Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066072.t1 | XP_013470213.1 | 99 | 96 | 1 | 0 | 1 | 96 | 230 | 325 | 8.20E-49 | 202.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066072.t1 | Q9LFR7 | 57.3 | 89 | 36 | 1 | 7 | 95 | 237 | 323 | 8.1e-21 | 100.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066072.t1 | G7ZZC3 | 99.0 | 96 | 1 | 0 | 1 | 96 | 230 | 325 | 5.9e-49 | 202.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene066072 | 0.819835 | 9.42E-53 | -1.69E-46 |
| MS.gene049711 | MS.gene066072 | 0.834425 | 3.02E-56 | -1.69E-46 |
| MS.gene051201 | MS.gene066072 | 0.869447 | 3.07E-66 | -1.69E-46 |
| MS.gene052463 | MS.gene066072 | 0.826349 | 2.85E-54 | -1.69E-46 |
| MS.gene05422 | MS.gene066072 | 0.808619 | 2.83E-50 | -1.69E-46 |
| MS.gene056082 | MS.gene066072 | 0.823965 | 1.04E-53 | -1.69E-46 |
| MS.gene057102 | MS.gene066072 | 0.808236 | 3.42E-50 | -1.69E-46 |
| MS.gene059439 | MS.gene066072 | 0.810392 | 1.18E-50 | -1.69E-46 |
| MS.gene061188 | MS.gene066072 | 0.81512 | 1.09E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066072.t1 | MTR_1g111280 | 98.958 | 96 | 1 | 0 | 1 | 96 | 230 | 325 | 1.13e-65 | 201 |
| MS.gene066072.t1 | MTR_7g406880 | 53.061 | 98 | 42 | 3 | 3 | 96 | 235 | 332 | 4.69e-30 | 109 |
| MS.gene066072.t1 | MTR_4g050140 | 42.353 | 85 | 43 | 2 | 9 | 89 | 212 | 294 | 1.30e-14 | 67.4 |
| MS.gene066072.t1 | MTR_7g107040 | 38.043 | 92 | 51 | 2 | 2 | 89 | 206 | 295 | 2.89e-12 | 60.8 |
| MS.gene066072.t1 | MTR_1g089310 | 38.202 | 89 | 53 | 2 | 9 | 95 | 238 | 326 | 2.82e-11 | 58.2 |
| MS.gene066072.t1 | MTR_1g089310 | 38.202 | 89 | 53 | 2 | 9 | 95 | 254 | 342 | 3.20e-11 | 58.2 |
| MS.gene066072.t1 | MTR_7g093950 | 36.264 | 91 | 54 | 1 | 9 | 95 | 242 | 332 | 6.67e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066072.t1 | AT5G16080 | 57.303 | 89 | 36 | 1 | 7 | 95 | 237 | 323 | 4.65e-28 | 103 |
| MS.gene066072.t1 | AT1G68620 | 48.889 | 90 | 42 | 2 | 7 | 96 | 227 | 312 | 2.14e-21 | 86.3 |
| MS.gene066072.t1 | AT3G63010 | 40.000 | 80 | 46 | 2 | 15 | 92 | 245 | 324 | 7.54e-12 | 60.1 |
Find 23 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCAGCTTCAGATACTTAT+TGG | 0.192800 | 1.4:-84937699 | MS.gene066072:CDS |
| GTGTAATCCATTGGTGAAAA+TGG | 0.278139 | 1.4:-84937635 | MS.gene066072:CDS |
| CCTGAGATTTACTTAGAATC+TGG | 0.322976 | 1.4:+84937464 | None:intergenic |
| GTTGAATGTGAAGTGTTTAA+AGG | 0.324790 | 1.4:-84937502 | MS.gene066072:CDS |
| AAATGCATGGAACAATTATC+TGG | 0.405311 | 1.4:-84937736 | None:intergenic |
| TGTGATGCTTTAGGAAGAAC+AGG | 0.432736 | 1.4:-84937532 | MS.gene066072:CDS |
| TGGCGATTGGCATTACCATA+TGG | 0.434796 | 1.4:-84937679 | MS.gene066072:CDS |
| TTCAGATACTTATTGGCGAT+TGG | 0.468433 | 1.4:-84937692 | MS.gene066072:CDS |
| TGTGAAGTGTTTAAAGGTGT+TGG | 0.470256 | 1.4:-84937496 | MS.gene066072:CDS |
| CCAGATTCTAAGTAAATCTC+AGG | 0.474814 | 1.4:-84937464 | None:intergenic |
| CTTTAGGAAGAACAGGTACA+AGG | 0.492026 | 1.4:-84937525 | MS.gene066072:CDS |
| TGATCACGATCTTCACCATA+TGG | 0.493029 | 1.4:+84937664 | None:intergenic |
| ATCTGAGATGGATATATTGA+AGG | 0.500971 | 1.4:-84937572 | MS.gene066072:CDS |
| TATATTGAAGGATAGGAACT+TGG | 0.516422 | 1.4:-84937560 | MS.gene066072:CDS |
