGCTGCTCTTG+AGG 0.466489 4.3:+13373573 MS.gene065968:CDS
GCCTTTCAGACTCCGTTAGA+GGG 0.473627 4.3:-13372994 None:intergenic
ATGTCAATTTCAGGTGACTT+AGG 0.477806 4.3:-13373541 None:intergenic
TGCAGAGAATGACTATGAAC+GGG 0.482637 4.3:-13372953 None:intergenic
TCCTGGTGCATCGGAAGCAC+AGG 0.486616 4.3:+13373684 MS.gene065968:CDS
GAGACTCTTGCACATGAAGT+TGG 0.497289 4.3:+13373415 MS.gene065968:CDS
CGTTCACACTCATTCAAACA+TGG 0.502442 4.3:+13373447 MS.gene065968:CDS
GAAGGAAAACCTAGTGATCA+TGG 0.502730 4.3:+13373748 MS.gene065968:CDS
TTGGCAAATTCCCACTGCTC+CGG 0.502904 4.3:-13372875 None:intergenic
GGGATTTAGGAAAGTTGATC+CGG 0.507404 4.3:+13372856 MS.gene065968:intron
CTTGCCACTGGCAGAAGCCA+TGG 0.509175 4.3:+13373222 MS.gene065968:CDS
TTTCGTTGTGTGGAATCCTC+CGG 0.511600 4.3:+13372638 MS.gene065968:CDS
TCCTGTGCTTCCGATGCACC+AGG 0.511617 4.3:-13373685 None:intergenic
GAAGAGATACCAGCATGATT+GGG 0.512133 4.3:+13373078 MS.gene065968:CDS
TGCTGTAAATCTCTTGCCAC+TGG 0.515181 4.3:+13373210 MS.gene065968:CDS
GGAAAGTTGATCCGGAGCAG+TGG 0.515618 4.3:+13372864 MS.gene065968:CDS
CAGTTCTTGACAGAGGATCC+TGG 0.516203 4.3:+13373667 MS.gene065968:CDS
AGACTTCTGTAGCTTTGCCC+AGG 0.516910 4.3:+13373314 MS.gene065968:CDS
AAACAATCATCCAGAACAGA+TGG 0.518388 4.3:+13373798 MS.gene065968:CDS
CCTCGGATCCTATTGTTTCG+TGG 0.527360 4.3:+13372598 MS.gene065968:CDS
TTATTGTGCTTAAAGAATCT+CGG 0.532647 4.3:-13372681 None:intergenic
CGAACAAAAGTTTCGTTGTG+TGG 0.533195 4.3:+13372628 MS.gene065968:CDS
GCATTGTTCGAAAGATCAGT+TGG 0.538254 4.3:+13373120 MS.gene065968:CDS
GTCAAAGTAGAGTTATCCTC+TGG 0.546841 4.3:-13373343 None:intergenic
AGCTAGTGTTGAAGATGCTA+TGG 0.547413 4.3:+13373291 MS.gene065968:CDS
CTCAAGTTGATCCAATCTCT+CGG 0.553126 4.3:-13373374 None:intergenic
GACAGAGGATCCTGGTGCAT+CGG 0.553354 4.3:+13373675 MS.gene065968:CDS
ATTAACCCCAGTTGCTGCAG+TGG 0.553513 4.3:-13373629 None:intergenic
CCCCTCTAACGGAGTCTGAA+AGG 0.554429 4.3:+13372993 MS.gene065968:CDS
ATGCAGAGAATGACTATGAA+CGG 0.560960 4.3:-13372954 None:intergenic
GCTGCAGTGGTGATTCTCGC+AGG 0.569378 4.3:-13373616 None:intergenic
TAAGTCTTTGCCAGAAAAGG+AGG 0.570699 4.3:-13372555 None:intergenic
CAAAATCTACCATGATCACT+AGG 0.571873 4.3:-13373757 None:intergenic
AACAATCATCCAGAACAGAT+GGG 0.574845 4.3:+13373799 MS.gene065968:CDS
GGAATCCTCCGGAATTCGCA+AGG 0.575730 4.3:+13372649 MS.gene065968:CDS
TTAAAGCAACCACTTCTCGC+CGG 0.577237 4.3:-13373262 None:intergenic
GAAAGTTGATCCGGAGCAGT+GGG 0.578322 4.3:+13372865 MS.gene065968:CDS
CATGCCTTTCCCAATCATGC+TGG 0.579073 4.3:-13373087 None:intergenic
GAATCACCACTGCAGCAACT+GGG 0.580374 4.3:+13373623 MS.gene065968:CDS
CGAAGGTTGCCGGCGAGAAG+TGG 0.586677 4.3:+13373253 MS.gene065968:CDS
CCTTTCAGACTCCGTTAGAG+GGG 0.598591 4.3:-13372993 None:intergenic
TGAATATTCTTCATCAGATG+CGG 0.602613 4.3:-13372920 None:intergenic
GACTTCTGTAGCTTTGCCCA+GGG 0.603526 4.3:+13373315 MS.gene065968:CDS
GATACCAGCATGATTGGGAA+AGG 0.606022 4.3:+13373083 MS.gene065968:CDS
AAAACCCTTGCGAATTCCGG+AGG 0.607238 4.3:-13372654 None:intergenic
TCAAGCACAAGCCCCTCTAA+CGG 0.613167 4.3:+13372982 MS.gene065968:CDS
GGCAAAGACTTATGAAATGG+TGG 0.613237 4.3:+13372566 MS.gene065968:CDS
GGATCAACTTGAGGCATCCG+TGG 0.615719 4.3:+13373384 MS.gene065968:CDS
CGAGAGATTGGATCAACTTG+AGG 0.627773 4.3:+13373375 MS.gene065968:CDS
AATGGTGGATGATCGATCCT+CGG 0.628991 4.3:+13372581 MS.gene065968:CDS
GAATCCTCCGGAATTCGCAA+GGG 0.629052 4.3:+13372650 MS.gene065968:CDS
TGATGAAGAATATTCATAGA+CGG 0.629516 4.3:+13372927 MS.gene065968:CDS
ATATCTTGTCAGCAACTAGA+CGG 0.636770 4.3:+13373508 MS.gene065968:CDS
ACAATCATCCAGAACAGATG+GGG 0.647372 4.3:+13373800 MS.gene065968:CDS
CTGGGAACAGTTCTTGACAG+AGG 0.655854 4.3:+13373660 MS.gene065968:CDS
AAGAGTCTCCCAAAATGCCA+CGG 0.657362 4.3:-13373401 None:intergenic
AATCACCACTGCAGCAACTG+GGG 0.658502 4.3:+13373624 MS.gene065968:CDS
GGATAGAGAACAACTCCTCG+TGG 0.661247 4.3:+13373051 MS.gene065968:CDS
CCACGAAACAATAGGATCCG+AGG 0.666703 4.3:-13372598 None:intergenic
GGTATCTCTTCGTCTCCACG+AGG 0.715796 4.3:-13373066 None:intergenic
TTTGCCCAGGGAAAATCCAG+AGG 0.715905 4.3:+13373327 MS.gene065968:CDS
AAGCCATGGAGAGAAAACGA+AGG 0.761326 4.3:+13373236 MS.gene065968:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTATTTACTGATTC+TGG + chr4.3:13372792-13372811 MS.gene065968:intron 15.0%
! AATAGTAGCAGAAAAAATGA+AGG + chr4.3:13373730-13373749 MS.gene065968:CDS 25.0%
! TATGTAAGCAAATTTGATGT+CGG + chr4.3:13372735-13372754 MS.gene065968:intron 25.0%
! TGATGAAGAATATTCATAGA+CGG + chr4.3:13372927-13372946 MS.gene065968:CDS 25.0%
! TTATTGTGCTTAAAGAATCT+CGG - chr4.3:13372684-13372703 None:intergenic 25.0%
!! TGCCAATGATGATTTTATAA+GGG + chr4.3:13372892-13372911 MS.gene065968:CDS 25.0%
!! TTGCCAATGATGATTTTATA+AGG + chr4.3:13372891-13372910 MS.gene065968:CDS 25.0%
!!! TATTTACTGATTCTGGTTTT+TGG + chr4.3:13372799-13372818 MS.gene065968:intron 25.0%
AAGAGATTTACAGCAATCAT+AGG - chr4.3:13373205-13373224 None:intergenic 30.0%