| AGATGGATATATTGAAGGAT+AGG | 0.521304 | 1.4:-84937567 | MS.gene066072:CDS |
| TCAGATATACACACCAACAT+AGG | 0.575792 | 1.4:+84937589 | None:intergenic |
| GCTACTGATGATGCCTATGT+TGG | 0.579488 | 1.4:-84937602 | MS.gene066072:CDS |
| TTCACCAATGGATTACACCA+TGG | 0.598695 | 1.4:+84937640 | None:intergenic |
| GTTGGTGTGTATATCTGAGA+TGG | 0.604384 | 1.4:-84937584 | MS.gene066072:CDS |
| TCATCCATGGTGTAATCCAT+TGG | 0.611826 | 1.4:-84937644 | MS.gene066072:CDS |
| TAATCCATTGGTGAAAATGG+AGG | 0.623717 | 1.4:-84937632 | MS.gene066072:CDS |
| GTGAAGATCGTGATCATCCA+TGG | 0.631742 | 1.4:-84937657 | MS.gene066072:CDS |
| TTTAGGAAGAACAGGTACAA+GGG | 0.650756 | 1.4:-84937524 | MS.gene066072:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATGGATATATTGAAGGAT+AGG | - | chr1.4:84937628-84937647 | MS.gene066072:CDS | 30.0% | |
| TATATTGAAGGATAGGAACT+TGG | - | chr1.4:84937635-84937654 | MS.gene066072:CDS | 30.0% | |
| ! | ATCTGAGATGGATATATTGA+AGG | - | chr1.4:84937623-84937642 | MS.gene066072:CDS | 30.0% |
| ! | GTTGAATGTGAAGTGTTTAA+AGG | - | chr1.4:84937693-84937712 | MS.gene066072:CDS | 30.0% |
| GTGTAATCCATTGGTGAAAA+TGG | - | chr1.4:84937560-84937579 | MS.gene066072:CDS | 35.0% | |
| TAATCCATTGGTGAAAATGG+AGG | - | chr1.4:84937563-84937582 | MS.gene066072:CDS | 35.0% | |
| TAGCAGCTTCAGATACTTAT+TGG | - | chr1.4:84937496-84937515 | MS.gene066072:CDS | 35.0% | |
| TCAGATATACACACCAACAT+AGG | + | chr1.4:84937609-84937628 | None:intergenic | 35.0% | |
| TTCAGATACTTATTGGCGAT+TGG | - | chr1.4:84937503-84937522 | MS.gene066072:CDS | 35.0% | |
| TTTAGGAAGAACAGGTACAA+GGG | - | chr1.4:84937671-84937690 | MS.gene066072:CDS | 35.0% | |
| ! | AATTCCTCCATTTTCACCAA+TGG | + | chr1.4:84937570-84937589 | None:intergenic | 35.0% |
| ! | TGTGAAGTGTTTAAAGGTGT+TGG | - | chr1.4:84937699-84937718 | MS.gene066072:CDS | 35.0% |
| !! | TTGGAGTTTTGTGATGCTTT+AGG | - | chr1.4:84937654-84937673 | MS.gene066072:CDS | 35.0% |
| GTTGGTGTGTATATCTGAGA+TGG | - | chr1.4:84937611-84937630 | MS.gene066072:CDS | 40.0% | |
| TCATCCATGGTGTAATCCAT+TGG | - | chr1.4:84937551-84937570 | MS.gene066072:CDS | 40.0% | |
| TGATCACGATCTTCACCATA+TGG | + | chr1.4:84937534-84937553 | None:intergenic | 40.0% | |
| ! | CTTTAGGAAGAACAGGTACA+AGG | - | chr1.4:84937670-84937689 | MS.gene066072:CDS | 40.0% |
| !! | TGTGATGCTTTAGGAAGAAC+AGG | - | chr1.4:84937663-84937682 | MS.gene066072:CDS | 40.0% |
| !! | TTCACCAATGGATTACACCA+TGG | + | chr1.4:84937558-84937577 | None:intergenic | 40.0% |
| GTGAAGATCGTGATCATCCA+TGG | - | chr1.4:84937538-84937557 | MS.gene066072:CDS | 45.0% | |
| TGGCGATTGGCATTACCATA+TGG | - | chr1.4:84937516-84937535 | MS.gene066072:CDS | 45.0% | |
| ! | GCTACTGATGATGCCTATGT+TGG | - | chr1.4:84937593-84937612 | MS.gene066072:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 84937465 | 84937752 | 84937465 | ID=MS.gene066072 |
| chr1.4 | mRNA | 84937465 | 84937752 | 84937465 | ID=MS.gene066072.t1;Parent=MS.gene066072 |
| chr1.4 | exon | 84937465 | 84937752 | 84937465 | ID=MS.gene066072.t1.exon1;Parent=MS.gene066072.t1 |
| chr1.4 | CDS | 84937465 | 84937752 | 84937465 | ID=cds.MS.gene066072.t1;Parent=MS.gene066072.t1 |
| Gene Sequence |
| Protein sequence |