GAAAGATCAGTTGGAAAAAT+TGG + chr4.3:13373129-13373148 MS.gene065968:CDS 30.0%
TCATAGTATATGTATGTGCA+GGG + chr4.3:13372836-13372855 MS.gene065968:intron 30.0%
TGAATATTCTTCATCAGATG+CGG - chr4.3:13372923-13372942 None:intergenic 30.0%
TTCATAGTATATGTATGTGC+AGG + chr4.3:13372835-13372854 MS.gene065968:intron 30.0%
! CCAATGATGATTTTATAAGG+GGG + chr4.3:13372894-13372913 MS.gene065968:CDS 30.0%
! GCCAATGATGATTTTATAAG+GGG + chr4.3:13372893-13372912 MS.gene065968:CDS 30.0%
!! ATAACTTCTCCAGTTTTATC+AGG + chr4.3:13372700-13372719 MS.gene065968:CDS 30.0%
AAACAATCATCCAGAACAGA+TGG + chr4.3:13373798-13373817 MS.gene065968:CDS 35.0%
AACAATCATCCAGAACAGAT+GGG + chr4.3:13373799-13373818 MS.gene065968:CDS 35.0%
ATATCTTGTCAGCAACTAGA+CGG + chr4.3:13373508-13373527 MS.gene065968:CDS 35.0%
ATATGTATGTGCAGGGATTT+AGG + chr4.3:13372843-13372862 MS.gene065968:intron 35.0%
ATGCAGAGAATGACTATGAA+CGG - chr4.3:13372957-13372976 None:intergenic 35.0%
CAAAATCTACCATGATCACT+AGG - chr4.3:13373760-13373779 None:intergenic 35.0%
CACTCATTCAAACATGGATT+TGG + chr4.3:13373453-13373472 MS.gene065968:CDS 35.0%
CCCCCTTATAAAATCATCAT+TGG - chr4.3:13372897-13372916 None:intergenic 35.0%
TCAACGTTCATGTCAATTTC+AGG - chr4.3:13373553-13373572 None:intergenic 35.0%
TCATAAGTCTTTGCCAGAAA+AGG - chr4.3:13372561-13372580 None:intergenic 35.0%
TCTGGCAAAGACTTATGAAA+TGG + chr4.3:13372563-13372582 MS.gene065968:CDS 35.0%
TGAAATAGAGAAGCTGAAAC+AGG + chr4.3:13373030-13373049 MS.gene065968:CDS 35.0%
TGGGGTTAATGATGTATTCT+GGG + chr4.3:13373642-13373661 MS.gene065968:CDS 35.0%
! ATGTCAATTTCAGGTGACTT+AGG - chr4.3:13373544-13373563 None:intergenic 35.0%
! CTAGTGATCATGGTAGATTT+TGG + chr4.3:13373758-13373777 MS.gene065968:CDS 35.0%
ACAATCATCCAGAACAGATG+GGG + chr4.3:13373800-13373819 MS.gene065968:CDS 40.0%
ATTCAGCTGCCTGATAAAAC+TGG - chr4.3:13372712-13372731 None:intergenic 40.0%
CGAACAAAAGTTTCGTTGTG+TGG + chr4.3:13372628-13372647 MS.gene065968:CDS 40.0%
CGTTCACACTCATTCAAACA+TGG + chr4.3:13373447-13373466 MS.gene065968:CDS 40.0%
CTGGGGTTAATGATGTATTC+TGG + chr4.3:13373641-13373660 MS.gene065968:CDS 40.0%
GAAGAGATACCAGCATGATT+GGG + chr4.3:13373078-13373097 MS.gene065968:CDS 40.0%
GAAGGAAAACCTAGTGATCA+TGG + chr4.3:13373748-13373767 MS.gene065968:CDS 40.0%
GCATTGTTCGAAAGATCAGT+TGG + chr4.3:13373120-13373139 MS.gene065968:CDS 40.0%
GGCAAAGACTTATGAAATGG+TGG + chr4.3:13372566-13372585 MS.gene065968:CDS 40.0%
GTCAAAGTAGAGTTATCCTC+TGG - chr4.3:13373346-13373365 None:intergenic 40.0%
TAAGTCTTTGCCAGAAAAGG+AGG - chr4.3:13372558-13372577 None:intergenic 40.0%
TGCAGAGAATGACTATGAAC+GGG - chr4.3:13372956-13372975 None:intergenic 40.0%
! AGCTAGTGTTGAAGATGCTA+TGG + chr4.3:13373291-13373310 MS.gene065968:CDS 40.0%
! GGGATTTAGGAAAGTTGATC+CGG + chr4.3:13372856-13372875 MS.gene065968:intron 40.0%
! GTGATCATGGTAGATTTTGG+TGG + chr4.3:13373761-13373780 MS.gene065968:CDS 40.0%
! TTATCCTCTGGATTTTCCCT+GGG - chr4.3:13373334-13373353 None:intergenic 40.0%
!! CTCAAGTTGATCCAATCTCT+CGG - chr4.3:13373377-13373396 None:intergenic 40.0%
!! GTAGATTTTGGTGGAACATG+CGG + chr4.3:13373770-13373789 MS.gene065968:CDS 40.0%
AAGAGTCTCCCAAAATGCCA+CGG - chr4.3:13373404-13373423 None:intergenic 45.0%
AAGCCATGGAGAGAAAACGA+AGG + chr4.3:13373236-13373255 MS.gene065968:CDS 45.0%
AGCAAAACCCTTGCGAATTC+CGG - chr4.3:13372660-13372679 None:intergenic 45.0%
CGAAGAGATACCAGCATGAT+TGG + chr4.3:13373077-13373096 MS.gene065968:CDS 45.0%
CGAGAGATTGGATCAACTTG+AGG + chr4.3:13373375-13373394 MS.gene065968:CDS 45.0%
GACGTTAAACACCGAGAGAT+TGG + chr4.3:13373363-13373382 MS.gene065968:CDS 45.0%
GAGACTCTTGCACATGAAGT+TGG + chr4.3:13373415-13373434 MS.gene065968:CDS 45.0%
GATACCAGCATGATTGGGAA+AGG + chr4.3:13373083-13373102 MS.gene065968:CDS 45.0%
GATGCACTCCACGAAACAAT+AGG - chr4.3:13372609-13372628 None:intergenic 45.0%
TGCTGTAAATCTCTTGCCAC+TGG + chr4.3:13373210-13373229 MS.gene065968:CDS 45.0%
TTAAAGCAACCACTTCTCGC+CGG - chr4.3:13373265-13373284 None:intergenic 45.0%
TTTCGTTGTGTGGAATCCTC+CGG + chr4.3:13372638-13372657 MS.gene065968:CDS 45.0%
! AAGTTCTCTGCCTCCTTTTC+TGG + chr4.3:13372545-13372564 MS.gene065968:CDS 45.0%
! CAACCTTCGTTTTCTCTCCA+TGG - chr4.3:13373242-13373261 None:intergenic 45.0%
! GTTATCCTCTGGATTTTCCC+TGG - chr4.3:13373335-13373354 None:intergenic 45.0%
!! AATGGTGGATGATCGATCCT+CGG + chr4.3:13372581-13372600 MS.gene065968:CDS 45.0%
AAAACCCTTGCGAATTCCGG+AGG - chr4.3:13372657-13372676 None:intergenic 50.0%
AATCACCACTGCAGCAACTG+GGG + chr4.3:13373624-13373643 MS.gene065968:CDS 50.0%
AGAATCACCACTGCAGCAAC+TGG + chr4.3:13373622-13373641 MS.gene065968:CDS 50.0%
ATTAACCCCAGTTGCTGCAG+TGG - chr4.3:13373632-13373651 None:intergenic 50.0%
CAGTTCTTGACAGAGGATCC+TGG + chr4.3:13373667-13373686 MS.gene065968:CDS 50.0%
CATGCCTTTCCCAATCATGC+TGG - chr4.3:13373090-13373109 None:intergenic 50.0%
CCACGAAACAATAGGATCCG+AGG - chr4.3:13372601-13372620 None:intergenic 50.0%
CCTCGGATCCTATTGTTTCG+TGG + chr4.3:13372598-13372617 MS.gene065968:CDS 50.0%
CCTTTCAGACTCCGTTAGAG+GGG - chr4.3:13372996-13373015 None:intergenic 50.0%
CTAACATGCCCCATCTGTTC+TGG - chr4.3:13373811-13373830 None:intergenic 50.0%
CTGGGAACAGTTCTTGACAG+AGG + chr4.3:13373660-13373679 MS.gene065968:CDS 50.0%
GAATCACCACTGCAGCAACT+GGG + chr4.3:13373623-13373642 MS.gene065968:CDS 50.0%
GAATCCTCCGGAATTCGCAA+GGG + chr4.3:13372650-13372669 MS.gene065968:CDS 50.0%
GCCTTTCAGACTCCGTTAGA+GGG - chr4.3:13372997-13373016 None:intergenic 50.0%
GGAGAGAAAACGAAGGTTGC+CGG + chr4.3:13373243-13373262 MS.gene065968:CDS 50.0%
GGATAGAGAACAACTCCTCG+TGG + chr4.3:13373051-13373070 MS.gene065968:CDS 50.0%
TCAAGCACAAGCCCCTCTAA+CGG + chr4.3:13372982-13373001 MS.gene065968:CDS 50.0%
TGCCTTTCAGACTCCGTTAG+AGG - chr4.3:13372998-13373017 None:intergenic 50.0%
TTGGCAAATTCCCACTGCTC+CGG - chr4.3:13372878-13372897 None:intergenic 50.0%
TTTGCCCAGGGAAAATCCAG+AGG + chr4.3:13373327-13373346 MS.gene065968:CDS 50.0%
! AGACTTCTGTAGCTTTGCCC+AGG + chr4.3:13373314-13373333 MS.gene065968:CDS 50.0%
! GAAAGTTGATCCGGAGCAGT+GGG + chr4.3:13372865-13372884 MS.gene065968:CDS 50.0%
! GACTTCTGTAGCTTTGCCCA+GGG + chr4.3:13373315-13373334 MS.gene065968:CDS 50.0%
!! TGAGGCATCCGTGGCATTTT+GGG + chr4.3:13373393-13373412 MS.gene065968:CDS 50.0%
!! TTGAGGCATCCGTGGCATTT+TGG + chr4.3:13373392-13373411 MS.gene065968:CDS 50.0%
CCCCTCTAACGGAGTCTGAA+AGG + chr4.3:13372993-13373012 MS.gene065968:CDS 55.0%
GACAGAGGATCCTGGTGCAT+CGG + chr4.3:13373675-13373694 MS.gene065968:CDS 55.0%
GGAATCCTCCGGAATTCGCA+AGG + chr4.3:13372649-13372668 MS.gene065968:CDS 55.0%
GGTATCTCTTCGTCTCCACG+AGG - chr4.3:13373069-13373088 None:intergenic 55.0%
! GGAAAGTTGATCCGGAGCAG+TGG + chr4.3:13372864-13372883 MS.gene065968:CDS 55.0%
! GGATCAACTTGAGGCATCCG+TGG + chr4.3:13373384-13373403 MS.gene065968:CDS 55.0%
ACCAGCTGCTGCTGCTCTTG+AGG + chr4.3:13373573-13373592 MS.gene065968:CDS 60.0%
CTCTCCATGGCTTCTGCCAG+TGG - chr4.3:13373229-13373248 None:intergenic 60.0%
CTTGCCACTGGCAGAAGCCA+TGG + chr4.3:13373222-13373241 MS.gene065968:CDS 60.0%
GCTGCAGTGGTGATTCTCGC+AGG - chr4.3:13373619-13373638 None:intergenic 60.0%
TCCTGTGCTTCCGATGCACC+AGG - chr4.3:13373688-13373707 None:intergenic 60.0%
! TCCTGGTGCATCGGAAGCAC+AGG + chr4.3:13373684-13373703 MS.gene065968:CDS 60.0%
CGAAGGTTGCCGGCGAGAAG+TGG + chr4.3:13373253-13373272 MS.gene065968:CDS 65.0%
GCCTCAAGAGCAGCAGCAGC+TGG - chr4.3:13373577-13373596 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 13372525 13373849 13372525 ID=MS.gene065968
chr4.3 mRNA 13372525 13373849 13372525 ID=MS.gene065968.t1;Parent=MS.gene065968
chr4.3 exon 13372525 13372737 13372525 ID=MS.gene065968.t1.exon1;Parent=MS.gene065968.t1
chr4.3 CDS 13372525 13372737 13372525 ID=cds.MS.gene065968.t1;Parent=MS.gene065968.t1
chr4.3 exon 13372857 13373849 13372857 ID=MS.gene065968.t1.exon2;Parent=MS.gene065968.t1
chr4.3 CDS 13372857 13373849 13372857 ID=cds.MS.gene065968.t1;Parent=MS.gene065968.t1
Gene Sequence

>MS.gene065968

ATGGATGAAGCTGGAAGTTCAAGTTCTCTGCCTCCTTTTCTGGCAAAGACTTATGAAATGGTGGATGATCGATCCTCGGATCCTATTGTTTCGTGGAGTGCATCGAACAAAAGTTTCGTTGTGTGGAATCCTCCGGAATTCGCAAGGGTTTTGCTTCCGAGATTCTTTAAGCACAATAACTTCTCCAGTTTTATCAGGCAGCTGAATACATATGTAAGCAAATTTGATGTCGGTGTTGTATTTGTTGATGCGAATTCTTGTTTTATGTTTATTTTATTTACTGATTCTGGTTTTTGGTTGCTTGAACTGATTCATAGTATATGTATGTGCAGGGATTTAGGAAAGTTGATCCGGAGCAGTGGGAATTTGCCAATGATGATTTTATAAGGGGGCAACCGCATCTGATGAAGAATATTCATAGACGGAAGCCCGTTCATAGTCATTCTCTGCATAATCTTCAAGCACAAGCCCCTCTAACGGAGTCTGAAAGGCAGAGTATGATTGATGAAATAGAGAAGCTGAAACAGGATAGAGAACAACTCCTCGTGGAGACGAAGAGATACCAGCATGATTGGGAAAGGCATGAGATTCAAATGCATTGTTCGAAAGATCAGTTGGAAAAATTGGAACATAAACAACAAAAGATGTTGTCTTCTGTTTCTGAAGCATTGCAGAAACCTATGATTGCTGTAAATCTCTTGCCACTGGCAGAAGCCATGGAGAGAAAACGAAGGTTGCCGGCGAGAAGTGGTTGCTTTAATAATGAAGCTAGTGTTGAAGATGCTATGGAGACTTCTGTAGCTTTGCCCAGGGAAAATCCAGAGGATAACTCTACTTTGACGTTAAACACCGAGAGATTGGATCAACTTGAGGCATCCGTGGCATTTTGGGAGACTCTTGCACATGAAGTTGGCGACAATTTCGTTCACACTCATTCAAACATGGATTTGGATGAATCTACATGTTGTGCTGATAGTTTGTCGATATCTTGTCAGCAACTAGACGGTGAAGTTCGACCTAAGTCACCTGAAATTGACATGAACGTTGAACCAGCTGCTGCTGCTCTTGAGGCTGTTGCAGTAAAAGAACAACCTGCGAGAATCACCACTGCAGCAACTGGGGTTAATGATGTATTCTGGGAACAGTTCTTGACAGAGGATCCTGGTGCATCGGAAGCACAGGAAGTGCAATCAGAAAGAAAAGATAATAGTAGCAGAAAAAATGAAGGAAAACCTAGTGATCATGGTAGATTTTGGTGGAACATGCGGAAATCAAACAATCATCCAGAACAGATGGGGCATGTTAGTCAAGTAGAGAAAATTTAG
Protein sequence

>MS.gene065968.t1

MDEAGSSSSLPPFLAKTYEMVDDRSSDPIVSWSASNKSFVVWNPPEFARVLLPRFFKHNNFSSFIRQLNTYGFRKVDPEQWEFANDDFIRGQPHLMKNIHRRKPVHSHSLHNLQAQAPLTESERQSMIDEIEKLKQDREQLLVETKRYQHDWERHEIQMHCSKDQLEKLEHKQQKMLSSVSEALQKPMIAVNLLPLAEAMERKRRLPARSGCFNNEASVEDAMETSVALPRENPEDNSTLTLNTERLDQLEASVAFWETLAHEVGDNFVHTHSNMDLDESTCCADSLSISCQQLDGEVRPKSPEIDMNVEPAAAALEAVAVKEQPARITTAATGVNDVFWEQFLTEDPGASEAQEVQSERKDNSSRKNEGKPSDHGRFWWNMRKSNNHPEQMGHVSQVEKI